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1.
Small ; : e2000441, 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32243095

RESUMO

Efficient organic solar cells (OSCs) are fabricated using polymer PM6 as donor, and IPTBO-4Cl and MF1 as acceptors. The power conversion efficiency (PCE) of IPTBO-4Cl based and MF1 based binary OSCs individually arrive to 14.94% and 12.07%, exhibiting markedly different short circuit current density (JSC ) of 23.18 mA cm-2 versus 17.01 mA cm-2 , fill factor (FF) of 72.17% versus 78.18% and similar open circuit voltage (VOC ) of 0.893 V versus 0.908 V. The two acceptors, IPTBO-4Cl and MF1, have similar lowest unoccupied molecular orbital levels, which is beneficial for efficient electron transport in the ternary active layer. The PCE of optimized ternary OSCs arrives to 15.74% by incorporating 30 wt% MF1 in acceptors, resulting from the simultaneously increased JSC of 23.20 mA cm-2 , VOC of 0.897 V, and FF of 75.64% in comparison with IPTBO-4Cl based binary OSCs. The gradually increased FFs of ternary OSCs indicate the well-optimized phase separation and molecular arrangement with MF1 as morphology regulator. This work may provide a new viewpoint for selecting an appropriate third component to achieve efficient ternary OSCs from materials and photovoltaic parameters of two binary OSCs.

2.
Dalton Trans ; 2020 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-32239026

RESUMO

Treatment of iPr[NCN]Br (2,6-(2,6-iPr2C6H3C[double bond, length as m-dash]N)2C6H3Br) with nBuLi in THF and the subsequent addition of 1 equiv. of CoCl2, CoCl2(Ph3P)2, and CoBr2 gave pincer Co(ii) complexes {iPr[NCN]Co(µ-Cl)}2 (1d), iPr[NCN]CoClPh3P (1d-Ph3P), and iPr[NCN]CoBr2·Li(THF)4 (1d-LiBr) respectively in moderate yields, whereas the slow addition of in situ prepared iPr[NCN]Li to CoCl2 in THF afforded an unexpected mixed-valence cobalt(i/ii) complex κ2C,N,η6-iPr[NCN]Co-κN-CoCl3·Li(THF)4 (2d). Complex 2d was probably formed via a disproportionation reaction of the iPr[NCN]Co(ii) species with excess CoCl2 during the reaction. Nevertheless, addition of CoCl2 to in situ formed 1d-THF at room temperature did not lead to 2d but gave a trinuclear Co(ii) complex {iPr[NCN]Co(µ-Cl)(µ-Br/Cl)}2Co (1d-CoCl2) in moderate yield. Similar reactions using ligands containing small ortho groups in the imine moieties R[NCN]Br (2,6-(2,6-Me2C6H3C[double bond, length as m-dash]N)2C6H3Br, Me[NCN]Br; 2,6-(2,6-Et2C6H3C[double bond, length as m-dash]N)2C6H3Br, Et[NCN]Br; 2,6-(2,4,6-Me3C6H2C[double bond, length as m-dash]N)2C6H3Br, Mes[NCN]Br) and CoBr2, regardless of the reactant addition sequence, afforded mixed-valence cobalt(i/ii) complexes {κ2C,N,η6-R[NCN]Co-κN-CoBr(µ-Br)}2 (Me[NCN] (2a), Et[NCN] (2b), and Mes[NCN] (2c)), suggesting that the bulkiness of the ortho-groups in the imine moieties of the ligands plays an important role in the disproportionation reaction. In the presence of PMe3, Co(ii) complexes κ2C,N-R[NCN]CoBr(PMe3)2 (3a-d) and a bisligated cobalt(ii) complex κ3N,C,N-κ2C,N-iPr[NCN]2CoPMe3 (4d) can be prepared respectively in high yields. The molecular structures of 1d-LiBr, 1d-CoCl2, 2b, 2d, 3a, and 4d were confirmed by X-ray crystallographic analysis and the detailed mechanisms of the disproportionation reaction were proposed.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32252526

RESUMO

Two three-dimensional symmetric tetraphenylbutadiene derivatives decorated with diphenylamine or triphenylamine fragments are first prepared for use as hole-transporting materials (HTMs) in perovskite solar cells (PSCs). The HTMs are acquired using straightforward synthetic methods and facile purification techniques. The thermal stability, photophysical properties, electrochemical behaviors, computational study, hole mobility, X-ray diffraction, hole transfer dynamics, hydrophobicity, surface morphology, and photovoltaic performances of the HTMs are discussed. The highest power conversion efficiency (PCE) of CJ-04-based cell is 13.75%, which is increased to 20.06% when CJ-03 is used as HTM, superior to the PCE of the cell based on 2,2',7,7'-tetrakis(N,N-di-p-methoxyphenylamine)-9,9'-spirobifluorene (spiro-OMeTAD) (18.90%). The preparation cost of CJ-03 accounts for merely 23.1% of the price of commercial spiro-OMeTAD, while the concentration of CJ-03 solution used in the device fabrication (60.0 mg mL-1) is lower compared with that of the spiro-OMeTAD solution (72.3 mg mL-1). These results corroborate that the screw-like HTMs with a highly distorted configuration are facilely available and promising candidates for PSCs. More importantly, a practical solution is proposed to achieve moderate charge mobility and good film-formation ability of the HTMs simultaneously.

4.
Int Ophthalmol ; 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32253563

RESUMO

PURPOSE: To investigate the associations between single-nucleotide polymorphisms (SNPs) in the lysyl oxidase-like 1 (LOXL1) gene and copper chaperone genes and pseudoexfoliation-syndrome-related cataract (PEXC) in a Chinese Uygur population. METHODS: A case-control study was performed at the Second People's Hospital of Kashgar. Venous blood DNA was obtained from 70 patients with PEXC and 70 patients with age-related cataract (ARC). The exonic sequences of the LOXL1, antioxidant 1 copper chaperone (ATOX1), cytochrome C oxidase 17 copper chaperone (COX17), and copper chaperone for superoxide dismutase (CCS) genes were determined by Sanger sequencing, followed by a genetic association study. SIFT and PolyPhen-2 were used to predict the functional effects of the SNPs detected. The protein levels of CCS in lens-capsule specimens were measured by Western blotting. The plasma level of the CCS protein was measured using an enzyme-linked immunosorbent assay. RESULTS: Two coding SNPs (rs1048661 and rs3825942) in LOXL1 gene and a non-synonymous risk variant in CCS gene: CCS (c.717C>G, p.Asn239Lys) were significantly associated with PEXC. The TT genotype of rs1048661 was protective against PEXC in this Uygur population. The GG genotype of rs3825942 and its G allele were associated with an increased risk of PEXC. The CC genotype of c.717C>G and its C allele were protective against PEXC. The plasma level of CCS was significantly lower in patients with PEXC compared with those with ARC. CONCLUSIONS: The rs3825942 SNP of LOXL1 was strongly associated with PEXC in this Uygur population in China. CCS variants may represent a risk factor for PEXC. Our findings expand the understanding of the genetic base of PEXC.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32198687

RESUMO

Enhanced nutrient inputs due to human activities have been noted as a significant driving force for riverine nutrient exports which are responsible for the eutrophication issues in freshwaters. Current studies are mostly focused on the relationship between anthropogenic inputs and riverine exports, and little has been done to assess the role of nutrient mitigation measures in the fractional export of watershed nutrient inputs in urban regions. A highly urbanized watershed in Yun-Gui plateau of China, Lake Dianchi basin was studied as a case to assess the impact of nutrient mitigation measures on riverine nutrient exports. Based on net anthropogenic nitrogen and phosphorus inputs (NANI and NAPI, respectively) models, nutrient inputs from human activities in the basin from 1980 to 2015 were calculated, and the impact of nutrient mitigation measures were identified using a statistical model incorporating land use, precipitation, and temperature. Nutrient inputs from human action in the basin has increased rapidly, mainly from fertilizer application and food and feed imports. Enhanced riverine nutrient exports were found at the same time, and significantly correlated to nutrient inputs. The construction of water transfer projects and wastewater treatment plants in the basin has changed the controlling factors and processes of the fractional export of watershed nutrient inputs, which is weak in explanatory ability and eliminated the role of the land use. A modified model was established by incorporating the effect of water transfer projects and wastewater treatment plants, which showed a significant increase in model performance. The results from the modified model reveal that urban land percentage has become a positively driving force for the fractional export of watershed N and P inputs, and temperature a positive driving force for the fractional export of watershed N inputs while precipitation a negative driving force for the fractional export of watershed P inputs.

6.
Adv Mater ; : e1908498, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32130750

RESUMO

2D van der Waals heterostructures serve as a promising platform to exploit various physical phenomena in a diverse range of novel spintronic device applications. Efficient spin injection is the prerequisite for these devices. The recent discovery of magnetic 2D materials leads to the possibility of fully 2D van der Waals spintronics devices by implementing spin injection through the magnetic proximity effect (MPE). Here, the investigation of MPE in 2D graphene/CrBr3 van der Waals heterostructures is reported, which is probed by the Zeeman spin Hall effect through non-local measurements. Quantitative estimation of the Zeeman splitting field demonstrates a significant MPE field even in a low magnetic field. Furthermore, the observed anomalous longitudinal resistance changes at the Dirac point RXX,D with increasing magnetic field near ν = 0 may be attributed to the MPE-induced new ground state phases. This MPE revealed in the graphene/CrBr3 van der Waals heterostructures therefore provides a solid physics basis and key functionality for next-generation 2D spin logic and memory devices.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32209833

RESUMO

OBJECTIVE: To evaluate the efficacy of transcutaneous neuromuscular electrical stimulation (NMES) on swallowing disorders. DESIGN: MEDLINE/PubMed, Embase, CENTRAL, Web of science and PEDro were searched from their earliest record to 1 August 2019. All randomized controlled trials (RCT) and quasi-RCT were identified, which compared the efficacy of NMES plus traditional therapy (TT) versus TT in swallowing function. The GRADE approach was applied to evaluate the quality of evidence. RESULTS: Eight RCTs and three quasi-RCTs were included. These studies demonstrated a significant, moderate pooled effect size (SMD = 0.62; 95% CI = 0.06, 1.17). Studies stimulating suprahyoid muscle groups revealed a negative SMD of 0.17 (95% CI: -0.42, 0.08), whereas large effect size was observed in studies stimulating the infrahyoid muscle groups (SMD= 0.89; 95% CI: 0.47, 1.30) and stimulating the supra- and infrahyoid muscle groups (SMD= 1.4; 95% CI =1.07, 1.74). Stimulation lasting 45 minutes or less showed a large, significant pooled effect size (SMD= 0.89; 95% CI =0.58, 1.20). The quality of evidences was rated as low to very low. CONCLUSION: There is no firm evidence to conclude on the efficacy of NMES on swallowing disorders. Larger scale and well-designed RCTs are needed to reach robust conclusions.

8.
Eur J Pharmacol ; 876: 173052, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32135124

RESUMO

As diabetic macroangiopathy is becoming increasingly prevalent, it is urgent to explore preventive and therapeutic drugs and study the mechanism. Diabetic mice were induced by intraperitoneal injection of streptozotocin (STZ)for five consecutive days. Diabetic mice were divided into diabetic and allicin groups. After sacrifice, frozen aortic root sections were immunohistochemically stained for nuclear factor erythroid 2-related factor 2 (Nrf2) and inflammation cytokine-tumor necrosis factor α (TNF-α), and the remaining aortic tissues were analyzed by Western blot for the expression of proinflammation genes. In vitro, Nrf2 and inflammatory relative protein expression levels in Human Umbilical Vein Endothelial Cells (HUVECs) were examined. HUVECs proliferation and apoptosis were measured. TNF-α expression was increased in diabetic group compared to that in control group; this effect was alleviated in allicin-treated mice. Inflammation relative protein expression of Vascular Cell Adhesion Molecule 1(VCAM-1), Matrix metalloproteinase 2 (MMP-2), Inducible Nitric Oxide Synthase (iNOS), and monocyte chemotactic protein 1 (MCP-1) was higher in the diabetic group than in the control group; however, allicin treatment inhibited these diabetes-induced increase. In vitro, allicin treatment reversed the hyperglycemia-induced reduction in proliferation, and decreased the apoptosis induced by high glucose. Inflammation relative protein expression was consistent with that in vivo. Additionally, the expression of nuclear factor kappa-B (NF-κB)and Nrf2 was increased in both DM mice and HUVECs; allicin treatment induced a significant reduction in NF-κB level and improvement in Nrf2 level. Allicin alleviates inflammation caused by diabetic macroangiopathy, and the mechanism may occur via increasing Nrf2 and decreasing NF-κB.

9.
J Asian Nat Prod Res ; : 1-10, 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32138546

RESUMO

Steroidal saponins named polyphyllin are the major active components of Paris polyphylla. Cycloartenol synthase (CAS) is a key enzyme that catalyzes the formation of the sterol scaffold. In this study, we cloned a putative CAS gene from Paris polyphylla. Heterologous expression in yeast indicated that PpCAS can convert 2,3-oxidosqualene into cycloartenol. qRT-PCR analysis showed that the expression of PpCAS was highest in leaves and lowest in roots. To our best knowledge, this is the first report of the functional characterization of cycloartenol synthase from Paris polyphylla, which lays the foundation for further analysis of the biosynthesis pathway of polyphyllins.

10.
Immunopharmacol Immunotoxicol ; 42(2): 138-146, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32116062

RESUMO

Objectives: Paraquat (PQ) poisoning can induce mitophagy and pulmonary fibrosis. Cyclosporine A (CsA) is an inhibitor of mitophagy. This study aimed at investigating whether CsA could inhibit PQ-induced mitophagy and pulmonary fibrosis in rats.Materials and Methods: Male Sprague-Dawley (SD) rats were treated with vehicle saline (control), 50 mg/kg PQ by gavage alone, or together with different doses of CsA. At 14 days post-induction, the levels of pulmonary fibrosis and PTEN-induced putative kinase 1 (PINK1) and Parkin expression in individual rats and mitochondrial membrane potential (MMP) in lung cells were measured. Moreover, A549 cells were treated with PQ or PQ + CsA for 24 h and the levels of PINK1, Parkin, fibronectin, collagen I and LC3 I and II expression and MMP were examined. Finally, the impact of PINK1 overexpression on the PQ or PQ + CsA-modulated fibronectin and collagen I expression in A549 cells was tested.Results: PQ exposure significantly increased the levels of hydroxyproline and collagen I expression and collagen fiber accumulation in the lung of rats, which were mitigated by CsA treatment. Furthermore, treatment with CsA significantly improved the PQ-decreased MMP and abrogated PQ-upregulated PINK1 and Parkin expression in the lungs of rats. In addition, CsA treatment decreased the PQ-induced fibrosis and mitophagy and PQ-impaired MMP as well as PQ-upregulated PINK1 and Parkin expression in A549 cells. The later effect of CsA was abrogated by PINK1 overexpression in A549 cells.Conclusions: Therefore, CsA can inhibit the PQ-induced mitophagy and pulmonary fibrosis by attenuating the PINK1/Parkin signaling.

11.
Cancer Res ; 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32156780

RESUMO

Dysregulation of Wnt/ß-catenin signaling is frequently observed in human gastric cancer. Elucidation of the tumor immune microenvironment is essential for understanding tumorigenesis and for the development of immunotherapeutic strategies. However, it remains unclear how ß-catenin signaling regulates the tumor immune microenvironment in the stomach. Here we identify CCL28 as a direct transcriptional target gene of ß-catenin/T cell factor (TCF). Protein levels of ß-catenin and CCL28 positively correlated in human gastric adenocarcinoma. Activation of CCL28 by ß-catenin recruited Regulatory T (Treg) cells in a transwell migration assay. In a clinically relevant mouse gastric cancer model established by Helicobacter (H.) felis infection and MNU treatment, inhibition of ß-catenin/TCF activity by a pharmacological inhibitor iCRT14 suppressed CCL28 expression and Treg cell infiltration in the stomach. Moreover, an anti-CCL28 antibody attenuated Treg cell infiltration and tumor progression in H. felis/MNU mouse models. Diphtheria toxin (DT)-induced Treg cell ablation restrained gastric cancer progression in H. felis/MNU-treated DEREG (Foxp3-DTR) mice, clarifying the tumor-promoting role of Treg cells. Thus, the ß-catenin-CCL28-Treg cell axis may serve as an important mechanism for immunosuppression of the stomach tumor microenvironment. Our findings reveal an immunoregulatory role of ß-catenin signaling in stomach tumors and highlight the therapeutic potential of CCL28 blockade for the treatment of gastric cancer.

12.
Life Sci ; 250: 117551, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32179075

RESUMO

AIMS: Increasing evidence indicates that FK866, a specific noncompetitive nicotinamide phosphoribosyl transferase inhibitor, exhibits a protective effect on acute lung injury (ALI). Autophagy plays a pivotal role in sepsis-induced ALI. However, the contribution of autophagy and the underlying mechanism by which FK866-confered lung protection remains elusive. Herein, we aimed to study whether FK866 could alleviate sepsis-induced ALI via the JNK-dependent autophagy. MAIN METHODS: Male C57BL/6 mice were subjected to cecal ligation and puncture (CLP) to establish the polymicrobial sepsis mice model, and treated with FK866 (10 mg/kg) at 24, 12 and 0.5 h before the CLP procedure. The lung protective effects were measured by lung histopathology, tissue edema, vascular leakage, inflammation infiltration, autophagy-related protein expression and JNK activity. A549 cells were stimulated with LPS (1000 ng/ml) to generate the ALI cell model, and pretreated with FK866 or SP600125 for 30 min to measure the autophagy-related protein expression and JNK activity. KEY FINDINGS: Our results demonstrated that FK866 reduced lung injury score, tissue edema, vascular leakage, and inflammatory infiltration, and upregulated autophagy. The protective effect of autophagy conferred by FK866 on ALI was further clarified by using 3-methyladenine (3MA) and rapamycin. Additionally, the activity of JNK was suppressed by FK866, and inhibition of JNK promoted autophagy and showed a benefit effect. SIGNIFICANCE: Our study indicates that FK866 protects against sepsis-induced ALI by induction of JNK-dependent autophagy. This may provide new insights into the functional mechanism of NAMPT inhibition in sepsis-induced ALI.

13.
J Virol ; 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32213616

RESUMO

Different from other subgroups of avian leukosis viruses (ALVs), ALV-J is highly pathogenic. It is the main culprit causing myeloid leukemia and hemangioma in chickens. The specialty of env gene of ALV-J with low homology to those of other ALVs is linked to its unique pathogenesis, but the underlying mechanism remains unclear. Previous studies show the env of ALV-J can be grouped into three species based on the tyrosine motifs in the cytoplasmic domain (CTD) of Gp37, i.e., the inhibitory, bi-functional and active groups. To explore whether C-terminus or the tyrosine motifs in the CTD of Gp37 affect the pathogenicity of ALV-J, a set of ALV-J infectious clones containing different C-termini of Gp37 or the mutants at the tyrosine sites were tested in vitro and in vivo Viral growth kinetics did not only indicate ALV-J with active env is the fastest in replication and ALV-J with inhibitory env is the lowest, but also indicate the tyrosine sites essentially affected the replication of ALV-J. Moreover, in vivo studies demonstrated the chickens infected by ALV-J with active or bi-functional env showed higher viremia, cloacal viral shedding and viral tissue load compared with those infected by ALV-J with inhibitory env Notably, the chickens infected by ALV-J with active or bi-functional env showed significant loss of body weight compared with the control chickens. Taken together, these findings reveal the C-terminus of Gp37 plays a vital role in ALV-J pathogenesis, and change from inhibitory env to bi-functional or active env increases the pathogenesis of ALV-J.Importance: ALV-J can cause severe immunosuppression and myeloid leukemia in the infected chickens. However, no vaccine or antiviral drug is available against ALV-J, and the mechanism for ALV-J pathogenesis needs to be elucidated. It is generally believed that the gp85 and LTR of ALV contribute to its pathogenesis. Here, we found C-terminus and the tyrosine motifs (YxxM, ITIM, and ITAM-like) in the CTD of Gp37 of ALV-J could affect the pathogenicity of ALV-J in vitro and in vivo The pathogenicity of ALV-J with Gp37 containing ITIM only was significant weaker than ALV-J with Gp37 containing both YxxM and ITIM and ALV-J with Gp37 containing both YxxM and ITAM-like. This study highlights the vital role of the C-terminus of Gp37 in the pathogenesis of ALV-J, and thus provides a new perspective to elucidate the interaction between ALV-J and host and a molecular basis to develop efficient strategies against ALV-J.

14.
J Mater Chem B ; 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32219269

RESUMO

The scheduled delivery of synergistic drug combinations is increasingly recognized as highly effective against advanced solid tumors. Of particular interest are composite systems that release a sequence of drugs with defined kinetics and molar ratios to enhance therapeutic effect, while minimizing the dose to patients. In this work, we developed a homogeneous composite comprising modified graphene oxide (GO) nanoparticles embedded in a Max8 peptide hydrogel, which provides controlled kinetics and molar ratios of release of doxorubicin (DOX) and gemcitabine (GEM). First, modified GO nanoparticles (tGO) were designed to afford high DOX loading and sustained release (18.9% over 72 h and 31.4% over 4 weeks). Molecular dynamics simulations were utilized to model the mechanism of DOX loading as a function of surface modification. In parallel, a Max8 hydrogel was developed to release GEM with faster kinetics and achieve a 10-fold molar ratio to DOX. The selected DOX/tGO nanoparticles were suspended in a GEM/Max8 hydrogel matrix, and the resulting composite was tested against a triple negative breast cancer cell line, MDA-MB-231. Notably, the composite formulation afforded a combination index of 0.093 ± 0.001, indicating a much stronger synergism compared to the DOX-GEM combination co-administered in solution (CI = 0.396 ± 0.034).

15.
iScience ; 23(4): 100975, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32222698

RESUMO

Actin stress fibers guide cell migration and morphogenesis. During centripetal flow, actin transverse arcs fuse accompanied by the formation of myosin II stacks to generate mechanosensitive actomyosin bundles. However, whether myosin II stack formation plays a role in cell mechano-sensing has remained elusive. Myosin-18B is a "glue" molecule for assembling myosin II stacks. By examining actin networks and traction forces, we find that cells abolishing myosin-18B resemble Ca2+∕calmodulin-dependent kinase kinase 2 (CaMKK2)-defective cells. Inhibition of CaMKK2 activity reverses the strong actin network to thin filaments in myosin-18B-overexpressing cells. Moreover, AMP-activated protein kinase (AMPK) activation is able to relieve the thin stress fibers by myosin-18B knockout. Importantly, lack of myosin-18B compromises AMPK-vasodilator-stimulated phosphoprotein and RhoA-myosin signaling, thereby leading to defective persistent migration, which can be rescued only by full-length and C-extension-less myosin-18B. Together, these results reveal a critical role of myosin-18B in the mechanosensitive regulation of migrating cells.

16.
Nanotechnology ; 31(22): 225701, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32167934

RESUMO

In this work, we reported the tailored design of highly efficient Fe3O4-Au magnetic nanocomposite (MNP) catalysts. Fe3O4 nanocrystals with three different morphologies have been developed with engineered amounts of urea, and the plausible mechanism has been proposed. Then by controlling the amount of Au seeds, Fe3O4-Au MNPs with different morphologies and tunable Au deposition have been realized. Characterizations including x-ray diffraction (XRD), transmission electron microscopy (TEM), Mössbauer spectra, and elemental mapping are implemented to unveil the structural and physical characteristics of the successfully developed Fe3O4-Au MNPs with different morphologies. The catalytic ability of Fe3O4-Au MNPs with different morphologies have been compared by applying them to degrading RhB and 4-NP, meanwhile the correlation between the amount of Au seeds and the turnover frequency as well as the catalytic ability of Fe3O4-Au MNPs is investigated systematically. We found that the flower-like Fe3O4-Au MNPs with 20 ml Au seeds added achieved the best degradation efficiency of 96.7%, and their catalytic ability were almost unchanged after recycling. Out study sheds the light into the tailored design of highly efficient and recyclable catalysts for RhB and 4-NP.

17.
Sensors (Basel) ; 20(6)2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32168872

RESUMO

We designed an end-to-end dual-branch residual network architecture that inputs a low-resolution (LR) depth map and a corresponding high-resolution (HR) color image separately into the two branches, and outputs an HR depth map through a multi-scale, channel-wise feature extraction, interaction, and upsampling. Each branch of this network contains several residual levels at different scales, and each level comprises multiple residual groups composed of several residual blocks. A short-skip connection in every residual block and a long-skip connection in each residual group or level allow for low-frequency information to be bypassed while the main network focuses on learning high-frequency information. High-frequency information learned by each residual block in the color image branch is input into the corresponding residual block in the depth map branch, and this kind of channel-wise feature supplement and fusion can not only help the depth map branch to alleviate blur in details like edges, but also introduce some depth artifacts to feature maps. To avoid the above introduced artifacts, the channel interaction fuses the feature maps using weights referring to the channel attention mechanism. The parallel multi-scale network architecture with channel interaction for feature guidance is the main contribution of our work and experiments show that our proposed method had a better performance in terms of accuracy compared with other methods.

18.
J Pediatr Surg ; 2020 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-32171534

RESUMO

BACKGROUND: To investigate the safety of using ABO incompatible (ABO-i) liver grafts in pediatric patients under our prophylactic strategies. METHODS: A total number of 544 pediatric liver transplantations between January 2013 and December 2017 performed in Organ Transplant Center, Tianjin First Central Hospital were included in this study. The recipients were divided into 3 groups based on the compatibility of donor-recipient blood type matching (ABO-identical group, n = 352, ABO-compatible group, n = 121 and ABO-incompatible group, n = 71). Recipient characteristics, perioperative data, postoperative complications and recipient survival rate were compared. The recipient outcomes between living-related and non-living-related ABO-incompatible liver graft recipients were also compared. RESULTS: The median follow-up time in three groups were 3.4 (1.8, 6.4) years, 3.2 (1.8, 6.1) years and 2.8 (1.8, 6.2) years, without statistical difference. The cumulative 1-year and 3-year graft survival rate were 94.3% and 94.0% in ABO-id group, 93.1% and 93.1% in ABO-c group and 97.1% and 97.1% in ABO-i group. The cumulative 1-year and 3-year recipient survival rate were 96.1% and 95.5% in ABO-id group, 94.8% and 94.8% in ABO-c group and 97.1% and 97.1% in ABO-i group, respectively. No significant difference was seen among three groups. The recipient characteristics and perioperative data were similar among three groups. The recipients in ABO-i group showed significantly lower incidence of portal vein stenosis. Apart from that, three groups shared equal incidence of other surgical complications and acute rejection. Among ABO-i liver graft recipients, the cumulative 1-year and 3-year recipient survival rate were 98.2% and 98.2% in living donor liver transplant (LDLT) recipients and 92.9% and 92.9% in deceased donor liver transplant (DDLT) recipients, without significant difference. The incidence of hepatic artery thrombosis was significantly higher in DDLT group compared with LDLT group, while the other complications were similar between two groups. CONCLUSION: Our data revealed that the application of ABO-i liver grafts in pediatric liver transplantation under rational peri-operative management strategy is a safe measure to increase donor availability for pediatric patients in Chinese population. LEVELS OF EVIDENCE: III.

19.
Stem Cells Dev ; 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32178572

RESUMO

Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer that is unresponsive to chemotherapy; therefore, understanding the causes of chemotherapy resistance is important. The cancer stem cell theory postulates that cancer stem cells (CSCs) are the source of tumor chemoresistance. We enrich laryngeal CSCs to overcome chemoresistance of LSCC. A laryngeal cancer xenograft model was established, and a low dose of cisplatin was administered until chemoresistance arose. A next-generation xenograft model was established using surviving tumor cells, and the test was repeated 4 times to screen for CSCs. Cell-function experiments were performed on each tumor cell generation (m1, m2, m3, and m4). The m3 line, with the highest stemness, was selected for transcriptome sequencing. LY6D was selected for clinical sample validation and functional verification. LY6D expression was detected in 107 laryngeal cancer samples, with high expression in 91 of these samples. LY6D expression was correlated with pathological T- and clinical stages, and with cervical lymph node metastasis. The siLY6D group exhibited reduced adhesion and chemoresistance to cisplatin, 5-fluorouracil, and paclitaxel. LY6D is upregulated in laryngeal cancer and may serve as a biomarker for chemoresistance in CSCs. Moreover, LY6D could serve as an alternative antigenic peptide in the targeted treatment of laryngeal cancer.

20.
Plant Physiol Biochem ; 150: 56-70, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32114400

RESUMO

Protein acetylation (KAC) is a significant post-translational modification, which plays an essential role in the regulation of growth and development. Unfortunately, related studies are inadequately available in angiosperms, and to date, there is no report providing insight on the role of protein acetylation in cotton fiber development. Therefore, we first compared the lysine-acetylation proteome (acetylome) of upland cotton ovules in the early fiber development stages by using wild-type as well as its fuzzless-lintless mutant to identify the role of KAC in the fiber development. A total of 1696 proteins with 2754 acetylation sites identified with the different levels of acetylation belonging to separate subcellular compartments suggesting a large number of proteins differentially acetylated in two cotton cultivars. About 80% of the sites were predicted to localize in the cytoplasm, chloroplast, and mitochondria. Seventeen significantly enriched acetylation motifs were identified. Serine and threonine and cysteine located downstream and upstream to KAC sites. KEGG pathway enrichment analysis indicated oxidative phosphorylation, fatty acid, ribosome and protein, and folate biosynthesis pathways enriched significantly. To our knowledge, this is the first report of comparative acetylome analysis to compare the wild-type as well as its fuzzless-lintless mutant acetylome data to identify the differentially acetylated proteins, which may play a significant role in cotton fiber development.

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