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1.
Rev. bras. med. esporte ; 28(6): 738-740, Nov.-Dec. 2022. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1376772

RESUMO

ABSTRACT Introduction Football is a sport with good acceptance among contemporary university students; it integrates competition, entertainment, and physical fitness. However, few studies demonstrate the concrete advantages found in college players. Objective Explore the impact of football on the physical performance of university students. Methods 100 university volunteers in elementary class 1-3 soccer practitioners, aged 19.2±2.1 years, were evaluated by a battery of tests in body composition, body function, and physical fitness. The results were compared with the literary data, and the experiment went through a mathematical, statistical method to separate the soccer intervention and conduct empirical research on physical health. Results After the 18-week elective course intervention, the 50m and 1000m sprint results changed significantly, especially the 1000m sprint performance greatly improved; the difference was significant (P<0.01). Conclusion Soccer is a sport that improves the physical function and quality of university students and is more conducive to maintaining the physical health of university students. Evidence level II; Therapeutic Studies - Investigating the results.


RESUMO Introdução O futebol é um esporte com boa aceitação entre os universitários contemporâneos, integra a competição, o entretenimento e a aptidão física. Porém há poucos estudos demonstrando as vantagens concretas encontradas nos jogadores universitários. Objetivo Explorar o impacto do futebol no desempenho físico dos universitários. Métodos Um total de 100 universitários voluntários na classe fundamental 1-3 praticantes de futebol, com idade de 19.2±2.1 anos, foram avaliados por bateria de testes em áreas de composição corporal, função corporal e aptidão física. Os resultados foram confrontados com os dados literários, e o experimento passou por método estatístico matemático, para separar a intervenção do futebol e realizar pesquisas empíricas sobre a saúde física. Resultados Após a intervenção de curso eletivo de 18 semanas, os resultados de corrida de 50 metros e 1000 metros mudaram significativamente, especialmente o desempenho da corrida de 1000 metros melhorou muito, a diferença foi muito significativa (P<0,01). Conclusão O futebol é um esporte que melhora a função física e a qualidade dos universitários, além de validar-se como mais propício para a manutenção da saúde física dos universitários. Nível de evidência II; Estudos terapêuticos - Investigação de resultados.


RESUMEN Introducción El fútbol es un deporte con buena aceptación entre los estudiantes universitarios contemporáneos, que integra la competición, el entretenimiento y la forma física. Sin embargo, hay pocos estudios que demuestren las ventajas concretas encontradas en los jugadores universitarios. Objetivo Explorar el impacto del fútbol en el desempeño físico de los estudiantes universitarios. Métodos Un total de 100 voluntarios universitarios en el grado elemental 1-3 que practicaban el fútbol, con una edad de 19,2±2,1 años, fueron evaluados mediante una batería de pruebas en áreas de composición corporal, función corporal y aptitud física. Los resultados se confrontaron con los datos de la literatura, y el experimento pasó por el método estadístico matemático, para separar la intervención del fútbol y realizar una investigación empírica sobre la salud física. Resultados Después de la intervención del curso electivo de 18 semanas, los resultados de la carrera de 50 metros y de 1000 metros cambiaron significativamente, especialmente el rendimiento de la carrera de 1000 metros mejoró mucho, la diferencia fue muy significativa (P<0,01). Conclusión El fútbol es un deporte que mejora la función y la calidad física de los estudiantes universitarios, y se valida como más propicio para mantener la salud física de los estudiantes universitarios. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.


Assuntos
Humanos , Adolescente , Adulto Jovem , Futebol , Estudantes , Composição Corporal , Aptidão Física , Desempenho Físico Funcional
2.
Front Psychol ; 13: 925955, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072024

RESUMO

Autonomy support is one of the most crucial determinants of teaching practice for student engagement. No literature review on the relations between autonomy support and student engagement existed to the best of our knowledge. Therefore, this study presents a systematic literature review from perspectives of landscapes, methodology characters, patterns of identified studies, and autonomy-supportive strategies. Overall, 31 articles were reviewed. Followed by PRISMA guidelines, the results yielded several interesting facts: First, studies on such topics surged starting from 2015 and were mostly conducted in the United States (32%) and Korea (16%). Publications were scattered but heavily gathered around psychological and educational journals such as the Journal of Educational Psychology (9.7%); Learning and Instruction (9.7%). Most often, studies recruited participants from upper secondary schools (58%). Data were collected using solely questionnaires (93.5%) following a two-wave design (51.6%) and were analyzed by applying structural equation models (48.4%). Moreover, most of the studies failed to provide concrete autonomy-supportive teaching strategies. Instead, quite often studies (93%) investigated its relations with student engagement from a macro perspective. Within mentioned strategies, they were mostly related to the teaching process, there is a limited investigation of autonomy-supportive teaching practice used before and after instruction. This pattern of results suggested an urgent need for more longitudinal studies on specific teaching strategies that hold the potential to maximize student engagement. Limitations and suggestions for future studies were provided accordingly.

3.
Plant Commun ; : 100432, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071666

RESUMO

Orychophragmus violaceus is a Brassicaceae species widely cultivated in China, particularly as a winter cover crop in northern China due to its tolerance to low temperature and low demand for water. Recently, O. violaceus has also been cultivated as a potential industrial oil seed crop owing to its abundant 24-carbon dihydroxy fatty acids (diOH-FA), which contribute to superior high-temperature lubricant property. In this study, we conducted the de novo assembly of O. violaceus genome. Further whole-genome synteny analysis with the genomes of its relatives demonstrated that O. violaceus is a diploid undergoing an extra whole-genome duplication (WGD) after the Brassicaceae specific α-WGD event, with a basic chromosome number of x = 12. Notably, the formation of diOH-FA is hypothesized to occur after the WGD event. Based on the genome and the transcriptome data of multistage seed development, we predicted that OvDGAT1-1 and OvDGAT1-2 are the candidate genes regulating the storage of diOH-FA in O. violaceus seeds. These results may greatly facilitate the development of heat-tolerant and eco-friendly plant-based lubricants using O. violaceus seed oil as well as improve our understanding of the genomic evolution of Brassicaceae.

4.
Opt Lett ; 47(17): 4560-4563, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36048704

RESUMO

We propose a novel, to the best of our knowledge, sensor for nanovibration detection based on a microsphere. The sensor consists of a stretched single-mode fiber and a 2 µm microsphere. The light from the optical fiber passes through the microsphere, forming a photonic nanojet (PNJ) phenomenon at the front of the microsphere. The evanescent field in the PNJ enhances the light reflected from the measured object to the single-mode fiber-microsphere probe (SMFMP). Results showed that the system can detect arbitrary nanovibration waveforms in real time with an SMFMP detection resolution of 1 nm. The voltage signal received and the vibration amplitude showed a good linear relationship within the range of 0-100 nm, with a sensitivity of 0.7 mV/nm and a linearity of more than 99%. The sensor is expected to have potential applications in the field of cell nanovibration detection.


Assuntos
Fibras Ópticas , Óptica e Fotônica , Microesferas , Fótons
5.
Mol Biol Rep ; 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36057005

RESUMO

BACKGROUNDS: The narrow genetic basis of cucumber makes breeding of this species difficult. CRISPR/Cas9 system is  characteristic of  simple design, low cost and  high efficiency, which has opened a new path for cucumber functional genetics and the development of cucumber mocular breeding. However, the immature genetic transformation system is the main limiting factor for applying this technology in cucumber. METHODS AND RESULTS: In this study, a Histochemical ß-glucuronidase (GUS) assay was used to analyze the effect of various parameters, including slight scratch of explants, pre-culture time, acetosyringone (AS) concentration, infection time in Agrobacterium solution, and co-culture period on the transformation efficiency. The results showed that the explants slightly scratched after cutting, pre-cultured for 1 day, Agrobacterium bacterial solution containing AS, and 20 min length of infection could significantly increase the GUS staining rate of explants. On this basis, two sequences with high specificity (sgRNA-1 and sgRNA-2) targeted different loci of gene CsGCN5 were designed. The corresponding vectors Cas9-sgRNA-1 and Cas9-sgRNA-2 were constructed and transformed using the above-optimized cucumber genetic transformation system, and three and two PCR positive lines were obtained from 210 and 207 explants, respectively. No sequence mutation at target loci of CsGCN5 was detected in the Cas9-sgRNA-1 transformed three PCR positive lines. However, one mutant line with targeted homozygous change was recognized from the Cas9-sgRNA-2 transformed two PCR positive lines. CONCLUSION: In this study, 2.4‰ of total explants had directed mutation in the CsGCN5 gene. The results in the present study would be beneficial to further optimize and improve the efficiency of the genetic transformation of cucumber.

6.
J Cancer ; 13(10): 3061-3072, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36046639

RESUMO

Chemotherapy is an important treatment for gastric cancer (GC), but the primary and secondary drug resistance of tumours to chemotherapy seriously affects its curative effect. In recent years, the relationship between long noncoding RNAs (lncRNAs) and malignant tumours has received increasing attention. Based on accumulating evidence, lncRNAs are involved in the chemoresistance of GC, but the underlying mechanisms remain unclear. In this study, we identified the lncRNA colorectal neoplasia differentially expressed (CRNDE) as an important regulator of autophagy-associated chemoresistance in GC. Mechanistically, overexpression of CRNDE inhibits autophagy and induces apoptosis, thereby sensitizing GC cells to chemotherapy drugs. Moreover, E2F6, a classical transcriptional inhibitor, is confirmed to be upregulated in GC and represses the expression of CRNDE. The E2F6-CRNDE axis is clinically related to chemoresistant GC and poor outcomes in patients with advanced GC. Our findings suggest that the E2F6-CRNDE axis is a viable therapeutic target to protect against chemoresistance in GC.

7.
HLA ; 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36114742

RESUMO

One nucleotide replacement at nucleotide 397 of HLA-C*07:02:01:01 results in a new allele, HLA-C*07:1024. This article is protected by copyright. All rights reserved.

8.
Protein Pept Lett ; 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36065930

RESUMO

Ciliary neurotrophic factor (CNTF) is a pluripotent neurotrophic factor that was originally isolated from chicken embryo ciliary neurons. It has a powerful role in the development and maintenance of the optic nervous system and has been used for many vision-related diseases. It also plays an important role in the neurogenesis, regeneration and survival of other neurons, including neural stem cells, dorsal root ganglion, sensory neurons and motor neurons. CNTF is related to neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. In addition to its role in nervous system, CNTF regulates the balance of energy metabolism and the administration of CNTF induces body weight loss. More CNTF function have been found with the deepening of study, such as protecting and promoting cardiomyocyte proliferation. In addition, CNTF even participate in the mental illness and inflammation suppressing. CNTF exerts multidirectional physiological activity by regulating the transcription of various genes through a variety of signalling pathways (including JAK/STAT, MAPK, and P13K/AKT). This review summarizes the roles and mechanisms of CNTF in the optic nervous system, retinal-related diseases, neuronal protection, and especially nutrition, energy metabolism and other aspects.

9.
Light Sci Appl ; 11(1): 261, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36055999

RESUMO

Lidar techniques present a distinctive ability to resolve vertical structure of optical properties within the upper water column at both day- and night-time. However, accuracy challenges remain for existing lidar instruments due to the ill-posed nature of elastic backscatter lidar retrievals and multiple scattering. Here we demonstrate the high performance of, to the best of our knowledge, the first shipborne oceanic high-spectral-resolution lidar (HSRL) and illustrate a multiple scattering correction algorithm to rigorously address the above challenges in estimating the depth-resolved diffuse attenuation coefficient Kd and the particulate backscattering coefficient bbp at 532 nm. HSRL data were collected during day- and night-time within the coastal areas of East China Sea and South China Sea, which are connected by the Taiwan Strait. Results include vertical profiles from open ocean waters to moderate turbid waters and first lidar continuous observation of diel vertical distribution of thin layers at a fixed station. The root-mean-square relative differences between the HSRL and coincident in situ measurements are 5.6% and 9.1% for Kd and bbp, respectively, corresponding to an improvement of 2.7-13.5 and 4.9-44.1 times, respectively, with respect to elastic backscatter lidar methods. Shipborne oceanic HSRLs with high performance are expected to be of paramount importance for the construction of 3D map of ocean ecosystem.

10.
Front Genet ; 13: 951252, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36061181

RESUMO

Background: Colorectal cancer (CRC) is the third most prevalent cancer worldwide and the second leading cause of cancer mortality. Signal transducer and activator of transcription (STAT) proteins are a group of transcription factors implicated in cell signal transduction and gene transcription in several cancer types. However, the level of expression, genetic alterations, and biological function of different STATs, as well as their prognostic and immunotherapeutic value in CRC remain unclear. Methods: The mRNA and protein expression levels, genetic alterations, prognostic value, gene-gene and protein-protein interaction networks, and biological function of STATs in CRC were studied using the GEPIA, HPA, cBioPortal, PrognoScan, Kaplan-Meier plotter, GeneMANIA, STRING, and Metascape databases. The expression of STATs in CRC was confirmed using immunohistochemistry (IHC). Finally, the relationship between STAT expression and immune infiltration as well as immunotherapy-associated indicators was also investigated. Results: The expression levels of STAT2/5A/5B are downregulated in CRC, and the STAT1/3/4/5B expressions were significantly associated with the tumor stage of patients with CRC. The abnormal expression of STAT2/4/5B in patients with CRC is related to the prognosis of patients with CRC. The STATs and their neighboring proteins are primarily associated with lymphocyte activation, cytokine-mediated signaling pathways, positive regulation of immune response, regulation of cytokine production, and growth hormone receptor signaling pathways in cancer. The expression of STATs was significantly associated with immune infiltration and immunotherapy response-associated indicators. Conclusion: This study may help further understand the molecular mechanism of CRC and provide new prognostic biomarkers and immunotherapy targets in patients with CRC.

11.
Acta Orthop ; 93: 703-708, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069480

RESUMO

BACKGROUND AND PURPOSE: Identification of pathogens causing fracture-device-related infection (FDRI) is always a challenge as the positive rate of standard tissue sampling culture (TSC) remains unsatisfactory. This study evaluates the efficiency of implant surface culture (ISC) as an adjunct to standard TSC for identification of FDRI-associated microorganisms. PATIENTS AND METHODS: Between November 2020 and March 2022, patients diagnosed with FDRI defined by the International Fracture-Related Infection (FRI) Consensus Group, and indicated for implant removal, underwent both methods for bacteria detection. The test order of ISC and TSC was randomly selected for each patient included, as a within-person randomized design. For ISC, the recovered implants were gently covered with tryptic soy agar after rinsing with normal saline twice, and then incubated at 37℃ 5% CO2 for up to 14 days. For TSC, 5 specimens were sampled and sent to the Clinical Laboratory of Southern Medical University Nanfang Hospital, Guangzhou, for culture and identification. RESULTS: 42 consecutive patients were included, with a mean age of 46 years. The most frequent infection site and implant type were the tibia (21 cases) and plates with screws (30 cases), respectively. Altogether 21 patients were found with positive outcomes by both methods, and the identified pathogens were consistent. ISC found an additional 15 patients showing positive results, which were negative by TSC. Furthermore, the mean culture time of ISC was shorter than that of TSC (1.5 days vs. 3.2 days). INTERPRETATION: ISC may be a useful adjunct to TSC for detection of bacteria causing FDRI, with a relatively higher positive rate and a shorter culture time.


Assuntos
Infecções Bacterianas , Fraturas Ósseas , Remoção de Dispositivo , Fixação Interna de Fraturas , Fraturas Ósseas/cirurgia , Humanos , Pessoa de Meia-Idade , Próteses e Implantes
12.
Fish Shellfish Immunol ; 130: 9-21, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36084886

RESUMO

In addition to eliminating most of the anti-nutritional factors in soybean meal, enzyme-treated soybean meal (ESBM) can also increase the proportion of small peptides. It was found that ESBM can replace fish meal (FM) either partially or completely in diets for some fish and shrimp species. In the present study, the effects of replacing dietary FM with ESBM on growth performance, intestinal microbiota, immunity and mTOR pathway in abalone Haliotis discus hannai (initial weight: 16.75 ± 0.09 g) were investigated after a 100-day feeding trial. Five experimental diets were designed to replace 0%, 25%, 50%, 75% and 100% of dietary FM by ESBM, which were named as ESBM0 (control), ESBM25, ESBM50, ESBM75 and ESBM100, respectively. Results showed that ESBM could replace up to 75% of FM in the diet without significant effect on the weight gain rate (WGR, 118.05%-124.16%) of abalone. The increasing dietary ESBM levels significantly decreased the trypsin activity from 418.52 to 286.52 U/mg protein in the digestive gland. No significant differences in the contents of total cholesterol (T-CHO), ammonia (BLA) and malondialdehyde (MDA) in cell-free hemolymph were observed among the groups with replacement levels of dietary FM by ESBM from 0% to 75%. Excessive replacement level of FM with ESBM (ESBM100) significantly increased the MDA content (2.33 nmol/mg prot.) and pro-inflammatory-related gene expression in digestive gland. Compared with the control group, the mTOR pathway in muscle was significantly upregulated in the ESBM75 group. The digestive gland in the ESBM100 group contained more golden refractile spherules than those in the other groups. The abundance of intestinal microbes such as Halomonas, Zobellella and Bacillus was decreased in the ESBM100 group. In conclusion, up to 75% of replacement of dietary FM by ESBM had no negative effects on the growth performance, intestinal microbiota, immunity and mTOR pathway of abalone.

13.
Redox Biol ; 56: 102468, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36113339

RESUMO

Acute myocardial infarction (MI) triggers oxidative stress, which worsen cardiac function, eventually leads to remodeling and heart failure. Unfortunately, effective therapeutic approaches are lacking. Fibroblast growth factor 7 (FGF7) is proved with respect to its proliferative effects and high expression level during embryonic heart development. However, the regulatory role of FGF7 in cardiovascular disease, especially MI, remains unclear. FGF7 expression was significantly decreased in a mouse model at 7 days after MI. Further experiments suggested that FGF7 alleviated MI-induced cell apoptosis and improved cardiac function. Mechanistic studies revealed that FGF7 attenuated MI by inhibiting oxidative stress. Overexpression of FGF7 actives nuclear factor erythroid 2-related factor 2 (Nrf2) and scavenging of reactive oxygen species (ROS), and thereby improved oxidative stress, mainly controlled by the phosphatidylinositol-3-kinase α (PI3Kα)/AKT signaling pathway. The effects of FGF7 were partly abrogated in Nrf2 deficiency mice. In addition, overexpression of FGF7 promoted hexokinase2 (HXK2) and mitochondrial membrane translocation and suppressed mitochondrial superoxide production to decrease oxidative stress. The role of HXK2 in FGF7-mediated improvement of mitochondrial superoxide production and protection against MI was verified using a HXK2 inhibitor (3-BrPA) and a HXKII VDAC binding domain (HXK2VBD) peptide, which competitively inhibits localization of HXK2 on mitochondria. Furthermore, inhibition of PI3Kα/AKT signaling abolished regulation of Nrf2 and HXK2 by FGF7 upon MI. Together, these results indicate that the cardio protection of FGF7 under MI injury is mostly attributable to its role in maintaining redox homeostasis via Nrf2 and HXK2, which is mediated by PI3Kα/AKT signaling.

14.
Open Med (Wars) ; 17(1): 1425-1437, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36128444

RESUMO

To develop effective medicines, researchers must first understand the common and distinct mechanisms that drive oncogenic processes in human cancers. TWF1 and TWF2 belong to the actin-depolymerizing factor homology family. TWF1 has been identified as an important gene in lung, breast, and pancreatic cancer in recent investigations. TWF2's role in cancer remains largely unknown, no comprehensive pan-cancer studies have been conducted. We utilized the The Cancer Genome Atlas and Gene Expression Omnibus datasets to investigate the role of TWF2 in different types of cancers. TWF2 transcription in cancers and the number of TWF2 mutations were examined as part of our study. We also examined the possible functional pathways involved in TWF2-mediated oncogenicity. Our pan-cancer analysis provided a complete overview of the oncogenic effects of TWF2 in a wide range of human malignancies.

15.
Open Biol ; 12(9): 220179, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36128717

RESUMO

In humans, a single enzyme 2-aminoadipic semialdehyde synthase (AASS) catalyses the initial two critical reactions in the lysine degradation pathway. This enzyme evolved to be a bifunctional enzyme with both lysine-2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase domains (SDH). Moreover, AASS is a unique drug target for inborn errors of metabolism such as glutaric aciduria type 1 that arise from deficiencies downstream in the lysine degradation pathway. While work has been done to elucidate the SDH domain structurally and to develop inhibitors, neither has been done for the LOR domain. Here, we purify and characterize LOR and show that it is activated by alkylation of cysteine 414 by N-ethylmaleimide. We also provide evidence that AASS is rate-limiting upon high lysine exposure of mice. Finally, we present the crystal structure of the human LOR domain. Our combined work should enable future efforts to identify inhibitors of this novel drug target.

16.
Anal Chem ; 2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-36121740

RESUMO

Microfluidic paper-based analytical devices (µPADs) are emerging as powerful analytical platforms in clinical diagnostics, food safety, and environmental protection because of their low cost and favorable substrate properties for biosensing. However, the existing top-down fabrication methods of paper-based chips suffer from low resolution (>200 µm). Additionally, papers have limitations in their physical properties (e.g., thickness, transmittance, and mechanical flexibility). Here, we demonstrate a bottom-up approach for the rapid fabrication of heterogeneously controlled paper-based chip arrays. We simply print a wax-patterned microchip with wettability contrasts, enabling automatic and selective assembly of cellulose microfibers to construct predefined paper-based microchip arrays with controllable thickness. This paper-based microchip printing technology is feasible for various substrate materials ranging from inorganic glass to organic polymers, providing a versatile platform for the full range of applications including transparent devices and flexible health monitoring. Our bottom-up printing technology using cellulose microfibers as the starting material provides a lateral resolution down to 42 ± 3 µm and achieves the narrowest channel barrier down to 33 ± 2 µm. As a proof-of-concept demonstration, a flexible paper-based glucose monitor is built for human health care, requiring only 0.3 µL of sample for testing.

17.
Nat Metab ; 2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-36123394

RESUMO

Adipose tissue undergoes thermogenic remodeling in response to thermal stress and metabolic cues, playing a crucial role in regulating energy expenditure and metabolic homeostasis. Endoplasmic reticulum (ER) stress is associated with adipose dysfunction in obesity and metabolic disease. It remains unclear, however, if ER stress-signaling in adipocytes mechanistically mediates dysregulation of thermogenic fat. Here we show that inositol-requiring enzyme 1α (IRE1α), a key ER stress sensor and signal transducer, acts in both white and beige adipocytes to impede beige fat activation. Ablation of adipocyte IRE1α promotes browning/beiging of subcutaneous white adipose tissue following cold exposure or ß3-adrenergic stimulation. Loss of IRE1α alleviates diet-induced obesity and augments the anti-obesity effect of pharmacologic ß3-adrenergic stimulation. Notably, IRE1α suppresses stimulated lipolysis and degrades Ppargc1a messenger RNA through its RNase activity to downregulate the thermogenic gene program. Hence, blocking IRE1α bears therapeutic potential in unlocking adipocytes' thermogenic capacity to combat obesity and metabolic disorders.

18.
Plant Cell Environ ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071575

RESUMO

Auxin is well known to stimulate coleoptile elongation and rapid seedling growth in the air. However, its role in regulating rice germination and seedling establishment under submergence is largely unknown. Previous studies revealed that excessive levels of indole-3-acetic acid(IAA) frequently cause the inhibition of plant growth and development. In this study, the high-level accumulation of endogenous IAA is observed under dark submergence, stimulating rice coleoptile elongation but limiting the root and primary leaf growth during anaerobic germination (AG). We found that oxygen and light can reduce IAA levels, promote the seedling establishment and enhance rice AG tolerance. miRNA microarray profiling and RNA gel blot analysis results show that the expression of miR167 is negatively regulated by submergence; it subsequently modulates the accumulation of free IAA through the miR167-ARF-GH3 pathway. The OsGH3-8 encodes an IAA-amido synthetase that functions to prevent free IAA accumulation. Reduced miR167 levels or overexpressing OsGH3-8 increase auxin metabolism, reduce endogenous levels of free IAA and enhance rice AG tolerance. Our studies reveal that poor seed germination and seedling growth inhibition resulting from excessive IAA accumulation would cause intolerance to submergence in rice, suggesting that a certain threshold level of auxin is essential for rice AG tolerance.

19.
FASEB J ; 36(10): e22553, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36111980

RESUMO

Mesenchymal stromal cells (MSCs) are attractive candidates for treating hepatic disorders given their potential to enhance liver regeneration and function. The paracrine paradigm may be involved in the mechanism of MSC-based therapy, and exosomes (Exo) play an important role in this paracrine activity. Hypoxia significantly improves the effectiveness of MSC transplantation. However, whether hypoxia preconditioned MSCs (Hp-MSCs) can enhance liver regeneration, and whether this enhancement is mediated by Exo, are unknown. In this study, mouse bone marrow-derived MSCs (BM-MSCs) and secreted Exo were injected through the tail vein. We report that Hp-MSCs promote liver regeneration after partial hepatectomy in mice through their secreted exosomes. Interestingly, MSC-Exo were concentrated in liver 6 h after administration and mainly taken up by macrophages, but not hepatocytes. Compared with normoxic MSC-Exo (N-Exo), hypoxic MSC-Exo (Hp-Exo) enhanced M2 macrophage polarization both in vivo and in vitro. Microarray analysis revealed significant enrichment of microRNA (miR)-182-5p in Hp-Exo compared with that in N-Exo. In addition, miR-182-5p knockdown partially abolished the beneficial effect of Hp-Exo. Finally, Hp-MSC-derived exosomal miR-182-5p inhibited theprotein expression of forkhead box transcription factor 1 (FOXO1) in macrophages, which inhibited toll-like receptor 4 (TLR4) expression and subsequently induced an anti-inflammatory response. These results highlight the therapeutic potential of Hp-Exo in liver regeneration and suggest that miR-182-5p from Hp-Exo facilitates macrophage polarization during liver regeneration by modulating the FOXO1/TLR4 signaling pathway.


Assuntos
Células-Tronco Mesenquimais , MicroRNAs , Animais , Anti-Inflamatórios/farmacologia , Medula Óssea/metabolismo , Proteína Forkhead Box O1/genética , Proteína Forkhead Box O1/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Hipóxia/metabolismo , Regeneração Hepática/genética , Macrófagos/metabolismo , Células-Tronco Mesenquimais/metabolismo , Camundongos , MicroRNAs/metabolismo , Receptor 4 Toll-Like/metabolismo
20.
Org Biomol Chem ; 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36106547

RESUMO

A simple and efficient methodology for the first synthesis of tri- and di-fluoromethyl-bis(indolyl)methanols has been demonstrated through a one-pot Friedel-Crafts-type acylation-alkylation of readily available indoles and fluorinated acids. This simple protocol was successfully performed under metal-, additive-, toxic-solvent-, and protective-gas-free conditions, and delivered a wide range of tri- and di-fluoromethyl-bis(indolyl)methanols in moderate to high yields. Notably, this reaction can tolerate diverse vital and reactive functional groups. Furthermore, this one-pot Friedel-Crafts-type acylation-alkylation can be readily expanded to the gram scale with no obvious decrease in the yield, demonstrating its high application potential.

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