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1.
J Hazard Mater ; 441: 129884, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36084465

RESUMO

The removal of trivalent arsenic (As (III)) from water has received extensive attention from researchers. Iron electrocoagulation (Fe-EC) is an efficient technology for arsenic removal. However, electrode passivation hinders the development and application of Fe-EC. In this work, an innovative Fe-EC route was developed to remove As (III) through an electrochemical-siderite packed column (ESC). Ferrous ions were produced from siderite near the anode, and hydroxide was generated near the cathode during the electrochemical decomposition of siderite. As a result, an effect of Fe-EC-like was obtained. The results showed that an excellent removal performance of As (III) (>99%) was obtained by adjusting the parameters (As (III) concentration at 10 mg/L, pH at 7, Na2SO4 at 10 mM and the hydraulic retention time at 30 min) and the oxidation rate of As (III) reached 84.12%. The mechanism analysis indicated that As (III) was oxidized to As (Ⅴ) by the produced active oxide species and electrode, and then was removed by capturing on the iron oxide precipitates. As (III) was likely to be oxidized in two ways, one by the reactive oxygen species (possibly •OH, Fe(IV) and •O2- species), and another directly by the anode. The long-term effectiveness of arsenic removal demonstrated that ESC process based on the electrochemical-siderite packed column was an appropriate candidate for treating As (III) pollution.


Assuntos
Arsênio , Poluentes Químicos da Água , Purificação da Água , Arsênio/química , Carbonatos , Compostos Férricos , Floculação , Ferro/química , Oxirredução , Óxidos , Espécies Reativas de Oxigênio , Água , Poluentes Químicos da Água/química , Purificação da Água/métodos
2.
Life Sci Alliance ; 6(2)2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36414375

RESUMO

Sry on the Y chromosome is the master switch of sex determination in mammals. It has been well established that Sry encodes a transcription factor that is transiently expressed in somatic cells of the male gonad, leading to the formation of testes. In the testis of adult mice, Sry is expressed as a circular RNA (circRNA) transcript. However, the physiological function of Sry circRNA (circSRY) remains unknown since its discovery in 1993. Here we show that circSRY is mainly expressed in the spermatocytes, but not in mature sperm or somatic cells of the testis. Loss of circSRY led to germ cell apoptosis and the reduction of sperm count in the epididymis. The level of γH2AX was decreased, and failure of XY body formation was noted in circSRY KO germ cells. Further study demonstrated that circSRY directly bound to miR-138-5p in spermatocytes, and in vitro assay suggested that circSRY regulates H2AX mRNA through sponging miR-138-5p. Our study demonstrates that, besides determining sex, Sry also plays an important role in spermatogenesis as a circRNA.


Assuntos
MicroRNAs , RNA Circular , Masculino , Camundongos , Animais , Proteína da Região Y Determinante do Sexo/genética , RNA Circular/genética , Sêmen , Espermatogênese/genética , Células Germinativas , MicroRNAs/genética , Mamíferos/genética
3.
J Environ Manage ; 325(Pt A): 116470, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36244283

RESUMO

Reservoir operation strategies with low cost and high efficiency have been proposed to control algal blooms. However, the key hydrodynamic principle for performing reservoir operation strategies is still unknown, posing an obstacle to practical applications. To address this challenge, we proposed short-term emergency reservoir operation strategies (EROSs), established a three-dimensional (3D) eutrophication model of the Zipingpu Reservoir, and designed six 14-day reservoir operation cases to explore the mechanism of EROSs in controlling algal blooms. Large outflows with rapid water exchange should be adopted early in EROSs to control algal blooms in the reservoir. Small variations in the surface water temperature or the mixed layer depth/euphotic layer depth (Zmix/Zeu) ratio were found for different EROSs, indicating that these variations might not have been responsible for the differences in the algal blooms in the reservoir. The EROSs induced high surface flow velocity (Vs) and depth-averaged velocity (Vd) values in the reservoir, thereby controlling algal blooms by inhibiting algal growth and disrupting algal accumulation in the upper water layers. The flow of Vs against the direction of the water intake was detected during the execution of the EROSs, suggesting that increasing Vs might enhance water retention in the reservoir. Increasing Vd not only promoted water exchange to disrupt algal accumulation but also enhanced Vs to inhibit algal growth. Moreover, Vd demonstrated a strong linear relationship with the inhibition ratio of algal blooms. These results demonstrate that Vd is the key hydrodynamic indicator for performing EROSs and that accelerating Vd to exceed 0.039 m s-1 in the near-dam region can control algal blooms. Overall, in this study, we develop a novel EROS and elucidate corresponding principles for the use of EROSs to control algal blooms in reservoirs.


Assuntos
Eutrofização , Hidrodinâmica , Água , China , Monitoramento Ambiental/métodos
4.
J Exp Med ; 220(1)2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36374225

RESUMO

Within the tumor microenvironment, tumor cells and endothelial cells regulate each other. While tumor cells induce angiogenic responses in endothelial cells, endothelial cells release angiocrine factors, which act on tumor cells and other stromal cells. We report that tumor cell-derived adrenomedullin has a pro-angiogenic as well as a direct tumor-promoting effect, and that endothelium-derived CC chemokine ligand 2 (CCL2) suppresses adrenomedullin-induced tumor cell proliferation. Loss of the endothelial adrenomedullin receptor CALCRL or of the G-protein Gs reduced endothelial proliferation. Surprisingly, tumor cell proliferation was also reduced after endothelial deletion of CALCRL or Gs. We identified CCL2 as a critical angiocrine factor whose formation is inhibited by adrenomedullin. Furthermore, CCL2 inhibited adrenomedullin formation in tumor cells through its receptor CCR2. Consistently, loss of endothelial CCL2 or tumor cell CCR2 normalized the reduced tumor growth seen in mice lacking endothelial CALCRL or Gs. Our findings show tumor-promoting roles of adrenomedullin and identify CCL2 as an angiocrine factor controlling adrenomedullin formation by tumor cells.


Assuntos
Adrenomedulina , Quimiocina CCL2 , Neoplasias , Animais , Camundongos , Adrenomedulina/farmacologia , Proliferação de Células , Quimiocina CCL2/genética , Quimiocinas , Células Endoteliais/patologia , Ligantes , Neoplasias/genética , Neoplasias/patologia , Receptores CCR2/genética , Microambiente Tumoral
5.
Sci Total Environ ; 857(Pt 1): 159367, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36240924

RESUMO

The change of plant biodiversity caused by resource-enhancing global changes has greatly affected grassland productivity. However, it remains unclear how multi-resource enrichment induces the effects of multifaceted biodiversity on grassland productivity under different site resource constraints. We conducted a multiple resource addition (MRA) experiment of water and nutrients at three sites located along a resource gradient in northern China. This allowed us to assess the response of aboveground net primary productivity (ANPP), species (species richness and plant density), functional (functional richness and community-weighted mean of traits) and phylogenetic (phylogenetic richness) diversity to increasing number of MRA. We used structural equation model (SEM) to examine the direct and indirect effects of MRA and multifaceted biodiversity on ANPP. The combined addition of the four resources increased ANPP at all three sites. But with increasing number of MRA, biodiversity varied at the three sites. At the high resource constraint site, species richness, plant density and leaf nitrogen concentration (LNC) increased. At the medium resource constraint site, plant height and LNC increased, leaf dry matter content (LDMC) decreased. At the low resource constraint site, species, functional and phylogenetic richness decreased, and height increased. The SEM showed that MRA increased ANPP directly at all three sites, and indirectly by increasing plant density at the high constraint site and height at the medium constraint site. Independent of MRA, ANPP was affected by height at the high resource constraint site and LNC at the low resource constraint site. Our results illustrate that multi-resource addition positively affects productivity, while affects biodiversity depending on site resource constraint. The study highlights that site resource constraint conditions need to be taken into consideration to better predict grassland structure and function, particularly under the future multifaceted global change scenarios.


Assuntos
Biodiversidade , Pradaria , Filogenia , Biomassa , Plantas , Ecossistema
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 286: 121998, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36279800

RESUMO

Conventional Hg2+ visual sensors are unsustainable, hindering their practical application for improved water quality and health. In order to address this challenge, herein, N, S co-doped carbon nanodots (NS-CDs) were prepared and well characterized, presented the fluorescent monitoring for Hg2+ over other metal ions with the limit of detection (LOD) of 0.47 µM. Next, the CDs were successfully modified by thymine without any fluorescence labelling (referred to as T-NS-CDs). The sensitivity to Hg2+ cloud be noticeable enhanced due to the formation of T-Hg2+-T specific base pairs. Accordingly, the LOD was calculated with values as low as 1.56 nM. Furthermore, Hg2+ could be released and complexed with antidote (meso-2,3-dimercaptosuccinic acid) (DMSA-Hg2+), being the responsible for the reversible interconversion between T-Hg2+-T and DMSA-Hg2+. Interestingly, the proposed sensing system also applies to the fluorescent sensing for Hg2+ in tap water with satisfactory recoveries (96.97 %-101.38 %, RSD < 2 %). Thus, by simply combination of elemental doping and surface functionalization, the surface state and functionalities of CDs could be tailorable, endowing the fluorometric sensing towards Hg2+ in environmental system.


Assuntos
Mercúrio , Pontos Quânticos , Carbono , Nitrogênio , Timina , Antídotos , Enxofre , Espectrometria de Fluorescência , Succímero , Corantes Fluorescentes
7.
Int J Cancer ; 152(1): 90-99, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36111424

RESUMO

Clinically effective methods to predict the efficacy of sunitinib, for patients with metastatic or locally advanced pancreatic neuroendocrine tumors (panNET) are scarce, making precision treatment difficult. This study aimed to develop and validate a computed tomography (CT)-based method to predict the efficacy of sunitinib in patients with panNET. Pretreatment CT images of 171 lesions from 38 patients with panNET were included. CT value ratio (CT value of tumor/CT value of abdominal aorta from the same patient) and radiomics features were extracted for model development. Receiver operating curve (ROC) with area under the curve (AUC) and decision curve analysis (DCA) were used to evaluate the proposed model. Tumor shrinkage of >10% at first follow-up after sunitinib treatment was significantly associated with longer progression-free survival (PFS; P < .001) and was used as the major treatment outcome. The CT value ratio could predict tumor shrinkage with AUC of 0.759 (95% confidence interval [CI], 0.685-0.833). We then developed a radiomics signature, which showed significantly higher AUC in training (0.915; 95% CI, 0.866-0.964) and validation (0.770; 95% CI, 0.584-0.956) sets than CT value ratio. DCA also confirmed the clinical utility of the model. Subgroup analysis showed that this radiomics signature had a high accuracy in predicting tumor shrinkage both for primary and metastatic tumors, and for treatment-naive and pretreated tumors. Survival analysis showed that radiomics signature correlated with PFS (P = .020). The proposed radiomics-based model accurately predicted tumor shrinkage and PFS in patients with panNET receiving sunitinib and may help select patients suitable for sunitinib treatment.


Assuntos
Tumores Neuroendócrinos , Neoplasias Pancreáticas , Humanos , Sunitinibe/uso terapêutico , Tumores Neuroendócrinos/diagnóstico por imagem , Tumores Neuroendócrinos/tratamento farmacológico , Tomografia Computadorizada por Raios X/métodos , Intervalo Livre de Progressão , Estudos Retrospectivos , Neoplasias Pancreáticas/diagnóstico por imagem , Neoplasias Pancreáticas/tratamento farmacológico , Neoplasias Pancreáticas/patologia
8.
Bioact Mater ; 22: 274-290, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36263097

RESUMO

Spinal cord injury (SCI) is a serious clinical disease. Due to the deformability and fragility of the spinal cord, overly rigid hydrogels cannot be used to treat SCI. Hence, we used TPA and Laponite to develop a hydrogel with shear-thinning ability. This hydrogel exhibits good deformation, allowing it to match the physical properties of the spinal cord; additionally, this hydrogel scavenges ROS well, allowing it to inhibit the lipid peroxidation caused by ferroptosis. According to the in vivo studies, the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism. In addition, dental pulp stem cells (DPSCs) were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses. It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI.

9.
MAbs ; 14(1): 2029675, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35133941

RESUMO

The functional interleukin 6 (IL-6) signaling complex is a hexameric structure composed of IL-6, IL-6Rα, and the signaling receptor gp130. There are three different modes of IL-6 signaling, classic signaling, trans-signaling, and trans-presentation, which are not functionally redundant and mediate pleiotropic effects on both physiological and pathophysiological states. Monoclonal antibodies against IL-6 or IL-6Rα have been successfully developed for clinical application. However, designing therapeutic interventions that block specific modes of IL-6 signaling in a pathologically relevant manner remains a great challenge. Here, we constructed a fusion protein Hyper-IL-6 (HyIL-6) composed of human IL-6 and IL-6Rα to develop specific blocking antibodies against the IL-6/IL-6Rα complex. We successfully screened the monoclonal antibody C14mab, which can bind to HyIL-6 with the binding constant 2.86 × 10-10 and significantly inhibit IL-6/IL-6Rα/gp130 complex formation. In vitro, C14mab effectively inhibited HyIL-6-stimulated signal transducer and activator of transcription 3 (STAT3) activation and related vascular endothelial growth factor (VEGF) induction. Moreover, C14mab efficaciously suppressed HyIL-6-induced acute phase response in vivo. Our data from hydrogen-deuterium exchange mass spectrometry demonstrate that C14mab mainly binds to site IIIa of IL-6 and blocks the final step in the interaction between gp130 and IL-6/IL-6Rα complex. Additionally, data from enzyme-linked immunosorbent assays and kinetics assays indicate that C14mab interacts simultaneously with IL-6 and IL-6Rα, while it does not interact with IL-6Rα alone. The unique features of C14mab may offer a novel alternative for IL-6 blockade and illuminate a better therapeutic intervention targeting IL-6.


Assuntos
Interleucina-6 , Receptores de Interleucina-6 , Anticorpos Monoclonais , Receptor gp130 de Citocina/química , Receptor gp130 de Citocina/metabolismo , Epitopos , Humanos , Interleucina-6/metabolismo , Receptores de Interleucina-6/química , Receptores de Interleucina-6/metabolismo , Fator A de Crescimento do Endotélio Vascular
11.
Front Genet ; 13: 1029300, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338997

RESUMO

Computable models as a fundamental candidate for traditional biological experiments have been applied in inferring lncRNA-disease association (LDA) for many years, without time-consuming and laborious limitations. However, sparsity inherently existing in known heterogeneous bio-data is an obstacle to computable models to improve prediction accuracy further. Therefore, a new computational model composed of multiple mechanisms for lncRNA-disease association (MM-LDA) prediction was proposed, based on the fusion of the graph attention network (GAT) and inductive matrix completion (IMC). MM-LDA has two key steps to improve prediction accuracy: first, a multiple-operator aggregation was designed in the n-heads attention mechanism of the GAT. With this step, features of lncRNA nodes and disease nodes were enhanced. Second, IMC was introduced into the enhanced node features obtained in the first step, and then the LDA network was reconstructed to solve the cold start problem when data deficiency of the entire row or column happened in a known association matrix. Our MM-LDA achieved the following progress: first, using the Adam optimizer that adaptively adjusted the model learning rate could increase the convergent speed and not fall into local optima as well. Second, more excellent predictive ability was achieved against other similar models (with an AUC value of 0.9395 and an AUPR value of 0.8057 obtained from 5-fold cross-validation). Third, a 6.45% lower time cost was consumed against the advanced model GAMCLDA. In short, our MM-LDA achieved a more comprehensive prediction performance in terms of prediction accuracy and time cost.

12.
BMC Plant Biol ; 22(1): 514, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36329386

RESUMO

BACKGROUND: Grazing disturbance usually affects floral display and pollination efficiency in the desert steppe, which may cause pollen limitation in insect-pollinated plants. Effective pollination is essential for the reproductive success of insect-pollinated plants and insufficient pollen transfer may result in pollen limitation. Caragana microphylla Lam is an arid region shrub with ecological importance. Few studies have been conducted on how grazing disturbance influences pollen limitation and pollination efficiency of C. microphylla. Here, we quantify the effect of different grazing intensities on floral display, pollinator visitation frequency and seed production in the Urat desert steppe. RESULTS: In C. microphylla, supplemental hand pollination increased the seed set, and pollen limitation was the predominant limiting factor. As the heavy grazing significantly reduced the seed set in plants that underwent open-pollination, but there was no significant difference in the seed set between plants in the control plots and plants in the moderate grazing plots. Furthermore, there was a higher pollinator visitation frequency in plants in the control plots than in plants in the heavy grazing plots. CONCLUSIONS: We found that pollinator visitation frequency was significantly associated with the number of open flowers. Our findings also demonstrated that seed production is associated with pollinator visitation frequency, as indicated by increased seed production in flowers with higher pollinator visitation frequency. Therefore, this study provides insight into the effect of different grazing intensities on floral display that are important for influencing pollinator visitation frequency and pollination efficiency in desert steppes.


Assuntos
Flores , Herbivoria , Insetos , Pólen , Polinização , Animais , Flores/fisiologia , Insetos/fisiologia , Plantas/parasitologia , Polinização/fisiologia , Clima Desértico , Herbivoria/fisiologia
13.
Front Plant Sci ; 13: 1057226, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36426145

RESUMO

The apple is an economically important fruit, and fruit russeting is not conducive to its appearance. Although studies have examined fruit russeting, its mechanism remains unclear. Two apple strains of the F1 hybrid population derived from 'Fuji' and 'Golden Delicious' were used in this study. We found that the skin of russet apples was rough and fissured, while that of non-russet apples was smooth and waxy. Chemical staining, LC- and GC-MS showed that both lignin and suberin were increased in russet apple skin. Meanwhile, genes involved in lignin and suberin synthetic pathways were upregulated in russet apple skin. Additionally, we found many differentially expressed genes (DEGs1) involved in hormone biosynthesis and signaling and stress responses in the two apple strains. We found that WRKY13 may influence russeting by regulating lignin synthesis. Our study identified several candidate metabolites and genes, which will provide a good foundation for further research.

14.
Zhongguo Gu Shang ; 35(11): 1048-52, 2022 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-36415190

RESUMO

OBJECTIVE: To explore the technique of autogenous bone graft combined with plate fixation in total knee arthroplasty(TKA) with severe proximal medial tibial bone defect. METHODS: From March 2012 to October 2018, 21 patients (9 males and 12 females) with severe bone defects in the proximal medial tibia during primary total knee arthroplasty were treated with autogenous structural bone grafting and steel plate fixation, with an age of 61 to 77 years old with an average of (69.6±9.1) years and a course of 64 to 257 months with an average of (73.6±170.7) months. According to Rand classification, there were 13 cases of type Ⅲb and 8 cases of type Ⅳb. Postoperative complications were observed, and knee joint function was evaluated by the Hospital for Special Surgery (HSS) score and SF-36 quality of life score. RESULTS: All 21 patients were followed up for 37 to 64 months with an average of (49.5±13.7) months. The incisions of all patients healed smoothly, and 2 patients developed lower limb intermuscular venous plexus thrombosis after operation. There were no periprosthetic infection, loosening of prosthesis and other complications. The autogenous bone grafts of all patients achieved bony healing during postoperative X-ray follow-up, and the healing time was 8 to 13 months with an average of (10.1±2.3) months. The HSS score of patients increased significantly from 30 to 48 with an average of (53.4±4.2) before operation to 75 to 92 with an average of (81.2±8.4) at the final follow-up (P<0.05). The SF-36 quality of life score of patients after operation was significantly different from that before operation (P<0.05). CONCLUSION: The technique of autogenous bone graft combined with steel plate fixation can achieve satisfactory osseointegration effect in the treatment of severe proximal tibial bone defects in primary knee arthroplasty, with less complications and obvious improvement in knee function.


Assuntos
Artroplastia do Joelho , Masculino , Feminino , Humanos , Pessoa de Meia-Idade , Idoso , Artroplastia do Joelho/métodos , Tíbia/transplante , Transplante Ósseo/métodos , Qualidade de Vida , Transplante Autólogo , Aço
15.
Eur Radiol ; 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36418621

RESUMO

OBJECTIVES: To investigate the efficacy of medical treatment and balloon angioplasty for intracranial atherosclerosis using high-resolution MR vessel wall imaging (HR-MRI). METHODS: In this prospective study, patients with symptomatic severe stenosis from January 2018 to August 2021 were treated with medical treatment or balloon angioplasty. The patients underwent HR-MRI at baseline and at 3, 6, and 12 months. Plaque characteristics at follow-up were compared with those at baseline using paired sample T-test or Wilcoxon rank sum test. The difference in the recurrence of ischemic events between two groups was compared. RESULTS: A total of 37 patients (26 males; mean age = 60.5 ± 11.6 years) were evaluated. Of 68 plaques, 42 (61.8%) were treated with medication only. At 12 months of medical treatment, maximum plaque length (p = 0.004), maximum wall thickness (p = 0.036), and plaque enhancement (p = 0.001) were significantly reduced than baseline. At 3 months after balloon angioplasty, luminal stenosis (p = 0.048) was significantly reduced compared to baseline. At 6 months after balloon angioplasty, maximum plaque length (p = 0.011), maximum wall thickness (p = 0.003), and luminal stenosis (p = 0.001) were significantly reduced than baseline. No difference was found in the recurrence of ischemic events between two groups (p = 0.458). CONCLUSION: Intracranial atherosclerotic plaque shrank and tended to be stable at 12 months of medical treatment. Plaque burden was significantly reduced 6 months after balloon angioplasty. This may provide evidence for the application and selection of treatment strategies for intracranial atherosclerotic disease. KEY POINTS: • Plaque burden and plaque enhancement were significantly reduced at 12 months of medical treatment compared to baseline. • Plaque burden was significantly reduced at 6 months after balloon angioplasty compared with baseline. • No significant difference in the recurrence rate of ischemic stroke between patients treated with medication and balloon angioplasty.

16.
Scand J Clin Lab Invest ; : 1-7, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36421059

RESUMO

BACKGROUND: There is still no consensus on the optimal monitoring method to evaluate the hypothalamic-pituitary-gonadal axis (HPGA) inhibition. METHODS: There were 124 girls treated with triptorelin depot due to puberty disorders, including 77 central precocious puberty and 47 early puberty. After treatment, triptorelin stimulation tests were performed, and blood samples were collected at 0, 20, 40 and 60 min. Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured by immunochemiluminometric assay (ICMA). RESULTS: Peak LH (PLH), peak FSH and estradiol in 124 girls were significantly decreased after treatment, while 2 cases had inadequate treatment efficacy. Areas under the receiver operating characteristic curves (AUC) of PLH and peak FSH after stimulation for the diagnosis of HPGA suppression were 0.984 and 0.121. When the cut-off value of PLH was ≤ 2.25 IU/L, the sensitivity was 96.7% and specificity was 100.0%. There was no difference in AUC between PLH and a single LH at 20, 40, or 60 min (p > 0.05). When LH were ≤ 2.34 IU/L, ≤ 2.21 IU/L and ≤ 2.00 IU/L at 20, 40 and 60 min, respectively, the sensitivity were 99.1%, 96.7% and 98.4%, and the specificity were all 100.0%. The correlation coefficients between PLH and LH at 20, 40 or 60 min were 0.947, 0.975 and 0.961. CONCLUSION: A single blood sample for stimulated LH at 20 min, 40 min, or 60 min assayed by ICMA during triptorelin stimulation test is useful for monitoring the treatment efficacy of triptorelin depot in girls with puberty disorders.

17.
Diabetes ; 71(12): 2664-2676, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36331122

RESUMO

Diabetic kidney disease (DKD) is the leading cause of end-stage kidney disease (ESKD). Prognostic biomarkers reflective of underlying molecular mechanisms are critically needed for effective management of DKD. A three-marker panel was derived from a proteomics analysis of plasma samples by an unbiased machine learning approach from participants (N = 58) in the Clinical Phenotyping and Resource Biobank study. In combination with standard clinical parameters, this panel improved prediction of the composite outcome of ESKD or a 40% decline in glomerular filtration rate. The panel was validated in an independent group (N = 68), who also had kidney transcriptomic profiles. One marker, plasma angiopoietin 2 (ANGPT2), was significantly associated with outcomes in cohorts from the Cardiovascular Health Study (N = 3,183) and the Chinese Cohort Study of Chronic Kidney Disease (N = 210). Glomerular transcriptional angiopoietin/Tie (ANG-TIE) pathway scores, derived from the expression of 154 ANG-TIE signaling mediators, correlated positively with plasma ANGPT2 levels and kidney outcomes. Higher receptor expression in glomeruli and higher ANG-TIE pathway scores in endothelial cells corroborated potential functional effects in the kidney from elevated plasma ANGPT2 levels. Our work suggests that ANGPT2 is a promising prognostic endothelial biomarker with likely functional impact on glomerular pathogenesis in DKD.


Assuntos
Diabetes Mellitus , Nefropatias Diabéticas , Falência Renal Crônica , Humanos , Angiopoietina-1/genética , Receptor TIE-2/genética , Nefropatias Diabéticas/genética , Estudos de Coortes , Células Endoteliais , Angiopoietina-2/genética , Angiopoietinas , Transdução de Sinais , Biomarcadores , Progressão da Doença
18.
Nat Commun ; 13(1): 7097, 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36402785

RESUMO

Flexible sensors, friendly interfaces, and intelligent recognition are important in the research of novel human-computer interaction and the development of smart devices. However, major challenges are still encountered in designing user-centered smart devices with natural, convenient, and efficient interfaces. Inspired by the characteristics of textile-based flexible electronic sensors, in this article, we report a braided electronic cord with a low-cost, and automated fabrication to realize imperceptible, designable, and scalable user interfaces. The braided electronic cord is in a miniaturized form, which is suitable for being integrated with various occasions in life. To achieve high-precision interaction, a multi-feature fusion algorithm is designed to recognize gestures of different positions, different contact areas, and different movements performed on a single braided electronic cord. The recognized action results are fed back to varieties of interactive terminals, which show the diversity of cord forms and applications. Our braided electronic cord with the features of user friendliness, excellent durability and rich interaction mode will greatly promote the development of human-machine integration in the future.


Assuntos
Eletrônica , Têxteis , Humanos , Gestos
19.
Front Microbiol ; 13: 1002691, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36406454

RESUMO

Methicillin-resistant Staphylococcus aureus (MRSA) isolates remain a serious threat to global health despite a decrease in MRSA infections since 2005. MRSA isolates exhibit great diversity worldwide, and their lineages show geographic variation. In this study, we used whole genome sequencing (WGS) to analyze antibiotic resistance genes and virulence genes, spa, staphylococcal cassette chromosome mec, sequence types (STs), and core genome multilocus sequence typing (cgMLST) of MRSA isolates from patients and environmental surface in hospitals in China to determine their prevalence and molecular traits. The highest number of infections by MRSA isolates was observed in patients aged ≥60 years (69.8%, P < 0.05). We identified a total of 19 STs from 162 MRSA isolates from patients. A significant increase was observed in the incidence of ST764-t002-II MRSA infection, which is replacing ST5-t002-II MRSA as the predominant ST. Similarly, isolates from environmental surface were predominantly ST764-t002-II (47%). Notably, most ST764 isolates (97.7%) carried seb, but not arginine catabolic mobile element (ACME), which differed from ST764 isolates in Japan and Thailand. The potential danger of spreading requires rigorous surveillance of emerging ST764 MRSA isolates. We also found higher resistance to seven antimicrobials [OXA, cefoxitin (FOX), ERY, CFZ, ciprofloxacin (CIP), levofloxacin (LEV), and moxifloxacin (MXF)]. Resistance to gentamicin (38.3%), tetracycline (55.9%), and minocycline (41.5%) were also common. Phenotypic resistance to antimicrobials was associated with resistance genes to its content, and cgMLST clustering suggested a strong link between these strains. Overall, our findings revealed the prevalence and molecular characteristics of MRSA isolates in Shanghai, China, providing a theoretical reference for preventing and controlling clonal transmission of MRSA isolates in hospitals in China.

20.
Front Plant Sci ; 13: 1022961, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36407630

RESUMO

The gibberellic acid (GA)-stimulated Arabidopsis (GASA) gene family is highly specific to plants and plays crucial roles in plant growth and development. CcGASA4 is a member of the GASA gene family in citrus plants; however, the current understanding of its function in citrus is limited. We used CcGASA4-overexpression transgenic citrus (OEGA) and control (CON) plants to study the role of CcGASA4 in Shatian pomelo. The RNA sequencing (RNA-seq) analysis showed that 3,522 genes, including 1,578 upregulated and 1,944 downregulated genes, were significantly differentially expressed in the CON versus OEGA groups. The Gene Ontology enrichment analysis showed that 178 of the differentially-expressed genes (DEGs) were associated with flowers. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEGs were enriched in 134 pathways, including "plant-pathogen interaction", "MAPK signaling pathway-plant", "phenylpropane biosynthesis", "plant hormone signal transduction", "phenylalanine, tyrosine and tryptophan biosynthesis", and "flavonoid and flavonol biosynthesis". The most significantly-enriched pathway was "plant-pathogen interaction", in which 203 DEGs were enriched (126 DEGs were upregulated and 78 were downregulated). The metabolome analysis showed that 644 metabolites were detected in the OEGA and CON samples, including 294 differentially-accumulated metabolites (DAMs; 83 upregulated versus 211 downregulated in OEGA compared to CON). The metabolic pathway analysis showed that these DAMs were mainly involved in the metabolic pathways of secondary metabolites, such as phenylpropanoids, phenylalanine, flavone, and flavonol biosynthesis. Thirteen flavonoids and isoflavones were identified as DAMs in OEGA and CON. We also discovered 25 OEGA-specific accumulated metabolites and found 10 that were associated with disease resistance. CcGASA4 may therefore play a functional role in activating the expression of MAPK signaling transduction pathway and disease resistance genes, inhibiting the expression of auxin- and ethylene-related genes, and activating or inhibiting the expression of brassinosteroid biosynthesis- and abscisic acid-related genes. CcGASA4 may also play a role in regulating the composition and abundance of flavonoids, isoflavones, amino acids, purines, and phenolic compounds. This study provides new insights into the molecular mechanisms of action of CcGASA4 in citrus plants.

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