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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(4): 366-371, 2022 Apr 15.
Artigo em Chinês | MEDLINE | ID: mdl-35527409

RESUMO

OBJECTIVES: To investigate the influencing factors for the quality of bowel preparation before colonoscopy in children and the association of the interval from the last administration of laxative to the start of colonoscopy (shortly referred to as waiting time) with the quality of bowel preparation. METHODS: A retrospective analysis was performed for the children who were admitted to the Department of Gastroenterology, Children's Hospital of Nanjing Medical University, from January to November 2020, and received bowel preparation with polyethylene glycol electrolyte powder combined with diet control before colonoscopy. According to the score of Boston bowel preparation scale, they were divided into two groups: adequate bowel preparation group (n=337) and inadequate bowel preparation group (n=30). Related data were collected from the children in both groups, including general information, possible influencing factors for the quality of bowel preparation, adverse reactions associated with bowel preparation, duration of colonoscopy, and postoperative diagnosis. Univariate and multivariate analyses were used to explore the influencing factors for the quality of bowel preparation. RESULTS: The univariate analysis showed that age, body weight, and waiting time were associated with inadequate bowel preparation (P<0.05). The multivariate analysis showed that older age (OR=2.155, 95%CI: 1.087-4.273, P=0.028) and longer waiting time (OR=1.559, 95% CI: 1.191-2.041, P=0.001) were independent risk factors for inadequate bowel preparation. The receiver operating characteristic (ROC) curve analysis showed that the cut-off value of waiting time was 5.5 hours in determining whether bowel preparation was adequate or not, with a sensitivity of 90.0%, a specificity of 50.7%, and an area under the ROC curve of 0.708. After grouping based on waiting time, it was found that the incidence rate of inadequate bowel preparation in the ≥5.5 hours group was significantly higher than that in the <5.5 hours group [14.0% (27/193) vs 1.7% (3/174), P<0.001]. CONCLUSIONS: For children who use polyethylene glycol electrolyte powder combined with diet control for bowel preparation, older age is an independent risk factor for inadequate bowel preparation before colonoscopy, which may be associated with an insufficient dose of polyethylene glycol in older children. Longer waiting time is also an independent risk factor for inadequate bowel preparation, and it is recommended that the waiting time should not exceed 5.5 hours.


Assuntos
Catárticos , Colonoscopia , Criança , Dieta , Eletrólitos , Humanos , Polietilenoglicóis/efeitos adversos , Pós , Estudos Retrospectivos
2.
J Chromatogr A ; 1673: 463082, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35490466

RESUMO

In this paper, a micro gas chromatographic column (µGCC) was fabricated via the layer-by-layer (LBL) deposition of mesoporous silica (MS) as the stationary phase. The µGCC with embedded elliptical-cylindrical posts was fabricated by typical micro-electro-mechanical system (MEMS) techniques. The MS powder was prepared by a sol-gel method and then deposited inside the microchannels of the µGCC by LBL deposition. This process avoided the high temperature required for the sol-gel method to directly prepare MS inside the microchannel surfaces of the µGCC. Mixtures of light alkanes (C1-C4) could be separated in the µGCC. The separation results showed that the resolution between the components of the sample increased with the number of deposition cycles. When the number of deposition cycles increased from 1 to 8, the resolution of methane and ethane was increased from unseparated to 5.89.

3.
Photoacoustics ; 26: 100360, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35574187

RESUMO

Acoustic-resolution photoacoustic microscopy (AR-PAM) image resolution is determined by the point spread function (PSF) of the imaging system. Previous algorithms, including Richardson-Lucy (R-L) deconvolution and model-based (MB) deconvolution, improve spatial resolution by taking advantage of the PSF as prior knowledge. However, these methods encounter the problems of inaccurate deconvolution, meaning the deconvolved feature size and the original one are not consistent (e.g., the former can be smaller than the latter). We present a novel deep convolution neural network (CNN)-based algorithm featuring high-fidelity recovery of multiscale feature size to improve lateral resolution of AR-PAM. The CNN is trained with simulated image pairs of line patterns, which is to mimic blood vessels. To investigate the suitable CNN model structure and elaborate on the effectiveness of CNN methods compared with non-learning methods, we select five different CNN models, while R-L and directional MB methods are also applied for comparison. Besides simulated data, experimental data including tungsten wires, leaf veins, and in vivo blood vessels are also evaluated. A custom-defined metric of relative size error (RSE) is used to quantify the multiscale feature recovery ability of different methods. Compared to other methods, enhanced deep super resolution (EDSR) network and residual in residual dense block network (RRDBNet) model show better recovery in terms of RSE for tungsten wires with diameters ranging from 30 µ m to 120 µ m . Moreover, AR-PAM images of leaf veins are tested to demonstrate the effectiveness of the optimized CNN methods (by EDSR and RRDBNet) for complex patterns. Finally, in vivo images of mouse ear blood vessels and rat ear blood vessels are acquired and then deconvolved, and the results show that the proposed CNN method (notably RRDBNet) enables accurate deconvolution of multiscale feature size and thus good fidelity.

4.
Front Psychiatry ; 13: 837399, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35392382

RESUMO

Purpose: The purpose of the study was to determine the long-term effects of one-week self-guided internet cognitive behavioral treatments for insomnia (CBTI) on situational insomnia during the COVID-19 pandemic. Patients and Methods: The participants with situational insomnia (n = 194) were recruited from March 2020 to April 2020 in Guangzhou, China. The insomnia severity index (ISI), pre-sleep arousal scale (PSAS), and hospital anxiety and depression scale (HADS) were evaluated at baseline and a one-week internet CBTI program was delivered to all individuals. The participants were divided into the complete treatment group (the participants completed all seven modules of the CBTI course, n = 75), and the incomplete treatment group (the participants completed 0-6 modules of the CBTI course, n = 119). A total of 135 participants completed the post-intervention assessments. At 3 months follow-up, a total of 117 participants (complete treatment group: n = 51; incomplete treatment group: n = 66) completed the assessments of the ISI, PSAS and HADS. The transition rate from situational insomnia to chronic insomnia (duration of insomnia ≥ 3 months and ISI ≥ 8) was calculated in the two groups. Linear mixed effect model was used to investigate the effect of group (between the two groups), time (baseline vs. follow-up), and interaction (group x time) on various questionnaire score. Results: The transition rate from situational insomnia to chronic insomnia was significantly lower in the complete treatment group compared to the incomplete treatment group (27.5%, 14/51 vs. 48.5%, 32/66, p = 0.023). There were significant differences in group effect (p = 0.032), time effect (p = 0.000) and group × time effect (p = 0.048) between the two groups in the ISI total score. The ISI total scores decreased in both groups during follow-up compared to their baseline values, with a greater magnitude of decrease in the complete treatment group. There were no significant group x time effects between the two groups in the PSAS-total score, PSAS-somatic, PSAS-cognitive score, HADS total score, HADS anxiety score or HADS depression score. Conclusion: Our results suggested that one-week self-guided internet CBTI prevented the development of chronic insomnia from situational insomnia during the COVID-19 pandemic.

5.
Stem Cells Int ; 2022: 9977610, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35283996

RESUMO

Our previous study found that incubating mesenchymal stem cells (MSC) with tanshinone IIA (TIIA) before transplantation could significantly increase the inhibitory effect of MSC on neuroinflammation. Here, we investigated the possible mechanism of this effect. N9 cells and MSC were inoculated at a ratio of 1 : 1 into a Transwell coculture system. MSC were inoculated into the upper chamber, and N9 cells were inoculated into the lower chamber. In this experiment, N9 cells were treated with 1 µg/mL lipopolysaccharide (LPS) for 24 hours to induce inflammation, MSC were treated with 10 µM TIIA for 48 hours to prepare TIIA-incubated MSC (TIIA-MSC), and TREM2 siRNA was used to silence the TREM2 gene in MSC. The changes in IL-1ß, IL-6, and TNF-α were evaluated by Western blotting. We found that LPS significantly increased the levels of IL-1ß, IL-6, and TNF-α. While both MSC and TIIA-MSC downregulated the levels of (P = 0.092, P = 0.002), IL-6 (P = 0.014, P < 0.001), and TNF-α (P = 0.044, P = 0.003), TIIA-MSC downregulated IL-6 more significantly (P = 0.026). In addition, silencing TREM2 reduced the ability of TIIA-MSC to attenuate IL-6 (P = 0.005) and TNF-α (P = 0.033). These data suggest that the enhanced anti-inflammatory effect of TIIA-MSC on LPS-induced N9 cells may be mediated through the TREM2 signaling pathway.

6.
Biomed Opt Express ; 13(2): 1026-1044, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-35284174

RESUMO

By considering the line pattern of acoustic-resolution photoacoustic microscopy (AR-PAM) vessel images, we develop modified algorithms for synthetic aperture focusing technique (SAFT) and deconvolution based on a directional approach to enhance images. The modified algorithms consist of Fourier accumulation SAFT (FA-SAFT) and directional model-based (D-MB) deconvolution. To evaluate the performance of our algorithms, we conduct a series of imaging experiments and apply our algorithms, and existing SAFT and deconvolution algorithms are also applied for side-by-side comparison. By imaging tungsten wire phantom, our algorithms enable full width at half maximum of 26 - 31 µm over depth of focus of 1.8 mm and minimum resolvable distance of 46 - 49 µm, besting existing SAFT and deconvolution algorithms. Imaging of leaf skeleton phantom and in vivo imaging of mouse blood vessels also prove that our algorithm is capable of providing high-resolution, high-signal-to-noise ratio, and good-fidelity results for complex structures and for in vivo applications, especially for the images with the line pattern. The proposed directional approach can not only be used in AR-PAM but also in other imaging modalities to deal with the line pattern, such as FA-SAFT for ultrasound imaging and D-MB deconvolution for optical coherence tomography angiography.

9.
Sci Rep ; 12(1): 4940, 2022 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-35322023

RESUMO

Evidences indicate that elevated levels of circulating ErbB2 are closely associated with increased incidence of diabetes. However, the relationship between ErbB2 concentration and glycemic variations (GV) in type 2 diabetic (T2D) patients remains elucidated. The aim of this study was to assess whether there is an association between serum ErbB2 concentration and GV in newly diagnosed T2D patients. This was a three-center, and observational study. Between April 2019 and July 2019, a total of 106 newly diagnosed T2D patients were recruited. All recruited subjects were admitted as inpatients and received anti-diabetes agents free during the study period. At baseline, fasting serum was collected for ErbB2 measurement and all recruited patients were subjected a prospective CGM for at least 3 days. The primary endpoint was the relationships between ErbB2 concentrations and GV in T2D patients. Data of a total of 95 subjects who met the inclusion criteria were analyzed at the endpoint. Subjects were divided into quartiles according to their serum ErbB2 concentrations. We observed that subjects with an elevated level of ErbB2 had a higher value of GV in terms of mean amplitude of glucose excursion (MAGE), standard deviation of mean glucose (SDMG), and the coefficient of variation (CV%) than those with lower levels (all P < 0.05). Multiple linear regression analyzes after adjusting for confounder factors indicate that serum ErbB2 levels were significantly positively correlated with the MAGE (ß = 0.664, t = 7.218, P < 0.01), SD (ß = 0.469, t = 5.125, P < 0.01) and CV% (ß = 0.337, t = 4.442, P < 0.01), respectively. Our data indicated that diabetic patients with higher ErbB2 concentrations may have large GV, which is an independent risk factor for microvascular and macrovascular complications.


Assuntos
Automonitorização da Glicemia , Diabetes Mellitus Tipo 2 , Glicemia , Diabetes Mellitus Tipo 2/complicações , Hemoglobina A Glicada/análise , Humanos , Estudos Prospectivos , Receptor ErbB-2
10.
J Orthop Surg Res ; 17(1): 100, 2022 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-35172868

RESUMO

OBJECTIVE: Surgical treatment of lumbar spinal stenosis (LSS) in octogenarians (patients aged ≥ 80 years) has been a challenge. Inter-spinal distraction fusion (ISDF)-a minimally invasive procedure-was used for treating LSS in octogenarians. This retrospective cohort study aimed to investigate the clinical efficacy and safety of a minimally invasive ISDF technique for LSS in octogenarian patients. METHODS: From April 2015 to April 2019, octogenarian patients who underwent lumbar fusion surgery due to single-segment LSS were included. The patients were grouped into the ISDF group and posterior lumbar interbody fusion (PLIF) group based on the type of surgery. Clinical outcomes were evaluated using scores of the visual analog pain scale (VAS), Oswestry Disability Index (ODI), and Japanese Orthopedics Association (JOA) scale. Radiographs were assessed for the intervertebral angle (IA), lumbar lordosis (LL), and posterior disc height (PDH). After 2 years postoperatively, all patients underwent computed tomography (CT) to evaluate the fusion condition. Perioperative data and related complications were recorded. RESULTS: Sixty-two patients were included (mean age: 82.22 ± 1.95 years). The ISDF and the PLIF groups had 34 and 28 patients, respectively. The average follow-up time was 2.1 ± 0.25 years. There was no significant difference in VAS, ODI, JOA, and PDH scores between both groups preoperatively and at each postoperative time-point. The IA and LL showed significant differences between both groups after surgery (p < 0.05). The postoperative IA in the ISDF group were significantly lower than the preoperative values, while that in the PLIF group were markedly increased. The PLIF group had an increased LL compared with that preoperatively (p < 0.05), while the LL in the ISDF did not significantly change. The operative time, blood loss, hospital stay time, and the rate of perioperative complications of the ISDF group were significantly lower than those of the PLIF group (p < 0.05). There was no significant difference in the fusion rates between both groups. CONCLUSION: ISDF surgery is a viable method for octogenarian patients with LSS that provides a similar clinical efficacy, shorter operative time, less blood loss, shorter hospital stay time, and fewer complications, compared to the PLIF surgery.


Assuntos
Vértebras Lombares/cirurgia , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Fusão Vertebral/métodos , Estenose Espinal/cirurgia , Idoso de 80 Anos ou mais , Constrição Patológica/congênito , Feminino , Humanos , Vértebras Lombares/anormalidades , Vértebras Lombares/diagnóstico por imagem , Masculino , Estudos Retrospectivos , Fusão Vertebral/efeitos adversos , Estenose Espinal/diagnóstico por imagem , Resultado do Tratamento
11.
Neurologist ; 2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35184121

RESUMO

BACKGROUND: Electroencephalogram (EEG) and cerebrospinal fluid (CSF) are widely used in the clinical diagnosis of viral encephalitis (VE), but their value in the diagnosis of VE and the detection rate of abnormal indicators need to be further supported by more clinical data. METHODS: In this study, routine laboratory testing, biochemical examinations of cerebrospinal fluid (CSF) and EEG characteristics were performed in patients with VE to guide the diagnosis and treatment of VE in clinical settings. A total of 330 patients with VE were enrolled in the Department of Neurology of the Third Affiliated Hospital of Zunyi Medical University from January 1, 2015 to January 30, 2020. EEG, routine testing and assessment of biochemical indicators of CSF were performed within 10 days after admission, and the results were analyzed by paired χ2 test to compare the diagnostic value of EEG and CSF for VE. RESULTS: In 330 cases of VE, 283 cases (85.76%) had abnormal EEG, and 189 cases (57.27%) had abnormal CSF indicators. The incidence of EEG abnormalities was higher than that of CSF indicators, and the difference was statistically significant (P<0.05). CONCLUSIONS: Both the EEG and CSF analysis are valuable indicators in the diagnosis of VE patients. Compared with the CSF examination, the EEG examination had a better diagnostic efficacy for the diagnosis of VE. In addition, a normal EEG or a normal CSF level cannot exclude VE, and it is still necessary to develop new diagnostic indicators to cover all viral encephalitides.

12.
Int Urogynecol J ; 2022 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-35083500

RESUMO

INTRODUCTION AND HYPOTHESIS: We aimed to develop a deep learning-based multi-label classification model to simultaneously diagnose three types of pelvic organ prolapse using stress magnetic resonance imaging (MRI). METHODS: Our dataset consisted of 213 midsagittal labeled MR images at maximum Valsalva. For each MR image, the two endpoints of the sacrococcygeal inferior-pubic point line were auto-localized. Based on this line, a region of interest was automatically selected as input to a modified deep learning model, ResNet-50, for diagnosis. An unlabeled MRI dataset, a public dataset, and a synthetic dataset were used along with the labeled image dataset to train the model through a novel training strategy. We conducted a fivefold cross-validation and evaluated the classification results using precision, recall, F1 score, and area under the curve (AUC). RESULTS: The average precision, recall, F1 score, and AUC of our proposed multi-label classification model for the three types of prolapse were 0.84, 0.72, 0.77, and 0.91 respectively, which were improved from 0.64, 0.53, 0.57, and 0.83 from the original ResNet-50. Classification took 0.18 s to diagnose one patient. CONCLUSIONS: The proposed deep learning-based model were demonstrated feasible and fast in simultaneously diagnosing three types of prolapse based on pelvic floor stress MRI, which could facilitate computer-aided prolapse diagnosis and treatment planning.

13.
J Virol ; 96(1): e0149221, 2022 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-34668773

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in more than 235 million cases worldwide and 4.8 million deaths (October 2021), with various incidences and mortalities among regions/ethnicities. The coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63 utilize the angiotensin-converting enzyme 2 (ACE2) as the receptor to enter cells. We hypothesized that the genetic variability in ACE2 may contribute to the variable clinical outcomes of COVID-19. To test this hypothesis, we first conducted an in silico investigation of single-nucleotide polymorphisms (SNPs) in the coding region of ACE2. We then applied an integrated approach of genetics, biochemistry, and virology to explore the capacity of select ACE2 variants to bind coronavirus spike proteins and mediate viral entry. We identified the ACE2 D355N variant that restricts the spike protein-ACE2 interaction and consequently limits infection both in vitro and in vivo. In conclusion, ACE2 polymorphisms could modulate susceptibility to SARS-CoV-2, which may lead to variable disease severity. IMPORTANCE There is considerable variation in disease severity among patients infected with SARS-CoV-2, the virus that causes COVID-19. Human genetic variation can affect disease outcome, and the coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63 utilize human ACE2 as the receptor to enter cells. We found that several missense ACE2 single-nucleotide variants (SNVs) that showed significantly altered binding with the spike proteins of SARS-CoV, SARS-CoV-2, and NL63-HCoV. We identified an ACE2 SNP, D355N, that restricts the spike protein-ACE2 interaction and consequently has the potential to protect individuals against SARS-CoV-2 infection. Our study highlights that ACE2 polymorphisms could impact human susceptibility to SARS-CoV-2, which may contribute to ethnic and geographical differences in SARS-CoV-2 spread and pathogenicity.


Assuntos
Enzima de Conversão de Angiotensina 2/genética , COVID-19/genética , Predisposição Genética para Doença/genética , Enzima de Conversão de Angiotensina 2/metabolismo , Variação Genética , Humanos , Polimorfismo de Nucleotídeo Único , Ligação Proteica , SARS-CoV-2/metabolismo , SARS-CoV-2/patogenicidade , Glicoproteína da Espícula de Coronavírus/metabolismo , Internalização do Vírus
14.
Int J Cardiovasc Imaging ; 38(1): 15-23, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34783930

RESUMO

Left ventricular diastolic dysfunction (LVDD) can be affected by many factors, including epicardial adipose tissue (EAT), obesity and type-2 diabetes mellitus (T2DM). The aim of this study was to establish and validate an easy-to-use nomogram that predicts the severity of LVDD in patients with T2DM. This is a retrospective study of 84 consecutive subjects with T2DM admitted to the Endocrinology Department, the First People's Hospital of Zunyi City between January 2015 and October 2020. Several echocardiographic characteristics were used to diagnose diastolic dysfunction according to the 2016 diastolic dysfunction ASE guidelines. Anthropometric, demographic, and biochemical parameters were collected. Through a least absolute shrinkage and selection operator (LASSO) regression model, we reduced the dimensionality of the data and determined factors for the nomogram. The mean follow-up was 25.97 months. Cases were divided into two groups, those with LVDD (31) and those without (53). LASSO regression identified total cholesterol (Tol.chol), low-density lipoprotein (LDL), right ventricular anterior wall (RVAW) and epicardial adipose tissue (EAT) were identified as predictive factors in the nomogram. The ROC curve analysis demonstrated that the AUC value for most clinical paramerters was higher than 0.6. The nomogram can be used to promote the individualized prediction of LVDD risk in T2DM patients, and help to prioritize patients diagnosed with echocardiography.


Assuntos
Diabetes Mellitus Tipo 2 , Disfunção Ventricular Esquerda , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/diagnóstico , Humanos , Nomogramas , Valor Preditivo dos Testes , Estudos Retrospectivos , Fatores de Risco , Disfunção Ventricular Esquerda/diagnóstico por imagem , Disfunção Ventricular Esquerda/etiologia , Função Ventricular Esquerda
15.
Minim Invasive Ther Allied Technol ; 31(4): 525-530, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33433250

RESUMO

PURPOSE: To evaluate the efficacy of stent-in-stent (SIS) and side-by-side (SBS) bilateral stenting for treating malignant hilar biliary obstruction (MHBO). MATERIAL AND METHODS: Relevant studies in Pubmed, Embase, and Cochrane Library were identified through June 2020. This meta-analysis was conducted using RevMan v5.3, using relevant endpoint data relating to clinical and technical success, complications, stent dysfunction, and overall survival (OS) rates extracted from these studies. RESULTS: We identified six relevant studies which included 315 MHBO patients treated with either SBS (n = 161) or SIS bilateral (n = 154) stenting. We saw no significant difference between these two groups with respect to clinical success (OR: 1.07; 95% CI: 0.46, 2.49, p = .87), complication (HR: 0.12; 95% CI: -0.04, 0.27, p = .15), stent dysfunction (OR: 0.68; 95% CI: 0.42, 1.10, p = .11), or OS (HR: 0.97; 95% CI: 0.82, 1.16, p = .74). However, the SBS group exhibited significantly lower technical success rates (OR: 6.55; 95% CI: 1.10, 38.83, p = .04). Significant heterogeneity was only detected for the endpoint of complication rates (I2 = 60%). CONCLUSION: These results suggest that SIS bilateral stenting yields better rates of technical success than does SBS bilateral stenting in MHBO patients.


Assuntos
Neoplasias dos Ductos Biliares , Colestase , Neoplasias dos Ductos Biliares/cirurgia , Colestase/complicações , Colestase/cirurgia , Humanos , Estudos Retrospectivos , Stents/efeitos adversos , Taxa de Sobrevida , Resultado do Tratamento
16.
Med Phys ; 49(2): 1083-1096, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34967014

RESUMO

PURPOSE: High-resolution pelvic magnetic resonance (MR) imaging is important for the high-resolution and high-precision evaluation of pelvic floor disorders (PFDs), but the data acquisition time is long. Because high-resolution three-dimensional (3D) MR data of the pelvic floor are difficult to obtain, MR images are usually obtained in three orthogonal planes: axial, sagittal, and coronal. The in-plane resolution of the MR data in each plane is high, but the through-plane resolution is low. Thus, we aimed to achieve 3D super-resolution using a convolutional neural network (CNN) approach to capture the intrinsic similarity of low-resolution 3D MR data from three orientations. METHODS: We used a two-dimensional (2D) super-resolution CNN model to solve the 3D super-resolution problem. The residual-in-residual dense block network (RRDBNet) was used as our CNN backbone. For a given set of low through-plane resolution pelvic floor MR data in the axial or coronal or sagittal scan plane, we applied the RRDBNet sequentially to perform super-resolution on its two projected low-resolution views. Three datasets were used in the experiments, including two private datasets and one public dataset. In the first dataset (dataset 1), MR data acquired from 34 subjects in three planes were used to train our super-resolution model, and low-resolution MR data from nine subjects were used for testing. The second dataset (dataset 2) included a sequence of relatively high-resolution MR data acquired in the coronal plane. The public MR dataset (dataset 3) was used to demonstrate the generalization ability of our model. To show the effectiveness of RRDBNet, we used datasets 1 and 2 to compare RRDBNet with interpolation and enhanced deep super-resolution (EDSR) methods in terms of peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) index. As 3D MR data from one view have two projected low-resolution views, different super-resolution orders were compared in terms of PSNR and SSIM. Finally, to demonstrate the impact of super-resolution on the image analysis task, we used datasets 2 and 3 to compare the performance of our method with interpolation on the 3D geometric model reconstruction of the urinary bladder. RESULTS: A CNN-based method was used to learn the intrinsic similarity among MR acquisitions from different scan planes. Through-plane super-resolution for pelvic MR images was achieved without using high-resolution 3D data, which is useful for the analysis of PFDs.


Assuntos
Imageamento por Ressonância Magnética , Diafragma da Pelve , Humanos , Processamento de Imagem Assistida por Computador , Redes Neurais de Computação , Diafragma da Pelve/diagnóstico por imagem , Razão Sinal-Ruído
17.
Colloids Surf B Biointerfaces ; 209(Pt 2): 112188, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34742021

RESUMO

Some microbial strains are ideal producers of extracellular enzymes that can be used in various industries. However, in many fields, especially in the pharmaceutical field, these enzymes need to be recovered and purified through multistep processes and tedious procedures before they can be used. The recovery process is difficult and increases the cost of enzyme production. Therefore, reducing purification steps will greatly benefit the utilization of microbial enzymes. The 35 M strain of Bacillus amyloliquefaciens, which has high extracellular protease production, was isolated from a phosphate mine. When cultured in a medium with soybean meal as the main component, the maximum activity of extracellular protease reached 16,992 U/mL. SDS-PAGE showed that there were two main proteins in the fermentation supernatant, with a paucity of other defined protein bands. Mass spectrometry and zymogram analysis showed that the two main bands were two proteases, corresponding to alkaline protease (AprM) and neutral protease (NprM), respectively. Gene cloning, sequencing, and further comparisons were used to confirm AprM and NprM correspond to these proteases from B. amyloliquefaciens. Notably, SDS-PAGE and zymogram analysis showed that NprM had obviously higher catalytic efficiency toward casein than did AprM. Strain 35 M is a promising protease producer with great potential for applications in industrial protease production. Additionally, this study demonstrates strain 35 M may be particularly well suited to use in degrading anti-nutritional factors in soybean meal, so as to improve the nutritional value of soybean meal.


Assuntos
Bacillus amyloliquefaciens , Bacillus amyloliquefaciens/genética , Bacillus amyloliquefaciens/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Clonagem Molecular , Fermentação , Peptídeo Hidrolases/metabolismo , Soja
18.
Bioact Mater ; 10: 345-354, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34901551

RESUMO

Spinal acrylic bone cements (ABCs) are used clinically for percutaneous vertebroplasty (PVP) and kyphoplasty (PKP) to treat osteoporotic vertebral compression fractures. Product translation of spinal ABC products followed the design control processes including design verification and validation. The bench to bedside translation of the first Chinese spinal ABC product (Alliment®, namely Alliment Cement) approved by National Medical Products Administration of China was investigated and another commercial product served as the control (Osteopal®V, namely Osteopal V Cement). Results of non-clinical bench performance verification tests of compression, bending and monomer release showed that the newly marketed Alliment Cement is similar to the Osteopal V Cement with properties of both meeting the criteria specified by standards. The Alliment Cement demonstrated good biocompatibility during the 26 weeks' bone implantation test. Porcine cadaver validation tests further revealed that the Alliment Cement satisfied the needs for both PVP and PKP procedures. A post-approval, retrospective clinical investigation further demonstrated the safety and efficacy of the Alliment Cement, with a significant reduction of pain and the improved stability of the fractured vertebral bodies. A successful translation of biomaterial medical products needs close collaborations among academia, industry, healthcare professionals and regulatory agencies.

19.
J Chromatogr A ; 1662: 462725, 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-34922282

RESUMO

Semi-packed columns are microfabricated gas chromatography columns which have a large surface area and high aspect ratio. In this paper, a new semi-packed column with high-density elliptic cylindrical posts (SCHECP) made by a micro-electro-mechanical system (MEMS) technique was reported. Compared to a semi-packed column with cylindrical posts (SCCP) under the same effective width, the surface area and aspect ratio of SCHECP were improved by 71.19% and 76.47%, respectively. To compare the performance of these two semi-packed columns, SCHECP and SCCP were fabricated. A 10-nm thick alumina film was deposited as the stationary phase by atomic layer deposition technique to ensure the uniformity and repeatability of the stationary-phase film. A contrast experiment was conducted, and the results showed that compared with SCCP, better separation performance was realized in SCHECP due to the increase in surface area and aspect ratio. The number of theoretical plates of nonane was increased by 541.84%, and the tailing factor was decreased by 54.31%.


Assuntos
Sistemas Microeletromecânicos , Óxido de Alumínio , Cromatografia Gasosa
20.
Zhonghua Nan Ke Xue ; 27(9): 833-839, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34914261

RESUMO

Sexual arousal is an important factor for the success of sexual behavior, and regulated by the central nervous system, its underlying mechanism is very complicated. Androgen is the most important endocrine hormone in men, which is deeply involved in the whole process of male sexual response, but how it regulates male sexual arousal has not been fully clarified and remains one of the hotspots in current andrological research. Therefore, this paper presents an overview of the advances in the studies of the related role and mechanism of androgen in male sexual arousal. In the central nervous system, androgen regulates the release of dopamine neurotransmitters by binding androgen receptors or metabolizing neurosteroids, thus activating the brain reward system. Besides, androgen regulates the neuronal plasticity and spinous process formation in the neural circuit of sexual arousal to ensure successful activation and conduction of the neural circuit. However, the specific regulating mechanism of sexual arousal remains to be further explored.


Assuntos
Androgênios , Excitação Sexual , Humanos , Masculino
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