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1.
Cell Death Discov ; 8(1): 393, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36127329

RESUMO

The mechanisms by which prostate cancer (PCa) progresses to the aggressive castration-resistant stage remain uncertain. Zinc finger of the cerebellum 5 (ZIC5), a transcription factor belonging to the ZIC family, is involved in the pathology of various cancers. However, the potential effect of ZIC5 on PCa malignant progression has not been fully defined. Here, we show that ZIC5 is upregulated in PCa, particularly in metastatic lesions, in positive association with poor prognosis. Genetic inhibition of ZIC5 in PCa cells obviously attenuated invasion and metastasis and blunted the oncogenic properties of colony formation. Mechanistically, ZIC5 functioned as a transcription factor to promote TWIST1-mediated EMT progression or as a cofactor to strengthen the ß-catenin-TCF4 association and stimulate Wnt/ß-catenin signaling. Importantly, ZIC5 and the androgen receptor (AR) form a positive feed-forward loop to mutually stimulate each other's expression. AR, in cooperation with its steroid receptor coactivator 3 (SRC-3), increased ZIC5 expression through binding to the miR-27b-3p promoter and repressing miR-27b-3p transcription. In turn, ZIC5 potentiated AR, AR-V7, and AR targets' expression. Besides, ZIC5 inhibition reduced AR and AR-V7 protein expression and enhanced the sensitivity of PCa to enzalutamide (Enz) treatment, both in vitro and in vivo. These findings indicate that the reciprocal activation between AR and ZIC5 promotes metastasis and Enz resistance of PCa and suggest the therapeutic value of cotargeting ZIC5 and AR for the treatment of advanced PCa.

2.
Front Immunol ; 13: 882919, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36131922

RESUMO

Given that vaccine-induced adverse effects were mostly based on previous laboratory research and clinical trials, real-world data on the safety of coronavirus disease 2019 (COVID-19) vaccination were lacking. This study reported the adverse events (AEs) among inactivated COVID-19 vaccine recipients. Data were collected from a total of 2,808 hospital employees and their family members in Wuhan, China, with all of them receiving the first dose of inactivated COVID-19 vaccines from two pharmaceutical companies. The first dose was given between 29th April and 13th May 2021. A total of 2,732 vaccinees received the second dose between 27th May and 8th July 2021. The whole process of receiving the vaccine was monitored by clinical pharmacists, and the information on AEs including demographics, occurrence, types, and severity was recorded through an online questionnaire and telephone follow-up. Most of the common AEs were mild and tolerable, and the overall incidence of AEs was lower than the data from the safety profile in clinical trials. Moreover, the incidence of AEs in the first dose (21.30%, 598) was higher than that in the second dose (16.07%, 439). Furthermore, the first injection had more severe AEs (4, 0.14%) than the second injection (2, 0.07%). The AEs involved the skin, muscle, respiratory tract, gastrointestinal tract, cardiovascular system, and other tissues and systems. The most common AE was pain at the injection site (first dose: 10.19%, second dose: 12.55%). All the vaccinees with AEs for both doses recovered fully in the end. It was noted that some AEs might cause blood coagulation disorder and bleeding risk. Therefore, ongoing monitoring of AEs after COVID-19 vaccination is essential in evaluating the benefits and risks of each vaccine.


Assuntos
COVID-19 , Vacinas , COVID-19/prevenção & controle , Vacinas contra COVID-19/efeitos adversos , Humanos , Preparações Farmacêuticas , Farmacêuticos
3.
Nanoscale Adv ; 4(13): 2884-2892, 2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-36131998

RESUMO

Anti-/de-icing of glass surfaces is of great importance in present daily life. The long-standing challenge in this field is largely due to the lack of stable multifunctional coatings that can be conveniently and economically constructed on the glass surface, and more importantly, are capable of retaining the original transparency of glass ranging from the visible to the near infrared spectrum. Herein, a direct spraying sol method on the glass surface to prepare a highly transparent and photothermal composite coating is reported. Such multifunctional coating of Cu7S4 nanoparticles/organo-silicone sols has displayed a good photothermal conversion property and hydrophobic property and therefore yields excellent anti-icing and self-melting ice properties. The condensation time of water droplets can be extended to 86 s even at -10 °C, which is 3.42 times delayed relative to ordinary blank glass. And the adhesion strength of ice is largely reduced to 72 KPa, which is as low as ∼1/3 that of ordinary glass. Meanwhile, the subcooling of adhering droplets is reduced to -12 °C under one solar illumination condition and exhibits a rapid de-icing capability. More impressively, the prepared functional coating glass shows an outstanding transmittance of more than 75% in the visible region, while it is over the minimum glass transmittance limit allowed by Safety Standards for Glass (GB9656-2016, China). In addition, the multifunctional photothermal glass coating exhibits good physical/chemical stability, which facilitates the long-term application of the coating in different environments.

4.
Int J Biol Macromol ; 221: 1394-1403, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36116597

RESUMO

The Ascaridoidea family and Heterakoidea family are the most common and typical representative of large parasites. Although our understanding of these parasites' diversity has expanded by analyses of some mitochondrial genes, there is limited information on these species' evolutionary rates. Here we determined ten complete mitogenome sequences of five subfamilies of Ascaridoidea and one subfamily of Heterakoidea. The phylogenetic tree divided the Ascaridoidea into six monophyletic major clades, and the divergence time of Heterakoidea family and Ascaridoidea family can be placed during the early Carboniferous Period (300-360 Mya). The reconstruction of the ancestral state showed that the gene orders of all species in Ascaridoidea were conserved, and the Heterakoidea had obvious genome rearrangement. The conserved blocks between them were divided into five and the main types are tandem-duplication/random loss (TDRL). These results will help to better understand the gene rearrangements and evolutionary position of ascaris species.

5.
Oral Dis ; 2022 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-36152024

RESUMO

OBJECTIVE: To use quantitative MRI to assess gender differences in lateral pterygoid muscle (LPM) characteristics in patients with anterior disk displacement (ADD). METHODS: LPM of 51 patients diagnosed with temporomandibular joint disorders (TMD) who underwent T1-weighted Dixon and T1-mapping sequences were retrospectively analyzed. There were 34 female patients (10 with bilateral normal position disk [NP]; 24 with bilateral ADD) and 17 male patients (8 with bilateral NP; 9 with bilateral ADD) among them. After controlling for age, differences in fat fraction, T1 value, volume and histogram features related to gender and disk status were tested with 2-way ANCOVA or Quade ANCOVA with Bonferroni correction. RESULTS: Volume of LPM in NP was significantly smaller than that of ADD (p < .001). Fat fraction of LPM in females with NP was significantly higher than males with NP (p < .05). Females with ADD showed a significantly higher T1 value (p < .05), and higher intramuscular heterogeneity than males with ADD. CONCLUSIONS: LPM in female TMD patients presented more fatty infiltration in the NP stage and might present more fibrosis in the ADD stage compared with males. Together, this leads to more serious intramuscular heterogeneity during the pathogenesis of ADD in females.

6.
Animals (Basel) ; 12(18)2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36139290

RESUMO

N acetylcysteine (NAC) affects antioxidation and reactive oxygen species scavenging in the body and thereby promotes embryonic development and implantation and inhibits inflammation. The mechanism through which NAC regulates reproductive performance in the uteri of goats during early gestation remains unclear. In this study, the treatment group was fed 0.07% NAC for the first 35 days of gestation, whereas the control group received no NAC supplementation. The regulatory genes and key pathways associated with goat reproductive performance under NAC supplementation were identified by RNA-seq. RT-qPCR was used to verify the sequencing results and subsequently construct tissue expression profiles of the relevant genes. RNA-seq identified 19,796 genes coexpressed in the control and treatment groups and 1318 differentially expressed genes (DEGs), including 787 and 531 DEGs enriched in the treatment and control groups, respectively. A GO analysis revealed that the identified genes mapped to pathways such as cell activation, cytokine production, cell mitotic processes, and angiogenesis, and a KEGG enrichment analysis showed that the DEGs were enriched in pathways associated with reproductive regulation, immune regulation, resistance to oxidative stress, and cell adhesion. The RT-qPCR analysis showed that BDNF and CSF-1 were most highly expressed in the uterus, that WIF1 and ESR2 showed low expression in the uterus, and that CTSS, PTX3, and TGFß-3 were most highly expressed in the oviduct, which indicated that these genes may be directly or indirectly involved in the modulation of reproduction in early-gestation goats. These findings provide fundamental data for the NAC-mediated modulation of the reproductive performance of goats during early gestation.

7.
Genes (Basel) ; 13(9)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36140694

RESUMO

(1) Background: RNA binding motif 20 (RBM20) regulates mRNA splicing specifically in muscle tissues. Missense mutations in the arginine/serine (RS) domain of RBM20 lead to abnormal gene splicing and have been linked to severe dilated cardiomyopathy (DCM) in human patients and animal models. Interestingly, many of the reported DCM-linked missense mutations in RBM20 are in a highly conserved RSRSP stretch within the RS domain. Recently, it was found that the two Ser residues within this stretch are constitutively phosphorylated, yet the identity of the kinase(s) responsible for phosphorylating these residues, as well as the function of RSRSP phosphorylation, remains unknown. (2) Methods: The ability of three known SR protein kinases (SRPK1, CLK1, and AKT2) to phosphorylate the RBM20 RSRSP stretch and regulate target gene splicing was evaluated by using both in vitro and in vivo approaches. (3) Results: We found that all three kinases phosphorylated S638 and S640 in the RSRSP stretch and regulated RBM20 target gene splicing. While SRPK1 and CLK1 were both capable of directly phosphorylating the RS domain in RBM20, whether AKT2-mediated control of the RS domain phosphorylation is direct or indirect could not be determined. (4) Conclusions: Our results indicate that SR protein kinases regulate the splicing of a cardiomyopathy-relevant gene by modulating phosphorylation of the RSRSP stretch in RBM20. These findings suggest that SR protein kinases may be potential targets for the treatment of RBM20 cardiomyopathy.

8.
Artigo em Inglês | MEDLINE | ID: mdl-36121292

RESUMO

Osteoarthritis (OA) is the main joint disease associated with aging. Previous studies have confirmed that both osteopontin (OPN) and αvß3 integrin are involved in the progression of knee OA. The purpose of this study was to determine the expression of OPN and αvß3 integrin and chondrocyte senescence levels in OA. Forty-six cartilage tissues from normal and knee OA patients were divided into 4 groups of normal, minor, moderate, and severe lesions based on the Mankin score. Immunohistochemistry and western blotting were used to determine the expression of αvß3, OPN, and senescent-associated-ß-galactosidase (SAß-gal) in articular cartilage. Then, Spearman's correlation was used to analyze the correlations between the Mankin scores and αvß3, OPN and SAß-gal. Pearson correlation analysis was used to analyze the correlations among αvß3, OPN, and SAß-gal. The expression of OPN, αvß3, and SAß-gal in articular cartilage was explored. αvß3, OPN, and SAß-gal proteins were all elevated in OA cartilage, and the correlation coefficient between the Mankin score and the average optical density value of αvß3, OPN, SAß-gal were r=0.60, r=0.75, and r=0.87, respectively, all P<0.001; the correlation between the average optical density value of αvß3 and OPN was r=0.3191, P<0.05; the correlation between αvß3 and SAß-gal was r=0.4955, P<0.001; and the correlation between OPN and SAß-gal was r=0.7821, P<0.001. The correlations among αvß3, OPN, and SAß-gal expression in articular cartilage might be important in OA progression and pathogenesis. Nonetheless, more research is needed to elucidate the exact contribution of αvß3, OPN, and SAß-gal to the degenerative process of OA.

9.
Artigo em Inglês | MEDLINE | ID: mdl-36121689

RESUMO

In order to get well understanding of the production trend of plastic waste in Chengdu, as well as its compositions and environmental impacts, based on the statistical data from 2005 to 2019, the back propagation (BP) neural network model was used to predict the production amount of plastic waste in Chengdu. Compositions of plastic waste were analyzed by sampling. In addition, inhale particles and greenhouse gas emissions (GHGs) from plastic waste incineration, bisphenol A (BPA) from plastic waste landfill were evaluated. Results indicated that, (1) economic development, urban construction level, residents' consumption ability and plastic waste production were positively correlated to different degrees. (2) The production of plastic waste in Chengdu in 2025 and 2030 will reach 865.3 and 931 kiloton (Kt) respectively. (3) High density polyethylene (HDPE) and polypropylene (PP) are the two main components of plastic waste in Chengdu, accounted for 40.17% and 24.96%, respectively. (4) Different degrees of environmental impacts occurred during plastic waste incineration and landfill, taking the year of 2019 as an example, the incineration of plastic waste in Chengdu produced 2874.82-4711.73 tons of inhalable particulate matter (PM) and emitted 725.4-867.4 Kt of CO2, about 65.02-910.27 kg of bisphenol A (BPA) leached from sanitary landfill. (5) positive policies and measures from the beginning to the end-of-life of plastics should be carried out in the future, which will improve plastic waste management level in Chengdu, and mitigate the side-impacts from plastic waste treatment and disposal.

10.
Front Oncol ; 12: 965255, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119475

RESUMO

Background: The aim of this study was to investigate whether circumferential resection margin (CRM) status has an impact on survival and recurrence in esophageal squamous cell carcinoma after neoadjuvant chemoradiotherapy. Methods: We screened patients with esophageal squamous cell carcinoma who underwent esophagectomy from January 2017 to December 2019. The CRM was reassessed. Patients were grouped into a CRM of 1 mm or less (0 < CRM ≤ 1 mm) and a CRM greater than 1 mm (CRM>1 mm). The impact of CRM on survival was investigated using Kaplan-Meier analysis and Cox regression modeling. The optimal CRM cut point was evaluated using restricted cubic spline curve. Results: A total of 89 patients were enrolled in this study. The CRM status was an independent risk factor for the prognosis (HR: 0.35, 95% CI: 0.16-0.73). Compared with a CRM of 1 mm or less, a CRM greater than 1 mm had better overall survival (HR: 0.35, 95% CI: 0.16-0.73, log-rank P = 0.011), longer disease-free survival (HR: 0.51, 95% CI: 0.27-0.95, log-rank P = 0.040), and less recurrence (HR: 0.44, 95% CI: 0.23-0.85, log-rank P = 0.015). We visualized the association between CRM and the hazard ratio of survival and identified the optimal cut point at 1 mm. Conclusions: A CRM greater than 1 mm had better survival and less recurrence compared to a CRM of 1 mm or less. A more radical resection with adequate CRM could benefit survival in patients with esophageal squamous cell carcinoma after neoadjuvant therapy.

11.
Am J Cancer Res ; 12(8): 3857-3869, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119824

RESUMO

Follicular lymphoma (FL) has a high degree of heterogeneity both clinically and molecularly. Early treatment failure (ETF), progression or relapse within 24 months of frontline immunochemotherapy is associated with a poor prognosis in FL. However, the clinical utility of ETF at diagnosis is limited. The maximum standardized uptake value (SUVmax) is a metabolic parameter for positron emission tomography/computed tomography (PET/CT); nevertheless, the relationship between SUVmax and ETF remains unclear. Thus, identifying early biomarkers that incorporate SUVmax and other clinical correlative variables could be helpful in identifying patients at high risk of ETF. A nomogram consisted of three independent variables, including SUVmax ≥ 12, beta-2 microglobulin > 3 mg/L, and Ki67 > 40%, was established to predict ETF in 127 patients with grade 1, 2, or 3a FL from the First Hospital of Jilin University (training cohort) and was validated using data from the Duke University Medical Center (validation cohort, n=95). The nomogram demonstrated prognostic accuracy in predicting ETF (sensitivity 70.8% and specificity 83.5% in the training cohort; sensitivity 84.2% and specificity 68.4% in the validation cohort). The patients were stratified into three groups: low-, intermediate-, and high-risk. In the training cohort, the corresponding 5-year progression-free survival (PFS) rates were 81.7%, 73.4%, and 34.9%, and the 5-year overall survival (OS) rates were 97.4%, 87.4%, and 62.3%, respectively. In the validation cohort, the 5-year PFS rates were 77.7%, 52.9%, and 34.8%, and the 5-year OS rates were 96.4%, 94.1%, and 73.7%, respectively. This was the first study to use a nomogram with SUVmax to predict ETF in FL to identify a subset of patients who might benefit from individualized targeted therapy.

12.
Methods ; 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36122881

RESUMO

Abnormal co-occurrence medical visit behavior is a form of medical insurance fraud. Specifically, an organized gang of fraudsters hold multiple medical insurance cards and purchase similar drugs frequently at the same time and the same location in order to siphon off medical insurance funds. Conventional identification methods to identify such behaviors rely mainly on manual auditing, making it difficult to satisfy the needs of identifying the small number of fraudulent behaviors in the large-scale medical data. On the other hand, the existing single-view bi-clustering algorithms only consider the features of the time-location dimension while neglecting the similarities in prescriptions and neglecting the fact that fraudsters may belong to multiple gangs. Therefore, in this paper, we present a multi-view bi-clustering method for identifying abnormal co-occurrence medical visit behavioral patterns, which performs cluster analysis simultaneously on the large-scale, complex and diverse visiting record dimension and prescription dimension to identify bi-clusters with similar time-location features. The proposed method constructs a matrix view of patients and visit records as well as a matrix view of patients and prescriptions, while decomposing multiple data matrices into sparse row and column vectors to obtain a consistent patient population across views. Subsequently the proposed method identifies the corresponding abnormal co-occurrence medical visit behavior and may greatly facilitate the safe operations and the sustainability of medical insurance funds. The experimental results show that our proposed method leads to more efficient and more accurate identifications of abnormal co-occurrence medical visit behavior, demonstrating its high efficiency and effectiveness.

13.
ArXiv ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36118666

RESUMO

The understanding of the mechanisms of SARS-CoV-2 evolution and transmission is one of the greatest challenges of our time. By integrating artificial intelligence (AI), viral genomes isolated from patients, tens of thousands of mutational data, biophysics, bioinformatics, and algebraic topology, the SARS-CoV-2 evolution was revealed to be governed by infectivity-based natural selection. Two key mutation sites, L452 and N501 on the viral spike protein receptor-binding domain (RBD), were predicted in summer 2020, long before they occur in prevailing variants Alpha, Beta, Gamma, Delta, Kappa, Theta, Lambda, Mu, and Omicron. Recent studies identified a new mechanism of natural selection: antibody resistance. AI-based forecasting of Omicron's infectivity, vaccine breakthrough, and antibody resistance was later nearly perfectly confirmed by experiments. The replacement of dominant BA.1 by BA.2 in later March was predicted in early February. On May 1, 2022, persistent Laplacian-based AI projected Omicron BA.4 and BA.5 to become the new dominating COVID-19 variants. This prediction became reality in late June. Topological AI models offer accurate prediction of mutational impacts on the efficacy of monoclonal antibodies (mAbs).

14.
BMC Cardiovasc Disord ; 22(1): 415, 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36115958

RESUMO

BACKGROUND: Abdominal aortic aneurysms have a high mortality rate. While surgery is the preferred treatment method, the biological repair of abdominal aortic aneurysms is being increasingly studied. We performed cellular and animal experiments to investigate the simultaneous function and mechanism of fibroblast growth factor 18 and integrin ß1 in the biological repair of abdominal aortic aneurysms. METHODS: Endothelial and smooth muscle cells of rat arteries were used for the cellular experiments. Intracellular integrin ß1 expression was regulated through lentiviral transfection. Interventions with fibroblast growth factor 18 were determined according to the experimental protocol. Several methods were used to detect the expression of elastic fiber component proteins, cell proliferation, and migratory activity of endothelial and smooth muscle cells after different treatments. For animal experiments, abdominal aortic aneurysms were induced in rats by wrapping the abdominal aortae in sterile cotton balls soaked with CaCl2 solution. Fibroblast growth factor 18 was administered through tail vein injections. The local expression of integrin ß1 was regulated through lentiviral injections into the adventitia of the abdominal aortic aneurysms. The abdominal aortae were harvested for pathological examinations and tensile mechanical tests. RESULTS: The expression of integrin ß1 in endothelial and smooth muscle cells could be regulated effectively through lentiviral transfection. Animal and cellular experiments showed that fibroblast growth factor 18 + integrin ß1 could improve the expression of elastic fiber component proteins and enhance the migratory and proliferative activities of smooth muscle and endothelial cells. Moreover, animal experiments showed that fibroblast growth factor 18 + integrin ß1 could enhance the aortic integrity to withstand stretch of aortic aneurysm tissue. CONCLUSION: Fibroblast growth factor 18 + integrin ß1 improved the biological repair of abdominal aortic aneurysms in rats by increasing the expression of elastic proteins, improving the migratory and proliferative abilities of endothelial and smooth muscle cells, and improving aortic remodeling.


Assuntos
Aneurisma da Aorta Abdominal , Animais , Aorta Abdominal/patologia , Aorta Abdominal/cirurgia , Aneurisma da Aorta Abdominal/induzido quimicamente , Aneurisma da Aorta Abdominal/genética , Aneurisma da Aorta Abdominal/cirurgia , Cloreto de Cálcio , Células Endoteliais/metabolismo , Fatores de Crescimento de Fibroblastos , Integrina beta1/genética , Ratos
15.
Chem Commun (Camb) ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36093763

RESUMO

Phenyl tellurosulfide (PhS-TePh) was used to study the redox activity of the S-Te bond in lithium batteries. PhS-TePh formed a dynamic covalent network during lithiation, which provided a balance between responsiveness and stability to facilitate ion and electron transfer, enabling Li/PhS-TePh cells to achieve stable cycling and excellent rate performance in dilute electrolyte.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36093885

RESUMO

As a key component of batteries, the electrolyte determines the ion transport and interface chemistry of the cathode and anode. In this work, we develop a dual-network structured hydrogel electrolyte composed of polyacrylamide (PAM), sodium alginate (SA) and potassium iodide (KI) for solid-state zinc-air/iodide hybrid batteries. The assembled hybrid battery shows excellent renewability and a long cycling life of 110 h with a high energy efficiency of 80%. The ion-crosslinked dual-network structure endows the material with improved mechanical strength and increased ionic conductivity. More importantly, the introduction of iodine species not only offers more favorable cathodic kinetics of iodide/iodate redox than oxygen electrocatalysis but also regulates the solvation structure of zinc ions to ensure better interface stability. This work provides significant concepts for developing novel solid-state electrolytes to realize high-performance energy devices and technologies.

17.
Bioinformatics ; 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36130063

RESUMO

MOTIVATION: Cancer subtype diagnosis is crucial for its precise treatment and different subtypes need different therapies. Although the diagnosis can be greatly improved by fusing multi-omics data, most fusion solutions depend on paired omics data, which are actually weakly-paired, with different omics views missing for different samples. Incomplete multi-view learning based solutions can alleviate this issue but are still far from satisfactory because they: (i) mainly focus on shared information while ignore the important individuality of multi-omics data; (ii) cannot pick out interpretable features for precise diagnosis. RESULTS: We introduce an interpretable and flexible solution (LungDWM) for Lung cancer subtype Diagnosis using Weakly-paired Multi-omics data. LungDWM first builds an attention-based encoder for each omics to pick out important diagnostic features and extract shared and complementary information across omics. Next, it proposes an individual loss to jointly extract the specific information of each omics, and performs generative adversarial learning to impute missing omics of samples using extracted features. After that, it fuses the extracted and imputed features to diagnose cancer subtypes. Experiments on benchmark datasets show that LungDWM achieves a better performance than recent competitive methods, and has a high authenticity and good interpretability. AVAILABILITY: The code is available at http://www.sdu-idea.cn/codes.php?name=LungDWM.

18.
J Cancer ; 13(11): 3251-3257, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118520

RESUMO

Hepatocellular carcinoma (HCC) is the leading malignancy associated with cancer-related deaths worldwide. Many studies have indicated that mucin (MUC) expression plays an important role in cancer metastasis and recurrence. MUC6 expression is observed in gastric and oncocytic phenotypes and may play an important role during cancer progression. We found the level of MUC6 is lower in HCC patients but did not affect the survival of HCC patients. Therefore, in this study, we investigated the combined effect of MUC6 polymorphisms and exposure to environmental carcinogens on the susceptibility to and clinicopathological characteristics of HCC. Three single-nucleotide polymorphisms (SNPs) of MUC6 (rs61869016, rs6597947, and rs7481521) from 1197 healthy controls and 423 HCC patients were analyzed using real-time PCR. After adjusting for other co-variants, we found that carrying a CC genotype at MUC6 rs61869016 had a lower risk of developing HCC than wildtype carriers. Moreover, patients with a smoking habit who carried the C allele of rs61869016 and T allele of rs7481521 had a higher (B or C) Child-Pugh score than other genotypes, suggesting significant functional compromise and decompensated disease. Therefore, our findings suggest that genetic variations in MUC6 may corelate to HCC and indicate progression in HCC patients.

19.
World J Clin Cases ; 10(20): 6981-6990, 2022 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-36051151

RESUMO

BACKGROUND: Schwannomatosis is a rare disease characterized by multiple schwannomas of the whole body. Although benign, schwannomatosis that occurs in important areas of the body, such as the brain and spinal canal, can cause considerable disability and mortality. The disease is rare, frequent and relapsing, and this poses a diagnostic and therapeutic challenge. CASE SUMMARY: A 40-year-old male had multiple masses all over his body, starting at the age of 19. Four years prior, he started to experience a progressive decrease in muscle strength in both lower limbs and developed urinary and defecation dysfunctions, and gradual paralysis. One month prior, the patient developed pain and numbness in his left forearm. The patient had undergone five surgical procedures for this disease in our department. Based on the family history, imaging examinations, pathological biopsy and molecular biological examinations, the diagnosis of schwannomatosis was confirmed. This time, the patient was admitted to our hospital again for a 6th operation because of the pain and numbness in his left forearm. After the operation, the patient's symptoms improved significantly; the patient recovered and was discharged from the hospital. At the last telephone follow-up, the patient reported a poor general condition but was alive. CONCLUSION: Here, we report a rare case of schwannomatosis. We conducted 15 years of patient follow-up and treatment, and analyzed the timing of surgery and patient psychology. This case will further extend our overall understanding of the diagnosis and treatment of this rare tumor.

20.
Front Pediatr ; 10: 959419, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36090578

RESUMO

With the rapid increase in the number of infections, children with Staphylococcus aureus (S. aureus) infection secondary to Influenza A virus (IAV), appear to have a great possibility of causing severe complications and illness. Despite some cases and research findings regarding the death of children with IAV and S. aureus, coinfection included, there were few details about successful treatment of pleural empyema and necrotizing pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA) infection following IAV. In this case report, we describe the clinical symptoms and treatment of a teenager with pleural empyema and necrotizing pneumonia related to S. aureus secondary infection who was initially infected by IAV. This case highlights the importance of early recognition and application of thoracoscopy for this potentially fatal pleural empyema caused by MRSA and IAV coinfection. We conclude that this is a significant case that contributes to raising awareness regarding rarely occurring severe respiratory infections by MRSA in a child with normal immune function after IAV. In addition, further studies are needed to explore risk factors for IAV coinfection with S. aureus.

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