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1.
Environ Pollut ; 259: 113849, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31931410

RESUMO

The present study evaluated the feasibility of using a marine cage fish Larimichthys crocea as a model for monitoring short-time Cd discharge near the sewage outlet. Fish were exposed to 0, 20, 100, 500 and 2500 µg/L for 6 h. Cd concentrations in gills, and left and right lobes of hepatopancreas were examined as well as activity levels of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathion-S-transferase (GST), glutathione reductase (GR), lipid peroxidation, glutathione (GSH) and mRNA levels of 19 genes encoding these enzymes. Cd concentrations increased at 100, 500 and 2500 µg/L Cd in gill and at 2500 µg/L Cd in hepatopancreas. Lipid peroxidation increased and GSH levels declined in gills at 2500 µg/L Cd. On the contrary, oxidative damage was not observed in hepatopancreas but GSH levels increased at all tested concentrations of Cd in the left lobe and at 20 µg/L Cd in the right lobe. The enhanced antioxidant response was confirmed in gills due to the increased activity levels of antioxidant enzymes and the up-regulated mRNA levels of most genes. However, disordered antioxidant response was observed in hepatopancreas, showing a dose- and lobe-dependent effect. RNA-seq and q-PCR analyses were performed to investigate differently expressed genes between both lobes under different concentrations of Cd. The most significantly enriched pathway term was pancreatic secretion, where the right lobe showed higher mRNA levels of 18 genes encoding pancreatic digestive enzymes than the left one under Cd stress. Interestingly, both lobes had the same mRNA levels of digestive enzyme genes and antioxidant genes in fish without Cd exposure. Overall, Larimichthys crocea is very sensitive to environmental exposure to cadmium. The present study for the first time investigates Cd-induced antioxidant response in Larimichthys crocea, also is the first to find lobe-dependent effects in fish.

2.
Neuropsychobiology ; : 1-10, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31940619

RESUMO

BACKGROUND: Depression is one of the most common neuropsychiatric disturbances in Parkinson's disease (PD), but its pathophysiology is not definite. Lines of evidence have indicated that the hippocampus and serotonin 1A (5-HT1A) receptors are related to the regulation of depression. OBJECTIVE: The purpose of the present study was to observe the effect of 5-HT1A receptors in the dorsal hippocampus (dHIP) on PD-related depression in rats. METHODS: Unilateral 6-hydroxydopamine lesioning of the medial forebrain bundle (MFB) was used to establish the hemiparkinsonian rat model. The effects of intra-dHIP injection of the 5-HT1A receptor -agonist 8-hydroxy-2-(dipropylamino)tetralin hydrobromide (8-OH-DPAT) or antagonist WAY-100635 on depressive-like behaviors were observed in sucrose preference and forced swim tests in control and lesioned rats. Monoamine levels including dopamine (DA), 5-HT, and noradrenaline (NA) in depression-related brain regions were determined by a neurochemical method in all groups. RESULTS: Behavioral results showed that MFB lesions induced depressive-like behaviors. Intra-dHIP injection of 8-OH-DPAT produced antidepressant effects, while WAY-100635 induced or increased the depressive-like behaviors in both control and the lesioned rats. Neurochemical results found that intra-dHIP injection of 8-OH-DPAT significantly increased DA and 5-HT levels in the medial prefrontal cortex (mPFC), lateral habenula (LHb), ventral hippocampus and amygdala in the lesioned group and decreased NA levels in the mPFC and LHb in the control group. Moreover, after injection of WAY-100635, NA levels in all these regions of the lesioned group were significantly increased. CONCLUSIONS: These findings suggest that hippocampal 5-HT1A receptors regulate depression and PD-related depression by neurochemical mechanisms.

3.
Aquat Toxicol ; 220: 105408, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31935571

RESUMO

Tributyltin (TBT), an aromatase inhibitor, has been found to disrupt gametogenesis and reproductive behavior in several fish species. However, whether TBT is capable of affecting other behaviors such as aggressive behavior and fear response in fish and the underlying mode(s) of action remain unclear. To study aggressive behavior, adult zebrafish (Danio rerio) males were continuously exposed to two nominal concentrations of TBT (TBT-low, 100 ng/L and TBT-high, 500 ng/L) for 28 days. To study the fear response, the fish were divided into four groups (Blank and Control, 0 ng/L TBT; TBT-low, 100 ng/L; and TBT-high, 500 ng/L). The fish were then treated with DW (Blank) or with alarm substance (AS) (Control, TBT-low and TBT-high). After exposure, the aggressive behavior of the fish was tested using the mirror test (mirror-biting frequency, approaches to the mirror and duration in approach zone).and fighting test (fish-biting frequency) The mirror-biting frequency, approaches to the mirror, duration in approach zone and fish-biting frequency of the TBT-exposed fish increased significantly compared to those of the control fish, indicating enhanced aggressive behavior. The fear response parameters tested using the novel tank dive test (onset time to the higher half, total duration in the lower half and the frequency of turning) of the TBT-exposed fish were also significantly increased after AS administration, suggesting an enhanced fear response. Further investigation revealed that TBT treatment elevated the plasma level of 11-ketotestosterone (11-KT) and decreased the plasma level of estradiol (E2) in a concentration-dependent manner. Moreover, TBT up-regulated the mRNA levels of ar, c-fos and bdnf1, and suppressed the expression of btg-2 in fish. In addition, exposure to AS increased the plasma level of cortisol and down-regulated the mRNA expression levels of genes involved in 5-HT synthesis (such as tph1b and pet1) in both control and TBT-treated fish. AS significantly suppressed the mRNA level of tph1b, tph2, pet1 and npy in the TBT-high group compared to the control fish. The present study demonstrates that TBT enhances aggressive behavior and fear responses in male zebrafish probably through altering plasma levels of 11-KT, E2 and cortisol and altering the expression of genes involved in the regulation of aggressive behavior (ar, c-fos, bdnf1 and btg-2) and fear responses (tph1b, tph2, pet1 and npy). The present study greatly extends our understanding of the behavioral toxicity of TBT to fish.

4.
PLoS One ; 15(1): e0226972, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31914160

RESUMO

Mounting evidence indicates that coffee, a commonly consumed beverage worldwide, is inversely associated with various chronic diseases and overall mortality. Few studies have evaluated the effect of coffee drinking on telomere length, a biomarker of chromosomal integrity, and results have been inconsistent. Understanding this association may provide mechanistic insight into associations of coffee with health. The aim of our study was to test the hypothesis that heavier coffee intake is associated with greater likelihood of having above-median telomere length. We evaluated the cross-sectional association between coffee intake and relative telomere length using data from 1,638 controls from four previously conducted case-control studies nested in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Coffee intake was assessed using a food frequency questionnaire, and relative telomere length was measured from buffy-coat, blood, or buccal cells. We used unconditional logistic regression models to generate multivariable-adjusted, study-specific odds ratios for the association between coffee intake and relative telomere length. We then conducted a random-effects meta-analysis to determine summary odds ratios. We found that neither summary continuous (OR = 1.01, 95% CI = 0.99-1.03) nor categorical (OR <3 cups/day vs. none = 1.37, 95% CI = 0.71-2.65; OR ≥3 cups/day vs. none = 1.47, 95% CI = 0.81-2.66) odds ratio estimates of coffee drinking and relative telomere length were statistically significant. However, in the largest of the four contributing studies, moderate (<3 cups/day) and heavy coffee drinkers (≥3 cups/day) were 2.10 times (95% CI = 1.25, 3.54) and 1.93 times as likely (95% CI = 1.17, 3.18) as nondrinkers to have above-median telomere length, respectively. In conclusion, we found no evidence that coffee drinking is associated with telomere length. Thus, it is unlikely that telomere length plays a role in potential coffee-disease associations.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31919586

RESUMO

Microorganisms are indispensable in the food industry, but wild-type strains hardly meet the current industrial demands due to several undesirable traits. Therefore, microbial strain improvement offers a critical solution to enhance the food industry. Traditional techniques for food microbial improvement, such as the use of chemical mutagens and manual isolation/purification, are inefficient, time-consuming, and laborious, restricting further progress in the area of food fermentation. In this review, the applications of novel mutagenesis and screening technologies used for the improvement of food microbes were summarized, including random mutagenesis based on physical irradiation, microbial screening facilitated by a microtiter plate, fluorescence-activated cell or droplet sorting, and microscaled fermentation in a microtiter plate or microbioreactor. In comparison with conventional methods, these new tools have the potential in accelerating microbial strain improvement and their combined applications could create a new trend for strain development. However, several problems that could affect its potential application may include the following: the lack of specific mutagenesis devices and biosensing systems, the insufficient improvement of the mixed culture system, the low efficiency when using filamentous fungi and flocculating bacteria, and the insufficient safety assessment on harnessing genome-editing technology. Therefore, future works on strain improvement remain challenging for the food industry.

6.
J Chem Theory Comput ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31899646

RESUMO

We present an efficient way to compute the excitation energies in molecules and solids within linear-response time-dependent density functional theory (LR-TDDFT). Conventional methods to construct and diagonalize the LR-TDDFT Hamiltonian require ultrahigh computational cost, limiting its optoelectronic applications to small systems. Our new method is based on the interpolative separable density fitting (ISDF) decomposition combined with implicitly constructing and iteratively diagonalizing the LR-TDDFT Hamiltonian and only requires low computational cost to accelerate the LR-TDDFT calculations in the plane-wave basis sets under the periodic boundary condition. We show that this method accurately reproduces excitation energies in a fullerene (C60) molecule and bulk silicon Si64 system with significantly reduced computational cost compared to conventional direct and iterative calculations. The efficiency of this ISDF method enables us to investigate the excited-state properties of liquid water absorption on MoS2 and phosphorene by using the LR-TDDFT calculations. Our computational results show that an aqueous environment has a weak effect on low excitation energies but a strong effect on high excitation energies of 2D semiconductors for photocatalytic water splitting.

7.
Huan Jing Ke Xue ; 41(1): 139-145, 2020 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854914

RESUMO

Chinese emission standard of air pollutants for bulk gasoline terminals (GB 20950-2007) stipulate standards for vapor emissions during gasoline storage and receiving in bulk gasoline terminals. However, the standards are not applicable to crude oil, aviation kerosene, naphtha, and other kinds of oil. We assess emission standards or directives for vapor processing equipment in terminals in the United States (US) and European Union (EU), and analyze the emission status of vapor processing equipment in three typical cities in China. We further propose revisions to GB 20950-2007. We made the following observations. ① US and EU standards include scope not only for gasoline, but also crude oil and other organic liquids. ② The emission limits of non-methane hydrocarbons defined in GB 20950-2007 are i) 0.5, 1.8, and 8.9 times those defined in Subpart XX, Subpart R, and Subpart Y in the US federal regulations, ii) 1.8 and 3.1 times those defined in Rule 462 and Rule 1142 in southern California law, and iii) 0.7 and 500 times those defined in EU and German directives, respectively. The vapor leakage limit for general areas of China is 0.5 times that defined in Subpart XX of the US standards, whereas the limits for some other specific areas of China, are 0.7 and 2.0 times those defined by Rule 462 and Rule 1142 in southern California law. ③ The numerical range of P5th-P95th of NMHC emissions from the inlet and outlet of vapor processing equipment in three typical cities of China were 115-811 g·m-3 and 0.1-20.0 g·m-3, respectively. The proportion of NMHC emission concentrations less than or equal to 10 g·m-3 at the outlet of vapor processing equipment was>85%. We suggest that the scope of application of GB 20950-2007 should be extended to crude oil, gasoline (including ethanol gasoline), aviation kerosene, and naphtha. The emission concentration limit of NMHC from vapor processing equipment should be tighten from 25 g·m-3 to 20 g·m-3, with a emission limit of 10 g·m-3 added for particular cases.

8.
Biosci Biotechnol Biochem ; 84(1): 85-94, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31794329

RESUMO

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) regulates collagen-mediated platelet activation through its cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs). However, the function of CEACAM1's extracellular cleavage fragments is currently unknown. In the present study, we used mass spectrometry (MS) to identify 9 cleavage fragments shed by matrix metallopeptidase 12 (MMP-12), and then we synthesized peptides with sequences corresponding to the fragments. QLSNGNRTLT (QLSN), a peptide from the A1-domain of CEACAM1, significantly attenuated collagen-induced platelet aggregation. QLSN also attenuated platelet static adhesion to collagen. Additionally, QLSN reduced human platelet secretion and integrin αIIbß3 activation in response to glycoprotein VI (GPVI)-selective agonist, convulxin. Correspondingly, QLSN treatment significantly decreased convulxin-mediated phosphorylation of Src, protein kinase B (Akt), spleen tyrosine kinase (Syk) and phospholipase Cγ2 (PLCγ2) in human platelets. These data indicate that the CEACAM1-derived peptide QLSN inhibits GPVI-mediated human platelet activation. QLSN could potentially be developed as a novel antiplatelet agent.


Assuntos
Antígenos CD/metabolismo , Moléculas de Adesão Celular/metabolismo , Colágeno/metabolismo , Oligopeptídeos/farmacologia , Ativação Plaquetária/efeitos dos fármacos , Glicoproteínas da Membrana de Plaquetas/metabolismo , Plaquetas/metabolismo , Adesão Celular/efeitos dos fármacos , Venenos de Crotalídeos/farmacologia , Humanos , Motivo de Inibição do Imunorreceptor Baseado em Tirosina/fisiologia , Lectinas Tipo C , Metaloproteinase 12 da Matriz/metabolismo , Oligopeptídeos/síntese química , Fosfolipase C gama/metabolismo , Fosforilação/efeitos dos fármacos , Agregação Plaquetária/efeitos dos fármacos , Glicoproteínas da Membrana de Plaquetas/agonistas , Domínios Proteicos/fisiologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Quinase Syk/metabolismo
9.
Int J Cancer ; 146(3): 839-849, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31001807

RESUMO

Specific organochlorines (OCs) have been associated with non-Hodgkin lymphoma (NHL) with varying degrees of evidence. These associations have not been evaluated in Asia, where the high exposure and historical environmental contamination of certain OC pesticides (e.g., dichlorodiphenyltrichloroethane [DDT], hexachlorocyclohexane [HCH]) are different from Western populations. We evaluated NHL risk and prediagnostic blood levels of OC pesticides/metabolites and polychlorinated biphenyl congeners in a case-control study of 167 NHL cases and 167 controls nested within three prospective cohorts in Shanghai and Singapore. Conditional logistic regression was used to analyze lipid-adjusted OC levels and NHL risk. Median levels of p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE), the primary DDT metabolite, and ß-HCH were up to 12 and 65 times higher, respectively, in samples from the Asian cohorts compared to several cohorts in the United States and Norway. An increased risk of NHL was observed among those with higher ß-HCH levels both overall (3rd vs. 1st tertile OR = 1.8, 95%CI = 1.0-3.2; ptrend = 0.049) and after excluding cases diagnosed within 2 years of blood collection (3rd vs. 1st tertile OR = 2.0, 95%CI = 1.1-3.9; ptrend = 0.03), and the association was highly consistent across the three cohorts. No significant associations were observed for other OCs, including p,p'-DDE. Our findings provide support for an association between ß-HCH blood levels and NHL risk. This is a concern because substantial quantities of persistent, toxic residues of HCH are present in the environment worldwide. Although there is some evidence that DDT is associated with NHL, our findings for p,p'-DDE do not support an association.

10.
Org Lett ; 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31820650

RESUMO

The first catalytic asymmetric alkenylation of isatin imines is described. The reaction, which is promoted by a chiral bis(oxazoline)-copper complex, gives structurally diverse 3-alkenyl-3-aminooxindole derivatives in excellent yields, and with excellent diastereoselectivities and high-to-excellent enantioselectivities. The products can be readily converted to polycyclic indole derivatives without loss of enantioselectivity. A plausible chirality-induced mechanism is proposed to explain the observed stereoselective control.

11.
Curr Mol Med ; 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31808386

RESUMO

OBJECTIVE: To Investigate The Relationship Between Genetic Polymorphisms Of Rxrg Rs1467664, Rs3753898 And The Genetic Susceptibility Of Type 2 Diabetes In Chinese Han Population From South China. METHODS: In Our Case-Control Study, Single-Nucleotide Polymorphisms (Snps) Rs1467664 And Rs3753898 Were Genotyped By Snpscantm Kit In 1092 Patients With T2d As Cases And 1092 Normal Persons As Controls. The Distributions Of Genotype And Allele Frequencies In Two Groups Was Analyzed By Spss 20.0 Software. RESULTS: The Distribution Of Genotypes And Alleles Of Rxrg Rs3753898 Was Statistically Significant Between Two Groups, But There Was No Significant Difference In The Distribution Of Genotypes And Alleles Of The Rs1467664. Before And After The Adjustment Of Age, Sex And Bmi, Rs3753898 In The Two Groups Had Statistical Significance Under The Additive, Dominant And Recessive Models (P<0.05), But No Statistical Differences Were Found Under The Overdominance And Co-Dominant Genetic Models (P>0.05). There Was No Significant Difference In The Genetic Models Of Rs1467664 Between The Two Groups (P>0.05). The Haplotype Which Consists Of Rs1467664 Allele T And Rs3753898 Allele A Was A High Risk Factor For T2d, Or=1.27, 95% Ci (1.09-1.47), Padj=0.002. CONCLUSION: Our Results Showed That The Single Nucleotide Polymorphism Of Rxrg Rs3753898 May Be Related To The Genetic Susceptibility Of Type 2 Diabetes. The Haplotype Consisting Of The Allele T Of Rs1467664 And The Allele A Of Rs3753898 Is A Risk Factor For Type 2 Diabetes, Suggesting That Genetic Variation Of Rxrg Gene May Be The Genetic Cause Of Diabetes Mellitus In Chinese Han Population.

12.
Clin Cardiol ; 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31785026

RESUMO

BACKGROUND: To investigate the incidence of new-onset atrial fibrillation (AF) among subjects with pneumoconiosis using the Taiwan National Health Insurance Research Database. HYPOTHESIS: Pneumoconiosis patients are at an increased risk of AF. METHODS: A total of 12 209 pneumoconiosis patients were in the exposure cohort. Patients without pneumoconiosis were included as the comparison cohort. Both cohorts were matched by gender, age, comorbidity, and index year in a 1:1 manner. Multivariable cox proportional hazard model was used to calculate the adjusted hazard ratios (HRs) after adjustment for age, sex, and all comorbidities. RESULTS: The risk of AF in pneumoconiosis patients was 1.30-fold higher than that of controls (95% CI = 1.17-1.44) was the key finding. CONCLUSIONS: Pneumoconiosis is associated with increased risk of incident AF.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31813194

RESUMO

OBJECTIVE: To compare the efficacy of subthalamic nucleus (STN) and globus pallidus internus (GPi) deep brain stimulation (DBS) on reducing levodopa-induced dyskinesia (LID) in Parkinson's disease, and to explore the potential underlying mechanisms. METHODS: We retrospectively assessed clinical outcomes in 43 patients with preoperative LID who underwent DBS targeting the STN (20/43) or GPi (23/43). The primary clinical outcome was the change from baseline in the Unified Dyskinesia Rating Scale (UDysRS) and secondary outcomes included changes in the total daily levodopa equivalent dose, the drug-off Unified Parkinson Disease Rating Scale Part Ⅲ at the last follow-up (median, 18 months), adverse effects, and programming settings. Correlation analysis was used to find potential associated factors that could be used to predict the efficacy of DBS for dyskinesia management. RESULTS: Compared to baseline, both the STN group and the GPi group showed significant improvement in LID with 60.73 ± 40.29% (mean ± standard deviation) and 93.78 ± 14.15% improvement, respectively, according to the UDysRS score. Furthermore, GPi-DBS provided greater clinical benefit in the improvement of dyskinesia (P < 0.05) compared to the STN. Compared to the GPi group, the levodopa equivalent dose reduction was greater in the STN group at the last follow-up (43.81% vs. 13.29%, P < 0.05). For the correlation analysis, the improvement in the UDysRS outcomes were significantly associated with a reduction in levodopa equivalent dose in the STN group (r = 0.543, P = 0.013), but not in the GPi group (r = -0.056, P = 0.801). INTERPRETATION: Both STN and GPi-DBS have a beneficial effect on LID but GPi-DBS provided greater anti-dyskinetic effects. Dyskinesia suppression for STN-DBS may depend on the reduction of levodopa equivalent dose. Unlike the STN, GPi-DBS might exert a direct and independent anti-dyskinesia effect.

14.
Onco Targets Ther ; 12: 9767-9781, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31814735

RESUMO

Purpose: Ubiquitin-conjugating enzyme E2S (UBE2S) is important for the development and progression of several types of cancer. However, neither the role of UBE2S in pancreatic cancer nor its mechanism is clear. Methods: We analyzed three GEO datasets to obtain 150 differentially expressed genes (DEGs) between pancreatic ductal adenocarcinoma (PDAC) and non-cancerous samples. Moreover, GSEA and mutation analysis were also done for UBE2S. The UBE2S expression in PDAC was measured by immunohistochemistry and qRT-PCR. Colony formation, scratch wound-healing and tumor growth assays were conducted to examine the effect of UBE2S on PDAC cells. Migration was detected using Transwell assay. UBE2S knockdown pancreatic cells were treated with proteasome inhibitor MG132. Immunofluorescence was undertaken for interaction between UBE2S and VHL. The expression of Snail and Twist1 and the changes of HIF-1α/STAT3 pathway were detected by Western blotting. Results: The mRNA of UBE2S was significantly upregulated in human pancreatic cancer compared to normal tissues. Immunohistochemistry confirmed that the protein level of UBE2S increased in tissue microarrays (TMAs) and was associated with lymph nodes metastasis and distant metastasis. Conclusion: UBE2S could enhance EMT by the VHL/HIF-1α/STAT3 pathway via the ubiquitin-proteasome system. Co-expression of CDC20 may represent a novel and promising therapeutic target for the patients with PDAC.

15.
Ageing Res Rev ; 57: 100997, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31816444

RESUMO

Mitochondria, which are cell compartments that are widely present in eukaryotic cells, have been shown to be involved in a variety of synthetic, metabolic, and signaling processes, thereby playing a vital role in cells. The mitochondrial unfolded protein response (mtUPR) is a response in which mitochondria reverse the signal to the nucleus and maintain mitochondrial protein homeostasis when unfolded and misfolded proteins continue to accumulate. Multiple neurodegeneration diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and familial amyotrophic lateral sclerosis (fALS), are public health challenges. Every year, countless efforts are expended trying to clarify the pathogenesis and treatment of neurological disorders, which are associated with mitochondrial dysfunction to some extent. Numerous studies have shown that mtUPR is involved in and plays an important role in the pathogenesis of neurological disorders, but the exact mechanism of the disorders is still unclear. Further study of the process of mtUPR in neurological disorders can help us more accurately understand their pathogenesis in order to provide new therapeutic targets. In this paper, we briefly review mtUPR signaling in Caenorhabditis elegans (C. elegans) and mammals and summarize the role of mtUPR in neurodegeneration diseases, including AD, PD and fALS.

16.
J Affect Disord ; 263: 60-63, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31818797

RESUMO

BACKGROUND: The high prevalence of depression in general population was related to its social-demographics and cognitive performance. However, no studies investigated the prevalence of depression, its social-demographic and cognitive correlates in psychiatric medical staff. Thus, the aims of this study investigated the prevalence, social-demographic and cognitive correlates of depression in Chinese psychiatric medical staff. METHODS: 186 Chinese psychiatric medical staff were enrolled in Wenzhou Kangning Hospital. Depressive symptom score was assessed by the Self-rating Depression Scale (SDS). Cognition was assessed by the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). RESULTS: The prevalence of depression was 17.74% in these medical staff. The RBANS total score in participants with depressive symptom was significantly lower than that in participants with not depressive symptom after controlling for the confounding variables. The Person correlation analysis found that the normal SDS score in these medical staff was significantly related to age, education, occupations, RBANS total score and subscale scores. Stepwise multivariate regression analysis further identified that age and RBANS total score were significantly associated with the normal SDS score in these medical staff. LIMITATIONS: The limitations included cross-sectional study design, the small sample size, and the self-rating scale of depression. CONCLUSIONS: The prevalence of depression in Chinese psychiatric medical staff was higher in comparison with Chinese general population, but lower in comparison with Chinese medical staff. Cognitive deficits might be considered a core feather of depression that should be a valuable target for future interventions. Age influenced depressive symptom in these medical staff .

17.
Ann Thorac Surg ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794736

RESUMO

For McKeown esophagectomy, gastric tube is widely used for constructing esophagogastrotomy. Traditionally, the gastric fundus is preserved for gastric tube formation in order to provide adequate length of the gastric conduit for neck anastomosis. However, gastric stump necrosis, anastomotic leakage, and postoperative gastric tube dilatation are the common postoperative complications for McKeown esophagectomy using traditional gastric tube. Here, we reported a novel coniform gastric tube shaping technique to maximally avoid conduit dilatation after the McKeown esophagectomy without nasogastric tube placement, while simultaneously maintain a tension-free and well-perfused anastomosis.

18.
Light Sci Appl ; 8: 106, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31798845

RESUMO

A high-performance and broadband heterojunction photodetector has been successfully fabricated. The heterostructure device is based on a uniform and pinhole-free perovskite film constructed on top of a single-crystal germanium layer. The perovskite/germanium photodetector shows enhanced performance and a broad spectrum compared with the single-material-based device. The photon response properties are characterized in detail from the visible to near-infrared spectrum. At an optical fibre communication wavelength of 1550 nm, the heterojunction device exhibits the highest responsivity of 1.4 A/W. The performance is promoted because of an antireflection perovskite coating, the thickness of which is optimized to 150 nm at the telecommunication band. At a visible light wavelength of 680 nm, the device shows outstanding responsivity and detectivity of 228 A/W and 1.6 × 1010 Jones, respectively. These excellent properties arise from the photoconductive gain boost in the heterostructure device. The presented heterojunction photodetector provides a competitive approach for wide-spectrum photodetection from visible to optical communication areas. Based on the distinguished capacity of light detection and harvesting from the visible to near-infrared spectrum, the designed germanium/perovskite heterostructure configuration is believed to provide new building blocks for novel optoelectronic devices.

19.
ACS Nano ; 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31800218

RESUMO

The continuing increase of the efficiency of perovskite solar cells has pushed the internal quantum efficiency approaching 100%, which means the light-to-carrier and then the following carrier transportation and extraction are no longer limiting factors in photoelectric conversion efficiency of perovskite solar cells. However, the optimal efficiency is still far lower than the Shockley-Queisser efficiency limit, especially for those inverted perovskite solar cells, indicating that a significant fraction of light does not transmit into the active perovskite layer to be absorbed there. Here, a planar inverted perovskite solar cell (ITO/PTAA/perovskite/PC61BM/bathocuproine (BCP)/Ag) is chosen as an example, and we show that its external quantum efficiency (EQE) can be significantly improved by simply texturing the poly[bis (4-phenyl)(2,4,6-trimethylphenyl)amine] (PTAA) layer. By washing the film prepared from a mixed polymer solution of PTAA and polystyrene (PS), a textured PTAA/perovskite interface is introduced on the light-input side of perovskite to inhibit internal optical reflection. The reduction of optical loss by this simple texture method increases the EQE and then the photocurrent of the ITO/PTAA/perovskite/PC61BM/BCP/Ag device with the magnitude of about 10%. At the same time, this textured PTAA benefits the band edge absorption in this planar solar cell. The large increase of the short-circuit current together with the increase of fill factor pushes the efficiency of this inverted perovskite solar cell from 18.3% up to an efficiency over 20.8%. By using an antireflection coating on glass to let more light into the device, the efficiency is further improved to 21.6%, further demonstrating the importance of light management in perovskite solar cells.

20.
Front Microbiol ; 10: 2580, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31803151

RESUMO

Nitrite in a water environment is very harmful to humans and aquatic animals. A novel aerobic denitrifying bacterium able to utilize NO 2 - -N as the only nitrogen source was isolated for the purpose of removing nitrite from water, which was identified as Acinetobacter sp. and named as YT03. The growth and denitrification activity of strain YT03 was assessed comprehensively. Results showed that the nitrite in water with an initial concentration of 10 mg L-1 could be completely removed within 6 h by strain YT03, and the optimal conditions for strain YT03 to remove nitrite were as follows: sodium succinate as the carbon source, C/N ratio of 16, pH of 6.5, temperature of 30°C, and shaking speed of 250 rpm. An RNA-Seq transcriptome analysis was used to find genes associated with nitrite removal. Compared with the removal of ammonia nitrogen, 47 genes were significantly differentially expressed, including 20 up-regulated and 27 down-regulated genes, mainly involved in the transport process, biosynthetic process, and so on. And among the differentially expressed genes, C4-dicarboxylate transporter (DctA) and nitrate/nitrite transporter (Nrt) might be of importance for the efficient utilization of carbon and nitrogen sources in aerobic nitrite denitrification with sodium succinate by strain YT03.

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