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1.
Sci Data ; 11(1): 169, 2024 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-38316816

RESUMO

Compared to commercial chickens, local breeds exhibit better in meat quality and flavour, but the productivity (e.g., growth rate, body weight) of local chicken breeds is rather low. Genetic analysis based on whole-genome sequencing contributes to elucidating the genetic markers or putative candidate genes related to some economic traits, facilitating the improvement of production performance, the acceleration of breeding progress, and the conservation of genetic resources. Here, a total of 209 local chickens from 13 breeds were investigated, and the observation of approximately 91.4% high-quality sequences (Q30 > 90%) and a mapping rate over 99% for each individual indicated good results of this study, as confirmed by a genome coverage of 97.6%. Over 19 million single nucleotide polymorphisms (SNPs) and 1.98 million insertion-deletions (InDels) were identified using the reference genome (GRCg7b), further contributing to the public database. This dataset provides valuable resources for studying genetic diversity and adaptation and for the cultivation of new chicken breeds/lines.


Assuntos
Galinhas , Genoma , Animais , Galinhas/genética , China , Marcadores Genéticos , Variação Genética , Fenótipo , Polimorfismo de Nucleotídeo Único , Sequenciamento Completo do Genoma
2.
Int J Mol Sci ; 25(2)2024 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-38256234

RESUMO

Circadian rhythm disorders pose major risks to human health and animal production activity, and the hypothalamus is the center of circadian rhythm regulation. However, the epigenetic regulation of circadian rhythm based on farm animal models has been poorly investigated. We collected chicken hypothalamus samples at seven time points in one light/dark cycle and performed long noncoding RNA (lncRNA), circular RNA (circRNA), and mRNA sequencing to detect biomarkers associated with circadian rhythm. We enhanced the comprehensive expression profiling of ncRNAs and mRNAs in the hypothalamus and found two gene sets (circadian rhythm and retinal metabolism) associated with the light/dark cycle. Noncoding RNA networks with circadian expression patterns were identified by differential expression and circadian analysis was provided that included 38 lncRNAs, 15 circRNAs, and 200 candidate genes. Three lncRNAs (ENSGALT00000098661, ENSGALT00000100816, and MSTRG.16980.1) and one circRNA (novel_circ_010168) in the ncRNA-mRNA regulatory network were identified as key molecules influencing circadian rhythm by regulating AOX1 in retinal metabolism. These ncRNAs were predicted to be related to pernicious anemia, gonadal, eye disease and other disorders in humans. Together, the findings of this study provide insights into the epigenetic mechanisms of circadian rhythm and reveal AOX1 as a promising target of circadian rhythm regulation.


Assuntos
RNA Longo não Codificante , Animais , Humanos , RNA Longo não Codificante/genética , Epigênese Genética , RNA Circular/genética , Ritmo Circadiano/genética , RNA Mensageiro/genética
3.
ESC Heart Fail ; 11(1): 147-154, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37849442

RESUMO

AIMS: Acute type A aortic dissection (ATAAD) is a life-threatening condition requiring prompt diagnosis and treatment. Surgery is an effective treatment for ATAAD, but the in-hospital mortality rate in the 30 day perioperative period is still as high as 9-30%. It is critical to identify biological factors for preoperative assessment of post-operative survival in patients with ATAAD. METHODS AND RESULTS: This is a retrospective study, investigating the association of combined measurements of d-dimer, C-reactive protein (CRP), and matrix metalloproteinase 9 (MMP9) for 1 year of survival in patients with ATAAD. Data from 247 patients who underwent surgery were analysed, including 89 patients who did not survive and 158 patients who survived within 1 year after surgery. Pearson's correlation analysis was carried out to determine the correlations between CRP in whole blood, d-dimer in plasma, and CRP in whole blood. Receiver operating characteristic (ROC) analysis was used to analyse the value of preoperative whole blood CRP, plasma d-dimer, and serum MMP9 concentration and the combined detection model in predicting death of patients with ATAAD. Deceased patients with ATAAD exhibited higher age, hypertension prevalence, systolic blood pressure, white blood cell count, whole blood CRP, plasma d-dimer, and serum MMP9 levels compared with survivors. Preoperative CRP, d-dimer, and MMP9 levels were significantly higher in patients with ATAAD compared with healthy controls. Positive correlations were observed between CRP and d-dimer, CRP and MMP9, and d-dimer and MMP9 in patients with ATAAD. ROC analysis showed that the combined detection model of CRP, d-dimer, and MMP9 had the highest predictive value for 1 year of survival (area under the curve = 0.88). CONCLUSIONS: Combined measurement of CRP, d-dimer, and MMP9 is associated with 1 year of survival in patients with ATAAD.


Assuntos
Dissecção Aórtica , Proteína C-Reativa , Produtos de Degradação da Fibrina e do Fibrinogênio , Humanos , Proteína C-Reativa/análise , Metaloproteinase 9 da Matriz , Estudos Retrospectivos , Biomarcadores , Dissecção Aórtica/diagnóstico , Dissecção Aórtica/cirurgia
4.
Appl Opt ; 62(29): 7844-7851, 2023 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-37855495

RESUMO

In the development of the Cold Atom Physics Research Rack (CAPR) on board the Chinese Space Station, the laser system plays a critical role in preparing the all-optical 87 R b Bose-Einstein condensates (BECs). An all-fiber laser system has been developed for CAPR to provide the required optical fields for atom interaction and to maintain the beam pointing in long-term operation. The laser system integrates a 780 nm fiber laser system and an all-fiber optical control module for sub-Doppler cooling, as well as an all-fiber 1064 nm laser system for evaporative cooling. The high-power, single-frequency 780 nm lasers are achieved through rare-Earth doped fiber amplification, fiber frequency-doubling, and frequency stabilization technology. The all-fiber optical control module divides the output of the 780 nm laser system into 15 channels and regulates them for cooling, trapping, and probing atoms. Moreover, the power consistency of each pair of cooling beams is ensured by three power tracking modules, which is a prerequisite for maintaining stable MOT and molasses. A high-power, compact, controlled-flexible, and highly stable l064 nm all-fiber laser system employing two-stage ytterbium-doped fiber amplifier (YDFA) technology has been designed for evaporative cooling in the optical dipole trap (ODT). Finally, an all-optical 87 R b BEC is realized with this all-fiber laser system, which provides an alternative solution for trapping and manipulating ultra-cold atoms in challenging environmental conditions.

5.
Int J Mol Sci ; 24(16)2023 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-37628846

RESUMO

Trans-10-hydroxy-2-decenoic acid (10-HDA) is a unique fatty acid found in royal jelly that possesses potential health benefits such as anti-inflammatory. However, further research is needed to fully understand its mechanisms of action and therapeutic potential for inflammation-associated diseases. In this present study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA-seq analyses were conducted to comprehensively analyze the in vitro anti-inflammatory effects of 10-HDA on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Our results demonstrated that 128 differentially expressed metabolites and 1721 differentially expressed genes were identified in the 10-HDA-treated groups compared to the LPS groups. Metabolites were significantly enriched in amino acid metabolism pathways, including methionine metabolism, glycine and serine metabolism, and tryptophan metabolism. The differentially expressed genes enrichment analysis indicated that antigen processing and presentation, NOD-like receptor signaling pathway, and arginine biosynthesis were enriched with the administration of 10-had. The correlation analysis revealed that glycerophospholipid metabolism and s-adenosylmethionine-dependent methylation processes might be involved in the response to the 10-HDA treatment. Overall, the findings from this study showed that 10-HDA might involve the modulation of certain signaling pathways involved in the inflammatory response, but further research is needed to determine the safety and efficacy as a therapeutic agent.


Assuntos
Lipopolissacarídeos , Transcriptoma , Animais , Camundongos , Lipopolissacarídeos/farmacologia , Cromatografia Líquida , Células RAW 264.7 , Espectrometria de Massas em Tandem
6.
Materials (Basel) ; 16(7)2023 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-37049090

RESUMO

In this study, in order to provide proper parameters for the preparation of semisolid billets, the semisolid annealing of hot-rolled 2A14 Al alloy was investigated. The microstructure was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) with an X-ray energy dispersive spectrometer (EDS) and electron backscattered diffraction (EBSD), and scanning transmission electron microscopy (STEM). The XRD results showed that, with an increase in temperature, the θ-Al2Cu equilibrium gradually dissolved in the matrix. The EDS results of SEM and STEM showed a coarse θ-Al2Cu phase, ultrafine precipitate Al(MnFeSi) or (Mn, Fe)Al6 phase, and atomic clusters in the microstructure. The EBSD results showed that the recrystallization mechanism was dominated by continuous static recrystallization (CSRX), homogeneous nucleation occurred when the sample was heated to near solidus temperature, and CSRX occurred at a semisolid temperature. In the process of recrystallization, the microtexture changed from the preferred orientation to a random orientation. Various experimental results showed that static recrystallization (SRX) occurred at a semisolid temperature due to the blocking effect of atomic clusters on the dislocation slip, and the Zener drag effect of fine precipitates on low-angle grain boundaries (LAGBs) disappeared with melting at a semisolid temperature.

7.
Int J Mol Sci ; 24(6)2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36982426

RESUMO

There is a growing risk of pollinators being exposed to multiple fungicides due to the widespread use of fungicides for plant protection. A safety assessment of honeybees exposed to multiple commonly used fungicides is urgently required. Therefore, the acute oral toxicity of the ternary mixed fungicide of ABP (azoxystrobin: boscalid: pyraclostrobin = 1:1:1, m/m/m) was tested on honeybees (Apis cerana cerana), and its sublethal effect on foragers' guts was evaluated. The results showed that the acute oral median lethal concentration (LD50) of ABP for foragers was 12.6 µg a.i./bee. ABP caused disorder of the morphological structure of midgut tissue and affected the intestinal metabolism; the composition and structure of the intestinal microbial community was perturbed, which altered its function. Moreover, the transcripts of genes involved in detoxification and immunity were strongly upregulated with ABP treatment. The study implies that exposure to a fungicide mixture of ABP can cause a series of negative effects on the health of foragers. This work provides a comprehensive understanding of the comprehensive effects of common fungicides on non-target pollinators in the context of ecological risk assessment and the future use of fungicides in agriculture.


Assuntos
Fungicidas Industriais , Microbioma Gastrointestinal , Abelhas , Animais , Fungicidas Industriais/toxicidade
8.
Molecules ; 28(5)2023 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-36903389

RESUMO

In terms of new-generation energy-storing devices, aqueous zinc-ion batteries (AZIBs) are becoming the prime candidates because of their inexpensive nature, inherent safety, environmental benignity and abundant resources. Nevertheless, due to a restrained selection of cathodes, AZIBs often perform unsatisfactorily under long-life cycling and high-rate conditions. Consequently, we propose a facile evaporation-induced self-assembly technique for preparing V2O3@carbonized dictyophora (V2O3@CD) composites, utilizing economical and easily available biomass dictyophora as carbon sources and NH4VO3 as metal sources. When assembled in AZIBs, the V2O3@CD exhibits a high initial discharge capacity of 281.9 mAh g-1 at 50 mA g-1. The discharge capacity is still up to 151.9 mAh g-1 after 1000 cycles at 1 A g-1, showing excellent long-cycle durability. The extraordinary high electrochemical effectiveness of V2O3@CD could be mainly attributed to the formation of porous carbonized dictyophora frame. The formed porous carbon skeleton can ensure efficient electron transport and prevent V2O3 from losing electrical contact due to volume changes caused by Zn2+ intercalation/deintercalation. The strategy of metal-oxide-filled carbonized biomass material may provide insights into developing high-performance AZIBs and other potential energy storage devices, with a wide application range.

9.
Sci Total Environ ; 868: 161637, 2023 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-36649770

RESUMO

The widespread use of fungicides for plant protection has increased the potential for pollinator exposure. This study therefore aimed at assessing the acute and chronic effects of fungicides on pollinators. For this purpose, the acute oral toxicity of the common fungicides azoxystrobin, pyraclostrobin, and boscalid to Eastern honeybee Apis cerana cerena was first evaluated, and the chronic effects on multiple aspects were investigated after exposure to a one-tenth medium lethal dose (LD50) for 10 days. This study revealed that the LD50 values of azoxystrobin, pyraclostrobin and boscalid for adult Eastern honeybees were 12.7 µg/bee, 36.6 µg/bee, and >119 µg/bee, respectively. Midgut epithelial cells revealed that fungicide exposure caused increased intercellular spaces and varying degrees of vacuolization. Exposure to these three fungicides and their binary mixtures significantly affected glycerophospholipid, alanine, aspartate, and glutamate metabolism in Eastern honeybee midguts. Additionally, the relative composition of Lactobacillus, the dominant functional genus in Eastern honeybee guts decreased and microbial balance was disrupted. All fungicides and their mixtures induced strong transcriptional upregulation of genes associated with the immune response and encoding enzymes related to oxidative phosphorylation and metabolism, including abaecin, apidaecin, hymenotaecin, cyp4c3, cyp6a2 and hbg3. Our study provides important insight for understanding the effects of commonly used fungicides on nontarget pollinator and contributes to a more comprehensive assessment of fungicide effects on ecological and environmental safety.


Assuntos
Fungicidas Industriais , Himenópteros , Abelhas , Animais , Fungicidas Industriais/toxicidade
10.
Materials (Basel) ; 17(1)2023 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-38203960

RESUMO

In this work, squeeze casting experiments of flywheel housing components with a large wall thickness difference and a complex shape were carried out with AlSi9Mg aluminum alloy. The defects, microstructures, and mechanical properties under different process parameters were investigated. Furthermore, the local pressurization process was applied to the thick-walled positions to force-feed the cast defects. The mechanical properties and microstructures at these positions were analyzed. The results showed that the surface quality of formed components was good and that local pressurization could effectively reduce the shrinkage cavity and shrinkage porosity in thick walls, but the scope and effect of forced feeding were limited. The optimum process parameters were a pouring temperature of 650 °C, a specific pressure of 48 MPa, a mold temperature of 220 °C, a local pressurization of 800 MPa, and pressure delay times of 15 s (side A) and 17 s (side B). The ultimate tensile strength, yield strength, and elongation of the formed component under validation experiments of the optimum process parameters were 201 MPa, 103 MPa, and 5.1%. Meanwhile, the fine grains of primary α-Al were mainly rosette and equiaxed grains, and the average grain size was about 40 µm. The microstructure of the eutectic silicon was acicular and was prone to segregation under pressure. According to profile morphology, the positions after pressurization were divided into a deformation zone, a direct action zone, and an indirect action zone. The coexistence of as-cast and plastic deformation microstructures was observed. The effect of local pressurization mainly involved a change in the solidification process, plastic deformation, and forced feeding.

11.
Mol Pain ; 18: 17448069221145096, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-36464669

RESUMO

Pain involves both sensory and affective dimensions. The amygdala is a key player in linking nociceptive stimuli to negative emotional behaviors or affective states. Relief of pain is rewarding and activates brain reward circuits. Whether the reward circuit from the ventral tegmental area (VTA) to the central amygdala (CeA) is involved in pain relief remains unexplored. Using a model of experimental postsurgical pain, we found that pain relief elicited conditioned place preference (CPP), activated CeA-projecting dopaminergic cells in the VTA, and decreased dopaminergic D2 receptor expression in the CeA. Activation of the VTA-CeA neural pathway using optogenetic approaches relieved incisional pain. Administration of a D2 receptor agonist reversed the pain relief elicited by light-induced activation of the VTA-CeA pathway. These findings indicate that the VTA-CeA circuit is involved in pain relief in mice via dopamine receptor D2 in the CeA.


Assuntos
Núcleo Central da Amígdala , Área Tegmentar Ventral , Animais , Camundongos , Dor , Receptores Dopaminérgicos
12.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 38(4): 335-340, 2022 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-36414558

RESUMO

Objective: To investigate the effects of miRNA-130a-3p on autophagy and apoptosis induced by LPS in myocardial cells and its molecular mechanisms. Methods: H9C2 cells were divided into five groups: normal control group, LPS model group, miRNA negative control group miRNA-130a-3p mimics group(overexpression of miRNA-130a-3p) and miRNA-130a-3p mimics + LY294002 group(overexpression of miRNA-130a-3p + PI3K inhibitor). The LPS model group was induced by LPS at a final concentration of 10 µg/ml for 24 h. In the miRNA negative control group and miRNA-130a-3p mimics group, negative contro miRNA or miRNA-130a-3p mimics were transfected into H9C2 cells by lipo3000. After 24 h of culture, LPS was added into the medium for 24 hours. In the miRNA -130A-3P mimics + LY294002 group, miRNA -130A-3P mimics was transfected into H9C2 cells by using lipo3000, and LY294002 at a final concentration of 10 µmol/L was added to the culture medium for 24 h, followed by LPS at a concentration of 10 µg/ml for 24 h. The expression of miRNA-130a-3p mRNA in cells was detected by RT-qPCR. The CCK-8 assay was used to detect the cell viability. The contents of TNF-α, IL-6 and IL-1ß were detected by ELISA assay. The contents of SOD and LDH in cell culture medium were detected by colorimetry. Western blot was used to detect the protein expressions of p-PI3K, p-AKT, Bax, Bcl-2, cleaved-caspase-3, LC3 and p62. Results: The results showed that the levels of miRNA-130a-3p mRNA, p-PI3K protein and p-AKT protein in LPS model cells were significantly lower than those in normal control group(P<0.01), and the expressions of p-PI3K, p-AKT protein in miRNA-130a-3p mimics group were increased significantly compared with LPS group(P<0.01,P<0.05). Compared with normal control group, the cell viability was decreased significantly and the contents of TNF-α, IL-6, IL-1ß and LDH were increased significantly(P<0.01), the contents of SOD was decreased significantly in LPS group(P<0.01). The protein expression levels of Bax, cleaved-caspase-3 and p62 were increased significantly, while the expression level of Bcl-2 and LC3II/I ratio were decreased significantly in LPS group(P<0.01). miRNA-130a-3p mimics could increase the cell viability, decrease the contents of TNF-α, IL-6, IL-1ß and LDH(P<0.01,P<0.05), increase the contents of SOD(P<0.05), decrease the expressions of Bax, cleaved caspase-3, p62(P<0.01), promote the expression of Bcl-2(P<0.01) and increase the ratio of LC3II/I(P<0.05). Compared with miRNA-130a-3p mimics group, LY294002 reversed the effects of miRNA-130a-3p mimics on cells. Conclusion: Overexpression miRNA-130a-3p could partly promote autophagy and inhibit cell apoptosis by activating PI3K/AKT signaling pathway to alleviate LPS-induced myocardial injury.


Assuntos
MicroRNAs , Miócitos Cardíacos , Miócitos Cardíacos/metabolismo , Caspase 3 , Lipopolissacarídeos , Proteínas Proto-Oncogênicas c-akt , Fosfatidilinositol 3-Quinases , Fator de Necrose Tumoral alfa , Interleucina-6 , Proteína X Associada a bcl-2 , Apoptose/genética , Autofagia , MicroRNAs/genética , MicroRNAs/metabolismo , Superóxido Dismutase , RNA Mensageiro
13.
Biomater Adv ; 136: 212774, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35929313

RESUMO

Graphene oxide quantum dots (GOQDs) have attracted substantial attention in numerous fields due to their unique physicochemical properties. However, their nanotoxicity and potential for use in biomedicine still require further study. In this work, the effects of GOQD and trans- 10-hydroxy-2-decenoic acid (10-HDA) cotreatment on the immune function of macrophages (RAW264.7 cells) were investigated. In particular, LC/MS-based metabolomics was performed to evaluate the effects of GOQDs on the metabolism of LPS-stimulated macrophages. Herein, we fabricated GOQDs with good dispersibility and a uniform size distribution of approximately 7 nm using a polyimide-pyrolyzed carbon film as the working electrode, a high-voltage graphite electrode as the cathode, and H2O2 as the oxidant. The GOQDs entered the macrophages and emitted green fluorescence under UV irradiation. Cotreatment with GOQDs and 10-HDA induced RAW 264.7 cell proliferation. GOQDs promoted the anti-inflammatory effect of 10-HDA on LPS-stimulated RAW264.7 cells and attenuated the secretion of TNF-α, IL-6, and IL-1ß. The metabolites in RAW264.7 cells treated with GOQDs were significantly different from those in RAW264.7 cells treated with LPS. The enrichment analysis showed that treatment with GOQDs interfered with amino acid metabolism, and lipid metabolism. Our results demonstrate the role of GOQDs in macrophages and provide a basis for their further application in biomedical fields.


Assuntos
Grafite , Pontos Quânticos , Anti-Inflamatórios/farmacologia , Ácidos Graxos Monoinsaturados , Grafite/farmacologia , Peróxido de Hidrogênio/química , Lipopolissacarídeos/farmacologia , Macrófagos , Pontos Quânticos/química
14.
Oxid Med Cell Longev ; 2022: 4472751, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35464771

RESUMO

Background: Recent experimental studies have shown that vegetable oil supplementation ameliorates high-fat diet- (HFD-) induced hyperlipidemia and oxidative stress in mice via modulating hepatic lipid metabolism and the composition of the gut microbiota. The aim of this study was to investigate the efficacy of the Torreya grandis kernel oil (TKO) rich in unpolysaturated fatty acid against hyperlipidemia and gain a deep insight into its potential mechanisms. Methods: Normal mice were randomly divided into three groups: ND (normal diet), LO (normal diet supplement with 4% TKO), and HO (normal diet supplement with 8% TKO). Hyperlipidemia mice were randomly divided into two groups: HFN (normal diet) and HFO (normal diet supplement with 8% TKO). Blood biochemistry and histomorphology were observed; liver RNA-seq, metabolomics, and gut 16S rRNA were analyzed. Results: Continuous supplementation of TKO in normal mice significantly ameliorated serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and free fatty acid (FFA) accumulation, decreased blood glucose and malondialdehyde (MDA), and enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels. According to GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, most differentially expressed genes (DEGs) were significantly enriched in the biosynthesis of unsaturated fatty acid pathways, and significantly changed metabolites (SCMs) might be involved in the metabolism of lipids. High-dose TKO improved gut alpha diversity and beta diversity showing that the microbial community compositions of the five groups were different. Conclusion: Supplementation of TKO functions in the prevention of hyperlipidemia via regulating hepatic lipid metabolism and enhancing microbiota richness in normal mice. Our study is the first to reveal the mechanism of TKO regulating blood lipid levels by using multiomics and promote further studies on TKO for their biological activity.


Assuntos
Microbioma Gastrointestinal , Hiperlipidemias , Taxaceae , Animais , HDL-Colesterol , Dieta Hiperlipídica/efeitos adversos , Hiperlipidemias/metabolismo , Metabolismo dos Lipídeos , Fígado/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , RNA Ribossômico 16S/metabolismo , Taxaceae/metabolismo
15.
Pestic Biochem Physiol ; 180: 104994, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34955187

RESUMO

Honey bees are important and highly efficient pollinators of agricultural crops and have been negatively affected by insecticides in recent years. Circular RNA (circRNA) plays an important role in the regulation of multiple biological and pathological processes; however, its role in the honey bee brain after exposure to dinotefuran is not well understood. Here, the expression profiles and potential modulation networks of circRNAs in the brains of workers (Apis mellifera) were comprehensively investigated using RNA sequencing and bioinformatics. In total, 33, 144, and 211 differentially expressed (DE) circRNAs were identified on the 1st, 5th and 10th days after exposure to dinotefuran, respectively. Enrichment analyses revealed that the host genes of DE circRNAs were enriched in the Hippo signaling pathway-fly, Wnt signaling pathway, and neuroactive ligand-receptor interaction. circ_0002266, circ_0005080, circ_0010239 and circ_0005415 were found to have translational potential due to the presence of an internal ribosome entry site (IRES). An integrated analysis of the DE circRNA-miRNA-mRNA networks suggest that circ_0008898 and circ_0001829 may participate in the immune response to dinotefuran exposure by acting as miRNA sponges. Our results provide invaluable basic data on A. mellifera brain circRNA patterns and a molecular basis for further study of the biological function of circRNAs in the development and immune response of honey bees.


Assuntos
Abelhas , Guanidinas/toxicidade , Neonicotinoides , Nitrocompostos/toxicidade , RNA Circular , Animais , Abelhas/efeitos dos fármacos , Abelhas/genética , Encéfalo/efeitos dos fármacos , Redes Reguladoras de Genes , Via de Sinalização Hippo , Neonicotinoides/toxicidade , Via de Sinalização Wnt
16.
Environ Technol ; 43(9): 1328-1339, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32990178

RESUMO

In this study, three kinds of modified basalt fibre (MBF) were used as biofilm carrier to treat wastewater, mainly for the removal of organic matter and nutrient pollutants, which was evaluated the feasibility of modification by pollutants removal performance. Polydopamine modified basalt fibre (PAD-BF) via the surface coating method were obtained. Polyacrylamide modified basalt fibre (PAM-BF) and maleic anhydride-modified basalt fibre (MAH-BF) via the surface grafting method were prepared. The surface physicochemical properties, biomass attachment capacity and pollutants removal efficiency of MBF were systematically investigated. Electron microscope scanning (SEM) revealed that the surface roughness of BF was obviously improved by modification. Besides, fourier transform infrared (FTIR) suggested that the MBF had more surface-active functional groups. The results of sludge immobilization tests showed that PDA/PAM/MAH-BF had higher bio-affinity than ordinary BF with 1.5∼2.3 times on immobilization ratio of microorganisms (IRM). Furthermore, the performances of PDA/PAM/MAH-BF as biofilm carrier for pollutants were significantly higher than that of ordinary BF group. Among them, the highest removal efficiency of COD in PAD-BF biofilm reactor was 95.29 ± 0.99%, while that of BF group was 86.30 ± 3.09%. PAM-BF group had the best removal effect of nutrients with the removal efficiency of 90.83 ± 7.69% for TP and 91.25 ± 6.43% for TN, respectively, while the removal rate of BF group was only about 70%. The improvement of dissolved oxygen (DO) in the MBF reactors was consistent with the enhancement of contaminant removal. Therefore, PDA/PAM/MAH-BF can be used as promising biological carrier fillers in wastewater treatment engineering.


Assuntos
Eliminação de Resíduos Líquidos , Purificação da Água , Biofilmes , Reatores Biológicos , Silicatos , Eliminação de Resíduos Líquidos/métodos
17.
Ann Transl Med ; 9(15): 1241, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532378

RESUMO

BACKGROUND: N6-methyladenosine (m6A) RNA methylation regulators play crucial role in tumorigenicity and progression. However, their biological significance in primary glioblastomas (GBM) has not been fully elucidated. METHODS: In the present study, we evaluated the 22 m6A RNA regulators using the integrated data of primary GBM samples from The Cancer Genome Atlas and Chinese Glioma Genome Atlas databases. The different m6A modification patterns and m6A-related gene signature in primary GBM were distinguished by using principal component analysis. Single-sample gene set enrichment analysis was introduced to assess the relative level of immune infiltration. Gene set variation analysis was performed to calculate the enrichment score of the signaling pathways for different clusters. An m6A scoring scheme was established to evaluate the m6A modification pattern in individual tumors in order to predict prognosis and evaluate tumor microenvironment (TME) cell infiltration, immune response, and chemotherapy effect in primary GBM. RESULTS: Two distinct m6A modification subgroups associated with different clinical features and biological pathways were identified among the 371 primary GBM. Based on 132 prognostic m6A phenotype-related differentially expressed genes (DEGs) between 2 m6A cluster subgroups, an m6A scoring model was constructed to assess the m6A modification pattern in individual tumors. The high-m6A score group was associated with better prognosis and immune response and worse chemotherapy effect. CONCLUSIONS: The findings of the present study indicate the potential role of m6A modification in primary GBM, which will help enhance our understanding of TME characteristics, predict clinical prognosis, and provide important insight into effective immunotherapy and chemotherapy.

18.
BMC Genomics ; 22(1): 502, 2021 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-34217210

RESUMO

BACKGROUND: Dinotefuran (CAS No. 165252-70-0), a neonicotinoid insecticide, has been used to protect various crops against invertebrate pests and has been associated with numerous negative sublethal effects on honey bees. Long noncoding RNAs (lncRNAs) play important roles in mediating various biological and pathological processes, involving transcriptional and gene regulation. The effects of dinotefuran on lncRNA expression and lncRNA function in the honey bee brain are still obscure. RESULTS: Through RNA sequencing, a comprehensive analysis of lncRNAs and mRNAs was performed following exposure to 0.01 mg/L dinotefuran for 1, 5, and 10 d. In total, 312 lncRNAs and 1341 mRNAs, 347 lncRNAs and 1458 mRNAs, and 345 lncRNAs and 1155 mRNAs were found to be differentially expressed (DE) on days 1, 5 and 10, respectively. Gene set enrichment analysis (GSEA) indicated that the dinotefuran-treated group showed enrichment in carbohydrate and protein metabolism and immune-inflammatory responses such as glycine, serine and threonine metabolism, pentose and glucuronate interconversion, and Hippo and transforming growth factor-ß (TGF-ß) signaling pathways. Moreover, the DE lncRNA TCONS_00086519 was shown by fluorescence in situ hybridization (FISH) to be distributed mainly in the cytoplasm, suggesting that it may serve as a competing endogenous RNA and a regulatory factor in the immune response to dinotefuran. CONCLUSION: This study characterized the expression profile of lncRNAs upon exposure to neonicotinoid insecticides in young adult honey bees and provided a framework for further study of the role of lncRNAs in honey bee growth and the immune response.


Assuntos
RNA Longo não Codificante , Animais , Abelhas , Encéfalo , Guanidinas , Hibridização in Situ Fluorescente , Neonicotinoides , Nitrocompostos
19.
J Hazard Mater ; 413: 125295, 2021 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-33609865

RESUMO

Although the potential threats of metallic oxide nanoparticles (MNPs) to constructed wetland (CW) have been broadly reported, limited information is available regarding the long-term impact of nickel oxide nanoparticles (NiO NPs) on CWs at the environmentally relevant concentrations. Here, we comprehensively elucidated the responses in the treatment performance, enzyme activities, microbial properties, metabolic pathways and functional genes of CWs to chronic exposure of NiO NPs (0.1 and 1 mg/L) for 120 days, with a quantitative analysis on the fate and migration of NiO NPs within CWs. Nitrogen removal evidently declined under the long-term exposure to NiO NPs. Besides, NiO NPs induced a deterioration in phosphorus removal, but gradually restored over time. The activities of dehydrogenase (DHA), phosphatase (PST), urease (URE), ammonia oxygenase (AMO) and nitrate reductase (NAR) were inhibited to some extent under NiO NPs stress. Furthermore, NiO NPs exposure reduced bacterial diversity, shifted microbial composition and obviously inhibited the transcription of the ammonia oxidizing and denitrifying functional genes. The results of nickel mass balance indicated that the major removal mechanism of NiO NPs in CWs was through substrate adsorption and plants uptake. Thus, the ecological impacts of prolonged NiO NPs exposure at environmental concentrations should not be neglected.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Redes e Vias Metabólicas , Nanopartículas Metálicas/toxicidade , Nitrogênio , Fósforo , Eliminação de Resíduos Líquidos , Áreas Alagadas
20.
Clin Res Hepatol Gastroenterol ; 45(2): 101312, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33592427

RESUMO

BACKGROUND: The morbidity of nonalcoholic fatty liver disease (NAFLD) has increased consistently in recent years. Exenatide could reverse liver fibrosis and lower the occurrence of fatty liver. The aim of the study was to identify and characterize mRNA and miRNA expression to elucidate the mechanism of exenatide in the gerbil model. METHODS: Gerbils were fed a high-fat diet for 8 weeks to induce a fibrosis model; then, the gerbil models were treated with exenatide for 4 weeks. The total RNA extracted from the liver tissue samples was used to prepare the library and sequence on a HiSeq 2000. Bioinformatic methods were employed to analyze the sequence data to identify the mRNAs and miRNAs and to acquire the miRNA-mRNA regulatory network. RESULTS: By RNA-seq, 2344 differentially expressed genes (DEGs) and 72 miRNAs were found in the model group. Compared with the model group, 591 DEGs and 19 miRNAs were found in the quercetin group, whereas 876 DEGs and 18 miRNAs were found in the treatment group. The miRNA-mRNA regulatory network was constructed in a gerbil model. Immunohistochemistry and RNA sequencing confirmed that the therapeutic effect of exenatide may be derived from extrahepatic signal transduction. The key differential genes are CYP3A, CYP4A11, ACAA1, ACSM, PHX1, MAO, FMO, UGT, ACOX2, ABAT, PIK3C and PLCG1. The key miRNAs are miR-15a, miR-27b, miR-532-3P, miR-627, miR-3596, miR-142-3P, Let-7e-5p, miR-214-5, miR-101-3p, miR-378d. New miRNAs, such as novel_127, novel_143, novel_15, novel_204 are associated with liver fibrosis, while novel_127, novel_15, and novel_54 are associated with reverse treated with exenatide. CONCLUSIONS: Our research represents the first description of mRNA/miRNA profiles in a gerbil model of fatty liver fibrosis treated with exenatide, which may provide insights into the pathogenesis or treatment of the metabolic syndrome.


Assuntos
MicroRNAs , Hepatopatia Gordurosa não Alcoólica , Animais , Exenatida , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Gerbillinae , Humanos , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/tratamento farmacológico , Cirrose Hepática/genética , MicroRNAs/genética , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/genética , RNA Mensageiro
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