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1.
BMC Cardiovasc Disord ; 23(1): 57, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36721091

RESUMO

BACKGROUND: Anti-mitochondrial antibody (AMA)-positive inflammatory myopathy, a rare type of idiopathic inflammatory myopathy which was frequently difficult to diagnose, can affect muscles and the structure and electrical conduction of the heart. Early identification and treatment of this myopathy can prevent serious cardiovascular adverse events and improve cardiac function. CASE PRESENTATION: We report a patient who experienced repeated syncope, ventricular tachycardia (VT) and heart failure accompanied by weakness and muscle atrophy. He was initially diagnosed with dilated cardiomyopathy and received implantable cardioverter-defibrillator therapy. He was subsequently misdiagnosed as muscular dystrophy due to progressive muscular atrophy. However, the patient developed repeated and refractory VT storms that were not alleviated by conventional therapy. Finally, he was diagnosed with AMA-positive inflammatory myopathy with cardiac injuries. The patient was markedly recovered by being treated with immunosuppressive and immunomodulatory therapy. CONCLUSION: AMA could be screened when discovering myopathies accompanied by unexplained cardiac symptoms. Our findings provide insights into the diagnosis and therapy of this rare and severe disease.

2.
BMC Plant Biol ; 23(1): 67, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36721119

RESUMO

BACKGROUND: Panax notoginseng (Burk) F.H. Chen is an essential plant in the family of Araliaceae. Its seeds are classified as a type of morphophysiological dormancy (MPD), and are characterized by recalcitrance during the after-ripening process. However, it is not clear about the molecular mechanism on the after-ripening in recalcitrant seeds. RESULTS: In this study, exogenous supply of gibberellic acid (GA3) with different concentrations shortened after-ripening process and promoted the germination of P. notoginseng seeds. Among the identified plant hormone metabolites, exogenous GA3 results in an increased level of endogenous hormone GA3 through permeation. A total of 2971 and 9827 differentially expressed genes (DEGs) were identified in response to 50 mg L-1 GA3 (LG) and 500 mg L-1 GA3 (HG) treatment, respectively, and the plant hormone signal and related metabolic pathways regulated by GA3 was significantly enriched. Weighted gene co-expression network analysis (WGCNA) revealed that GA3 treatment enhances GA biosynthesis and accumulation, while inhibiting the gene expression related to ABA signal transduction. This effect was associated with higher expression of crucial seed embryo development and cell wall loosening genes, Leafy Contyledon1 (LEC1), Late Embryogenesis Abundant (LEA), expansins (EXP) and Pectinesterase (PME). CONCLUSIONS: Exogenous GA3 application promotes germination and shorts the after-ripening process of P. notoginseng seeds by increasing GA3 contents through permeation. Furthermore, the altered ratio of GA and ABA contributes to the development of the embryo, breaks the mechanical constraints of the seed coat and promotes the protrusion of the radicle in recalcitrant P. notoginseng seeds. These findings improve our knowledge of the contribution of GA to regulating the dormancy of MPD seeds during the after-ripening process, and provide new theoretical guidance for the application of recalcitrant seeds in agricultural production and storage.

3.
Nat Commun ; 14(1): 36, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36596789

RESUMO

Two-dimensional (2D) van-der-Waals (vdW) layered ferroelectric semiconductors are highly desired for in-memory computing and ferroelectric photovoltaics or detectors. Beneficial from the weak interlayer vdW-force, controlling the structure by interlayer twist/translation or doping is an effective strategy to manipulate the fundamental properties of 2D-vdW semiconductors, which has contributed to the newly-emerging sliding ferroelectricity. Here, we report unconventional room-temperature ferroelectricity, both out-of-plane and in-plane, in vdW-layered γ-InSe semiconductor triggered by yttrium-doping (InSe:Y). We determine an effective piezoelectric constant of ∼7.5 pm/V for InSe:Y flakes with thickness of ∼50 nm, about one order of magnitude larger than earlier reports. We directly visualize the enhanced sliding switchable polarization originating from the fantastic microstructure modifications including the stacking-faults elimination and a subtle rhombohedral distortion due to the intralayer compression and continuous interlayer pre-sliding. Our investigations provide new freedom degrees of structure manipulation for intrinsic properties in 2D-vdW-layered semiconductors to expand ferroelectric candidates for next-generation nanoelectronics.

4.
Reprod Biol Endocrinol ; 21(1): 1, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36600238

RESUMO

BACKGROUND: The exact role of sperm reactive oxygen species (ROS) in early embryo development has yet to be fully identified, and most of existing research did not differentiate female infertility factors, ignoring the importance of oocyte quality in embryo development and the large differences in oocyte quality in women with infertility of different etiologies. And there has been no relevant report on whether different types of sperm ROS have distinct effects on embryo development. This study aimed to study the impact of selected sperm ROS, namely, sperm mitochondrial ROS (mROS) and hydrogen peroxide, on human embryo development after conventional in vitro fertilization (IVF) cycles in patients with normo-ovulatory infertility vs. anovulatory infertility. METHODS: This was a prospective investigation including 393 couples underwent IVF cycles, among whom 90 patients had anovulatory infertility and 303 patients had normo-ovulatory infertility in a public university-affiliated in vitro fertilization center. Sperm mROS and hydrogen peroxide testing were performed by flow cytometry and analyzed for their relationship with embryo development indices on days 1-6 after IVF. Multivariate logistic regression analysis was used to control for female potential confounders. The nonlinear effects of sperm ROS on embryo development were analyzed by the Restricted cubic spline (RCS) method. RESULTS: 1. Multivariate linear logistic regression analysis showed that high proportion of mROS positive sperm improved the 2PN rate (OR = 1.325, 95% CI: 1.103-1.595), day 3 embryo utilization rate (OR = 1.362, 95% CI: 1.151-1.614) and good-quality day 3 embryo rate (OR = 1.391, 95% CI: 1.089-1.783) in patients with anovulatory infertility. High percentage of sperm mROS and hydrogen peroxide had adverse effects on cleavage-stage embryo and blastocyst development in patients with normo-ovulatory infertility. 2. For patients with polycystic ovarian syndrome (PCOS) anovulatory infertility, there were significant distinct effects on embryo development indices between sperm mROS and hydrogen peroxide, and the increased rate of sperm mROS improved the good-quality day 3 embryo rate (OR = 1.435, 95% CI: 1.045-1.981); however, high percentage of sperm hydrogen peroxide reduced the blastocyst utilization rate (OR = 0.555, 95% CI: 0.353-0.864) and the good-quality blastocyst rate (OR = 0.461, 95% CI: 0.292-0.718). 3. Multivariate RCS analysis revealed that sperm ROS had a nonlinear (such as a parabolic curve) effect on embryo development in patients with anovulatory infertility (P < 0.05), and either greatly increased or greatly decreased affected cleavage-stage embryo and blastocyst development. The effects of sperm ROS in patients with normo-ovulatory infertility were both linear and nonlinear. CONCLUSIONS: These findings indicate that contrary effects of sperm mROS on embryo development depending on whether patients treated with IVF cycles had normal ovulation. Regardless of whether the patients ovulated normally, increased sperm hydrogen peroxide rate damaged blastocyst development. It is necessary to evaluate male sperm ROS levels and the female ovulatory state to determine an individualized intervention plan before starting cycles, as this may be beneficial for infertile couples.


Assuntos
Peróxido de Hidrogênio , Infertilidade Feminina , Humanos , Masculino , Feminino , Gravidez , Espécies Reativas de Oxigênio , Peróxido de Hidrogênio/farmacologia , Estudos Prospectivos , Sêmen , Fertilização In Vitro/métodos , Desenvolvimento Embrionário , Espermatozoides , Infertilidade Feminina/terapia , Taxa de Gravidez , Estudos Retrospectivos
5.
Artigo em Inglês | MEDLINE | ID: mdl-36604810

RESUMO

BACKGROUND: Prolapse is a common complication following enterostomy; the defect and consequences of a prolapse significantly affect health-related quality of life. Creative techniques must be employed to manage the prolapse. CASES: This article describes management of 3 neonates with stoma prolapse. CONCLUSION: Management of stoma prolapse should be individualized, employing successful nonoperative techniques rather than more difficult operative procedures to prevent recurrent prolapse.

6.
Am J Chin Med ; : 1-29, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36599648

RESUMO

Fibromyalgia (FM) is a complicated syndrome characterized by widespread chronic pain, fatigue, sleep disturbances, cognitive dysfunction, and other complications. There is currently no specific treatment available. No comprehensive surveys have been published to summarize the mechanism of acupuncture in FM management. Although several studies have shown that acupuncture can benefit FM patients, their clinical findings are inconsistent. Here, we summarize the operation method of acupuncture for FM. For the first time, we conducted a comprehensive review of the mechanisms of acupuncture for FM, and integrated evidence-based scientific findings with the most comprehensive and updated literature. According to studies conducted using FM patients and animal models, acupuncture may improve symptoms in FM patients by regulating the afferent pain pathway and descending inhibitory pain pathways of various molecules, such as ASIC3, Nav1.7, Nav1.8, and TRPV1, as well as peripheral inflammation and the autonomic nervous system. Furthermore, we discussed the epidemiology, pathophysiology, diagnosis, and management of FM, and reviewed acupuncture-related clinical studies. This review fills a previously unknown gap in knowledge of the mechanism of acupuncture for FM. Although there is growing evidence that acupuncture may be a promising therapy for treating symptoms in FM patients, further investigation is needed.

7.
Epigenomics ; 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36601794

RESUMO

EZH2 is an epigenetic regulator that methylates lysine 27 on histone H3 (H3K27) and is closely related to the development and metastasis of tumors. It often shows gain-of-function mutations in hematological tumors, while it is often overexpressed in solid tumors. EZH2 inhibitors have shown good efficacy in hematological tumors in clinical trials but poor efficacy in solid tumors. Therefore, current research on EZH2 inhibitors has focused on exploring additional combination strategies in solid tumors. Herein we summarize the combinations and mechanisms of EZH2 inhibitors and other therapies, including immunotherapy, targeted therapy, chemotherapy, radiotherapy, hormone therapy and epigenetic therapy, both in clinical trials and preclinical studies, aiming to provide a reference for better antitumor effects.


EZH2 is a histone methyltransferase whose high expression is associated with a poor prognosis in various solid tumors. It is considered a target for cancer treatment. However, EZH2 inhibitors as monotherapy have shown disappointing efficacy in most solid tumors. This review summarizes the strategies of combining EZH2 inhibitors with other treatment regimens and their associated molecular mechanisms.

9.
Nat Commun ; 14(1): 21, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36596763

RESUMO

Self-assembled systems have recently attracted extensive attention because they can display a wide range of phase morphologies in nanocomposites, providing a new arena to explore novel phenomena. Among these morphologies, a bicontinuous structure is highly desirable based on its high interface-to-volume ratio and 3D interconnectivity. A bicontinuous nickel oxide (NiO) and tin dioxide (SnO2) heteroepitaxial nanocomposite is revealed here. By controlling their concentration, we fabricated tuneable self-assembled nanostructures from pillars to bicontinuous structures, as evidenced by TEM-energy-dispersive X-ray spectroscopy with a tortuous compositional distribution. The experimentally observed growth modes are consistent with predictions by first-principles calculations. Phase-field simulations are performed to understand 3D microstructure formation and extract key thermodynamic parameters for predicting microstructure morphologies in SnO2:NiO nanocomposites of other concentrations. Furthermore, we demonstrate significantly enhanced photovoltaic properties in a bicontinuous SnO2:NiO nanocomposite macroscopically and microscopically. This research shows a pathway to developing innovative solar cell and photodetector devices based on self-assembled oxides.

10.
J Med Chem ; 66(2): 1601-1615, 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36634151

RESUMO

The protein N-terminal methyltransferase 1 (NTMT1) is implicated in neurogenesis, retinoblastoma, and cervical cancer. However, its pharmacological potentials have not been elucidated due to the lack of drug-like inhibitors. Here, we report the discovery of the first NTMT1 in vivo chemical probe GD433 by structure-guided optimization of our previously reported lead compound venglustat. GD433 (IC50 = 27 ± 1.1 nM) displays improved potency and selectivity than venglustat across biochemical, biophysical, and cellular assays. GD433 also displays good oral bioavailability and can serve as an in vivo chemical probe to dissect the pharmacological roles of Nα methylation. In addition, we also identified a close analogue (YD2160) that is inactive against NTMT1. The active inhibitor and negative control will serve as valuable tools to examine the physiological and pharmacological functions of NTMT1 catalytic activity.


Assuntos
Inibidores Enzimáticos , Metilação , Relação Estrutura-Atividade , Inibidores Enzimáticos/farmacologia
11.
J Pharm Biomed Anal ; 225: 115224, 2023 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-36603394

RESUMO

Xiaokeyinshui extract combination (XEC), originating from a traditional Chinese formula Xiaokeyinshui (XKYS) recorded in ancient Bencao, has been reported to exert significant hypoglycemic effects. However, the chemical profiles, metabolic transformation and pharmacokinetic behavior of XEC in vivo were unclear. The research was to investigate the chemical constituents, metabolic profiles and pharmacokinetic behavior of XEC. A UPLC-QE-Orbitrap-HRMS qualification method was developed to identify the chemical constituents in XEC and xenobiotics of XEC in plasma, urine, feces and bile of rats after oral administration. A LC-MS quantification method was established and applied for the pharmacokinetic studies of major active compounds of XEC in normal and T2DM rats and Coptidis Rhizoma extracts (CRE) in T2DM rats. Fifty eight compounds in XEC and a total of 152 xenobiotics were identified in T2DM rats, including 28 prototypes and 124 metabolites. The metabolic pathways were demethylation, demethyleneization, reduction, hydroxylation, hydrolysis and subsequent binding reactions, including glucuronidation, sulfation and methylation. According to the results of chemical constituents and metabolites, 7 ingredients, including berberine, palmatine, coptisine, epiberberine, berberrubine, magnoflorine and aurantio-obtusin were suggested for markers to comparative pharmacokinetics study in normal rats and T2DM rats. Compared with normal rats, the Tmax of berberine, palmatine, coptisine, epiberberine, berberrubine and magnoflorine was significantly longer. The value of Cmax for palmatine, coptisine, epiberberine and berberrubine was significantly decreased in XEC T2DM group. The value of AUC for alkaloids was higher in diabetic rats. After oral CRE, alkaloids including berberine, palmatine, coptisine, epiberberine, berberrubine and magnoflorine could be detected in vivo. Compared with T2DM rats after oral administration of CRE, the value of Tmax and Cmax for berberine, palmatine, coptisine, epiberberine, berberrubine and magnoflorine exhibited significant differences in XEC T2DM group. This research provided an overview of the chemical profiles and metabolic profiling of XEC and elucidated the effect of diabetic state and compatibility on pharmacokinetic behaviors of active components in XEC. This research also can provide the material basis of XEC for subsequent quality control research.


Assuntos
Alcaloides , Berberina , Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Medicamentos de Ervas Chinesas , Ratos , Animais , Xenobióticos , Alcaloides/química , Medicamentos de Ervas Chinesas/química
12.
Displays ; 77: 102370, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36644695

RESUMO

The Corona Virus Disease 2019 (COVID-19) has been declared a worldwide pandemic, and a key method for diagnosing COVID-19 is chest X-ray imaging. The application of convolutional neural network with medical imaging helps to diagnose the disease accurately, where the label quality plays an important role in the classification problem of COVID-19 chest X-rays. However, most of the existing classification methods ignore the problem that the labels are hardly completely true and effective, and noisy labels lead to a significant degradation in the performance of image classification frameworks. In addition, due to the wide distribution of lesions and the large number of local features of COVID-19 chest X-ray images, existing label recovery algorithms have to face the bottleneck problem of the difficult reuse of noisy samples. Therefore, this paper introduces a general classification framework for COVID-19 chest X-ray images with noisy labels and proposes a noisy label recovery algorithm based on subset label iterative propagation and replacement (SLIPR). Specifically, the proposed algorithm first obtains random subsets of the samples multiple times. Then, it integrates several techniques such as principal component analysis, low-rank representation, neighborhood graph regularization, and k-nearest neighbor for feature extraction and image classification. Finally, multi-level weight distribution and replacement are performed on the labels to cleanse the noise. In addition, for the label-recovered dataset, high confidence samples are further selected as the training set to improve the stability and accuracy of the classification framework without affecting its inherent performance. In this paper, three typical datasets are chosen to conduct extensive experiments and comparisons of existing algorithms under different metrics. Experimental results on three publicly available COVID-19 chest X-ray image datasets show that the proposed algorithm can effectively recover noisy labels and improve the accuracy of the image classification framework by 18.9% on the Tawsifur dataset, 19.92% on the Skytells dataset, and 16.72% on the CXRs dataset. Compared to the state-of-the-art algorithms, the gain of classification accuracy of SLIPR on the three datasets can reach 8.67%-19.38%, and the proposed algorithm also has certain scalability while ensuring data integrity.

13.
J Adv Nurs ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36694435

RESUMO

AIMS: This study aims to explore the correlation between fear of dementia and insomnia among community-dwelling older adults and to examine the mediating roles of social isolation and resilience on this correlation. METHODS: A total of 259 community-dwelling older adults from Mianyang, China were recruited from June 2021 to August 2021 using convenience sampling. The Chinese versions of the Fear of Dementia Scale (FODS), Lubben Social Network Scale (LSNS-6), 25-item Connor-Davidson Resilience Scale (CD-RISC-25) and Athens Insomnia Scale (AIS) were used to collect specific, study-related information from the subjects. Correlations between variables of interest were examined by Pearson analysis, and mediation analysis was conducted to explore the direct, indirect and total effects of the fear of dementia on insomnia vis-à-vis social isolation and resilience. RESULTS: Results from 259 older adults indicated that fear of dementia and insomnia in older adults are positively correlated, that social isolation and resilience mediate the relationship between them, and that social isolation and resilience also had a statistically significant serial mediating effect. CONCLUSION: Fear of dementia is positively related to insomnia in older community-dwelling Chinese adults, but resilience and social support may buffer the negative impact of fear of dementia and improve sleep quality. IMPACT: Fear of dementia is becoming more and more common in community-dwelling older adults in China, and this emotional response to ageing and disease anxiety may be to blame for the poor sleep quality of some ageing populations. However, social support and resilience may buffer the negative impact of fear of dementia. The findings in this study indicate a need for well-trained community nurses and other health practitioners to implement targeted strategies to reduce insomnia among older adults with fear of dementia. These strategies should strengthen resilience as well as social support.

14.
J Transl Med ; 21(1): 20, 2023 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-36635710

RESUMO

Recent studies have identified DNA replication stress as an important feature of advanced prostate cancer (PCa). The identification of biomarkers for DNA replication stress could therefore facilitate risk stratification and help inform treatment options for PCa. Here, we designed a robust machine learning-based framework to comprehensively explore the impact of DNA replication stress on prognosis and treatment in 5 PCa bulk transcriptomic cohorts with a total of 905 patients. Bootstrap resampling-based univariate Cox regression and Boruta algorithm were applied to select a subset of DNA replication stress genes that were more clinically relevant. Next, we benchmarked 7 survival-related machine-learning algorithms for PCa recurrence using nested cross-validation. Multi-omic and drug sensitivity data were also utilized to characterize PCa with various DNA replication stress. We found that the hyperparameter-tuned eXtreme Gradient Boosting model outperformed other tuned models and was therefore used to establish a robust replication stress signature (RSS). RSS demonstrated superior performance over most clinical features and other PCa signatures in predicting PCa recurrence across cohorts. Lower RSS was characterized by enriched metabolism pathways, high androgen activity, and a favorable prognosis. In contrast, higher RSS was significantly associated with TP53, RB1, and PTEN deletion, exhibited increased proliferation and DNA replication stress, and was more immune-suppressive with a higher chance of immunotherapy response. In silico screening identified 13 potential targets (e.g. TOP2A, CDK9, and RRM2) from 2249 druggable targets, and 2 therapeutic agents (irinotecan and topotecan) for RSS-high patients. Additionally, RSS-high patients were more responsive to taxane-based chemotherapy and Poly (ADP-ribose) polymerase inhibitors, whereas RSS-low patients were more sensitive to androgen deprivation therapy. In conclusion, a robust machine-learning framework was used to reveal the great potential of RSS for personalized risk stratification and therapeutic implications in PCa.


Assuntos
Neoplasias da Próstata , Masculino , Humanos , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/genética , Neoplasias da Próstata/metabolismo , Androgênios , Antagonistas de Androgênios/uso terapêutico , Aprendizado de Máquina , Replicação do DNA
15.
Eur J Nutr ; 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36717385

RESUMO

PURPOSE: To test the hypothesis that daily supplementation with low-dose B vitamins plus betaine could significantly reduce plasma homocysteine concentrations in Chinese adults with hyperhomocysteinemia and free from background mandatory folic acid fortification. METHODS: One hundred apparently healthy adults aged 18-65 years with hyperhomocysteinemia were recruited in South China from July 2019 to June 2021. They were randomly assigned to either the supplement group (daily supplementation: 400 µg folic acid, 8 mg vitamin B6, 6.4 µg vitamin B12 and 1 g betaine) or the placebo group for 12 weeks. Fasting venous blood was collected at baseline, week 4 and week 12 to determine the concentrations of homocysteine, folate, vitamin B12 and betaine. Generalized estimation equations were used for statistical analysis. RESULTS: Statistically significant increments in blood concentrations of folate, vitamin B12 and betaine after the intervention in the supplement group indicated good participant compliance. At baseline, there were no significant differences in plasma homocysteine concentration between the two groups (P = 0.265). After 12-week supplementation, compared with the placebo group, there was a significant reduction in plasma homocysteine concentrations in the supplement group (mean group difference - 3.87; covariate-adjusted P = 0.012; reduction rate 10.1%; covariate-adjusted P < 0.001). In the supplement group, the decreased concentration of plasma homocysteine was associated with increments of blood concentrations of both folate (ß = -1.680, P = 0.004) and betaine (ß = -1.421, P = 0.020) after 12 weeks of supplementation. CONCLUSIONS: Daily supplementation with low-dose B vitamins plus betaine for 12 weeks effectively decreased plasma homocysteine concentrations in Chinese adults with hyperhomocysteinemia. TRIAL REGISTRATION: This trial was registered at clinicaltrials.gov as NCT03720249 on October 25, 2018. Website: https://clinicaltrials.gov/ct2/show/NCT03720249 .

16.
Huan Jing Ke Xue ; 44(1): 494-501, 2023 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-36635837

RESUMO

Soil fungal community structure and diversity are highly sensitive to variations in the external environment, as well as soil improvement measures. In order to clarify the effects of soil improvement measures on topsoil fertility or quality, a field experiment was conducted in eroded forest of a red soil region. Organic fertilizer, biochar, and lime+microbial fertilizer were added to the topsoil, respectively. After four years, the chemistry properties and nutrients in the topsoil were measured, and the diversity and composition of fungi were analyzed. The results showed that the additions of organic fertilizer, biochar, and lime+microbial fertilizer reduced fungal richness in topsoil, compared to that with no fertilizer addition (CK). Among them, lime+microbial fertilizer had the most negative effect on fungal richness. The three soil improvement measures also affected the diversity of topsoil fungi, but the impacts were not significant. The dominant fungal phyla in the topsoil were Ascomycota (31.29%-46.55%) and Basidiomycota (30.07%-70.71%), and the dominant fungal genera were Amphinema and Archaeorhizomyces. The effects of soil improvement measures on fungal community structure in the topsoil were different; organic fertilizer increased the relative abundance of Ascomycetes and Archaeopteroides, and biochar enhanced the relative abundance of Basidiomycetes and Archaeopteroides, whereas lime+microbial fertilizer improved the relative abundance of Basidiomycetes and Archaeopteroides. Fungal diversity and community structure in the topsoil was affected by edaphic factors, and fungal richness was regulated by pH value, whereas fungal community structure was influenced by pH, total nitrogen, and organic carbon. This study provides scientific guidance for soil improvement and ecological restoration below the canopy in eroded forests of red soil regions.


Assuntos
Micobioma , Solo , Solo/química , Florestas , Microbiologia do Solo
17.
Pediatr Pulmonol ; 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36593123

RESUMO

BACKGROUND: Cystic fibrosis (CF)-related bone disease (CFBD) is seen in adults and can be associated with respiratory illness and malnutrition. There is limited and conflicting data regarding CFBD in pediatric CF. With longer life expectancy and promotion of disease prevention, pediatric CFBD demands further investigation. METHODS: Our center initiated a quality improvement (QI) project from April 2016 to December 2018 to improve CFBD screening in patients 8 years or older, per current CF Foundation (CFF) guidelines. Our team formulated a dual-energy X-ray absorptiometry (DXA) scan algorithm based upon degree of bone mineral density (BMD); shared CFBD guideline recommendations in our quarterly newsletter; and ordered scans for eligible patients at weekly review meetings. We reviewed DXA results from 141 patients after institutional review board approval and gathered data including comorbidities, genetics, anthropometric measures, medication exposure, and relevant serum studies. RESULTS: Fifty-three percent of our patients had normal BMD (n = 75). Seventeen patients (12%) had a Z score ≤ -2. Patients with lower BMD also had lower mean forced expiratory volume (FEV1 ) percent predicted (FEV1 %) (p < 0.001) as well as lower body mass index % (p = 0.001). Patients with lower BMD were overall older at time of DXA (p = 0.016). During study duration, 13 patients who had abnormal DXA results underwent repeat DXAs after physical therapy; 11 of the 13 showed improvement in DXA results. CONCLUSIONS: A DXA scan is a useful screening tool and can be used to identify pediatric patients who could benefit from further therapy and interventions to preserve adequate bone health and avoid further loss. QI initiatives can lead to improved screening and diagnosis and earlier intervention such as physical therapy. Further studies are needed to better understand the utility of physical therapy in children with CF.

18.
J Med Internet Res ; 25: e39786, 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36652280

RESUMO

BACKGROUND: Intelligent physical robots based on artificial intelligence have been argued to bring about dramatic changes in health care services. Previous research has examined the use of intelligent physical robots in the health care context from different perspectives; however, an overview of the antecedents and consequences of intelligent physical robot use in health care is lacking in the literature. OBJECTIVE: In this paper, we aimed to provide an overview of the antecedents and consequences of intelligent physical robot use in health care and to propose potential agendas for future research through a systematic literature review. METHODS: We conducted a systematic literature review on intelligent physical robots in the health care field following the guidelines of PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). Literature searches were conducted in 5 databases (PubMed, Scopus, PsycINFO, Embase, and CINAHL) in May 2021, focusing on studies using intelligent physical robots for health care purposes. Subsequently, the quality of the included studies was assessed using the Mixed Methods Appraisal Tool. We performed an exploratory content analysis and synthesized the findings extracted from the included articles. RESULTS: A total of 94 research articles were included in the review. Intelligent physical robots, including mechanoid, humanoid, android, and animalistic robots, have been used in hospitals, nursing homes, mental health care centers, laboratories, and patients' homes by both end customers and health care professionals. The antecedents for intelligent physical robot use are categorized into individual-, organization-, and robot-related factors. Intelligent physical robot use in the health care context leads to both non-health-related consequences (emotional outcomes, attitude and evaluation outcomes, and behavioral outcomes) and consequences for (physical, mental, and social) health promotion for individual users. Accordingly, an integrative framework was proposed to obtain an overview of the antecedents and consequences of intelligent physical robot use in the health care context. CONCLUSIONS: This study contributes to the literature by summarizing current knowledge in the field of intelligent physical robot use in health care, by identifying the antecedents and the consequences of intelligent physical robot use, and by proposing potential future research agendas in the specific area based on the research findings in the literature and the identified knowledge gaps.


Assuntos
Inteligência Artificial , Robótica , Humanos , Robótica/métodos , Atitude , Pessoal de Saúde/psicologia , Atenção à Saúde
19.
Bioresour Technol ; 369: 128464, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36509308

RESUMO

In this study, a renewable organic acid (xylonic acid), which can be prepared by the biooxidation of xylose, is used for pretreating sugarcane bagasse. The effects of reaction temperature and time on the release of fermentable xylose and glucose were investigated. On the basis of guaranteeing the good enzymatic hydrolysis efficiency and minimizing the effects of inhibitors, the pretreatment with 1 % xylnoic acid at 190 °C for 30 min was selected after optimization. In this case, 70 % xylose was released, while enzymatic hydrolysis yield was also up to 86.5 %. Meanwhile, the pretreated hydrolysate liquor was proved that it could be used for producing xylonate by biooxidation of Gluconobacter oxydans. Finally, the sequential process of the xylonic acid pretreatment and saccharification also clear the path for recycling the lignin as value-added bioproducts. Overall, this study presents a green-like strategy for fully exploiting sugarcane bagasse.


Assuntos
Celulose , Saccharum , Xilose , Açúcares , Hidrólise
20.
Appl Microbiol Biotechnol ; 107(2-3): 543-552, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36504328

RESUMO

While previous studies mainly focused on the total number of spores as an index to predict the calcium precipitation activity (CPA) of bacterial strains, the effect of viability of spores on microbial-induced calcium precipitation (MICP) has remained highly ignored. Therefore, for the first time, we have attempted to optimize the sporulation process in terms of viable spore production and, most importantly, aimed to build a correlation between viable spores and CPA. The results have shown that for the sporulation of Bacillus sp. H4, starch and peptone are the optimal carbon and nitrogen sources, respectively. One gram per liter of sodium chloride promotes CPA and production of viable spores, whereas an increase of sodium chloride concentration beyond 8 g L-1 significantly reduces CPA without reducing the quantity of viable spores. Exogenous conditions such as seed age, inoculation quantity, and liquid volume only pose slight influence on the sporulation and CPA. Conclusively, the spores produced under optimized conditions are more morphologically uniform and display a 20% increase in CPA compared to pre-optimized spores. Furthermore, by combining the results of heatmap analysis, it can be concluded that not only the quantity, but also the quality of viable spores is important for bacterial strain to develop high CPA and effective MICP process. This study sheds light on the breadth of biomineralization activity based on viable spores and is an imperative step toward the intelligible design of MICP-based engineering solutions. KEY POINTS: • Viability of spores is a key controlling factor in calcium precipitation activity (CPA). • Spores produced under optimized conditions display a 20% increase in CPA. • Quality of viable spores is imperative for bacterial strains to develop high CPA.


Assuntos
Bacillus , Carbonato de Cálcio , Cálcio , Cloreto de Sódio , Esporos Bacterianos , Bactérias , Precipitação Química , Viabilidade Microbiana
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