Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 675
Filtrar
1.
Gene ; 848: 146898, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36122610

RESUMO

RNA editing is a post-transcriptional modification process, the chloroplast genes of which are involved in the process of chloroplast development in plant. However, the RNA editing sites of chloroplast genes remains unknown. In this study, we identified 39 RNA editing sites in 18 chloroplast genes from chloroplast genome of C. sinensis. Furthermore, the feature, structures and specificity of RNA editing sites were systematic analyzed. The differential editing efficiency were examined at 11 RNA editing sites among C. sinensis var. sinensis 'Huabai 1', 'Baiye 1' and 'Longjing 43'. Meanwhile, we identified 10 C. sinensis MORFs from five subgroups and performed comparative analyses of chromosome locations, duplication model and expression profiles. Expression analysis showed that the expression level of CsMORF9.2 was down-regulated significantly in 'Huabai 1' albino tea cultivar. This study provides a foundation for further reveal in the role of chloroplast RNA editing in albinism process of tea leaves.


Assuntos
Albinismo , Camellia sinensis , Camellia sinensis/genética , Camellia sinensis/metabolismo , Cloroplastos/genética , Cloroplastos/metabolismo , Regulação da Expressão Gênica de Plantas , Folhas de Planta/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Edição de RNA , RNA de Cloroplastos/metabolismo
2.
Rev. bras. med. esporte ; 28(6): 702-704, Nov.-Dec. 2022.
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376756

RESUMO

ABSTRACT Introduction Lumbar muscle strain is a chronic injury to soft tissues such as the lumbar muscles, ligaments, and fascia. Functional exercise has specific applications in treating lumbar muscle injuries caused by sports. However, analyses on the treatment results in the psoas muscle are inconclusive. Objective Analyze the clinical efficacy of functional exercise in treating psoas muscle dysfunction. Methods 10 athletes diagnosed with lumbar muscle strain received continuous training with a functional exercise protocol for two weeks, five times a week. Clinical efficacy was assessed by visual analog scale for pain score and Prokin254 for proprioception ability indices before and after treatment. The article adopts a mathematical statistics analysis method to analyze the therapeutic effect of motor function exercise with SPSS 13.0. Results Patients reported a reduction of pain in the muscles under exertion after functional exercise. The results were significantly different (P<0.05). Patients' lumbar strength was significantly improved. This index has a considerable statistical difference (P<0.05). Conclusion Functional exercise showed a positive effect on the treatment of psoas muscle injury. The research results of this article can provide an effective training protocol for the rehabilitation of people with a psoas muscle strain. Evidence Level II; Therapeutic Studies - Investigating the result.


RESUMO Introdução A tensão muscular lombar é uma lesão crônica dos tecidos moles, tais como músculos lombares, ligamentos e fáscia. O exercício funcional tem certas aplicações no tratamento de lesões musculares lombares ocasionadas pelo esporte. Porém, as análises na intensidade dos resultados do tratamento no músculo psoas são inconclusivas. Objetivo Analisar a eficácia clínica do exercício funcional no tratamento da disfunção no músculo psoas. Métodos 10 atletas com diagnóstico de estiramento muscular lombar receberam treinamento contínuo com protocolo de exercícios funcionais por 2 semanas, 5 vezes por semana. A eficácia clínica foi avaliada pela escala analógica visual de score de dor e Prokin254 para índices de capacidade de propriocepção antes e depois do tratamento. O artigo adota um método de estatística matemática para analisar o efeito terapêutico do exercício da função motora com SPSS 13.0. Resultados Pacientes relataram uma redução da dor na musculatura sob esforço após o exercício funcional. Os resultados foram significativamente diferentes (P<0,05). A força lombar dos pacientes foi significativamente aprimorada. Esse índice tem diferença estatística considerável (P<0,05). Conclusão O exercício funcional revelou um efeito positivo sobre o tratamento da lesão muscular do psoas. Os resultados da pesquisa deste artigo podem fornecer um protocolo eficaz de treinamento para a reabilitação de pessoas com tensão do músculo psoas. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.


RESUMEN Introducción La distensión muscular lumbar es una lesión crónica de los tejidos blandos como los músculos lumbares, los ligamentos y la fascia. El ejercicio funcional tiene ciertas aplicaciones en el tratamiento de las lesiones musculares lumbares causadas por el deporte. Sin embargo, los análisis sobre la intensidad de los resultados del tratamiento en el músculo psoas no son concluyentes. Objetivo Analizar la eficacia clínica del ejercicio funcional en el tratamiento de la disfunción del músculo psoas. Métodos 10 atletas con diagnóstico de distensión muscular lumbar recibieron un entrenamiento continuo con un protocolo de ejercicios funcionales durante 2 semanas, 5 veces por semana. La eficacia clínica se evaluó mediante la escala analógica visual para la puntuación del dolor y el Prokin254 para los índices de capacidad de propiocepción antes y después del tratamiento. El artículo adopta un método estadístico matemático para analizar el efecto terapéutico del ejercicio de la función motora con SPSS 13.0. Resultados Los pacientes informaron una reducción del dolor en los músculos bajo esfuerzo después del ejercicio funcional. Los resultados fueron significativamente diferentes (P<0,05). La fuerza lumbar de los pacientes mejoró significativamente. Este índice tiene una diferencia estadística considerable (P<0,05). Conclusión El ejercicio funcional reveló un efecto positivo en el tratamiento de la lesión del músculo psoas. Los resultados de la investigación de este artículo pueden proporcionar un protocolo de entrenamiento eficaz para la rehabilitación de personas con distensión del músculo psoas. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

3.
Brain Sci ; 12(11)2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36421858

RESUMO

It is commonly believed that alertness and attention decrease after sleep deprivation (SD). However, there are not enough studies on the changes in psychomotor vigilance testing (PVT) during SD and the corresponding changes in brain function and brain structure after SD. Therefore, we recruited 30 healthy adult men to perform a 36 h acute SD experiment, including the measurement of five indicators of PVT every 2 h, and analysis of cerebral blood flow (CBF) and grey matter volume (GMV) changes, before and after SD by magnetic resonance imaging (MRI). The PVT measurement found that the mean reaction time (RT), fastest 10% RT, minor lapses, and false starts all increased progressively within 20 h of SD, except for major lapses. Subsequently, all indexes showed a significant lengthening or increasing trend, and the peak value was in the range of 24 h-32 h and decreased at 36 h, in which the number of major lapses returned to normal. MRI showed that CBF decreased in the left orbital part of the superior frontal gyrus, the left of the rolandic operculum, the left triangular part, and the right opercular part of the inferior frontal gyrus, and CBF increased in the left lingual gyrus and the right superior gyrus after 36 h SD. The left lingual gyrus was negatively correlated with the major lapses, and both the inferior frontal gyrus and the superior frontal gyrus were positively correlated with the false starts. Still, there was no significant change in GMV. Therefore, we believe that 36 h of acute SD causes alterations in brain function and reduces alert attention, whereas short-term acute SD does not cause changes in brain structure.

4.
Front Physiol ; 13: 1054773, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388120

RESUMO

To clarify the hormone control on sex determination and differentiation, we studied the Chinese mitten crab, Eriocheir sinensis (Henri Milne Edwards, 1854), a species with importantly economic and ecological significance. The crustacean female sex hormone (CFSH) and the insulin-like androgenic gland hormone (IAG) have been found to be related to the sex determination and/or differentiation. CFSH-1 of E. sinensis (EsCFSH-1) encoded a 227 amino-acid protein including a signal peptide, a CFSH-precursor-related peptide, and a mature CFSH peptide. Normally, EsCFSH-1 was highly expressed in the eyestalk ganglion of adult female crabs, while the expression was declined in the intersex crabs (genetic females). The intersex crabs had the androgenic glands, and the expression level of EsIAG was close to that of male crabs. During the embryogenesis and larval development, the changes of EsCFSH-1 and EsIAG genes expression in male and female individuals were shown after the zoea IV stage. Next, we confirmed the existence of the regulatory feedback loop between EsCFSH-1 and EsIAG by RNA interference experiment. The feminization function of EsCFSH-1 was further verified by examining the morphological change of external reproductive organs after EsCFSH-1 knockdown. The findings of this study reveal that the regulatory interplay between CFSH and IAG might play a pivotal role in the process of sex determination and/or differentiation in decapod crustaceans.

5.
Front Plant Sci ; 13: 1020706, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388573

RESUMO

The MADS-box gene family plays a crucial role in multiple developmental processes of plants, especially in floral organ specification and the regulation of fruit development and ripening. Juglans mandshurica is a precious fruit material whose quality and yield are determined by floral organ development. The molecular mechanism of J. mandshurica female and male flower development depending on MADS-box genes remains unclear. In our study, 67 JmMADS genes were identified and unevenly distributed on 15 of 16 J. mandshurica chromosomes. These genes were divided into two types [type I (Mα, Mγ, Mδ) and type II (MIKC)]. The gene structure and motif analyses showed that most genes belonging to the same type had similar gene structures and conserved motifs. The analysis of syntenic relationships showed that MADS-box genes in J. mandshurica, J. sigillata, and J. regia exhibited the highest homology and great collinearity. Analysis of cis-acting elements showed that JmMADS gene promoter regions contained light, stress and hormone response cis-acting elements. The gene expression patterns demonstrated that 30 and 26 JmMADS genes were specifically expressed in the female and male flowers, respectively. In addition, 12 selected genes common to J. mandshurica female and male flowers were significantly upregulated at the mature stage and were used to validate the reliability of the transcriptome data using quantitative real-time PCR. This comprehensive and systematic analysis of J. mandshurica MADS-box genes lays a foundation for future studies on MADS-box gene family functions.

6.
Cancer Cell Int ; 22(1): 368, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36424626

RESUMO

Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by poor prognosis, early recurrence, and the lack of durable chemotherapy responses and specific targeted treatments. In this preclinical study, we examines Tiliroside (TS, C30H26O13), as one of the major compounds of Tribulus terrestris L. which has been used as an alternative therapy in clinic practice of breast cancer treatment, for its therapeutic use in TNBC. The association between CAXII expression level and survival probability of TNBC patients, and the difference of CAXII expression level between TNBC and normal samples were evaluated by using publicly accessible databases. To determine the anticancer efficacy of TS on TNBC cells, cell proliferation, wound healing, cell invasion, and 3D spheroid formation assays were performed and excellent anticancer activities of TS were displayed. Mouse models further demonstrated that TS significantly reduced the tumor burden and improved survival rate. The properties of TS as a novel CAXII inhibitor have also been evaluated by CAXII activity assay, pHi, pHe and lactate level assay. Further RT-PCR and Caspase-3 activity analyses also revealed the positive regulating effects of TS on E2F1,3/Caspase-3 axis in TNBC cells cultured in 2D or 3D systems. The findings indicate that TS suppresses TNBC progression as a potential novel CAXII inhibitor in preclinical experiments, which warrants further investigation on its therapeutic implications.

7.
Int J Mol Med ; 50(6)2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36367168

RESUMO

The activation of mammalian ste20­like kinase1 (Mst1) is a crucial event in cardiac disease development. The inhibition of Mst1 has been recently suggested as a potential therapeutic strategy for the treatment of diabetic cardiomyopathy. However, whether silencing Mst1 also protects against hypertensive (HP) myocardial injury, or the mechanisms through which this protection is conferred are not yet fully understood. The present study aimed to explore the role of Mst1 in HP myocardial injury using in vivo and in vitro hypertension (HP) models. Angiotensin II (Ang II) was used to establish HP mouse and cardiac microvascular endothelial cell (CMEC) models. CRISPR/adenovirus vector transfection was used to silence Mst1 in these models. Using echocardiography, hematoxylin and eosin staining, Masson's trichrome staining, the enzyme­linked immunosorbent assay detection of inflammatory factors, the enzyme immunoassay detection of oxidative stress markers, terminal deoxynucleotidyl transferase dUTP nick­end labeling staining, scanning electron microscopy, transmission electron microscopy, as well as immunofluorescence and western blot analysis of the autophagy markers, p62, microtubule­associated proteins 1A/1B light chain 3B and Beclin­1, it was found that Ang II induced HP myocardial injury with impaired cardiac function, increased the expression of inflammatory factors, and elevated oxidative stress in mice. In addition, it was found that Ang II reduced autophagy, enhanced apoptosis, and disrupted endothelial integrity and mitochondrial membrane potential in cultured CMECs. The silencing of Mst1 in both in vivo and in vitro HP models attenuated the HP myocardial injury. On the whole, these findings suggest that Mst1 is a key contributor to HP myocardial injury through the regulation of cardiomyocyte autophagy.


Assuntos
Traumatismos Cardíacos , Hipertensão , Animais , Camundongos , Angiotensina II/metabolismo , Apoptose/genética , Autofagia/genética , Células Endoteliais , Traumatismos Cardíacos/metabolismo , Hipertensão/metabolismo , Camundongos Knockout , Miócitos Cardíacos/metabolismo
8.
Clin Med Insights Oncol ; 16: 11795549221134735, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387609

RESUMO

Background: Although the clinical application of osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), has been a new step forward in the first-line treatment of non-small cell lung cancer (NSCLC), an increasing number of patients with progression on osimertinib represents a great challenge clinically. The patterns of resistance mechanisms and subsequent treatment strategies after first-line osimertinib resistance are not well established. Methods: Between January 1, 2016 and October 31, 2020, a consecutive of 56 EGFR-mutant lung cancer patients treated with osimertinib as first-line therapy at Daping Hospital (Chongqing, China) were retrospective screened. The samples of pre-osimertinib and osimertinib-resistance were all detected by next-generation sequencing (NGS) panels. Statistical analyses were carried out using SPSS 23.0 software. Survival analyses were performed using the Kaplan-Meier method and compared using a log-rank test between groups. Results: Among 47 patients with osimertinib effectiveness analysis, the median progression free survival (mPFS) was 15.4 months (95% confidence interval [CI]: 12.2-24.9 months), and median overall survival (mOS) was 35.5 months (95% CI: 23.9 months -NA). A total of 21 patients underwent repeated NGS tests upon osimertinib resistance. MET amplification was the most common resistance mechanism (6/21, 28.6%), followed by C797S mutation (5/21, 23.8%). A total of 15 patients received subsequent treatments, with mPFS of 7.3 months (95% CI 5.0 months -NA). Among them, 7 patients with EGFR C797 S or/and MET amplification received subsequent second-line targeted therapy, achieving mPFS of 7.3 months (95% CI 4.5 months -NA). Of note, 3 patients received immunotherapy as second- or third-line treatment after osimertinib resistance, achieving median clinical benefit of 37.3 months. Conclusions: MET amplification and C797S mutation are main resistance mechanisms, which could be targeted by crizotinib and gefitinib, respectively. More than 50% patients could receive subsequent anticancer targetable therapies after first-line osimertinib resistance. Immunotherapy may also be an acceptable choice after osimertinib resistance.

9.
Sensors (Basel) ; 22(22)2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36433405

RESUMO

Olfactory-induced emotion plays an important role in communication, decision-making, multimedia, and disorder treatment. Using electroencephalogram (EEG) technology, this paper focuses on (1) exploring the possibility of recognizing pleasantness induced by different concentrations of odors, (2) finding the EEG rhythm wave that is most suitable for the recognition of different odor concentrations, (3) analyzing recognition accuracies with concentration changes, and (4) selecting a suitable classifier for this classification task. To explore these issues, first, emotions induced by five different concentrations of rose or rotten odors are divided into five kinds of pleasantness by averaging subjective evaluation scores. Then, the power spectral density features of EEG signals and support vector machine (SVM) are used for classification tasks. Classification results on the EEG signals collected from 13 participants show that for pleasantness recognition induced by pleasant or disgusting odor concentrations, considerable average classification accuracies of 93.5% or 92.2% are obtained, respectively. The results indicate that (1) using EEG technology, pleasantness recognition induced by different odor concentrations is possible; (2) gamma frequency band outperformed other EEG rhythm-based frequency bands in terms of classification accuracy, and as the maximum frequency of the EEG spectrum increases, the pleasantness classification accuracy gradually increases; (3) for both rose and rotten odors, the highest concentration obtains the best classification accuracy, followed by the lowest concentration.


Assuntos
Eletroencefalografia , Odorantes , Humanos , Eletroencefalografia/métodos , Emoções , Olfato , Máquina de Vetores de Suporte
10.
Materials (Basel) ; 15(22)2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36431760

RESUMO

Composite action between the components of the concrete-filled steel tube (CFT) is complex and it is difficult to accurately obtain the experimental relationship between the steel tube and the core concrete of CFT columns. The triaxially stressed core concrete has been studied by hydrostatic test in past research, while little research has been focused on the mechanical behavior of steel tube of CFT columns. It is difficult to obtain the experimental constitutive relationship of the steel tube of CFT columns to reflect the real-time influence of biaxial stress state and local buckling of steel plate on the steel tube. To clarify the mechanical behavior of the steel tube of CFT columns, this paper proposed an elastoplastic analytical method considering biaxial stress state and local buckling of steel tube to obtain the stress-strain curve of the steel tube. This method applied the Hook's law and the plasticity theory to interpret the information conveyed by the measured vertical and hoop strain histories of the steel tube. To verify its effectiveness, 11 circular concrete-filled steel tube stub columns were fabricated and tested under axial compression. Superposition results of the axial load-strain of steel tube and core concrete were compared against the experimental curves. The widely used Sakino-Sun model of the confined concrete was adopted to calculate the axial load-strain curve of the core concrete. Satisfactory agreements between the calculated and experimental results confirmed the rationality of the proposed method in tracing the constitutive relation of the biaxially stressed steel tube even after the occurrence of the local buckling. The obtained stress-strain relationship is critical for establishment of mathematical constitutive model and finite element model of steel tube.

11.
Genes (Basel) ; 13(11)2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36421836

RESUMO

To evaluate differences among 19 different ploidy hybrid poplar clones grown in northeast China, 21 traits related to growth traits and photosynthetic characteristics were detected and analyzed. Abundant phenotypic variations exist among and within populations, and these variations are the basis of forest tree genetic improvements. In this research, variance analysis showed that the traits except the net photosynthesis rate among the different ploidies and all the other traits exhibited significant differences among the ploidies or clones (p < 0.01). Estimation of phenotypic coefficients of variation, genotypic coefficients of variation, and repeatability is important for selecting superior materials. The larger the value, the greater the potential for material selection improvement. The repeatability of the different traits ranged from 0.88 to 0.99. The phenotypic and genotypic coefficients of variation of all the investigated traits ranged from 6.88% to 57.40% and from 4.85% to 42.89%, respectively. Correlation analysis showed that there were significant positive correlations between tree height, diameter, and volume. Transpiration rate, intercellular carbon dioxide concentration, and stomatal conductance were significantly positively correlated with each other but negatively correlated with instantaneous water use efficiency. Growth traits were weakly correlated with photosynthetic indexes. The rank correlation coefficient showed that most of the growth indicators reached a significant correlation level among different years (0.40-0.98), except 1-year-old tree height with 4-year-old tree height and 1-year-old ground diameter with 3-year-old tree height, which indicated the potential possibility for early selection of elite clones. Principal analysis results showed that the contribution rate of the first principal component was 46.606%, and 2-year-old tree height, 2-year-old ground diameter, 3-year-old tree height, 3-year-old ground diameter, 3-year-old diameter at breast height, 3-year-old volume, 4-year-old tree height, 4-year-old ground diameter, 4-year-old diameter at breast height, and 4-year-old volume showed higher vector values than other traits. With the method of multiple-trait comprehensive evaluation to evaluate clones, SX3.1, SY3.1, and XY4.2 were selected as elite clones, and the genetic gains of height, basal diameter, diameter at breast height, and volume of selected clones ranged from 12.85% to 64.87% in the fourth growth year. The results showed fundamental information for selecting superior poplar clones, which might provide new materials for the regeneration and improvement of forests in Northeast China.


Assuntos
Populus , Populus/genética , Fotossíntese/genética , Árvores , Poliploidia , China , Células Clonais
12.
Pediatr Pulmonol ; 2022 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-36437396

RESUMO

The objective of this meta-analysis is to investigate the clinical efficacy of cryogenic plasma tonsillectomy for obstructive sleep apnea-hypopnea syndrome (OSAHS) in children. The literature search was conducted through China National Knowledge Infrastructure (CNKI), Wanfang Database, Embase, PubMed, and Web of Science databases. The search was from the establishment of each database to June 2022. Randomized controlled trials (RCTs) meeting the criteria for partial/total cryo-plasma tonsillectomy for treating patients with obstructive sleep apnea-hypopnea syndrome in children were included, with data extracted. The meta-analysis was performed using the Stata 16.0 and Review Manager 5.4. Seven RCTs were included in this study. The results showed that the partial/complete cryo-plasma tonsillectomy in the experimental group had a better therapeutical effective rate than the control group of patients treated with conventional surgery [Odds ratio (OR)= 2.181, 95% CI:1.306-3.645, P < 0.05]. Also, in terms of postoperative adverse reactions, the number of adverse reactions in the experimental group was significantly lower than in the control group (OR= 0.445, 95% CI:0.287-0.689, P=0.001). The analysis of surgical efficacy showed that the operative time, intraoperative blood loss, and postoperative pain score were all significantly lower than those of the control group. Furthermore, further analysis of the apnea-hypopnea index (AHI) and the lowest oxygen saturation (LSaO2) of the two groups showed that the cryo-plasma tonsillectomy treatment had higher LSaO2 levels than conventional treatment [Standardized mean difference (SMD)= 0.380, 95% CI:0.094-0.667, P=0.009].In conclusion, the application of cryo-plasma tonsillectomy can significantly improve the treatment effect of OSAHS, reducing adverse reactions. This article is protected by copyright. All rights reserved.

13.
Biomed Environ Sci ; 35(11): 1001-1011, 2022 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-36443253

RESUMO

Objective: We aimed to clarify the association between estimated pulse wave velocity (ePWV) and the changes in ePWV with all-cause mortality among middle-aged and elderly Chinese. Methods: Data were obtained from the China Health and Retirement Longitudinal Study (CHARLS) from 2011-2018. The ePWV was calculated using an equation that included age and mean blood pressure (MBP). The ΔePWV was assessed as the difference in ePWV between the first two waves. Cox proportional hazard models were used to determine the association between ePWV and ΔePWV with all-cause mortality after adjustment for potential confounders. Results: Of 13,116 participants during a median follow-up of 7.0 years, 1,356 deaths occurred. An increased ePWV was independently associated with all-cause mortality. The hazard ratio [95% confidence interval ( CI)] for participants from the 1 st-4 th quartile groups was 1.00, 1.69 (1.31-2.18), 3.09 (2.44-3.91), and 8.54 (6.78-10.75), respectively. Each standard deviation (SD) increment of ePWV increased the risk of all-cause mortality by 132%. Furthermore, the ΔePWV was significantly associated with a 1.28-fold (95% CI, 1.18-1.38) risk of all-cause mortality per SD increment. Conclusion: This cohort study provided novel evidence from a Chinese population that an increased ePWV or progression of the ePWV was independently associated with all-cause mortality, which highlighted the importance of mitigating ePWV progression in clinical practice.


Assuntos
Asiáticos , Análise de Onda de Pulso , Idoso , Pessoa de Meia-Idade , Humanos , Estudos de Coortes , Estudos Longitudinais , China/epidemiologia
14.
J Chromatogr A ; 1686: 463648, 2022 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-36410170

RESUMO

High-performance size-exclusion chromatography (HPSEC) has been developed for the rapid and quantitative analysis of inactivated foot and mouth disease virus (FMDV) and adopted by regulatory agencies and vaccine manufacturers. However, strong non-specific adsorption of type A/AKT III FMDV was found on some batches of TSK G4000 SWXL column, which significantly affected the analysis accuracy. The adsorption mechanism was studied by investigating the charge and hydrophobicity of A/AKT III FMDV and another serotype O/Mya 98, as well as several model proteins, by zeta potential and hydrophobic interaction chromatography analysis. Adsorption was related to both the FMDV strain and column lots. Some specific amino acids residues on the A/AKT III FMDV surface may strongly interact with the column if the silica-based stationary phase was not completely diol-modified. Several amino acids and chaotropic salts were screened as additives in the mobile phase to suppress the non-specific adsorption of AKT III FMDV in HPSEC analysis. Results showed that adding 0.4 M of arginine (Arg), lysine (Lys), NaClO4, or NaSCN achieved 100% FMDV recovery and normal retention time. Suppression of interaction between FMDV and the backbone of the silica matrix through competitive binding with residues of FMDV or the matrix is considered as the main mechanism by which these four additives act as suppressors. The addition of Arg, NaClO4, or NaSCN led to an apparent decrease in the thermal dissociation temperature Tm of FMDV, whereas Lys slightly increased viral stability. Finally, the mobile phase comprising 0.4 M Lys was screened as optimum that allowed accurate quantification of both two serotypes of FMDV according to method validation; particularly, a relative standard deviation (RSD) < 5% was achieved for AKT III FMDV using three different lots of columns.

15.
Food Funct ; 13(23): 12121-12134, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36321740

RESUMO

Inonotus obliquus (Fr.) Pilat is an edible mushroom which is used to produce tea and syrup due to its medicinal properties. In this study, 10 secondary metabolites (1-10), including a new lanostane triterpenoid named 2α-hydroxy-inotodiol (2α-HI, 1), were identified from the edible mushroom I. obliquus through high-resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance spectroscopy (NMR) data analysis. The neuroprotective function of all steroidal metabolites in H2O2-induced SH-SY5Y cells was investigated. The results showed that 2α-HI exhibited the most remarkable neuroprotective activity. In the meantime, 2α-HI significantly ameliorated oxidative stress damage, reactive oxygen species (ROS) accumulation and mitochondrial damage induced by H2O2 in SH-SY5Y cells. The Nrf2 siRNA and inhibitors transfected the SH-SY5Y cells, indicating the Nrf2 and BDNF/TrkB/ERK/CREB pathway mediated the neuroprotective effects of 2α-HI against the H2O2-stimulated oxidative stress and apoptosis. Moreover, the neuroprotection of 2α-HI was preliminarily verified in zebrafish. In conclusion, this research was the first to confirm that 2α-HI could effectively protect SH-SY5Y cells against H2O2-induced oxidative stress and apoptosis via the Nrf2 and BDNF/TrkB/ERK/CREB signaling pathway. Hence, this mushroom could be a potential dietary supplement to ameliorate neurodegenerative diseases.


Assuntos
Agaricales , Neuroblastoma , Fármacos Neuroprotetores , Triterpenos , Animais , Humanos , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Fármacos Neuroprotetores/farmacologia , Fator Neurotrófico Derivado do Encéfalo/genética , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Peróxido de Hidrogênio/toxicidade , Triterpenos/farmacologia , Agaricales/metabolismo , Peixe-Zebra/metabolismo , Linhagem Celular Tumoral , Estresse Oxidativo , Apoptose , Transdução de Sinais , Espécies Reativas de Oxigênio/metabolismo , Fator de Transcrição de Proteínas de Ligação GA/metabolismo
16.
Res Vet Sci ; 154: 1-7, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36375269

RESUMO

Eleutheroside E (EE) exhibits immunocompetence, antioxidant, and anti-inflammatory activity. Lipopolysaccharide (LPS) can elicit a strong immune response. In vitro experiments were used to explore whether EE protects intestinal porcine jejunum epithelial cells (IPEC-J2) barriers from LPS stress. The experiment was divided into group C (control group: complete medium), group E (group C + 0.1 mg/mL EE), group L (group C + 10 µg/mL LPS), and group EL (adding 0.1 mg/mL EE for 6 h, and then adding 10 µg/mL LPS for culture). Finally, the cell proliferation, permeability, mRNA expression of cytokines, mRNA and protein expression of tight junctions (TJs) were analyzed. The result show that, when compared to the C group, EE significantly promoted the proliferation of IPEC-J2 at 58 h and showed low permeability (P < 0.05), the anti-inflammatory cytokines IL-10 and TGF-ß mRNA expression were increased extremely significantly, the inflammatory cytokines IL-6, TNF-α, and IFN-γ mRNA expression were extremely significantly decreased (P < 0.01), the mRNA and protein expression of TJ were significantly increased in group E (P < 0.05). However, LPS showed a damaging effect. EL group compared with L group, the cell index (CI) value was higher at 58 h (P < 0.05), the permeability was significantly lower (P < 0.05), the mRNA expressions of the inflammatory cytokines were down-regulated(P < 0.01), and the TJ mRNA and protein relative expression were increased (P < 0.05). In summary, the addition of EE protects the LPS-induced increase in permeability of IPEC-J2, potentially by expressing high levels of TJ proteins and inhibiting the increase of inflammatory cytokines.

17.
Cancer Med ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36341686

RESUMO

BACKGROUND: Plasma and tissue biopsy have both used for targeting actionable driver gene mutations in lung cancer, whose concordance is imperfect. A reliable method to predict the concordance is urgently needed to ease clinical application. METHODS: A total of 1012 plasma samples, including 519 with paired-tissue biopsy samples, derived from lung adenocarcinoma patients were retrospectively enrolled. We assessed the associations of several clinicopathological characteristics and serum tumor markers with the concordance between plasma and tissue biopsies. RESULTS: When carcinoembryonic antigen (CEA) levels were higher than thresholds of 15.01 ng/ml and 51.15 ng/ml, the positive predictive value of concordance reached 90% and 95%, respectively. When CEA levels were lower than thresholds of 5.19 ng/ml and 3.26 ng/mL, the negative predictive value of concordance reached 45% and 50%. The performance of CYFRA21-1 in predicting concordance was similar but inferior to CEA (AUC: 0.727 vs. 0.741, p = 0.633). The performance of CEA combined with CYFRA21-1 in predicting the concordance was similar to that of the combination of independent factors derived from the LASSO regression model (AUC: 0.796 vs. 0.818, p = 0.067). CEA (r = 0.47, p < 0.01) and CYFRA21-1 levels (r = 0.45, p < 0.05) were significantly correlated with the maximum variant allele frequency, respectively. CONCLUSIONS: CEA combined with CYFRA21-1 could effectively predict the concordance between plasma and tissue biopsies, which could be used for evaluating the priority of plasma and tissue biopsies for gene testing to timely guide clinical applications in advanced lung adenocarcinoma patients.

18.
Front Plant Sci ; 13: 1019513, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36226295

RESUMO

Cytoplasmic male sterility (CMS) is widely used in cruciferous vegetables hybrid breeding. The C5-type CMS cabbage line exhibits stable male sterility and offers great value for cabbage breeding. However, the underlying CMS mechanism remains unclear. Here, the complete mitochondrial genome was sequenced and assembled for this line. The genome size was 221,862 bp. Mitochondrial genome comparison showed that the mitochondrial genome was likely generated by recombination with a nap-type CMS B. napus strain. Sixty-seven unknown-function open reading frames (ORFs) were identified. Seven orfs, orf114a, orf123a, orf188a, orf222a, orf261a, orf286a, and orf322a, were specifically identified in this genome. The presence of these candidate CMS genes decreased ATPase activity and ATP content by affecting the transcript levels of energy metabolism-related genes and F1F0-ATP synthase assembly. Among them, orf188a, orf222a, orf261a, orf286a, and orf322a possessed a transmembrane structure, and orf188a was cotranscribed with rps7 and trnfM. orf222a was partially homologous to atp8 and coexpressed with nad5. orf261a and orf322a were cotranscribed with cox1 and atp9, respectively. Additionally, orf114a was cotranscribed with atp8. Yeast two-hybrid assays showed that the ORF222a protein interacts with a B. oleracea ATP17 homolog (Bo7g114140) during F0-type ATP synthase assembly, reducing the quantity and activity of assembled F1F0-ATP synthase. Cytological sections showed that premature separation of the tapetum from the connective tissue and delayed tapetal programmed cell death (PCD) might be the immediate causes of CMS in C5-type CMS cabbage lines. Our results may help uncover the molecular mechanism of C5-type CMS in B. oleracea from the perspectives of the whole mitochondrial genome and cytology of anther development.

19.
Appl Opt ; 61(15): 4486-4493, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-36256288

RESUMO

Polymer stabilized cholesteric liquid crystal broadband reflective films are prepared by the thermal diffusion of a benzotriazoles organic ultraviolet (UV) absorber (UV-327) combined with photopolymerization. A gradient of UV intensity is established in the direction of film thickness, inducing the formation of a pitch gradient distribution, thus broadening the reflected bandwidth. The effects of UV-327 concentration, UV irradiation intensity, irradiation time, polymerization temperature, and C6M concentration on the reflected bandwidth of the sample are examined in detail. The results indicate that the diffusion method of the organic UV absorber enables the reflected bandwidth to be broadened under appropriate conditions.

20.
Cell Res ; 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36307579

RESUMO

Pituitary neuroendocrine tumor (PitNET) is one of the most common intracranial tumors. Due to its extensive tumor heterogeneity and the lack of high-quality tissues for biomarker discovery, the causative molecular mechanisms are far from being fully defined. Therefore, more studies are needed to improve the current clinicopathological classification system, and advanced treatment strategies such as targeted therapy and immunotherapy are yet to be explored. Here, we performed the largest integrative genomics, transcriptomics, proteomics, and phosphoproteomics analysis reported to date for a cohort of 200 PitNET patients. Genomics data indicate that GNAS copy number gain can serve as a reliable diagnostic marker for hyperproliferation of the PIT1 lineage. Proteomics-based classification of PitNETs identified 7 clusters, among which, tumors overexpressing epithelial-mesenchymal transition (EMT) markers clustered into a more invasive subgroup. Further analysis identified potential therapeutic targets, including CDK6, TWIST1, EGFR, and VEGFR2, for different clusters. Immune subtyping to explore the potential for application of immunotherapy in PitNET identified an association between alterations in the JAK1-STAT1-PDL1 axis and immune exhaustion, and between changes in the JAK3-STAT6-FOS/JUN axis and immune infiltration. These identified molecular markers and alternations in various clusters/subtypes were further confirmed in an independent cohort of 750 PitNET patients. This proteogenomic analysis across traditional histological boundaries improves our current understanding of PitNET pathophysiology and suggests novel therapeutic targets and strategies.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...