Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.450
Filtrar
1.
Sci Total Environ ; 805: 150381, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34818786

RESUMO

Suspended sediments in the estuary play an important role in regulating the erosion-accretion and shaping the geomorphological patterns. Yet, it can be strongly impacted by the human activities. Constructions of basin and estuarine engineering in the Yangtze River Estuary (YRE), including constructions of Three Gorges Dam (TGD) further upstream of the Yangtze River and Deep Waterway Project (DWP) in the estuary, have greatly modified the distribution of estuarine suspended sediment concentration (SSC). In this study, we retrieved the surface water SSC in the YRE from a total of 149 Landsat-TM/ETM+/OLI images (from 1984 to 2020) and 515 GOCI images (from 2011 to 2020) to obtain its long-term dynamics. The results indicate that the SSC estimation model performed well with a mean absolute percentage error of 12.83% and a root mean square error of 0.027 g/L. The SSC in the YRE demonstrated an overall declining pattern over the past 37 years, particularly during the flood season, which was related to the decrease in the sediment discharge of Yangtze River. The SSC in different sub-regions across the entire YRE responded differently to the reduced sediment discharge caused by the impoundment of the TGD. The SSC in the inner estuary responded promptly with a 40.3% decline noted immediately after the impoundment of the TGD, whereas hysteresis of SSC changes was found in the estuarine turbidity maximum zone (TMZ) and have begun to decline by 20.0-30.0% in the recent 5 years. Constructions of local estuarine engineering, such as the DWP also exert important controls on the estuarine SSC dynamics by blocking the lateral transport of suspended sediments between the shoal and the river channel. Our results highlight the strong influence of engineering constructions on the SSC dynamics in the world large river-influenced estuaries.


Assuntos
Estuários , Poluentes Químicos da Água , China , Monitoramento Ambiental , Sedimentos Geológicos , Humanos , Rios , Poluentes Químicos da Água/análise
2.
J Colloid Interface Sci ; 607(Pt 2): 1944-1952, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34695743

RESUMO

In order to realize outstanding electrochemical performance in oxygen evolution reactions (OER), it is important to construct 3D hierarchical nanospheres consisting of 2D bimetal metal-organic framework (MOF) nanosheets. Based on high-throughput density-functional theory (DFT) calculations, we chose Ni and V as central ions and prepared Ni-V bimetal MOFs nanospheres (NiV-MNs) assembled from ultrathin 2D MOFs nanosheets through a simple one-step solvothermal method. So far, V-based ultrathin 2D MOFs have been firstly reported. Gradient experiments demonstrated that NiV-MNs shows the best catalytic activity when the amount of Ni is equal to that of V (denoted as Ni1V1-MNs). The Ni1V1-MNs can deliver a high current density of 50 mA·cm-2 at a low over-potential of 370 mV in alkaline condition even after 10000 s continuous catalytic testing. Coincidentally, the DFT calculations further confirmed that the ΔG of Ni1V1-MNs is much lower compared with Ni1V2-MNs and Ni2V1-MNs. Based upon the experimental results and DFT calculations, we propose that the better performance of Ni1V1-MNs should be attributed to the grater intrinsic activity of Ni and the coupling effect between Ni and V, as they are crucial for tuning the electrochemical activity.

3.
Front Cell Infect Microbiol ; 11: 734750, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34858869

RESUMO

The biological functions of growth factor, such as granulins, have been explored in parasites, and we elucidated that Clonorchis sinensis granulin (CsGRN) promoted the metastasis of hepatocellular carcinoma in our previous study. However, it is still unclear for the malignant transformation role of CsGRN in normal human hepatocytes. In this study, by transfecting pEGFP-C1-CsGRN eukaryotic expression plasmid, a cell line with stable overexpression of CsGRN in normal hepatocyte (LO2-GRN cells) was constructed. The effects on cell proliferation were detected by carrying out cell counting kit-8 (CCK8) assay and colony formation assay. Additionally, we conducted flow cytometry analysis to determine whether the proliferation of CsGRN was due to cell cycle arrest. Subsequently, the migration ability and the invasion ability of LO2-GRN cells were evaluated through wound-healing assay and transwell assay. Meanwhile, the levels of the markers of RAS/MAPK/ERK and PI3K/Akt signaling pathways activation in LO2-GRN cells were assessed by quantitative RT-PCR and Western blot. Our results indicated that CsGRN promoted the proliferation of LO2 cells by regulating the expression of cell-cycle-related genes. Moreover, the overexpression of CsGRN regulates malignant metastasis of liver cells by inducing the upregulation of epithelial-mesenchymal transition (EMT) marker proteins. Furthermore, both mRNA and protein expression levels of p-EGFR, RAS, p-ERK, p-AKT, p-PI3K, and p-braf have been enhanced by CsGRN. These results showed that CsGRN promoted the malignant transformation of hepatocytes by regulating epidermal growth factor receptor (EGFR)-mediated RAS/MAPK/ERK and PI3K/Akt signaling pathways, which suggested that CsGRN could serve as a novel oncoprotein during Clonorchis sinensis-associated malignant transformation of hepatocytes.

4.
Innovation (N Y) ; 2(4): 100176, 2021 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-34806059

RESUMO

The great losses caused by financial fraud have attracted continuous attention from academia, industry, and regulatory agencies. More concerning, the ongoing coronavirus pandemic (COVID-19) unexpectedly shocks the global financial system and accelerates the use of digital financial services, which brings new challenges in effective financial fraud detection. This paper provides a comprehensive overview of intelligent financial fraud detection practices. We analyze the new features of fraud risk caused by the pandemic and review the development of data types used in fraud detection practices from quantitative tabular data to various unstructured data. The evolution of methods in financial fraud detection is summarized, and the emerging Graph Neural Network methods in the post-pandemic era are discussed in particular. Finally, some of the key challenges and potential directions are proposed to provide inspiring information on intelligent financial fraud detection in the future.

5.
Artigo em Inglês | MEDLINE | ID: mdl-34831662

RESUMO

During the COVID-19 pandemic, many preschool-aged children were forced to remain indoors due to social distancing measures and school closures. In this study, we examined how children's movement behaviors (sedentary behaviors, physical activity, and sleep) were affected by the pandemic. Children's (N = 25, age = 4.4 years, SD = 0.3) movement behaviors were measured before and after the COVID outbreak, respectively. Data collected using accelerometers were analyzed using compositional data analyses. A significant change in the overall time-use composition (F = 5.89, p = 0.002) was found. Results suggested that children spent more time sleeping (8% increase) and in moderate-to-vigorous physical activity (16% increase), with less time spent in sedentary behaviors (9% decrease). However, parent reports suggested that children were less active and had more screen time. In conclusion, the current evidence suggests that children's physical activity is not negatively impacted by the pandemic. However, the continuous surveillance of movement behaviors of young children during the pandemic is needed.

6.
Vet Sci ; 8(11)2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34822645

RESUMO

Porcine circovirus 2 (PCV2), considered one of the most globally important porcine pathogens, causes postweaning multisystemic wasting syndrome (PMWS). This virus is localized in the mitochondria in pigs with PMWS. Here, we identified, for the first time, a mitochondrial localization signal (MLS) in the PCV2 capsid protein (Cap) at the N-terminus. PK-15 cells showed colocalization of the MLS-EGFP fusion protein with mitochondria. Since the PCV2 Cap also contained a nuclear localization signal (NLS) that mediated entry into the nucleus, we inferred that the subcellular localization of the PCV2 Cap is inherently complex and dependent on the viral life cycle. Furthermore, we also determined that deletion of the MLS attenuated Cap-induced apoptosis. More importantly, the MLS was essential for PCV2 replication, as absence of the MLS resulted in failure of virus rescue from cells infected with infectious clone DNA. In conclusion, the MLS of the PCV2 Cap plays critical roles in Cap-induced apoptosis, and MLS deletion of Cap is lethal for virus rescue.

7.
J Healthc Eng ; 2021: 6024352, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34754409

RESUMO

Circle of Willis (CoW) is the most critical collateral pathway that supports the redistribution of blood supply in the brain. The variation of CoW is closely correlated with cerebral hemodynamic and cerebral vessel-related diseases. But what is responsible for CoW variation remains unclear. Moreover, the visual evaluation for CoW variation is highly time-consuming. In the present study, based on the computer tomography angiography (CTA) dataset from 255 patients, the correlation between the CoW variations with age, gender, and cerebral or cervical artery stenosis was investigated. A multitask convolutional neural network (CNN) was used to segment cerebral arteries automatically. The results showed the prevalence of variation of the anterior communicating artery (Aco) was higher in the normal senior group than in the normal young group and in females than in males. The changes in the prevalence of variations of individual segments were not demonstrated in the population with stenosis of the afferent and efferent arteries, so the critical factors for variation are related to genetic or physiological factors rather than pathological lesions. Using the multitask CNN model, complete cerebral and cervical arteries could be segmented and reconstructed in 120 seconds, and an average Dice coefficient of 78.2% was achieved. The segmentation accuracy for precommunicating part of anterior cerebral artery and posterior cerebral artery, the posterior communicating arteries, and Aco in CoW was 100%, 99.2%, 94%, and 69%, respectively. Artificial intelligence (AI) can be considered as an adjunct tool for detecting the CoW, particularly related to reducing workload and improving the accuracy of the visual evaluation. The study will serve as a basis for the following research to determine an individual's risk of stroke with the aid of AI.

8.
R Soc Open Sci ; 8(10): 210121, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34754491

RESUMO

Dilute magnetic semiconductors (DMSs), such as (In, Mn)As and (Ga, Mn)As prototypes, are limited to III-V semiconductors with Curie temperatures (T c) far from room temperature, thereby hindering their wide application. Here, one kind of DMS based on perovskite niobates is reported. BaM x Nb(1-x)O3-δ (M = Fe, Co) powders are prepared by the composite-hydroxide-mediated method. The addition of M elements endows BaM x Nb(1-x)O3-δ with local ferromagnetism. The tetragonal BaCo x Nb(1-x)O3-δ nanocrystals can be obtained by Co doping, which shows strong saturation magnetization (M sat) of 2.22 emu g-1, a remnant magnetization (M r) of 0.084 emu g-1 and a small coercive field (H c) of 167.02 Oe at room temperature. The ab initio calculations indicate that Co doping could lead to a 64% local spin polarization at the Fermi level (E F) with net spin DOS of 0.89 electrons eV-1, this result shows the possibility of maintaining strong ferromagnetism at room temperature. In addition, the trade-off effect between the defect band absorption and ferromagnetic properties of BaM x Nb(1-x)O3-δ is verified experimentally and theoretically.

9.
Chemosphere ; : 132785, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34742758

RESUMO

Hydrothermal carbonization (HTC) can improve biomass quality in both physical and chemical aspects for energy application. This study aims to investigate the characteristics and reactivities of rape straw (RS) hydrochars. Hydrochars were prepared at 160-240 °C with residence time of 15-120 min. Mass yield, energy yield, microstructure, functional group and migration of alkali and alkaline earth metals (AAEMs) were studied to evaluate the influence of different conditions on properties of hydrochar. The results showed that O/C and H/C ratio decreased, while the higher heating value (HHV) increased with increasing temperature and residence time. The effect of increasing temperature on hydrochar properties was more significant than residence time. The structure was changed, and hydrochar possessed a more stable form after the aromatization reaction. For the gasification reactivity of hydrochar, decomposition rate curves showed that the peak of pyrolysis and gasification moved to a higher temperature region with the increasing of HTC temperature because of the developed aromatic structures in hydrochar. The pyrolysis activation energy decreased from raw RS 71.68 to 41.03 kJ/mol in 240 °C, while gasification activation energy increased from 80.42 to 251.30 kJ/mol. Moreover, it was found that HTC can reduce the content of AAEMs efficiently and the best removal condition is 200 °C. Ca content dropped to a minimum value at 200 °C and then increased at higher temperature which may be caused by well-developed pore structure in hydrochars. This study provides basic data for comprehensive utilization of rape straw and migration mechanism of AAEMs in HTC process.

10.
Neural Netw ; 145: 121-127, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34735890

RESUMO

When training deep learning models, data augmentation is an important technique to improve the performance and alleviate overfitting. In natural language processing (NLP), existing augmentation methods often use fixed strategies. However, it might be preferred to use different augmentation policies in different stage of training, and different datasets may require different augmentation policies. In this paper, we take dynamic policy scheduling into consideration. We design a search space over augmentation policies by integrating several common augmentation operations. Then, we adopt a population based training method to search the best augmentation schedule. We conduct extensive experiments on five text classification and two machine translation tasks. The results show that the optimized dynamic augmentation schedules achieve significant improvements against previous methods.

11.
PeerJ ; 9: e12373, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34824909

RESUMO

The characteristics of solar radiation and the influence of sand and dust on solar radiation in the northern margin of Taklimakan Desert were analyzed using radiation observation data from 2018. The results showed that the annual total radiation, direct radiation, and scattered radiation at Xiaotang were 5,781.8, 2,337.9, and 3,323.8 MJ m-2, respectively. The maximum monthly total radiation, direct radiation, and scattered radiation were observed in July (679.8 MJ m-2), August (317.3 MJ m-2), and May (455.7 MJ m-2), respectively. The aerosol optical depth corresponded well with the scattered radiation, and the maximum value was in May. Further analysis showed a significant correlation between the total radiation and solar height angle under different weather conditions. Under the same solar height angle, total radiation was higher during clear days but lower on sandstorm days. Calculation of atmospheric transmittance showed that the average atmospheric transmittance on a clear day was 0.67; on sand-and-dust days, it was 0.46. When the atmospheric transmittance was 0.5, the increase in scattering radiation by aerosol in the air began to decrease. Probability analysis of radiation indicated the following probabilities of total radiation <500 W m-2 occurring on clear, floating-dust, blowing-sand, and sandstorm days: 67.1%, 76.3%, 76.1%, and 91.8%, respectively. Dust had the greatest influence on direct radiation; the probabilities of direct radiation <200 W m-2occurring on clear, floating-dust, blowing-sand, and sandstorm days were 44.5%, 93.5%, 91.3%, and 100%, respectively, whereas those of scattered radiation <600 W m-2were 100%, 99.1%, 98.1%, and 100%, respectively. Therefore, the presence of dust in the air will reduce scattered radiation.

12.
Clin Drug Investig ; 2021 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-34837169

RESUMO

BACKGROUND AND OBJECTIVE: Palbociclib is an oral small-molecule inhibitor of cyclin-dependent kinase 4/6 used for the treatment of advanced breast cancer. This study compared the pharmacokinetic and safety profiles between a new generic and a branded reference formulation of palbociclib capsules in healthy Chinese subjects under fasting and fed conditions and evaluated the bioequivalence of two palbociclib products to obtain sufficient evidence for the marketing approval of the new generic drug. METHODS: A randomized, open-label, two-period crossover study was conducted in healthy Chinese volunteers under both fasting and fed conditions (30 subjects/condition). Eligible healthy subjects received a single 125-mg dose of the palbociclib test or reference formulation followed by a 14-day washout period. Serial blood samples were collected at scheduled timepoints, and plasma concentrations were determined by a validated high-performance liquid chromatography-tandem mass spectrometry method. A non-compartment method was used to calculate the main pharmacokinetic parameters, including the area under the plasma concentration-time curve (AUC) from time 0 to the time of the last measurable concentration (AUC0-t), the AUC from time 0 to infinity (AUC0-∞), the maximum plasma concentration (Cmax), the time to maximum plasma concentration, and the elimination half-life. The geometric mean ratios and the corresponding 90% confidence intervals of palbociclib were acquired for the bioequivalence analysis. Safety and tolerability were assessed by monitoring adverse events, laboratory assessments, vital signs, physical examinations, and 12-lead electrocardiograms. RESULTS: Under the fasting condition, the pharmacokinetic parameter values of the test formulation were similar to those of the reference formulation. The 90% confidence intervals of geometric mean ratios of the test to reference formulations were 94.35-103.82% for Cmax, 94.79-103.26% for AUC0-t, and 94.82-103.38% for AUC0-∞, which are all within the accepted bioequivalence range of 80.00-125.00%. Meanwhile, under the fed condition, the pharmacokinetic parameter values of the test formulation were also similar to those of the reference formulation. The 90% confidence intervals of geometric mean ratios of the test to reference formulations were 96.65-103.56% for Cmax, 98.06-103.61% for AUC0-t, and 97.88-103.46% for AUC0-∞, which are all within the accepted bioequivalence range of 80.00-125.00%. The test and reference products were well tolerated, and no serious adverse events occurred during the study. CONCLUSIONS: Pharmacokinetic bioequivalence of palbociclib in healthy subjects was established between the palbociclib test formulation and the reference formulation under fasting and fed conditions according to predetermined regulatory criteria. The two formulations were safe and well tolerated.

13.
Chem Commun (Camb) ; 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34842255

RESUMO

A series of new photoresponsive macrocyclic anion receptors were synthesized via integration of an azobenzene unit and multiple anion binding sites. They exhibited highly selective binding to dianionic sulfate over other tested anions and the reversible release of sulfate could be triggered by visible light as inferred from mass spectroscopy, crystallographical analysis, NMR spectroscopy, and theoretical calculations.

14.
Front Genet ; 12: 754421, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721542

RESUMO

Meniscus plays an important role in joint homeostasis. Tear or degeneration of meniscus might facilitate the process of knee osteoarthritis (OA). Hence, to investigate the transcriptome change during meniscus degeneration, we reveal the alterations of messenger RNA (mRNA), microRNA (miRNA), long noncoding RNA (lncRNA), and circular RNA (circRNA) in meniscus during OA by whole-transcriptome sequence. A total of 375 mRNAs, 15 miRNAs, 56 lncRNAs, and 90 circRNAs were significantly altered in the degenerative meniscus treated with interleukin-1ß (IL-1ß). More importantly, highly specific co-expression RNA (ceRNA) networks regulated by lncRNA LOC107986251-miR-212-5p-SESN3 and hsa_circ_0018069-miR-147b-3p-TJP2 were screened out during IL-induced meniscus degeneration, unveiling potential therapeutic targets for meniscus degeneration during the OA process. Furthermore, lipocalin-2 (LCN2) and RAB27B were identified as potential biomarkers in meniscus degeneration by overlapping three previously constructed databases of OA menisci. LCN2 and RAB27B were both upregulated in osteoarthritic menisci and IL-1ß-treated menisci and were highly associated with the severity of OA. This could introduce potential novel molecules into the database of clinical diagnostic biomarkers and possible therapeutic targets for early-stage OA treatment.

15.
Front Microbiol ; 12: 770656, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777324

RESUMO

In the past two decades, coronavirus (CoV) has emerged frequently in the population. Three CoVs (SARS-CoV, MERS-CoV, SARS-CoV-2) have been identified as highly pathogenic human coronaviruses (HP-hCoVs). Particularly, the ongoing COVID-19 pandemic caused by SARS-CoV-2 warns that HP-hCoVs present a high risk to human health. Like other viruses, HP-hCoVs interact with their host cells in sophisticated manners for infection and pathogenesis. Here, we reviewed the current knowledge about the interference of HP-hCoVs in multiple cellular processes and their impacts on viral infection. HP-hCoVs employed various strategies to suppress and evade from immune response, including shielding viral RNA from recognition by pattern recognition receptors (PRRs), impairing IFN-I production, blocking the downstream pathways of IFN-I, and other evasion strategies. This summary provides a comprehensive view of the interplay between HP-hCoVs and the host cells, which is helpful to understand the mechanism of viral pathogenesis and develop antiviral therapies.

16.
CNS Neurosci Ther ; 27(12): 1561-1569, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34676982

RESUMO

BACKGROUND: Dual antiplatelet therapy is considered beneficial in acute ischemic stroke (AIS) patients with intracranial artery stenosis (ICAS), with more bleeding events. Ginkgolide is shown to reduce platelet activation after infarction, which might be of benefit in AIS. We aimed to explore the effect of Ginkgolide in AIS patients with ICAS. METHODS: This was a randomized, double-blinded, placebo-controlled trial conducted at 61 centers in China. Within 72 h after onset, consecutive patients diagnosed as AIS with ICAS were randomized to either Ginkgolide or placebo treatment. The primary outcome was the composite of mortality and recurrent stroke (ischemic or hemorrhagic) during first 4 weeks in an intention-to-treat analysis. Secondary functional outcome was assessed by modified Rankin Scale and improvement of stroke severity was assessed by National Institution of Health Stroke Scale at day 28. Safety outcome was measured by the rate of severe adverse event (SAE). RESULTS: There were 936 patients randomized to either Ginkgolide or placebo treatment. Their average age was 64.2 ± 10.4 years old and 36.0% of the patients were female. The composite index event occurred in six patients in placebo group, and none occurred in Ginkgolide group (risk ratio 1.01; 95% CI 1.00-1.02). There were more patients who achieved favorable outcome in Ginkgolide group, compared with that of the placebo group (OR 2.16, 95%CI 1.37-3.41). SAE occurred in five (1.1%) patients in the Ginkgolide group and three (0.6%) in the placebo group (OR0.60, 95CI% 0.14-2.53). Intracranial hemorrhage occurred in 1/473 (0.2%) in the placebo group. CONCLUSIONS: Ginkgolide, working as PAF antagonist, may reduce recurrent stroke in AIS with ICAS patients within 72 hours after onset. It might be an optional treatment in moderate-to-severe AIS patients with ICAS. (http://www.chictr.org.cn Number as ChiCTR-IPR-17012310).

17.
Dalton Trans ; 50(43): 15567-15575, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34668002

RESUMO

Efficient detection of toxic radioiodine species emitted by nuclear-related activities and accidents is crucial for the health of the human body and safety of the environment. Herein we report that a series of stable Ln-based MOFs with BTC linkers (Ln-BTCs) could provide dual fluorescence and electrochemical response of iodine vapor in the presence of humidity. Iodine molecules could be attracted by the oxygen sites of carboxylate linkers and confined in the cavities of Ln-BTCs. Due to the photoinduced electron transfer effect, the fluorescence of Ln-BTCs is quenched drastically in the presence of iodine. Meanwhile, the conductivity of Ln-BTCs could reach an increase of 107 fold in magnitude after iodine trapping. Water molecules could also be trapped in Ln-BTCs, having interacted with the frameworks via hydrogen bonds, and reduce the iodine uptake capacity, but they cannot alter the fluorescence and conductive properties of Ln-BTCs as distinctly as iodine molecules could. Besides, Raman mapping suggests a diffusion coefficient of 1.5 × 10-14 m2 s-1 for iodine transport in the porous Eu-BTC. The dual fluorescence and electrochemical signal outputs show high sensitivity and attractive ability to reduce false positives, which could be useful for potential integration for sensing radioiodine vapor.

18.
Shock ; 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34618729

RESUMO

ABSTRACT: Blocking ferroptosis reduces ischemia-reperfusion injury in some pathological contexts. However, there is no evidence that ferroptosis contributes to post-resuscitation myocardial dysfunction (PRMD). Here, we evaluated the therapeutic performance of ferroptosis inhibitors (UAMC-3203 or/and Deferoxamine) on the PRMD in a rat model of cardiac arrest and surveyed the changes of essential ferroptosis markers in the myocardium. Remarkably, all treatments reduce the severity of cardiac dysfunction and microcirculation hypoperfusion after resuscitation compared with control. Consistently, we observe that the ferroptosis marker Glutathione peroxidase 4, 4-hydroxynonenal and non-heme iron altered (1 ±â€Š0.060 vs. 0.021 ±â€Š0.016, 1 ±â€Š0.145 vs. 3.338 ±â€Š0.221, 52.010 ±â€Š3.587 ug/g vs. 70.500 ±â€Š3.158 ug/g, all P < 0.05) in the myocardium after resuscitation. These changes were significantly suppressed by UAMC-3203 [(0.187 ±â€Š0.043, 2.848 ±â€Š0.169, all P < 0.05), (72.43 ±â€Š4.920 ug/g, P  > 0.05)], or Deferoxamine (0.203 ±â€Š0.025, 2.683 ±â€Š0.273, 55.95 ±â€Š2.497 ug/g, all P < 0.05). Briefly, UAMC-3203 or/and Deferoxamine improve post-resuscitation myocardial dysfunction and provide evidence of ferroptosis involvement, suggesting that ferroptosis inhibitors could potentially provide an innovative therapeutic approach for mitigating the myocardial damage caused by cardiopulmonary resuscitation.

19.
Oral Oncol ; 122: 105576, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34689010

RESUMO

The metastatic rate of nasopharyngeal carcinoma (NPC) is the highest among head and neck tumours. Additionally, distant metastasis is the main cause of therapy failure and mortality in NPC. Thus, novel biomarkers are needed for designing new therapeutic strategies to improve the prognosis of this disease. In this study, qRT-PCR and western blotting revealed that the expression of the WD repeat domain phosphoinositide interacting 1 (WIPI-1) was markedly decreased in NPC cells and tissues. Furthermore, low WIPI-1 expression closely correlated with poor prognosis in NPC patients. In vitro functional experiments revealed that overexpression or knockdown of WIPI-1 repressed or facilitated the migration, colony formation, and proliferation of NPC cells. Consistent with the in vitro studies, WIPI-1 significantly inhibited tumour growth, invasion and metastasis in popliteal lymph node metastasis, lung metastasis, and xenograft mouse models in vivo. Mechanistically, WIPI-1 directly interacted with tripartite motif containing 21 (TRIM21) and enhanced starvation-induced autophagy by interacting with TRIM21 in NPC cells. Moreover, MYC gene expression was markedly increased in the WIPI-1 knockdown group, as demonstrated by RNA-seq analysis and qRT-PCR validation. Altogether, WIPI-1 acts as a tumour suppressor gene in NPC that inhibits tumour growth and metastasis. Targeting WIPI-1 may be a novel treatment approach for NPC.

20.
Medicine (Baltimore) ; 100(40): e27453, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34622867

RESUMO

RATIONALE: Functional pancreatic neuroendocrine tumors (pNETs) rarely produce vasopressin. Here, we reported a case of pNET producing vasopressin in a 78-year-old man with hyponatremia. PATIENT CONCERNS: The patient presented with anorexia approximately 4 years ago, and the laboratory test results indicated hyponatremia. He was hospitalized 3 times subsequently due to anorexia in the past 4 years, during which laboratory tests consistently indicated severe hyponatremia. DIAGNOSIS: Upon admission, his serum osmolarity, urine osmolarity, urine sodium level, and 24-hour urine sodium level was 277 mOsm/kg H2O, 465 mOsm/kg H2O, 82.5 mmol/L, and 140.25 mmol, respectively. Gallium-68-labeled tetraazacyclododecanetetraacetic acid-Dphel-Tyr3-octreotate positron emission tomography-computed tomography showed a high uptake lesion measuring approximately 1 cm in diameter in the pancreatic body, and the possibility of pNET was considered. Besides, laboratory tests showed that adrenocorticotropic hormone, follicle-stimulating hormone, and luteinizing hormone released by the pituitary was insufficient in the case of low levels of cortisol, estradiol, progesterone, and testosterone. Thus, the diagnosis of the syndrome of inappropriate antidiuresis (SIAD) was considered along with hypopituitarism. INTERVENTIONS: The patient underwent surgery, and pNET was confirmed by pathology examination. The immunohistochemical study showed that the tumor cells were positive for somatostatin receptors 2 and vasopressin. OUTCOMES: In the last follow-up 17 months after surgery, the patient was in good condition, taking methylprednisolone 4 mg every other day, and had been free of anorexia or hyponatremia episodes. LESSONS: This case illustrated the potential ectopic production of vasopressin resulting in SIAD in pNETs, highlighting the adoption of gallium-68-labeled tetraazacyclododecanetetraacetic acid-Dphel-Tyr3-octreotate positron emission tomography-computed tomography and vasopressin immunohistochemical staining in the evaluation of the etiology of SIAD.


Assuntos
Síndrome de Secreção Inadequada de HAD/etiologia , Tumores Neuroendócrinos/complicações , Neoplasias Pancreáticas/complicações , Vasopressinas/biossíntese , Corticosteroides/uso terapêutico , Idoso , Anorexia/etiologia , Humanos , Hiponatremia/etiologia , Síndrome de Secreção Inadequada de HAD/complicações , Síndrome de Secreção Inadequada de HAD/tratamento farmacológico , Masculino , Tumores Neuroendócrinos/cirurgia , Neoplasias Pancreáticas/cirurgia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...