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1.
Chemosphere ; 287(Pt 4): 132414, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34600010

RESUMO

BACKGROUND: Numerous epidemiological findings have shown that di-(2-ethylhexyl)-phthalate (DEHP), one of industrial plasticizers with endocrine-disrupting properties, positively contributes to high incidence of obesity. However, potential pathogenesis of dietary DEHP exposure-induced obesity remains largely unknown. METHODS: Chronic DEHP exposure at different doses (0.05 and 5 mg/kg body weight) to mice had been continuously lasted for 14 weeks through the diet. A combination of targeted quantitative metabolomics (LC/GC-MS) with global 1H NMR-based metabolic profiling to explore the effects of dietary DEHP exposure with different doses on host lipid metabolism of mice. Metagenomics (16S rRNA gene sequencing) was also employed to examine the alterations of gut microbiota composition in the cecal contents of mice after dietary DEHP exposure. RESULTS: Dietary exposure to DEHP at both doses induced weight gain and hepatic lipogenesis of mice by promoting the uptake of fatty acids and disrupting phospholipids and choline metabolism. Dietary DEHP exposure altered the gut microbiota community with disruption of intestinal morphology and reduction of Firmicutes to Bacteroidetes ratio in the cecal contents of mice. Furthermore, DEHP exposure activated gut microbiota fermentation process producing excess short chain fatty acids of mice. CONCLUSION: These findings provide systematic evidence that long-term chronic DEHP exposure induces obesity through disruption of host lipid metabolism and gut microbiota in mice, which not only confirm the epidemiological results, but also expand our understanding of metabolic diseases caused by environmental pollutants exposure.


Assuntos
Dietilexilftalato , Microbioma Gastrointestinal , Animais , Dietilexilftalato/toxicidade , Metabolismo dos Lipídeos , Camundongos , Obesidade/induzido quimicamente , Ácidos Ftálicos , RNA Ribossômico 16S/metabolismo
2.
J Pharm Biomed Anal ; 207: 114400, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34624818

RESUMO

A sensitive and ratiometric electrochemical biosensor was developed for the determination of dimethoate via alkaline phosphatase (ALP) mediated dissolution of nano-MnO2 and [Ru(NH3)6]3+(Ru(III)) redox recycling. The electroactive probe Ru(III) was adsorbed on the nano-MnO2 with the high specific surface area through electrostatic interaction to form the MnO2-Ru(III) nanocomposite, which was then fixed on the surface of the glassy carbon electrode. When the dimethoate inhibited the catalytic activity of ALP in a homogeneous system, the hydrolysate L-ascorbic acid (AA) produced by ALP hydrolysis of L-ascorbic acid-trisodium 2-phosphate (AAP) decreased. The solution was then incubated with a glassy carbon electrode modified by MnO2-Ru(III). At this time, only a small amount of MnO2-Ru(III) was decomposed and Ru(III) was rapidly electroreduced to Ru(II) on the surface of the electrode. The in-situ produced Ru(II) was chemically oxidized back to Ru(III) by Fe(III). The redox recycling of Ru(III) was completed and the Ru(III) reduction current signal was amplified. The process consumed part of Fe(III) to reduce the reduction current signal of Fe(III), and the ratio of the two reduction currents (IRu(III)/IFe(III)) increased significantly. The IRu(III)/IFe(III) value increased with the increase of dimethoate concentration in the linear range of 0.01-300 ng mL-1, and the detection limit was 6.3 pg mL-1. It has been successfully applied to the determination of dimethoate in oilseed rape and lettuce with a satisfactory result.


Assuntos
Fosfatase Alcalina , Técnicas Biossensoriais , Dimetoato , Compostos Férricos , Compostos de Manganês , Oxirredução , Óxidos , Solubilidade
3.
Appl Opt ; 60(32): 10132-10139, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34807120

RESUMO

In order to improve the performance and simplify the structure of an optical antenna for a space laser communication system, we design a free-form off-axis three-mirror optical antenna with an integrated primary/tertiary-mirror structure. The adoption of the integrated primary/tertiary-mirror structure improves efficiency of light energy utilization and reduces the complexity of optical processing and assembly. The introduction of free-form optical elements and optical structure constraints helps to correct the off-axis aberration and realize a large field of view. The obtained optical antenna has the magnification of five times and field of view of 2.4∘×2.4∘. The image quality obtained here reaches the diffraction-limited level. At the communication wavelength of 808 nm, the wavefront error is better than λ/22, and the system has a high energy concentration. The proposed optical antenna could not only improve tracking accuracy of the optical antenna in space but also greatly reduce the complexity of the laser communication system. It has reference significance and application value for free-space laser communication.

4.
Neuroreport ; 32(18): 1428-1435, 2021 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-34776505

RESUMO

OBJECTIVE: Neural projections from the ventral hippocampal CA1 (vCA1) to the medial prefrontal cortex (mPFC) and from the posterior dorsal hippocampal CA1 (pdCA1) to the mPFC are important for information processing. Previous studies have revealed that two forms of long-term synaptic plasticity, long-term potentiation (LTP) and long-term depression (LTD), and γ oscillations, which are all believed to be neural bases for learning and memory in the vCA1-mPFC and the pdCA1-mPFC pathways, exhibited distinct profiles. However, the underlying mechanism is unclear. METHODS: In the present study, amperometric cholinergic signals were monitored during in-vivo electrophysiological recordings of evoked extracellular postsynaptic potentials and spontaneous local field potentials. RESULTS: The results demonstrated that in the vCA1-mPFC pathway, increased cholinergic currents and γ power of the amperometric cholinergic signals were accompanied by the induction of LTD and increased γ power of local field potentials, whereas, in the pdCA1-mPFC pathway, decreased cholinergic currents and increased γ power of the amperometric cholinergic signals were accompanied by the induction of LTP and increased γ power of local field potentials. CONCLUSION: These findings indicated that some correlations may exist between amperometric cholinergic signals and synaptic plasticity in the hippocampus-mPFC pathway. The pattern of alterations in cholinergic signals may help to illustrate the specific properties of synaptic plasticity and γ oscillations in the vCA1-mPFC and the pdCA1-mPFC pathways.

5.
Front Oncol ; 11: 754843, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34820327

RESUMO

Purpose: To develop and internally validate a nomogram combining radiomics signature of primary tumor and fibroglandular tissue (FGT) based on pharmacokinetic dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and clinical factors for preoperative prediction of sentinel lymph node (SLN) status in breast cancer patients. Methods: This study retrospectively enrolled 186 breast cancer patients who underwent pretreatment pharmacokinetic DCE-MRI with positive (n = 93) and negative (n = 93) SLN. Logistic regression models and radiomics signatures of tumor and FGT were constructed after feature extraction and selection. The radiomics signatures were further combined with independent predictors of clinical factors for constructing a combined model. Prediction performance was assessed by receiver operating characteristic (ROC), calibration, and decision curve analysis. The areas under the ROC curve (AUCs) of models were corrected by 1,000-times bootstrapping method and compared by Delong's test. The added value of each independent model or their combinations was also assessed by net reclassification improvement (NRI) and integrated discrimination improvement (IDI) indices. This report referred to the "Transparent Reporting of a multivariable prediction model for Individual Prognosis Or Diagnosis" (TRIPOD) statement. Results: The AUCs of the tumor radiomic model (eight features) and the FGT radiomic model (three features) were 0.783 (95% confidence interval [CI], 0.717-0.849) and 0.680 (95% CI, 0.604-0.757), respectively. A higher AUC of 0.799 (95% CI, 0.737-0.862) was obtained by combining tumor and FGT radiomics signatures. By further combining tumor and FGT radiomics signatures with progesterone receptor (PR) status, a nomogram was developed and showed better discriminative ability for SLN status [AUC 0.839 (95% CI, 0.783-0.895)]. The IDI and NRI indices also showed significant improvement when combining tumor, FGT, and PR compared with each independent model or a combination of any two of them (all p < 0.05). Conclusion: FGT and clinical factors improved the prediction performance of SLN status in breast cancer. A nomogram integrating the DCE-MRI radiomics signature of tumor and FGT and PR expression achieved good performance for the prediction of SLN status, which provides a potential biomarker for clinical treatment decision-making.

6.
Nanotechnology ; 33(8)2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34781277

RESUMO

The supercapacitors possessing high energy storage and long serving period have strategic significance to solve the energy crisis issues. Herein, fluffy nano-dendrite structured cobalt phosphide (CoP) is grown on carbon cloth through simple hydrothermal and electrodeposition treatments (CoP/C-HE). Benefit from its excellent electrical conductivity and special structure, CoP/C-HE manifests a high specific capacity of 461.4 C g-1at 1 A g-1. Meanwhile, the capacity retention remains 92.8% over 10 000 cycles at 5 A g-1, proving the superior cycling stability. The phase conversion of Co2P during the activation process also contributes to the improved performance. The assembled two-electrode asymmetric supercapacitor demonstrates excellent performance in terms of energy density (42.4 W h kg-1at a power density of 800.0 W kg-1) and cycling stability (86.3% retention over 5000 cycles at 5 A g-1), which is superior to many reported cobalt-based supercapacitors. Our work promotes the potential of transition metal phosphides for the applications in supercapacitors.

7.
Dis Markers ; 2021: 5866267, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34840631

RESUMO

Purpose: Iris biological features such as surface features and profile characteristics reflected the development of iris stroma and microvessels. Iris vessels and microcirculation are still lack of effective detection methods, and we can directly observe only the iris surface biological characteristics. This cross-sectional study evaluated the association between iris surface biological features and corneal biomechanics in young adults with myopia. Methods: We recruited 152 patients with myopia aged ≥18 years, from the Eye Hospital of Wenzhou Medical University, who had complete Corneal Visualization Scheimpflug Technology (Corvis ST) data and graded iris surface features. Iris surface features included crypts, furrows, and color measured from digital slit lamp images. The biomechanical properties of the cornea were assessed using Corvis ST. Only 1 eye of each participant was randomly selected for analysis. Associations between the iris surface features and corneal biomechanics were analyzed using linear regression models. The grade of iris crypts, furrows, and color and corneal biomechanical parameters measured with Corvis ST was the main outcome measures. Results: The iris crypts were significantly associated with deflection amplitude at the first applanation (A1 DLA, ß = 0.001, P = 0.013), A1 delta arc length (A1 dArcL) (ß = -0.001, P = 0.01), maximum delta arc length (dArcLM) (ß = -0.004, P = 0.03), and stiffness at the first applanation (SP-A1) (ß = -2.092, P = 0.016). The iris furrows were only associated with integrated radius (ß = -0.212, P = 0.025). Iris color was found not related with corneal biomechanical parameters measured via Corvis ST. Conclusions: Iris surface features were associated with corneal biomechanical properties in myopic eyes; more iris crypts were associated with lower corneal stiffness while more extensive furrows were related with higher corneal stiffness. Iris crypts and furrows may provide useful information on corneal biomechanical properties in myopic eyes.

8.
J Virol Methods ; 300: 114362, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34801595

RESUMO

A recombinase polymerase amplification (RPA) assay was established for the rapid detection of Decapod iridescent virus 1 using primers targeted to the virus's ATPase gene (ORF114R). Optimization experiments showed that the optimal amplification temperature of the RPA assay was 37 °C and that the reaction could be completed within only 15 min. The target band of 15 min. is bright enough. In order to shorten the operational reaction time, consequently, 15 min was the optimal amplification time for our new RPA assay for DIV1. Specificity tests showed that the RPA assay did not exhibit any cross-reactivity with other shrimp pathogens(TSV, MrNV, YHV-1, WSSV, EHP, AHPND, EHNV, RSIV, RGV and IHHNV). Sensitivity tests further showed that the detection limit of the new RPA assay was 200 copies/50 µL, indicating that this assay was more sensitive than a nested polymerase chain reaction (PCR) method. A total of 509 clinical samples were assayed using the RPA and the PCR assays; analysis showed that the RPA method could detect weak-positive samples more effectively than the PCR method. Collectively, these findings indicated that the RPA assay was fast, simple, specific, sensitive and has significant potentials for clinical and on-site testing.

9.
Cell Transplant ; 30: 9636897211053203, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34719306

RESUMO

Methionine sulfoxide reductase B1 (MsrB1) can catalyze both free and protein-bound R-methionine sulfoxides (R-MetO) to methionine (Met). It has been reported that MsrB1 plays an important role in the development of HCC and human bone osteosarcoma. However, little is known about the functions of MsrB1 in human colorectal cancer (CRC). Herein, we detected MsrB1 expression level in CRC tissue and cell lines, and investigated the effect of MsrB1 knockdown on CRC phenotypes and possible mechanisms involved in. The results showed that MsrB1 was highly expressed in both CRC tissues and cell lines, and that cell proliferation, migration and invasion were significantly inhibited, but apoptosis was increased after MsrB1 knockdown in colorectal cancer HCT116 and RKO cell lines, compared to control siRNA group. In addition, E-cadherin protein level was increased, vimentin and Snail protein were greatly decreased after knockdown of MsrB1 in cells. Furthermore, pGSK-3ß (Ser9) and ß-catenin protein levels were reduced, the promoter activity of TCF/LEF construction was inhibited after MsrB1 knockdown in cells, suggesting that GSK-3ß/ß-catenin signaling axis was involved in the tumorigenesis of CRC. In conclusion, the oncogenic role and related mechanisms of MsrB1 in CRC discovered in our work determined the potential role of MsrB1 as a biomarker and may provide a new target for clinical therapy of CRC.

10.
MAbs ; 13(1): 1981806, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34719342

RESUMO

Fragmentation is a well-characterized degradation pathway of therapeutic antibodies and is usually monitored by capillary electrophoresis-sodium dodecyl sulfate (CE-SDS). Although fragments due to cleavage in CH2 domains linked by intrachain disulfide bonds are common and can be detected by reduced reversed-phase - liquid chromatography mass spectrometry (RP-LCMS) and reduced CE-SDS methods, their separation in nonreduced CE-SDS (nrCE-SDS) has not been reported but speculated as comigrating with intact IgG. A shoulder peak in nrCE-SDS was observed in the stability samples of an IgG-like bispecific antibody and was determined to be mainly caused by fragments from clipping at the C-terminus of leucine (L)306 or L309 (EU numbering) in the CH2 domain of both heavy chains (HCs) and, to a lesser degree, at the C-terminus of L182 in the CH1 domain of the knob HC. Subunit LCMS analysis verified that the crystallizable fragment contained variants with one or multiple mass additions of ~18 Da due to clipping. Further investigation revealed that CH2 clippings at L306 and L309 were largely due to proteolytic activity, and cleavages were present at various levels in all in-house IgG1 and IgG4 molecules studied. Our study shows that CH2 domain cleavages, with complementary fragments still linked by intrachain disulfide, can be electrophoretically resolved as a front shoulder of the main peak in nrCE-SDS. Given the high occurrence of CH2 cleavages in antibodies, these findings will have broad applicability and could help manufacturers of therapeutic antibodies in process improvement, product characterization, investigations, formulation stability, and stability comparability studies.

11.
J Clin Transl Hepatol ; 9(5): 661-671, 2021 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-34722181

RESUMO

Background and Aims: Protein phosphatase 2A (PP2A) is associated with many cancers. This study aimed to clarify whether PPP2CA, which encodes the alpha isoform of the catalytic subunit of PP2A, plays a role in hepatocellular carcinoma (HCC) and to identify the potential underlying molecular pathways. Methods: Based on bioinformatics, public databases and our in-house RNA-Seq database, we analyzed the clinical value and molecular mechanism of PPP2CA in HCC. Results: Data were analyzed from 2,545 patients with HCC and 1,993 controls without HCC indexed in The Cancer Genome Atlas database, the Gene Expression Omnibus database and our in-house RNA-Seq database. PPP2CA expression was significantly higher in HCC tissue than in non-cancerous tissues (standardized mean difference: 0.69, 95% confidence interval [CI]: 0.50-0.89). PPP2CA expression was able to differentiate HCC from non-HCC, with an area under the summary receiver operator characteristic curve of 0.79 (95% CI: 0.75-0.83). Immunohistochemistry of tissue sections confirmed that PPP2CA protein was up-regulated in HCC tissues. High PPP2CA expression in HCC patients was associated with shorter overall, progression-free and disease-free survival. Potential molecular pathways through which PPP2CA may be involved in HCC were determined using miRWalk 2.0 as well as analysis of Gene Ontology categories, Kyoto Encyclopedia of Genes and Genomes pathways, and protein-protein interaction networks. Conclusions: PPP2CA is up-regulated in HCC and higher expression correlates with worse prognosis. PPP2CA shows potential as a diagnostic marker for HCC. Future studies should examine whether PPP2CA contributes to HCC through the candidate microRNAs, pathways and hub genes identified in this study.

12.
Adv Mater ; 33(44): e2104764, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34723435

RESUMO

Achieving highly efficient hydrogen evolution reaction (HER) in alkaline media is a great challenge. Single-atom catalysts with high-loading amount have attracted great interest due to their remarkable catalytic properties. Herein, by using nickel foam as the substrate, the authors design and precisely synthesize atomic ruthenium (Ru)-loaded nickel hydroxide ultrathin nanoribbons (R-NiRu) with a high atomic Ru loading amount reaching ≈7.7 wt% via a one-step hydrothermal method. The presence of concentrated Cl- in the synthetic system is beneficial for constructing ultrathin nanoribbons, which, with abundant edge OH groups, make it easy to trap atomic Ru. Taking advantage of the synergy between atomic Ru and the nanoribbon morphology of nickel hydroxide, R-NiRu exhibit a low overpotential of 16 mV for HER at 10 mA cm-2 and a Tafel slope of 40 mV dec-1 in aqueous 1.0 m KOH solution, which are superior to those of commercial Pt/C (overpotential of 17 mV at 10 mA cm-2 , Tafel slope of 43 mV dec-1 ). Density functional theory (DFT) calculation results demonstrate that atomically dispersed Ru can significantly reduce the HER energy barrier. Moreover, R-NiRu maintains exceptional stability after 5000 cyclic voltammetry cycles. This efficient and facile synthetic strategy provides a new avenue for designing efficient catalysts.

13.
Front Psychol ; 12: 742293, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777132

RESUMO

Grounded in the stress-coping model, our study examined family functioning and the classroom environment as protective factors in the relationship between daily stress and behavioral problems in Chinese children. The participants were 1,450 children (51.7% male, M age = 10.91 years, SD = 0.96) in the fourth, fifth, and sixth grades at five schools. The children completed the questionnaires measuring daily stress, family functioning, and the classroom environment. Additionally, their parents rated their behavioral problems. The latent moderated structural (LMS) equation approach was used to test moderator effects. After controlling for sex and grade, our results indicate that daily stress positively predicted the children's behavioral problems. Both family functioning and the classroom environment moderated the relationship between daily stress and behavioral problems. Further assessment of latent interaction effects indicate that buffering effects on behavioral problems were most prominent in conditions involving low stress. In sum, families and schools should not ignore children's minor stressors, as interventions involving family functioning and favorable classroom environments may help to reduce behavioral problems in children who report low levels of daily stress.

14.
Front Oncol ; 11: 768879, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34796115

RESUMO

Dihydroartemisinin (DHA), a well-known antimalarial drug, has been widely investigated for its antitumor effects in multiple malignancies. However, its effects and regulatory mechanisms in colorectal cancer (CRC) are still unproved. In this study, in vitro experiments including CCK8, EdU, Transwell, and flow cytometry analyses and an in vivo tumorigenesis model were conducted to assess the effects of DHA on the bio-behaviors of CRC cells. Additionally, RNA-seq combined with gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses was used to obtain the targets of DHA, and these were verified by molecular docking, qRT-PCR, and Western blotting. As a result, we found that DHA significantly suppressed the proliferation, DNA synthesis, and invasive capabilities and induced cell apoptosis and cell cycle arrest in HCT116, DLD1, and RKO cells in vitro and in vivo. Further analyses indicated that the targets of DHA were predominantly enriched in cell cycle-associated pathways, including CDK1, CCNB1, and PLK1; and DHA could bind with the CDK1/CCNB1 complex and inhibit the activation of CDK1/CCNB1/PLK1 signaling. Moreover, cucurbitacin E, a specific inhibitor of the CDK1/CCNB1 axis, enhanced the inhibitory effects of DHA on DNA synthesis and colony formation in HCT116 and DLD1 cells. In short, DHA could suppress the tumorigenesis and cycle progression of CRC cells by targeting CDK1/CCNB1/PLK1 signaling.

15.
Anim Sci J ; 92(1): e13658, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34788894

RESUMO

This study investigated the effects of diet supplementation with alkaline protease (AKP) on the production performance, egg quality, and cecal microbiota of laying hens. A total of 720 Hy-Line Brown laying hens (60 weeks old) were divided into four groups with six replicates of 30 birds each. No AKP was added to the control diet, and the hens in the other three groups (Groups 1, 2, and 3) were fed the basal diet supplemented with AKP preparations at 3, 6, and 9 u/g of diet, respectively. Results showed that AKP supplementation significantly decreased the feed/egg ratio (p < 0.05). Compared with that of the control group, the eggshell strength of Group 1 was significantly increased (p < 0.05), and the egg yolk weight of Groups 1 and 3 was significantly increased (p < 0.05). Distinctive difference in cecal microbiota was observed between AKP and control groups, and the average values of microbial diversity was lower in the AKP group than in the control group. The relative abundance of Bacteroidetes and Firmicutes at the phylum level, Rikenellaceae, Lachnospiraceae, Lactobacillaceae, Erysipelotrichaceae, and Christensenellaceae at the family level, and Rikenellaceae_RC9_gut_Group, Lactobacillus, Romboutsia, Lachnoclostridium, and Blautia at the genus level in the AKP group changed significantly compared with that in the control group (p<0.05).

16.
Sensors (Basel) ; 21(21)2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34770520

RESUMO

This paper proposes a parameter identification method for the multiparameter identification study of the linear-arch composite beam piezoelectric energy harvester. According to the voltage response characteristics of the system under short-circuit conditions, the mechanical equation is solved by transient excitation, combined with the backbone curve theory and logarithmic attenuation method, to obtain the system's linear damping, linear stiffness, and nonlinear stiffness. According to the voltage response characteristics of the system under open-circuit conditions, combined with the electrical equations, the system electromechanical coupling coefficient and equivalent capacitance coefficient are obtained; numerical simulation results show that the identification parameters have good accuracy. Finally, an experimental platform was built for verification, and the results show that the method has high accuracy and practicability.

17.
Food Chem ; : 131567, 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34802803

RESUMO

Artemisia sphaerocephala Krasch. polysaccharide (ASKP) was found to be crosslinked with ferric ions to form hydrogels in the previous study. In this work, it was demonstrated that ASKP-Fe3+ hydrogel complexed with pullulan or gelatin contributed to a significantly enhanced gel strength at 1.5% ASKP, 60 mM Fe2+, pH 4.0, and the mixing ratio of 9: 1. The complexed hydrogels presented a dense semi-interpenetrating network along with the delay of gelation time and the increase of water retention. ASKP based complexes exhibited good compatibility, probably because pullulan and gelatin could be entangled with ASKP chain under hydrogen bonding and electrostatic interaction, respectively. The interaction between ASKP and pullulan or gelatin contributed to the formation of complexed hydrogels with dense network and significantly enhanced gel strength. It is inferred that ASKP would have great potential to be a new gelling material as well as for the ferric ions delivery.

18.
Clin Interv Aging ; 16: 1801-1812, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675495

RESUMO

Purpose: To determine whether combined performance-based models could exert better predictive values toward discriminating community-dwelling elderly with high risk of any-falls or recurrent-falls. Participants and Methods: This prospective cohort study included a total of 875 elderly participants (mean age: 67.10±5.94 years) with 513 females and 362 males, recruited from Hangu suburb area of Tianjin, China. All participants completed comprehensive assessments. Methods: We documented information about sociodemographic information, behavioral characteristics and medical conditions. Three functional tests-timed up and go test (TUGT), walking speed (WS), and grip strength (GS) were used to create combined models. New onsets of any-falls and recurrent-falls were ascertained at one-year follow-up appointment. Results: In total 200 individuals experienced falls over a one-year period, in which 66 individuals belonged to the recurrent-falls group (33%). According to the receiver operating characteristic curve (ROC), the cutoff points of TUGT, WS, and GS toward recurrent-falls were 10.31 s, 0.9467 m/s and 0.3742 kg/kg respectively. We evaluated good performance as "+" while poor performance as "-". After multivariate adjustment, we found "TUGT >10.31 s" showed a strong correlation with both any-falls (adjusted odds ratio (OR)=2.025; 95% confidence interval (CI)=1.425-2.877) and recurrent-falls (adjusted OR=2.150; 95%CI=1.169-3.954). Among combined functional models, "TUGT >10.31 s, GS <0.3742 kg/kg, WS >0.9467 m/s" showed strongest correlation with both any-falls (adjusted OR=5.499; 95%CI=2.982-10.140) and recurrent-falls (adjusted OR=8.260; 95%CI=3.880-17.585). And this combined functional model significantly increased discriminating abilities on screening recurrent-fallers than a single test (C-statistics=0.815, 95%CI=0.782-0.884, P<0.001), while not better than a single test in predicting any-fallers (P=0.083). Conclusion: Elderly people with poor TUGT performance, weaker GS but quicker WS need to be given high priority toward fall prevention strategies for higher risks and frequencies. Meanwhile, the combined "TUGT-, GS-, WS+" model presents increased discriminating ability and could be used as a conventional tool to discriminate recurrent-fallers in clinical practice.


Assuntos
Vida Independente , Velocidade de Caminhada , Idoso , China , Feminino , Força da Mão , Humanos , Masculino , Equilíbrio Postural , Estudos Prospectivos , Estudos de Tempo e Movimento
19.
BMC Med Imaging ; 21(1): 152, 2021 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-34666701

RESUMO

BACKGROUND: Screening of breast cancer in asymptomatic women is important to evaluate for early diagnosis. In China ultrasound is a more frequently used method than mammography for the detection of breast cancer. The objectives of the study were to provide evidence and assessment of parenchymal patterns of ultrasonography for breast cancer detection among Chinese women. METHODS: Breast ultrasound examinations including the parenchymatous pattern of cytopathological confirmed breast cancer (n = 541) and age-matched cytopathological not confirmed breast cancer (n = 849) women were retrospectively reviewed by seven sonographer physicians. According to compositions of ducts, the thickness of the breast, diameter of ducts, fat lobules, and fibro glandular tissues, the breast parenchymatous pattern was categorized into heterogeneous (high percentage of fatty tissues), ductal (the inner diameters of ducts > 50% of the thick mass of the breast), mixed (the inner diameters of ducts was 50% of the thick mass of the breast), and fibrous categories (a dense classification of the breast). RESULTS: Heterogeneous (p < 0.0001, OR = 3.972) and fibrous categories (p < 0.0001, OR = 2.702) were higher among women who have cytopathological confirmed breast cancer than those who have not cytopathological confirmed breast cancer. The heterogeneous category was high-risk ultrasonographic examination category followed by the fibrous category. Agreements between sonographer physicians for categories of ultrasonic examinations were fair to good (Cohen's k = 0.591). CONCLUSIONS: Breast cancer risk in Chinese asymptomatic women differ according to the ultrasonographic breast parenchymal pattern. LEVEL OF EVIDENCE: III. Technical efficacy stage: 2.

20.
J Reprod Dev ; 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34657902

RESUMO

Hormonal products have been developed for fixed-time artificial insemination (FTAI) to improve the efficiency of swine production. Here, we evaluated the effect of an FTAI protocol initiated during different phases of the estrous cycle on follicle development and ovulation in gilts. A total of 36 gilts were equally divided into three groups designated as the luteal (L), follicular (F), and post-ovulation (O) groups and fed with 20 mg of altrenogest for 18 days, followed by intramuscular injection of 1000 IU PMSG at 42 h after withdrawal of altrenogest, and 100 µg of GnRH after an 80-h interval. The L group had the highest number of follicles 4-6 mm in diameter, as well as corpora hemorrhagica. The mRNA expression of caspase-9 in the L group were significantly lower than those in the O and F groups (P < 0.05), while CYP11A1 and VEGF mRNA expression levels were significantly higher (P < 0.05). Moreover, FSHR mRNA levels were significantly higher in the O group than in the L, F, and control groups (P < 0.05). LHCGR and CYP19A1 mRNA levels were the highest in the F group (P < 0.05). Thus, the changes in the expression of genes associated with follicular development, maturation, and ovulation identified in this study indicated that initiation of the FTAI protocol during the luteal phase induced a better environment for follicle development and ovulation in gilts.

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