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1.
Food Chem ; 404(Pt A): 134587, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36257264

RESUMO

With the concern of rapid retrogradation of chestnut starch, the effect of heat-moisture treatment (HMT) with flavonoids on the retrogradation of chestnut starch was studied. The retrogradation of starch could be inhibited by HMT combined with flavonoid addition up to 65.88%. Flavonoids with different structures showed significant differences in retarding retrogradation, which followed the order of epicatechin>epicatechin gallate>oligmeric proanthocyanidins. Structural analysis, infrared spectra and theoretical calculations showed that hydrogen bond interactions between starch and flavonoids retarded starch retrogradation by inhibiting the aggregation of starch molecules to form crystalline and aggregation structures. The retarding effect of flavonoids was related to their steric hindrance, flexibility and phenolic hydroxyl number. Epicatechin with minor steric hindrance and more flexibility favored its hydrogen bond interactions with starch, leading to the best retardation effect of starch retrogradation. This study would provide a theoretical and experimental basis for creating high-quality chestnut products.


Assuntos
Catequina , Amido , Amido/química , Temperatura Alta , Flavonoides , Ligação de Hidrogênio
2.
DEN open ; 3(1): e159, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35959099

RESUMO

Objectives: Between May and July 2021, the coronavirus disease 2019 (COVID-19) pandemic led to a sharp surge in community transmission in Taiwan. We present a three-stage restructuring process of pre-endoscopy triage at the beginning of the pandemic, which can support urgent endoscopic procedures while protecting endoscopy staff. Methods: The pre-endoscopy triage framework was set up with three checkpoints at the hospital entrance, outpatient department, and endoscopy unit, with a specific target patient population and screening methods. Relevant data included the number of endoscopic procedures performed, outpatient department visits, and performing screening methods such as temperature measurement, travel, occupation, contact, and clustering history checking, polymerase chain reaction assay, and rapid antigen test. Results: Forehead temperature measurement and verification of travel, occupation, contact, and clustering history provided rapid, easy, and early mass screening of symptomatic patients at the hospital entrance. During the pandemic, outpatient department visits and endoscopic procedures decreased by 37% and 64%, respectively. The pre-endoscopy screening methods used displayed regional variations in COVID-19 prevalence. Among 16 endoscopy units with a community prevalence of ≥ 31.04 cases per 100,000 residents, 12 (75%) used polymerase chain reaction assay and four (25%) used rapid antigen test to identify asymptomatic patients before endoscopy. Of 6540 pre-endoscopy screening patients, 15 (0.23%) tested positive by laboratory testing. No endoscopy-related nosocomial COVID-19 infections were reported during the pandemic. Conclusions: We present a three-stage pre-endoscopy triage based on the local laboratory capacity, medical resources, and community prevalence. These measures could be useful during the COVID-19 pandemic.

3.
Brain ; 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36343661

RESUMO

Mitochondria are a culprit in the onset of Parkinson's disease, but their role during disease progression is unclear. Here we used Cox proportional hazards models to exam the effect of variation in the mitochondrial genome on longitudinal cognitive and motor progression over time in 4064 patients with Parkinson's disease. Mitochondrial macro-haplogroup was associated with reduced risk of cognitive disease progression in the discovery and replication population. In the combined analysis, patients with the super macro-haplogroup J, T, U# had a 41% lower risk of cognitive progression with P = 2.42 × 10-6 compared to those with macro-haplogroup H. Exploratory analysis indicated that the common mitochondrial DNA variant, m.2706A>G, was associated with slower cognitive decline with a hazard ratio of 0.68 (95% confidence interval 0.56-0.81) and P = 2.46 × 10-5. Mitochondrial haplogroups were not appreciably linked to motor progression. This initial genetic survival study of the mitochondrial genome suggests that mitochondrial haplogroups may be associated with the pace of cognitive progression in Parkinson's disease over time.

4.
J Chromatogr Sci ; 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36349369

RESUMO

Meropenem is a wide inter-individual variability in the pharmacokinetic, and standard dosing may not be adequate in critically ill patients. Therapeutic drug monitoring is a useful tool to optimize dosing. Meropenem is the amphoteric compound with an isoelectric point of 5.15. The secondary amino group of meropenem is positively charged when pH ≤ 5.4, thus we attempted to separate by strong cation exchange (SCX) column using acetonitrile/25-mM potassium dihydrogen phosphate (pH 3.0; 60:40) as mobile phase, and good peak shape and effective separation obtained. Generally, meropenem were unstable in plasma. We try to investigate stability of plasma samples using the medium QC sample with or without 3-(N-morpholino) propanesulfonic acid (MOPS) as stabilizer solutions at possible conditions during handling and storage. Meropenem showed higher stability at -80°C, and addition of MOPS might increase the short-term and extracted samples stability. This method is suitable for the quantification of meropenem in human plasma from 0.5 to 100 µg/mL. The accuracy was ranged from 96.53 to 101.11% with relative standard deviation ≤ 4.76%. The method has been used for determined 63 critically ill patients treated with meropenem. During the first measurement, 11 patients showed trough levels below the target ranges despite standard dosing. Through continuous or prolonged infusion, 8/11 patients (72.73%) led to adequate trough levels. The described SCX-high-performance liquid chromatography method for meropenem in human plasma is a powerful tool for therapeutic drug monitoring.

5.
BMJ Open ; 12(11): e064978, 2022 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-36418139

RESUMO

INTRODUCTION: Neurogenic lower urinary tract dysfunction (NLUTD) both threatens the health of affected patients long-term and also has a significantly negative impact on the patients' quality of life. present, many clinical practice guidelines (CPGs) have been developed for NLUTD patients, but these CPGs may confuse healthcare professionals and patients due to their great difference in terms of scope, quality and content. This review aims to identify the CPGs for NLUTD patients published from 2012 to 2022, assess their quality and then analyse them in an integrated manner. METHODS AND ANALYSIS: We will systematically search electronic healthcare databases (English databases including PubMed, EMBASE, OVID, Scopus, Web of Science, Cochrane Library, CINAHL, UpToDate, and Best Practice and Chinese databases including China National Knowledge Infrastructure, Wanfang Database, VIP Periodical Resource Integration Service Platform and SinoMed), online CPG repositories and relevant professional association websites to identify eligible CPGs. The CPGs published in English and Chinese with full texts available within the period from January 2012 to March 2022 will be included in this study. The Appraisal of Guidelines for Research and Evaluation (AGREE) II will be used to assess the quality of included CPGs. According to the predesigned data table, the general characteristics of these CPGs, proposed recommendations and their quality of evidence, strength of recommendation and other information will be extracted. Qualitative thematic analysis will be applied to the extracted recommendations. A summary of the proposed recommendations, their quality of evidence, strength of recommendation and other information will eventually be described in a table. This review is expected to identify knowledge gaps in current CPGs and to identify the areas of the proposed recommendations derived from low-level evidence. ETHICS AND DISSEMINATION : This systematic review does not involve the participation of any subjects, and therefore no ethical approval is required. The findings of this review will be published in a peer-reviewed journal and disseminated via conference presentations. PROSPERO REGISTRATION NUMBER: CRD42022318180.


Assuntos
Qualidade de Vida , Sistema Urinário , Humanos , China , Bases de Dados Factuais , Revisões Sistemáticas como Assunto
6.
Int J Nanomedicine ; 17: 5353-5374, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36419719

RESUMO

Introduction: Approximately 15%~30% of breast cancers have gene amplification or overexpression of the human epidermal growth factor receptor 2 (HER2), resulting in the chemotherapy resistance, a more-aggressive phenotype and poor prognosis. Methods: We propose a strategy of nanocarriers co-loaded with docetaxel (DTX) and pictilisib (PIC) at a synergistic ratio and non-covalently bound with dual anti-HER2 epitopes bispecific antibodies (BsAbs: anti-HER2-IV/methoxy-polyethylene glycol (mPEG) and anti-HER2-II/methoxy-PEG) for synergistic targeting to overcome the therapeutic dilemmas of the resistance for HER2-targetable chemodrugs. DTX/PIC-loaded nanocarriers (D/P_NCs) were prepared with single emulsion methods and characterized using dynamic light scattering analysis, and the drug content was assayed by high-performance liquid chromatographic method. The integrity and function of BsABs were evaluated using sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). The in vitro cell studies and in vivo breast tumor-bearing mice model were used to evaluate the anti-cancer effect and biosafety of formulations. Results: D/P_NCs optimally prepared exhibited a spherical morphology with small particle sizes (~140 nm), high drug loading (~5.5%), and good colloidal stability. The synergistic tumor cytotoxicity of loading DTX and PIC at 2:1 ratio in D/P_NCs was discovered. The BsAbs are successfully decorated on mPEGylated DTX/PIC-loaded nanocarriers via anti-mPEG moiety. In vitro studies revealed that non-covalent decoration with dual BsAbs on D_P-NCs significantly and synergistically increased cellular uptake, while with loading DTX and PIC at a synergistic ratio of 2:1 in D/P_NCs further resulted in synergistic cytotoxicity. In vivo tumor inhibition studies showed the comparable results for synergistic antitumor efficacy while minimizing systemic toxicity of chemodrugs. Conclusion: Non-covalent modification with dual distinct epitopes BsAbs on the nanocarriers loaded with dual chemodrugs at a synergistic ratio was expected to be a promising therapeutic platform to overcome the chemoresistance of various cancers and warrants further development for future therapy in the clinical.


Assuntos
Antineoplásicos , Neoplasias da Mama , Humanos , Camundongos , Animais , Feminino , Docetaxel , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/metabolismo , Taxoides/química , Antineoplásicos/uso terapêutico , Linhagem Celular Tumoral , Epitopos
7.
Langmuir ; 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36413813

RESUMO

Carbon nanotube (CNT) films have extensive applications due to their excellent electrical, mechanical, and thermal properties. A grand challenge is controlling areal density of CNT films to accommodate various applications. Here, a method based on the Marangoni effect is used to fabricate liquid-supported CNT films with tunable areal density, scalable area, and transferability to arbitrary substrates. By adjusting the viscosity and surface tension of the base liquid media, the Marangoni flow area of surfactant-assisted single-walled CNT (SWCNT) dispersion on the surface of base media was controllable and sparse or dense SWCNT films can be easily obtained. The thickness of the films is controlled by changing the concentration of the SWCNT dispersion. These SWCNT-based transparent-conductive films have widely controllable transmittance and conductivity and exhibit great photoelectric properties (T ∼ 82.4%, Rs ∼ 407 Ω/sq).

8.
Brain Sci ; 12(11)2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36358373

RESUMO

BACKGROUND: After deep brain stimulation (DBS), hiccups as a complication may lead to extreme fatigue, sleep deprivation, or affected prognosis. Currently, the causes and risk factors of postoperative hiccups are unclear. In this study, we investigated the risk factors for hiccups after DBS of the subthalamic nucleus (STN) for Parkinson's disease (PD) under general anesthesia. METHODS: We retrospectively included patients who underwent STN DBS in the study, and collected data of demographic characteristics, clinical evaluations, and medications. According to the occurrence of hiccups within seven days after operation, the patients were divided into a hiccups group and non-hiccups group. The potentially involved risk factors for postoperative hiccups were statistically analyzed by logistic regression analysis. RESULTS: A total of 191 patients were included in the study, of which 34 (17.80%) had postoperative transient persistent hiccups. Binary univariate logistic regression analysis showed that male, higher body mass index (BMI), smoker, Hoehn and Yahr stage (off), preoperative use of amantadine, hypnotic, Hamilton anxiety scale and Hamilton depression scale scores, and postoperative limited noninfectious peri-electrode edema in deep white matter were suspected risk factors for postoperative hiccups (p < 0.1). In binary multivariate logistic regression analysis, male (compared to female, OR 14.00; 95% CI, 1.74-112.43), postoperative limited noninfectious peri-electrode edema in deep white matter (OR, 7.63; 95% CI, 1.37-42.37), preoperative use of amantadine (OR, 3.64; 95% CI, 1.08-12.28), and higher BMI (OR, 3.50; 95% CI, 1.46-8.36) were independent risk factors for postoperative hiccups. CONCLUSIONS: This study is the first report about the risk factors of hiccups after STN DBS under general anesthesia for PD patients. The study suggests that male, higher BMI, preoperative use of amantadine, and postoperative limited noninfectious peri-electrode edema in deep white matter are independent risk factors for postoperative hiccups of STN-DBS for PD patients. Most hiccups after STN-DBS for PD patients were transient and self-limiting.

9.
Entropy (Basel) ; 24(11)2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36359713

RESUMO

This paper presents a method to minimize the spread of negative influence on social networks by contact blocking. First, based on the infection-spreading process of COVID-19, the traditional susceptible, infectious, and recovered (SIR) propagation model is extended to the susceptible, non-symptomatic, infectious, and recovered (SNIR) model. Based on this model, we present a method to estimate the number of individuals infected by a virus at any given time. By calculating the reduction in the number of infected individuals after blocking contacts, the method selects the set of contacts to be blocked that can maximally reduce the affected range. The selection of contacts to be blocked is repeated until the number of isolated contacts that need to be blocked is reached or all infection sources are blocked. The experimental results on three real datasets and three synthetic datasets show that the algorithm obtains contact blockings that can achieve a larger reduction in the range of infection than other similar algorithms. This shows that the presented SNIR propagation model can more precisely reflect the diffusion and infection process of viruses in social networks, and can efficiently block virus infections.

10.
Ageing Res Rev ; 82: 101774, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36332756

RESUMO

Inositol-requiring enzyme 1 α (IRE1α) is a type I transmembrane protein that resides in the endoplasmic reticulum (ER). IRE1α, which is the primary sensor of ER stress, has been proven to maintain intracellular protein homeostasis by activating X-box binding protein 1 (XBP1). Further studies have revealed novel physiological functions of the IRE1α, such as its roles in mRNA and protein degradation, inflammation, immunity, cell proliferation and cell death. Therefore, the function of IRE1α is not limited to its role in ER stress; IRE1α is also important for regulating other processes related to cellular physiology. Furthermore, IRE1α plays a key role in neurodegenerative diseases that are caused by the phosphorylation of Tau protein, the accumulation of α-synuclein (α-syn) and the toxic effects of mutant Huntingtin (mHtt). Therefore, targeting IRE1α is a valuable approach for treating neurodegenerative diseases and regulating cell functions. This review discusses the role of IRE1α in different cellular processes, and emphasizes the importance of IRE1α in neurodegenerative diseases.


Assuntos
Endorribonucleases , Doenças Neurodegenerativas , Humanos , Proteínas Serina-Treonina Quinases/genética , Estresse do Retículo Endoplasmático , Fosforilação
11.
J Org Chem ; 87(22): 15644-15652, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36322841

RESUMO

A palladium-catalyzed asymmetric annulative allylic alkylation reaction of 2-[(1H-indol-2-yl)methyl]malonates with (E)-but-2-ene-1,4-diyl dicarbonates is described, leading to the regio- and enantioselective synthesis of dihydropyrido[1,2-a]indoles with a chiral cyclic allyl stereocenter adjacent to the ring-junction nitrogen atom in moderate to good yields. The salient features of this protocol include mild conditions, a broad substrate scope, and good compatibility with substituents as well as high regio- and stereoselectivities, providing a catalytic asymmetric entry for fabricating chiral pyridoindole scaffolds.


Assuntos
Compostos Alílicos , Estereoisomerismo , Alquilação , Catálise , Indóis
12.
BJOG ; 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36330672

RESUMO

OBJECTIVE: Patients with recurrent endometrial cancer treated with carboplatin and paclitaxel whose disease progresses have few effective treatment options. Based on promising clinical trial data, the anti-programmed cell death 1 (anti-PD-1) antibody dostarlimab was recently granted accelerated approval for endometrial cancer by the US Food and Drug Administration. We developed a decision model to examine the cost-effectiveness of dostarlimab for patients with progressive/recurrent deficient mismatch repair (dMMR) endometrial cancer whose disease has progressed with first-line chemotherapy. DESIGN: Cost-effectiveness study. POPULATION: Hypothetical cohort of 6000 women with progressive/recurrent dMMR endometrial cancer. METHODS: The initial decision point in the Markov model was treatment with dostarlimab, pembrolizumab or pegylated liposomal doxorubicin (PLD). Model probabilities, and cost and utility values were derived with assumptions drawn from published literature. Effectiveness was estimated as average quality-adjusted life years (QALYs) gained. One-way, two-way and probabilistic sensitivity analyses were performed to vary the assumptions across a range of plausible values. MAIN OUTCOME MEASURES: The primary outcome was the incremental cost-effectiveness ratio (ICER). RESULTS: Pegylated liposomal doxorubicin (PLD) was the least costly strategy, at $55,732, followed by dostarlimab ($151,533) and pembrolizumab ($154,597). Based on a willingness-to-pay threshold of $100,000/QALY, PLD was cost-effective compared with dostarlimab, with an ICER of $331,913 per QALY gained for dostarlimab, whereas pembrolizumab was ruled out by extended dominance (less effective, more costly), compared with dostarlimab. In one-way sensitivity analyses, dostarlimab was cost-effective when its cost was reduced to $4905 (52% reduction). These results were robust in a variety of sensitivity analyses. CONCLUSIONS: Dostarlimab is associated with greater survival compared with other treatments for women with recurrent dMMR endometrial cancer. Although the agent is substantially more costly, dostarlimab became cost-effective when its cost was reduced to $5489 per cycle.

13.
Inorg Chem ; 61(46): 18752-18758, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36345055

RESUMO

Here, a hexanuclear Co6(µ3-OH)6 cluster-based metal-organic framework (MOF), [Co6(µ3-OH)6(BTB)2(bpy)3]n (JXNU-15) (bpy = 4,4'-bipyridine), with the 1,3,5-tri(4-carboxyphenyl)benzene (BTB3-) ligand was synthesized for the challenging propadiene/propylene separation. The combination of a large pore volume and a suitable pore environment boosts the significantly high propadiene (C3H4) uptake (311 cm3 g-1 at 298 K and 100 kPa) for JXNU-15. An amine-functionalized MOF of JXNU-15(NH2) was further obtained with the 1,3,5-tri(4-carboxyphenyl)benzene analogue of 3,3″-diamino-5'-(3-amino-4-carboxyphenyl)-[1,1':3',1″-terphenyl]-4,4″-dicarboxylic ligand. The comparative studies of propadiene/propylene(C3H4/C3H6) separation performance between isostructural JXNU-15 and JXNU-15(NH2) are provided. JXNU-15(NH2) exhibits an impressive C3H4 capacity at low pressures with 69.1 cm3 g-1 at 10 kPa, which is twice that of JXNU-15 under the same conditions. Moreover, the separation selectivity of JXNU-15(NH2) is 1.3-fold higher as compared to JXNU-15. JXNU-15(NH2) with enhanced C3H4/C3H6 separation performance was elegantly illustrated by gas separation experiments and theoretical simulations. This work presents an amine-functionalized strategy for the enhancement of the C3H4/C3H6 separation performance of MOF.

14.
Artigo em Inglês | MEDLINE | ID: mdl-36374872

RESUMO

This paper presents an energy-autonomous wireless soil pH and electrical conductance measurement IC powered by soil microbial and photovoltaic energy. The chip integrates highly efficient dual-input, dual-output power management units, sensor readout circuits, a wireless receiver, and a transmitter. The design scavenges ambient energy with a maximal power point tracking mechanism while achieving a peak efficiency of 81.3% and the efficiency is more than 50% over the 0.05-14 mW load range. The sensor readout IC achieves a sensitivity of -8.8 kHz/pH and 6 kHz·m/S, a noise floor of 0.92 x 10-3 pH value, and 1.4 mS/m conductance. To avoid interference, a 433 MHz transceiver incorporates chirp modulation and on-off keying (OOK) modulation for data uplink and downlink communication. The receiver sensitivity is -80 dBm, and the output transmission power is -4 dBm. The uplink data rate is 100 kb/s using burst chirp modulation and gated Class E PA, while the downlink data rate is 10 kb/s with a self-frequency tracking mixer-first receiver.

16.
Antimicrob Agents Chemother ; : e0112922, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377887

RESUMO

Antibiotic exposure is a crucial risk factor for community-acquired Clostridioides difficile infection (CA-CDI). However, the relative risks associated with specific antibiotics may vary over time, and the absolute risks have not been clearly established. This is a retrospective cohort study. Adults were included if they received an outpatient antibiotic prescription within the IBM MarketScan databases between 2008 and 2020. The primary exposure was an outpatient antibiotic prescription, and the receipt of doxycycline was used as the reference comparison. The primary outcome was CA-CDI, defined as the presence of an International Classification of Diseases (ICD) diagnosis code for CDI within 90 days of receiving an outpatient antibiotic prescription, and subsequent treatment for CDI. There were 36,626,794 unique patients who received outpatient antibiotics, including 11,607 (0.03%) who developed CA-CDI. Relative to doxycycline, the antibiotics conferring the highest risks for CA-CDI were clindamycin (adjusted odds ratio [aOR], 8.81; 95% confidence interval [CI], 7.76 to 10.00), cefdinir (aOR, 5.86; 95% CI, 5.03 to 6.83), cefuroxime (aOR, 4.57; 95% CI, 3.87 to 5.39), and fluoroquinolones (aOR, 4.05; 95% CI, 3.58 to 4.59). Among older patients with CA-CDI risk factors, nitrofurantoin was also associated with CA-CDI (aOR, 3.05; 95% CI, 1.92 to 4.84), with a smaller number needed to harm, compared to the fluoroquinolones. While clindamycin, cefuroxime, and fluoroquinolone use declined from 2008 to 2020, nitrofurantoin use increased by 40%. Clindamycin was associated with the greatest CA-CDI risk, overall. Among older patients with an elevated baseline risk for CA-CDI, multiple antibiotics, including nitrofurantoin, had strong associations with CA-CDI. These results may guide antibiotic selection and future stewardship efforts.

17.
Front Immunol ; 13: 1003859, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36353623

RESUMO

Background: Trastuzumab-containing chemotherapy is the first-line treatment for advanced gastric cancer (GC) with HER2 positive. Although PD-1 inhibitors significantly improved the outcome of GC patient's refractory to previous chemotherapy regimens, few studies explore the role of anti-PD-1 therapy overcomes resistance to trastuzumab plus chemotherapy in advanced Epstein-Barr Virus-associated gastric cancer (EBVaGC) with PD-L1 and HER2 positive. Case Presentation: We report a case of advanced EBVaGC in a 45-year-old man presenting with fatigue, dysphagia, and weight loss for several months. Initial endoscopy revealed a large tumor at the gastroesophageal junction. Computed tomography revealed GC accompanied by multiple lymph nodes and hepatic and pulmonary metastases. The immunohistochemistry indicated that HER-2 and PD-L1 were overexpressed, and tumor cells were positive for EBV-encoded small RNA (EBER) by in situ hybridization. Trastuzumab plus DCS was started as first-line chemotherapy with a PFS of 4 months and shifted to trastuzumab plus FOLFIRI or gemcitabine as second-/third-line therapy. After five-cycle nivolumab monotherapy, the patient received partial response and was treated with total radical gastrectomy plus sequential radiotherapy. He continued the postoperative immunotherapy over 30 cycles with a PFS of 28 months. Due to a new abdominal lymph node metastasis confirmed by PET-CT, he received toripalimab as the next-line treatment and achieved complete remission as the best objective response. Summary: We presented an advanced HER2-positive EBVaGC patient with PD-L1 high expression, refractory to trastuzumab plus chemotherapy, and had a durable clinical benefit sequence with a single dose of the PD-1 inhibitor.


Assuntos
Infecções por Vírus Epstein-Barr , Neoplasias Gástricas , Humanos , Masculino , Pessoa de Meia-Idade , Trastuzumab/uso terapêutico , Neoplasias Gástricas/patologia , Herpesvirus Humano 4 , Antígeno B7-H1/genética , Infecções por Vírus Epstein-Barr/complicações , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada
18.
Chemosphere ; 312(Pt 1): 137149, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36356805

RESUMO

Nowadays, eutrophication problem in surface waterbodies has attracted specific attention. Herein, we reported facile synthesis and application of La/Fe engineered bentonite (LFB) for efficient phosphate elimination. Results indicated that bimetallic modified LFB composite could achieve efficient phosphate removal at pH 2-6, and satisfactory selectivity was implied by stable phosphate capturing within the interference of competing species (Cl-, NO3-, HCO3-, SO42-, F- and HA). Pseudo-second-order model could satisfactorily depict the kinetic behavior at different initial concentrations, indicating chemisorption of phosphate on LFB surface. Isotherm study suggested that phosphate adsorption behavior could be fitted well with Sips isotherm equation, indicating that both homogeneous monolayer adsorption and heterogeneous multilayer coverage of phosphate on LFB surface occurred within the investigated conditions. Adsorption thermodynamics implied the spontaneous and endothermic feature of phosphate loading on LFB composite. Characterization analysis confirmed successful La and Fe loading on bentonite, and electrostatic attraction and ligand exchange were the main adsorption mechanism. The high adsorption capacity, cost-effective feature and strong affinity towards phosphate demonstrated certain potential of as-prepared LFB composite for phosphate separation from eutrophic water.

19.
Membranes (Basel) ; 12(11)2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36363599

RESUMO

In this paper, δ-MnO2 with layered structure was prepared by a facile liquid phase method, and exfoliated MnO2 nanosheet (e-MnO2) was obtained by ultrasonic exfoliation, whose surface was negatively charged. Then, positive charges were grafted on the surface of MnO2 nanosheets with a polycation electrolyte of polydiallyl dimethylammonium chloride (PDDA) in different concentrations. A series of e-MnO2@reduced graphene oxide (rGO) composites were obtained by electrostatic self-assembly combined with hydrothermal chemical reduction. When PDDA was adjusted to 0.75 g/L, the thickness of e-MnO2 was ~1.2 nm, and the nanosheets were uniformly adsorbed on the surface of graphene, which shows layer-by-layer morphology with a specific surface area of ~154 m2/g. On account of the unique heterostructure, the composite exhibits good electrochemical performance as supercapacitor electrodes. The specific capacitance of e-MnO2-0.75@rGO can reach 456 F/g at a current density of 1 A/g in KOH electrolyte, which still remains 201 F/g at 10 A/g. In addition, the capacitance retention is 98.7% after 10000 charge-discharge cycles at 20 A/g. Furthermore, an asymmetric supercapacitor (ASC) device of e-MnO2-0.75@rGO//graphene hydrogel (GH) was assembled, of which the specific capacitance achieves 94 F/g (1 A/g) and the cycle stability is excellent, with a retention rate of 99.3% over 10000 cycles (20 A/g).

20.
Front Plant Sci ; 13: 1046597, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36438155

RESUMO

In common bean (Phaseolus vulgaris L.), postharvest seed coat darkening is an undesirable trait that affects crop value. The increased accumulation of proanthocyanidins (PAs) in the seed coat results in darker seeds in many market classes of colored beans after harvest. The precursors of PAs are synthesized in the cytoplasm, and subsequently get glycosylated and then transported to the vacuoles where polymerization occurs. Thus, vacuolar transporters play an important role in the accumulation of PAs. Here, we report that common bean genome contains 59 multidrug and toxic compound extrusion genes (PvMATEs). Phylogenetic analysis of putative PvMATEs with functionally characterized MATEs from other plant species categorized them into substrate-specific clades. Our data demonstrate that a vacuolar transporter PvMATE8 is expressed at a higher level in the pinto bean cultivar CDC Pintium (regular darkening) compared to 1533-15 (slow darkening). PvMATE8 localizes in the vacuolar membrane and rescues the PA deficient (tt12) mutant phenotype in Arabidopsis thaliana. Analysis of PA monomers in transgenic seeds together with wild-type and mutants suggests a possible feedback regulation of PA biosynthesis and accumulation. Identification of PvMATE8 will help better understand the mechanism of PA accumulation in common bean.

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