Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.256
Filtrar
1.
J Nanosci Nanotechnol ; 20(3): 1756-1764, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492340

RESUMO

Organic-inorganic CoFe2O4/polyaniline (CoFe2O4/PA) nanocomposites with embedded structures were synthesized by combining the sol-gel auto-combustion process and in-situ oxidative polymerization. The phases and morphologies of the prepared samples were identified. The pure CoFe2O4 samples exhibited inferior microwave-absorption properties in a frequency range of 2-18 GHz. Upon the incorporation of PA, the formed CoFe2O4/PA nanocomposites exhibited rather good absorption performances. When the sample thickness was 2.5 mm, the maximum reflection loss (RL) reached -22.3 dB, while the RL below -10 dB corresponded to the range of 11.0-17.1 GHz, which contains almost the entire Ku-band, making the structure promising for commercial and military applications. A physical model was employed to explain the effects of the embedded structure on the microwave-absorption performances. The excellent microwave-absorption performances could be attributed to the interfacial polarization and repeated reflection of the microwaves inside the CoFe2O4/PA composite.

2.
Mol Cell Biochem ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31602539

RESUMO

Adipose-derived stem cells (ADSCs) and their derivatives have aroused intense interest in fields of dermatological and aesthetic medicine. As a major component detected in ADSCs secretome, platelet-derived growth factor AA (PDGF-AA) has been reported mediating extracellular matrix deposition and remodeling, thus might contribute to its anti-aging effect. On the basis of establishing an experimental model that simulate actual skin aging by exposing HDFs to both intrinsic and extrinsic aging factors, we pretreated human dermal fibroblasts (HDFs) with ADSC-conditioned medium (ADSC-CM) before being irradiated, aiming at exploring preventive effects of ADSCs secretome against aging damages. 48 h after irradiation, we detected cellular proliferation; ß-galactosidase stain; mRNA expressions of MMP-1, MMP-9, and TIMP-1; and protein expressions of collagen I, collagen III, and elastin. Moreover, we detected related protein expression of PI3K/Akt signal pathway, which can be activated by PDGF-AA and was newly found to promote extracellular matrix protein synthesis. Concentration of PDGF-AA in the prepared ADSC-CM decreased over time and maintained excellent bioactivity at low temperature until the 11th week. ADSC-CM pretreatment can slightly or significantly improve cellular proliferative activity and reduce cellular senescence in irradiated HDFs. Besides, ADSC-CM pretreatment increased collagen I, collagen III, elastin, and TIMP-1 expressions but decreased MMP-1 and MMP-9 expressions both in irradiated and nonirradiated HDFs. ADSC-CM pretreatment significantly increased pAkt protein expression, and ECM protein expression greatly decreased in case of LY294002 application. The results were similar in three generations of HDFs, yet varied with different degrees. Generally, ADSC-CM we prepared demonstrates a certain degree of positive role in preventing HDFs from intrinsic and extrinsic aging damages and that PDGF-AA may contribute to making it become effective with some other components in ADSC-CM.

3.
Medicine (Baltimore) ; 98(41): e17387, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31593088

RESUMO

BACKGROUND: Double-lumen bronchial tubes (DLBT) and bronchial blockers (BB) are commonly used in the anesthesia for clinical thoracic surgery. But there are few systematic clinical comparisons between them. In this study, the effects of BB and DLBT on one-lung ventilation (OLV) are studied. METHODS: The 200 patients with thoracic tuberculosis undergoing thoracic surgery, were randomly assigned to group A (DLBT) and group B (BB). Intubation time, hemodynamic changes (mean arterial pressure [MAP], heart rate [HR]), and arterial blood gas indicators (arterial partial pressure of carbon dioxide [PaCO2], arterial partial pressure of oxygen [PaO2], airway plateau pressure [Pplat], and airway peak pressure [Ppeak]) at 4 time points were recorded. Complications such as hoarseness, pulmonary infection, pharyngalgia, and surgical success rate were also evaluated postoperatively. RESULTS: Intubation times were shorter in group B. Both MAP and HR in group A were significantly higher 1 minute after intubation than before, but also higher than those in group B. PaO2 levels were lower in both groups during (OLV) than immediately after anesthesia and after two-lung ventilation (TLV), with PaO2 being lower after 60 minutes of OLV than after 20 minutes of OLV. Furthermore, at both points during OLV, PaO2 was lower in group A than in group B. No significant differences in PaCO2 were found between the 2 groups. Ppeak and Pplat were increased in both groups during OLV, with both being higher in group A than in group B. The incidence of postoperative hoarseness, pulmonary infection, and pharyngalgia were lower in group B. There was no significant difference in the success rate of operation between the 2 groups. CONCLUSIONS: Compare with using DLBT, implementation of BB in general anesthesia has less impact on hemodynamics, PaO2 and airway pressures, and achieves lower incidence of postoperative complication.

4.
Medicine (Baltimore) ; 98(39): e17354, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574878

RESUMO

INTRODUCTION: The incidence, mortality, and treatment costs of sepsis are high and, thus, present a major challenge for critical care medicine. Our previous studies suggest that intestinal metabolite granisetron has a potential therapeutic effect on sepsis. Granisetron is a clinically widely used antiemetic, which is safe, inexpensive, and reliable. However, its value in the treatment of sepsis remains unclear. This study aims to explore the efficacy and safety of granisetron in the treatment of sepsis. METHODS AND ANALYSIS: A single-center, single-blind, randomized, controlled clinical trial will be conducted on 154 patients with sepsis. Patients who meet sepsis 3.0 diagnostic criteria, aged ≥18 and ≤80 years, with PCT ≥ 2 ng/mL will be recruited. Patients will be randomized to receive intravenous granisetron 3 mg every 8 hours (n = 77) or an equal volume of normal saline (n = 77) for a treatment period of 4 days or to ICU discharge. The primary outcome is 28-day all-cause mortality. Secondary outcome measures include requirements for organ function support, changes of organ function, changes in infection biomarkers, changes in inflammatory and immune biomarkers, and the proportion of new organ failure. Adverse events and serious adverse events also will be observed closely. ETHICS AND DISSEMINATION: The study was approved by the Clinical Ethics Committee of Zhujiang Hospital of Southern Medical University (2018-ZZJHZX-009). The trial results will be disseminated at national and international conferences and through peer-reviewed journal. TRIAL REGISTRATION: NCT03924518.URL: www.clinicaltrials.gov. PROTOCOL DATE: 1 May 2019. version 2.1.


Assuntos
Granisetron/administração & dosagem , Sepse/tratamento farmacológico , Antagonistas da Serotonina/administração & dosagem , Choque Séptico/tratamento farmacológico , Administração Intravenosa , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como Assunto , Sepse/mortalidade , Choque Séptico/mortalidade , Método Simples-Cego , Resultado do Tratamento , Adulto Jovem
5.
Adv Mater ; : e1903665, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566267

RESUMO

Planar optical elements that can manipulate the multidimensional physical parameters of light efficiently and compactly are highly sought after in modern optics and nanophotonics. In recent years, the geometric phase, induced by the photonic spin-orbit interaction, has attracted extensive attention for planar optics due to its powerful beam shaping capability. The geometric phase can usually be generated via inhomogeneous anisotropic materials, among which liquid crystals (LCs) have been a focus. Their pronounced optical properties and controllable and stimuli-responsive self-assembly behavior introduce new possibilities for LCs beyond traditional panel displays. Recent advances in LC-mediated geometric phase planar optics are briefly reviewed. First, several recently developed photopatterning techniques are presented, enabling the accurate fabrication of complicated LC microstructures. Subsequently, nematic LC-based transmissive planar optical elements and chiral LC-based broadband reflective elements are reviewed systematically. Versatile functionalities are revealed, from conventional beam steering and focusing, to advanced structuring. Combining the geometric phase with structured LC materials offers a satisfactory platform for planar optics with desired functionalities and drastically extends exceptional applications of ordered soft matter. Some prospects on this rapidly advancing field are also provided.

6.
Adv Mater ; : e1905161, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566274

RESUMO

Currently, n-type acceptors in high-performance all-polymer solar cells (all-PSCs) are dominated by imide-functionalized polymers, which typically show medium bandgap. Herein, a novel narrow-bandgap polymer, poly(5,6-dicyano-2,1,3-benzothiadiazole-alt-indacenodithiophene) (DCNBT-IDT), based on dicyanobenzothiadiazole without an imide group is reported. The strong electron-withdrawing cyano functionality enables DCNBT-IDT with n-type character and, more importantly, alleviates the steric hindrance associated with typical imide groups. Compared to the benchmark poly(naphthalene diimide-alt-bithiophene) (N2200), DCNBT-IDT shows a narrower bandgap (1.43 eV) with a much higher absorption coefficient (6.15 × 104 cm-1 ). Such properties are elusive for polymer acceptors to date, eradicating the drawbacks inherited in N2200 and other high-performance polymer acceptors. When blended with a wide-bandgap polymer donor, the DCNBT-IDT-based all-PSCs achieve a remarkable power conversion efficiency of 8.32% with a small energy loss of 0.53 eV and a photoresponse of up to 870 nm. Such efficiency greatly outperforms those of N2200 (6.13%) and the naphthalene diimide (NDI)-based analog NDI-IDT (2.19%). This work breaks the long-standing bottlenecks limiting materials innovation of n-type polymers, which paves a new avenue for developing polymer acceptors with improved optoelectronic properties and heralds a brighter future of all-PSCs.

7.
J Am Chem Soc ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31580665

RESUMO

Temporal and reversible control over protein and cell conjugations holds great potential for traceless release of antibody-drug conjugates (ADCs) on tumor sites as well as on-demand altering or removal of targeting elements on cell surface. We herein developed a bioorthogonal and traceless releasable reaction on proteins and intact cells to fulfill such purposes. Sys-tematic survey of transition metals in catalyzing the bioorthogonal cleavage reactions revealed that copper complexes such as Cu(I)-BTTAA and dual-substituted propargyl (dsPra) or propargyloxycarbonyl (dsProc) moieties offered a bioorthogonal releasable pair for reversible blockage and rescue of primary amines and phenol alcohols on small molecule drugs, protein side-chains as well as intact cell surface. For proof-of-concept, we employed such Cu(I)-BTTAA/dsProc and Cu(I)-BTTAA/dsPra pairs as a "traceless linker" strategy to construct cleavable ADCs to unleash cytotoxic compounds on cancer cells in situ, and as a "reversible modification" strategy for cell surface engineering. Furthermore, by coupling with the genet-ic code expansion strategy, we site-specifically modulated ligand-receptor interactions on live cell membrane. Together, our work expanded the transition metal-mediated bioorthogonal cleavage toolkit from terminal decaging to internal-linker breakage, which offered a temporal and reversible conjugation strategy on therapeutic proteins and cells.

8.
Biochem Biophys Res Commun ; 519(1): 113-120, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31474334

RESUMO

Gastric cancer (GC) is still a major lethal gastrointestinal tumor. In this study, we clarified that RAB13, which is a member of Rab GTPase family and responsible for cargos delivery between the Golgi and the plasma membrane, plays critical roles in the proliferation and the chemotherapeutic resistance in GC cells. Analyzing RAB13 expression in GC specimens, we found that its mRNA level was higher in cancerous tissues compared with normal counterparts and this increase was further associated with malignant progression of GC. Moreover, increased RAB13 indicated poor overall survival (OS) and progression free survival (PFS) in GC patients. We then found that deletion of RAB13 inhibited the proliferation and promoted the apoptosis in AGS and NCI-N87 cells, the impairments of viability which was due to reduced amount of RAB13 anchoring the plasma membrane and attenuated cellular response to EGF treatment and the activation of downstream Akt/ERK/mTOR signaling pathways accordingly. Moreover, in vitro experiments showed that RAB13 deletion enhanced the sensitization of AGS and NCI-N87 cells toward cisplatin (CDDP) and 5-fluorouracil (5-FU) treatment respectively. Together, these data demonstrate that RAB13 promotes the proliferation and confers CDDP and 5-FU resistance to GC cells, which provides experimental support to target this protein in future clinical practice.

9.
Mar Drugs ; 17(10)2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547564

RESUMO

Alginase lyase is an important enzyme for the preparation of alginate oligosaccharides (AOS), that possess special biological activities and is widely used in various fields, such as medicine, food, and chemical industry. In this study, a novel bifunctional alginate lyase (AlgH) belonging to the PL7 family was screened and characterized. The AlgH exhibited the highest activity at 45 °C and pH 10.0, and was an alkaline enzyme that was stable at pH 6.0-10.0. The enzyme showed no significant dependence on metal ions, and exhibited unchanged activity at high concentration of NaCl. To determine the function of non-catalytic domains in the multi-domain enzyme, the recombinant AlgH-I containing only the catalysis domain and AlgH-II containing the catalysis domain and the carbohydrate binding module (CBM) domain were constructed and characterized. The results showed that the activity and thermostability of the reconstructed enzymes were significantly improved by deletion of the F5/8 type C domain. On the other hand, the substrate specificity and the mode of action of the reconstructed enzymes showed no change. Alginate could be completely degraded by the full-length and modified enzymes, and the main end-products were alginate disaccharide, trisaccharide, and tetrasaccharide. Due to the thermo and pH-stability, salt-tolerance, and bifunctionality, the modified alginate lyase was a robust enzyme which could be applied in industrial production of AOS.

10.
Neurosurgery ; 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31557290

RESUMO

BACKGROUND: Though the Pipeline Embolization Device (Medtronic) is approved for use in adults 22 yr and older, the high efficacy and long-term durability of the device is attractive for treatment of intracranial aneurysms in younger patients who often have aneurysms less amenable to traditional endovascular treatments. OBJECTIVE: To report technical, angiographic, and clinical outcomes in patients aged 21 or below undergoing flow-diversion treatment for intracranial aneurysms. METHODS: Retrospective review across 16 institutions identified 39 patients aged 21 or below undergoing 46 treatment sessions with Pipeline Embolization Device placement between 2012 and 2018. A total of 50 intracranial aneurysms were treated. Details regarding patient demographics, aneurysm characteristics, treatment considerations, clinical outcomes, and aneurysm occlusion were obtained and analyzed in a multicenter database. RESULTS: A total of 70% of patients were male. Nonsaccular morphology was seen in half of identified aneurysms. Six aneurysms were giant, and five patients were treated acutely after ruptured presentation. Eight patients were younger than 10 yr of age. Complete aneurysm occlusion was seen in 74% of treated aneurysms. Three aneurysms (6%) were retreated. A total of 83% of patients had a modified Rankin Scale scores of ≤2 at last clinical follow-up. There were 2 early mortalities (4.3%) in the immediate postprocedure period because of rerupture of a treated ruptured aneurysm. No recanalization of a previously occluded aneurysm was observed. CONCLUSION: Flow-diversion treatment is a safe and effective treatment for intracranial aneurysms in patients younger than 22 yr. Rates of complete aneurysm occlusion and adverse events are comparable for rates seen in older patients.

11.
Sensors (Basel) ; 19(19)2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31557958

RESUMO

Plant leaf diseases are closely related to people's daily life. Due to the wide variety of diseases, it is not only time-consuming and labor-intensive to identify and classify diseases by artificial eyes, but also easy to be misidentified with having a high error rate. Therefore, we proposed a deep learning-based method to identify and classify plant leaf diseases. The proposed method can take the advantages of the neural network to extract the characteristics of diseased parts, and thus to classify target disease areas. To address the issues of long training convergence time and too-large model parameters, the traditional convolutional neural network was improved by combining a structure of inception module, a squeeze-and-excitation (SE) module and a global pooling layer to identify diseases. Through the Inception structure, the feature data of the convolutional layer were fused in multi-scales to improve the accuracy on the leaf disease dataset. Finally, the global average pooling layer was used instead of the fully connected layer to reduce the number of model parameters. Compared with some traditional convolutional neural networks, our model yielded better performance and achieved an accuracy of 91.7% on the test data set. At the same time, the number of model parameters and training time have also been greatly reduced. The experimental classification on plant leaf diseases indicated that our method is feasible and effective.

12.
Aging (Albany NY) ; 11(18): 7339-7356, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31527304

RESUMO

Aging is a complex physiological phenomenon associated with oxidative stress damage. The objective of this study was to investigate the potential effects of leonurine on D-galactose-induced aging in mice and its possible mechanisms. In this study, we first tested the antioxidant activity of leonurine in vitro. A subcutaneous injection of D-galactose in mice for 8 weeks was used to establish the aging model to evaluate the protective effects of leonurine. The results showed that treatment with 150 mg·kg-1 leonurine could improve the mental condition, organic index, and behavioural impairment; significantly increase the activities of antioxidative enzymes including SOD, CAT, and T-AOC; and ameliorate the advanced glycation end product (AGE) level and histopathological injury. Furthermore, the Western blotting data revealed that leonurine supplementation noticeably modulated the suppression of the Nrf2 pathway and upregulated the downstream expression of HO-1 and NOQ1 in aging mice. Additionally, leonurine treatment activated Nrf2 nuclear translocation in both aging mice and normal young mice, and the expression levels of Nrf2 in normal young mice was higher than those in naturally aging mice. In conclusion, our findings suggest that leonurine is a promising agent for attenuating the aging process, and the underlying molecular mechanisms depend on activating the Nrf2 pathway.

13.
Ann Transplant ; 24: 532-540, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31527567

RESUMO

BACKGROUND Glenoid labrum injury of the shoulder commonly occurs in athletes, especially those who perform throwing motions. This study investigated the effects of the established allogenic tendon-autologous cartilage cells reconstruction approach in a rabbit model of glenoid labrum damage. MATERIAL AND METHODS The allogenic tendons were isolated and extracted using the chemical extraction method. Cartilage cells were isolated from New Zealand rabbits and identified by detecting type II collagenase. The allogenic tendon-autologous cartilage cells were transplanted to the damaged glenoid labrum. HE staining was used to observe inflammatory cells, Masson staining was used to observe muscle fibers, and scanning electron microscopy (SEM) was used to assess antigenicity of tendon tissues. PSA and AB staining were used to examine neutral protein mucopolysaccharide and acidic protein mucopolysaccharide, respectively. We assessed cartilage cell growth in autologous cartilage cells combined with allogenic tendon transplanted tissues. RESULTS Allogenic tendons were well prepared using chemical extraction method due to use of HE staining, Masson staining, and SEM. TGF-ß1 treatment induced cartilage cell formation and triggered expression of acidic and neutral protein mucopolysaccharides. HE staining, Masson staining, PAS staining, and AB staining methods showed that autologous cartilage cells combined with allogenic tendon transplanted tissues had better growth of cartilage cells. CONCLUSIONS This study establishes the allogenic tendon-autologous cartilage cells reconstruction and transplantation approach and illustrated higher adhesive ability and growth ability, and better chondrogenesis in a rabbit model of glenoid labrum damage.

14.
Heart Rhythm ; 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31521807

RESUMO

BACKGROUND: Sudden cardiac death due to malignant arrhythmias is a common cause of death in dilated cardiomyopathy (DCM). Whether genetic variants increase the risk of arrhythmias in DCM is unknown. OBJECTIVES: To investigate the genetic causes of arrhythmias in DCM patients. METHODS: Whole-exome sequencing (WES) and high-depth targeted next-generation sequencing (142-gene panel) were utilized. Eight specific DCM pedigrees with arrhythmias and two separate cohorts of 1232 consecutive unrelated sporadic DCM patients from three medical centers (550 in the discovery cohort; 682 in the replication cohort) were analyzed; 470 (250 in the discovery cohort; 220 in the replication cohort) suffered from arrhythmias (DCM-A group) and 762 (300 in the discovery cohort; 462 in the replication cohort) did not (DCM-NA group). All identified causative variants were Sanger sequenced to eliminate false-positive results and then screened in 700 unrelated matched arrhythmia- and DCM-free healthy controls. RESULTS: We identified LQTS-causative variants that independently cosegregated in two unrelated DCM-LQTS pedigrees. Pathogenic variants in arrhythmia-related genes (Ion-channelopathies) were identified in 4.9% (23/470) of sporadic DCM-A patients (4.0% in the discovery cohort and 5.9% in the replication cohort) but only 0.1% (1/762) of sporadic DCM-NA patients (P=2.16 × 109). These arrhythmia-related pathogenic variants included long QT syndrome, atrial fibrillation, sick sinus syndrome, cardiac conduction disease, and Brugada syndrome. CONCLUSIONS: Some arrhythmias in DCM patients are caused by arrhythmia-related pathogenic variants. For DCM patients with explicit arrhythmias, arrhythmia-causative genetic screening may help to explain the etiology and decision making.

15.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 33(10): 1270-1274, 2019 Oct 15.
Artigo em Chinês | MEDLINE | ID: mdl-31544438

RESUMO

Objective: To analyze the failure factors of proximal femoral nail antirotation (PFNA) in the treatment of geriatric intertrochanteric fractures. Methods: The clinical data of 136 cases of intertrochanteric fracture treated with PFNA internal fixation between May 2015 and June 2017 were retrospectively analyzed. There were 106 males and 30 females, aged from 60 to 80 years, with an average age of 75.5 years. According to Evans-Jensen classification, there were 45 cases of type Ⅰ, 50 cases of type Ⅱ, 23 cases of type Ⅲ, 13 cases of type Ⅳ, and 5 cases of type Ⅴ. The time from injury to operation was 2-4 days, with an average of 3 days. According to the X-ray films before and after operation, the fracture types (stable and unstable), reduction quality (according to Baumgaertner's criteria), integrity of lateral wall and posteromedial cortex of the patients with failure of PFNA internal fixation were summarized, and the causes of failure were analyzed. Results: All the 136 patients were followed up 7-18 months (mean, 13.6 months). There were 17 cases (12.5%) of PFNA internal fixation failure after operation, including 3 cases of stable fracture and 14 cases of unstable fracture; the quality of fracture reduction was excellent in 2 cases, good in 5 cases, and poor in 10 cases; 10 cases with complete lateral wall and 7 cases with defect; 9 cases with complete posteromedial cortex and 8 cases with defect. Reasons for failure of internal fixation: ① There were 8 cases of coxa varus at 12 weeks after operation due to the loss of posteromedial cortex of femoral intertrochanteric, 7 of them continued non-weight-bearing observation and fracture healed at 6 months after operation; 1 case underwent total hip arthroplasty with spiral blade excision after operation. ② There were 7 cases of internal fixation failure caused by lateral wall defect, including 2 cases of screw blade retraction, continued non-weight-bearing observation, and removed the internal fixator after fracture healing; 2 cases of malunion of rotation with the rotation of no more than 15°, fracture healed at 6 months after operation without special treatment; and 3 cases of rupture of lateral intertrochanteric wall during operation, continued non-weight-bearing observation and fracture healed at 6 months after operation. ③ The distal locking of the main screw was deviated in 2 cases during operation. One of them was found and replanted in time during operation, and 1 case was found with fracture of femoral shaft on 3 days after operation, following 1 year of non-weight-bearing observation and fracture ending healing. Conclusion: The types of intertrochanteric fractures (especially unstable fractures), the integrity of the proximal lateral wall of femur, and the defect of the posteromedial cortex are the internal risk factors for the success or failure of PFNA in the treatment of geriatric intertrochanteric fractures. The effective protection of the lateral wall during operation and the good quality of fracture reduction are the external factors that must be paid attention to.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31514149

RESUMO

Biological targets are most commonly proteins such as enzymes, ion channels, and receptors. They are anything within a living organism to bind with some other entities (like an endogenous ligand or a drug), resulting in change in their behaviors or functions. Exploring potential drug-target interactions (DTIs) are crucial for drug discovery and effective drug development. Computational methods were widely applied in drug-target interactions, since experimental methods are extremely time-consuming and resource-intensive. In this paper, we proposed a novel deep learning-based prediction system, with a new negative instance generation, to identify DTIs. As a result, our method achieved an accuracy of 0.9800 on our created dataset. Another dataset derived from DrugBank was used to further assess the generalization of the model, which yielded a good performance with accuracy of 0.8814 and AUC value of 0.9527 on the dataset. The outcome of our experimental results indicated that the proposed method, involving the credible negative generation, can be employed to discriminate the interactions between drugs and targets.

17.
Nat Microbiol ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31477895

RESUMO

The special organelle-located MAVS, STING and TLR3 are important for clearing viral infections. Although TLR4 triggers NF-κB activation to produce pro-inflammatory cytokines for bacterial clearance, effectors with special organelle localization have not been identified. Here, we screened more than 280 E3 ubiquitin ligases and discovered that the endoplasmic reticulum-located Hrd1 regulates TLR4-induced inflammation during bacterial infection. Hrd1 interacts directly with the deubiquitinating enzyme Usp15. Unlike the classical function of Hrd1 in endoplasmic reticulum-associated degradation, Usp15 is not degraded but loses its deubiquitinating activity for IκBα deubiquitination, resulting in excessive NF-κB activation. Importantly, Hrd1 deficiency in macrophages protects mice against lipopolysaccharide-induced septic shock, and knockdown of Usp15 in Hrd1-knockout macrophages restores the reduced IL-6 production. This study proposes that there is crosstalk between Hrd1 and TLR4, thereby linking the endoplasmic reticulum-plasma membrane function during bacterial infection.

18.
Sensors (Basel) ; 19(17)2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31484441

RESUMO

Passive multiple sound source localization is a challenging problem in underwater acoustics, especially for a short hydrophone array in the deep ocean. Several attempts have been made to solve this problem by applying compressive sensing (CS) techniques. In this study, one greedy algorithm in CS theory combined with a spatial filter was developed and applied to a two-source localization scenario in the deep ocean. This method facilitates localization by utilizing the greedy algorithm with a spatial filter at several iterative loops. The simulated and experimental data suggest that the proposed method provides a certain localization performance improvement over the use of the Bartlett processor and the greedy algorithm without a spatial filter. Additionally, the effects on the source localization caused by factors such as the array aperture, number of hydrophones or snapshots, and signal-to-noise ratio (SNR) are demonstrated.

19.
Appl Opt ; 58(25): 6854-6864, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31503656

RESUMO

Optical interferometric techniques provide noncontact, full-field, and high-precision measurements that are very attractive in various research and application fields. Single fringe-pattern processing (SFPP) is often required when measuring fast phenomena, which contain multiple steps including noise removal, phase demodulation, and unwrapping. However, several difficulties are encountered during SFPP, among which the processing time is of interest due to the increasing computational load brought by the large amount and high-resolution fringe patterns in recent years. In this paper, we propose a general and complete graphics processing unit (GPU)-based SFPP framework to perform a systematic discussion on SFPP acceleration. Typical methods from the spatial domain, the transform-based, and the path-related are chosen to have a variety of methods in the framework for better parallelization demonstration, namely, coherence-enhancing diffusion for denoising, spiral phase quadrature transform for demodulation, and quality-guided phase unwrapping. To the best of our knowledge, this is the first time a complete GPU-based framework has been proposed for SFPP. The advantages of performing the analysis and parallelization in framework level are demonstrated, where processing redundancy can be identified and reduced. The proposed framework can be used as an example to demonstrate the GPU-based parallelization in SFPP. Methods in the framework can be replaced but the framework level analysis, the parallel design, and the involved functions are always good references. Experiments are performed on simulated and experimental fringe patterns to demonstrate the effectiveness of the proposed work and achieve at most 29.8 times speedup compared with CPU-based sequential processing.

20.
Nat Commun ; 10(1): 4087, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31501430

RESUMO

Untethered small actuators have various applications in multiple fields. However, existing small-scale actuators are very limited in their intractability with their surroundings, respond to only a single type of stimulus and are unable to achieve programmable structural changes under different stimuli. Here, we present a multiresponsive patternable actuator that can respond to humidity, temperature and light, via programmable structural changes. This capability is uniquely achieved by a fast and facile method that was used to fabricate a smart actuator with precise patterning on a graphene oxide film by hydrogel microstamping. The programmable actuator can mimic the claw of a hawk to grab a block, crawl like an inchworm, and twine around and grab the rachis of a flower based on their geometry. Similar to the large- and small-scale robots that are used to study locomotion mechanics, these small-scale actuators can be employed to study movement and biological and living organisms.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA