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1.
J Affect Disord ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36707037

RESUMO

BACKGROUND: Health literacy is associated with mental disorders in adolescents. This study aimed to examine the associations between health literacy, depressive symptoms, and suicide-related outcomes. METHODS: In terms of participants, 937 middle school students in Shenyang City, China were enrolled between December 2018 and December 2019 to assess health literacy, depressive symptoms and suicide-related outcomes. Multiple logistic regression models were used to examine possible associations. RESULTS: Adequate health literacy was negatively associated with 12-month follow-up depressive symptoms (RR = 0.115, 95 % CI: 0.032-0.411) and suicide-related outcomes (RR = 0.230, 95 % CI: 0.085-0.618) in females. Meanwhile, in females, interpersonal relationship, stress management, and self-actualization were negatively related to depressive symptoms, while physical activities, self-actualization, and health awareness are protective factors for suicide-related outcomes (P < 0.05 for each). LIMITATIONS: Recall and reporting bias might be inevitable for self-reported data, and due to the small sample size, the predictive effect may not be very significant. CONCLUSIONS: These findings suggest that adequate health literacy may serve as a critical and independent protective factor for poor long-term mental health outcomes and that the predictive effect is more pronounced in female students. Public health efforts to enhance health literacy levels are required to maintain and improve adolescents' mental health outcomes.

2.
BMC Public Health ; 23(1): 142, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36670413

RESUMO

BACKGROUND: Problematic mobile phone use (PMPU) is becoming increasingly popular and has serious harmful effects on physical and mental health among adolescents. Inadequate health literacy (HL) is related to some risky behaviors and mental health problems in adolescents. Nevertheless, few studies have explored the relationship between HL and PMPU and the gender difference in the relationship among Chinese adolescents. The aim of this study was to examine the associations between HL and PMPU and explore gender difference in the associations. METHODS: A total of 22,628 junior and senior high school students (10,990 males and 11,638 females) in 6 regions of China participated in this study. HL and PMPU were measured by self-report validated questionnaires. Chi-square tests and logistic regression analysis were conducted in the study. RESULTS: Logistic regression analysis showed that students with inadequate HL are likely to have PMPU (OR = 2.013, 95% CI: 1.840-2.202), and different degrees of association can be seen in six dimensions. Besides, in both males and females, students with inadequate HL had a higher risk of PMPU (OR male = 1.607, 95% CI: 1.428-1.807; OR female = 2.602, 95% CI: 2.261-2.994). Regarding the gender difference, the results showed that males had more PMPU than females, and the difference was more significant for students with adequate HL than those with inadequate HL (OR inadequate = 1.085, 95% CI: 1.016-1.159; OR adequate = 1.770, 95% CI: 1.490-2.101). Similarly, there were associations in the six dimensions. CONCLUSIONS: HL decreases PMPU, and males have a higher risk of PMPU than females. These findings suggest a reasonable strategy to reduce PMPU by improving the HL level of adolescents.


Assuntos
Uso do Telefone Celular , Letramento em Saúde , Adolescente , Humanos , Masculino , Feminino , Fatores Sexuais , Inquéritos e Questionários , Estudantes
3.
Anal Chim Acta ; 1243: 340809, 2023 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-36697175

RESUMO

Tricresyl phosphate (TCP), a notable emerging pollutant with a high bioconcentration factor and biotoxicity, is a typical representative of aryl-organophosphorus flame retardants. The electrochemical and chromatographic technologies used in conventional TCP detection have a variety of drawbacks. Hence, it is crucial to suggest an easy, accurate, and selective method for detecting TCP. In this study, we presented a brand-new method based on NH2-MIL-53(Al) nanoprobe for the direct luminescence assay of TCP. NH2-MIL-53(Al) possessed an excellent crystal structure and superior optical qualities. Notably, the introduction of TCP caused a considerable dampening of the photoluminescence signal of the nanoprobe. The fluorescence response based on static quenching was verified by fluorescence lifetime decay curves. The thermodynamic analysis further concluded that TCP and nanoprobe spontaneously produced non-fluorescent complexes due to hydrophobic interaction. The quenching efficiency (F0-F)/F0 of the nanoprobe and the TCP concentration displayed good linearity in the scope of 0.3-3.0 µM (R2 = 0.996), and the LOD was 0.058 µM under the ideal detection conditions. More significantly, the technique was effectively used to identify TCP in lake and tap water (RSD ≤5.79%), which provided a fresh perspective on how to recognize OPFRs in environmental water.

4.
Int J Ophthalmol ; 16(1): 33-39, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36659935

RESUMO

AIM: To investigate the anti-angiogenic effect of apolipoprotein A1 (apoA1) on primary human retinal vascular endothelial cells (HRECs) and explore the possible mechanism. METHODS: The primary HRECs were transfected with apoA1-GFP recombinant lentiviral and were compared with cells undergoing transfection with empty lentiviral vectors. Hypoxia chambers were used to simulate the anoxic environment of cells under pathological condition. The concentrations of secreted vascular endothelial growth factor (VEGF) and placental growth factor (PlGF) were measured by enzyme-linked immunosorbent assay (ELISA). Cell migration ability was detected by wound healing assay. The sprouting of HRECs was determined by tube formation assay. The protein levels of extracellular signal regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 (p-ERK1/2) were measured by Western blot. RESULTS: Overexpressed apoA1 in hypoxia-induced HRECs significantly suppressed PlGF (0.67±0.10 folds, P=0.007). Overexpressed apoA1 also attenuated hypoxia-induced cell migration (0.32±0.11 folds, P<0.0001), tube formation (0.66±0.01 folds, P<0.0001) and the phosphorylation levels of ERK (0.6±0.11 folds, P=0.025). Pretreatment of mitogen-activated protein kinase kinase (MEK) inhibitor (U0126) further reduced the PlGF and angiogenesis in hypoxia-induced HRECs. CONCLUSION: ApoA1 inhibits the angiogenesis at least in part by inactivating ERK1/2 in hypoxia-induced HRECs. Moreover, apoA1 suppresses the PlGF expression, which selectively associated with pathological angiogenesis.

5.
Lett Appl Microbiol ; 76(1)2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36688785

RESUMO

The efficacy of Lactobacillus as an antibiotic substitute has been investigated as one of the potential strategies to prevent Salmonella infection in poultry. The purpose of this study was to explore the antibacterial activity of Lactobacillus fermentum 1.2133 (Lact. fermentum 1.2133) against Salmonella pullorum CVCC533 (Salm. pullorum CVCC533) and its effect on chickens infected with Salm. pullorum CVCC533. Results showed that Lact. fermentans 1.2133 has antibacterial activity against Salm. pullorum CVCC533 and the cell-free fermentation supernatant of Lact. fermentum 1.2133 had a bactericidal effect on the bacteria in the Salm. pullorum CVCC533 biofilm by significantly reducing the number of Salmonella and aerobic bacteria in the chicken duodenum, ileum, and cecum, including Escherichia shigella (P < 0.05), improved the species abundance of Lactobacilli (P < 0.05). The damage to the chicken intestine by Salm. pullorum CVCC533 was reduced as the expression of avian beta-defensin 2 (AvBD2) mRNA in chicken small intestine was increased (P < 0.05). The results showed that Lact. fermentum 1.2133 had the potential to be a probiotic for poultry due to its regulation of intestinal AvBD2 mRNA as well as its intestinal flora.


Assuntos
Doenças das Aves Domésticas , Probióticos , Salmonelose Animal , Animais , Galinhas/microbiologia , Lactobacillus/fisiologia , Salmonella , Aves Domésticas , Antibacterianos
6.
Mater Today Bio ; 18: 100546, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36691606

RESUMO

Nanoparticle technologies offer a non-invasive means to deliver basic fibroblast growth factor (bFGF) for the treatment of spinal cord injury (SCI). However, the inability of bFGF to accumulate at the injury site and inefficient penetration across the blood-spinal cord barrier (BSCB) remain challenges. The present study describes a dual-targeting liposome (bFGF@Lip-Cp&Rp) with injury lesion targeting and BSCB-penetrating capability to deliver bFGF for SCI treatment. The CAQK peptide (Cp) with injury lesion targeting ability and R2KC peptide (Rp) with BSCB-penetrating capability were grafted onto the liposomes for a flexible and non-invasive drug delivery systems preparation. Results exhibit that the dual-targeted liposomes could significantly cross the BSCB and accumulate at the injury site. During the early stage of SCI, bFGF@Lip-Cp&Rp promotes repair of BSCB and facilitates M2-polarization of macrophages. Regular delivery of bFGF@Lip-Cp&Rp increase HUVECs tube formation and angiogenesis, ameliorate the microenvironment of lesion site, suppress the neuronal apoptosis and axonal atrophy in SCI rats. Importantly, continuous treatment of bFGF@Lip-Cp&Rp supports the restoration of limb motor function in SCI rats. In summary, this research implies that the injury site-targeting and BSCB-penetrating liposomes could be a promising therapeutic approach for the treatment of SCI.

7.
Materials (Basel) ; 16(2)2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36676437

RESUMO

Both the discrete and continuous particle packing models are used to design UHPC, but the influences of a water film covering the particle surfaces on the compactness of the particle system were not considered in these models. In fact, the water film results in a certain distance between solid particles (DSP), which affects the compactness of the particle system, especially for cementitious materials with small particle sizes. In the present study, the mixture design method for UHPC was proposed based on the Fuller distribution model modified using the DSP. Then, the components of cementitious materials and aggregates were optimized, and the UHPC matrices with high solid concentrations were obtained. The results showed that the solid concentration, slump flow, and compressive strength of the UHPC matrix reached 77.1 vol.%, 810 mm, and 162.0 MPa, respectively. By replacing granulated blast furnace slag (GBFS) with quartz powder (QP), the flexural strength of the UHPC matrix was increased without reducing its compressive strength. When the steel fiber with a volume fraction of 1.5% was used, the slump flow, compressive strength, tensile strength, and flexural strength of the UHPC reached 740 mm, 175.6 MPa, 9.7 MPa, and 22.8 MPa, respectively. After 500 freeze-thaw cycles or 60 dry-wet cycles under sulfate erosion, the mechanical properties did not deteriorate. The chloride diffusion coefficients in UHPCs were lower than 3.0 × 10-14 m2/s, and the carbonation depth of each UHPC was 0 mm after carbonization for 28 days. The UHPCs presented ideal workability, mechanical properties, and durability, demonstrating the validity of the method proposed for UHPC design.

8.
Pharm Biol ; 61(1): 155-164, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36604840

RESUMO

CONTEXT: Salvia miltiorrhizae Bunge (Lamiaceae) is a traditional Chinese medicine (TCM) for the treatment of 'thoracic obstruction'. Transient receptor potential canonical channel 1 (TRPC1) is a important target for myocardial injury treatment. OBJECTIVE: This work screens the active component acting on TRPC1 from Salvia miltiorrhizae. MATERIALS AND METHODS: TCM Systems Pharmacology Database and Analysis Platform (TCMSP) was used to retrieve Salvia miltiorrhiza compounds for preliminary screening by referring to Lipinski's rule of five. Then, the compound group was comprehensively scored by AutoDock Vina based on TRPC1 protein. Surface plasmon resonance (SPR) was used to determine the affinity of the optimal compound to TRPC1 protein. Western blot assay was carried out to observe the effect of the optimal compound on TRPC1 protein expression in HL-1 cells, and Fura-2/AM detection was carried out to observe the effect of the optimal compound on calcium influx in HEK293 cells. RESULTS: Twenty compounds with relatively good characteristic parameters were determined from 202 compounds of Salvia miltiorrhiza. Rosmarinic acid (RosA) was obtained based on the molecular docking scoring function. RosA had a high binding affinity to TRPC1 protein (KD value = 1.27 µM). RosA (50 µM) could reduce the protein levels (417.1%) of TRPC1 after oxygen-glucose deprivation/reperfusion (OGD/R) in HL-1 cells and it could inhibit TRPC1-mediated Ca2+ influx injury (0.07 ΔRatio340/380) in HEK293 cells. DISCUSSION AND CONCLUSIONS: We obtained the potential active component RosA acting on TRPC1 from Salvia miltiorrhizae, and we speculate that RosA may be a promising clinical candidate for myocardial injury therapy.


Assuntos
Salvia miltiorrhiza , Humanos , Salvia miltiorrhiza/química , Simulação de Acoplamento Molecular , Células HEK293 , Cinamatos/farmacologia
9.
Int J Biol Macromol ; 230: 123168, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36621734

RESUMO

Cellulose nanocrystals (CNCs), as the promising reinforcing fillers in the rubber industry, their surface chemical groups have vital effects on the vulcanization kinetics, cross-linking densities, and mechanical properties of rubber composites. Herein, CNCs with acidic carboxyl (CCA) and alkaline amino groups (CCP) were produced by modifying the sulfonic CNCs (CCS) in environment-friendly ways. Studies found the CCS and CCA with acid groups have obvious inhibiting effects on the vulcanization of natural rubber (NR), while CCP with alkaline amino groups accelerates the vulcanization of NR. Differential scanning calorimeter, Fourier transform infrared spectroscopy, and Electron paramagnetic resonance, etc. were performed to clarify the effecting mechanisms of CNCs surface groups on NR vulcanization. It was found that NR/CCS and NR/CCA nanocomposites vulcanize through radical reactions, and the surface acidic groups of CCS and CCA, i.e., hydroxyl, sulfonate, and carboxyl groups inactivate the sulfur radicals generated during vulcanization and depress the vulcanization activity. The amino groups of the polyethyleneimine of CCP promote the ring opening of sulfur (S8) or the breaking of polysulfide bonds connected to NR molecular chains to form sulfur anion with a strong nucleophilic ability, which leads to the cross-linking of NR/CCP reacts via ionic reaction mainly. The vulcanization rate and cross-linking density of NR/CCP are improved by the ionic reaction. And benefiting from the higher cross-linking density and the reinforcement of CCP, NR/CCP had the best physical and mechanical properties. Our work elucidates the mechanism of the surface chemical groups of CNCs affecting NR vulcanization and may provide ideas for the preparation of high-performance rubber composites reinforced by CNCs.

10.
CNS Neurosci Ther ; 29(2): 609-618, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36480481

RESUMO

AIMS: Alterations in neuronal activity and cerebral hemodynamics have been reported in idiopathic generalized epilepsy (IGE) patients, possibly resulting in neurovascular decoupling; however, no neuroimaging evidence confirmed this disruption. This study aimed to investigate the possible presence of neurovascular decoupling and its clinical implications in childhood IGE using resting-state fMRI and arterial spin labeling imaging. METHODS: IGE patients and healthy participants underwent resting-state fMRI and arterial spin labeling imaging to calculate degree centrality (DC) and cerebral blood flow (CBF), respectively. Across-voxel CBF-DC correlations were analyzed to evaluate the neurovascular coupling within the whole gray matter, and the regional coupling of brain region was assessed with the CBF/DC ratio. RESULTS: The study included 26 children with IGE and 35 sex- and age-matched healthy controls (HCs). Compared with the HCs, the IGE group presented lower across-voxel CBF-DC correlations, higher CBF/DC ratio in the right posterior cingulate cortex/precuneus, middle frontal gyrus, and medial frontal gyrus (MFG), and lower ratio in the left inferior frontal gyrus. The increased CBF/DC ratio in the right MFG was correlated with lower performance intelligence quotient scores in the IGE group. CONCLUSION: Children with IGE present altered neurovascular coupling, associated with lower performance intelligence quotient scores. The study shed a new insight into the pathophysiology of epilepsy and provided potential imaging biomarkers of cognitive performances in children with IGE.


Assuntos
Epilepsia Generalizada , Acoplamento Neurovascular , Humanos , Criança , Acoplamento Neurovascular/fisiologia , Epilepsia Generalizada/diagnóstico por imagem , Encéfalo/diagnóstico por imagem , Imageamento por Ressonância Magnética , Marcadores de Spin , Imunoglobulina E
11.
Curr Microbiol ; 80(1): 14, 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36459213

RESUMO

Mycoplasma pneumoniae, an obligate parasitic pathogen without cell wall, can cause severe upper and lower respiratory tract symptoms. It is the pathogen of human bronchitis and walking pneumonia, and named community-acquired pneumonia. In addition to severe respiratory symptoms, there are clinical extrapulmonary manifestations in the skin, brain, kidney, musculoskeletal, digestive system, and even blood system after M. pneumoniae infection. Hereby, we comprehensively summarized and reviewed the intrapulmonary and extrapulmonary pathogenesis of M. pneumoniae infection. The pathogenesis of related respiratory symptoms caused by M. pneumoniae is mainly adhesion damage, direct damage including nutrient predation, invasion and toxin, cytokine induced inflammation damage and immune evasion effect. The pathogenesis of extrapulmonary manifestations includes direct damage mediated by invasion and inflammatory factors, indirect damage caused by host immune response, and vascular occlusion. The intrapulmonary and extrapulmonary pathogenic mechanisms of M. pneumoniae infection are independent and interrelated, and have certain commonalities. In fact, the pathogenic mechanisms of M. pneumoniae are complicated, and the specific content is still not completely clear, further researches are necessary for determining the detailed pathogenesis of M. pneumoniae. This review can provide certain guidance for the effective prevention and treatment of M. pneumoniae infection.


Assuntos
Parede Celular , Mycoplasma pneumoniae , Humanos , Citocinas , Inflamação , Rim
12.
Redox Biol ; 58: 102558, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36462232

RESUMO

Tyrosine-protein phosphatase non-receptor type 1 (Ptpn1) is known to be involved in macrophage polarization. However, whether and how Ptpn1 regulates macrophage phenotype to affect intestinal epithelial barrier function remains largely unexplored. Herein, we investigated the impact of Ptpn1 and macrophage-derived small extracellular vesicles (sEVs) on macrophage-intestinal epithelial cell (IEC) interactions in the context of intestinal inflammation. We found that Ptpn1 knockdown shifts macrophages toward the anti-inflammatory M2 phenotype, thereby promoting intestinal barrier integrity and suppressing inflammatory response in the macrophage-IEC co-culture model. We further revealed that conditioned medium or sEVs isolated from Ptp1b knockdown macrophages are the primary factor driving the beneficial outcomes. Consistently, administration of the sEVs from Ptpn1-knockdown macrophages reduced disease severity and ameliorated intestinal inflammation in LPS-challenged mice. Furthermore, depletion of macrophages in mice abrogated the protective effect of Ptpn1-knockdown macrophage sEVs against Salmonella Typhimurium infection. Importantly, we found lactadherin to be highly enriched in the sEVs of Ptpn1-knockdown macrophages. Administration of recombinant lactadherin alleviated intestinal inflammation and barrier dysfunction by inducing macrophage M2 polarization. Interestingly, sEVs lactadherin was also internalized by macrophages and IECs, leading to macrophage M2 polarization and enhanced intestinal barrier integrity. Mechanistically, the anti-inflammatory and barrier-enhancing effect of lactadherin was achieved by reducing TNF-α and NF-κB activation. Thus, we demonstrated that sEVs from Ptpn1-knockdown macrophages mediate the communication between IECs and macrophages through enrichment of lactadherin. The outcome could potentially lead to the development of novel therapies for intestinal inflammatory disorders.


Assuntos
Vesículas Extracelulares , Macrófagos , Animais , Camundongos , Proteína Fosfatase 1 , Anti-Inflamatórios/farmacologia , Inflamação/genética
13.
World J Surg Oncol ; 20(1): 377, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36451200

RESUMO

BACKGROUND: Hepatocellular carcinoma (HCC) is still fatal even after surgical resection. The purpose of this study was to analyze the prognostic factors of 5-year survival rate and to establish a model to identify HCC patients with gain of surgery combined with chemotherapy. METHODS: All patients with HCC after surgery from January 2010 to December 2015 were selected from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate logistic analysis were used to analyze the prognostic factors of patients, and the risk prediction model of 5-year survival rate of HCC patients was established by classical decision tree method. Propensity score matching was used to eliminate the confounding factors of whether to receive chemotherapy in high-risk group or low-risk group. RESULTS: One-thousand six-hundred twenty-five eligible HCC patients were included in the study. Marital status, α-fetoprotein (AFP), vascular infiltration, tumor size, number of lesions, and grade were independent prognostic factors affecting the 5-year survival rate of HCC patients. The area under the curve of the 5-year survival risk prediction model constructed from the above variables was 0.76, and the classification accuracy, precision, recall, and F1 scores were 0.752, 0.83, 0.842, and 0.836, respectively. High-risk patients classified according to the prediction model had better 5-year survival rate after chemotherapy, while there was no difference in 5-year survival rate between patients receiving chemotherapy and patients not receiving chemotherapy in the low-risk group. CONCLUSIONS: The 5-year survival risk prediction model constructed in this study provides accurate survival prediction information. The high-risk patients determined according to the prediction model may benefit from the 5-year survival rate after combined chemotherapy.


Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/cirurgia , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/cirurgia , Aprendizado de Máquina , Pontuação de Propensão , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
14.
Bull Entomol Res ; : 1-10, 2022 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-36503531

RESUMO

Liriomyza trifolii is a significant pest of vegetable and ornamental crops across the globe. Microwave radiation has been used for controlling pests in stored products; however, there are few reports on the use of microwaves for eradicating agricultural pests such as L. trifolii, and its effects on pests at the molecular level is unclear. In this study, we show that microwave radiation inhibited the emergence of L. trifolii pupae. Transcriptomic studies of L. trifolii indicated significant enrichment of differentially expressed genes (DEGs) in 'post-translational modification, protein turnover, chaperones', 'sensory perception of pain/transcription repressor complex/zinc ion binding' and 'insulin signaling pathway' when analyzed with the Clusters of Orthologous Groups, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases, respectively. The top DEGs were related to reproduction, immunity and development and were significantly expressed after microwave radiation. Interestingly, there was no significant difference in the expression of genes encoding heat shock proteins or antioxidant enzymes in L. trifolii treated with microwave radiation as compared to the untreated control. The expression of DEGs encoding cuticular protein and protein takeout were silenced by RNA interference, and the results showed that knockdown of these two DEGs reduced the survival of L. trifolii exposed to microwave radiation. The results of this study help elucidate the molecular response of L. trifolii exposed to microwave radiation and provide novel ideas for control.

15.
J Family Med Prim Care ; 11(9): 5527-5534, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36505657

RESUMO

Aims: This study aimed to construct a standardized health risk assessment index system (HRAIS) under the guidance of general health and facilitate the family doctors to carry out chronic disease management. Methods: Available chronic disease surveillance systems and indexes were reviewed to identify potential indexes. The Delphi method was performed to establish the HRAIS, and the analytic hierarchy process was used to calculate the index weight. Results: HRAIS included four first-level indexes and 38 second-level indexes. The authority coefficient was 0.86. The Kendall's W for the two rounds of Delphi consultation were 0.202 and 0.210 (p < 0.001). The weights of the first-level indexes from high to low were physiological health (0.409), psychosocial health (0.290), health-related behaviors (0.205), and environment (0.097). Thus, HRAIS is a multi-dimension and multi-index tool, which can be used as a guideline for family doctors in early screening, early intervention, and classified management of main chronic diseases.

16.
RSC Adv ; 12(52): 33716-33724, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36505694

RESUMO

The mixed bimetal metal-organic framework Ni0.37Co0.63-MOF-74 has been constructed by the solvothermal method for NO adsorption. The results showed that bimetal Ni0.37Co0.63-MOF-74 takes up NO with a capacity of up to 174.3 cc g-1 under ambient conditions, which is 16.3% higher than that of the best single metal Co-MOF-74. The IAST adsorption selectivity for a NO/CO2 binary mixture can reach a maximum of 710 at low adsorption partial pressure, while the regeneration performance can be retained even after five cyclic adsorption-desorption experiments. Its separation performance was further confirmed by breakthrough experiments, indicating this new bimetal Ni0.37Co0.63-MOF-74 as one of the best materials for NO adsorption and separation in flue gas.

17.
Int J Nanomedicine ; 17: 5915-5931, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36506343

RESUMO

Introduction: Glaucoma is a chronic disease that requires long-term adherence to treatment. Topical application of conventional eye drops results in substantial drug loss due to rapid tear turnover, with poor drug bioavailability being a major challenge for efficient glaucoma treatment. We aimed to prepare the anti-glaucoma drug betaxolol hydrochloride (BH) as a novel nano-delivery system that prolonged the retention time at the ocular surface and improved bioavailability. Methods: We constructed multifunctional nanoparticles (MMt-BH-HA/CS-ED NPs) by ion cross-linking-solvent evaporation method. The particle size, zeta potential, encapsulation efficiency and drug loading of MMt-BH-HA/CS-ED NPs were physicochemically characterized. The structure of the preparations was characterized by microscopic techniques of SEM, TEM, XPS, XRD, FTIR and TGA, and evaluated for their in vitro release performance as well as adhesion properties. Its safety was investigated using irritation assays of hemolysis experiment, Draize test and histopathology examination. Precorneal retention was examined by in vivo fluorescence tracer method and pharmacokinetics in tear fluid was studied. A model of high IOP successfully induced by injection of compound carbomer solution was used to assess the IOP-lowering efficacy of the formulation, and it was proposed that micro-interactions between the formulation and the tear film would be used to analyze the behavior at the ocular surface. Results: The positively charged MMt-BH-HA/CS-ED NPs were successfully prepared with good two-step release properties, higher viscosity, and slower pre-corneal diffusion rate along with longer precorneal retention time compared to BH solution. The micro-interactions between nanoparticles and tear film converted the drug clearance from being controlled by fast aqueous layer turnover to slow mucin layer turnover, resulting in higher drug concentration on the ocular surface, providing more durable and stable IOP-lowering efficacy. Conclusion: The novel multifunctional MMt-BH-HA/CS-ED NPs can effectively reduce IOP and are suitable for the treatment of chronic disease glaucoma.


Assuntos
Glaucoma , Nanopartículas , Humanos , Betaxolol , Pressão Intraocular , Nanopartículas/química , Glaucoma/patologia , Córnea , Tamanho da Partícula , Portadores de Fármacos/química
18.
Heliyon ; 8(12): e12086, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36561663

RESUMO

Pedestrian attribute recognition (PAR) and re-identification (ReID) are important works in the area of computer vision, which are widely used in intelligent surveillance and are of great significance to the creation of smart life. The purpose of this article is to focus on organizing a review of ReID based on deep learning and analyze the associations between PAR and ReID. Firstly, we summarize the major ideas of Attribute-Assisted ReID and compare the differences in datasets and algorithmic concerns between the two areas. Secondly, we introduce a wide range of representative ReID methods. By analyzing some cutting-edge researches, we summarize their specific network structure, loss function design, and effective training tricks. Reference methods and solutions are provided for the main challenges of ReID, such as cloth-changing, domain adaptation, occlusion condition, resolution changes, etc. Finally, we conclude the performance and characteristics of the SOTA methods, obtain inspiration and prospects for future research directions, and demonstrate the effectiveness of Attribute-Assisted ReID.

19.
Front Neurol ; 13: 1072351, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36570456

RESUMO

Objective: Hypoxia-inducible factor 1alpha (HIF-1α) functions as a crucial transcriptional mediator in hypoxic and ischemic brain response. We endeavored to assess the prognostic significance of serum HIF-1α in human aneurysmal subarachnoid hemorrhage (aSAH). Methods: In this prospective, longitudinal, multicenter, and observational study of 257 patients with aSAH and 100 healthy controls, serum HIF-1α levels were quantified. Univariate analyses, followed by multivariate analyses, were performed to discern the relationship between serum HIF-1α levels and severity and delayed cerebral ischemia (DCI) plus poststroke 6-month poor outcome [extended Glasgow outcome scale (GOSE) scores of 1-4]. Predictive efficiency was determined under the receiver operating characteristic (ROC) curve. Results: There were significantly increased serum HIF-lα levels after aSAH, in comparison to controls (median, 288.0 vs. 102.6 pg/ml; P < 0.001). Serum HIF-lα levels were independently correlated with Hunt-Hess scores [ß, 78.376; 95% confidence interval (CI): 56.446-100.305; P = 0.001] and modified Fisher scores (ß, 52.037; 95% CI: 23.461-80.614; P = 0.002). Serum HIF-lα levels displayed significant efficiency for discriminating DCI risk [area under ROC curve (AUC), 0.751; 95% CI: 0.687-0.815; P < 0.001] and poor outcome (AUC, 0.791; 95% CI: 0.736-0.846; P < 0.001). Using the Youden method, serum HIF-1α levels >229.3 pg/ml predicted the development of DCI with 92.3% sensitivity and 48.4% specificity and serum HIF-1α levels >384.0 pg/ml differentiated the risk of a poor prognosis with 71.4% sensitivity and 81.1% specificity. Serum HIF-1α levels >229.3 pg/ml were independently predictive of DCI [odds ratio (OR), 3.061; 95% CI: 1.045-8.965; P = 0.041] and serum HIF-1α levels >384.0 pg/ml were independently associated with a poor outcome (OR, 2.907; 95% CI: 1.403-6.024; P = 0.004). The DCI predictive ability of their combination was significantly superior to those of Hunt-Hess scores (AUC, 0.800; 95% CI: 0.745-0.855; P = 0.039) and modified Fisher scores (AUC, 0.784; 95% CI: 0.726-0.843; P = 0.004). The prognostic predictive ability of their combination substantially exceeded those of Hunt-Hess scores (AUC, 0.839; 95% CI: 0.791-0.886; P < 0.001) and modified Fisher scores (AUC, 0.844; 95% CI: 0.799-0.890; P < 0.001). Conclusion: Elevated serum HIF-lα levels after aSAH, in independent correlation with stroke severity, were independently associated with DCI and 6-month poor outcome, substantializing serum HIF-lα as a potential prognostic biomarker of aSAH.

20.
J Arthroplasty ; 2022 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-36529195

RESUMO

BACKGROUND: This study examined whether pericapsular nerve group (PENG) block combined with local infiltration analgesia (LIA) could improve pain management and functional recovery after total hip arthroplasty. METHODS: All patients were randomly assigned to receive PENG block combined with LIA (PENG group) or sham PENG block and LIA (Sham group). The primary outcome was cumulative morphine consumption within 24 hours after surgery. Secondary outcomes were pain scores on a visual analog scale (VAS); time to first rescue analgesia; cumulative morphine consumption during hospitalization; intraoperative consumption of opioids; postoperative recovery; and postoperative complications. RESULTS: PENG patients consumed significantly less morphine within the first 24 hours and throughout hospitalization and smaller amounts of intraoperative opioids. There were significantly lower pain scores at rest and during motion within 24 hours in PENG patients. PENG patients took significantly longer until the first rescue analgesia and showed significantly better postoperative rehabilitation. However, the absolute change in morphine consumption and VAS scores did not exceed the reported minimal clinically important differences (morphine consumption: 10 mg; VAS scores: 1.5 at rest and 1.8 during movement). The two groups showed no difference in quadriceps muscle strength and postoperative complications. CONCLUSION: PENG block combined with LIA could improve postoperative pain relief, reduce opioid use, and enhance recovery in total hip arthroplasty patients, without weakening the quadriceps muscle strength. This work justifies further trials to examine the safety and efficacy of this block and to explore maximal effective volume of local anesthetic for motor-sparing PENG block.

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