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1.
Cancer Commun (Lond) ; 40(1): 3-15, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32125093

RESUMO

BACKGROUND: Indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan (Trp) catabolism have been demonstrated to play an important role in tumor immunosuppression. This study examined the expression and catalytic activity of IDO1 in penile squamous cell carcinoma (PSCC) and explored their clinical significance. METHODS: IDO1 expression level, serum concentrations of Trp and kynurenine (Kyn) were examined in 114 PSCC patients by immunohistonchemistry and solid-phase extraction-liquid chromatography-tandem mass spectrometry. The survival was analyzed using Kaplan-Meier method and the log-rank test. Hazard ratio of death was analyzed via univariate and multivariate Cox regression. Immune cell types were defined by principal component analysis. The correlativity was assessed by Pearson's correlation analysis. RESULTS: The expression level of IDO1 in PSCC cells was positively correlated with serum Kyn concentration and Kyn/Trp radio (KTR; both P < 0.001) but negatively correlated with serum Trp concentration (P = 0.001). Additionally, IDO1 up-regulation in cancer cells and the increase of serum KTR were significantly associated with advanced N stage (both P < 0.001) and high pathologic grade (P = 0.008 and 0.032, respectively). High expression level of IDO1 in cancer cells and serum KTR were associated with short disease-specific survival (both P < 0.001). However, besides N stage (hazard radio [HR], 6.926; 95% confidence interval [CI], 2.458-19.068; P < 0.001) and pathologic grade (HR, 2.194; 95% CI, 1.021-4.529; P = 0.038), only serum KTR (HR, 2.780; 95% CI, 1.066-7.215; P = 0.036) was an independent predictor for PSCC prognosis. IDO1 expression was positively correlated with the expression of interferon-γ (IFNγ, P < 0.001) and immunosuppressive markers (programmed cell death protein 1, cytotoxic T-lymphocyte-associated protein 4 and programmed death-ligand 1 and 2; all P < 0.05), and the infiltration of immune cells (including cytotoxic T lymphocytes, regulatory T lymphocytes, tumor-associated macrophages, and myeloid-derived suppressor cells; all P < 0.001) in PSCC tissues. Furthermore, the expression of IDO1 was induced by IFNγ in a dose-dependent manner in PSCC cells. CONCLUSIONS: IFNγ-induced IDO1 plays a crucial role in immunoediting and immunosuppression in PSCC. Additionally, serum KTR, an indicator of IDO1 catabolic activity, can be utilized as an independent prognostic factor for PSCC.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32155388

RESUMO

Background: Leptospira is the causative agent of leptospirosis, a zoonotic disease of global importance. To have a better understanding on the host species of Leptospira, we investigated the prevalence of Leptospira species in hedgehogs in Central China. Materials and Methods: Hedgehogs were captured in Hubei Province, China in May and October, 2018. Total DNA was extracted from the kidney tissues of hedgehogs for determining the Leptospira species by PCR amplification of the rrs2, secY, and flaB genes with genus-specific primers. Results: PCR amplification indicated that the positive rate of hedgehogs to the rrs2, secY, and flaB genes were 19.5% (8/41), 12.2% (5/41), and 9.8% (4/41), respectively. The homology of the partial sequence of rrs2, secY, and flaB genes were 99.0-100% among the Leptospira strains from hedgehogs. Phylogenetic analysis revealed that Leptospira species detected in this study clustered together with Leptospira interrogans. Conclusions: We detected L. interrogans from hedgehogs in Central China, suggesting hedgehogs are the hosts of L. interrogans.

3.
Ecotoxicol Environ Saf ; 195: 110375, 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32200142

RESUMO

Microbial immobilization is a novel and environmentally friendly technology that uses microbes to reduce metal availability in soil and accumulation of heavy metals in plants. We used urea agar plates to isolate urease-producing bacteria from the rhizosphere soil of pakchoi in Cd- and Pb-contaminated farmland and investigated their effects on Cd and Pb accumulation in pakchoi and the underlying mechanisms. The results showed that two urease-producing bacteria, Bacillus megaterium N3 and Serratia liquefaciens H12, were identified by screening. They had higher ability to produce urease (57.5 ms cm-1 min-1 OD600-1 and 76.4 ms cm-1 min-1 OD600-1, respectively). The two strains allowed for the immobilization of Cd and Pb by extracellular adsorption, bioprecipitation, and increasing the pH (from 6.94 to 7.05-7.09), NH4+ content (69.1%-127%), and NH4+/NO3- ratio (from 1.37 to 1.67-2.11), thereby reducing the DTPA-extractable Cd (35.3%-58.8%) and Pb (37.8%-62.2%) contents in the pakchoi rhizosphere soils and the Cd (76.5%-79.7%) and Pb (76.3%-83.5%) contents in the leaves (edible tissue) of pakchoi. The strains were highly resistant to heavy metal toxicity; produced IAA, siderophores and abscisic acid; and increased the NH4+/NO3- ratio, which might be related to the two strains protectiing pakchoi against the toxic effect of Cd and Pb and increasing pakchoi biomass. Thus, the results were supposed to strain resources and a theoretical basis for the remediation of Cd- and Pb-contaminated farmlands for the safe production of vegetables.

4.
Anal Chim Acta ; 1106: 96-102, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32145860

RESUMO

Peroxynitrite (ONOO-) plays a crucial role in the regulation of diverse pathophysiological processes, and high level of ONOO- is profound association with numerous diseases. Herein, we developed an anthraquinone-based fluorescent probe L for ONOO- determination by a new recognition mechanism: amido oxidized nitroso-group by ONOO-. Probe L with amine-based recognition receptor is more selective to ONOO- than other reactive oxygen species, including H2O2 and ClO-. Furthermore, ONOO- could be rapidly detected by probe L with a Limit of Detection of 13 nM. More importantly, L could be used to monitor intracellular ONOO- in SMMC-7721 cells.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32144700

RESUMO

Numerical simulations were conducted to study the effects of the pulverized-coal bias distribution in the primary air on the coal combustion and NOx generation characteristics of a 600-MW down-fired boiler with multiple-injection and multiple-staging combustion technology. The total pulverized-coal in the primary air was kept constant, and the ratio of the pulverized-coal mass flux in the fuel-rich coal/air flow to the total pulverized-coal mass flux (RPR) was set as 60%, 70%, 80%, and 90%. By changing the RPR, the excess air coefficient of the fuel-rich flow was adjusted from 0.700 to 0.467. It was found that numerical simulation results were almost in agreement with cold modeling and in situ experimental results respectively, including the flow fields in the lower furnace at the RPR of 80% and the heating processes for the fuel-rich coal/air flow at the RPR of 90%, which verified the rationality of the numerical model and the grid. The simulation results indicated that the change of RPR has little effect on the symmetry of the flow field in the furnace. With the increase of the RPR from 60 to 90%: (1) the maximum airflow declination angle near the tertiary air slot decreased from 71 to 66°, which indicates that the downward airflow penetration depth gradually decreased; (2) the ignition distance of the fuel-rich coal/air flow decreased from 1.2 to 0.9 m, and the high-temperature area in the furnace hopper decreased and the position gradually moved away from the hopper water walls; (3) the oxygen consumption rate at the initial combustion stage constantly accelerated, and the fuel NOx generation rate under the fuel-rich flow nozzle increased first and then decreased; (4) the NOx emissions at the furnace exit dropped from 778 to 662 mg/m3 at 6% O2, and the carbon in the fly ash decreased from 5.87 to 5.52%. Increasing the RPR reasonably controlled the excess air coefficient of the fuel-rich flow, and realized the high-efficiency combustion in the furnace and the reduction of NOx emissions simultaneously.

6.
Mar Drugs ; 18(2)2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32050604

RESUMO

Floridoside is a low-molecular-weight organic compound, which can be accumulated by red algae under stressful conditions to protect cells via its excellent antioxidant properties. In the present study, we investigated the antioxidant mechanism of floridoside toward human hepatocyte L-02 cells. We found that floridoside had no toxicity to L-02 cells, and no reactive oxidative species were induced by it either. However, the expression of hemoxygenase-1 (HO-1) protein was up-regulated upon exposure to floridoside, and two antioxidant enzymes, superoxide dismutase (SOD) and GSH-Px, were activated by floridoside. Moreover, we investigated the pathway involved in the production of these antioxidants, p38/extracellular signal-regulated kinase (ERK) MAPK-nuclear factor-erythroid-2-related factor 2 (Nrf2) pathway. ERK1/2 and p38 phosphorylation, nuclear translocation of Nrf2, and activation of ARE luciferase activity were observed upon exposure to floridoside. siRNA interference and inhibitor treatment suppressed the HO-1 expression and the phosphorylation of ERK1/2 and p38, respectively. These results indicated that floridoside exerted its antioxidant activity by activating HO-1 expression via p38/ERK MAPK-Nrf2 pathway in human hepatocyte L-02 cells.

7.
J Mater Chem B ; 8(10): 2123-2127, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32073103

RESUMO

Novel orange-emitting N-doped carbon dots (N-CDs) were prepared as fluorescent and colorimetric dual-mode probes for sensing nitrite (NO2-). The obtained N-CDs possessed fantastic optical properties and specific responses to NO2-. NO2- could induce the fluorescence static quenching of N-CDs, and a linear relationship was obtained in the range from 2 to 60 µM with the detection limit of 0.35 µM. More interestingly, the N-CDs exhibited ratiometric absorption signals with visible color conversion (red-purple), which allowed the development of a simple colorimetric method for NO2- monitoring in the linear range of 2-80 µM with a detection limit down to 0.13 µM. Additionally, the N-CDs were capable of monitoring NO2- in the A549 cells, which indicated their great potential in biosensing.

8.
Arch Microbiol ; 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32067064

RESUMO

Soil microbes play important roles in plant growth and in the biogeochemical cycling of earth's elements. However, the structure and functions of the microbial community associated with the growth of second-generation energy crops, such as Miscanthus, remain unclear. Thus, in this study, the composition and function of the bacterial and fungal communities associated with Miscanthus cultivation were analyzed by MiSeq sequencing combined with PICRUSt and FUNGUIld analyses. The results of community composition and diversity index analyses showed that Miscanthus cultivation significantly altered the bacterial and fungal community composition and reduced bacterial and fungal diversity. In addition, Miscanthus cultivation increased the soil organic matter (SOM) and total nitrogen (TN) contents. The correlation analysis between microbial community composition and environmental factors indicated that SOM and TN were the most important factors affecting bacterial and fungal communities. Miscanthus cultivation could enrich the abundances of Pseudomonas, Rhizobium, Luteibacter, Bradyrhizobium, Phenylobacterium and other common plant-promoting bacteria, while also increasing Cladophialophora, Hymenula, Magnaporthe, Mariannaea, etc., which predicted corresponded to the saprotrophic, plant pathogenic, and pathotrophic trophic modes. The PICRUSt predictive analysis indicated that Miscanthus cultivation altered the metabolic capabilities of bacterial communities, including the metabolism of carbon, nitrogen, and phosphorus cycle. In addition, FUNGUIld analysis indicated that Miscanthus cultivation altered the fungal trophic mode. The effects of Miscanthus on the communities and function of bacteria and fungi varied among Miscanthus species. Miscanthus specie Xiangdi NO 1 had the greatest impact on soil bacterial and fungal communities, whereas Miscanthus specie Wujiemang NO 1 had the greatest impact on soil bacteria and fungi functions. The results of this study provide a reference for the composition and function of microbial communities during the growth of Miscanthus.

9.
Artigo em Inglês | MEDLINE | ID: mdl-32011663

RESUMO

AIMS: There has been significant evolution in operative and post-transplant therapies following liver transplantation (LT). We sought to study their impact on cardiovascular mortality, particularly in the longer-term. METHODS AND RESULTS: A retrospective cohort study was conducted of all adult LTs in Australia and New Zealand across three 11-year eras from 1985 to assess prevalence, modes and predictors of early (≤30-day) and late (>30-day) cardiovascular mortality. A total of 4,265 patients were followed-up for 37,409 person-years. Overall, 1,328 patients died, and cardiovascular mortality accounted for 228 (17.2%) deaths. Both early and late cardiovascular mortality fell significantly across the eras (p < 0.001). However, cardiovascular aetiologies were consistently the leading cause of early mortality and accounted for ∼40% of early deaths in the contemporary era. Cardiovascular deaths occurred significantly later than non-cardiac aetiologies (8.8 vs 5.2 years, p < 0.001). On multivariable Cox-regression, coronary artery disease (HR 4.6,95%CI 1.2-21.6, p = 0.04) and era of transplantation (HR 0.44; 95%CI 0.28-0.70, p = 0.01) were predictors of early cardiovascular mortality while advancing age (HR 1.05, 95%CI 1.02-1.10, p = 0.005) was an independent predictors of late cardiovascular mortality. Most common modes of cardiovascular death were cardiac arrest, cerebrovascular events and myocardial infarction. CONCLUSION: Despite reductions in cardiovascular mortality post-LT over 30-years, they still account for a substantial proportion of early and late deaths. The late occurrence of cardiovascular deaths highlights the importance of longitudinal follow-up to study the efficacy of targeted risk-reduction strategies in this unique patient population.

10.
Int Immunopharmacol ; 81: 106258, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32044660

RESUMO

While imiquimod (IMQ) has been widely used in dermatology, its side effect manifested as dermatitis couldn't be ignored. However, the underlying mechanism has not been fully understood. Considering the clinical features of IMQ-related dermatitis similar to pseudo-allergic reaction and the presence of large numbers of mast cell in tissues treated with IMQ, the possibility that IMQ-related dermatitis mediated by mast cell-specific Mas-related G protein-coupled receptor X2 (MRGPRX2) should be addressed. To investigate the role of MRGPRX2 in vivo, MrgprB2, the mice homology of human MRGPRX2, was detected in IMQ-induced dermatitis mouse model. Histopathological changes including mast cell degranulation and footpad swelling were assayed in wild-type and MrgprB2-/- mice. The results showed that IMQ application induced dermatitis and footpad swelling with inflammatory cells infiltration plus mast cell activation in the skin of wild-type mice but reduced significantly in MrgprB2-/- mice. Further, compared to wild-type mice, serum histamine and inflammatory cytokine levels were compromised in MrgprB2-/- mice treated with IMQ, while the serum IgE level didn't change significantly. In vitro studies, levels of mediators released from murine peritoneal mast cells (MPMCs) after IMQ treatment were increased in a dose-dependent manner, which were much mild in MPMCs from MrgprB2-/- mice. Intracellular Ca2+ concentration was increased in a dose dependent manner after IMQ treatment both in MrgprB2-HEK293 and MRGPRX2-HEK293 cells. Moreover, ß-hexosaminidase released after IMQ treatment was blocked by siRNA directed at the MRGPRX2 receptor in LAD2 cells. In summary, MrgprB2 /MRGPRX2 mediate mast cell activation and participate in IMQ-related dermatitis.

11.
Curr Pharm Des ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32066357

RESUMO

In animals, the gastrointestinal microbiota is reported to play a major role in digestion, nutrient absorption and the release of energy through metabolism of food. Therefore, the microbiota may be a factor in the links between diet and enteric diseases and oxidative stress. The gut microbial composition and concentration are affected by diet throughout the lifetime of an animal, and respond rapidly and efficiently to dietary alterations, in particular to the use of prebiotics. Prebiotics, which play an important role in mammalian nutrition, are defined as dietary ingredients that lead to specific changes in both the composition and activity of the gastrointestinal microbiota through suppressing the proliferation of pathogens and in modifying the growth of beneficial microorganisms in the host intestine. A review of the evidence suggests possible beneficial effects of prebiotics on host intestinal health, including immune stimulation, gut barrier enhancement and the alteration of the gastrointestinal microbiota, and these effects appear to be dependent on alteration of the bacterial composition and short-chain fatty acid (SCFA) production. The production of SCFAs depends on the microbes available in the gut and the type of prebiotics available. The SCFAs most abundantly generated by gastrointestinal microbiota are acetate, butyrate and propionate, which are reported to have physiological effects on the health of the host. Nowadays, prebiotics are widely used in a range of food products to improve the intestinal microbiome and stimulate significant changes to the immune system. Thus, a diet with prebiotic supplements may help prevent enteric disease and oxidative stress by promoting a microbiome associated with better growth performance. This paper provides an overview of the hypothesis that a combination of ingestible prebiotics, chitosan, fructooligosaccharides and inulin will help relieve the dysbiosis of the gut and the oxidative stress of the host.

12.
Cell Commun Signal ; 18(1): 32, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32102665

RESUMO

BACKGROUND: Mimicking ischemia-reperfusion injury, oxygen and glucose deprivation (OGD)-re-oxygenation (OGDR) applied to endometrial cells produces significant oxidative stress and programmed necrosis, which can be inhibited by nuclear-factor-E2-related factor 2 (Nrf2) signaling. MicroRNA (miRNA)-induced repression of Keap1, a Nrf2 suppressor protein that facilitates Nrf2 degradation, is novel strategy to activate Nrf2 cascade. METHODS: MicroRNA-941 (miR-941) was exogenously expressed in HESC and primary human endometrial cells, and the Nrf2 pathway examined by Western blotting and real-time quantitative PCR analysis. The endometrial cells were treated with OGDR, cell programmed necrosis and apoptosis were tested. RESULTS: MiR-941 is a novel Keap1-targeting miRNA that regulates Nrf2 activity. In T-HESC cells and primary human endometrial cells, ectopic overexpression of miR-941 suppressed Keap1 3'-UTR (untranslated region) expression and downregulated its mRNA/protein expression, leading to activation of the Nrf2 cascade. Conversely, inhibition of miR-941 elevated Keap1 expression and activity in endometrial cells, resulting in suppression of Nrf2 activation. MiR-941 overexpression in endometrial cells attenuated OGDR-induced oxidative stress and programmed necrosis, whereas miR-941 inhibition enhanced oxidative stress and programmed necrosis. MiR-941 overexpression and inhibition were completely ineffective in Keap1-/Nrf2-KO T-HESC cells (using CRISPR/Cas9 strategy). Restoring Keap1 expression, using an UTR-depleted Keap1 construct, abolished miR-941-induced anti-OGDR activity in T-HESC cells. Thus Keap1-Nrf2 cascade activation is required for miR-941-induced endometrial cell protection. CONCLUSIONS: Targeting Keap1 by miR-941 activates Nrf2 cascade to protect human endometrial cells from OGDR-induced oxidative stress and programmed necrosis. Video Abstract.

13.
Environ Sci Pollut Res Int ; 27(8): 8707-8718, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31912394

RESUMO

Excess Cd and Pb in agricultural soils enter the food chain and adversely affect all organisms. Therefore, it is important to find an eco-friendly way to reduce heavy metal accumulation in vegetables. We used urea agar plates to isolate urease-producing bacteria from the rhizosphere soil of lettuce in Cd- and Pb-contaminated farmland and investigated their ability to produce urease and immobilize heavy metals. The effects of these strains on the biomass, quality, and Cd and Pb accumulation of lettuce were also studied. The results showed that two urease-producing bacteria, Enterobacter bugandensis TJ6 and Bacillus megaterium HD8, were screened from the rhizosphere soil of lettuce. They had a high ability to produce urease (44.5 mS cm-1 min-1 OD600-1 and 54.2 mS cm-1 min-1 OD600-1, respectively) and IAA (303 mg L-1 and 387 mg L-1, respectively). Compared with the control, inoculation with strains TJ6 and HD8 reduced the Cd (75.3-85.8%) and Pb (74.8-87.2%) concentrations and increased the pH (from 6.92 to 8.13-8.53) in solution. A hydroponic experiment showed that the two strains increased the biomass (31.3-55.2%), improved the quality (28.6-52.6% for the soluble protein content and 34.8-88.4% for the vitamin C (Vc) content), and reduced the Cd (25.6-68.9%) and Pb (48.7-78.8%) contents of lettuce shoots (edible tissue). In addition, strain HD8 had a greater ability than strain TJ6 to reduce lettuce Cd and Pb uptake and water-soluble Cd and Pb levels in solution. These data show that the urease-producing bacteria protect lettuce against Cd and Pb toxicity by extracellular adsorption, Cd and Pb immobilization, and increased pH. The effects of heavy metal immobilization by the two strains can guarantee vegetable safety in situ for the bioremediation of heavy metal-polluted farmland.

14.
Arch Microbiol ; 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31894394

RESUMO

Network analysis has contributed to studies of the interactions of microorganisms and the identification of key populations. However, such analysis has rarely been conducted in the study of reservoir bacterioplankton communities. This study investigated the bacterioplankton community composition in the surface water of the Danjiangkou Reservoir using the Illumina MiSeq sequencing platform. We observed that the bacterioplankton community primarily consisted of 27 phyla and 336 genera, including Actinobacteria, Proteobacteria, and Bacteroidetes, demonstrating the richness of the community composition. Redundancy analysis of the bacterioplankton communities and environmental variables showed that the total nitrogen (TN), pH, chemical oxygen demand (COD), and permanganate index (CODMn) were important factors affecting the bacterioplankton distribution. Network analysis was performed using the relative abundances of bacterioplankton based on the phylogenetic molecular ecological network (pMEN) method. The connectivity of node i within modules (Zi), the connectivity of node i among modules (Pi), and the number of key bacteria were high at the Taizishan and Heijizui sites, which were associated with higher TN contents than at the other sites. Among the physicochemical properties of water, TN, ammonia nitrogen (NH4-N), pH, COD, and dissolved oxygen (DO) might have great influences on the functional units of the bacterial communities in bacterioplankton molecular networks. This study improves the understanding of the structure and function of bacterioplankton communities in the Danjiangkou Reservoir.

15.
J Mol Model ; 26(2): 34, 2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31982972

RESUMO

A series of dyes are designed by adding the different electron-donating (-CH3, -NH2, -OH) and electron-withdrawing groups (-Br, -Cl, -NO2) to the different ancillary ligands in the alkynylrhenium(I) tricarbonyl diimine complexes [Re(CO)3(N^N){C≡C-C6H4-CH=C(CN)(COOH)}], where N^N = 1,10-phenanthroline (phen)(1) and then investigated the sensitization properties of dyes linked to the TiO2(101) surface. Density functional theory (DFT) and time-dependent density functional theory (TD-DFT) were used to study the electronic structure, frontier molecular orbitals, and absorption spectral properties. The effect of group-substitution on sensitization properties is obvious. When the dye molecules are combined with TiO2(101) surface, not only the absorptions of some sensitizers containing -CH3 or -OH groups have red shift but also the electrons can be directly injected into the TiO2 conduction band from the dye molecules compared with the parent molecular 1. The results indicate that the designed dyes containing electron-donating groups have smaller energy gaps, better light-harvesting efficiency, sufficient driving force, and higher charge transfer efficiency as appropriate dye sensitizers. We hope it can provide valuable hints so that we can design more efficient dye sensitizers in DSSCs.

16.
Sensors (Basel) ; 20(2)2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31936791

RESUMO

High spatial resolution remote sensing image (HSRRSI) data provide rich texture, geometric structure, and spatial distribution information for surface water bodies. The rich detail information provides better representation of the internal components of each object category and better reflects the relationships between adjacent objects. In this context, recognition methods such as geographic object-based image analysis (GEOBIA) have improved significantly. However, these methods focus mainly on bottom-up classifications from visual features to semantic categories, but ignore top-down feedback which can optimize recognition results. In recent years, deep learning has been applied in the field of remote sensing measurements because of its powerful feature extraction ability. A special convolutional neural network (CNN) based region proposal generation and object detection integrated framework has greatly improved the performance of object detection for HSRRSI, which provides a new method for water body recognition based on remote sensing data. This study uses the excellent "self-learning ability" of deep learning to construct a modified structure of the Mask R-CNN method which integrates bottom-up and top-down processes for water recognition. Compared with traditional methods, our method is completely data-driven without prior knowledge, and it can be regarded as a novel technical procedure for water body recognition in practical engineering application. Experimental results indicate that the method produces accurate recognition results for multi-source and multi-temporal water bodies, and can effectively avoid confusion with shadows and other ground features.

17.
Arch Oral Biol ; 111: 104638, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31901573

RESUMO

OBJECTIVE: The purpose of this study was to investigate the effects of Lemon essential oil (LEO) and Limonene (LIM) in the progress of early caries. DESIGN: LEO and LIM were selected as experimental medicine, while sodium fluoride (NaF) and deionized water (DW) were positive and blank controls, respectively. Bovine incisors were used to establish enamel and dentin early caries models by demineralization method in vitro. Then specimens were subjected to pH cycling. Calcium and phosphate release of demineralizing solution were measured by an automatic biochemical analyzer; Surface microhardness tester and energy dispersive X-ray spectrometer were used to detect the surface microhardness recovery and calcium- phosphate ratio on tooth surface; Degraded collagen matrix by collagenase was investigated by assaying hydroxyproline. RESULTS: Calcium release of dentin demineralizing solution of LEO group was lower than DW group's and higher than NaF group's. Both of LEO and LIM groups, the surface microhardness recovery were significantly lower than those of NaF group, which were similar to DW group. Dentin surface calcium- phosphate ratio of LEO and LIM groups were lower than those of NaF group and higher than those of DW group. Hydroxyproline concentration in the remineralizing solution of LEO and LIM groups were lower than DW groups' and higher than NaF groups'. CONCLUSIONS: LEO and LIM have influence on the progress of dentin early caries, which can stabilize its structure by inhibiting collagen degradation. Meanwhile, these medicines may provide a new drug choice for the prevention and treatment of early root caries.


Assuntos
Cariostáticos/farmacologia , Cárie Dentária , Esmalte Dentário/efeitos dos fármacos , Limoneno/farmacologia , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Animais , Bovinos , Concentração de Íons de Hidrogênio , Fluoreto de Sódio/farmacologia , Desmineralização do Dente , Remineralização Dentária
18.
Int J Urol ; 27(1): 76-82, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31663174

RESUMO

OBJECTIVE: To investigate the predictive value of common preoperative laboratory variables in patients undergoing bilateral inguinal lymph node dissection surgery for penile squamous cell carcinoma. METHODS: We retrospectively analyzed the records of 228 patients who had bilateral inguinal lymph node dissection for penile squamous cell carcinoma to assess the following clinical factors: preoperative laboratory measurements, white blood cell count, platelet count, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, serum calcium, total protein, globulin, pathological factors and survival rates after surgery. RESULTS: The percentage of positive lymph nodes was 52.6%. Univariate analysis showed that the tumor stage and grade, the presence of metastasis, white blood cell count, platelet count, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and globulin were significantly associated with the disease-specific survival (all P < 0.05). At multivariate analysis, only the neutrophil-to-lymphocyte ratio had an independent effect (hazard ratio 2.131; P = 0.035). The predictive accuracy of the neutrophil-to-lymphocyte ratio was the best among the laboratory variables. The predictive accuracy of the basic pathological factors was significantly increased by incorporating the neutrophil-to-lymphocyte ratio prognosticator. CONCLUSION: The neutrophil-to-lymphocyte ratio before inguinal lymph node dissection might be useful for predicting the prognosis of patients with penile squamous cell carcinoma.

19.
Am J Clin Oncol ; 43(1): 58-63, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31651452

RESUMO

OBJECTIVE: Long-lasting control is rarely achieved with tyrosine kinase inhibitors (TKI) alone in metastatic renal cell carcinoma (mRCC). Our study aimed to investigate the survival outcomes of adding stereotactic body radiotherapy (SBRT) to TKI in mRCC. MATERIALS AND METHODS: From September 2015 to September 2018, 56 patients treated with TKI received SBRT for 103 unresectable lesions. A total of 24 and 32 patients were irradiated before and after TKI failure, respectively. Overall survival (OS) was calculated from metastases. Progression-free survival (PFS) was calculated from SBRT. RESULTS: Overall, 10, 32, and 12 patients had International Metastatic Renal Cell Carcinoma Database Consortium favorable, intermediate, and poor risk. Median follow-up was 21.7 months (range, 5.1 to 110.6 mo). Median OS was 61.2 months. The median PFS was 11.5 months, while the 2-year LC rate was 94%. Sixteen (34%) lesions achieved complete response (CR) in patients irradiated before TKI failure, whereas only 4 (7%) lesions yielded CR in those irradiated after TKI failure (P=0.001). The median PFS in CR group was significantly longer than that of non-CR group (18.9 vs. 7.1 mo; P=0.003). The 5-year OS in CR group was 86%, compared with 48% in non-CR group (P=0.010). Four (7%) patients experienced Grade 3 toxicity. CONCLUSIONS: Adding SBRT to TKI is safe and seems to improve survival in mRCC. Patients irradiated before TKI failure have higher CR rate, and the favorable local response might turn into survival benefit.

20.
Am J Med ; 133(3): 311-322.e5, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31473150

RESUMO

BACKGROUND: New-onset postoperative atrial fibrillation is well recognized to be an adverse prognostic marker in patients undergoing noncardiac surgery. Whether postoperative atrial fibrillation confers an increased risk of stroke remains unclear. METHODS: A systematic review and meta-analysis was performed to assess the risk of stroke after postoperative atrial fibrillation in noncardiac surgery. MEDLINE, Cochrane, and EMBASE databases were searched for articles published up to May 2019 for studies of patients undergoing noncardiac surgery that reported incidence of new atrial fibrillation and stroke. Event rates from individual studies were pooled and risk ratios (RR) were pooled using a random-effects model. RESULTS: Fourteen studies of 3,536,291 patients undergoing noncardiac surgery were included in the quantitative analysis (mean follow-up 1.4 ± 1 year). New atrial fibrillation occurred in 26,046 (0.74%), patients with a higher incidence following thoracic surgery. Stroke occurred in 279 (1.5%) and 6199 (0.4%) patients with and without postoperative atrial fibrillation, respectively. On pooled analysis, postoperative atrial fibrillation was associated with a significantly increased risk of stroke (RR 2.51; 95% confidence interval, 1.76-3.59), with moderate heterogeneity. The stroke risk was significantly higher with atrial fibrillation following nonthoracic, compared with thoracic, surgery (RR 3.09 vs RR 1.95; P = .01). CONCLUSION: New postoperative atrial fibrillation following noncardiac surgery was associated with a 2.5-fold increase in the risk of stroke. This risk was highest among patients undergoing nonthoracic noncardiac surgery. Given the documented efficacy of newer anticoagulants, randomized controlled trials are warranted to assess whether they can reduce the risk of stroke in these patients.

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