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1.
Chemosphere ; 315: 137751, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36608876

RESUMO

Glyphosate, the most widely used herbicide worldwide, has been reported to cause hepatotoxicity. However, these systematic mechanisms remain poorly understood. Here, we investigated the effects of glyphosate-based herbicides (GBH) on liver toxicity in mice exposed to 0, 50, 250, and 500 mg/kg/day GBH for 30 d. Pathological and ultrastructural changes, serum biochemical indicators, oxidative stress state, and transcriptome and key protein alterations were performed to describe the hepatic responses to GBH. GBH induced hepatocytes structural alterations, vacuolation, and inflammatory, mitochondrial swelling and vacuolization; damaged liver function and aggravated oxidative stress; blocked the respiratory chain, promoted gluconeogenesis, fatty acid synthesis and elongation, and activated complement and coagulation cascades system (CCCS) in the liver. Moreover, SOD, H2O2, and MDA were negatively correlated with the CxI and CxIV genes, but positively correlated with the genes in glucolipid metabolism and CCCS pathways; however, the opposite results were observed for CAT, GSH-Px, and T-AOC. Overall, this study revealed the systematic mechanism underlying hepatotoxicity caused by GBH, providing new insights into understanding the hepatotoxicity of organophosphorus pesticide.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Herbicidas , Praguicidas , Animais , Camundongos , Herbicidas/toxicidade , Peróxido de Hidrogênio/farmacologia , Compostos Organofosforados/farmacologia , Praguicidas/farmacologia , Estresse Oxidativo , Inflamação/induzido quimicamente , Metabolismo Energético
2.
ACS Omega ; 8(3): 2897-2906, 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36713691

RESUMO

Background: Continuous cropping leads to the accumulation of autotoxic substances in faba beans, which limits their global production. Intercropping is widely used to alleviate these problems. Aim: This study aims to explore the important role of Fusarium oxysporum f. sp. fabae (FOF) and benzoic acid stress in enhancing the occurrence of faba bean Fusarium wilt and the potential mechanism of faba bean-wheat intercropping to control the occurrence of this disease. Methods: We analyzed the pathogenic mechanism of FOF and benzoic acid and the defense response of faba bean-wheat intercropping against the autotoxicity of benzoic acid under hydroponic conditions that included the pathogen alone and in combination with different concentrations of benzoic acid. Results: The dual stress of FOF and benzoic acid inhibited the activity of defensive enzymes, the synthesis of defensive substances, and the expression of defensive genes in faba bean roots and reduced the disease resistance of faba bean. This shows that benzoic acid plays an important role in helping FOF cause disease. Faba bean-wheat intercropping improves plant resistance by alleviating benzoic acid stress and reducing the incidence and disease index of Fusarium wilt. Conclusion: The dual stress of FOF and benzoic acid promotes the occurrence of faba bean Fusarium wilt by destroying the root defense system of faba bean. Faba bean-wheat intercropping can effectively alleviate the autotoxicity of benzoic acid and control the occurrence of Fusarium wilt by improving the physiological and biochemical resistance of faba beans and the expression of defense genes.

5.
Brain Pathol ; 33(1): e13113, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36634215

RESUMO

Spinal cord ischemia/reperfusion injury (SCII) is a severe complication driven by apoptosis and neuroinflammation. An increase in the expression of c-Fos, a member of the AP-1 family, is known as a neuronal activation marker in SCII. The AP-1 family is composed of Jun, Fos, and is associated with the regulation of cytokines expression and apoptosis. Fra-1 is a member of the Fos family, however, the contribution of Fra-1 to SCII is still unclear. In our study, Fra-1 was highly upregulated especially in neurons and microglia and promoted apoptosis by changing the expression of Bax/Bcl-2 after SCII. Furthermore, we found that Fra-1 directly regulated the transcription expression of S100A8. We demonstrated that knockdown of Fra-1 alleviated S100A8 mediated neuronal apoptosis and inflammatory factor release, thus improved motor function after SCII. Interestingly, we showed that administration of TAK-242, the TLR4 inhibitor, to the ischemia/reperfusion (I/R) injury induced rats suppressed the activation of the ERK and NF-κB pathways, and further reduced Fra-1 expression. In conclusion, we found that Fra-1-targeted S100A8 was expressed the upstream of Fra-1, and the Fra-1/S100A8 interaction formed a feedback loop in the signaling pathways activated by SCII.


Assuntos
Traumatismo por Reperfusão , Isquemia do Cordão Espinal , Ratos , Animais , Receptor 4 Toll-Like/metabolismo , Ratos Sprague-Dawley , Doenças Neuroinflamatórias , Fator de Transcrição AP-1/metabolismo , Medula Espinal/metabolismo , Isquemia do Cordão Espinal/metabolismo , Apoptose , Traumatismo por Reperfusão/metabolismo
6.
J Dent Sci ; 18(1): 27-33, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36643269

RESUMO

Background/purpose: The root fracture resistance of endodontically treated teeth is decreased significantly, and it is more likely to fracture. This study aimed to evaluate the effect of a novel root canal sealer based on bioactive glass (BG) on root fracture resistance and explore its mechanism. Materials and methods: The BG-based root canal sealer (BG Sealer) was prepared by mixing a kind of bioactive glass (10.8% P2O5, 54.2% SiO2, 35% CaO, mol.%, named PSC), zirconia (ZrO2) powder, sodium alginate (SA) and phosphate solution (PS). A pH meter was used to measure the pH of simulated body fluid (SBF) after immersion with BG Sealer at different time. After preparing the samples of BG sealer with a diameter of 4 mm and a height of 6 mm, the compressive strength was tested by a universal testing machine. The scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were used to detect and analyze the mineral status of root canal systems filled with BG Sealer. The push out test was used to measure the push out bond strength of BG Sealer. The fracture resistance of root canals filled with BG Sealer was detected by the compressive loading test. Bioceramic root canal sealer iRoot SP was set as the control group. Results: (1) Physicochemical properties: The pH value of SBF immersed with BG Sealer increased slightly up to 7.68, while the pH of SBF immersed with iRoot SP increased to 12.08. The compressive strength of the novel BG Sealer was 4.62 ± 1.70 MPa, which was lower than that of iRoot SP (P < 0.05). (2) Mineralization: The hydroxyapatite layers were observed on the surface of BG Sealer and iRoot SP after being immersed in SBF for 4 weeks. BG Sealer and iRoot SP were both able to penetrate into the dentin tubules, duplicate the morphology of root canals well, and form a layer of hydroxyapatite. (3) Adhesion to dentin: There was no significant difference between the push out bond strength of the novel BG Sealer and iRoot SP (P > 0.05). (4) Fracture resistance: After immersion in SBF for 4 weeks, the fracture resistance of roots filled with BG Sealer and iRoot SP was 454.16 ± 155.39 N and 445.50 ± 164.73 N, respectively, both of which were not statistically different from that of the roots unprepared and unfilled (394.07 ± 62.12 N) (P > 0.05), whereas higher than that of the roots prepared and unfilled (235.36 ± 83.80 N) (P < 0.05). Conclusion: The novel BG Sealer has good adhesion to the root dentin, can penetrate into the dentin tubules to generate minerals, and meanwhile can improve the fracture resistance of the roots after root canal treatment. It is expected to be a bioactive root canal sealer with good clinical application prospects.

7.
Dalton Trans ; 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36648310

RESUMO

Rational construction of nanosized anode nanomaterials is crucial to enhance the electrochemical performance of lithium-/sodium-ion batteries (LIBs/SIBs). Various anode nanoparticles are created mainly via templating surface confinement, or encapsulation within precursors (such as metal-organic frameworks). Herein, low-content SnO2 nanodots on N-doped reduced graphene oxide (SnO2@N-rGO) were prepared as anode nanomaterials for LIBs and SIBs, via a distinctive lattice confinement of a CoAlSn-layered double hydroxide (CoAlSn-LDH) precursor. The SnO2@N-rGO composite exhibits the advantagous features of low-content (17.9 wt%) and uniform SnO2 nanodots (3.0 ± 0.5 nm) resulting from the lattice confinement of the Co and Al species to the surrounded Sn within the same crystalline layer, and high-content conductive rGO. The SnO2@N-rGO composite delivers a highly reversible capacity of 1146.2 mA h g-1 after 100 cycles at 0.1 A g-1 for LIBs, and 387 mA h g-1 after 100 cycles at 0.1 A g-1 for SIBs, outperforming N-rGO. Furthermore, the dominant capacitive contribution and the rapid electronic and ionic transfer, as well as small volume variation, all give rise to the enhancement. Precursor-based lattice confinement could thus be an effective strategy for designing and preparing uniform nanodots as anode nanomaterials for electrochemical energy storage.

8.
Nurse Educ ; 48(1): E11-E16, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36137289

RESUMO

BACKGROUND: Peer feedback (PF) has attracted much attention in health profession education with growing evidence on its benefits. However, it lacks a proper framework to guide its operation, which raises concerns about its quality in nursing education. PURPOSE: To compare the effects of a 3-phase PF versus faculty feedback (FF) on students' reflective abilities and clinical competencies after simulated practice, and its impacts on peer tutors' feedback practices and empowerment level. METHODS: This study used a 3-arm pretest-posttest quasi-experimental methodology. RESULTS: Peer verbal feedback significantly increased students' reflective abilities and clinical competencies, while peer video feedback significantly increased clinical competencies. However, FF outcomes did not reach significance. Peer tutors' empowerment level did not significantly improve, but feedback practices were perceived as comparable with FF. CONCLUSIONS: Peer feedback can potentially improve students' reflective abilities and clinical competencies. Proper feedback training with a structured framework will likely enhance peer tutors' feedback practices.


Assuntos
Estudantes de Enfermagem , Humanos , Competência Clínica , Pesquisa em Educação de Enfermagem
9.
J Pharm Biomed Anal ; 224: 115191, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36512868

RESUMO

In this study, we investigated the effect of type 1 diabetes mellitus on the modulation of the activities of CYP450s in dynamics by a UHPLC-MS/MS method. The diabetic rat model was constructed by an intraperitoneal single injection of streptozotocin. Fasting blood glucose levels > 16.7 mmol/L were considered as diabetic. The rats were given a cocktail of four probe drugs (10 mg/kg phenacetin, 1 mg/kg tolbutamide, 10 mg/kg metoprolol, and 10 mg/kg midazolam) by oral administration for the pharmacokinetic study. Thereafter, the metabolic ratio (MR) of the metabolites to probe substrates were determined. The results indicated that two weeks after diabetes was induced, diabetes increased the MRs of acetaminophen/phenacetin (CYP1A2) and 4-hydroxyl tolbutamide/tolbutamide (CYP2C9); however, it decreased the MRs of α-hydroxy metoprolol/metoprolol (CYP2D6) and 1-hydroxy midazolam/midazolam (CYP3A4). Two months after diabetes was induced, diabetes increased the MRs of acetaminophen/phenacetin and 4-hydroxyl tolbutamide/tolbutamide. The MR of α-hydroxy metoprolol/metoprolol was decreased and the MR of 1-hydroxy midazolam/midazolam was increased but the difference was not significant. According to the results, CYP1A2 and CYP2C9 activities were enhanced in the diabetic rats. and CYP2D6 activity was inhibited in a short period of diabetes; however, the decrease in CYP2D6 activity was not significant in the long period. CYP3A4 activity was decreased in a short period of diabetes and increased in a long period of diabetes but was not significant in the two periods. This study suggests the activity change rule of the CYP450 enzyme system in diabetes mellitus, which can provide a reference for precise clinical medication.


Assuntos
Citocromo P-450 CYP1A2 , Diabetes Mellitus Experimental , Animais , Ratos , Acetaminofen , Cromatografia Líquida de Alta Pressão/métodos , Citocromo P-450 CYP2C9 , Citocromo P-450 CYP2D6 , Citocromo P-450 CYP3A , Sistema Enzimático do Citocromo P-450/metabolismo , Diabetes Mellitus Experimental/tratamento farmacológico , Metoprolol , Midazolam/metabolismo , Fenacetina , Espectrometria de Massas em Tandem/métodos , Tolbutamida
10.
J Hazard Mater ; 446: 130670, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36580787

RESUMO

The degradability improvement of poly(ethylene terephthalate) (PET), one of the most widely used but non-degradable disposable packaging material, is of great significance. However, the balance between degradability and mechanical properties remains a huge challenge. Herein, simple hydroxy acids, lactic acid (LA) and glycolic acid (GA) as easy hydrolysis sites were introduced into non-degradable PET via melt polycondensation. A series of high molecular weight poly(ethylene terephthalate-co-L­lactide) (PETL) and poly(ethylene terephthalate-co-glycolate) (PETG) copolyesters were synthesized with an excellent tensile strength greater than 50 MPa, much higher than that of most commercially available degradable polymers. The introduction of hydroxy acid endows PET with significantly improved composting and seawater degradation performance. Furtherly, the degradation rate of PETG with hydrophilic GA unit was faster than that of PETL, and the mineralization rate of PETG80 reaches 22.0%. The density of functional theory (DFT) calculation revealed that adding hydroxy acid to the PET molecular chain reduced the energy barrier of the hydrolysis reaction. The molecular polarity index (MPI) analysis furtherly confirmed that the higher affinity between the GA unit and water may be the primary reason for the faster degradation of PETG.

11.
J Hypertens ; 41(1): 150-158, 2023 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453658

RESUMO

BACKGROUND: The pathogenesis of preeclampsia (PE) is associated with inflammation and endothelial damage. Ulinastatin (UTI) mainly inhibits proteolytic activity and significantly reduces the release of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) from macrophages. It also ameliorates vascular endothelial damage in pathological conditions. Hence, we investigated the effects of UTI in a rat model of PE induced using N(gamma)-nitro-l-arginine methyl ester (L-NAME). METHODS: Although inducing PE in a rat model, 5000 U/kg of UTI were injected daily. Systolic blood pressure (SBP) and protein levels in the urine were measured. Renal function, and serum concentrations of TNF-α, IL-6, placental growth factor (PLGF), and von Willebrand factor (vWF) were evaluated. The number and weight of live fetuses as well as the weight of placentas were measured. Placentas were collected for western blot and pathological analysis. RESULTS: UTI slightly ameliorated proteinuria and the increases in SBP, blood urea nitrogen (BUN), and serum creatinine. Furthermore, UTI improved serum and placental protein expression levels of TNF-α, IL-6, vWF, and PLGF. Pathological analysis revealed that vascular density and blood flow perfusion was enhanced, vessel wall thickening and neutrophil infiltration were diminished, and the weight and number of live fetuses as well as the weight of the placentas were improved with UTI. CONCLUSION: Preventive use of UTI in the PE rat model induced by L-NAME partially alleviated hypertension, proteinuria, and impaired renal function; improved fetal growth restriction; diminished vascular endothelial injury; and ameliorated placental vasculogenesis abnormality and malperfusion by inhibiting the systemic and placental inflammatory response, suggesting that UTI is a potential drug for PE prevention or treatment.


Assuntos
Pré-Eclâmpsia , Feminino , Gravidez , Ratos , Animais , Humanos , Pré-Eclâmpsia/tratamento farmacológico , Placenta , Fator de Crescimento Placentário , Fator de von Willebrand , Fator de Necrose Tumoral alfa , Interleucina-6 , NG-Nitroarginina Metil Éster/farmacologia , Proteinúria/tratamento farmacológico
12.
J Affect Disord ; 323: 667-674, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36528133

RESUMO

BACKGROUND: Bullying is a growing concern worldwide. However, there is insufficient research on the prevalence and factors associated with different forms of bullying in a large sample of Chinese adolescents. This study aims to investigate the prevalence and associated factors of bullying victimization among Chinese high school students. METHODS: Data from the 2020-2021 cross-sectional survey of the DYH program were used. A total of 8203 high school students were randomly selected from all 17 cities in Shandong Province, China. Bullying victimization was measured using two separate questions and identified into three types: school bullying victims, cyberbullying victims, and combined bullying victims. The multinomial logit model was used to explore the factors related to bullying victimization. RESULTS: The prevalence of bullying victimization was 11.59 %, with 4.04 % of school bullying, 3.37 % of cyberbullying, and 4.18 % of combined bullying. Smoking was negatively associated with bullying victimizations. Male, physical exercise, peer relationships, and obsessive-compulsive symptoms were significantly associated with school bullying victims and combined victims. Watching TV was an influencing factor for school bullying victims and cyberbullying victims. Single-child households, family economic status, drinking alcohol, parent-child relationships, and paranoia were correlated with different types of bullying victimization. LIMITATIONS: The cross-sectional design limited the examination of causal inferences. CONCLUSION: We should pay more attention to cyberbullying victimization, which has become as common as school bullying victimization among high school students. The associated factors explored in this study may contribute to understanding bullying victimizations and designing bullying intervention strategies among high school students in China.


Assuntos
Bullying , Vítimas de Crime , Adolescente , Humanos , Masculino , China/epidemiologia , Estudos Transversais , Prevalência , Estudantes , Feminino
13.
Int J Cardiol ; 372: 113-119, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36513285

RESUMO

BACKGROUND: Intensive blood pressure (BP) lowering in patients with hypertension has been associated with a lowered risk of atrial fibrillation (AF). It is still uncertain what is the optimal BP levels to prevent AF in the general elderly population. In the present prospective study, we investigated the association between incident AF and BP in an elderly Chinese population. METHODS AND FINDINGS: Elderly (≥65 years) residents were recruited from 6 communities in Shanghai. 9019 participants who did not have AF at baseline and had at least one ECG recording during follow-up were included in the present analysis. During a median of 3.5 years follow-up, the overall incidence rate of AF was 5.6 per 1000 person-years (n = 178). Systolic BP was associated with increased AF risk (age- and sex-adjusted hazard ratio [HR] per 20-mmHg increase for systolic BP 1.21, 95% CI 1.04-1.39, P = 0.01), but risk estimate was attenuated after adjustment for common AF risk factors. In categorical analyses, statistical significance was achieved for HR relative to optimal BP only in stage 2 or 3 systolic and diastolic hypertension (multivariate-adjusted HR 1.76, 95% CI 1.00-3.08, P = 0.05). The association between AF incidence and BP status tended to be stronger in the absence than presence of a history of cardiovascular disease at baseline (P for interaction = 0.06). CONCLUSION: In this Chinese population of 65 years and older, linear increases in systolic and diastolic BP were not independently associated with increased risk of AF, and only exposure to stage 2 or 3 hypertension carries a higher risk of AF.


Assuntos
Fibrilação Atrial , Hipertensão , Humanos , Idoso , Pressão Sanguínea/fisiologia , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/epidemiologia , Fibrilação Atrial/complicações , Estudos Prospectivos , China/epidemiologia , Hipertensão/diagnóstico , Hipertensão/epidemiologia , Hipertensão/complicações , Fatores de Risco , Incidência
14.
J Nanobiotechnology ; 20(1): 509, 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36463199

RESUMO

Norcantharidin (NCTD) is a demethylated derivative of cantharidin (CTD), the main anticancer active ingredient isolated from traditional Chinese medicine Mylabris. NCTD has been approved by the State Food and Drug Administration for the treatment of various solid tumors, especially liver cancer. Although NCTD greatly reduces the toxicity of CTD, there is still a certain degree of urinary toxicity and organ toxicity, and the poor solubility, short half-life, fast metabolism, as well as high venous irritation and weak tumor targeting ability limit its widespread application in the clinic. To reduce its toxicity and improve its efficacy, design of targeted drug delivery systems based on biomaterials and nanomaterials is one of the most feasible strategies. Therefore, this review focused on the studies of targeted drug delivery systems combined with NCTD in recent years, including passive and active targeted drug delivery systems, and physicochemical targeted drug delivery systems for improving drug bioavailability and enhancing its efficacy, as well as increasing drug targeting ability and reducing its adverse effects.


Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes , Neoplasias , Estados Unidos , Sistemas de Liberação de Medicamentos , Meia-Vida , Disponibilidade Biológica , Neoplasias/tratamento farmacológico
15.
Front Psychol ; 13: 1036991, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467184

RESUMO

University phase is a major turning point in youth's life and this is a time of profound mental and cognitive development of students. Without proper direction and guidance, it is common for students to develop deviant behaviors, non-law abidance and unhealthy beliefs. In this regard, an integral part of the educational process is the imparting of moral values and law-abiding behaviors in students. The objective of this study was to explore the role of higher education system in fostering law-abiding behavior among Chinese students, as well as the issues it causes for society. For this purpose, the principles of law-abiding behavior were studied and key psychological factors used in the system were identified. The suggestions of the bibliometric research are designed to improve and expand the method for preventing student misbehavior in educational institutions, hence enhancing the efficacy of preventative work with students. They may serve as the basis for the creation and enhancement of programs and strategies aimed at teaching lawful behavior among students. More than 3,785 articles were published related law-abiding behavior from 2000 to the end of July 2022 years were examined in this research using the Scopus database and the original sample was narrowed down to include only articles, book chapter and conference papers that contributed to law-abiding behavior and higher education literature. The VOS viewer software was used to execute the descriptive statistics and scientific mapping approaches using co-citation analysis. In the descriptive analysis, we analyzed publishing patterns over time, the geographical localization of the contributing institutions, journals, the most prolific authors. The findings of the present study may also provide the foundation for a planned educational initiative whose ultimate aim is to produce a fully realized, harmonious, self-reliant, mature, and law-abiding person. The study has provided supporting evidence for how youngsters legal sensibilities are shaped in universities have been implemented. Two distinct but interdependent educational spheres, the normative legal sphere and the space for the creation and development of students' personalities must work together to raise and educate youngsters.

16.
ACS Nano ; 2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36468646

RESUMO

A key challenge for achieving continuous biosensing with existing technologies is the poor reusability of the biorecognition interface due to the difficulty in the dissociation of analytes from the bioreceptors upon surface saturation. In this work, we introduce a regeneratable biosensing scheme enabled by allosteric regulation of a re-engineered pH sensitive anti-cocaine aptamer. The aptamer can regain its affinity with target analytes due to proton-promoted duplex-to-triplex transition in DNA configuration followed by the release of adsorbed analytes. A Pd/PdHx electrode placed next to the sensor can enable the pH regulation of the local chemical environment via electrochemical reactions. Demonstration of a "flower-shaped", stretchable, and inductively coupled electronic system with sensing and energy harvesting capabilities provides a promising route to designing wireless devices in biointegrated forms. These advances have the potential for future development of electronic sensing platforms with on-chip regeneration capability for continuous, quantitative, and real-time monitoring of chemical and biological markers.

17.
Front Microbiol ; 13: 1033211, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452929

RESUMO

Talaromyces (Penicillium) marneffei (T. marneffei) is a thermally dimorphic fungus that can cause opportunistic systemic mycoses. Our previous study demonstrated that concomitant use of berberine (BBR) and fluconazole (FLC) showed a synergistic action against FLC-resistant T. marneffei (B4) in vitro. In this paper, we tried to figure out the antifungal mechanisms of BBR and FLC in T. marneffei FLC-resistant. In the microdilution test, the minimum inhibitory concentration (MIC) of FLC was 256 µg/ml before FLC and BBR combination, and was 8 µg/ml after combination, the partial inhibitory concentration index (FICI) of B4 was 0.28. After the treatments of BBR and FLC, the studies revealed that (i) increase reactive oxygen species (ROS), (ii) reduce ergosterol content, (iii) destroy the integrity of cell wall and membrane, (iv) decrease the expression of genes AtrF, MDR1, PMFCZ, and Cyp51B however ABC1 and MFS change are not obvious. These results confirmed that BBR has antifungal effect on T. marneffei, and the combination with FLC can restore the susceptibility of FLC-resistant strains to FLC, and the reduction of ergosterol content and the down-regulation of gene expression of AtrF, Mdr1, PMFCZ, and Cyp51B are the mechanisms of the antifungal effect after the combination, which provides a theoretical basis for the application of BBR in the treatment of Talaromycosis and opens up new ideas for treatment of Talaromycosis.

18.
BMC Med Genomics ; 15(1): 264, 2022 12 18.
Artigo em Inglês | MEDLINE | ID: mdl-36529725

RESUMO

Coronary artery disease (CAD) is one of the most common heart diseases, characterized by the hardening and narrowing of arteries, resisting blood supply to cardiac muscle. Despite extensive research, the pathogenesis and therapeutic options for CAD remain limited. Epigenetic regulation plays a critical role in CAD progression. Here, we report a unique DNA methylation-miRNA-mRNA regulatory network for CAD, delineated through DNA methylation assays, miRNA and mRNA sequencing, bioinformatics analyses. We also identified key signaling pathways in this network, including the miR194 promoter-miR194-MAPK signaling pathway by pyrosequencing, methylation PCR, qRT-PCR. This pathway could play a role in CAD by apoptosis. Our findings suggested that this signaling pathway may be a potential therapeutic target for CAD. We believe that our study significantly contributes to an improved understanding of the role of specific miRNAs methylation, miRNA, and mRNAs in CAD pathogenesis.


Assuntos
Doença da Artéria Coronariana , MicroRNAs , Humanos , Metilação de DNA , Doença da Artéria Coronariana/genética , Doença da Artéria Coronariana/metabolismo , Doença da Artéria Coronariana/patologia , Epigênese Genética , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
19.
Front Pharmacol ; 13: 1034870, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36532771

RESUMO

Ginseng (Panax ginseng C.A.Mey.) is the dry root and rhizome of the Araliaceae ginseng plant. It has always been used as a tonic in China for strengthening the body. Cardiovascular disease is still the main cause of death in the world. Some studies have shown that the functional components of ginseng can regulate the pathological process of various cardiovascular diseases through different mechanisms, and its formulation also plays an irreplaceable role in the clinical treatment of cardiovascular diseases. Therefore, this paper elaborates the current pharmacological effects of ginseng functional components in treating cardiovascular diseases, summarizes the adverse reactions of ginseng, and sorts out the Chinese patent medicines containing ginseng formula which can treat cardiovascular diseases.

20.
Adv Mater ; : e2210565, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36521026

RESUMO

Water electrolysis is one of the most efficient and environmentally benign methods for the hydrogen production using renewable but intermittent power sources. The well-established proton exchange membrane (PEM)-based water electrolysis, which operates under acidic conditions, possesses many advantages compared to alkaline water electrolysis, such as compact design, higher voltage efficiency and higher gas purity. However, the PEM-based water electrolysis is hampered by the low efficiency, instability and high cost of anodic electrocatalysts for the oxygen evolution reaction (OER). Many OER electrocatalysts are prone to dissolution and/or surface structure transformation under the oxidizing OER potentials in the harsh acidic environment, finally leading to a drastic decrease of catalytic performance. In this review, we comprehensively summarize, classify and discuss the recently reported acidic OER electrocatalysts. The related fundamental studies on OER mechanisms and the relationship between activity and stability are particularly highlighted in order to provide an atomistic-level understanding for the OER catalysis. A stability test protocol is suggested to evaluate the intrinsic activity degradation. We also discuss some current challenges and unresolved questions, such as the usage of carbon-based materials and the differences between the electrocatalyst performances in acidic electrolytes and PEM-based electrolyzer. Finally, the suggestions for the most promising electrocatalysts and a perspective for future research are outlined. This review presents a fresh impetus and guideline to the rational design and synthesis of high-performance acidic OER electrocatalysts for PEM-based water electrolysis. This article is protected by copyright. All rights reserved.

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