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1.
J Agric Food Chem ; 72(14): 8257-8268, 2024 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-38530904

RESUMO

Rapid and accurate detection of the zoonotic nematode Anisakis is poised to control its epidemic. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-associated assay shows great potential in the detection of pathogenic microorganisms. The one-tube method integrated the CRISPR system with the recombinase polymerase amplification (RPA) system to avoid the risk of aerosol pollution; however, it suffers from low sensitivity due to the incompatibility of the two systems and additional manual operations. Therefore, in the present study, the agarose hydrogel boosted one-tube RPA-CRISPR/Cas12a assay was constructed by adding the CRISPR system to the agarose hydrogel, which avoided the initially low amplification efficiency of RPA caused by the cleavage of Cas12a and achieved reaction continuity. The sensitivity was 10-fold higher than that of the one-tube RPA-CRISPR/Cas12a system. This method was used for Anisakis detection within 80 min from the sample to result, achieving point-of-care testing (POCT) through a smartphone and a portable device. This study provided a novel toolbox for POCT with significant application value in preventing Anisakis infection.


Assuntos
Anisakis , Animais , Anisakis/genética , Recombinases , Sistemas CRISPR-Cas , Sefarose , Sistemas Automatizados de Assistência Junto ao Leito , Hidrogéis , Nucleotidiltransferases , Técnicas de Amplificação de Ácido Nucleico
2.
Artigo em Inglês | MEDLINE | ID: mdl-38551424

RESUMO

Objective: To analyze the construction and effect of standardized procedure of early activity after cardiac surgery in elderly patients based on the critical illness scoring system. Methods: A total of 65 elderly patients who underwent cardiac surgery in our hospital from January 2020 to January 2022 were selected as the research objects and divided into the control group (n = 32) and the observation group (n = 33) according to the admission time. The standardized procedure for the early activity after cardiac surgery was implemented based on the critical illness scoring system. The inter-group comparison was conducted in terms of the recovery time, complications, cardiac function, and quality of life pre- and post-nursing. Results: The observation group recovered faster than the control group following nursing care. The incidence rate of complications was 6.06% in the observation group, noticeably lower than 25.00% in the control group. The observation group exhibited remarkably higher left ventricular ejection fraction (LVEF), noninvasive cardiac output (NICO), and stroke volume (SV) but lower left ventricular end-diastolic dimension (LVED) post-nursing than the control group, which indicated that the cardiac function index was superior in the observation group. Following the nursing, the observation group attained higher scores in each item of the World Health Organization Quality of Living Scale (WHOQOL-BREF) than the control group. Conclusion: The critical illness scoring system is of significant value in constructing a standardized process of early postoperative cardiac surgery in the elderly, which can effectively promote postoperative recovery, reduce the occurrence of complications, protect cardiac function, and improve the quality of life of patients affected.

3.
BMC Geriatr ; 24(1): 147, 2024 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-38350861

RESUMO

BACKGROUND: The relationship of testosterone and estradiol concentrations with cognitive function among community-dwelling older men was inconclusive. To examine the association of serum testosterone and estradiol concentrations with cognitive function in older men with or without vascular risk factors (VRFs). METHODS: This cross-sectional study consisted of 224 community-dwelling men aged 65-90 years in the Songjiang District of Shanghai, China. Serum testosterone and estradiol were measured by electrochemiluminescence immunoassay. The following five factors were defined as VRFs in this study: obesity, history of hypertension, diabetes, stroke, and coronary heart disease. Multivariable linear regression was used to examine the association of testosterone and estradiol with the Mini-Mental State Examination (MMSE) in participants with or without VRF. Restricted cubic spline (RCS) regression was performed to account for the nonlinearity of these associations. RESULTS: An inverted "U" shaped non-linear relationship was found between testosterone concentration and MMSE score in men with one VRF (P overall =.003, non-linear P =.002). Estradiol showed an inverted "U" shaped non-linear relationship with MMSE score independent of VRFs (men without VRF, P overall =.049, non-linear P =.015; men with one VRF, overall P =.007, non-linear P =.003; men with two or more VRFs, overall P =.009, non-linear P =.005). CONCLUSION: In older men, an optimal level of sex steroid concentration may be beneficial to cognitive function and the VRFs should be considered when interpreting the relationship between sex steroid and cognitive function.


Assuntos
Cognição , Estradiol , Hormônios Esteroides Gonadais , Idoso , Humanos , Masculino , China/epidemiologia , Estudos Transversais , Estradiol/sangue , Hormônios Esteroides Gonadais/sangue , Vida Independente , Fatores de Risco , Testosterona
4.
Int J Biol Macromol ; 253(Pt 8): 127330, 2023 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-37832623

RESUMO

Exopolysaccharides (EPS) are natural, nontoxic, biocompatible and biodegradable macromolecules produced by microorganisms, including the Lactic acid bacteria, to enhance protection against environmental stress conditions. The current study focused on the encapsulation and functional efficiency of EPS produced by probiotic strains isolated from human milk. Among 27 isolates, the potential high EPS-producing strain Limosilactobacillus reuteri KCTC 14626BP was selected based on biofilm production. The structural Characterization of EPS was performed based on FTIR, NMR and functional properties were determined; further, the encapsulation efficiency of EPS was determined with caffeic acid. The results indicate that L. reuteri produced EPS major component consisting of glucose, galactose and arabinose with the ratio of (0.78:0.16: 0.05). The antioxidant efficiency of EPS-LR was determined on DPPH (60.3 %) and ABTS (48.9 %); EPS showed enhanced functional activities. The absence of toxicity was confirmed based on Caenorhabditis elegans. The EPS-loaded Caffeic acid (CA) EPS-LR indicated spherical capsules with rough surfaces, with sizes ranging from 1.39 to 6.75 µm. These findings indicate that EPS-LR can be applied as a bioactive compound and encapsulating material in food, cosmetics, and pharmaceutical industries.


Assuntos
Limosilactobacillus reuteri , Polissacarídeos Bacterianos , Feminino , Humanos , Polissacarídeos Bacterianos/química , Leite Humano , Ácidos Cafeicos
5.
Front Bioeng Biotechnol ; 11: 1181448, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37214289

RESUMO

The lysosome is an important target for realizing antitumor therapy. Lysosomal cell death exerts significant therapeutic effects on apoptosis and drug-resistance. The development of lysosome-targeting nanoparticles to obtain efficient cancer treatment is challenging. In this article, nanoparticles composed of DSPE@M-SiPc and possessing bright two-photon fluorescence, lysosome targeting ability, and photodynamic therapy multifunctionalities are prepared by encapsulating morpholinyl-substituted silicon phthalocyanine (M-SiPc) with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(poly(ethylene glycol))-2000] (DSPE). Two photon fluorescence bioimaging showed that M-SiPc and DSPE@M-SiPc mainly locate in lysosomes after cellular internalization. Upon irradiation, DSPE@M-SiPc effectively generates reactive oxygen species and damages the function of lysosome, subsequently leading to lysosomal cell death. DSPE@M-SiPc is a promising photosensitizer for cancer treatment.

6.
Front Immunol ; 14: 1101335, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36733488

RESUMO

Cyclic GMP-AMP Synthase (cGAS) is a pivotal adaptor of the signaling pathways involving the pattern recognition receptors and plays an important role in apoptosis and immune regulation. The cGAS function in mammals has been investigated extensively; however, the function of duck cGAS (du-cGAS) in response to viral infections is still unclear. This study aimed to clone the mallard (Anas platyrhynchos) cGAS homolog to investigate the function of duck cGAS (du-cGAS) in host antiviral innate immunity. The results showed that the open reading frame (ORF) region of the du-cGAS gene was 1296 bp, encoding 432 amino acids (aa) and exhibiting similar functional domains with its chicken counterpart. Knockdown of the endogenous du-cGAS by specific sgRNA strongly increased the replication of DNA viruses, including duck adenovirus B2 (DAdV B2) and duck short beak and dwarfism syndrome virus (SBDSV). However, the knockout did not impair the replication of novel duck reovirus (NDRV), an RNA virus. Furthermore, the mRNA expressions of type I interferon (IFNs) and vital interferon-stimulated genes (ISGs) were remarkably reduced in the du-cGAS knockout DEF cell line. Inversely, du-cGAS overexpression greatly activated the transcription of IFN-α, IFN-ß, and vital ISGs, and impaired the replication of DAdV B2, SBDSV, and NDRV in the DEF cell line. Importantly, we found that a deletion of 68 aa in the N terminus didn't impair the antiviral function of du-cGAS. Overexpressing NTase Core, C-Domain (Mab21), or Zinc-Ribbon domain independently had no antiviral effects. Generally, these results reveal that du-cGAS is a vital component of the innate immune system of ducks, with a universal antiviral activity, and provides a useful strategy for the control of waterfowl viral diseases.


Assuntos
Orthoreovirus , Vírus de RNA , Viroses , Vírus , Animais , Interferons/metabolismo , Antivirais , RNA , Vírus/genética , Vírus de RNA/genética , Replicação Viral , DNA , Nucleotidiltransferases/genética , Nucleotidiltransferases/metabolismo , Mamíferos/metabolismo
7.
Gene ; 858: 147195, 2023 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-36641079

RESUMO

The gamma amino butyric acid (GABA) is a chemical messenger and is essential for the health of the brain and muscles. Some lactic acid bacteria (LAB) have the potential to function as psychobiotic cultures because they can produce significant amounts of neuroactive compounds like GABA. Psychobiotics are known to alter bidirectional communication between the gastrointestinal tract and the central nervous system. In the present study, the Limosilactobacillus reuteri (L. reuteri) strain, isolated from human breast milk, was used to detect the GABA-producing glutamic acid decarboxylase (gad) gene and GABA production. PCR, HPLC and UHPLCQ-TOF-MS2 approaches were applied to identify the gad gene, GABA content, and bioactive compounds produced by the bacterial strain, respectively. Additionally, the whole genome was sequenced to better understand the strain's psychobiotic and technological genomic properties. The gadB and gadC genes were confirmed in plasmid 1 of the whole genome. The complete genome sequence of L. reuteri comprises the genome length of 2,087,202 bp with 51.6 percent of G + C content. The results indicate that L. reuteri can be used as a starter culture for the production of GABA-enriched functional foods as well as psychobiotics for health benefits.


Assuntos
Limosilactobacillus reuteri , Humanos , Limosilactobacillus reuteri/genética , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem , Ácido gama-Aminobutírico , Plasmídeos
8.
Front Microbiol ; 13: 1026129, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36532447

RESUMO

Anisakidosis is a food-borne parasitic disease (FBPD) caused by the third-stage larvae of the family Anisakidae. Therefore, it is important to develop a simple, rapid and equipment-free detection method for anisakids in fish samples or seafood since current methods are time-consuming and require complex instruments. In this study, a recombinase polymerase amplification (RPA)-based method was established for the first time to detect anisakids by targeting the internal transcribed spacer (ITS) regions. The detection results were visualized by including SYBR Green I (SG) in the method. The sensitivity of RPA-SG assay was 102 copies per reaction of recombinant plasmid (within 20 min at 37°C), similar to quantitative real-time PCR (qPCR). The assay had high specificity for detecting anisakids against other related parasites and host fish. In addition, the assay was further used to detect fresh marine fish contaminated with anisakids and it showed high precision. These results indicate that the novel RPA-SG assay suitable for visual detection of anisakids in the field and food safety control.

9.
Insects ; 13(7)2022 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-35886801

RESUMO

The insecticide emamectin benzoate (EB) was formulated with nanoparticles composed of DSPE-PEG2000-NH2 by the co-solvent method to determine its adverse impacts on the environment and to reinforce its dispersion, adhesion, and biocompatibility. A good encapsulation efficiency (70.5 ± 1.5%) of EB loaded in DSPE-PEG2000-NH2 polymeric liposomes was confirmed. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and contact angle meter measurements revealed that the DSPE-EB nanoparticles had a regular distribution, spherical shape, and good leaf wettability. The contact angle on corn leaves was 47.26°, and the maximum retention was higher than that of the reference product. DSPE-EB nanoparticles had strong adhesion on maize foliage and a good, sustained release property. The efficacy trial showed that the DSPE-EB nanoparticles had a strong control effect on S. frugiperda larvae, with the LC50 of 0.046 mg/L against the third-instar S. furgiperda larve after 48 h treatment. All these results indicate that DSPE-EB nanoparticles can serve as an insecticide carrier with lower environmental impact, sustained release property, and effective control of pests.

10.
PLoS One ; 17(6): e0269403, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35657911

RESUMO

In the present study, white (Baegilmi), brown (hyunmi) and black (chalheugmi) Korean local rice varieties ethanol extracts were analyzed for in-vitro antioxidant assays (ABTS, FRAP and DPPH), cellular antioxidant activities (CAAs) and phenolic phytochemicals content. The highest antioxidant assays, phenolic, flavonoid and anthocyanins content were identified among the free fractions of black rice. Phenolic phytochemicals were detected and quantified using the ultra-high-performance liquid tandem chromatography quadrupole flight mass spectrometry (UHPLC-Q-TOF-MS2). Which indicated the richness of several phytochemicals like ascorbic acid, vanillic acid, p-coumaric acid, catechin, epigallocatechin and quercetin in black rice than in other rice samples. The cellular antioxidant activities (CAA) of black rice were found equivalent to that of ascorbic acid, the standard employed in the assay. The CAAs of free fractions were as follows: white rice < brown rice < black rice. These findings are significant for enhancing human health through increased consumption of black and brown rice in the development of functional food products.


Assuntos
Adenocarcinoma , Neoplasias Colorretais , Oryza , Antocianinas/análise , Antocianinas/farmacologia , Antioxidantes/química , Humanos , Estresse Oxidativo , Fenóis/química , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química
11.
BMC Geriatr ; 22(1): 414, 2022 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-35546663

RESUMO

BACKGROUND: Metabolic Syndrome (MetS) is a common health problem among older adults. Previous studies have revealed the relationship between sleep duration as well as global sleep status and MetS. OBJECTIVES: This study aims to examine the association between the specific sleep characteristic and MetS as well as MetS components among community-dwelling old adults. METHODS: This cross-sectional study included 1499 community residents aged ≥ 60 years. Sleep characteristics were assessed using the Pittsburgh Sleep Quality Index (PSQI) and bed/rise time of the residents. Logistic regression analysis and multiple linear regression analysis were used to examine the associations between sleep characteristics and MetS as well as MetS components. A generalized additive model was built to assess the smooth relationship between triglyceride (TG) levels and sleep duration. RESULTS: Of the 1499 participants, 449 (30.0%) had MetS, and 443 (29.6%) had poor sleep quality. The rise time was found to be associated with MetS (> 6:00 vs. 5:00 ~ 6:00: adjusted OR (95%) = 1.77 (1.17-2.69), P = 0.007). For the MetS components, a U-shaped relationship was first revealed for sleep duration and TG levels (EDF = 1.85, P < 0.001). Furthermore, significant associations also included the associations of subjective sleep quality and daytime dysfunction with hypertension, the associations of sleep efficiency and rise time with hyperglycemia, the associations of rise time with TG levels, and the association of bedtime with waist circumference. CONCLUSIONS: The different sleep characteristics were associated with different MetS components.


Assuntos
Síndrome Metabólica , Idoso , Estudos Transversais , Humanos , Síndrome Metabólica/diagnóstico , Síndrome Metabólica/epidemiologia , Fatores de Risco , Sono , Circunferência da Cintura
12.
Front Microbiol ; 13: 865918, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35633663

RESUMO

In the natural environment, most microorganisms live in mixed-species biofilms, in which the metabolism and growth of organisms are different from that in single-species biofilms. Adhesive bacteria and their biofilms on the surface of food processing equipment are the sources of cross-contamination, leading to the risk for humans. Slightly acidic electrolyzed water (SAEW) has been proposed as a novel sanitizer in the food and agriculture industry. In this study, we investigated the changes in the physical properties of SAEW under different conditions and the disinfection abilities of SAEW against spore-forming and non-spore-forming pathogens. Furthermore, we examined the disinfection abilities of SAEW after 12 months of shelf life on a mixed-species biofilm of Listeria monocytogenes Scott A and Staphylococcus aureus. The results showed that SAEW at 30 and 50 ppm achieved all-kill of the spore-forming pathogen Bacillus cereus within 30 s. Changes in the ACC and pH of the produced SAEW were generally affected by the storage conditions. Both spore-forming and non-spore-forming pathogens were not detected under treatment with 50 ppm SAEW for 5 min under HDPE-closed conditions throughout the whole storage period. Moreover, 25 mg/L SAEW can inactivate L. monocytogenes Scott A and S. aureus biofilm cells in ~2.45 and 2.57 log CFU/mL in biofilms within 5-min treatment. However, the decline of the two bacteria in the mixed-species biofilm was 1.95 and 1.43 log CFU/mL, respectively. The changes in the cell membrane permeability of the mixed-species biofilm under treatment with SAEW were observed by using atomic force microscopy and confocal laser scanning microscopy. L. monocytogenes Scott A was more sensitive to SAEW in the mixed-species biofilm cells. These findings exhibited strong antibiofilm activities of SAEW in impairing biofilm cell membranes, decreasing cell density, and eliminating biofilm, which suggest that SAEW is an excellent antibacterial agent in the food processing industries.

13.
Microb Pathog ; 166: 105535, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35430267

RESUMO

Streptococcus mutans (S. mutans) can promote the establishment of high acidic biofilms and therefore have contribution to the development of dental caries. Alleviating the acidic environment and/or disrupting the structure of S. mutans biofilm are effective approaches against dental caries, rather than killing the microorganisms. The anti-biofilm effect of slightly acidic electrolyzed water (SAEW) is entirely based on the hypochlorous acid and ROS generation. In this study, sodium benzoate (NaB) acts as a pH adjuster and enhances SAEW's anti-biofilm activity. The results showed that the SAEW combined with NaB (SAEW + NaB) is highly effective in controlling biofilm. The adhesive strength of biofilm was significantly reduced by SAEW, and NaB was found to have a synergy effect with SAEW. Biofilm treated by SAEW + NaB was entirely removed by 60 s of ultrasonic wave, whereas the untreated biofilm can only be removed to a lesser extent. Atomic force microscope (AFM) analysis revealed that SAEW and NaB reduced the height of S. mutans biofilm. The metabolites derived from biofilm positively changed during the periodic 1-min treat, the production of lactic acid was hindered by the treatment. Altogether, these findings suggested a novel therapeutic intervention against S. mutans biofilm by targeting the cariogenic action.


Assuntos
Cárie Dentária , Streptococcus mutans , Biofilmes , Cárie Dentária/prevenção & controle , Humanos , Benzoato de Sódio/farmacologia , Água
14.
Transbound Emerg Dis ; 69(5): 2769-2778, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34921519

RESUMO

A new disease designated as Pale liver disease (PLD) has been circulating in Chinese Muscovy duck flocks since 2014, which is characterized by fatigue, diarrhoea, sudden death and acute hepatitis with pale and haemorrhagic liver. In this study, the etiological agents of PLD were isolated, causing a significant cytopathic effect (CPE) by cell rounding. Virus particles were observed by transmission electron microscopic (TEM) observation. The same disease was reproduced by experimental infection with the isolate BG61. The whole genomes of isolates were 43,842 nt in length with a GC content of 47.11%, similar to French Muscovy duck adenovirus strain GR with a GC content of 46.08%. The isolates shared 99.71-99.95% and 93.31-93.33% identity with Chinese Muscovy duck adenovirus isolates and GR strain, respectively. The DNA polymerase gene of all Muscovy duck adenovirus strains formed a separate genetic lineage with 99.55-100% amino acid sequence identity. All Chinese Muscovy duck adenovirus isolates contained two fibre genes. In contrast, only one fibre gene was found in GR, the only representative strain in species Duck aviadenovirus B. Anti-DAdV-2 serum antibodies had a weak neutralizing activity against Chinese Muscovy duck adenovirus isolates. The phylogenetic trees of the complete genome, hexon and fibre proteins revealed that all Muscovy duck adenovirus strains formed a major genetic lineage consisting of two clades. Thus, both GR and Chinese Muscovy duck adenovirus strains were proposed to be included in the same species of Duck aviadenovirus B belonging to the genus Aviadenovirus. The species Duck aviadenovirus B included two serotypes or genotypes, such as GR, which represents the strain of serotype 1 or genotype 1 (DAdV B1) and Chinese Muscovy duck adenovirus strains, which belong to serotype 2 or genotype 2 (DAdV B2).


Assuntos
Infecções por Adenoviridae , Aviadenovirus , Hepatite , Doenças das Aves Domésticas , Infecções por Adenoviridae/epidemiologia , Infecções por Adenoviridae/veterinária , Animais , China/epidemiologia , Patos , Filogenia , Doenças das Aves Domésticas/epidemiologia
15.
J Hazard Mater ; 424(Pt C): 127597, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34782200

RESUMO

Global spread of ampicillin (AMP) in the aquatic environment have attracted much attention recently. Marine anammox bacteria (MAB) have potentials in saline wastewater treatment due to their good salt tolerance. However, to date, the effect resulting from AMP on MAB is still unknown. Herein, the effect of AMP on MAB, involving microbial community evolution and genetic response, was investigated for the first time. A lab-scale reactor inoculated by MAB sludge was operated under saline condition (35 g/L) and AMP stress of different gradients. Within 200 cycles, nitrogen removal performance was monitored and sludge samples were withdrawn for high-throughput sequencing analyses and qPCR. The results confirmed that the nitrogen removal capacity of MAB declined with increasing AMP dosage, and almost collapsed at 300 mg/L AMP. The total nitrogen removal rate and specific anammox activity finally dropped to 0.17 kg N m-3 d-1 and 101.86 mg N g-1VSS d-1, respectively. Pseudoalteromonas (38.13%) dominated the reactor on Cycle 190, which formed a new symbiosis with MAB. And the emergence of oleophilic bacteria such as Colwellia (2.53%) was also observed. Moreover, antibiotic resistance genes were detected with increased abundance and diversity, indicating the AMP dosing significantly promoted microbial community evolution and genetic response.


Assuntos
Microbiota , Águas Residuárias , Ampicilina , Oxidação Anaeróbia da Amônia , Anaerobiose , Bactérias , Reatores Biológicos , Desnitrificação , Nitrogênio , Oxirredução , Água do Mar , Esgotos
16.
Ann Palliat Med ; 10(7): 8072-8081, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34353092

RESUMO

BACKGROUND: The Plan-Do-Check-Act cycle (PDCA) (also known as the Daiming cycle) is widely used in surgical management and can standardize nursing management and improve nursing quality. This meta-analysis evaluated the application of the PDCA cycle during nursing management following gynecological surgery. METHODS: PubMed, Web of Science, Embase, CNKI, Wanfang, and other databases were searched for studies on applying the PDCA cycle in nursing management following gynecological and obstetric surgery. Articles published between 2013 to 2020 in English and Chinese were included. The obtained data are subjected to meta-analysis using Stata16.0 analysis software. Reported outcomes included: satisfaction with care, nursing quality evaluation, and assessments using the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS). RESULTS: A total of 14 articles were included, with a total of 1,629 participants. Meta-analysis showed that satisfaction with gynecological surgery nursing using PDCA cycle management in the intervention groups was higher than in the control groups, OR =6.57 (95% CI, 4.01, 10.76), P<0.001. The perception of nursing quality was higher in the intervention groups than in the control groups, SMD =4.98 (95% CI, 3.32, 6.64, P<0.001. SAS scores of the intervention groups were lower than that of the control groups, SMD =-2.22 (95% CI, -2.80, -1.65), P<0.001. SDS scores of the intervention groups were lower than that of the control groups, SMD =-2.37 (95% CI, -3.15, -1.60), P<0.001. DISCUSSION: The application of PDCA cycle nursing management for gynecological surgery can significantly improve patients' satisfaction with nursing, including the quality of nursing. At the same time, it can effectively reduce the anxiety and depression of surgical patients, which benefits the surgery process. Given these benefits, it has a high likelihood of being incorporated into clinical practice.


Assuntos
Ansiedade , Cuidados de Enfermagem , Feminino , Procedimentos Cirúrgicos em Ginecologia , Humanos , Satisfação do Paciente
17.
Antioxidants (Basel) ; 10(7)2021 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-34356310

RESUMO

Oxidative stress has been postulated to play a role in several diseases, including cardiovascular diseases, diabetes, and stress-related disorders (anxiety/depression). Presently, natural plant-derived phytochemicals are an important tool in reducing metabolomic disorders or for avoiding the side effects of current medicinal therapies. Brown Rice (Oryza sativa L.) is an important part of Asian diets reported as a rich source of bioactive phytonutrients. In our present study, we have analyzed the effect of different lactic acid bacteria (LABs) fermentation on antioxidant properties and in the enhancement of bioactive constituents in Korean brown rice. Therefore, the antioxidant activities and phytochemical analysis were investigated for raw brown rice (BR) and different fermented brown rice (FBR). BR fermented with Limosilactobacillus reuteri, showed the highest antioxidant activities among all samples: DPPH (121.19 ± 1.0), ABTS (145.80 ± 0.99), and FRAP (171.89 ± 0.71) mg Trolox equiv./100 g, dry weight (DW). Total phenolic content (108.86 ± 0.63) mg GAE equiv./100 g, DW and total flavonoids content (86.79 ± 0.83) mg catechin equiv./100 g, DW was also observed highest in Limosilactobacillus reuteri FBR. Furthermore, phytochemical profiling using ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and cell antioxidant assay (CAA) revealed L. reuteri FBR as a strong antioxidant with an abundance of bioactive compounds such as gamma-aminobutyric acid, coumarin, cinnamic acid, butanoic acid, ascorbic acid, nicotinic acid, and stearic acid. This study expanded current knowledge on the impact of fermentation leading to the enhancement of antioxidant capacity with an abundance of health-related bioactive compounds in BR. The results obtained may provide useful information on functional food production using fermented brown rice.

18.
Microorganisms ; 9(6)2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34208588

RESUMO

The initiation and development of cariogenic (that is, caries-related) biofilms are the result of the disruption of homeostasis in the oral microenvironment. There is a daily accumulation of dental biofilm on the surface of teeth and its matrix of extracellular polymers supports the host in its defense against invading microbes, thus helping to achieve oral microbial homeostasis. However, the homeostasis can be broken down under certain circumstances such as during long-term exposure to a low pH environment which results in the dominance of acidogenic and acid-tolerating species in the dental biofilm and, thus, triggers the shift of harmless biofilm to an acidic one. This work aims to explore microbial diversity and the quorum sensing of dental biofilm and their important contributions to oral health and disease. The complex and multispecies ecosystems of the cariogenic biofilm pose significant challenges for the modulation of the oral microenvironment. Promising treatment strategies are those that target cariogenic niches with high specificity without disrupting the balance of the surrounding oral microbiota. Here, we summarized the recent advances in modulating cariogenic biofilm and/or controlling its pathogenic traits.

19.
Antioxidants (Basel) ; 10(4)2021 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-33921826

RESUMO

Free radical-induced oxidative stress is the root cause of many diseases, such as diabetes, stress and cardiovascular diseases. The objective of this research was to screen GABA levels, antioxidant activities and bioactive compounds in brown rice. In this study, we first fermented brown rice with different lactic acid bacteria (LABs), and the best LAB was selected based on the levels of GABA in the fermentate. Lactobacillus reuterii generated the highest levels of GABA after fermentation. To ascertain whether germination can improve the GABA levels of brown rice, we compared the levels of GABA in raw brown rice (Raw), germinated brown rice (Germ), fermented brown rice (Ferm) and fermented-germinated brown rice (G+F) to identify the best approach. Then, antioxidant activities were investigated for Raw BR, Germ BR, Ferm BR and G+F BR. Antioxidant activity was calculated using a 2,2-diphenyl-1-picryl hydrazile radical assay, 2,2-azino-bis-(3-ethylene benzothiozoline-6-sulfonic acid) radical assay and ferric-reducing antioxidant power. In Ferm BR, DPPH (114.40 ± 0.66), ABTS (130.52 ± 0.97) and FRAP (111.16 ± 1.83) mg Trolox equivalent 100 g, dry weight (DW), were observed as the highest among all samples. Total phenolic content (97.13 ± 0.59) and total flavonoids contents (79.62 ± 1.33) mg GAE/100 g and catechin equivalent/100 g, DW, were also found to be highest in fermented BR. Furthermore, an untargeted metabolomics approach using ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of bioactive compounds in fermented BR, such as GABA, tryptophan, coumaric acid, L-ascorbic acid, linoleic acid, ß-carotenol, eugenol, 6-gingerol, etc., as well as bioactive peptides which could contribute to the health-promoting properties of L. reuterii fermented brown rice.

20.
Poult Sci ; 100(2): 482-487, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33518100

RESUMO

H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was recently discovered and proposed to cause runting-stunting syndrome and urate deposition in geese. At present, however, there is a lack of epidemiological information pertaining to the dynamics and distribution of H146-like GCV. The development of novel molecular diagnostic approaches capable of rapidly and accurately detecting this virus would support the strengthening, the prevention, and control of H146-like GCV infection. In the present study, we therefore used a TaqMan probe and primers specific for the viral nonstructural (NS) gene to develop a highly sensitive and specific PCR assay capable of detecting this H146-like GCV. The assay reproducibly detected 5.07 × 102 copies of a recombinant DNA plasmid containing the NS gene, with a dynamic range of 8 orders of magnitude (102-109 copies). Importantly, no cross-reactivity was observed with common viruses that affected waterfowl, and when we used this assay to evaluate clinical samples, we found it to be more sensitive and faster than traditional PCR. In summary, herein, we developed a novel TaqMan-based real-time PCR approach that could reliably detect and diagnose H146-like GCV. This tool will allow for the real-time diagnosis of H146-like GCV infections, enabling researchers to better understand the epidemiology and clinical presentation of this disease.


Assuntos
Infecções por Caliciviridae/veterinária , Caliciviridae/isolamento & purificação , Gansos , Doenças das Aves Domésticas/virologia , Animais , Caliciviridae/genética , Infecções por Caliciviridae/virologia , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
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