Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.765
Filtrar
1.
Biomed Environ Sci ; 33(1): 11-18, 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-32029054

RESUMO

Objectives: The aims of this study were to assess the associations between parity and metabolic syndrome (MetS) and its components and to evaluate the effects of body mass index (BMI) on these associations. Methods: A total of 5,674 women were enrolled from Jidong and Kailuan communities (Tangshan, Hebei) in Northern China. All participants completed standardized questionnaires, physical examination, and biochemical measurements. Logistic regression analysis was used to test the associations. Results: Compared with women with parity of one, nulliparous women had decreased odds ratios ( ORs ); those with parity of two had odds of abdominal obesity [ OR= 1.45, 95% confidence interval ( CI) 1.17-1.81, P < 0.001], high blood pressure ( OR= 1.26, 95% CI: 1.03-1.54, P = 0.025), elevated fasting glucose levels ( OR= 1.36, 95% CI: 1.03-1.79, P = 0.029), and MetS ( OR= 1.39, 95% CI: 1.13-1.73, P = 0.002); and those with parity of three or more had increased odds of elevated triglyceride levels ( OR= 1.42, 95% CI: 1.04-1.94, P = 0.027) and MetS ( OR= 1.50, 95% CI: 1.10-2.05, P = 0.011) after complete adjustment for confounders. Furthermore, BMI and age subgroups partially modified the associations between parity and MetS and its components. Conclusions: Parity is positively associated with MetS and select components in women. BMI is an important modifier involved in the associations between parity and MetS.

2.
ACS Chem Neurosci ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32049477

RESUMO

Mitophagy is an important process for removing damaged mitochondria in cells, the dysfunction of which has been directly linked to an increasing number of neurodegenerative disorders. However, the details of mitophagy in prion diseases still need to be deeply explored. In this study, we identified more autophagosomes and large swelling mitochondria structures in the prion infected cultured cell line SMB-S15 by transmission electron microscopy, accompanying with the molecular evidences of activated autophagic flux. Western blots illustrated that the levels of Pink1 and Parkin, particularly in the mitochondrial fraction, were increased in SMB-S15 cells, whereas the levels of mitochondrial membrane proteins TIMM44, TOMM20 and TIMM23 were decreased. The amount of the whole polyubiquitinated proteins decreased but that of phosphor-polyubiquitinated proteins increased in SMB-S15 cells. The level of MFN2 in SMB-S15 cells were downregulated but its polyubiquitinated form upregulated. Knock-down of the expressions of Pink1 and Parkin by the individual SiRNAs in SMB-S15 cells reduced autophagic activity but seemed not to influence the expressions of TOMM20 and TIMM23. Moreover, we also demonstrated that the brain levels of Pink1 and Parkin in the mice infected with scrapie strains 139A and ME7 were remarkably increased at the terminal stage of the disease by Western blot and immunohistochemical (IHC) assays. Immunofluorescent assays (IFA) revealed that Pink1 signals widely colocalized with GAFP- Iba1- and NeuN positive cells in the brains of scrapie infected mice. IHC assays with serial sections of the brain tissues infected with agents 139A and ME7 mice showed more Pink1 and Parkin positive cells located at the areas with more PrPSc deposit. These results suggest that an activated mitophagy in prion infected cells and prion infected experimental mice, probably via enhanced Pink-Parkin pathway.

3.
Nanotechnology ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32053800

RESUMO

Gold nanoparticles (AuNPs) modification shows great advantages in improving the antioxidant activity of nanoCeO2. However, the improved effect of AuNPs modification become smaller and even results the decrease of antioxidant ability due to the severe aggregation with the increase of nanomaterials' concentration. Additionally, the effects of photo-properties of AuNPs on the antioxidant activity of nanoCeO2 have not been studied. In response to these problems, a core-shell shaped Au@CeO2 was synthesized which took Au nanorods (AuNRs) as carriers and with a layer of CeO2 nanoparticles (NPs) coating. The antioxidant activity of Au@CeO2 was evaluated by UV-vis method in MV-Fenton system. Results showed that AuNRs could improve the antioxidant activity of nanoCeO2 due to the increase of Ce3+ amount on the surface of nanoCeO2, and the enhancing effect was remained across the whole experimental concentration range due to the good dispersibility of AuNRs. Additionally, a further increase of the antioxidant ability of Au@CeO2 has been found with 5 min visible light irradiation, and continuous irradiation during a 25 min time reaction resulted in more obvious enhanced antioxidant ability. This phenomenon was attributed to the localized surface plasmon resonance (LSPR) of AuNRs triggered by photons which induced charge transfer from AuNRs to nanoCeO2, thus making the recycle become easier between Ce3+ and Ce4+.

4.
Bioresour Technol ; 302: 122903, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32018084

RESUMO

Screening for highly efficient microalgae is an important technique for improving treatment efficiency. In this study, eight species of microalgae (five Scenedesmus and three Desmodesmus) were isolated from water and soil in the Hexi Corridor region, China, and identified by 18S rRNA gene sequence analysis. Scenedesmus sp. HXY2 grew well under high total organic carbon and ammonia conditions and had the highest nutrient removal efficiency (>95%). On day 12, the biomass of Scenedesmus sp. HXY2 was 7.2 × 106 cells mL-1. The lipid content and productivity of this species were 15.56% and 5.67 mg L-1 day-1, respectively. The proportion of unsaturated fatty acids (60.07%) indicated that the lipids of Scenedesmus sp. HXY2 were suitable for biodiesel production. Scenedesmus sp. HXY2 showed great potential for growth in wastewater with high ammonia and organic contents to simultaneously purify wastewater and produce lipids.

5.
Sci Rep ; 10(1): 2749, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32066838

RESUMO

A high-quality cadmium manganese tellurium (Cd0.9Mn0.1Te: V or VCMT) crystal was successfully grown via modified Te solution vertical Bridgman method with vanadium doping. The crystal structure and quality were evaluated by powder X-ray diffraction analysis. An infrared transmission spectroscope measured the transmittance of the crystal at 64%, which would suggest that the grown crystal possessed high purity and crystallinity. Ultraviolet-visible-near-infrared spectroscopy analysis obtained the forbidden band width of approximately 1.577 eV. The current-voltage test indicated that the VCMT crystal had a high resistivity of 2.07 × 1010 Ω·cm. Mechanical properties were measured by a Vickers microhardness tester. Crack surface morphology around the indentation was recorded. Furthermore, mechanical properties, such as microhardness, fracture toughness, brittleness index and yield strength were investigated and discussed. The thermal stability of the VCMT single crystal was determined by thermogravimetric analysis. A VCMT detector was fabricated with planar configuration structure, which showed a resolution of 11.62% of the 241Am at 59.5 keV peak.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32067169

RESUMO

In this study, a photocatalyst S-doped WO3 was successfully synthesized by the hydrothermal method. The prepared undoped and S-doped WO3 samples were then characterized by XRD, SEM, XPS, and UV-vis DRS. The results showed that the band gap energy of S-doped WO3 was lower than that of the undoped WO3, which led to a better absorption of visible light. Furthermore, the results of XPS analysis suggested that the doping with S element resulted in an increase in lattice oxygen vacancies on the surface of S-WO3, which could effectively improve the photocatalytic activity. The photocatalytic performance of the S-WO3 samples were evaluated by the measurement of methylene blue (MB) degradation under visible light irradiation. The experimental results demonstrated that S-doped WO3 sample exhibited a much better photodegradation performance compared to undoped WO3, with the maximum MB removal efficiency of 78.7% for the 5% S-WO3 sample. Based on the above results, the mechanisms of photodegradation of MB by S-WO3 were discussed.

7.
J Thorac Imaging ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32032250

RESUMO

OBJECTIVE: We sought to introduce a localization procedure (methylene blue-stained N-butyl cyanoacrylate and N-octyl cyanoacrylate glue) in localizing pulmonary small nodules and ground-glass opacities before thoracoscopic resection, and to evaluate its efficacy. METHODS: A total of 20 patients with pulmonary small nodules and/or ground-glass opacities, who underwent video-assisted thoracoscopic surgery from August 1, 2017 to March 1 2018, were included in the study. RESULTS: A total of 24 lesions in 20 patients underwent blue-stained glue localization. The success rate of localization was 100%, with a mean dose of 0.04±0.01 mL blue dye and 1 mL glue used for each lesion. The average time for the whole localization procedure was 15.4±6.3 minutes. All lesions were intraoperatively localized by visual inspection in combination with palpation. The complications related to the localization procedure included mild pneumothorax occurring in 9 patients and minor pulmonary hematoma in 4 patients. No pain or distress was reported. CONCLUSIONS: Blue-stained glue injection is technically feasible and safe to localize pulmonary small nodules and ground-glass opacities before thoracoscopic resection.

8.
Exp Gerontol ; : 110843, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32045633

RESUMO

Scutellaria baicalensis Georgi flowers is rich in flavonoids resources but not effectively exploited. This study aimed to investigate the anti-aging effects and potential mechanism of Scutellaria baicalensis Georgi flowers extract (SFE). The chemical components of the SFE were analyzed by UPLC-MS and the anti-aging effects of SFE were investigated in d-galactose (d-gal) induced aging rats by behavior examination and biochemical indexes, and the potential anti-aging mechanism of SFE were explored by 1H NMR-based liver metabolomics. Chemical composition research showed that 19 flavonoids were identified in SFE, and pharmacological research showed that SFE could significantly ameliorate spatial learning and memory ability. SFE could significantly regulate malondialdehyde (MDA), superoxide dismutase (SOD) and advanced glycation end products (AGEs). It also ameliorated the pathological abnormalities in liver. Additionally, anti-aging mechanism of SFE showed that total of 10 potential biomarkers were found by metabolomics techniques, which involved in 6 metabolic pathways. Among them, SFE could significantly increased the levels of d-glutamine and d-glutamate. Furthermore, the levels of glutamine and glutamate, and the levels of the key amino acids, enzymes and final product in the synthesis process of glutathione (GSH) were quantitatively determined in the liver by commercial kits and enzyme-linked immunosorbent assay. These results indicated that regulation of the glutamine-glutamate metabolic pathway is involved in the anti-aging effect of SFE in d-gal induced aging rats.

9.
Sci Total Environ ; 715: 136810, 2020 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-32007878

RESUMO

Toxicity, bioavailability, and mobility of a metal depend on its speciation rather than its total concentration. In this discussion, we emphasize the need for field-based metal speciation analysis methods, given the flaws of traditional techniques. We express a science-based opinion that the diffusive gradients in thin-films (DGT) is a promising and powerful technique for performing in-situ measurement of metal species or speciation in different environmental samples. Several limitations and challenges in further application of DGT are also highlighted in this discussion.

10.
ACS Chem Neurosci ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32011849

RESUMO

About 16% of the world's population has major depressive disorder. Traditional antidepressants have slow effect rates and low response rates. Many studies have shown that low doses of ketamine can produce rapid and effective antidepressant effects. However, its mechanism of action needs further exploration. Lipopolysaccharide (LPS) was used to establish a depression model in rats and PC12 nerve cells were used for in vitro experiments. (2,4)-Dimethoxybenzylidene anabaseine dihydrochloride (GTS-21), a specific agonist of α7 nicotinic acetylcholine receptors (α7 nAChRs), was used to compare the rapid antidepressant effect of ketamine. Different doses of α7 nAChR antagonist methyllycaconatine (MLA) and α7 nAChR-siRNA were used to interfere with the protective effects of ketamine on neuroinflammation in rats and PC12 cells, respectively. MLA intervention downregulated the anti-inflammatory effects of ketamine and decreased the effects of ketamine on behavior, synaptic plasticity, and Nissl bodies in the neuronal cells. Moreover, the dose of MLA was positively correlated with the inhibitory effect in rat hippocampi and the protective effects of GTS-21 were consistent with ketamine. These results demonstrated that low-dose ketamine could produce neuroprotective effects by activating the α7 nAChR-mediated cholinergic anti-inflammatory pathway (CAP) in depression, resulting in a rapid antidepressant effect.

11.
Environ Sci Technol ; 54(3): 1484-1492, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31927953

RESUMO

Dissolved organic nitrogen (DON) represents a unique challenge in prechlorinated raw water distribution systems (PRWDSs) because of its contribution to the formation of harmful nitrogen-disinfection byproducts, influence upon biogeochemical processes, and unclear molecular characteristics. Here, Fourier transform ion cyclotron resonance mass spectrometry in combination with high-throughput sequencing was applied to elucidate the molecular changes of DON and biofilm microbial communities in a PRWDS in Yixing, China. Our study revealed that dynamic characteristics of DON are significantly correlated with the biofilm. The accumulation of refractory lignin-like compounds and CnHmOpN1 contributes to the higher recalcitrance molecular characteristics of DON in the effluent associated with Alphaproteobacteria, Planctomycetes, and Bacteroidetes. Additionally, with the help of prechlorination, the biofilm may change the DON characteristics and lead to higher oxygenation, higher m/z, and lower saturation during transportation. Despite the promotion of CnHmOpN1 and CnHmOpN3 at the early stage, we suggest that appropriate concentration of chlorine can add to the front end of raw water distribution pipes. Prechlorination may control the nitrification process and stabilize the rapid growth of diversity and concentration of low molecular weight DON, especially the refractory CnHmOpN1 in the effluent, which may help to improve treatment efficiency of drinking water treatment plants.

12.
Appl Microbiol Biotechnol ; 104(4): 1785-1793, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31900555

RESUMO

Avian leukosis virus subgroup J (ALV-J) is an important pathogen for various neoplasms and causes significant economic losses in the poultry industry. Serological detection of specific antibodies against ALV-J infection is important for successful clinical diagnosis. Here, a 293F stable cell line was established to stably express gp85 protein. In this cell line, gp85 protein was expressed at approximately 30 mg/L. A subgroup-specific indirect enzyme-linked immunosorbent assay (iELISA) was developed using ALV-J gp85 protein as coated antigen to detect antibodies against ALV-J. The sensitivity of the iELISA (1:51200 diluted in serum) was 16 times more than that of indirect immunofluorescence assay (IFA; 1:3200 diluted in serum). Moreover, there was no crossreactivity with antibodies against other common avian viruses and other avian leukosis virus subgroups, such as subgroups A and B. The practicality of the iELISA was further evaluated by experimental infection and clinical samples. The results from experimental infection indicated that anti-ALV-J antibodies were readily detected by iELISA as early as 4 weeks after ALV-J infection, and positive antibodies were detected until 20 weeks, with an antibody-positive rate of 11.1% to 33.3%. Moreover, analysis of clinical samples showed that 9.49% of samples were positive for anti-ALV-J antibodies, and the concordance rate of iELISA and IFA was 99.24%. Overall, these results suggested that the subgroup-specific iELISA developed in this study had good sensitivity, specificity, and feasibility. This iELISA will be very useful for epidemiological surveillance, diagnosis, and eradication of ALV-J in poultry farms.

13.
BMJ Open ; 10(1): e032840, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31911517

RESUMO

OBJECTIVE: The metabolic syndrome is a major risk factor for cardiovascular disease. Little information exists on the prevalence of the metabolic syndrome at high-altitude areas in China. We aimed to estimate the prevalence of metabolic syndrome and its individual components at high altitude. METHODS: A cross-sectional survey of 5053 adults living in Derong from elevation of 2060 to 3820 m was carried out in 2013. Metabolic syndrome was defined according to the Chinese Diabetes Society criteria. RESULTS: The overall prevalence of metabolic syndrome was 3.6% (5.9% in men and 1.8% in women) in Derong, China. Obesity and hypertension were more prevalent among adults than dyslipidaemia and hyperglycaemia at high altitude. The prevalence of metabolic syndrome was higher in township than countryside residents (6.6%, 11.9% in men and 1.5% in women vs 3.0%, 4.6% in men and 1.8% in women). Men with age 30-59 years old had a much higher prevalence of metabolic syndrome than women. Men, township, middle and old age residents had a higher risk of metabolic syndrome. The risk of obesity and dyslipidaemia decreased and the risk of hypertension increased in very high altitude (≥3000 m) residents. CONCLUSION: In Derong, despite the relatively low prevalence of metabolic syndrome, hypertension and obesity are more prevalent in adult residents. And metabolic syndrome is more concentrated in township and male residents. These people also have a higher risk of metabolic syndrome. Therefore, it is necessary to develop a national strategy for the prevention and treatment of metabolic syndrome for high-risk population at high altitude in China.

14.
J Periodontol ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31950496

RESUMO

BACKGROUND: Interleukin-22 (IL-22) exerts extensive biological effects, playing both protective and pathological roles in autoimmune and infectious diseases. However, the specific role and mechanism of IL-22 in the pathogenesis of periodontitis have not been clarified. The aim of this study was to analyze the possible roles of IL-22 in the osteoclastogenesis and osteogenesis of periodontitis. METHODS: Human periodontal ligament fibroblasts (hPDLFs) were treated with IL-22 and/or lipopolysaccharide from Porphyromonas gingivalis (Pg-LPS), and the mRNA and protein expression of RANKL and OPG were measured by qRT-PCR and Western blotting, respectively. Western blotting was also used to examine the phosphorylated and total protein expression of MAPK signaling molecules. The role of the MAPK pathway in osteoclastogenesis marker expression was further confirmed by inhibition assays. For osteogenic assays, the mRNA expression of osteoblastic markers was quantified by qRT-PCR, the ALP activity of hPDLFs was measured by an ALP assay, and the mineralized nodules formed by hPDLFs were determined by Alizarin Red S staining. RESULTS: IL-22 promoted the expression of RANKL in hPDLFs via the MAPK signaling pathway and further upregulated RANKL expression together with Pg-LPS via the p38 MAPK pathway. IL-22 could enhance the ALP activity and mineralized nodule formation of hPDLFs in the early period of osteogenic induction, while exhibiting no profound effect on the expression of osteoblastic markers. CONCLUSION: IL-22 plays regulatory roles in bone homeostasis, and it is likely to contribute to osteoclastogenesis as a proinflammatory cytokine in the pathogenesis of periodontitis. This article is protected by copyright. All rights reserved.

15.
BMC Surg ; 20(1): 13, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31937295

RESUMO

BACKGROUND: The level VI lymph nodes are anatomically connected to the level VII lymph nodes and papillary thyroid carcinoma (PTC) can metastasis to both regions. The aim of this study was to identify clinicopathologic factors associated with level VII lymph node metastasis. METHODS: Between March 2015 and September 2016, a total of 275 consecutive patients were operated on for PTC with prophylactic level VII dissection at the Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, ZheJiang, China. Patients with PTC were performed via video-assisted approach. Multivariate logistic regression analysis was performed to evaluate the associations between clinicopathologic factors. RESULTS: Of the 275 subjects enrolled in this study, 79 patients (28.73%) showed lymph node metastasis to the level VII. The multivariate logistic regression analysis showed ultrasonography-positive lymph nodes (p < 0.001), the location of primary carcinoma (p = 0.002) and hashimoto thyroiditis (HT) (p = 0.04) were associated with level VII lymph node metastasis. CONCLUSIONS: Based on the results of our study, we considered central-compartment lymph node dissection (CLND) as an integral strategy. On the basis of surgery safety, transcervical level VII lymph node dissection could be considered for PTC patients with high risk factors such as ultrasonography-positive lymph nodes, tumor located in middle and lower thirds of the thyroid lobe and the patients without HT. In future, prognostic significance of level VII lymph node dissection should be evaluated through long-term surveillance.

16.
AAPS J ; 22(2): 28, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31938899

RESUMO

It has been proposed that the binding-site barrier (BSB) for antibody-drug conjugates (ADCs) can be overcome with the help of antibody coadministration. However, broad utility of this strategy remains in question. Consequently, here, we have conducted in vivo experiments and pharmacokinetics-pharmacodynamics (PK-PD) modeling and simulation (M&S) to further evaluate the antibody coadministration hypothesis in a quantitative manner. Two different Trastuzumab-based ADCs, T-DM1 (no bystander effect) and T-vc-MMAE (with a bystander effect), were evaluated in high-HER2 (N87) and low-HER2 (MDA-MB-453) expressing tumors, with or without the coadministration of 1, 3, or 8-fold higher Trastuzumab. The tumor growth inhibition (TGI) data was quantitatively characterized using a semi-mechanistic PK-PD model to determine the nature of drug interaction for each coadministration regimen, by estimating the interaction parameter ψ. It was found that the coadministration strategy improved ADC efficacy under certain conditions and had no impact on ADC efficacy in others. The benefit was more pronounced for N87 tumors with very high antigen expression levels where the effect on treatment was synergistic (a synergistic drug interaction, ψ = 2.86 [2.6-3.12]). The benefit was diminished in tumor with lower antigen expression (MDA-MB-453) and payload with bystander effect. Under these conditions, the coadministration regimens resulted in an additive or even less than additive benefit (ψ ≤ 1). As such, our results suggest that while antibody coadministration may be helpful for ADCs in certain circumstances, one should not broadly apply this strategy to all the scenarios without first identifying the costs and benefits of this approach.

17.
Clin Sci (Lond) ; 134(2): 103-122, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31898747

RESUMO

Alcohol consumption causes renal injury and compromises kidney function. The underlying mechanism of the alcoholic kidney disease remains largely unknown. In the present study, an alcoholic renal fibrosis animal model was first employed which mice received liquid diet containing alcohol for 4 to 12 weeks. The Masson's Trichrome staining analysis showed that kidney fibrosis increased at week 8 and 12 in the animal model that was further confirmed by albumin assay, Western blot, immunostaining and real-time PCR of fibrotic indexes (collagen I and α-SMA). In vitro analysis also confirmed that alcohol significantly induced fibrotic response (collagen I and α-SMA) in HK2 tubular epithelial cells. Importantly, both in vivo and in vitro studies showed alcohol treatments decreased Smad7 and activated Smad3. We further determined how the alcohol affected the balance of Smad7 (inhibitory Smad) and Smad3 (regulatory Smad). Genome-wide methylation sequencing showed an increased DNA methylation of many genes and bisulfite sequencing analysis showed an increased DNA methylation of Smad7 after alcohol ingestion. We also found DNA methylation of Smad7 was mediated by DNMT1 in ethyl alcohol (EtOH)-treated HK2 cells. Knockdown of Nox2 or Nox4 decreased DNMT1 and rebalanced Smad7/Smad3 axis, and thereby relieved EtOH-induced fibrotic response. The inhibition of reactive oxygen species by the intraperitoneal injection of apocynin attenuated renal fibrosis and restored renal function in the alcoholic mice. Collectively, we established novel in vivo and in vitro alcoholic kidney fibrosis models and found that alcohol induces renal fibrosis by activating oxidative stress-induced DNA methylation of Smad7. Suppression of Nox-mediated oxidative stress may be a potential therapy for long-term alcohol abuse-induced kidney fibrosis.

18.
Int J Nurs Pract ; : e12817, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31985129

RESUMO

BACKGROUND AND AIM: To our knowledge, the practice of transitional care was not common in mainland China. This study was designed to test the intervention effects of a specific dose (4 weeks) of an Omaha-based system transitional care programme for Chinese patients suffering from rheumatoid arthritis. METHODS: The intervention group adopted a 4-week nurse-led transitional care intervention based on the Omaha System. The control group used a comparable length of routine care. The outcome indexes were a Chinese version of the Arthritis Self-Efficacy Scale-8, Health Assessment Questionnaire-Disability Index, and hospital readmission rates. Data were collected from June 2018 to December 2018. RESULTS: Finally, 88 patients (44 in the intervention group and 44 in the control group) entered the data analysis. Baseline sociodemographic data for both groups were not found to be statistically significant. The self-efficacy and HAQ-DI of the intervention group were significantly different from the control group. Although the readmission rates of the intervention group were lower than that of the control group, it was not statistically significant. CONCLUSIONS: This 4-week transitional care intervention dose is sufficient to provide evidence for improving self-efficacy to implement disease management and enhancing physical function of patients diagnosed with rheumatoid arthritis.

19.
Vet Microbiol ; 240: 108507, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31902511

RESUMO

In recent years, atypical infectious bursal disease (IBD) with severe immunosuppression has brought new threats to the poultry industry and has caused considerable economic losses. Novel variant infectious bursal disease virus (IBDV) has been identified as the etiological pathogen and for unknown reasons is widespread in poultry on many chicken farms in China that have been immunized with vaccines against very virulent IBDV (vvIBDV). Using immunoprotection experiments in specific-pathogen-free chickens, we first verified that novel variant IBDV could severely damage the bursa of Fabricius of the important immune organ of immunized chicken in the presence of antibodies induced by three types of vvIBDV vaccines, which is a primary reason for the current epidemic of atypical IBD. Monoclonal antibody reactivity patterns and cross-neutralization assays further confirmed the obvious antigenic mismatch between novel variant IBDV and vvIBDV. Sequence analysis of the genome of novel variant IBDV (SHG19 strain) was performed and the key amino acid residues that might be involved in antigenicity and virulence differences of novel variant IBDV compared to vvIBDV were further analyzed. This study not only determined the primary reason for the atypical IBD epidemic, but also remind us of the urgency for developing new vaccines against novel variant IBDV.

20.
Vet Microbiol ; 240: 108501, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31902513

RESUMO

Marek's disease (MD) is a highly contagious lymphoproliferative poultry disease caused by the oncogenic herpesvirus, Marek's disease virus (MDV). MDV strains have shown a continued evolution of virulence leading to immune failure, and MD cases continue to occur. Co-infection of virulent MDV strains is an important factor leading to viral evolution and host immune failure. This study conducted a laboratory diagnosis and analysis of a MDV infected flock. Testing showed that all samples were MDV positive. PCR detection identified a variable 132-base pair repeat (132-bpr) sequence copy number. This indicated that two virulent strains of MDV were co-infecting the flock. Therefore, we performed homology, sequence alignment, and phylogenetic tree analysis of MDV variant genes including meq, pp38, and RLORF4. Two MDV strains had co-infected the flock; one was the 132bpr two-copy characteristic strain (AH2C) and the other was a 132bpr three-copy characteristic strain (AH3C). Specific mutations in AH3C were found, suggesting that it is a new variant strain. Furthermore, the viral load of the two strains in vivo indicated that both strains had high and similar replication ability. There was no significant difference in the proportion of positive samples of the two strains causing disease. In the whole flock, neither strain displayed an obvious advantage. However, there was a dominant strain in individual chickens, with the exception of one sample. This study reported the co-infection regularity of two virulent MDV strains in the same flock, and even in the same chicken in field conditions. In the context of overall epidemiology, this study is a useful reference.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA