Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.422
Filtrar
1.
Neural Regen Res ; 16(8): 1460-1466, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33433459

RESUMO

Currently, no specific treatment exists to promote recovery from cognitive impairment after a stroke. Dysfunction of the actin cytoskeleton correlates well with poststroke cognitive declines, and its reorganization requires proper regulation of Rho-associated kinase (ROCK) proteins. Fasudil downregulates ROCK activation and protects neurons against cytoskeleton collapse in the acute phase after stroke. An enriched environment can reduce poststroke cognitive impairment. However, the efficacy of environmental enrichment combined with fasudil treatment remains poorly understood. A photothrombotic stroke model was established in 6-week-old male C57BL/6 mice. Twenty-four hours after modeling, these animals were intraperitoneally administered fasudil (10 mg/kg) once daily for 14 successive days and/or provided with environmental enrichment for 21 successive days. After exposure to environmental enrichment combined with fasudil treatment, the number of neurons in the hippocampal CA1 region increased significantly, the expression and proportion of p-cofilin in the hippocampus decreased, and the distribution of F-actin in the hippocampal CA1 region increased significantly. Furthermore, the performance of mouse stroke models in the tail suspension test and step-through passive avoidance test improved significantly. These findings suggest that environmental enrichment combined with fasudil treatment can ameliorate memory dysfunction through inhibition of the hippocampal ROCK/cofilin pathway, alteration of the dynamic distribution of F-actin, and inhibition of neuronal death in the hippocampal CA1 region. The efficacy of environmental enrichment combined with fasudil treatment was superior to that of fasudil treatment alone. This study was approved by the Animal Ethics Committee of Fudan University of China (approval No. 2019-Huashan Hospital JS-139) on February 20, 2019.

2.
J Biol Chem ; : 100274, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33428938

RESUMO

The G protein-coupled receptor GPRC6A regulates various physiological processes in response to its interaction with multiple ligands such as extracellular basic amino acids, divalent cations, testosterone, and the uncarboxylated form of osteocalcin (GluOC). Global ablation of GPRC6A increases the susceptibility of mice to diet-induced obesity and related metabolic disorders. However, given that GPRC6A is expressed in many tissues and responds to a variety of hormonal and nutritional signals, the cellular and molecular mechanisms underlying the development of metabolic disorders in conventional knockout mice have remained unclear. On the basis of our previous observation that long-term oral administration of GluOC markedly reduced adipocyte size and improved glucose tolerance in wild-type mice, we examined whether GPRC6A signaling in adipose tissue might be responsible for prevention of metabolic disorders. We thus generated adipocyte-specific GPRC6A knockout mice, and we found that these animals manifested increased adipose tissue weight, adipocyte hypertrophy, and adipose tissue inflammation when fed a high-fat, high-sucrose diet compared with control mice. These effects were associated with reduced lipolytic activity due to down-regulation of lipolytic enzymes such as adipose triglyceride lipase (ATGL) and hormone-sensitive lipase in adipose tissue of the conditional knockout mice. Given that, among GPR6CA ligands tested, GluOC and ornithine increased the expression of ATGL in cultured 3T3-L1 adipocytes in a manner dependent on GPRC6A, our results suggest that the constitutive activation of GPRC6A signaling in adipocytes by GluOC or ornithine plays a key role in adipose lipid handling and the prevention of obesity and related metabolic disorders.

3.
Ecotoxicol Environ Saf ; 208: 111717, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396048

RESUMO

Antibiotics are currently extensively used in human medicine, animal farming, agriculture and aquaculture, and their residue has become a global environmental problem. However, the effects of antibiotic on other pollutants in aquatic environment are still poorly understood. In this study, the influences of norfloxacin on the residue, degradation and distribution of the herbicides (simazine, atrazine, terbuthylazine, acetochlor and metolachlor) and the enantioselectivity of acetochlor in sediment and water-sediment microcosm system were investigated. Sediment was spiked with norfloxacin and water was contaminated by herbicides to simulate environmental pollution. The amounts of herbicides in water and sediment samples were analyzed within 30 days of cultivation. The results showed that norfloxacin could significantly inhibit the dissipation, lengthen the half-lives and enhance the residues of herbicides in sediment. Take simazine as an example, its half-life significantly increased from 16.1 days to 19.3 days and its residual percentage grew from 24.2% to 30.4% when sediment was contaminated with 5 mg·kg-1 norfloxacin. However, only acetochlor degradation was significantly inhibited by norfloxacin in water-sediment microcosm and the distribution of the herbicides were not affected. Enantioselective degradation of acetochlor was observed both in control and norfloxacin-treated water-sediment system, with R-acetochlor preferential elimination, suggesting the co-existence of norfloxacin had very limited influence on the enantioselectivity. The findings indicated that co-contamination with norfloxacin could increase the persistence of herbicides in aquatic environment, thus increasing the environmental risks to aquatic organisms.

4.
Molecules ; 26(2)2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33451119

RESUMO

Subcritical water treatment has received considerable attention due to its cost effectiveness and environmentally friendly properties. In this investigation, Chinese quince fruits were submitted to subcritical water treatment (130, 150, and 170 °C), and the influence of treatments on the structure of milled wood lignin (MWL) was evaluated. Structural properties of these lignin samples (UL, L130, L150, and L170) were investigated by high-performance anion exchange chromatography (HPAEC), FT-IR, gel permeation chromatography (GPC), TGA, pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), 2D-Heteronculear Single Quantum Coherence (HSQC) -NMR, and 31P-NMR. The carbohydrate analysis showed that xylose in the samples increased significantly with higher temperature, and according to molecular weight and thermal analysis, the MWLs of the pretreated residues have higher thermal stability with increased molecular weight. The spectra of 2D-NMR and 31P-NMR demonstrated that the chemical linkages in the MWLs were mainly ß-O-4' ether bonds, ß-5' and ß-ß', and the units were principally G- S- H- type with small amounts of ferulic acids; these results are consistent with the results of Py-GC/MS analysis. It is believed that understanding the structural changes in MWL caused by subcritical water treatment will contribute to understanding the mechanism of subcritical water extraction, which in turn will provide a theoretical basis for developing the technology of subcritical water extraction.

6.
FEBS Open Bio ; 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33449453

RESUMO

CD47 is a widely expressed self-protection transmembrane protein that functions as a critical negative regulator to induce macrophage-mediated phagocytosis. Overexpression of CD47 enables cancer cells to escape immune surveillance and destruction by phagocytes both in solid tumours and leukaemia. The usefulness of anti-CD47 antibody has been demonstrated in multiple immunotherapies associated with macrophages. However, antigen sinks and toxicity induced by inadvertent binding to normal cells restrict its clinical applications. Here, a novel anti-human CD47 antibody, 4D10, was generated, and its variable regions were grafted onto a human IgG4 scaffold. Compared with the anti-CD47 antibody Hu5F9, the resulting chimeric antibody (c4D10) has consistently demonstrated good tolerance in in vitro and in vivo toxicity studies. Additionally, c4D10 showed effective therapeutic potential through inducing the eradication of human cancer cells. Thus, c4D10 is a promising candidate therapeutic antibody with higher efficacy and reduced side effects as compared to earlier antibodies, and its use may reduce the dose-limiting toxicity of CD47 antagonists for immunotherapy.

8.
J Mater Chem B ; 2021 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33443280

RESUMO

Patients prefer oral drug delivery due to its convenience and noninvasiveness. Nevertheless, a multitude of potentially clinically important drugs will not reach the market or achieve their full potential, due to their low bioavailability and instability in gastric acid. In this study, a novel oral drug delivery system based on poly-cyanoacrylate [a polymer of 2-(2-methoxyethoxy)ethyl-2-cyanoacrylate (MECA)] and hydroxypropyl methylcellulose phthalate (HPMCP) was developed and shown to permit intestinal targeting and sustained drug release. Aspirin [acetylsalicylic acid (ASA)] was selected as a model drug for atherosclerosis treatment. It was physically dissolved in liquid MECA, and the ASA-MECA matrix was then polymerized into a solid drug-loading depot in an HPMCP shell. The delivery of the drug depot in the intestine was achieved with the HPMCP shell; then the polymerized MECA (polyMECA) provided sustained drug release. The polyMECA excipient was not absorbed by the intestine due to its high molecular weight; a fluorescein-labeled assay indicated that it was excreted completely in feces after drug release. The formulation, ASA-polyMECA-HPMCP, showed good intestinal targeting and sustained drug release in vitro and in vivo. Pharmacokinetic studies indicated that this formulation improved the bioavailability of ASA relative to commercially available controls. ASA-polyMECA-HPMCP showed desirable anti-atherosclerosis efficacy in a rabbit model, with significant enhancement of atheromatous lesion stability. Biosafety tests proved the low toxicity of ASA-polyMECA-HPMCP and the polyMECA matrix. We believe that this work has provided a practical and biocompatible system for sustained intestinal drug delivery that can be applied broadly with various drugs for specific therapeutic aims.

9.
J Mater Chem B ; 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33439203

RESUMO

Hydroxyapatite (HAP) is promising for the clinical treatment of bone defects because of its excellent biocompatibility and osteo-conductivity. However, highly porous HAP scaffolds usually exhibit high brittleness and poor mechanical properties, thus organic constituents are usually added to form composite materials. In this work, a highly porous and elastic aerogel made from ultralong HAP nanowires with ultrahigh porosity (∼98.5%), excellent elasticity and suitable porous structure is prepared as the high-performance scaffold for bone defect repair. The highly porous structure of the as-prepared aerogel is beneficial to bone ingrowth and matter/fluid transfer, and the high elasticity can ensure the structural integrity of the scaffold during bone regeneration. Therefore, the HAP nanowire aerogel scaffold can promote the adhesion, proliferation and migration of rat bone marrow derived mesenchymal stem cells (rBMSCs), and elevate the protein expression of osteogenesis and angiogenesis related genes. The in vivo experimental results demonstrate that the HAP nanowire aerogel scaffold is favorable for the ingrowth of new bone and blood vessels, and thus can greatly accelerate bone regeneration and neovascularization. The as-prepared HAP nanowire aerogel scaffold shows promising potential for biomedical applications such as bone defect repair.

10.
Eur J Nutr ; 2021 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-33394121

RESUMO

PURPOSE: Zinc is considered protective against atherosclerosis; however, the association between dietary zinc intake and cardiovascular disease remains debated. We investigated whether dietary zinc intake was associated with coronary artery calcium (CAC) progression in the Multi-Ethnic Study of Atherosclerosis (MESA). METHODS: This analysis included 5186 participants aged 61.9 ± 10.2 years (48.8% men; 41.3% white, 25.0% black, 21.6% Hispanic, and 12.1% Chinese American) from the MESA. Dietary zinc intake was assessed by a self-administered, 120-item food frequency questionnaire at baseline (2000-2002). Baseline and follow-up CAC were measured by computed tomography. CAC progression was defined as CAC > 0 at follow-up for participants with CAC = 0 at baseline; and an annualized change of 10 or percent change of ≥ 10% for those with 0 < CAC < 100 or CAC ≥ 100 at baseline, respectively. RESULTS: Dietary zinc intake was 8.4 ± 4.5 mg/day and 2537 (48.9%) of the included participants had CAC at baseline. Over a median follow-up of 3.4 years (25th-75th percentiles = 2.0-9.1 years), 2704 (52.1%) participants had CAC progression. In the fully adjusted model, higher dietary zinc was associated with a lower risk of CAC progression in both men (hazard ratio [HR] 0.697, 95% confidence interval [CI] 0.553-0.878; p = 0.002) and women (HR 0.675; 95% CI 0.496-0.919; p = 0.012, both comparing extreme groups). Furthermore, such an inverse association was attributable to dietary zinc intake from non-red meat (p < 0.05), rather than red meat sources (p > 0.05). CONCLUSIONS: In this multiethnic population free of clinically apparent cardiovascular disease, higher dietary zinc intake from non-red meat sources was independently associated with a lower risk of CAC progression. CLINICAL TRIAL REGISTRATION NUMBER: The MESA trial was registered at clinicaltrials.gov as NCT00005487.

11.
Arterioscler Thromb Vasc Biol ; 41(1): 491-500, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33115269

RESUMO

OBJECTIVE: To investigate whether low-carbohydrate diets (LCDs) were associated with coronary artery calcium (CAC) progression. Approach and Results: We included the participants who completed computed tomography assessment of baseline CAC in 2000 to 2001 (year 15) and follow-up (year 20 or 25) and food frequency questionnaire (years 0, 7, and 20) in the CARDIA study (Coronary Artery Risk Development in Young Adults). CAC progression was defined as CAC >0 at follow-up among participants with baseline CAC of 0 and an annualized change of 10 or percent change of ≥10% for those with 0

12.
Langmuir ; 37(1): 417-427, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33347295

RESUMO

Although loose nanofiltration membranes have been extensively studied for dye desalination, high-throughput membranes with antifouling and antibacterial properties are still highly needed. In this study, a zwitterion-modified molybdenum disulfide (MoS2) dual-layer loose nanofiltration membrane was prepared with the integration of antibacterial, antifouling, and high-flux properties. To be specific, MoS2 nanosheets were loaded on a polyacrylonitrile ultrafiltration membrane through pressure-assisted self-assembly. Then, poly (sulfobetaine methacrylate) (PSBMA) was coated on the surface of the MoS2 membrane via a simple polydopamine (PDA)-assisted one-step codeposition to prepare PSBMA/PDA/MoS2 nanofiltration membranes. Elemental and morphological analyses confirmed the formation of the MoS2 layer and PSBMA/PDA coating. In addition, the effect of the PSBMA amount and codeposition time on surface properties and membrane performances was investigated. Under optimum conditions, the as-prepared membrane showed excellent water permeance of 262 LMH/bar with good dye rejection (99.8% for methylene blue) and salt permeability, as well as excellent antifouling and antibacterial properties benefiting from the synergy of PSBMA/PDA coating layers and MoS2 layers.

13.
Cell Res ; 31(1): 80-93, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32669607

RESUMO

Whether glucose is predominantly metabolized via oxidative phosphorylation or glycolysis differs between quiescent versus proliferating cells, including tumor cells. However, how glucose metabolism is coordinated with cell cycle in mammalian cells remains elusive. Here, we report that mammalian cells predominantly utilize the tricarboxylic acid (TCA) cycle in G1 phase, but prefer glycolysis in S phase. Mechanistically, coupling cell cycle with metabolism is largely achieved by timely destruction of IDH1/2, key TCA cycle enzymes, in a Skp2-dependent manner. As such, depleting SKP2 abolishes cell cycle-dependent fluctuation of IDH1 protein abundance, leading to reduced glycolysis in S phase. Furthermore, elevated Skp2 abundance in prostate cancer cells destabilizes IDH1 to favor glycolysis and subsequent tumorigenesis. Therefore, our study reveals a mechanistic link between two cancer hallmarks, aberrant cell cycle and addiction to glycolysis, and provides the underlying mechanism for the coupling of metabolic fluctuation with periodic cell cycle in mammalian cells.

14.
Huan Jing Ke Xue ; 42(1): 55-64, 2021 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-33372457

RESUMO

The characterization and source apportionment of atmospheric volatile organic compounds (VOCs) in Tianjin in 2019 were investigated based on high-resolution online monitoring data observed at an urban site in Tianjin. The results showed that the average annual concentration of VOCs was 48.9 µg·m-3, and seasonal concentrations followed with winter (66.9 µg·m-3) > autumn (47.9 µg·m-3) > summer (42.0 µg·m-3) > spring (34.6 µg·m-3). The chemical compositions of the VOCs were alkanes, aromatics, alkenes, and alkynes, which accounted for 65.0%, 17.4%, 14.6%, and 3.0% of the VOCs concentrations on average, respectively. The proportion of alkanes, aromatics, and alkynes was the highest in autumn, summer, and winter, respectively, while a higher alkenes proportion was observed in summer and winter. The ozone formation potential contribution of alkanes, alkenes, aromatics, and alkynes in spring and summer was 16.9%, 48.6%, 33.5%, and 1.0%, respectively, and the species with higher contributions were ethene, propylene, m,p-xylene, 1,2,3-trimethylbenzene, toluene, isoprene, trans-2-butene, cis-2-pentene, o-xylene, and m-ethyltoluene. During autumn and winter, the aromatics contributed as much as 91.5% to the secondary organic aerosol (SOA) formation potential, and o-xylene, toluene, m,p-xylene, ethylbenzene, o-ethyltoluene, and benzene were the main contributing species. Positive matrix factorization was applied to estimate VOCs source contributions, and automobile exhaust, liquefied petroleum gas/natural gas (LPG/NG) and gasoline evaporation, solvent usage, petrochemical industrial emissions, combustion, and natural sources were identified as major sources of VOCs in spring and summer, accounting for 29.2%, 19.9%, 16.4%, 10.3%, 7.3%, and 6.6%, respectively. While in autumn and winter, the contributions of LPG/NG and gasoline evaporation, automobile exhaust, combustion, solvent usage, and petrochemical industrial emissions were 32.4%, 21.9%, 18.5%, 13.3%, and 8.4%, respectively. Compared to the source contributions in spring and summer, a significant increase was observed for LPG/NG and combustion emission of 62.8% and 153.4%, respectively, and other sources decreased by 18.4%-25.0% in autumn and winter. Source composition spectrums showed that the petrochemical industry and solvent usage were the main emission sources of alkenes and aromatics in spring and summer, and combustion and solvent usage were the main emission sources of aromatics in autumn and winter. Thus, focus should be played on the petrochemical industry and solvent usage in spring and summer and on combustion and solvent usage in autumn and winter to further prevent and control ozone and SOA in Tianjin.

15.
Bioresour Technol ; 320(Pt A): 124280, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33120060

RESUMO

The main purpose of this study is to isolate and purify oligotrophic denitrifying bacteria, Acinetobacter sp. FYF8, so as to study the denitrification capacity and characteristics in response to oligotrophic ecosystem. The RSM showed that the best denitrification efficiency was 97.90% under 7.58 pH, 20.69 °C temperature, and 2.83 C/N ratio. Nitrogen balance experiments showed that the nitrogen gas conversion ratio was 39.88, 68.85, and 78.79% at 2.0, 2.5, and 3.0 C/N ratio, respectively. According to 3D-EEM, tyrosine, tryptophan and aromatic protein were the metabolites produced by strain FYF8. The concentration of polysaccharide (PS) and proteins (PN) in different types of extracellular polymeric substances (EPS) and the variation trend were quantitatively studied. Different functional groups such as CH2, C = O, and C-O-C was characterized by FTIR. These findings indicated that the denitrification strategy of strain FYF8 was related to EPS, which might be a reserve carbon storage in carbon scarcity.


Assuntos
Acinetobacter , Desnitrificação , Carbono , Ecossistema , Nitrificação , Nitrogênio
16.
Am J Cardiol ; 140: 13-19, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33159905

RESUMO

We aimed to explore the utility of multiple biomarkers with GRACE risk stratification for non-ST-elevation myocardial infarction (NSTEMI). A total of 1,357 patients diagnosed with NSTEMI were enrolled in this study at multiple medical centers in Tianjin, China. The outcomes were 1-year all-cause death and major adverse cardiac events (MACE: all-cause death, hospital admission for unstable angina, hospital admission for heart failure, nonfatal recurrent myocardial infarction, and stroke). C-index, net reclassification improvement (NRI), and integrated discrimination improvement (IDI) were calculated to verify that the biomarkers improve the predictive accuracy of the GRACE score. A total of 57 participants died, while 211 participants experienced 231 MACEs during follow-up (mean: 339 days). For all-cause death, the combination of N-terminal pro-B-type natriuretic peptide (NT-proBNP) and D-dimer improved the predictive accuracy of GRACE the most, with C-index, IDI, and NRI values of 0.88, 0.085, and 1.223, respectively. For MACE, trigeminal combination of NT-proBNP, fibrinogen, and D-dimer resulted in C-index, IDI, and NRI values of 0.80, 0.079, and 0.647, respectively. As a result, NT-proBNP, D-dimer, fibrinogen, and GRACE comprise a new scoring system for assessing 1-year clinical events. Kaplan-Meier analysis revealed a significant increase in 1-year mortality (score ≥3.85 vs <3.85, p < 0.0001) and 1-year MACE (score ≥1.72 vs <1.72, p < 0.0001) between different score groups. In conclusion, the combination of NT-proBNP and D-dimer added prognostic value to GRACE for all-cause death. Combining NT-proBNP, fibrinogen, and D-dimer increased the prognostic value of GRACE for MACE. This newly developed scoring system is strongly correlated with all-cause mortality and MACE, and can be easily utilized in clinical practice.

17.
J Colloid Interface Sci ; 583: 89-99, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32980683

RESUMO

Fingerprints are widely studied due to their unique shape and lifelong properties. The latent fingerprint (LFP) visualization is critical in identifying crime scenes and personal information. At present, the powder dusting method for LFP detection is favored due to its environmental friendliness and nontoxicity. However, this method has low resolution, low sensitivity, and large background interference. To address these shortcomings, the red-emitting Sr2MgMoO6:xEu3+ (x = 0-0.50) phosphors were synthesized using the solid-state reaction process. The products were systematically studied through the structural phase, luminescent property, decay curve, and color purity. Sr2MgMoO6:xEu3+ phosphors were monitored at 596 nm and exhibited a commendable broad excitation band between 250 and 475 nm. This result indicated that the disadvantage of the poor absorption of commercial red phosphors (Y2O2S:Eu3+) in the near-ultraviolet region was overcome. Under excitation at 393 nm, the synthesized Sr2MgMoO6:Eu3+ phosphor exhibited intense red light at 596 and 616 nm, due to the 5D0 â†’ 7F1 and 5D0 â†’ 7F2 transitions of Eu3+ ions. The optimal concentration for the Sr2MgMoO6:xEu3+ phosphor was x = 20 mol%, and the concentration quenching effect was ascribed to the dipole-dipole interaction. The Commission International del'Eclairage (CIE) chromaticity coordinates of Sr2MgMoO6:Eu3+ were (0.643, 0.356), and the color purity was 99.8%. Furthermore, the fluorescent LFP images developed by Sr2MgMoO6:0.20Eu3+ phosphors were well visualized, and level 1-3 details were well identified with high resolution, contrast, sensitivity, and selectivity. The obtained results suggested that the Sr2MgMoO6:Eu3+ phosphor can be applied for LFP detection.

19.
Drug Deliv ; 28(1): 19-36, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33336609

RESUMO

In recent years, nanocrystal technology has been extensively investigated. Due to the submicron particle size and unique physicochemical properties of nanocrystals, they overcome the problems of low drug solubility and poor bioavailability. Although the structures of nanocrystals are simple, the further development of these materials is hindered by their stability. Drug nanocrystals with particle sizes of 1∼1000 nm usually require the addition of stabilizers such as polymers or surfactants to enhance their stability. The stability of nanocrystal suspensions and the redispersibility of solid nanocrystal drugs are the key factors for the large-scale production of nanocrystal preparations. In this paper, the factors that affect the stability of drug nanocrystal preparations are discussed, and related methods for solving the stability problem are put forward.

20.
Int J Cancer ; 148(1): 77-89, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32638381

RESUMO

Evidence from animal models suggests that dietary fatty acids have both anticancer and tumor-promoting effects. Whether dietary fatty acids are associated with colorectal cancer (CRC) in humans remains inconclusive. We investigated associations between dietary fatty acids and risk of CRC among 59 986 men who participated in the Shanghai Men's Health Study (SMHS), an ongoing population-based prospective cohort study. We identified 876 incident CRC cases in the SMHS during a mean follow-up of 9.8 years. Associations between dietary fatty acid intake and CRC risk were evaluated by Cox proportional hazard regression analyses. Consumption of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) was not significantly associated with CRC risk. Multivariate hazard ratios (HRs) and respective 95% confidence intervals (CIs) for Quartile 4 vs Quartile 1 were 0.92 (0.74-1.14; Ptrend = 0.47) for SFA, 0.95 (0.79-1.16; Ptrend = 0.74) for MUFA and 1.18 (0.95-1.46; Ptrend = 0.21) for PUFA. No significant associations were found for total n-6 PUFA or total n-3 PUFA. Additionally, we performed a meta-analysis to summarize results from the present study and 28 reports from 26 additional cohorts, which supported the overall null association between dietary fatty acid intake and CRC risk among men. Docosahexanoic acid and eicosapentaenoic acid were associated with 11% to 12% reduced risk, and linoleic acid a 19% increased risk, of CRC in the meta-analysis of combined sexes. In conclusion, this population-based prospective study and meta-analysis of cohort studies found little evidence that dietary fatty acid intake was associated with risk of CRC in men.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA