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1.
Ecotoxicol Environ Saf ; 208: 111663, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396173

RESUMO

Even after being abandoned for many years, a large number of weathered crust elution-deposited rare earth (WCED-RE) tailings continue to release ammonia nitrogen (AN) pollution into their surrounding environments. However, the influences of particle size distribution and clay minerals on AN pollution caused by these tailings have been insufficiently studied, and its causes are poorly understood. In this study, soil samples at different depths (5, 7, 9, 11 and 14 m) were collected from a rare earth tailing in Ganzhou City, Jiangxi Province, China. Particles were screened by size into six groups (2-1, 1-0.5, 0.5-0.25, 0.25-0.1, 0.1-0.075 and < 0.075 mm), and AN forms were extracted. The results showed that as soil particle size decreases, both soil specific surface area and clay content increase, leading to stronger AN enrichment ability. With increased sampling depth, the distribution of clay across the six particle fractions became more uniform, such that the accumulation of AN in soil with fine particle size was less obvious. Clay minerals with different capacities for AN enrichment vary with sampling depth. This variation is responsible for the profile of AN distribution in the mine, where AN first increases and then decreases as vertical depth is increased. Although AN content was highest at 11 m, water soluble AN content was higher in the upper part of the completely weathered layer (5 and 7 m), which poses a higher environmental risk. This study provides significant information to deepen our understanding of the distribution characteristics of AN and its main influencing factors, as well as a foundation for the prevention and remediation of nitrogen pollution from WCED-RE tailings.

2.
Biosci Rep ; 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33393595

RESUMO

The curative effect for patients with advanced gastric cancer is still unsatisfactory. Proton pump inhibitors could be a promising treatment strategy which could sensitize gastric cancer cells to antitumor drugs further, however, the underlying molecular mechanism remains to be further elucidated. In this research. it was found that omeprazole pretreatment could enhance the inhibitory effect of 5-Fu, DDP and TAX on gastric cancer cells. Interestingly, omeprazole pretreatment enhanced the total m6A level of cells due to the decreased FTO. TCGA analysis showed that FTO expression is upregulated in GC tissues and is negatively correlated with disease free survival of GC patients. It was also found that FTO inhibition induced by omeprazole enhanced the activation of mTORC1 signal pathway which inhibited the pro-survival autophagy so as to improve the antitumor efficiency of chemotherapeutic drugs on GC cells. Meanwhile, transcript level of DDIT3, which is an apoptosis-related tumor suppressor gene downstream of mTORC1 was regulated by omeprazole induced FTO silence through an m6A-dependent mechanism. This study, for the first time, found that m6A modification and its eraser FTO may play a role in the improvement of chemosensitivity mediated by proton pump inhibitor omeprazole.

3.
J Infect Dis ; 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33417695

RESUMO

BACKGROUND: Staphylococcus aureus (S. aureus) causes community- and hospital-acquired pneumonia linked to a high mortality rate. The emergence and rapid transmission of multidrug-resistant S. aureus strains have become a serious health concern, and highlight the challenges associated with the development of a vaccine to combat S. aureus pneumonia. METHODS: This study evaluated the effects of intrapulmonary (i.pulmon.) immunization on the immune response and protection against S. aureus lung infection in a respiratory mouse model using a subunit vaccine. RESULTS: Compared with the intranasal immunized mice, the i.pulmon. immunized mice had lower levels of pulmonary bacterial colonization and lethality, accompanied by alleviated lung inflammation with reduced pro-inflammatory cytokines and increased levels of interleukin-10 and antimicrobial peptide following i.pulmon. challenge. Optimal protection was associated with increased pulmonary antibodies and resident memory T cells. Moreover, i.pulmon. immunization provided long-lasting pulmonary protection for at least 6 months, with persistent cellular and humoral immunity in the lungs. CONCLUSIONS: Vaccine reaching the deep lung by i.pulmon. immunization plays a significant role in the induction of efficacious and long-lasting immunity against S. aureus in the lung parenchyma. Hence, i.pulmon. immunization can be a strategy for the development of a vaccine against S. aureus pneumonia.

4.
Oncogene ; 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33420374

RESUMO

Arginine methylation is an important posttranslational modification catalyzed by protein arginine methyltransferases (PRMTs). However, the role of PRMTs in colorectal cancer (CRC) progression is not well understood. Here we report that non-POU domain-containing octamer-binding protein (NONO) is overexpressed in CRC tissue and is a potential marker for poor prognosis in CRC patients. NONO silencing resulted in decreased proliferation, migration, and invasion of CRC cells, whereas overexpression had the opposite effect. In a xenograft model, tumors derived from NONO-deficient CRC cells were smaller than those derived from wild-type (WT) cells, and PRMT1 inhibition blocked CRC xenograft progression. A mass spectrometry analysis indicated that NONO is a substrate of PRMT1. R251 of NONO was asymmetrically dimethylated by PRMT1 in vitro and in vivo. Compared to NONO WT cells, NONO R251K mutant-expressing CRC cells showed reduced proliferation, migration, and invasion, and PRMT1 knockdown or pharmacological inhibition abrogated the malignant phenotype associated with NONO asymmetric dimethylation in both KRAS WT and mutant CRC cells. Compared to adjacent normal tissue, PRMT1 was highly expressed in the CRC zone in clinical specimens, which was correlated with poor overall survival in patients with locally advanced CRC. These results demonstrate that PRMT1-mediated methylation of NONO at R251 promotes CRC growth and metastasis, and suggest that PRMT1 inhibition may be an effective therapeutic strategy for CRC treatment regardless of KRAS mutation status.

5.
BMC Musculoskelet Disord ; 22(1): 34, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33413256

RESUMO

BACKGROUND: Large post-traumatic tibial bone defects combined with soft tissue defects are a common orthopedic clinical problem associated with poor outcomes when treated using traditional surgical methods. The study was designed to investigate the safety and efficacy of trifocal bone transport (TFT) and soft-tissue transport with the Ilizarov technique for large posttraumatic tibial bone and soft tissue defects. METHODS: We retrospectively reviewed 31 patients with massive posttraumatic tibial bone and soft tissue defects from May 2009 to May 2016. All of the eligible patients were managed by TFT and soft-tissue transport. The median age was 33.4 years (range, 2-58 years). The mean defect of bone was 11.87 cm ± 2.78 cm (range, 8.2-18.2 cm) after radical resection performed by TFT. The soft tissue defects ranged from 7 cm × 8 cm to 24 cm × 12 cm. The observed results included bone union time, wound close time and true complications. The Association for the Study and Application of the Method of Ilizarov (ASAMI) scoring system was used to assess bone and functional results and postoperative complications were evaluated by Paley classification. RESULTS: The mean duration of follow-up after frame removal was 32 months (range, 12-96 months). All cases achieved complete union in both the elongation sites and the docking sites, and eradication of infection. The mean bone transport time was 94.04 ± 23.33 days (range, 63.7-147 days). The mean external fixation time was 22.74 ± 6.82 months (range, 14-37 months), and the mean external fixation index (EFI) was 1.91 ± 0.3 months/cm (range, 1.2-2.5 months/cm). The bone results were excellent in 6 patients, good in 14 patients, fair in 8 patients and poor in 3 patients. The functional results were excellent in 8 patients, good in 15 patients, fair in 5 patients and poor in 3 patients. CONCLUSION: TFT, in conjunction with soft tissue transport technique, can give good results in most patients (in this article, good and excellent results were observed in 64% of patients). Soft tissue transport is a feasible method in providing good soft tissue coverage on the bone ends. Although it has no advantages over microvascular techniques, it might be an good alternative in the absence of an experienced flap surgeon. Nonetheless, high-quality controlled studies are needed to assess its long-term safety and efficacy.

6.
Am J Hum Genet ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33434492

RESUMO

Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) is associated with congenital absence of the uterus, cervix, and the upper part of the vagina; it is a sex-limited trait. Disrupted development of the Müllerian ducts (MD)/Wölffian ducts (WD) through multifactorial mechanisms has been proposed to underlie MRKHS. In this study, exome sequencing (ES) was performed on a Chinese discovery cohort (442 affected subjects and 941 female control subjects) and a replication MRKHS cohort (150 affected subjects of mixed ethnicity from North America, South America, and Europe). Phenotypic follow-up of the female reproductive system was performed on an additional cohort of PAX8-associated congenital hypothyroidism (CH) (n = 5, Chinese). By analyzing 19 candidate genes essential for MD/WD development, we identified 12 likely gene-disrupting (LGD) variants in 7 genes: PAX8 (n = 4), BMP4 (n = 2), BMP7 (n = 2), TBX6 (n = 1), HOXA10 (n = 1), EMX2 (n = 1), and WNT9B (n = 1), while LGD variants in these genes were not detected in control samples (p = 1.27E-06). Interestingly, a sex-limited penetrance with paternal inheritance was observed in multiple families. One additional PAX8 LGD variant from the replication cohort and two missense variants from both cohorts were revealed to cause loss-of-function of the protein. From the PAX8-associated CH cohort, we identified one individual presenting a syndromic condition characterized by CH and MRKHS (CH-MRKHS). Our study demonstrates the comprehensive utilization of knowledge from developmental biology toward elucidating genetic perturbations, i.e., rare pathogenic alleles involving the same loci, contributing to human birth defects.

7.
Ying Yong Sheng Tai Xue Bao ; 32(1): 191-200, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33477227

RESUMO

Based on a long-term experiment in the Changwu Agro-ecological Experimental Station in Xianyang, Shaanxi, China, we examined the effects of fertilization and planting patterns on soil aggregate quantity, aggregate stability and total carbon and organic carbon distribution in different aggregate fractions through dry and wet sieving methods, as well as the TOC combustion method. There were ten treatments, including uncultivated (R), wheat continuous cropping (CK/W), wheat-corn rotation (L), and nitrogen fertilizer (N), phosphorus fertilizer (P), nitrogen and phosphorus fertilizer (NP), organic fertilizer (M), nitrogen and organic fertilizer (NM), phosphorus and organic fertilizer (PM), nitrogen and phosphorus and organic fertilizer (NPM) for CK/W. The results showed that fertilizer application and planting patterns affected soil aggregate distribution and stability, the contents and contribution rates of total C and organic C. Force-stable aggregate was mainly constituted by >0.25 mm aggregate (>67%), which was reduced by fertilization. Continuous cropping decreased micro-aggregate while rotation facilitated it and the effect was larger than fertilization. Water-stable aggregate was mainly comprised of micro-aggregate (<0.25 mm), the contribution of which was larger than 61%. Both fertilizer application and planting pattern reduced water-stable micro-aggregate. Fertilizer application and planting pattern decreased the percentage of aggregate destruction rate (PAD) and increased macro-aggregate (>0.25 mm, R0.25) content. Organic fertilizer significantly improved total C and organic C concentrations in all the fractions of force-stable aggregates. Continuous cropping and rotation cropping increased total C concentration in all the aggregate fractions while rotation cropping significantly decreased organic C concentration. Single N and P fertilization decreased soil total C concentration, while mixed application of N and P fertilizers, and organic fertilizer significantly increased soil total C concentration. The effect of planting patterns on soil total C was lower than that of fertilization. Both continuous cropping and rotation cropping increased soil total C. Mixed application of N and P fertilizers, and organic fertilizer signifi-cantly increased soil organic C concentration while single N and P fertilization decreased it. The effect of planting patterns on soil organic C was lower than that of fertilization, while rotation cropping did not facilitate soil organic C. Micro-aggregate was the most notable size fraction to total carbon and organic C, with the contribution being 21.2%-33.6%. Fertilization and planting pattern increased the contribution rate of micro-aggregate in soil total C. NP and NPM significantly increased the contribution rate of micro-aggregate in soil total C and soil organic C. The effect of rotation cropping was most obvious in driving the contribution rate of micro-aggregate in soil total C and soil organic C.

8.
ACS Chem Neurosci ; 12(1): 244-255, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33356131

RESUMO

The protein-protein interaction between neuronal nitric oxide syntheses (nNOS) and the carboxy-terminal PDZ ligand of nNOS (CAPON) is a potential target for the treatment of ischemic stroke. Our previous study had identified ZLc-002 as a promising lead compound for inhibiting nNOS-CAPON coupling. To find better neuroprotective agents disrupting the ischemia-induced nNOS-CAPON interaction, a series of N-cyclohexylethyl-[A/G]-[D/E]-X-V peptides based on the carboxy-terminal tetrapeptide of CAPON was designed, synthesized, and evaluated in this study. Herein, we reported an affinity-based fluorescence polarization (FP) method using 5-carboxyfluorescein (5-FAM) labeled CAPON (496-506) peptide as the probe for high-throughput screening of the small-molecule inhibitors of the PDZ domain of nNOS. N-Cyclohexylethyl-ADAV displayed the most potent affinity for the nNOS PDZ domain in the FP and isothermal titration calorimetry (ITC) (ΔH = -1670 ± 151.0 cal/mol) assays. To improve bioavailability, lipophilicity, and membrane permeability, the Asp methylation was employed to get N-cyclohexylethyl-AD(OMe)AV, which possesses good blood-brain barrier (BBB) permeability in vitro parallel artificial membrane permeability assay (PAMPA)-BBB (Pe = 6.07 cm/s) and in vivo assays. In addition, N-cyclohexylethyl-AD(OMe)AV (10 mg/kg body weight, i.v., immediately after reperfusion) substantially reduced infarct size in rats, which was measured 24 h after reperfusion and subjected to 120 min of middle cerebral artery occlusion (MCAO).

9.
Chemosphere ; 262: 127968, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182104

RESUMO

Electrocoagulation (EC) coupled with E-peroxone process (ECP) was a promising and cost-effective integrated technology for shale gas fracturing flowback water (SGFFW) treatment. In this study, the ECP process was comprehensively compared with two sequential processes (EC followed by E-peroxone (EC-E-peroxone) and E-peroxone followed by EC (E-peroxone-EC)) to elucidate the synergistic effect of this coupled process. In EC-E-peroxone process, COD decreased by 89.2%, while COD decreased by 82.5% for 180 min in E-peroxone-EC process. However, COD removal efficiency was 82.4% in ECP for only 90 min. Average current efficiency of the ECP process was 29.9%, which was twice than that of the sequential processes. The enhancement factor was calculated to be 1.63, demonstrating the substantial significant synergistic effects for ECP. Only low MW components could be observed for the EC-E-peroxone (average MW = 533 Da with PD ≈ 1) and ECP process (MW = 538 Da with PD ≈ 1). These results suggested that EC-E-peroxone and ECP process had much stronger oxidation ability, demonstrating the enhancement of OH production induced by the Al-based coagulants might be responsible for the significant enhancement of COD removal. These results indicated there could be a synergistic effect between EC and ozone in addition to EC and E-peroxone reactions. Compared to the two sequential processes, ECP was a high efficiency and space-saving electrochemical system with simultaneous coagulation and enhanced OH generation by the products of anode and the cathode.


Assuntos
Técnicas Eletroquímicas/métodos , Peróxido de Hidrogênio/química , Gás Natural/análise , Ozônio/química , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Análise da Demanda Biológica de Oxigênio , China , Eletrodos , Floculação , Fraturamento Hidráulico , Oxirredução , Águas Residuárias/química
10.
Artigo em Inglês | MEDLINE | ID: mdl-33315564

RESUMO

Image fusion plays a critical role in a variety of vision and learning applications. Current fusion approaches are designed to characterize source images, focusing on a certain type of fusion task while limited in a wide scenario. Moreover, other fusion strategies (i.e., weighted averaging, choose-max) cannot undertake the challenging fusion tasks, which furthermore leads to undesirable artifacts facilely emerged in their fused results. In this paper, we propose a generic image fusion method with a bilevel optimization paradigm, targeting on multi-modality image fusion tasks. Corresponding alternation optimization is conducted on certain components decoupled from source images. Via adaptive integration weight maps, we are able to get the flexible fusion strategy across multi-modality images. We successfully applied it to three types of image fusion tasks, including infrared and visible, computed tomography and magnetic resonance imaging, and magnetic resonance imaging and single-photon emission computed tomography image fusion. Results highlight the performance and versatility of our approach from both quantitative and qualitative aspects.

11.
Diabetes Res Clin Pract ; 171: 108623, 2020 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-33316314

RESUMO

AIMS: To evaluate the influence of gestational diabetes mellitus (GDM) on the perinatal outcomes of twin pregnancies and its impact on fetal growth profiles of twin offspring from 6 weeks to 12 months of corrected age. METHODS: A longitudinal cohort study was conducted among pregnant women with twins and their twin offspring. All information on perinatal outcomes and child growth trajectories from 6 weeks to 12 months of corrected age were obtained and analyzed using a general linear model and logistic regression models. RESULTS: GDM was not correlated with adverse perinatal outcomes of twin pregnancies; however, in monochorionic diamniotic (MCDA), but not dichorionic diamniotic (DCDA) twin pregnancies, GDM was correlated with gestational hypertension disorder and a fetus being small for gestational age (OR, 2.68; 95% CI 1.16-6.04 and OR, 0.35; 95% CI 0.16-0.76, respectively). In both MCDA and DCDA groups, GDM was positively associated with a higher risk of childhood overweight at 6 months of corrected age (2.32 [1.05, 5.09] and 2.00 [1.13, 3.53]). CONCLUSIONS: GDM had a greater impact on MCDA twin pregnancies in terms of maternal gestational hypertension disease and small for gestational age of newborns. Additionally, twin offspring exposed to GDM had a higher risk of being overweight at 6 months of corrected age irrespective of chorionicity. CLINICAL TRIAL REGISTRATION: ChiCTR-OOC-16008203.

12.
Int J Mol Sci ; 21(24)2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33339363

RESUMO

Lunasin is a soybean-derived peptide that exhibits anticancer bioactivity in different cancer cells and has been identified in different plants. However, recent studies revealed through molecular and chemical analyses that lunasin was absent in wheat and other cereals. In this study, the soybean-derived lunasin was cloned into pCAMBIA3300 and we transferred the expression vector into wheat via an Agrobacterium-mediated transformation. The identification of transgenic wheat was detected by polymerase chain reaction, Western blot analysis, and ultra-performance liquid chromatography with tandem mass spectrometry. An enzyme-linked immunosorbent assay showed that lunasin content in transgenic wheat L32-3, L32-6, and L33-1 was 308.63, 436.78, and 349.07 µg/g, respectively, while lunasin was not detected in wild-type wheat. Lunasin enrichment from transgenic wheat displayed an increased anti-proliferative activity compared with peptide enrichment from wild-type wheat in HT-29 cells. Moreover, the results of a real-time quantitative polymerase chain reaction showed a significant elevation in p21, Bax, and caspase-3 expression, while Bcl-2 was significantly downregulated. In conclusion, soybean-derived lunasin was successfully expressed in wheat via Agrobacterium-mediated transformation and may exert anti-proliferative activity by regulating the apoptosis pathway in HT-29 cells, which provides an effective approach to compensate for the absence of lunasin in wheat.

13.
Front Cell Dev Biol ; 8: 543856, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33195186

RESUMO

Background: Cancer stem cells (CSCs), responsible for cancer metastasis and recurrence, are generated from non-CSCs after chemo-radiation therapy. This study investigated the induction of CSC potential in non-stem breast cancer cells and the underlying molecular mechanisms in detachment culture. Methods: Bulk breast cancer cells, or sorted non-CSCs and CSCs were cultured under an attached or detached condition to assess CSC numbers, ability to form tumor spheres, expression of stemness markers, and chemoresistance. Lentivirus carrying CD147 shRNA or cDNA was used to manipulate CD147 expression, while CD147 ligand recombinant cyclophilin A (CyPA) or its inhibitor was used to activate or inhibit CD147 signaling. Results: Detachment promoted anoikis resistance, chemoresistance, sphere formation, self-renewal, and expression of stemness markers in breast cancer cells. Detachment increased functional ALDH+ or CD44highCD24-/low CSCs, and induced CSC potential in ALDH- or CD44 low CD24high non-CSCs. Upon detachment, both CD147 expression and CyPA secretion were enhanced, and CyPA-CD147 activation mediated detachment induced CSC potential in non-CSCs via STAT3 signaling. Clinically, CD147 and pSTAT3 were highly co-expressed and correlated with poor overall survival and tumor recurrence in breast cancer patients. Conclusion: This study demonstrates that detachment induces the generation of CSCs from non-stem breast cancer cells via CyPA-CD147 signaling, indicating that targeting CD147 may serve as a potential novel therapeutic strategy for lethal metastatic breast cancer by eliminating induced CSCs.

14.
iScience ; 23(12): 101767, 2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33251497

RESUMO

Bacterial coinfection is a major cause of influenza-associated mortality. Most people have experienced infections with bacterial pathogens commonly associated with influenza A virus (IAV) coinfection before IAV exposure; however, bacterial clearance through the immunological memory response (IMR) in coinfected patients is inefficient, suggesting that the IMR to bacteria is impaired during IAV infection. Adoptive transfer of CD4+ T cells from mice that had experienced bacterial infection into IAV-infected mice revealed that memory protection against bacteria was weakened in the latter. Additionally, memory Th17 cell responses were impaired due to an IFN-γ-dependent reduction in Th17 cell proliferation and delayed migration of CD4+ T cells into the lungs. A bacterium-specific antibody-mediated memory response was also substantially reduced in coinfected mice, independently of IFN-γ. These findings provide additional perspectives on the pathogenesis of coinfection and suggest additional strategies for the treatment of defective antibacterial immunity and the design of bacterial vaccines against coinfection.

15.
J Bioenerg Biomembr ; 52(6): 441-451, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33196964

RESUMO

Nucleolar and spindle-associated protein 1 (NUSAP1) is a pivotal tumor-related protein that has been implicated in the progression of broad spectrum of tumors. However, no detailed study of the role of NUSAP1 in nasopharyngeal carcinoma (NPC) has been reported. The aim of this work is to enhance our understanding of NUSAP1 in the progression of NPC. By analyzing data available within the Oncomine database, we found that NUSAP1 expression was elevated in NPC relative to normal tissues. Further, we showed that NUSAP1 expression in clinical specimens of NPC and several NPC cell lines was elevated. Down-regulation of NUSAP1 by gene silencing markedly depleted the capacity of NPC cells to proliferate and invade. Contrastingly, overexpression of NUSAP1 potentiated the proliferative and invasive abilities of NPC cells. Further mechanistic research revealed that NUSAP1 knockdown decreased levels of Wnt/ß-catenin signaling in NPC cells via a mechanism associated with downregulation of glycogen synthase kinase-3ß (GSK-3ß) phosphorylation. However, suppression of GSK-3ß markedly abolished the inhibitory effect of NUSAP1 knockdown on Wnt/ß-catenin signaling. Further, inhibition of Wnt/ß-catenin signaling partially reversed NUSAP1-mediated tumor growth in NPC cells. In addition, NUSAP1 knockdown restrained tumorigenesis of NPC in vivo, and was associated with down-regulation of Wnt/ß-catenin signaling. In conclusion, these findings demonstrate that NUSAP1 is capable of accelerating proliferation and invasion in NPC cells by potentiating Wnt/ß-catenin signaling. Our study unveils a potential role of NUSAP1 in promoting NPC tumors and suggests that the protein is an attractive antitumor target for NPC treatment.

16.
Artigo em Chinês | MEDLINE | ID: mdl-33254354

RESUMO

Cingulate cortex, as an important part of limbic system, is connected with number of areas in the brain, which regulate and control the conduction of multiple sensations. Studies of tinnitus have shown that abnormal changes in cingulate cortex are involved in the process of tinnitus, and play a key role in noise cancelling, cognition and emotional experience of tinnitus. This paper reviews the physiological function of the cingulate cortex and its role in the mechanism of tinnitus, providing new ideas for the treatment of tinnitus.

17.
BMC Cardiovasc Disord ; 20(1): 490, 2020 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-33213382

RESUMO

BACKGROUND: Estimated glomerular filtration rate (eGFR) is a widely accepted indicator of renal function. The aim of this study was to evaluate the relationship between eGFR and 3-year clinical outcomes among Chinese patients with atrial fibrillation (AF). METHODS: We retrospectively studied 433 consecutive Chinese patients with AF (51.0% males, mean age 65.6 ± 13.2 years) between February 2013 and December 2017. Baseline clinical data were collected according to medical records. eGFR was calculated by MDRD equation for Chinese patients according to baseline age, sex and serum creatinine. The primary clinical outcome of interest was all-cause mortality. RESULTS: During a median follow-up period of 3.1 (0.5-4.5) years, 73 deaths (16.9%) were recorded. Multivariate Cox regression analyses indicated that eGFR was independently associated with all-cause death in total population [hazard ratio (HR) 0.984; 95% confidence interval (CI) 0.972-0.995, P = 0.006] and patients free of valvular heart diseases (VHDs) (HR 0.975; 95% CI 0.959-0.992, P = 0.003), but not with VHDs. A receiver operating characteristic (ROC) analysis revealed that reduced eGFR predicted all-cause mortality with areas under the ROC curve of 0.637 (95% CI 0.539-0.735, P = 0.004) in AF patients free of VHDs. CONCLUSIONS: eGFR is an independent predictor of 3-year all-cause mortality among Chinese patients with AF, especially among those patients free of VHDs.

18.
Jpn J Infect Dis ; 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33250493

RESUMO

Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. Persistent infection is associated with the development of cervical cancer. Men could serve as a medium for HPV transmission among women, and little is known about the prevalence and genotypes of HPV infection among men in Yunnan province. This study is designed for investigating these parameters among men in this region. Clinical information was collected from January 2015 to May 2020, and a total of 369 men who sought to get tested for HPV were included in this study. HPV screening was performed using the HPV GenoArray Test Kit and the anatomical site of sample collection is urethra. The mean age was 36.15 ± 9.08 years. Our results revealed that the prevalence of HPV infection among men was 23.8% in Yunnan Province, and there was no association between age and HPV prevalence in men. There were 72 (81.81%) individuals positive for a single HPV type and 16 (18.19%) positive for multiple types. The highest prevalent oncogenic HPV types were types 52, 51, and 16. The prevalence of HPV in men was relatively high, and the most prevalent types of HPV infection were similar between men and women from this region.

19.
World J Emerg Med ; 11(4): 246-254, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33014221

RESUMO

BACKGROUND: Disturbance of mitochondrial fission and fusion (termed mitochondrial dynamics) is one of the leading causes of ischemia/reperfusion (I/R)-induced myocardial injury. Previous studies showed that mitochondrial aldehyde dehydrogenase 2 (ALDH2) conferred cardioprotective effect against myocardial I/R injury and suppressed I/R-induced excessive mitophagy in cardiomyocytes. However, whether ALDH2 participates in the regulation of mitochondrial dynamics during myocardial I/R injury remains unknown. METHODS: In the present study, we investigated the effect of ALDH2 on mitochondrial dynamics and the underlying mechanisms using the H9c2 cells exposed to hypoxia/reoxygenation (H/R) as an in vitro model of myocardial I/R injury. RESULTS: Cardiomyocyte apoptosis was significantly increased after oxygen-glucose deprivation and reoxygenation (OGD/R), and ALDH2 activation largely decreased the cardiomyocyte apoptosis. Additionally, we found that both ALDH2 activation and overexpression significantly inhibited the increased mitochondrial fission after OGD/R. Furthermore, we found that ALDH2 dominantly suppressed dynamin-related protein 1 (Drp1) phosphorylation (Ser616) and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation (Thr172) but not interfered with the expression levels of mitochondrial shaping proteins. CONCLUSIONS: We demonstrate the protective effect of ALDH2 against cardiomyocyte H/R injury with a novel mechanism on mitochondrial fission/fusion.

20.
Adv Mater ; : e2003065, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33124725

RESUMO

Nanomaterial-based enzyme-mimetic catalysts (Enz-Cats) have received considerable attention because of their optimized and enhanced catalytic performances and selectivities in diverse physiological environments compared with natural enzymes. Recently, owing to their molecular/atomic-level catalytic centers, high porosity, large surface area, high loading capacity, and homogeneous structure, metal-organic frameworks (MOFs) have emerged as one of the most promising materials in engineering Enz-Cats. Here, the recent advances in the design of MOF-engineered Enz-Cats, including their preparation methods, composite constructions, structural characterizations, and biomedical applications, are highlighted and commented upon. In particular, the performance, selectivities, essential mechanisms, and potential structure-property relations of these MOF-engineered Enz-Cats in accelerating catalytic reactions are discussed. Some potential biomedical applications of these MOF-engineered Enz-Cats are also breifly proposed. These applications include, for example, tumor therapies, bacterial disinfection, tissue regeneration, and biosensors. Finally, the future opportunities and challenges in emerging research frontiers are thoroughly discussed. Thereby, potential pathways and perspectives for designing future state-of-the-art Enz-Cats in biomedical sciences are offered.

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