Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.360
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-34611980

RESUMO

Surface enhanced Raman scattering (SERS) based on chemical mechanism (CM) attracts tremendous attention for great selectivity and stability. However, low enhancement factor (EF) limits its practical applications for trace detection. Here, a novel sponge-like Cu-doping SnO2 -NiO p-n semiconductor heterostructure (SnO2 -NiOx/Cu), was first created as a CM-based SERS substrate with a significant EF of 1.46 × 1010 . This remarkable EF was mainly attributed to the enhanced charge-separation efficacy of p-n heterojunction and charge transfer resonance resulted from Cu doping. Moreover, the porous structure enriched the probe molecules, resulting in further SERS signals magnification. By immobilizing CuPc as an inner-reference element, SnO2 -NiOx/Cu was developed as a SERS nose for selective recognition of multiple lung cancer related VOCs down to ppb level. The information of VOCs was recorded in a barcode, demonstrating practical potential of a desktop SERS device for biomarker screening.

2.
Adv Mater ; : e2105541, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34613619

RESUMO

Lithium sulfur (Li-S) batteries have been considered as one of the most promising next-generation rechargeable batteries owing to its high energy density and cost-effectiveness. However, the sluggish kinetics of the sulfur reduction reaction process, which is so far insufficiently explored, still impedes its practical application. Metal organic frameworks (MOF) are widely investigated as sulfur immobilizer, but the interactions and catalytic activity of lithium polysulfides (LiPs) on metal nodes are weak due to the presence of organic ligands. Herein, we present a strategy to design Quasi-MOF nanospheres, which has a transition-state structure between MOF and metal oxide, via controlled ligand exchange strategy to serve as sulfur electrocatalyst. The Quasi-MOF not only inherits the porous structure of MOF, but also exposes abundant metal nodes to act as active sites, rendering strong LiPs absorbability. The reversible deligandation/ligandation of Quasi-MOF and its impact on the durability of catalyst over the course of electrochemical process has been acknowledged, which confers a remarkable catalytic activity. Attributed to these structural advantages, Quasi-MOF delivers a decent discharge capacity and low capacity fading rate over long-term cycling. This work not only offers insight into the rational design of Quasi-MOF based composite, but also provides guidance for the application in Li-S batteries. This article is protected by copyright. All rights reserved.

3.
ACS Omega ; 6(38): 25089-25095, 2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34604687

RESUMO

Four polyketide-amino acid derivatives, pardinumones A-D (1-4), were isolated from the wild mushroom Tricholoma pardinum. Their structures together with absolute configurations were characterized by means of spectroscopic data analyses, as well as calculated electronic circular dichroism (ECD) and NMR with sorted training set (STS) protocol analysis. Compounds 1-4 exhibited antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli with MIC values in the range of 6.25-50 µg/mL.

4.
Sensors (Basel) ; 21(19)2021 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-34640656

RESUMO

Image inpainting aims to fill in corrupted regions with visually realistic and semantically plausible contents. In this paper, we propose a progressive image inpainting method, which is based on a forked-then-fused decoder network. A unit called PC-RN, which is the combination of partial convolution and region normalization, serves as the basic component to construct inpainting network. The PC-RN unit can extract useful features from the valid surroundings and can suppress incompleteness-caused interference at the same time. The forked-then-fused decoder network consists of a local reception branch, a long-range attention branch, and a squeeze-and-excitation-based fusing module. Two multi-scale contextual attention modules are deployed into the long-range attention branch for adaptively borrowing features from distant spatial positions. Progressive inpainting strategy allows the attention modules to use the previously filled region to reduce the risk of allocating wrong attention. We conduct extensive experiments on three benchmark databases: Places2, Paris StreetView, and CelebA. Qualitative and quantitative results show that the proposed inpainting model is superior to state-of-the-art works. Moreover, we perform ablation studies to reveal the functionality of each module for the image inpainting task.


Assuntos
Processamento de Imagem Assistida por Computador , Redes Neurais de Computação
5.
Adv Mater ; : e2105904, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34664332

RESUMO

The separation efficiency of photo-generated carriers is still a great challenge that restricts the practical application of photocatalytic technology. The design of spatial separation path for photo-generated carriers at atomic level provides an innovative approach to address this challenge. Herein, a facile dual atomic sites strategy, consisting of Cu-N4 and C-S-C active moieties decorated on polymeric carbon nitride (Cu SAs/p-CNS) is reported to simultaneously achieve the highly efficient separation of photo-generated electrons and holes for boosting photocatalytic performance. As a proof of concept, the Cu SAs/p-CNS is successfully applied to the photo-oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-diformylfuran (DFF), which exhibits 77.1% HMF conversion and 85.6% DFF selectivity under visible light irradiation. The activity is considerably higher than that of bulk p-CN, S doped p-CN, and p-CN supported Cu single atom catalysts. Theoretical calculations and experimental results suggest that, during photocatalytic reaction, the isolated Cu-N4 sites directly capture photo-generated electrons, while the surrounding S atoms bear photo-generated holes, which synergistically facilitates the separation of photo-generated carriers and thus results in enhanced photocatalytic activity. This study provides a new perspective for the rational design of high performance photocatalysts at atomic level.

6.
An Acad Bras Cienc ; 93(suppl 3): e20210373, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34644724

RESUMO

Simazine was one of the most commonly used herbicides and was widely used to control broadleaf weeds in agriculture and forestry. Its widespread use had caused wide public concern for its high ecological toxicity. In order to remove simazine residues, 2 strains capable of effectively degrading simazine were isolated from the soil and named SIMA-N5 and SIMA-N9. SIMA-N5 was identified as Bacillus licheniformis by 16SrRNA sequence analysis, and SIMA-N9 was Bacillus altitudinis. According to the degradation ratio of simazine in a certain period of time, the degradation ability of different strains was evaluated. The degradation efficiency of simazine (5 mg/L) by SIMA-N9 could reach about 98% in 5d, and the strain SIMA-N5 could reach 94% under the same conditions. In addition, the addition of Pennisetum rhizosphere soil during the process of degrading simazine by strain SIMA-N9 could effectively improve the degradation efficiency. The strain SIMA-N9 has been developed as a microbial agent for the bioremediation of simazine contamination in soil. The new microbial agent developed by using SIMA-N9 has achieved satisfactory application effects. Based on the research results already obtained in this study, it was considered that strain SIMA-N9 and its live bacterial agent could play an important role in bioremediation of simazine pollution. This study could not only provide a set of solutions to the simazine pollution, but also provide a reference for the treatment of other pesticide pollution.


Assuntos
Herbicidas , Simazina , Bacillus , Bactérias/genética , Biodegradação Ambiental , Simazina/análise , Microbiologia do Solo
7.
Sci Total Environ ; : 151027, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34673057

RESUMO

Soil Cd pollution is a serious environmental issue associated with human activities. However, the factors determining exogenous Cd dynamics in the soil profile in a complex environment are not well understood. Based on regional observations from 169 soil profiles across the Chengdu Plain, this study explored the key factors controlling Cd accumulation in the soil profile under actual field conditions. Results showed that total soil Cd contents decreased from 0.377 to 0.196 mg kg-1 with increasing soil depth. The effects of phosphate fertilizer rates, road density and precipitation on the difference in total soil Cd content were only observed in topsoil, while agricultural land-use type and topography had no impact. In contrast, significant differences in the total soil Cd content among different parent material types were found in the 0-20, 40-60 and 60-100 cm soil depths. One sample t-tests showed that significant Cd accumulation occurred in the whole soil profile in soils formed from Q4 (Quaternary Holocene) grey alluvium, while soils formed from Q3 (Quaternary Pleistocene) old alluvium and Q4 grey-brown alluvium showed significant Cd accumulation only in the 0-40 cm soil layers. In the topsoil, acid soluble Cd accounted for the largest proportion of the total Cd in soils formed from Q4 grey alluvium, reducible Cd was the main fraction in soils formed from Q4 grey-brown alluvium, while reducible Cd and residual Cd contributed the largest proportion of the total soil Cd in soils formed from Q3 old alluvium. The above results indicated that parent material was the decisive factor determining the magnitudes and depths of exogenous Cd accumulation in the soil profile due to its impacts on the Cd fraction distributions. These findings suggested that the parent material-induced Cd fraction distributions and accumulation should be considered for effectively exploring targeted remediation strategies for Cd pollution.

8.
Front Cardiovasc Med ; 8: 670594, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595214

RESUMO

Purpose: To detect lung metastases, we conducted a retrospective study to improve patient prognosis. Methods: Hypertension patients with ocular metastases (OM group; n = 58) and without metastases (NM group; n = 1,217) were selected from individuals with lung cancer admitted to our hospital from April 2005 to October 2019. The clinical characteristics were compared by Student's t-test and chi-square test. Independent risk factors were identified by binary logistic regression, and their diagnostic value evaluated by receiver operating characteristic curve analysis. Results: Age and sex did not differ significantly between OM and NM groups; There were significant differences in pathological type and treatment. Adenocarcinoma was the main pathological type in the OM group (67.24%), while squamous cell carcinoma was the largest proportion (46.43%) in the NM group, followed by adenocarcinoma (34.10%). The OM group were treated with chemotherapy (55.17%), while the NM group received both chemotherapy (39.93%) and surgical treatment (37.06%). Significant differences were detected in the concentrations of cancer antigen (CA)-125, CA-199, CA-153, alpha fetoprotein (AFP), carcinoembryonic antigen (CEA), cytokeratin fraction 21-1 (CYFRA21-1), total prostate-specific antigen, alkaline phosphatase, and hemoglobin (Student's t-test). Binary logistic regression analysis indicated that CA-199, CA-153, AFP, CEA, and CYRFA21-1 were independent risk factors for lung cancer metastasis. AFP (98.3%) and CEA (89.3%) exhibited the highest sensitivity and specificity, respectively, while CYRFA21-1 had the highest area under the ROC curve value (0.875), with sensitivity and specificity values of 77.6 and 87.0%, respectively. Hence, CYFRA21-1 had the best diagnostic value.

9.
BMC Genomics ; 22(1): 714, 2021 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-34600494

RESUMO

BACKGROUND: As one of the largest genera in Apiaceae, Bupleurum L. is well known for its high medicinal value. The genus has frequently attracted the attention of evolutionary biologist and taxonomist for its distinctive characteristics in the Apiaceae family. Although some chloroplast genomes data have been now available, the changes in the structure of chloroplast genomes and selective pressure in the genus have not been fully understood. In addition, few of the species are endemic to Southwest China, a distribution and diversity center of Chinese Bupleurum. Endemic species are key components of biodiversity and ecosystems, and investigation of the chloroplast genomes features of endemic species in Bupleurum will be helpful to develop a better understanding of evolutionary process and phylogeny of the genus. In this study, we analyzed the sequences of whole chloroplast genomes of 4 Southwest China endemic Bupleurum species in comparison with the published data of 17 Bupleurum species to determine the evolutionary characteristics of the genus and the phylogenetic relationships of Asian Bupleurum. RESULTS: The complete chloroplast genome sequences of the 4 endemic Bupleurum species are 155,025 bp to 155,323 bp in length including a SSC and a LSC region separated by a pair of IRs. Comparative analysis revealed an identical chloroplast gene content across the 21 Bupleurum species, including a total of 114 unique genes (30 tRNA genes, 4 rRNA genes and 80 protein-coding genes). Chloroplast genomes of the 21 Bupleurum species showed no rearrangements and a high sequence identity (96.4-99.2%). They also shared a similar tendency of SDRs and SSRs, but differed in number (59-83). In spite of their high conservation, they contained some mutational hotspots, which can be potentially exploited as high-resolution DNA barcodes for species discrimination. Selective pressure analysis showed that four genes were under positive selection. Phylogenetic analysis revealed that the 21 Bupleurum formed two major clades, which are likely to correspond to their geographical distribution. CONCLUSIONS: The chloroplast genome data of the four endemic Bupleurum species provide important insights into the characteristics and evolution of chloroplast genomes of this genu, and the phylogeny of Bupleurum.


Assuntos
Apiaceae , Bupleurum , Genoma de Cloroplastos , Bupleurum/genética , China , Ecossistema , Filogenia
10.
Appl Opt ; 60(26): 7869-7877, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34613045

RESUMO

With the advantages of high velocity measurement accuracy and fast dynamic response, the laser Doppler velocimeter (LDV) is expected to replace the odometer to be combined with a strapdown inertial navigation system (SINS) to form a higher precision integrated navigation system. However, LDV scale factor error and misalignment angles between LDV and inertial measurement unit will affect the accuracy of navigation. Considering that not all global navigation satellite system (GNSS) receivers can directly provide velocity information and current mainstream calibration methods are sensitive to the measurement noise and outliers of velocity and position information, a robust calibration method aided by GNSS is proposed in this paper, which is based on position observation. Different from current popular calibration methods, the attitude information of the GNSS/SINS integrated navigation system obtained by an adaptive Kalman filter is used to construct the observation vector together with LDV velocity outputs and GNSS position outputs in this method. The LDV scale factor error and the misalignment angle are determined by the ratio of two observation vector modulus and the Davenport's q-method method, respectively. The accuracy and robustness of the calibration method are verified by one vehicle test with normal GNSS signals and one vehicle test with GNSS signals with outliers. And the horizontal position error of dead reckoning of the calibrated LDV/SINS integrated system are less than 0.0314% and 0.1033% of the mileage, respectively.

11.
Adv Mater ; : e2105951, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34617348

RESUMO

Zn metal anode has garnered growing scientific and industrial interest owing to its appropriate redox potential, low cost and high safety. Nevertheless, the instability of Zn anode caused by dendrite formation, hydrogen evolution and side reactions has greatly hampered its commercialization. Herein, an in situ grown ZnSe overlayer is crafted over one side of commercial Zn foil via chemical vapor deposition in a scalable manner, aiming to achieve optimized electrolyte/Zn interfaces with large-scale viability. Impressively, thus-derived ZnSe coating functions as a cultivator to guide oriented growth of Zn (002) plane at the infancy stage of stripping/plating cycles, thereby inhibiting the formation of Zn dendrites and the occurrence of side reactions. As a result, high cyclic stability (1530 h at 1.0 mA cm-2 /1.0 mAh cm-2 ; 172 h at 30.0 mA cm-2 /10.0 mAh cm-2 ) in symmetric cells is harvested. Meanwhile, when paired with V2 O5 based cathode, assembled full cell achieves an outstanding capacity (194.5 mAh g-1 ) and elongated lifespan (a capacity retention of 84% after 1000 cycles) at 5.0 A g-1 . Our reversible Zn anode enabled by the interfacial manipulation strategy via ZnSe cultivator is anticipated to satisfy the demand of commercial use. This article is protected by copyright. All rights reserved.

12.
Front Endocrinol (Lausanne) ; 12: 740902, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34621244

RESUMO

Fatty acid binding protein 4 (FABP4) has been associated with insulin resistance. Gestational diabetes mellitus (GDM) impairs fetal insulin sensitivity. Female newborns are more insulin resistant than male newborns. We sought to evaluate the association between GDM and cord blood FABP4, and explore potential sex dimorphic associations and the roles of sex hormones. This was a nested case-control study in the Shanghai Birth Cohort, including 153 pairs of newborns in GDM vs. euglycemic pregnancies matched by infant sex and gestational age at delivery. Cord plasma FABP4, leptin, total and high-molecular-weight adiponectin, testosterone and estradiol concentrations were measured. Adjusting for maternal and neonatal characteristics, cord plasma FABP4 (Mean ± SD: 27.0 ± 19.6 vs. 18.8 ± 9.6 ng/mL, P=0.045) and estradiol (52.0 ± 28.6 vs. 44.2 ± 26.6, ng/mL, P=0.005) concentrations were higher comparing GDM vs. euglycemic pregnancies in males, but similar in females (all P>0.5). Mediation analyses showed that the positive association between GDM and cord plasma FABP4 in males could be partly mediated by estradiol (P=0.03), but not by testosterone (P=0.72). Cord plasma FABP4 was positively correlated with total adiponectin in females (r=0.17, P=0.053), but the correlation was in the opposite direction in males (r=-0.11, P=0.16) (test for difference in r, P=0.02). Cord plasma FABP4 was not correlated with leptin in both sexes. The study is the first to demonstrate sex-dimorphic associations between GDM and cord plasma FABP4 or estradiol, and between FABP4 and adiponectin in newborns. GDM may affect fetal circulating FABP4 and estradiol levels in males only.

13.
Front Chem ; 9: 717210, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34660527

RESUMO

Cadmium is one of the most toxic heavy metal contaminants in soils and water bodies and poses a serious threat to ecosystems and humans. However, cadmium is also an important resource widely used in many industries. The recovery of cadmium in the form of high-value products is considered as an ideal disposal strategy for Cd-contaminated environments. In this work, Pistia stratiotes was used to recycle cadmium from wastewaters through phytoaccumulation and then transformed into carbon-supported cadmium sulfide photocatalyst (CdS@C) through carbonization and hydrothermal reaction. The CdS@C photocatalyst contained a mixture of cubic and hexagonal CdS with lower band gap energy (2.14 eV) and high electron-hole separation efficiency, suggesting an excellent photoresponse ability and photocatalytic efficiency. The impressive stability and photocatalytic performance of CdS@C were demonstrated in efficient photodegradation of organic pollutants. •OH and O2•- were confirmed as the major active species for organic pollutants degradation during CdS@C photocatalysis. This work provides new insights into addressing Cd contaminated water bodies and upcycling in the form of photocatalyst.

14.
Front Cardiovasc Med ; 8: 716916, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34676249

RESUMO

Objective: An effective clinical tool to assess endothelial function and arterial stiffness in patients with obstructive sleep apnea (OSA) is lacking. This study evaluated the clinical significance of subclinical markers for OSA management in males without serious complications. Patients/Methods: Males without serious complications were consecutively recruited. Clinical data, biomarker tests, reactive hyperemia index (RHI), and augmentation index at 75 beats/min (AIx75) measured by peripheral arterial tonometry were collected. An apnea hypopnea index (AHI) cutoff of ≥15 events/h divided the patients into two groups. Results: Of the 75 subjects, 42 had an AHI ≥15 events/h. Patients with an AHI ≥15 events/h had higher high-sensitivity C-reactive protein, tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor, and AIx75 values than the control group but no statistical difference in RHI was observed. After controlling for confounders, TNF-α was negatively correlated with the average oxygen saturation (r = -0.258, P = 0.043). RHI was correlated with the rapid eye movement (REM) stage percentage (r = 0.306, P = 0.016) but not with AHI (P > 0.05). AIx75 was positively correlated with the arousal index (r = 0.289, P = 0.023) but not with AHI (r = 0.248, P = 0.052). Conclusions: In males with OSA without severe complications, TNF-α and AIx75 are independently related to OSA. The role of RHI in OSA management requires further elucidation. These markers combined can comprehensively evaluate OSA patients to provide more evidence for the primary prevention of coronary heart disease and treatment response assessment.

15.
Water Sci Technol ; 84(5): 1146-1158, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34534112

RESUMO

A novel copper doped graphitic carbon nitride (Cu-C3N4) was successfully synthesized and used as an effective Fenton-like catalyst. Cu-C3N4 was characterized by scanning electron microscopy, surface area analyzer, Fourier transform infrared spectroscopy, X-ray diffractometer, and X-ray photoelectron spectroscopy. Effect of process parameters including catalyst dosage, hydrogen peroxide (H2O2) concentration, solution pH, and initial methylene blue (MB) concentration was investigated to evaluate catalytic performance. The pseudo first-order kinetic model was used to describe the catalytic process. The enhancement of MB degradation is observed assisted by ultrasound. MB degradation of 96% is obtained within 30 min in Cu-C3N4/H2O2/ultrasound system, and the corresponding rate constant is 0.099 min-1. Effective MB degradation is obtained over a broad pH range (3.3-9.9). The catalytic mechanism is examined by ultraviolet-visible spectra, quenching test, and electron spin resonance determination. The dominant mechanism of MB degradation is ascribed to the ultrasonic H2O2 activation by Cu-C3N4 for hydroxyl radical generation. Cu-C3N4 has good reusability and is effective to degrade rhodamine B and acid orange 7. This work not only contributes to the field of wastewater treatment, but also provides insights into the synthesis of Fenton-like catalysts. The results manifest that Cu-C3N4 is a promising Fenton-like catalyst for dye degradation in the field of environmental pollution remediation.


Assuntos
Corantes , Peróxido de Hidrogênio , Cobre , Grafite , Compostos de Nitrogênio
18.
Open Med (Wars) ; 16(1): 1318-1327, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34568579

RESUMO

As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 µmol/L P-A for 24 h; the release of H2S was the largest. After 100 µmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.

19.
ACS Appl Mater Interfaces ; 13(38): 45446-45454, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34533922

RESUMO

It is well known that the electrochemical performance of spinel LiMn2O4 can be improved by Al doping. Herein, combining X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, and spherical aberration-corrected scanning transmission electron microscopy (Cs-STEM) with in situ electron-beam (E-beam) irradiation techniques, the influence of Al doping on the structural evolution and stability improvement of the LiMn2O4 cathode material is revealed. It is revealed that an appropriate concentration of Al3+ ions could dope into the spinel structure to form a more stable LiAlxMn2-xO4 phase framework, which can effectively stabilize the surface and bulk structure by inhibiting the dissolution of Mn ions during cycling. The optimized LiAl0.05Mn1.95O4 sample exhibits a superior capacity retention ratio of 80% after 1000 cycles at 10 C (1 C = 148 mA h g-1) in the voltage range of 3.0-4.5 V, which possesses an initial discharge capacity of 90.3 mA h g-1. Compared with the undoped LiMn2O4 sample, the Al-doped sample also shows superior rate performance, especially the capacity recovery performance.

20.
Molecules ; 26(17)2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34500797

RESUMO

Despite significant advances in biological and analytical approaches, a comprehensive portrait of the proteome and its dynamic interactions and modifications remains a challenging goal. Chemical proteomics is a growing area of chemical biology that seeks to design small molecule probes to elucidate protein composition, distribution, and relevant physiological and pharmacological functions. Click chemistry focuses on the development of new combinatorial chemical methods for carbon heteroatom bond (C-X-C) synthesis, which have been utilized extensively in the field of chemical proteomics. Click reactions have various advantages including high yield, harmless by-products, and simple reaction conditions, upon which the molecular diversity can be easily and effectively obtained. This paper reviews the application of click chemistry in proteomics from four aspects: (1) activity-based protein profiling, (2) enzyme-inhibitors screening, (3) protein labeling and modifications, and (4) hybrid monolithic column in proteomic analysis.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...