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1.
Sci Total Environ ; 698: 134236, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31493577

RESUMO

The widespread emergence of antibiotic resistance genes (ARGs) in drinking water systems endangers human health, and may be exacerbated by their horizontal gene transfer (HGT) among microbiota. In our previous study, Quorum sensing (QS) molecules produced by bacteria from biological activated carbon (BAC) biofilms were demonstrated to influence the transfer efficiency of a model conjugative plasmid, here RP4. In this study, we further explored the effect and mechanism of QS on conjugation transfer. The results revealed that Acyl-homoserine lactones producing (AHL-producing) bacteria isolated from BAC biofilm play a role in the propagation of ARGs. We selected several quorum sensing inhibitors (QSIs) to study their effects on AHL-producing bacteria, including the formation of biofilm and the regulating effect on conjugation transfer. In addition, the possible molecular mechanisms for AHLs that promote conjugative transfer were attributable to enhancing the mRNA expression, which involved altered expressions of conjugation-related genes. We also found that QSIs could inhibit conjugative transfer by downregulating the conjugation-relevant genes. We believe that this is the first insightful exploration of the mechanism by which AHLs will facilitate and QSIs will inhibit the conjugative transfer of ARGs. These results provide creative insight into ARG pollution control that involves blocking QS during BAC treatment in drinking water systems.

2.
Cell Signal ; 65: 109457, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31676369

RESUMO

Esophageal squamous cell carcinoma (ESCC) presents with lymph node metastasis in the early stages, limiting the opportunities for curative local resection, including endoscopic submucosal dissection (ESD). ESD is regarded as the standard treatment for early-stage ESCCs. However, radical surgery is recommended when lymph node metastasis risk exists. More efforts are needed to find the markers for early prediction and clarify the molecular mechanism underlying the pathogenesis of lymph node metastasis. Recently, aberrant regulation of gene expression by histone methylation modifiers has emerged as an important mechanism for cancer metastasis. Herein, we demonstrated that mixed-lineage leukemia 2 (MLL2) positively regulates gene expression programs associated with ESCC cell migration. MLL2 interacts with RelA in the nucleus to enhance transcription of stanniocalcin-1 (STC1) and to facilitate cancer metastasis. Meanwhile, MLL2 knockdown resulted in a significant decrease in the migration of ESCC cells. Clinically, high level of MLL2 was significantly associated with early-stage ESCC lymph node metastasis. In summary, these findings discovered a previously unidentified molecular pathway underlying the coordinated regulation of metastasis-related STC-1 expression by MLL2 and RelA and highlighted the critical role of MLL2 in ESCC.

3.
Chemosphere ; 239: 124748, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31520971

RESUMO

A total of 100 samples were collected from the sediments of a typical copper mining area, south China. Leaching concentrations of selected heavy metals (Ni, Cd, Cu, Pb, Zn, Ba, As, and Hg) were measured to evaluate their distribution patterns and associated health risk. Leaching concentrations of Cu (3.58 ±â€¯1.49 mg/L), Pb (1.50 ±â€¯1.06 mg/L), and Zn (4.04 ±â€¯1.68 mg/L) were significantly higher than the other metals in the samples. By evaluating the spatial heterogeneity, it was found that leaching metal concentrations did not decrease with environmental gradients, mostly caused by diverse distribution in pollution sources. The hazard index and carcinogenic risk indices showed significant risks of human exposure. For public safety, priority governance should be given to the main pollutants (Cd, Cu, Pb, Zn, and As) in sediments. In future studies, the integrated data will be urgently required for local stakeholders to conduct environmental monitoring and remediation scenarios.

4.
J Nanosci Nanotechnol ; 20(5): 3195-3200, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635664

RESUMO

Surface-enhanced fluorescence detection has large potential for detecting many chemical and biological trace analytes. This paper presents a novel method for preparing silver nanomaterials in microfluidic chip channels for the surface-enhanced fluorescence detection of fluorescent dye (SYBR Green I) molecules. Microfluidic chip channels were fabricated by a 248-nm excimer laser. Silver nanoparticles (Ag-NPs) were prepared inside the microfluidic chip channels by directly heating the silver precursor solution. The influence of different temperatures on the sizes of the silver nanoparticles was studied. Then, the surface-enhanced fluorescence technology based on the microfluidic system was used to detect the fluorescent dye molecules. As a result, the fluorescence signal of the fluorescent dye molecules was significantly enhanced by the silver nanoparticles. In addition, the effect of particle size on the fluorescence signal was studied. This simple and fast method is suitable for a fluorescent PCR (polymerase chain reaction) system and has good application prospects for detecting harmful microorganisms in a spacecraft.

5.
Sensors (Basel) ; 19(21)2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31671917

RESUMO

In this paper, we propose a novel direction-of-arrival (DOA) estimation structure based on multiple-input multiple-output (MIMO) radar with colocated antennas, referred to as compressive measurement-based MIMO (CM-MIMO) radar, where the compressive sensing (CS) is employed to reduce the number of channels. Therefore, the system complexity and the computational burden are effectively reduced. It is noted that CS is used after the matched filters and that a measurement matrix with less rows than columns is multiplied with the received signals. As a result, the configurations of the transmit and receive antenna arrays are not affected by the CS and can be determined according to the practical requirements. To study the estimation performance, the Cramér-Rao bound (CRB) with respect to the DOAs of the proposed CM-MIMO radar is analyzed in this paper. The derived CRB expression is also suitable for the conventional MIMO radar by setting the measurement matrix as an identity matrix. Moreover, the CRB expression can work in the under-determined case, since the sum-difference coarray structure is considered. However, the random measurement matrix leads to high information loss, thus compromising the estimation performance. To overcome this problem, we consider that the a prior probability distribution of the DOAs associated with the targets can be obtained in many scenarios and an optimization approach for the measurement matrix is proposed in this paper, where the maximum mutual information criterion is adopted. The superiority of the proposed structure is validated by numerical simulations.

6.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(10): 1292-1294, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31771732

RESUMO

OBJECTIVE: Inflammation and infection are main causes of death in critically ill patients. But traditional treatment are non-comprehensive and limited. Human CCAAT/enhancer binding protein epsilon (C/EBPε) is a key transcription factor regulating the terminal differentiation of neutrophils. It plays an important role in anti-inflammatory and anti-infective process by regulating inflammatory response cells. This article reviews the changes of C/EBPε in inflammation and infection, related regulatory mechanisms and targeted reversal measures, in order to provide references for exploring new directions and effective measures for inflammation and infection treatment.

7.
J Neuroinflammation ; 16(1): 214, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722723

RESUMO

BACKGROUND: Neonatal hypoxic-ischemic brain damage (HIBD), a leading cause of neonatal mortality, has intractable sequela such as epilepsy that seriously affected the life quality of HIBD survivors. We have previously shown that ion channel dysfunction in the central nervous system played an important role in the process of HIBD-induced epilepsy. Therefore, we continued to validate the underlying mechanisms of TRPV1 as a potential target for epilepsy. METHODS: Neonatal hypoxic ischemia and oxygen-glucose deprivation (OGD) were used to simulate HIBD in vivo and in vitro. Primarily cultured astrocytes were used to assess the expression of TRPV1, glial fibrillary acidic protein (GFAP), cytoskeletal rearrangement, and inflammatory cytokines by using Western blot, q-PCR, and immunofluorescence. Furthermore, brain electrical activity in freely moving mice was recorded by electroencephalography (EEG). TRPV1 current and neuronal excitability were detected by whole-cell patch clamp. RESULTS: Astrocytic TRPV1 translocated to the membrane after OGD. Mechanistically, astrocytic TRPV1 activation increased the inflow of Ca2+, which promoted G-actin polymerized to F-actin, thus promoted astrocyte migration after OGD. Moreover, astrocytic TRPV1 deficiency decreased the production and release of pro-inflammatory cytokines (TNF, IL-6, IL-1ß, and iNOS) after OGD. It could also dramatically attenuate neuronal excitability after OGD and brain electrical activity in HIBD mice. Behavioral testing for seizures after HIBD revealed that TRPV1 knockout mice demonstrated prolonged onset latency, shortened duration, and decreased seizure severity when compared with wild-type mice. CONCLUSIONS: Collectively, TRPV1 promoted astrocyte migration thus helped the infiltration of pro-inflammatory cytokines (TNF, IL-1ß, IL-6, and iNOS) from astrocytes into the vicinity of neurons to promote epilepsy. Our study provides a strong rationale for astrocytic TRPV1 to be a therapeutic target for anti-epileptogenesis after HIBD.

8.
BMC Genomics ; 20(1): 846, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722674

RESUMO

BACKGROUND: Recent studies have shown that long non-coding RNAs (lncRNAs) play a crucial role in the induction of cancer through epigenetic regulation, transcriptional regulation, post-transcriptional regulation and other aspects, thus participating in various biological processes such as cell proliferation, differentiation and apoptosis. As a new nova of anti-tumor therapy, immunotherapy has been shown to be effective in many tumors of which PD-1/PD-L1 monoclonal antibodies has been proofed to increase overall survival rate in advanced gastric cancer (GC). Microsatellite instability (MSI) was known as a biomarker of response to PD-1/PD-L1 monoclonal antibodies therapy. The aim of this study was to identify lncRNAs signatures able to classify MSI status and create a predictive model associated with MSI for GC patients. METHODS: Using the data of Stomach adenocarcinoma from The Cancer Genome Atlas (TCGA), we developed and validated a lncRNAs model for automatic MSI classification using a machine learning technology - support vector machine (SVM). The C-index was adopted to evaluate its accuracy. The prognostic values of overall survival (OS) and disease-free survival (DFS) were also assessed in this model. RESULTS: Using the SVM, a lncRNAs model was established consisting of 16 lncRNA features. In the training cohort with 94 GC patients, accuracy was confirmed with AUC 0.976 (95% CI, 0.952 to 0.999). Veracity was also confirmed in the validation cohort (40 GC patients) with AUC 0.950 (0.889 to 0.999). High predicted score was correlated with better DFS in the patients with stage I-III and lower OS with stage I-IV. CONCLUSION: This study identify 16 LncRNAs signatures able to classify MSI status. The correlation between lncRNAs and MSI status indicates the potential roles of lncRNAs interacting in immunotherapy for GC patients. The pathway of these lncRNAs which might be a target in PD-1/PD-L1 immunotherapy are needed to be further study.

9.
J Colloid Interface Sci ; 560: 237-246, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31670021

RESUMO

A fiber asymmetric supercapacitor system is designed with NiCo-LDH nanoflakes and FeOOH nanosheets anchored on electrochemically activated graphite fibers as positive electrode and negative electrode, respectively. Due to the formation of COMetal bonding, the oxygen-functionalized carbon on electrochemically activated graphite fibers can bind strongly with NiCo-LDH and FeOOH, which assists in establishing the fast electron transfer routes and fluent ion transport avenues. Both NiCo-LDH and FeOOH anchored on electrochemically activated graphite fibers display a high rate performance, 80% and 87.3% of the electric capacity can be reserved with the current density increasing from 2 to 20 A g-1 and 2 to 10 A g-1, respectively, while the NiCo-LDH and FeOOH deposited on untreated graphite fibers can only retain 45% and 40%. The fabricated novel solid-state fiber asymmetric supercapacitor device exhibits an expanded operation potential window of 1.8 V with a maximum energy density (130 W h kg-1) when the power density is 1.8 kW kg-1. Furthermore, a high energy density (81 W h kg-1) is still achieved at a superhigh power density (10.8 kW kg-1). Additionally, a good cycling stability of the solid-state fiber asymmetric supercapacitor can be obtained (90% capacity retention after 10,000 cycles).

10.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(4): 346-350, 2019 Jul 28.
Artigo em Chinês | MEDLINE | ID: mdl-31701720

RESUMO

OBJECTIVE: To investigate the molecular mechanism of trichloroethylene (TCE) cardiac developmental toxicity on zebrafish embryos and to try to provide experimental data for related intervention. METHODS: Zebrafish embryos were purchased from the National Zebrafish Resource Center. The embryos were divided into DMSO(control group), DMSO+CHIR, DMSO+XAV, TCE, TCE+CHIR and TCE+XAV groups(TCE at the concentration of 1, 10 and 100 ppb, with the DMSO as control; DMSO: Dimethyl suldoxide; CHIR: CHIR-99021, Wnt agonist; XAV: XAV-939, Wnt antagonist), 60 embryos per group. Zebrafish embryos were fed in systematic aquaculture water, 28℃. The water was replaced every 24 h and drugs were added according to the grouping scheme. The cardiac tissues were dissected and analyzed by transcriptome microarray after RNA extraction. The expressions of Wnt signaling pathway related genes were verified by q-PCR. Wnt atagonist XAV and activator CHIR were used alone or in combination to further evaluate the possibility of the Wnt signaling participating in the cardiac developmental toxicity induced by TCE. RESULTS: Compared with control, Zebra fish embryos exposed to TCE showed a significant increase in heart defects, and the main phenotypes were abnormal atrioventricular ratio, looping defects and pericardial edema. The results of microarray profiling showed that the expressions of genes related to Wnt signaling pathway were affected significantly. The results of qPCR further confirmed that TCE inhibited the expressions of Wnt pathway target genes Axin2, Sox9b and Nkx2.5(P<0.05). Wnt agonist CHIR reduced the TCE-induced cardiac malformation rate significantly, while the addition of Wnt antagonist XAV markedly enhanced the cardiac developmental toxicity of TCE. CONCLUSION: Exposure to TCE leads to heart malformation in zebrafish embryos. Wnt signaling pathway may be involved in the cardiac developmental toxicity induced by TCE.


Assuntos
Coração/efeitos dos fármacos , Coração/embriologia , Tricloroetileno/efeitos adversos , Via de Sinalização Wnt/efeitos dos fármacos , Peixe-Zebra , Animais , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Transcriptoma
11.
J Cell Mol Med ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31746143

RESUMO

Glioma is a common brain malignancy for which new drug development is urgently needed because of radiotherapy and drug resistance. Recent studies have demonstrated that artemisinin (ARS) compounds can display antiglioma activity, but the mechanisms are poorly understood. Using cell lines and mouse models, we investigated the effects of the most soluble ARS analogue artesunate (ART) on glioma cell growth, migration, distant seeding and senescence and elucidated the underlying mechanisms. Artemisinin effectively inhibited glioma cell growth, migration and distant seeding. Further investigation of the mechanisms showed that ART can influence glioma cell metabolism by affecting the nuclear localization of SREBP2 (sterol regulatory element-binding protein 2) and the expression of its target gene HMGCR (3-hydroxy-3-methylglutaryl coenzyme A reductase), the rate-limiting enzyme of the mevalonate (MVA) pathway. Moreover, ART affected the interaction between SREBP2 and P53 and restored the expression of P21 in cells expressing wild-type P53, thus playing a key role in cell senescence induction. In conclusion, our study demonstrated the new therapeutic potential of ART in glioma cells and showed the novel anticancer mechanisms of ARS compounds of regulating MVA metabolism and cell senescence.

13.
Hum Brain Mapp ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31680376

RESUMO

Migraine is a chronic neurological disorder characterized by attacks of moderate or severe headache accompanying functionally and structurally maladaptive changes in brain. As the headache days/month is often measured by patient self-report and tends to be overestimated than actually experienced, the possibility of using neuroimaging data to predict migraine attack frequency is of great interest. To identify neuroimaging features that could objectively evaluate patients' headache days, a total of 179 migraineurs were recruited from two data center with one dataset used as the training/test cohort and the other used as the validating cohort. The guidelines for controlled trials of prophylactic treatment of chronic migraine in adults were used to identify the frequency of attacks and migraineurs were divided into low (MOl) and high (MOh) subgroups. Whole-brain functional connectivity was used to build multivariate logistic regression models with model iteration optimization to identify MOl and MOh. The best model accurately discriminated MOh from MOl with AUC of 0.91 (95%CI [0.86, 0.95]) in the training/test cohort and 0.79 in the validating cohort. The discriminative features were mainly located within the limbic lobe, frontal lobe, and temporal lobe. Permutation tests analysis demonstrated that the classification performance of these features was significantly better than chance. Furthermore, the indicator of functional connectivity had a higher odds ratio than behavioral variables with implementing a holistic regression analysis. The current findings suggested that the migraine attack frequency could be distinguished by using machine-learning algorithms, and highlighted the role of brain functional connectivity in revealing underlying migraine-related neurobiology.

14.
Biosci Biotechnol Biochem ; : 1-9, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31680642

RESUMO

Previous studies have shown that miR-210-3p is involved in the development and progression of atherosclerosis, but its specific mechanisms are still unclear. This study aims to reveal the mechanism of miR-210-3p and its target genes in macrophage lipid deposition and inflammatory response, and provide new ideas for the treatment of atherosclerosis. We found miR-210-3p increased sharply in the first 12 h induced by higher doses of ox-LDL in THP-1 macrophages and then gradually decreased. MiR-210-3p mimic transfection inhibited lipid uptake and inflammatory cytokine production in ox-LDL-induced macrophages. By inhibiting IGF2/IGF2R, miR-210-3p suppressed the expression of fatty acid transcriptase CD36 and transcription factor NF-κB in ox-LDL-induced macrophages. In conclusion, miR-210-3p inhibits the expression of CD36 and NF-κB by inhibiting IGF2 / IGF2R, thereby reducing lipid accumulation and inflammatory response in ox-LDL-induced macrophages. Enhancing miR-210-3p expression may be a new strategy for the treatment of atherosclerosis.

15.
Endokrynol Pol ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31681973

RESUMO

Failed cannulation in the right adrenal vein, which makes the sampling results in the contralateral vein and inferior vena cava (IVC) nonsense, is the main obstacle of using adrenal vein sampling (AVS) in the lateralization diagnosis in primary aldosteronism (PA). We performed a retrospective study to evaluate the specificity and sensitivity of using Aldosterone index (AI) in PA lateralization diagnosis. We enrolled 116 patients who were diagnosed with PA and then underwent AVS in West China Hospital of Sichuan University from April 2015 to April 2017. AI, calculated by dividing the aldosterone concentration of the failed side by the aldosterone concentration of IVC, was used for lateralization diagnosis if the cannulation was judged failed by traditional method. Patients with dominant adrenal gland based on successful AVS were included in subgroup 2 (N=75), while the patients diagnosed with a dominant gland using AI method were enrolled in subgroup 1(N=41). No significant difference of clinical and biochemical findings between the two groups was detected (p value after operation > 0.05). ROC analysis was performed to test the specificity and sensitivity based on AI in subgroup 2. The AUC for dominant gland detection was 0.76, which resulted in 91.3% sensitivity and 67.53% specificity. The positive and negative likelihood ratios were 2.81. Our data suggested that the modified strategy using AI to diagnose the dominant gland in PA is an efficient method when cannulation failed in the right side.

16.
World J Gastroenterol ; 25(40): 6077-6093, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31686764

RESUMO

BACKGROUND: Central sensitization plays a pivotal role in the maintenance of chronic pain induced by chronic pancreatitis (CP). We hypothesized that the nucleus tractus solitarius (NTS), a primary central site that integrates pancreatic afferents apart from the thoracic spinal dorsal horn, plays a key role in the pathogenesis of visceral hypersensitivity in a rat model of CP. AIM: To investigate the role of the NTS in the visceral hypersensitivity induced by chronic pancreatitis. METHODS: CP was induced by the intraductal injection of trinitrobenzene sulfonic acid (TNBS) in rats. Pancreatic hyperalgesia was assessed by referred somatic pain via von Frey filament assay. Neural activation of the NTS was indicated by immunohistochemical staining for Fos. Basic synaptic transmission within the NTS was assessed by electrophysiological recordings. Expression of vesicular glutamate transporters (VGluTs), N-methyl-D-aspartate receptor subtype 2B (NR2B), and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subtype 1 (GluR1) was analyzed by immunoblotting. Membrane insertion of NR2B and GluR1 was evaluated by electron microscopy. The regulatory role of the NTS in visceral hypersensitivity was detected via pharmacological approach and chemogenetics in CP rats. RESULTS: TNBS treatment significantly increased the number of Fos-expressing neurons within the caudal NTS. The excitatory synaptic transmission was substantially potentiated within the caudal NTS in CP rats (frequency: 5.87 ± 1.12 Hz in CP rats vs 2.55 ± 0.44 Hz in sham rats, P < 0.01; amplitude: 19.60 ± 1.39 pA in CP rats vs 14.71 ± 1.07 pA in sham rats; P < 0.01). CP rats showed upregulated expression of VGluT2, and increased phosphorylation and postsynaptic trafficking of NR2B and GluR1 within the caudal NTS. Blocking excitatory synaptic transmission via the AMPAR antagonist CNQX and the NMDAR antagonist AP-5 microinjection reversed visceral hypersensitivity in CP rats (abdominal withdraw threshold: 7.00 ± 1.02 g in CNQX group, 8.00 ± 0.81 g in AP-5 group and 1.10 ± 0.27 g in saline group, P < 0.001). Inhibiting the excitability of NTS neurons via chemogenetics also significantly attenuated pancreatic hyperalgesia (abdominal withdraw threshold: 13.67 ± 2.55 g in Gi group, 2.00 ± 1.37 g in Gq group, and 2.36 ± 0.67 g in mCherry group, P < 0.01). CONCLUSION: Our findings suggest that enhanced excitatory transmission within the caudal NTS contributes to pancreatic pain and emphasize the NTS as a pivotal hub for the processing of pancreatic afferents, which provide novel insights into the central sensitization of painful CP.

17.
J Hazard Mater ; : 121521, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31699484

RESUMO

Trichloroethylene (TCE), a widely used chlorinated solvent, is a common environmental pollutant. Current evidence shows that TCE could induce heart defects during embryonic development, but the underlining mechanism(s) remain unclear. Since activation of the aryl hydrocarbon receptor (AHR) could induce oxidative stress, we hypothesized that AHR-mediated oxidative stress may play a role in the cardiac developmental toxicity of TCE. In this study, we found that the reactive oxygen species (ROS) scavenger, N-Acetyl-L-cysteine (NAC), and AHR inhibitors, CH223191 (CH) and StemRegenin 1, significantly counteracted the TCE-induced heart malformations in zebrafish embryos. Moreover, both CH and NAC suppressed TCE-induced ROS and 8-OHdG (8-hydroxy-2' -deoxyguanosine). TCE did not affect ahr2 and cyp1a expression, but increased cyp1b1 expression, which was restored by CH supplementation. CH also attenuated the TCE-induced mRNA expression changes of Nrf2 signalling genes (nrf2b, gstp2, sod2, ho1, nqo1) and cardiac differentiation genes (gata4, hand2, c-fos, sox9b). In addition, the TCE enhanced SOD activity was attenuated by CH. Morpholino knockdown confirmed that AHR mediated the TCE-induced ROS and 8-OHdG generation in the heart of zebrafish embryos. In conclusion, our results suggest that AHR mediates TCE-induced oxidative stress, leading to DNA damage and heart malformations in zebrafish embryos.

18.
Psych J ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722451

RESUMO

Involuntary mental time travel (MTT) refers to the phenomenon of mentally reliving past experiences or pre-living possible future events in an involuntary form. Few studies have explored involuntary MTT in individuals with schizotypal personality features. The present study aimed to first explore the psychometric properties of the Involuntary Autobiographic Memory Inventory (IAMI) in a Chinese sample (Study 1), and then to explore whether individuals with schizotypal personality features experience involuntary MTT more frequently than individuals without schizotypal features. Moreover, the study explored whether the aberrant frequency of involuntary MTT is correlated with positive schizotypal features (Study 2). The results showed that the IAMI had good structural validity and reliability in a Chinese sample. Individuals with schizotypal traits reported a significantly higher frequency, less positive emotion, and stronger emotional intensity for both involuntary memories and future thoughts compared with individuals without schizotypal features. Further analyses in individuals with schizotypal personality features showed that the frequencies of both involuntary memories and future thoughts were significantly correlated with positive schizotypal traits. These results have potential theoretical and clinical implications for a comprehensive understanding of involuntary MTT among individuals with schizotypal personality features.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31722788

RESUMO

BACKGROUND: Adrenal venous sampling (AVS) is the gold standard for preoperative differentiation between unilateral and bilateral primary aldosteronism (PA). However, results are sometimes vitiated by failing to access the right adrenal vein. MATERIALS AND METHODS: The present study assumed that clinical decisions can be made with incomplete AVS data, by comparing aldosterone/cortisol (A/C) ratio in both left and right adrenal veins with that in the inferior vena cava (LAV/IVC and RAV/IVC). Receiver operation characteristic (ROC) curve and scatterplot were used to certify the upper and lower cutoffs and to analyze the significance of discrimination. One hundred and sixty patients diagnosed with PA from April 2017 to June 2018 underwent AVS in the Urology Department of West China Hospital, Chengdu, China. One hundred and eleven with complete AVS data were divided into 3 groups: left-sided (N=40), right-sided (N=29) and bilateral (N=42). We also collected patients from September 2018 to April 2019 in our department as validation cohort to test our hypothesis. RESULTS: On the basis of LAV/IVC, RAV/IVC and diagnostic category, upper cutoff was 1.14 (50% sensitivity and 100% specificity) and lower cutoff 0.07 (27.5% sensitivity and 100% specificity) for LAV/IVC, and 1.04 (55% sensitivity and 100% specificity) and 0.08 (40% sensitivity and 100% specificity), respectively, for RAV/IVC. CONCLUSION: The diagnostic model in this study contributes to clinical decision-making in patients with only partial PA with incomplete AVS data.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31742383

RESUMO

Inspired by the highly importance of hollow structures in nature such as blood vessels and bamboos in matter transportation, properties enhancement or even survival of living creatures, the creation of hollow materials remains considerable interest. However, constructing hollow unique living-like soft and wet polymeric hydrogels with desirable structures and functionalities is still a big challenge. Here, we reported a robust and effective strategy to fabricate complex three-dimensional hollow polymeric hydrogel with designed shape and function diversity on the basis of supramolecular interactions. Through placing a Ca2+ included gelatin core into the solution of alginate, hydrogel shell could be formed along the shape of the gelatin core via coordination between alginate chains and Ca2+ diffused from gelatin. Hollow hydrogel could finally be obtained by dissolving the gelatin core. Various complex 3D hollow structures could be achieved by designing/constructing assembled gelatin core as building block with adjustable supramolecular metal coordination position and strength. Moreover, hollow hydrogels with function diversity could be developed by introducing functional polymers or nanoparticles into the hydrogel wall. This work has made important progress in developing hollow polymeric hydrogel with desirable structures, shapes and various functional applications including soft actuators and chemical reaction containers.

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