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1.
Neural Regen Res ; 18(2): 404-409, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35900437

RESUMO

Our previous study demonstrated the potential therapeutic role of human neural stem cell-derived exosomes (hNSC-Exo) in ischemic stroke. Here, we loaded brain-derived neurotrophic factor (BDNF) into exosomes derived from NSCs to construct engineered exosomes (BDNF-hNSC-Exo) and compared their effects with those of hNSC-Exo on ischemic stroke both in vitro and in vivo. In a model of H2O2-induced oxidative stress in NSCs, BDNF-hNSC-Exo markedly enhanced cell survival. In a rat middle cerebral artery occlusion model, BDNF-hNSC-Exo not only inhibited the activation of microglia, but also promoted the differentiation of endogenous NSCs into neurons. These results suggest that BDNF can improve the function of NSC-derived exosomes in the treatment of ischemic stroke. Our research may support the clinical use of other neurotrophic factors for central nervous system diseases.

2.
Biomed Pharmacother ; 153: 113421, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36076485

RESUMO

In folk medicine, Aloe, a genus of Aloaceae, is constantly developed into laxative drugs or products and skin remedies with tremendous popularity worldwide. However, almost all products of Aloe are in roughly processed form. Therefore, developing related products of the active ingredients derived from Aloe is of great medical value. Aloin is a quality standard compound based on the Chinese Pharmacopoeia (CHP). It has a wide range of pharmacological activities, including anti-tumor, anti-inflammatory, anti-osteoporotic, organ-protective, anti-viral, anti-microbial, anti-parasitic, and laxative potentials. Moreover, it regulates blood lipids and glucose and improves neuropathic pain effects, depicting potential to be transformed into promising medicines and healthcare products. In addition to the functional cosmetics and health products of Aloe, the availability, pharmacological activities, pharmacokinetics, formulation studies, and toxicity of aloin were summarized after investigating the literature from PubMed, Google, and other databases. Moreover, significant attention had been paid to the development of aloin-derived medicines and healthcare products. Thus, the present review clarified the possibility of aloin as medicines and healthcare products to develop and utilize Aloe resources.


Assuntos
Aloe , Emodina , Antraquinonas/farmacologia , Anti-Inflamatórios , Antivirais , Atenção à Saúde , Emodina/análogos & derivados , Emodina/farmacologia , Laxantes
3.
Cancer Nurs ; 2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36089673

RESUMO

BACKGROUND: Neurotoxicity is a major adverse effect of chemotherapy in breast cancer (BC) patients. A number of nonpharmacologic interventions are used to alleviate chemotherapy-related cognitive impairment (CRCI), but no studies have compared their effectiveness. OBJECTIVES: The aim of this study was to identify and compare the effectiveness of different nonpharmacologic interventions for CRCI in BC patients. METHODS: A systematic review and network meta-analysis was conducted following the Cochrane guidelines. All randomized controlled trials were searched in the Cochrane Library, PubMed, MEDLINE (via OVID), Web of Science, EMBASE, and CINAHL databases from inception to September 2021. Studies using nonpharmacologic interventions to manage CRCI symptoms were included. A network meta-analysis and a comparative effects ranking were completed by STATA v14.0. RESULTS: Twelve studies with 8 nonpharmacologic interventions were included. For subjective outcomes on CRCI, there was no significant difference between nonpharmacologic interventions. For objective outcomes, qigong and exercise were more effective than the psychotherapy. Qigong and exercise were also more effective than music therapy. The top 3 interventions were psychotherapy (83.4%), music therapy (60.8%), and electroacupuncture (52.5%) for subjective outcomes and qigong (87.7%), exercise (82.1%), and electroacupuncture (70.3%) for objective outcomes. CONCLUSION: In the subjective evaluation, it was difficult to judge which interventions are best, but psychotherapy had the greatest probability. For objective evaluation, qigong and exercise may be the best nonpharmacologic interventions. IMPLICATIONS FOR PRACTICE: This study provides evidence for the effectiveness of nonpharmacologic interventions for CRCI in BC patients and facilitates support for future clinical trials and work.

4.
Crit Rev Food Sci Nutr ; : 1-31, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36073729

RESUMO

Non-communicable diseases (NCDs) are a global epidemic with diverse pathogenesis. Among them, oxidative stress and inflammation are the most fundamental co-morbid features. Therefore, multi-targets and multi-pathways therapies with significant anti-oxidant and anti-inflammatory activities are potential effective measures for preventing and treating NCDs. The flavonol glycoside compound hyperoside (Hyp) is widely found in a variety of fruits, vegetables, beverages, and medicinal plants and has various health benefits, especially excellent anti-oxidant and anti-inflammatory properties targeting nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB) signaling pathways. In this review, we summarize the pathogenesis associated with oxidative stress and inflammation in NCDs and the biological activity and therapeutic potential of Hyp. Our findings reveal that the anti-oxidant and anti-inflammatory activities regulated by Hyp are associated with numerous biological mechanisms, including positive regulation of mitochondrial function, apoptosis, autophagy, and higher-level biological damage activities. Hyp is thought to be beneficial against organ injuries, cancer, depression, diabetes, and osteoporosis, and is a potent anti-NCDs agent. Additionally, the sources, bioavailability, pharmacy, and safety of Hyp have been established, highlighting the potential to develop Hyp into dietary supplements and nutraceuticals.

5.
Contrast Media Mol Imaging ; 2022: 7642511, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36051936

RESUMO

Objective: To explore the CT radiomic features and clinical imaging features of the primary tumor in patients with nonsmall cell lung cancer (NSCLC) before treatment and their predictive value for the occurrence of bone metastases. Methods: From June 2017 to June 2021, 195 patients with NSCLC who were pathologically diagnosed without any treatment in the Cancer Hospital Affiliated to Hainan Medical College were retrospectively analyzed, and they were divided into a bone metastasis group and a nonbone metastasis group. The relationship between clinical imaging features and bone metastasis in patients was analyzed by the t-test, rank sum test, and χ 2 test. At the same time, ITK software was used to extract the radiomic characteristics of the primary tumor of the patients, and the patients were randomly divided into a training group and a validation group in a ratio of 7 : 3. The training model was validated in the validation group, and the performance of the model for predicting bone metastases in NSCLC patients was verified by the ROC curve, and a multivariate logistic regression prediction model was established based on the omics parameters extracted from the best prediction model combined with clinical image features. Results: Seven features were screened from the primary tumor by LASSO to establish a model for predicting metastasis. The area under the curve was 0.82 and 0.73 in the training and validation sets. The best omics signature and univariate analysis suggested clinical imaging factors (P < 0.05) associated with bone metastases were included in multivariate binary logistic analysis to obtain clinical characteristics of the primary tumor such as gender (OR = 0.141, 95% CI: 0.022-0.919, P = 0.04), increased Cyfra21-1 (OR = 0.12, 95% CI: 0.018-0.782, P = 0.027), Fe content in blood (OR = 0.774, 95% CI: 0.626-0.958, P = 0.018), CT signs such as lesion homogeneity (OR = 0.052, 95% CI: 0.006-0.419, P = 0.006), pleural indentation sign (OR = 0.007, 95% CI: 0.001-0.696, P = 0.034), and omics characteristics glszm_Small Area High Gray Level Emphasis (OR = 0.016, 95% CI: 0.001-0.286, P = 0.005) were independent risk factors for bone metastasis in patients. Conclusion: The prediction model established based on radiomics and clinical imaging features has high predictive performance for the occurrence of bone metastasis in NSCLC patients.


Assuntos
Neoplasias Ósseas , Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Antígenos de Neoplasias , Neoplasias Ósseas/diagnóstico por imagem , Carcinoma Pulmonar de Células não Pequenas/diagnóstico por imagem , Carcinoma Pulmonar de Células não Pequenas/patologia , Humanos , Queratina-19 , Neoplasias Pulmonares/diagnóstico , Valor Preditivo dos Testes , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
6.
Cancer Cell ; 40(9): 1044-1059.e8, 2022 09 12.
Artigo em Inglês | MEDLINE | ID: mdl-36099882

RESUMO

Cisplatin-based chemotherapy remains the primary treatment for unresectable and metastatic muscle-invasive bladder cancers (MIBCs). However, tumors frequently develop chemoresistance. Here, we established a primary and orthotopic MIBC mouse model with gene-edited organoids to recapitulate the full course of chemotherapy in patients. We found that partial squamous differentiation, called semi-squamatization, is associated with acquired chemoresistance in both mice and human MIBCs. Multi-omics analyses showed that cathepsin H (CTSH) is correlated with chemoresistance and semi-squamatization. Cathepsin inhibition by E64 treatment induces full squamous differentiation and pyroptosis, and thus specifically restrains chemoresistant MIBCs. Mechanistically, E64 treatment activates the tumor necrosis factor pathway, which is required for the terminal differentiation and pyroptosis of chemoresistant MIBC cells. Our study revealed that semi-squamatization is a type of lineage plasticity associated with chemoresistance, suggesting that differentiation via targeting of CTSH is a potential therapeutic strategy for the treatment of chemoresistant MIBCs.


Assuntos
Carcinoma de Células Escamosas , Neoplasias da Bexiga Urinária , Animais , Carcinoma de Células Escamosas/tratamento farmacológico , Diferenciação Celular , Cisplatino , Humanos , Camundongos , Neoplasias da Bexiga Urinária/tratamento farmacológico , Neoplasias da Bexiga Urinária/genética , Neoplasias da Bexiga Urinária/patologia
7.
Biomaterials ; 289: 121758, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36049426

RESUMO

Tumor-targeting peptides have profound clinical implications in early detection and delineation of microscopic lesions for surgical resection, and also delivery of therapeutics with reduced systemic toxicity. Here, we demonstrate that a peptide (RS), evolved from a previously reported hepatocellular carcinoma (HCC)-targeting peptide P47, enables improved HCC micrometastasis discrimination and delineation from noncancerous tissues in murine orthotopic mice and patient biopsies, with up to 21-fold contrast. Importantly, RS targets non-small cell lung (NSCLC) and colon cancers in mice and patient biopsies, with higher selectivity for highly proliferative tumor nodules. Moreover, RS localizes to cell nucleoli of HCC, NSCLC, breast, colon and cervical cancer cells and induces nucleolar stress when conjugated with chemotherapeutic Oxaliplatin (OXA) (RS-OXA), demonstrating both cellular and subcellular targeting. RS-delivered OXA elicits significant tumor retardation in orthotopic HCC mice with markedly reduced systemic toxicity compared to OXA alone. Injection of fluorescence-labeled RS enables dynamic visualization of tumor growth in RS-OXA-treated subcutaneous HCC mice. Our study demonstrates that RS targets a spectrum of tumors and localizes to cell nucleolus, thus enabling functional imaging and targeted delivery of OXA in HCC mice, and consequently provides a versatile tool for tumor imaging and targeted therapeutics.

8.
Fish Shellfish Immunol ; 129: 85-95, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36057428

RESUMO

Triclocarban (TCC), an antimicrobial ingredient in personal care products, is associated with immunosuppression and physiological dysfunctions of aquatic organisms. The aim of this study was to investigate whether TCC can induce common carp NETosis (neutrophil death by neutrophil extracellular trap (NET) release) and then to attempt to identify the potential molecular mechanisms. Herein, scanning electron microscopy and flow cytometric assays showed that revealed that TCC triggers DNA-containing web-like structures and increases extracellular DNA content. In the proteomic analysis, we observed that NET-related proteins, extracellular regulated protein kinase (Mapk1, Mapk14, Jak2) and apoptotic protein (caspase3) were significantly increased, and defender against cell death 1 (Dad1) was significantly decreased after TCC treatments. Meanwhile, we confirmed that TCC stress can trigger NETosis in common carp by activating the reactive oxygen species (ROS)/ERK1/2/p38 signaling. We think that the upregulated NDUFS1 expression is closely related to oxidative stress induced by TCC. Importantly, we discovered that SIRT3 expression was significantly decreased in the process of TCC-induced NETs. Importantly, pretreatment with the SIRT3 agonist honokiol (HKL) effectively suppressed TCC-induced NET release. In contrast, the SIRT3 antagonist 3-TYP escalated TCC-induced NET formation. Mechanistically, SIRT3 degradation serves as a potential mediator for regulating oxidative stress crosstalk between ERK1/2/p38 signals in the process of TCC-induced NET formation. These findings unveil new insights into the TCC-evoked health risk of fish and other aquatic organisms and suggest that SIRT3 is a potential pharmacological intervention target to alleviate TCC-induced common carp NETosis.


Assuntos
Carpas , Armadilhas Extracelulares , Proteína Quinase 14 Ativada por Mitógeno , Sirtuína 3 , Animais , Carbanilidas , Carpas/genética , Carpas/metabolismo , Sistema de Sinalização das MAP Quinases , Proteína Quinase 14 Ativada por Mitógeno/metabolismo , Neutrófilos , Proteômica , Espécies Reativas de Oxigênio/metabolismo , Sirtuína 3/metabolismo , Sirtuína 3/farmacologia
9.
Huan Jing Ke Xue ; 43(9): 4438-4447, 2022 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-36096584

RESUMO

The Air Pollution Prevention and Control Action Plan and Three-year Plan on Defending the Blue Sky promulgated by the State Council of the People's Republic of China have played an important role in the overall improvement of air quality in China. However, few studies have evaluated the implementation effects of these two policies in Sichuan Basin and the new characteristics of PM2.5 chemical components after the implementation of these policies. The key periods for evaluating the implementation effects of these two pollution reduction policies are 2017 and 2020, respectively. In order to study the atmospheric PM2.5 and carbonaceous species in Chengdu during these two periods, this study sampled the PM2.5 in Chengdu from October 2016 to July 2017 and December 2020, respectively, and the organic carbon (OC) and elemental carbon (EC) were analyzed. The results showed that the annual ρ(PM2.5) from 2016-2017 in Chengdu was (114.0±76.4) µg·m-3. The maximum value of the ρ(PM2.5) appeared in winter[(193.3±98.5) µg·m-3], and the minimum value appeared in spring[(73.8±32.3) µg·m-3]. By contrast, the ρ(PM2.5) in winter decreased significantly in 2020, with a value of (96.0±39.3) µg·m-3. The annual ρ(OC) and ρ(EC) from 2016-2017 were (21.1±16.4) µg·m-3 and (1.9±1.3) µg·m-3, which accounted for 18.5% and 1.7% of the PM2.5 mass, respectively. The seasonal variation characteristic of ρ(OC) was:winter[(40.6±21.5) µg·m-3]>autumn[(17.0±7.0) µg·m-3]>summer[(14.4±3.9) µg·m-3]>spring[(12.6±6.0) µg·m-3], whereas the ρ(EC) in the four seasons were close, ranging from 1.3 to 2.4 µg·m-3. The annual ρ(SOC) was (9.4±9.1) µg·m-3, which accounted for 44.5% of the OC mass. Compared with that in winter 2016, the ρ(OC) decreased by 52.7% in winter 2020, whereas the ρ(EC) increased by 26.1%. With the aggravation of pollution, the change trends of carbon species and their contributions were different. Compared with that in winter 2016, the variation in the contribution of OC with the aggravation of pollution in winter 2020 was more stable, whereas the proportion of SOC increased more obviously. There were obvious differences in the direction of air masses and the potential source area of pollutants in each season. Although there was no significant change in the direction of air masses in winter 2020 compared with those in winter 2016, the pollutant concentrations corresponding to each cluster decreased significantly, and the potential source area of pollutants expanded significantly to the eastern area.


Assuntos
Poluentes Atmosféricos , Material Particulado , Aerossóis/análise , Poluentes Atmosféricos/análise , Carbono/análise , Monitoramento Ambiental/métodos , Humanos , Tamanho da Partícula , Material Particulado/análise
10.
Peptides ; 157: 170878, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36108979

RESUMO

G protein-coupled receptors (GPCRs) are the largest family of membrane receptors that mediate the effects of cardiac diseases. GPR30, also named G-protein-coupled estrogen receptor, shows beneficial effect on female patients with heart failure. This research aimed to probe the role and mechanism of GPR30 in myocardial hypertrophy. The model of cardiac hypertrophy was induced by infusion of angiotensin (Ang) II in mice, and was induced by Ang II treatment in neonatal rat cardiomyocyte (NRCM). The mouse model of myocardial hypertrophy was induced by angiotensin (Ang) Ⅱ, and the neonatal rat cardiomyocyte (NRCM) was induced by Ang Ⅱ treatment. GPR30 agonist G1 reduced cardiac hypertrophy induced by Ang II in mice, and reduced cardiac atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and ß-myosin heavy chain (ß-MHC) induced by Ang II. Ang Ⅱ treatment of myocardial fibrosis in mice was suppressed after administration of G1. GPR30 deficiency produced the opposite results. Oxidative stress and apoptosis were enhanced in the mice heart induced by Ang II, which were suppressed by G1 administration, but were further exacerbated after GPR30 deficiency. The outcomes demonstrated that GPR30 participated in the regulation of cardiac hypertrophy and fibrosis. Activation of GPR30 ameliorated cardiac hypertrophy and fibrosis by reducing oxidative stress and apoptosis.

11.
Front Pharmacol ; 13: 999179, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36147340

RESUMO

Intestinal macrophages are the main participants of intestinal immune homeostasis and intestinal inflammation. Under different environmental stimuli, intestinal macrophages can be polarized into classical activated pro-inflammatory phenotype (M1) and alternative activated anti-inflammatory phenotype (M2). Its different polarization state is the "guide" to promoting the development and regression of inflammation. Under normal circumstances, intestinal macrophages can protect the intestine from inflammatory damage. However, under the influence of some genetic and environmental factors, the polarization imbalance of intestinal M1/M2 macrophages will lead to the imbalance in the regulation of intestinal inflammation and transform the physiological inflammatory response into pathological intestinal injury. In UC patients, the disorder of intestinal inflammation is closely related to the imbalance of intestinal M1/M2 macrophage polarization. Therefore, restoring the balance of M1/M2 macrophage polarization may be a potentially valuable therapeutic strategy for UC. Evidence has shown that traditional Chinese medicine (TCM) has positive therapeutic effects on UC by restoring the balance of M1/M2 macrophage polarization. This review summarizes the clinical evidence of TCM for UC, the vital role of macrophage polarization in the pathophysiology of UC, and the potential mechanism of TCM regulating macrophage polarization in the treatment of UC. We hope this review may provide some new enlightenment for the clinical treatment, fundamental research, and research and development of new Chinese medicine of UC.

12.
J Evid Based Med ; 2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36151612

RESUMO

Severe mechanical ocular trauma with no light perception (NLP) predicts a poor prognosis of visual acuity and enucleation of the eyeball. Since the innovative treatment concept of exploratory vitreoretinal surgery has developed and treatment technology has advanced, the outcomes of severe ocular trauma treatment in NLP patients have greatly improved. However, there remains a lack of unified standards for the determination, surgical indication, and timing of vitrectomy in NLP eye treatment. To address these problems, we aimed to create evidence-based medical guidelines for the diagnosis, treatment, and prognosis of mechanical ocular trauma with NLP. Sixteen relevant recommendations for mechanical ocular trauma with NLP were obtained, and a consensus was reached. Each recommendation was explained in detail to guide the treatment of mechanical ocular trauma associated with NLP.

13.
Diagnostics (Basel) ; 12(9)2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36140599

RESUMO

BACKGROUND: Pressure injuries (PIs) generally result from prolonged ischemia through localized skin compression, and ischemia persists and exacerbates damage even post-decompression. The mechanisms of ischemia post-decompression are still unclear, and appropriate methods for detection are lacking. METHODS: We used blanchable erythema (BE) and early PI rat models. We assessed the perfusion using Evans Blue (EB) and thrombus formation under a light microscope. Furthermore, we performed a capillary refill time test (CRTT) to detect ischemia after depression coupled with the transparent disk method using a spectrophotometer. RESULTS: Compared with the BE group, the early PI group showed significantly slow and insufficient perfusion, as determined by EB staining (p < 0.001). Histological observations revealed that ischemia during post-decompression of early PI was caused by a greater amount of thrombi. The CRTT results showed that although both groups exhibited varying degrees of insufficient refilling volume, the early PI group had significantly slower refilling than the BE group (p < 0.001), which persisted during the deterioration or disappearance of erythema. CONCLUSIONS: Our results showed that persistent ischemia caused by thrombi is an important cause of early PI deterioration post-decompression. Therefore, the performance of CRTT coupled with the transparent disc method may become a promising method for detecting ischemia post-decompression.

14.
Sensors (Basel) ; 22(18)2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36146290

RESUMO

In this paper, we proposed a novel expectation-maximization-based simultaneous localization and mapping (SLAM) algorithm for millimeter-wave (mmW) communication systems. By fully exploiting the geometric relationship among the access point (AP) positions, the angle difference of arrival (ADOA) from the APs and the mobile terminal (MT) position, and regarding the MT positions as the latent variable of the AP positions, the proposed algorithm first reformulates the SLAM problem as the maximum likelihood joint estimation over both the AP positions and the MT positions in a latent variable model. Then, it employs a feasible stochastic approximation expectation-maximization (EM) method to estimate the AP positions. Specifically, the stochastic Monte Carlo approximation is employed to obtain the intractable expectation of the MT positions' posterior probability in the E-step, and the gradient descent-based optimization is used as a viable substitute for estimating the high-dimensional AP positions in the M-step. Further, it estimates the MT positions and constructs the indoor map based on the estimated AP topology. Due to the efficient processing capability of the stochastic approximation EM method and taking full advantage of the abundant spatial information in the crowd-sourcing ADOA data, the proposed method can achieve a better positioning and mapping performance than the existing geometry-based mmW SLAM method, which usually has to compromise between the computation complexity and the estimation performance. The simulation results confirm the effectiveness of the proposed algorithm.

15.
Int J Ophthalmol ; 15(9): 1423-1430, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36124184

RESUMO

AIM: To identify potential mutations and elucidate the clinical findings of male patients and female carriers of X-linked retinitis pigmentosa (XLRP) in a Chinese family. METHODS: A four generation pedigree was collected that consisted of 20 individuals. Genomic DNA was extracted from peripheral blood, and then the target fragments were amplified by PCR and sequenced directly. In addition, all affected patients and female carriers underwent comprehensively ophthalmic evaluation. RESULTS: A novel mutation c.2865G>A p.W955X in RPGR gene was identified of this family, including four affected individuals and eight carriers. All male patients, aging from 7 to 31y, tended to have more various, even potentially deleterious clinical features of RP. At the same time, individuals with heterozygous mutations (carriers) manifested a wide spectrum of clinical features. Herein, only two male patients and three female carriers manifested pathological myopia (PM). Among the female carriers, half of subjects who harbor poor visual acuity suffered esotropia or exotropia. Additionally, 16.7% and 66.7% of carriers had abnormal electroretinogram (ERG) and fundus, respectively. CONCLUSION: In this study, a novel mutation of the RPGR gene is identified, which broadens the spectrum of RPGR mutations, and elaborates the relationship between genotype and phenotype.

16.
J Med Virol ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36127294

RESUMO

BACKGROUND: Little information is available for antibody levels against SARS-CoV-2 variants of concern induced by Omicron breakthrough infection and a third booster with an inactivated vaccine (InV) or Ad5-nCoV in people with completion of two InV doses. METHODS: Plasma was collected from InV pre-vaccinated Omicron infected patients (OIPs), unvaccinated OIPs between 0-22 days, and healthy donors (HDs) 14 days or 6 months after the second doses of an InV and 14 days after a homogenous booster or heterologous booster of Ad5-nCoV. Anti-Wuhan-, Anti-Delta-, and Anti-Omicron-receptor binding domain (RBD)-IgG titers were detected using enzyme-linked immunosorbent assay. RESULTS: InV pre-vaccinated OIPs had higher anti-Wuhan-, anti-Delta-, and anti-Omicron-RBD-IgG titers compared to unvaccinated OIPs. Anti-Wuhan-RBD-IgG titers sharply increased in InV pre-vaccinated OIPs 0-5 days post-infection (DPI), while the geometric mean titers (GMTs) of anti-Delta- and anti-Omicron-RBD-IgG were 3.3- and 12.0-fold lower. Then, the GMT of anti-Delta- and anti-Omicron-RBD-IgG increased to 35112 and 28186 during 11-22 DPI, about 2.6- and 3.2-fold lower, respectively, than the anti-Wuhan-RBD-IgG titer. The anti-Wuhan-, anti-Delta-, and anti-Omicron-RBD-IgG titers declined over time in HDs after two doses of an InV, with 25.2-, 5.6-, and 4.5-fold declination, respectively, at 6 months relative to the titers at 14 days after the second vaccination. Anti-Wuhan-, anti-Delta-, and anti-Omicron-RBD-IgG titers elicited by a heterologous Ad5-nCoV booster were significantly higher than those elicited by an InV booster, comparable to those in InV pre-vaccinated OIPs. CONCLUSION: InV and Ad5-nCoV booster could improve humoral immunity against Omicron variants. Of these, the Ad5-nCoV booster is a better alternative. This article is protected by copyright. All rights reserved.

17.
World Neurosurg ; 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36122858

RESUMO

OBJECTIVE: To investigate the incidence, distribution characteristics, risk factors, and clinical outcomes of intraoperative endplate injury following transforaminal lumbar interbody fusion (TLIF). METHODS: Patients who underwent single-level TLIF from January 2018 to December 2020 were included. The patients were separated into endplate injury group (EI group) and no endplate injury group (non-EI group) by postoperative computed tomography scanning immediately. All patients' demographic, clinical, and radiographic parameters were analysed. Clinical outcomes were evaluated by visual analogue scale (VAS) for low back pain and Oswestry Disability Index (ODI). RESULTS: 576 patients were enrolled in this study. The rates of endplate injury were 19.6% (113 of 576) of patients and 9.9% (114 of 1152) of endplates. The rate of superior endplate injury was significantly higher than that of inferior endplate injury. The results illustrated that older age, lower disc height index (DHI), and taller cage height were independent risk factors for intraoperative endplate injury. The postoperative drain output, total blood loss, postoperative duration of drainage tube, and postoperative hospital stay in EI group were significantly more than those in non-EI group. There were no statistical differences in ODI and VAS scores at the same time point between two groups. CONCLUSIONS: The rates of endplate injury were 19.6% of patients and 9.9% of endplates. The superior endplates are more susceptible to injury than inferior endplates. Older age, lower DHI, and taller cage height were independent risk factors for intraoperative endplate injury. Clinical outcomes were not affected by intraoperative endplate injury during early postoperative follow-up.

18.
Chemosphere ; 308(Pt 2): 136352, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36088966

RESUMO

The synergistic control of ozone and fine particulate matter is a research hotspot in the current environmental fields. Among the ozone removal, wide humidity fluctuation and low concentration dynamic adsorption are two thorny problems. In this work, MIL-100(Fe) and MIL-100(Mn), synthesized by hydrothermal and solvothermal methods respectively, were selected to investigate the degradation of flowing ozone pollutants. The samples showed different ozone degradation mechanisms, namely photocatalytic degradation and normal temperature degradation. Notably, MIL-100(Fe) exhibited more outstanding photocatalytic activity than MIL-100(Mn), while the normal temperature catalytic efficiency of MIL-100(Mn) was much superior to MIL-100(Fe). For different humidity conditions, MIL-100(Fe) has the optimal photocatalytic performance at 10% humidity, which is 38%, while MIL-100(Mn) has basically no change in normal temperature catalytic degradation efficiency at different humidity levels of 10-90%. Furthermore, the degradation mechanism was proposed by in-situ DRIFTS and ESR, which was significantly correlated with oxygen vacancy and photogenerated electron efficiency. By the aid of Temperature Programmed Desorption (TPD), a large quantity of Lewis acid sites was detected in MIL-100(Mn), which was the critical factor that the selected materials could maintain excellent normal temperature degradation performance under high humidity. This work will expand the practical application of ozone removal and improve the degradation efficiency.

19.
Sci Total Environ ; 854: 158739, 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36108844

RESUMO

Many studies have confirmed groundwater phosphorus (P) enrichment by anthropogenic and geogenic sources. However, the effects of colloidal iron (Fe) and manganese (Mn) on the groundwater P distribution remain poorly-understood. This study investigated the spatial distribution of three forms of Fe, Mn, and P (particulate, colloidal, and truly soluble) in aquifers based on groundwater monitoring data and sediment core samples for the Jianghan Plain. High proportions of colloidal Fe, Mn, and P of up to 52%, 58%, and 76%, respectively were found in the phreatic and confined aquifers. Particulate and truly soluble P dominated the phreatic aquifer and the confined aquifer, respectively. However, the truly soluble Fe and Mn were dominant among the three forms in both the phreatic and confined aquifers. The distributions of Fe, Mn, and P in colloids and sediments were also studied by X-ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS). A comparison of the distributions of Fe, Mn, and P between site SD01 (riparian zones) and site SD02 (farmland) showed that both external inputs and the reduced release of Fe/Mn oxides/minerals from sediments contributed to the distributions of colloidal Fe, Mn, and P. Correlation analysis showed a strong relationship between colloidal Fe/Mn and P in both groundwater and sediment, implying that colloidal Fe/Mn play a role in regulating the distribution of P in the study area. This study provides a new understanding of the effects of colloidal Fe and Mn on the P distribution among the phreatic and confined aquifers.

20.
Lung Cancer ; 173: 43-48, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36116169

RESUMO

INTRODUCTION: Extensive stage small cell lung cancer (ES-SCLC) is associated with poor prognosis. Recently, anlotinib has demonstrated significant clinical activity as a third-line or further on treatment. This study aimed to evaluate the safety and efficacy of a combination of anlotinib and platinum-etoposide as first-line treatment in patients with ES-SCLC. METHODS: The present multi-center, single-arm, prospective study (NCT04684017) was conducted at three Chinese sites, and included patients with asymptomatic metastasis in the central nervous system. Patients were treated with up to six cycles of chemotherapy comprising etoposide with either carboplatin or cisplatin on day 1 of each cycle. Anlotinib was administered orally once daily on days 1-14 per cycle. The primary end points of the study were safety and investigator assessed objective response rate (ORR). RESULTS: A total of 101 patients were screened from August 2018 to September 2021, of which 86 who had received at least one dose of the treatment were included in the formal analysis. The median follow-up duration was 27.9 months. Complete response and partial response were observed in 2 and 73 patients, respectively, with an ORR of 87.2 % and a disease control rate of 97.7 %. Progression-free survival (PFS) and overall survival (OS) events occurred in 78 and 47 patients, respectively. The median PFS and OS were 9.0 (95 % confidence interval [CI]: 7.5-10.5) and 19 (95 % CI: 16.7-21.3) months, respectively. The incidence of grade 3 or higher adverse events (AEs) was 58.1 % and 24 patients (27.9 %) experienced serious treatment-related AEs. No fatalities consequent to AEs were recorded. CONCLUSION: Given its promising efficacy, safety profile and durability, anlotinib combined with chemotherapy deserves further investigation as first-line anticancer therapy in ES-SCLC (NCT: 04684017).

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