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1.
ACS Nano ; 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36722955

RESUMO

Single atom catalysts (SACs) with isolated active sites exhibit the highest reported mass activity for hydrogen evolution catalysis, which is crucial for practical applications. Here, we demonstrate that ultrahigh mass activity can also be achieved by rationally merging the isolated platinum (Pt) active sites in SAC. The catalyst was obtained by the thermodynamically driven diffusing and merging phosphorus-doped carbon (PC) supported Pt single atoms (Pt1@PC) into Pt nanoclusters (PtM@PC). X-ray absorption spectroscopy analysis revealed that the merged nanoclusters exhibit much stronger interactions with the support than the traditional method, enabling more efficient electron transfer. The optimized PtM@PC exhibited an order of magnitude higher mass activity (12.7 A mgPt-1) than Pt1@PC (0.9 A mgPt-1) at an overpotential of 10 mV in acidic media, which is the highest record to date, far exceeding reports for other outstanding SACs. Theoretical study revealed that the collective active sites in PtM@PC exhibit both favorable hydrogen binding energy and fast reaction kinetics, leading to the significantly enhanced mass activity. Despite its low Pt content (2.2 wt %), a low hydrogen production cost of ∼3 USD kg-1 was finally achieved in the full-water splitting at a laboratory scale.

2.
Neurochem Res ; 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36723726

RESUMO

Lignans are the main components of Syringa pinnatifolia Hemsl. (SP). Previous studies have shown that SP lignans (SPL) can considerably improve CCl4-induced acute liver injury in mice by the anti-oxidative stress (OS) mechanism. In this study, we investigated the antioxidant effects of SPL on cerebral ischemia/reperfusion injury (CIRI) and its underlying molecular mechanism. We developed a middle cerebral artery occlusion/reperfusion (MCAO/R) model in mice to achieve CIRI and orally administered SPL daily for 1-3 days. We evaluated neurological function deficits and performed hematoxylin and eosin staining. We further calculated the infarct volume. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the brain were detected using corresponding kits. The transcription and protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) in brain tissues were analyzed by real-time reverse transcription polymerase chain reaction and western blotting, respectively. The results showed that SPL could remarkably ameliorate neurological functions and pathological damage in brain tissues, reducing the cerebral infarct volume. It also increased the SOD and GPx activities decreased the MDA levels as well as inhibited the expression of (NOX)2 and NOX4. We also found that the mRNA and protein levels of Nrf2, HO-1, and NQO1 in the CIRI mice increased transiently and peaked at 24 h of reperfusion, and then began to decline. SPL could reverse decreasing Nrf2 and HO-1 levels after 24 h. In conclusion, SPL can alleviate CIRI and OS by activating the Nrf2/HO-1 pathway.

3.
Anal Chem ; 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36706440

RESUMO

Breast cancer is the most commonly diagnosed cancer type worldwide. Overexpression of human epidermal growth factor receptor 2 (HER2) is an important subtype of breast cancer and results in an increased risk of recurrence and metastasis in patients. At present, immunohistochemistry (IHC) is used to detect the expression of HER2 in breast cancer tissues as the golden standard. However, IHC has some shortcomings, such as large subjective impact, long time consumption, expensive reagents, etc. In this paper, a combined morphological and spectroscopic diagnostic method based on label-free surface-enhanced Raman scattering (SERS) for HER2 expression in breast cancer is proposed. It can not only quantitively detect HER2 expression in breast cancer tissues by spectroscopic measurements but also give morphological images reflecting the distribution of HER2 in tissues. The results show that the consistency between this method and IHC is 95% and achieves the annotation of tumor regions on tissue sections. This method is time-consuming, quantifiable, intuitive, scalable, and easy to understand. Combined with deep learning approaches, it is expected to promote the development of clinical detection and diagnosis technology for breast cancer and other cancers.

4.
J Mol Model ; 29(2): 58, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36715793

RESUMO

CONTEXT: With the continuous improvement of anticancer drugs, the condition of patients has been controlled to a certain extent, but the problem that still needs to be urgently solved is that most anticancer drug candidates' solubility is low. On the one hand, the low solubility of anticancer drugs may lead to a decrease in the absorption rate of anticancer drugs, poor treatment effect, and even death in severe cases. On the other hand, it will also lead to a waste of medical resources. At the same time, the rapid and scientific screening of ideal anticancer drugs has become a difficult problem that researchers have to face in the research process. In this study, we found two kinds of SN38-ligand complexes that solubilize 7-ethyl-10-hydroxycamptothecin (SN38) through molecular docking and molecular dynamics simulation methods. This process not only provided valuable information on improving the solubility of SN38, but also helped to discover effective potential complexes that solubilize SN38 quickly and scientifically. METHODS: The interaction of the SN38 with folic acid and isoproterenol hydrochloride was rapidly determined by molecular docking and molecular dynamics simulation methods. We used Discovery Studio software to perform molecular docking. And then, we used Gromacs 2019.3 software to perform molecular dynamics, analyzing and comparing the hydrogen bonds, solvent-accessible surface areas, energies, and so on between SN38 and SN38-ligand complexes. And the force field adopted the Gromos 54a7.


Assuntos
Antineoplásicos , Camptotecina , Humanos , Irinotecano/química , Camptotecina/farmacologia , Camptotecina/química , Simulação de Dinâmica Molecular , Simulação de Acoplamento Molecular , Ligantes , Antineoplásicos/química
6.
Cardiooncology ; 9(1): 1, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36604733

RESUMO

BACKGROUND: There is limited data on the impact of cardiac disease on long term outcomes of allogeneic stem cell transplant (alloSCT). Our study aims to describe the incidence of late cardiac events after alloSCT, identify risk factors for developing a late cardiac event, and illustrate the impact of late cardiac events on overall survival. METHODS: Patients who underwent alloSCT from 2007 to 2017 and survived more than 1 year after transplant (N = 804) were included. Gray's sub-distribution methods, while accounting for death as a competing risk, were used to calculate the cumulative incidence of late cardiac events. Univariate regression models based on Gray's sub-distribution were fitted to assess the potential predictive effects of baseline characteristics on the risk of developing any late cardiac events. Univariate Cox proportional hazard regression models were used to evaluate the association between late cardiac events and overall survival. RESULTS: The cumulative incidence of a late cardiac event at 5 years after transplant was 22% (95% CI 19-25%). The most frequent cardiac event was a decline in LVEF to < 45% with a cumulative incidence of 9% (95% CI 7-11%). Patients were at significantly increased hazard of developing a late cardiac event if they had a history of congestive heart failure prior to alloSCT (HR 4.53, 95% CI 2.57-7.97, p-value < 0.001), a decline in LVEF to < 45% (HR 3.95, 95% CI 2.09-7.47, p-value < 0.001) or cerebral vascular accident (HR 3.13, 95% CI 1.38-7.06, p-value 0.004). Transplant characteristics such as primary disease, donor type, use of TBI, myeloablative conditioning regimen or tyrosine kinase inhibitor had no significant association with late cardiac events. Almost all cardiac events demonstrated a significantly increased risk of death. This hazard was the highest in patients who experienced an atrial arrhythmia (HR 10.6, 95% CI 7.7-14.6). CONCLUSION: Adverse cardiac events are relatively common late after alloSCT with identifiable risk factors such as medical comorbidities prior to transplant and are associated with a negative impact on overall survival.

7.
J Opt Soc Am A Opt Image Sci Vis ; 40(1): 10-20, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36607070

RESUMO

Diffuse optical tomography (DOT) is a non-invasive imaging modality that uses near-infrared light to probe the optical properties of tissue. In conventionally used deterministic methods for DOT inversion, the measurement errors were not taken into account, resulting in unsatisfactory noise robustness and, consequently, affecting the DOT image reconstruction quality. In order to overcome this defect, an extended Kalman filter (EKF)-based DOT reconstruction algorithm was introduced first, which improved the reconstruction results by incorporating a priori information and measurement errors to the model. Further, to mitigate the instability caused by the ill-condition of the observation matrix in the tomographic imaging problem, a new, to the best of our knowledge, estimation algorithm was derived by incorporating Tikhonov regularization to the EKF method. To verify the effectiveness of the EKF algorithm and Tikhonov regularization-based EKF algorithm for DOT imaging, a series of numerical simulations and phantom experiments were conducted, and the experimental results were quantitatively evaluated and compared with two conventionally used deterministic methods involving the algebraic reconstruction technique and Levenberg-Marquardt algorithm. The results show that the two EKF-based algorithms can accurately estimate the location and size of the target, and the imaging accuracy and noise robustness are obviously improved. Furthermore, the Tikhonov regularization-based EKF obtained optimal parameter estimations, especially under the circumstance of low absorption contrast (1.2) and high noise level (10%).


Assuntos
Processamento de Imagem Assistida por Computador , Tomografia Óptica , Processamento de Imagem Assistida por Computador/métodos , Tomografia Óptica/métodos , Algoritmos
8.
Artigo em Inglês | MEDLINE | ID: mdl-36622416

RESUMO

BACKGROUND: Lateral lymph node metastasis (LLNM) is associated with poor prognosis in patients with papillary thyroid cancer (PTC). The purpose of this study was to determine the risk factors for LLNM and establish prediction models that could individually assessed the risk of LLNM. METHODS: A total of 619 PTC patients were retrospectively analyzed in our study. Univariate and multivariate analysis were performed for male and female patients, respectively, to assess relationships between clinicopathological features and LLNM. By integrating independent predictors selected by binary logistic regression modeling, preoperative and postoperative nomograms were developed to estimate the risk of LLNM. RESULTS: LLNM was detected in 80 of 216 male patients. Of 403 female patients, 114 had LLNM. The preoperative nomogram of male patients included three clinical variables: the number of foci, tuner size, and echogenic foci. In addition to the above three variables, the postoperative nomogram of male patients included extrathyroidal extension (ETE) detected in surgery, central lymph node metastasis (CLNM) and high-volume CLNM. The preoperative nomogram of female patients included the following variables: age, chronic lymphocytic thyroiditis (CLT), BRAF V600E, the number of foci, tumor size and echogenic foci. Variables such as CLT, BRAF V600E, the number of foci, tumor size, ETE detected in surgery, CLNM, high-volume CLNM and central lymph node ratio were included in the postoperative nomogram. Above Nomograms show good discrimination. CONCLUSIONS: Considering the difference in the incidence rate of LLNM between men and women, a separate prediction system should be established for patients of different genders. These nomograms are helpful in promoting the risk stratification of PTC treatment decision-making and postoperative management.

9.
Small ; : e2207525, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36627258

RESUMO

Defects, such as unsaturated coordination centers and vacancies, can fundamentally change materials' inherent properties and growth habits. The development of defect engineering has promoted the application of many technologies, but it is still a great challenge to selectively manufacture defect sites in existing material systems. It is shown here that in situ site-directed tailoring of metal sites in Prussian blue analogs (PBA) can be achieved according to the reducibility differences of different metal atoms, forming naturally nonpreferred unsaturated coordination centers. Meanwhile, the in situ capture of small reducing molecule can realize site-directed tailoring of crystal facets during crystal growth and results in oriented 1D growth. As an oxygen evolution reaction catalyst, the resulted PBA with the nonpreferred unsaturated coordination centers shows a low overpotential of 239 mV at 10 mA cm-2 in alkali, superior to the original PBAs and the previously reported defective PBA derivatives, which can be ascribed to the unsaturated coordination active center and the unique 1D structure. This work opens up opportunities for producing naturally nonpreferred unsaturated coordination center in nanomaterials for broad applications.

10.
Cancer Med ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36629124

RESUMO

BACKGROUND: Debates exist on the treatment decision of the stage II/III colorectal cancer (CRC) due to the insufficiency of the current TNM stage-based risk stratification system. Epithelial-mesenchymal transition (EMT) and tumor microenvironment (TME) have both been linked to CRC progression in recent studies. We propose to improve the prognosis prediction of CRC by integrating TME and EMT. METHODS: In total, 2382 CRC patients from seven datasets and one in-house cohort were collected, and 1640 stage II/III CRC patients with complete survival information and gene expression profiles were retained and divided into a training cohort and three independent validation cohorts. Integrated analysis of 398 immune, stroma, and epithelial-mesenchymal transition (ISE)-related genes identified an ISE signature independently associated with the recurrence of CRC. The underlying biological mechanism of the ISE signature and its influence on adjuvant chemotherapy was further explored. RESULTS: We constructed a 26-gene signature which was significantly associated with poor outcome in Training cohort (p < 0.001, HR [95%CI] = 4.42 [3.25-6.01]) and three independent validation cohorts (Validation cohort-1: p < 0.01, HR [95%CI] = 1.70 [1.15-2.51]; Validation cohort-2: p < 0.001, HR [95% CI] = 2.30 [1.67-3.16]; Validation cohort-3: p < 0.01, HR [95% CI] = 2.42 [1.25-4.70]). After adjusting for known clinicopathological factors, multivariate cox analysis confirmed the ISE signature's independent prognostic value. Subgroup analysis found that stage III patients with low ISE score might benefit from adjuvant chemotherapy (p < 0.001, HR [95%CI] = 0.15 [0.04-0.55]). Hypergeometric test and enrichment analysis revealed that low-risk group was enriched in thr immune pathway while high-risk group was associated with the EMT pathway and CMS4 subtype. CONCLUSION: We proposed an ISE signature for robustly predicting the recurrence of stage II/III CRC and help treatment decision by identifying patients who will not benefit from current standard treatment.

11.
Artigo em Chinês | MEDLINE | ID: mdl-36597368

RESUMO

Objective:The purpose of this study was to investigate the clinical characteristics and risk factors of adult recurrent acute infectious epiglottitis. Methods:All patients diagnosed with acute infectious epiglottitis hospitalized in the Department of Otolaryngology, Hai'an People's Hospital, Nantong University from January 2012 to December 2019 were included. Results:The recurrence rate of 331 adult patients with acute infectious epiglottitis was 4.2% (14/331), including 10 cases of once recurrence and 4 cases of twice recurrence. The onset time of all patients was within 48 hours. The most common main complaint in the recurrent group was sore throat (42.9%), and dysphagia in the non-recurrent group (42.0%). The frequency of drinking in recurrent group was higher than that in non-recurrent group (P=0.009). The incidence of chronic obstructive pulmoriary disease(COPD), diabetes, cyst and gastroesophageal reflux disease/laryngopharyngeal reflux disease in recurrent group was higher than that in non-recurrent group. There was no significant difference in other clinical features, treatment and prognosis between the two groups except tongue tonsil infection under laryngoscope. Multivariate analysis showed that frequent drinking (more than twice a week), COPD, diabetes, cysts and lingual tonsillar infection were the risk factors for recurrence. Conclusion:Adult acute infectious epiglottitis has a proportion of single or multiple recurrence. Frequent drinking, COPD, diabetes, cyst and lingual tonsillar infection are the risk factors for the recurrence.


Assuntos
Cistos , Epiglotite , Refluxo Laringofaríngeo , Doença Pulmonar Obstrutiva Crônica , Humanos , Adulto , Epiglotite/diagnóstico , Epiglotite/terapia , Fatores de Risco , Doença Aguda
12.
J Vet Diagn Invest ; : 10406387221147319, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36600502

RESUMO

Rhabdomyosarcoma (RMS), a malignant mesenchymal neoplasm derived from skeletal muscle, is relatively rare in both human and veterinary medicine. Here we report an unusual case of invasive spindle-cell RMS (SCRMS) with bone infiltration and pathologic fracture in a 3.5-y-old intact female Bulldog. Radiographically, a large, predominantly osteolytic mass in the tibia and fibula of the left hindlimb had features typical of a malignant primary bone tumor. Clinically, osteosarcoma was suspected, and the leg was amputated. Histologically, the mass was composed of loosely interwoven spindle-cell fascicles; tumor cells were fusiform with cigar-shaped nuclei and abundant eosinophilic cytoplasm. The neoplastic cells were strongly immunopositive for vimentin, muscle-specific actin, desmin, myogenin, and myoD1. Invasive SCRMS with osteolysis was diagnosed based on the histologic examination and immunohistochemical (IHC) stains. The dog was alive without any evidence of local recurrence or distant metastasis 18 mo post-surgery. RMS should be included in the differential diagnosis when osteolysis occurs; IHC staining confirmation is of great value for definitive diagnosis and treatment planning.

13.
Dent Mater ; 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36641338

RESUMO

Gelatin-methacryloyl hydrogels (GelMA) have demonstrated their utility as scaffolds in a variety of tissue engineering applications. OBJECTIVES: In this study, a highly functionalized GelMA hydrogel was synthesized and assessed for degree of functionalization. As the proposed GelMA hydrogel was coupled to a visible-light photoinitiator, we hypothesized it might serve as base to formulate a model dentin primer for application in restorative dentistry. METHODS: GelMA was mixed with photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP), photopolymerized for 0-40 s using a dental light-curing device and tested for extrudability, degree of photo-crosslinking (DPxlink), water sorption/solubility/swelling (WS/SL/SW) and apparent modulus of elasticity (AE). Model dentin primer was prepared by mixing GelMA+LAP with a primer of a commercial three-step etch-and-rinse adhesive. After application of GelMA-based primer to acid-etched dentin, samples were bonded with correspondent adhesive agent, photopolymerized and had their immediate bond strength compared to control samples primed and bonded with the same commercial material. RESULTS: Extrudability of hydrogel was confirmed using a microsyringe to write the acronym "CDMI". DPxlink of GelMA+LAP changed significantly as a function of photopolymerization time (20 s < 30 s ≤ 40 s). WS, SL and SW were significantly reduced in hydrogels polymerized for 30 and 40 s. AE of hydrogels varied significantly as a function of photopolymerization time (20 s < 30 s ≤ 40 s; 20 s ‡ 40 s). Bond strength of dentin primed with GelMA-based primer was lower (∼29.3 MPa) but not significantly of that of control (∼34.6 MPa). CONCLUSIONS: Optimization of a GelMA-based dentin primers can lead to the development of promising biomimetic adhesives for dentin rehabilitation.

14.
Chemosphere ; 316: 137880, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36649892

RESUMO

This study explored the feasibility of a coupled system for antibiotic removal and biofuel production through microalgae cultivation. Initial, batch culture experiments demonstrated that sulfadiazine (SDZ) had an inhibitory effect on Chlorella sp. G-9, and 100.0 mg L-1 SDZ completely inhibited its growth. In order to improve SDZ removal efficiency by microalgae, three membrane photobioreactors (MPBRs) with different hydraulic retention times (HRTs) were established for continuous microalgae cultivation. The efficient coupling of SDZ removal and microalgal lipid production was achieved through the gradual increment of influent SDZ concentration from 0 to 100.0 mg L-1. The reduction in SDZ ranged between 57.8 and 89.7%, 54.7-91.7%, and 54.6-93.5% for the MPBRs with HRT of 4 d, 2 d, and 1 d, respectively. Chlorella sp. Was found to tolerate higher concentrations of SDZ in the MPBR system, and the resulting stress from high concentrations of SDZ effectively increased the lipid content of microalgae for potential biodiesel production. With the increase of influent SDZ concentration from 0 to 100.0 mg L-1, the lipid content of microalgae increased by 43.5%. Chlorophyll content, superoxide dismutase activity, and malondialdehyde content of microalgae were also evaluated to explore the mechanism of microalgae tolerance to SDZ stress in MPBR.


Assuntos
Chlorella , Microalgas , Sulfadiazina , Fotobiorreatores , Biomassa , Biocombustíveis , Lipídeos
15.
Biochim Biophys Acta Mol Basis Dis ; 1869(4): 166638, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36657499

RESUMO

OBJECTIVE: Atrial fibrillation (AF) is the most common tachyarrhythmia in urgent need of therapeutic optimization. Obesity engenders AF, and its pathogenesis is closely intertwined with insulin resistance (IR), but mechanism-based management is still underinvestigated. Intermittent fasting (IF) is a novel lifestyle intervention that mitigates IR, a potential AF driver, yet whether IF can prevent obesity-related AF remains elusive. Here, we aimed to evaluate the impacts of short-term IF on AF and to uncover the underlying mechanism. METHODS: We subjected obese mice (high-fat diet for 8-week) to IF (alternative-day fasting for another 5-week) for AF vulnerability and substrate formation assessment, and similarly treated neonatal atrial cardiomyocytes (NRCMs) and fibroblasts (NRCFs) (palmitate, 200 µM) with IF (alternative-day short-term starvation for 8-day) for mechanism investigation. RESULTS: Obese mice were prone to AF and atrial remodeling. IF reduced AF inducibility, duration, and reversed atrial remodeling including channel disturbance, left atrial dilation, cardiac hypertrophy and fibrosis in obese mice independent of weight loss. Mechanistically, IF up-regulated the SIRT3 protein level both in vivo and in vitro, and pharmacologic inhibition (3-(1H-1,2,3-Triazol-4-yl) pyridine, 50 µM) and genetic suppression of SIRT3 could attenuate the IF-mediated benefits against hypertrophy and fibrosis. Furthermore, IF activated AMPK and Akt signaling, two positive downstream targets of SIRT3, and inactivated HIF1α signaling, a negative downstream target of SIRT3 in both obese mice atria and palmitate-treated cells, while inhibition of SIRT3 reversed these effects. CONCLUSION: IF prevents obesity-related AF via SIRT3-mediated IR mitigation, thus representing a feasible lifestyle intervention to improve AF management.

16.
Biochim Biophys Acta Mol Basis Dis ; 1869(3): 166637, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36638874

RESUMO

The demand of exploring strategies to enhance chemotherapy drug efficacy and alleviate adverse effects by using natural compounds is increasing. Sinensetin (SIN) is a kind of natural flavonoids with anti-inflammatory activities. However, its protective impact on chemotherapy-induced adverse effects has not been well demonstrated. Here, we found that SIN could inhibit Cisplatin-induced release of proinflammatory cellular contents and inflammatory cell death-pyroptosis. In addition, Cisplatin-induced activation of gasdermin E (GSDME), a critical mediator of chemotherapy-induced tissue injury, could also be reversed by SIN. Furthermore, SIN impaired Cisplatin-induced intracellular damages, including ROS release and DNA damages. Importantly, SIN was able to alleviate intestinal injury in Cisplatin-challenged mice, which was accompanied by the decrease of lytic cell death and immune cell infiltration. Of note, SIN administration did not reverse Cisplatin-caused tumor suppression in vivo. In conclusion, our result provides a potential application of SIN to reduce Cisplatin-caused adverse effects, without impairing its anti-tumor capacity.


Assuntos
Antineoplásicos , Cisplatino , Camundongos , Animais , Cisplatino/efeitos adversos , Piroptose , Flavonoides/farmacologia , Flavonoides/uso terapêutico , Antineoplásicos/farmacologia
17.
Artigo em Inglês | MEDLINE | ID: mdl-36640826
18.
Nanomaterials (Basel) ; 13(2)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36678032

RESUMO

Herein, three types of graphene oxides (GOs, GO-M1, GO-M2 and GO-M3) have been successfully prepared from graphite by three different methods and utilized for the removal of Cr(VI) from aqueous solutions. Further, the effects of initial concentration and pH, adsorbent dosage, contact time and temperature on the adsorption performance of GOs were investigated by batch adsorption experiments. Furthermore, the adsorption mechanisms for Cr(VI) adsorption by GOs are mainly the redox reaction and electrostatic attraction, while there are also pore filling, ion exchange and complexation involved in these adsorption processes. The adsorption kinetic and isotherm data indicate that these adsorption processes of GOs on Cr(VI) are dominantly monolayer chemisorption and equilibrium can be reached in 30 min. The saturation adsorption capacities (Qm, 298.15 K) of GO-M1, GO-M2 and GO-M3 for Cr(VI) are estimated to be 3.5412 mg⋅g-1, 2.3631 mg⋅g-1 and 7.0358 mg⋅g-1, respectively. Moreover, the adsorption thermodynamic study showed that these adsorption processes of Cr(VI) by the three types of GOs at 298.15 K to 323.15 K are endothermic, entropy-driven and thermodynamically spontaneous and feasible. Overall, these findings provided vital insights into the mechanism and application of Cr(VI) removal by GOs.

19.
J Biophotonics ; : e202200362, 2023 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-36617540

RESUMO

As a burgeoning medical imaging method based on hybrid fusion of light and ultrasound, photoacoustic imaging (PAI) has demonstrated high potential in various biomedical applications, especially in revealing the functional and molecular information to improve diagnostic accuracy. However, stemming from weak amplitude and unavoidable random noise, caused by limited laser power and severe attenuation in deep tissue imaging, PA signals are usually of low signal-to-noise ratio, and reconstructed PA images are of low quality. Despite that conventional Kalman filter (KF) can remove Gaussian noise in time domain, it lacks adaptability in real-time estimation due to its fixed model. Moreover, KF-based denoising algorithm has not been applied in PAI before. In this paper, we propose an adaptive modified KF (MKF) targeted at PAI denoising by tuning system noise matrix Q and measurement noise matrix R in the conventional KF model. Additionally, in order to compensate the signal skewing caused by MKF, we cascade the backward part of Rauch-Tung-Striebel smoother, which utilizes the newly determined Q. Finally, as a supplement, we add a commonly used differential filter to remove in-band reflection artifacts. Experimental results using phantom and ex vivo colorectal tissue are provided to prove validity of the algorithm.

20.
Int J Mol Sci ; 24(2)2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36674931

RESUMO

Trichosanthin (TCS) is a type I ribosome-inactivating protein extracted from the tuberous root of the plant Trichosanthes. TCS shows promising potential in clinical drug abortion, anti-tumor and immunological regulation. However, the molecular mechanisms of its anti-tumor and immune regulation properties are still not well discovered. In the present study, we investigated the anti-tumor activity of TCS in hepatocellular carcinoma (HCC), both in vitro and in vivo. Both HCC cell lines and xenograft tumor tissues showed considerable growth inhibition after they were treated with TCS. TCS provoked caspase-mediated apoptosis in HCC cells and xenograft tumor tissues. The recruitment of CD8+ T cells to HCC tissues and the expression of chemokines, CCL2 and CCL22, were promoted upon TCS treatment. In addition, TCS induced an upregulation of Granzyme B (GrzB), TNF-α and IFN-γ in HCC tissues, which are the major cytotoxic mediators produced by T cells. Furthermore, TCS also resulted in an increase of mannose-6-phosphate receptor (M6PR), the major receptor of GrzB, in HCC tissues. In summary, these results suggest that TCS perhaps increases T-cell immunity via promoting the secretion of chemokines and accelerating the entry of GrzB to HCC cells, which highlights the potential role of TCS in anti-tumor immunotherapy.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Tricosantina , Humanos , Tricosantina/farmacologia , Tricosantina/uso terapêutico , Carcinoma Hepatocelular/tratamento farmacológico , Linfócitos T CD8-Positivos/metabolismo , Granzimas , Neoplasias Hepáticas/tratamento farmacológico , Quimiocinas/farmacologia
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