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1.
J Phys Condens Matter ; 32(8): 085801, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31536972

RESUMO

Layered transition metal trihalides ABX3 are promising candidate materials for monolayer magnets. In this paper, we investigated single-layer CrXSe3 (X = Sn, Ge, Si) as monolayer ferromagnetic semiconductors (FMS). Firstly, our calculated interlayer binding energies and mechanical properties demonstrate the feasibility of obtaining the free-standing monolayer CrXSe3 from the layered van der Waals crystal CrXSe3 via mechanical exfoliating method. Plus, we find that the ferromagnetic (FM) super-exchange interaction dominates over the anti-ferromagnetic (AFM) direct-exchange interactions, making CrSnSe3 and CrGeSe3 monolayers FM with the magnetic moments of 6.0 µ B per unit cell. Particularly, the FM configurations of CrSnSe3 and CrGeSe3 monolayers become more stable under the increasing tensile strain, and CrSiSe3 converts to FM from AFM under biaxial tensile strain larger than 2%. Additionally, the three monolayers CrXSe3 are all semiconducting with energy band gaps of 0.76, 0.87 and 1.1 eV for X being Sn, Ge and Si, respectively. Our results suggest CrXSe3 as monolayer FMS hold promising potential in spintronics.

2.
Nat Commun ; 10(1): 5525, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31797925

RESUMO

CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequencing of Cas9-treated rhesus monkeys. We only find a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected off-target mutations (OTMs) were detected. Furthermore, the long-read sequencing data does not detect large structural variants in the target region.

3.
Addict Behav ; 102: 106203, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31801104

RESUMO

BACKGROUND: Studies have proven that forced break can elicit strong psychological cravings for addictive behaviors. This phenomenon could create an excellent situation to study the neural underpinnings of addiction. The current study explores brain features during a cue-reactivity task in Internet gaming disorder (IGD) when participants were forced to stop their gaming behaviors. METHODS: Forty-nine IGD subjects and forty-nine matched recreational Internet game users (RGU) were asked to complete a cue-reactivity task when their ongoing gaming behaviors were forced to break. We compared their brain responses to gaming cues and tried to find specific features associated with IGD. RESULTS: Compared with RGU, the IGD subjects showed decreased activation in the anterior cingulate cortex (ACC), parahippocampal gyrus, and dorsolateral prefrontal cortex (DLPFC). Significant negative correlations were observed between self-reported gaming cravings and the baseline activation level (bate value) of the ACC, DLPFC, and parahippocampal gyrus. CONCLUSIONS: IGD subjects were unable to suppress their gaming cravings after unexpectedly forced break. This result could also explain why RGU subjects are able to play online games without developing dependence.

4.
Int J Immunogenet ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31746120

RESUMO

Immunological inflammatory reaction is one of the key links in the occurrence and development of post-traumatic osteomyelitis after microbial invasion. Growing evidence suggests complex interactions between IFN-γ and bone remodelling cells. However, potential association of IFN-γ gene polymorphism with susceptibility to post-traumatic osteomyelitis remains unclear. This study aimed to investigate the potential link between IFN-γ +874T/A polymorphism and risk of developing post-traumatic osteomyelitis. A total of 189 patients with post-traumatic osteomyelitis and 200 healthy controls were enrolled for genotyping using the SNaPshot genotyping method. Statistically significant associations were found between the gene polymorphism and the risk of post-traumatic osteomyelitis by dominant model (AA + AT vs. TT, OR = 1.820, p = .017) and heterozygous model (AT vs. TT, OR = 1.781, p = .029). Moreover, the frequency of mutant allele A was significantly higher in the patients than that in the healthy controls (15.07% vs. 9.25%, OR = 1.742, p = .013). IFN-γ +874T/A polymorphism may contribute to the increased susceptibility to post-traumatic osteomyelitis.

5.
Zootaxa ; 4590(3): zootaxa.4590.3.5, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31716089

RESUMO

Pangrapta Hübner, 1818 was erected for the North American species P. decoralis Hübner, 1818 as its type species. The genus Pangrapta presently consists of about 100 species, and is widely distributed in China and surrounding regions, as well as in the New World (Hu Wang, 2013). In this article, three new species, P. nanlingensis sp. n., P. jianfenglingensis sp. n. and P. hainanensis sp. n., are described from China. Each of them is superficially similar to the species, P. neorecusata (Hu Wang, 2013), P. perturbans (Walker, 1858) and P. pannosoides (Holloway, 2005), correspondently. The known Southeast Asian species, P. shivula (Guenée, 1852), is reported for the first time from China. Adults and genitalia are illustrated. The type series of all described species (including holotypes) are deposited in Southwest University of Science and Technology (SWUST), Mianyang, China.


Assuntos
Lepidópteros , Mariposas , Animais , China , Genitália
6.
Zootaxa ; 4586(2): zootaxa.4586.2.5, 2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-31716131

RESUMO

The paper describes three new species of Meganola (M. subseima sp. n., M. rongjiangensis sp. n. and M. wulongensis sp. n.) from China. Meganola mediofascia (Inoue, 1958) is reported for the first time from China. Adults and genitalia are illustrated.


Assuntos
Lepidópteros , Mariposas , Distribuição Animal , Animais , China , Genitália
7.
Artigo em Inglês | MEDLINE | ID: mdl-31725070

RESUMO

OBJECTIVES: The aim of this project was to improve early postoperative mobilization in patients undergoing abdominal surgery according to best practice. INTRODUCTION: Early mobilization is a crucial element of postoperative care; however, there are challenges implementing early mobilization protocols in daily practice. This project used the evidence to improve awareness and practice of early mobilization in patients undergoing abdominal surgery. METHODS: This study utilized clinical audit strategies under the JBI Practical Application of Clinical Evidence System (JBI PACES) module. An audit-feedback cycle was used from April 2018 to August 2018. The baseline audit was conducted using 18 nurses and 30 patients in a general surgery ward. The Getting Research into Practice audit and feedback tool was used to identify barriers, strategies, resources and outcomes. After implementing evidence-based strategies, a re-audit was conducted following the same number of samples and audit criteria. We analyzed the compliance with best practice and its impact on length of hospitalization, postoperative physical activities, gastrointestinal function and complications. RESULTS: After implementing best-practice strategies, the compliance rate of the six criteria improved as follows: criterion 1 from 0% to 100% (P = 0.000), criterion 2 from 87% to 100% (χ = 4.29, P = 0.038), criterion 3 from 60% to 70% (χ = 6.67, P = 0.010), criterion 4 from 7% to 79% (χ = 52.55, P = 0.000), criterion 5 from 40% to 70% (χ = 35.00, P = 0.000), and criterion 6 from 0% to 100% (P = 0.000). The differences in the length of hospitalization and physical activities between the pre-implementation and post-implementation were statistically significant (all P < 0.05). The rate of postoperative complications did not show a significant difference because of low occurrence. CONCLUSIONS: The results indicate that evidence-based practice is an effective method for enhancing early recovery in patients undergoing abdominal surgery through promoting early mobilization. Sustaining best practice should continue through further follow-up audits.

8.
J Cell Mol Med ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31725199

RESUMO

Bone homeostasis is delicately orchestrated by osteoblasts and osteoclasts. Various pathological bone loss situations result from the overactivated osteoclastogenesis. Receptor activator of nuclear factor κB ligand (RANKL)-activated NF-κB and MAPK pathways is vital for osteoclastogenesis. Here, we for the first time explored the effects of l-tetrahydropalmatine (l-THP), an active alkaloid derived from corydalis, on the formation and function of osteoclasts in vitro and in vivo. In RAW264.7 cells and bone marrow monocytes cells (BMMCs), l-THP inhibited osteoclastic differentiation at the early stage, down-regulated transcription level of osteoclastogenesis-related genes and impaired osteoclasts functions. Mechanically, Western blot showed that l-THP inhibited the phosphorylation of P50, P65, IκB, ERK, JNK and P38, and the electrophoretic mobility shift assay (EMSA) revealed that DNA binding activity of NF-κB was suppressed, ultimately inhibiting the expression of nuclear factor of activated T cells (NFATc1). Besides, Co-immunoprecipitation indicated that l-THP blocked the interactions of RANK and TNF receptor associated factor 6 (TRAF6) at an upstream site. In vivo, l-THP significantly inhibited ovariectomy-induced bone loss and osteoclastogenesis in mice. Collectively, our study demonstrated that l-THP suppressed osteoclastogenesis by blocking RANK-TRAF6 interactions and inhibiting NF-κB and MAPK pathways. l-THP is a promising agent for treating osteoclastogenesis-related diseases such as post-menopausal osteoporosis.

9.
J Surg Res ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31679801

RESUMO

BACKGROUND: Currently, very limited information is available regarding the economic burdens of patients with extremity post-traumatic osteomyelitis (OM). This study aimed to investigate direct health care costs and utilization for inpatients with extremity post-traumatic OM and analyze its constituent ratios and influencing factors in Southern China. METHODS: We searched in the electronic medical record system for inpatients who had received surgical interventions at our department between 2013 and 2016 for extremity post-traumatic OM. Data of direct health care costs incurred during their hospitalizations were collected in six main categories (service, diagnosis, treatment, materials, pharmaceuticals, and miscellaneous expenses). In addition, data of total medical costs for contemporaneous inpatients with non-post-traumatic OM were also collected as controls. RESULTS: A total of 278 post-traumatic OM and 10,420 controls were included. The median cost for the post-traumatic OM inpatients was $10,504 US dollars, 4.8-fold higher than that for those with non-post-traumatic OM ($2189, P < 0.001). The direct cost in the category of materials accounted for the largest proportion (61%), followed by that in pharmaceuticals (12%) and treatment (11%). The median number of hospital admissions for post-traumatic OM patients was 1 time, with a median length-of-stay of 22 d. The most influencing factors for the health care costs of the post-traumatic OM inpatients were use of an external fixator ($16,016 for those who used versus $4956 for those who did not, P < 0.001), external fixator type ($19,563 for ring fixator versus $14,966 for rail fixator, P < 0.001), infection site ($13,755 for tibia, $14,216 for femur and $5673 for calcaneus, P < 0.001), and infection-associated injury type ($12,890 for infection after open fracture versus $8087 for infection after closed fracture, P = 0.001). CONCLUSIONS: An unexpectedly large proportion of the direct health care costs for inpatients with extremity post-traumatic OM went to cover an external fixator, with expenses for pharmaceuticals and treatment accounting for only a little more than the tenth of the total health care costs. Use of external fixator, external fixator type, infection site, and infection-associated injury type directly influenced the health care costs.

10.
Physiol Genomics ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31682178

RESUMO

Long non-coding RNAs have been implicated in the development and progression of atherosclerosis. However, the expression and mechanism of action of lncRNAs in atherosclerosis are still unclear. We implemented microarray analysis in human advanced atherosclerotic plaques and normal arterial intimae to detect the lncRNA and mRNA expression profile. Gene Ontology functional enrichment and pathway analyses were applied to explore the potential functions and pathways involved in the pathogenesis of atherosclerosis. A total of 236 lncRNAs and 488 mRNAs were selected for further Ingenuity Pathway Analysis. Moreover, qRT-PCR tests of most selected lncRNAs and mRNAs with high fold changes were consistent with the microarray data. We also performed ELISA to investigate the corresponding proteins levels of selected genes and showed that serum levels of SPP1, CD36, ATP6V0D2, CHI3L1, MYH11 and BDNF were differentially expressed in patients with coronary heart disease compared with healthy subjects.These proteins correlated with some biochemical parameters used in the diagnosis of cardiovascular diseases. Furthermore, receiver operating characteristic analysis showed a favorable diagnostic performance.The microarray profiling analysis and validation of differentially-expressed lncRNAs and mRNAs in atherosclerosis not only provide new insights into the pathogenesis of this disease, but may also reveal new biomarkers for its diagnosis and treatment.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31769960

RESUMO

There have been more challenging for hydrogel actuators to meet the combined requirement of discoloration, complex deformation and simple preparation. Structural coloration is widely used to fabricate discolor hydrogel via micro-rearrangement of photonic crystals in the hydrogel framework. However, precise regulation is usually required. Besides, the macro-optical properties are unstable. Herein, we develop a hierarchical and gradient hydrogel actuator with complex deformation and color-changing functions using electrophoresis method. A simple but effective strategy is presented for fabrication of hierarchical hydrogel composed of homopolymers and copolymers via salt-induced microphase separation during the polymerization of the N-isopropylacrylamide (NIPAm) and [2-(Methacryloyloxy)ethyl]trimethylammonium chloride (DMC). Meanwhile a gradient distribution of DMC is also formed during the polymerization due to migration of DMC under electric field. The hierarchical and gradient structure are characterized by CLSM, SAXS, temperature-variable FTIR and etc. The discolor mechanism is proposed. The as-prepared hydrogel can undergo fast and complex thermo-triggered deformation and discoloration. Bionic movements of discolor flowering and information encryption are successfully demonstrated, promising great potential in the application of biomimetic materials.

12.
Opt Lett ; 44(23): 5751-5754, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31774770

RESUMO

The digitization of the complete shape of real objects has essential applications in fields of intelligent manufacturing, industrial detection, and reverse modeling. In order to build the full geometric models of rigid objects, the object must be moved relative to the measurement system (or the scanner must be moved relative to the object) to obtain and integrate views of the object from all sides, which not only complicates the system configuration but makes the whole process time-consuming. In this Letter, we present a high-resolution real-time 360° three-dimensional (3D) model reconstruction method that allows one to rotate an object manually and see a continuously updated 3D model during the scanning process. A multi-view fringe projection profilometry system acquires high-precision depth information about a handheld object from different perspectives and, meanwhile, the multiple views are aligned and merged together in real time. Our system employs stereo phase unwrapping and an adaptive depth constraint that can unwrap the phase of dense fringe images robustly without increasing the number of captured patterns. We then develop an efficient coarse-to-fine registration strategy to match the 3D surface segments rapidly. Our experiments demonstrate that our method can reconstruct the high-precision complete 3D model of complex objects under arbitrary rotation without any instrument assist and expensive pre/post-processing.

13.
J Bioenerg Biomembr ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31768721

RESUMO

Myocardial ischaemia reperfusion injury (MIRI) is considered the primary cause of death in patients with cardiovascular diseases. Recently, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been found to be involved in the pathogenesis of MIRI. However, whether lncRNA ROR and miR-124-3p play roles in MIRI and the underlying mechanism remain undetermined. HCMs were exposed to hypoxic conditions for 2 h followed by re-oxygenation (H/R) treatment. Expression of miR-124-3p and lncRNA ROR in HCMs was measured by qRT-PCR. TRAF6 expression was evaluated by qRT-PCR and western blotting. ELISA and qRT-PCR were conducted to assess the production of TNF-α, IL-6, and IL-1ß. The interaction between miR-124-3p and TRAF6, as well as between miR-124-3p and lncRNA ROR, was verified by dual-luciferase reporter assay. Cell apoptosis was detected by flow cytometry analysis. Our data revealed that miR-124-3p was significantly downregulated, while TRAF6 and lncRNA ROR were upregulated in both MIRI rat model and H/R treated HCMs. Overexpression of miR-124-3p reversed the H/R-induced cell apoptosis and upregulation of TNF-α, IL-6, and IL-1ß. Mechanistically, miR-124-3p bound and negatively regulated TRAF6 expression in HCMs. Moreover, TRAF6 overexpression significantly blocked the effects of miR-124-3p mimics on cell apoptosis and inflammatory response of HCMs, which involved the NF-κB pathway. Further analysis showed that lncRNA ROR sponged and negatively regulated miR-124-3p in HCMs. Overexpression of IL-1ß was demonstrated to promote H/R induced cell apoptosis in HCMs. In addition, overexpression of ROR further enhanced the H/R-induced inflammation and cell apoptosis through its action on miR-124-3p. The lncRNA ROR/miR-124-3p/TRAF6 axis regulated the H/R-induced cell apoptosis and inflammatory response of HCMs.

17.
Biomed Pharmacother ; 120: 109535, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31610428

RESUMO

With increasing demand, the development of new natural antioxidants has become a primary direction of scientific research. We previously identified a short gene-encoded peptide (OA-VI12) from Odorrana andersonii frog skin secretions that exerted direct scavenging capacity against free radicals, suggesting a possible function in protecting skin against photodamage caused by their high-altitude habitat. In the current research, we examined the effects of OA-VI12 on both UVB-irradiation and hydrogen peroxide-induced oxidative stress models established with human immortalized keratinocytes. In addition, we identified the differentially expressed genes (DEGs) in the oxidative stress and OA-VI12 groups and further performed transcriptome as well as functional and pathway enrichment analyses. Results showed that OA-VI12 protected cell viability, promoted the release of catalase, and decreased the levels of lactate dehydrogenase and reactive oxygen species. Moreover, the peptide promoted the production of superoxide dismutase and glutathione, alleviated epidermis and dermis thickness, and decreased the production of light spots and collagen fibers in skin from the photo-injured mouse model. Kyoto Encyclopedia of Genes and Genomes analysis showed mitogen-activated protein kinase (MAPK) to be the most abundant signaling pathway. Gene Ontology (GO) analysis indicated that the top 55 significantly enriched GO terms mainly involved cellular processes, parts, and binding. These results revealed the beneficial role of the small molecule gene-encoding antioxidant peptide (OA-VI12) and its potential application as a protective agent against photodamage.

18.
Cancer Immunol Res ; 7(12): 2065-2073, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31615816

RESUMO

Current gastric cancer staging alone cannot predict prognosis and adjuvant chemotherapy benefits in stage II and III gastric cancer. Tumor immune microenvironment biomarkers and tumor-cell chemosensitivity might add predictive value to staging. This study aimed to construct a predictive signature integrating tumor immune microenvironment and chemosensitivity-related features to improve the prediction of survival and adjuvant chemotherapy benefits in patients with stage II to III gastric cancer. We used IHC to assess 26 features related to tumor, stroma, and chemosensitivity in tumors from 223 patients and evaluated the association of the features with disease-free survival (DFS) and overall survival (OS). Support vector machine (SVM)-based methods were used to develop the predictive signature, which we call the SVM signature. Validation of the signature was performed in two independent cohorts of 445 patients. The diagnostic signature integrated seven features: CD3+ cells at the invasive margin (CD3 IM), CD8+ cells at the IM (CD8 IM), CD45RO+ cells in the center of tumors (CD45RO CT), CD66b+ cells at the IM (CD66b IM), CD34+ cells, periostin, and cyclooxygenase-2. Patients fell into low- and high-SVM groups with significant differences in 5-year DFS and OS in the training and validation cohorts (all P < 0.001). The signature was an independent prognosis indicator in multivariate analysis in each cohort. The signature had better prognostic value than various clinicopathologic risk factors and single features. High-SVM patients exhibited a favorable response to adjuvant chemotherapy. Thus, this SVM signature predicted survival and has the potential for identifying patients with stage II and III gastric cancer who could benefit from adjuvant chemotherapy.

19.
Biomater Sci ; 7(12): 5492-5505, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31663543

RESUMO

Titanium-based materials have been long regarded as effective bone implants for clinical use, yet the corresponding osteointegration ability needs to be optimized. This challenge can be overcome by fabricating titanium (Ti) materials with physiological functions. In this study, peptide LL-37-loaded silk fibroin nanoparticles (SFNPs) were immobilized on a titanium surface to facilitate osteointegration by regulating the physiological functions of mesenchymal stem cells (MSCs) and macrophages. According to our results, the cell viability, recruitment and paracrine responses of MSCs and macrophages were improved by the modified Ti samples. MSC differentiation was promoted by the macrophages incubated on the modified Ti samples, and the phenotype switch of macrophages was also modulated by the MSCs incubated on the modified Ti samples. In vivo studies proved that the modified Ti implant induced MSC and macrophage recruitments to injury sites and the inflammatory response was positively regulated. Moreover, better bone formation was achieved around the modified Ti implant 28 days after surgery. This suggested that the immobilization of peptide LL-37-loaded SFNPs on a titanium surface improves osteointegration via the regulation of physiological functions of MSCs and macrophages.

20.
J Am Chem Soc ; 141(45): 17989-17994, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31661269

RESUMO

The nitroxyl radical 1-methyl-2-azaadamantane N-oxyl (Me-AZADO) exhibits magnetic bistability arising from a radical/dimer interconversion. The transition from the rotationally disordered paramagnetic plastic crystal, Me-AZADO, to the ordered diamagnetic crystalline phase, (Me-AZADO)2, has been conclusively demonstrated by crystal structure determination from high-resolution powder diffraction data and by solid-state NMR spectroscopy. The phase change is characterized by a wide thermal hysteresis with high sensitivity to even small applied pressures. The molecular dynamics of the phase transition from the plastic crystal to the conventional crystalline phase has been tracked by solid-state (1H and 13C) NMR and EPR spectroscopies.

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