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1.
Int J Mol Med ; 45(4): 1187-1194, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32124954

RESUMO

Chronic myeloid leukemia (CML) is a myeloproliferative disorder that accounts for ~10% of all newly diagnosed leukemia cases. Early diagnosis is essential for long­term beneficial outcomes. The present study observed that interferon­induced protein with tetratricopeptde repeats 2 (IFIT2) expression levels were reduced in bone marrow samples from CML patients compared with control samples using RNA sequencing and reverse transcription­PCR. IFIT2 expression levels were restored in patients treated with tyrosine kinase inhibitors. To investigate the effect of IFIT2 on CML patients, a stable IFIT2 expressing K562 cell line was established. It was demonstrated that IFIT2 overexpression in K562 cells inhibits cell proliferation and arrests the cell cycle at the G1 phase. In addition, it was demonstrated by western blotting that IFIT2 inhibits the BCR­ABL oncoprotein and regulates its downstream AKT/mTOR signaling pathway. IFIT2 could induce cell cycle arrest­associated gene p27kip1 by degrading cullin1­mediated E3 ligases. In summary, the present study demonstrated that IFIT2 was efficacious in inhibiting CML and is a potential therapeutic target.

2.
BMC Pulm Med ; 20(1): 68, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32197604

RESUMO

BACKGROUND: Chronic cough has an important impact on physical, social and psychological aspects. A simple and effective method to assess different aspects of chronic cough severity is required. We aimed to develop a simple, self-completed test, Cough Evaluation Test (CET), to evaluate cough severity and its impact on health. METHOD: The items of preliminary CET were generated based on literature review and clinical practice. Items reduction was conducted by modified Delphi method. Patients with chronic cough were recruited to complete CET, Cough Visual Analog Scales (VAS), Mandarin Chinese version of the Leicester Cough Questionnaire (LCQ-MC), and Cough Symptom Score (CSS). Reassessments were performed at 1 week apart before treatment, and after more than 2 weeks treatments. Concurrent validation, internal consistency, repeatability, responsiveness and the minimal important difference (MID) were determined. RESULTS: CET consists of five items with a 5-point Likert scale (1-5 scaling of items, 5-25 score range). The Cronbach's alpha values for CET was 0.80. CET showed a stronger correlation with LCQ-MC (r = - 0.74) compared to that between LCQ-MC with VAS (r = - 0.61). CET also showed a stronger correlation with VAS (r = 0.70) compared to that between VAS with other measures. Intraclass correlation coefficients for CET was 0.84. In patients undergoing treatment, CET scores significantly changed (p < 0.0001). The MID of CET was 2. CONCLUSION: Cough Evaluate Test is a reliable, valid and responsive tool to simply evaluate impact of cough on physical, social and psychological aspects.

3.
Clin Cancer Res ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32193228

RESUMO

PURPOSE: Nintedanib enhances the activity of chemotherapy in metastatic NSCLC. In this phase I/II study, we assessed safety and efficacy of nintedanib plus neoadjuvant chemotherapy, using major pathologic response (MPR) as primary endpoint. EXPERIMENTAL DESIGN: Eligible patients had stage IB (≥4 cm)-IIIA resectable NSCLC. A safety run-in phase was followed by an expansion phase with nintedanib 200 mg PO bid (28 days), followed by 3 cycles of cisplatin (75 mg/m2), docetaxel (75 mg/m2) q21 days plus nintedanib, followed by surgery. With 33 planned patients, the study had 90% power to detect an MPR increase from 15% to 35%. RESULTS: 21 patients (stages I/II/III, N=1/8/12) were treated. One of 15 patients treated with nintedanib 200 mg achieved MPR (7%, 95% CI 0.2%-32%). Best ORR in 20 evaluable patients was 30% (6/20, 95% CI 12%-54%). 12-month RFS and OS were 66% (95% CI 47%-93%) and 91% (95% CI, 79%-100%), respectively. Most frequent treatment-related G3-4 toxicities were transaminitis and electrolyte abnormalities. Based on an interim analysis the study was discontinued for futility. Higher levels of CD3+ and cytotoxic CD3+CD8+ T cells were found in treated tumors of patients who were alive than in those who died (652.8 vs. 213.4 cells/mm2, P=0.048; 142.3 vs. 35.6 cells/mm2, P=0.018). CONCLUSIONS: Although tolerated, neoadjuvant nintedanib plus chemotherapy did not increase MPR rate compared to chemotherapy historical controls. Additional studies of the combination in this setting are not recommended. Post-treatment levels of tumor infiltrating T cells were associated with patient survival. Use of MPR facilitates the rapid evaluation of neoadjuvant therapies.

4.
J Immunol ; 204(7): 1736-1745, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32111731

RESUMO

IL-37 is a newly identified immune-suppressive factor; however, the function, cellular sources, and mechanism of IL-37 in humoral immunity and Myasthenia gravis (MG) are still unclear. In this study, we found IL-37 were substantially downregulated in the serum and PBMCs of MG patients compared with healthy controls. The lower IL-37 was associated with severer disease (quantitative MG score) and higher follicular Th (Tfh)/Tfh17 and B cell numbers. Flow cytometry analysis revealed that IL-37 was mainly produced by CD4+ T cells without overlapping with Th1, Th17, and Tfh subsets in MG patients. Regulatory IL-37+ T cell rarely expressed Foxp3 and CD25 but produced numerous IL-4. Tfh and B cell expressed high levels of SIGIRR, the receptor of IL-37, in MG patients. Mechanically, IL-37 directly bond to SIGIRR, repressed the proliferation, cytokine production of Tfh and B cells, and the secretion of autoantibody via inhibition of STAT3 signaling in Tfh and B cells.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32189200

RESUMO

In order to learn about the status of heavy metals and ecological risks in the water of thirteen China's costal shellfish culture areas, principal component analysis was applied to analyze the distributions of five kinds of heavy metal, while the safety threshold method was employed to evaluate the ecological risks. The results showed that among the 13 shellfish culture areas, the average metal concentrations were in the order of Zn > Cu > Pb > Hg > Cd, and all heavy metals were below the second standards for sea water quality of China. The distribution of heavy metals in 13 shellfish culture areas was reflected by two principal components with the contribution rates of F1(Cu) and F2(Zn, Pb) were 36.14% and 23.40%,respectively,which was less affected by economic and geographical differences. The ecological risks assessment of five kinds of heavy metals showed that MOS10 was more than one; the probabilities of five heavy metals affecting more than 10% of aquatic organisms were 49.52%, 24.11%, 17.49%, 22.05%, and 5.66%, respectively, the ecological risk of five main heavy metals was in order Cu > Zn > Cd > Pb > Hg. In the selected thirteen shellfish culture areas, the value of msPAF of seven culture areas was higher than 20%, indicating these areas are highly ecologically contaminated by heavy metals, and the other four shellfish culture areas with value of 0, indicating that these areas have no ecological risk of heavy metals pollution.

6.
Arch Pathol Lab Med ; 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32150457

RESUMO

CONTEXT.­: Identification of gene mutations that are indicative of epithelial-mesenchymal transition and a noninflammatory immune phenotype may be important for predicting response to immune checkpoint inhibitors. OBJECTIVE.­: To evaluate the utility of multiplex immunofluorescence for immune profiling and to determine the relationships among tumor immune checkpoint and epithelial-mesenchymal transition genomic profiles and the clinical outcomes of patients with nonmetastatic non-small cell lung cancer. DESIGN.­: Tissue microarrays containing 164 primary tumor specimens from patients with stages I to IIIA non-small cell lung carcinoma were examined by multiplex immunofluorescence and image analysis to determine the expression of programmed death ligand-1 (PD-L1) on malignant cells, CD68+ macrophages, and cells expressing the immune markers CD3, CD8, CD57, CD45RO, FOXP3, PD-1, and CD20. Immune phenotype data were tested for correlations with clinicopathologic characteristics, somatic and germline genetic variants, and outcome. RESULTS.­: A high percentage of PD-L1+ malignant cells was associated with clinicopathologic characteristics, and high density of CD3+PD-1+ T cells was associated with metastasis, suggesting that these phenotypes may be clinically useful to identify patients who will likely benefit from immunotherapy. We also found that ZEB2 mutations were a proxy for immunologic ignorance and immune tolerance microenvironments and may predict response to checkpoint inhibitors. A multivariate Cox regression model predicted a lower risk of death for patients with a high density of CD3+CD45RO+ memory T cells, carriers of allele G of CTLA4 variant rs231775, and those whose tumors do not have ZEB2 mutations. CONCLUSIONS.­: Genetic variants in epithelial-mesenchymal transition and immune checkpoint genes are associated with immune cell profiles and may predict patient outcomes and response to immune checkpoint blockade.

7.
Exp Eye Res ; 193: 107949, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-32006561

RESUMO

Retinal detachment (RD) results in disruption of retinal physiology and visual function. Although surgical intervention has been well-developed to restore the retinal anatomic structure, post-op progression of visual function decline is prominent in a large proportion of patients. Therefore, the establishment of a disease model that accurately mimics RD pathogenesis is crucial to mechanistic study and drug screening. General protocols to induce RD in mice are frequently associated with complications leading to model instability and reduced reproducibility. In this study, we established a stable and reproducible mice RD model with a detached area of over 90% and rare complications. Briefly, the modified method was realized by vitreous humor extraction to reduce intraocular pressure, followed by directly-visible hyaluronic acid injection into subretinal space. The detachment of retina was confirmed by fundus photography, and progressive thinning of the outer nuclear layer (ONL) was determined by HE staining. Apoptotic signals were prominent in the ONL. Consistently, visual function was significantly compromised as determined by ERG. Moreover, retinal vasculature appeared to remodel and acquired winding, twisted and dilated structures illustrated by 3D reconstruction. In addition, activation of Müller cells and microglia, and infiltration of blood-derived macrophages were detected locally. Collectively, we have established a modified protocol to model RD with increased stability, reproducibility and fewer complications, and 3D high-resolution imaging and reconstruction of vasculature could provide new tools to evaluate this model.

8.
J Neuroinflammation ; 17(1): 65, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32075657

RESUMO

BACKGROUND: Alzheimer's disease (AD) is a neurodegenerative disorder strongly correlated with a dysfunctional immune system. Our previous results demonstrated that inactivated influenza vaccine (IIV) facilitates hippocampal neurogenesis and blocks lipopolysaccharide (LPS)-induced cognitive impairment. However, whether IIV improves cognitive deficits in an AD mouse model remains unclear. In addition, early interventions in AD have been encouraged in recent years. Here, we investigated whether IIV immunization at the preclinical stage of AD alters the brain pathology and cognitive deficits in an APP/ PS1 mouse model. METHODS: We assessed spatial learning and memory using Morris water maze (MWM). The brain ß-amyloid (Aß) plaque burden and activated microglia were investigated by immunohistochemistry. Furthermore, flow cytometry was utilized to analyze the proportions of Treg cells in the spleen. A cytokine antibody array was performed to measure the alteration of cytokines in the brain and peripheral immune system. RESULTS: Five IIV immunizations activated microglia, reduced the Aß burden and improved the cognitive impairment. Simultaneously, the IIV-induced immune response broke peripheral immunosuppression by reducing Foxp3+ regulatory T cell (Treg) activities, whereas the restoration of Treg level in the periphery using all-trans retinoic acid (ATRA) blunted the protective effects of IIV on Aß burden and cognitive functions. Interestingly, IIV immunization might increase proinflammatory and anti-inflammatory cytokine expression in the brain of APP/PS1 mice, enhanced microglial activation, and enhanced the clustering and phagocytosis of Aß, thereby creating new homeostasis in the disordered immune microenvironment. CONCLUSIONS: Altogether, our results suggest that early multiple IIV immunizations exert a beneficial immunomodulatory effect in APP/PS1 mice by breaking Treg-mediated systemic immune tolerance, maintaining the activation of microglia and removing of Aß plaques, eventually improving cognitive deficits.

9.
Nanoscale ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31994577

RESUMO

The development of a nonprecious and Earth-abundant electrocatalyst with high electrocatalytic activity for the oxygen evolution reaction (OER) is an emerging hot issue and remains a grand challenge. In the present work, we proposed a facile strategy to construct ultrathin NiO nanosheets decorated with Fe-V nanoparticles on nickel foam (Fe-V@NiO/NF) for use as an OER electrocatalyst. Due to the 3D rational configuration, the Fe-V@NiO/NF with a heterostructure shows excellent electrocatalytic activity towards the OER. Interestingly, it is found that in situ oxidation by galvanostatic electrolysis in alkaline solution is beneficial to enhance the OER performance. After 10 h of electrolysis, a current density of 50 mA cm-2 is achieved at a low overpotential of 271.1 mV. This is because during the in situ oxidation process, iron and vanadium ions insert into the NiO lattice and lead to the generation of highly active α-FeOOH and an amorphous (oxy)-hydroxide layer. Additionally, the charge transfer resistance dramatically reduces with the prolonging of oxidation time.

10.
PLoS One ; 15(1): e0227610, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31990944

RESUMO

Atractylodes lancea, A. chinensis, and A. macrocephala are the three most widely used medicinal species of the Atractylodes genus. Their similar morphological features cause disagreement as whether they are three unique species, leading to their frequent misuses in medical products. Our study aimed to understand their relationships through both the complete plastome sequences and nuclear sequences, to identify molecular markers for their differentiation and explore the evolutionary relationships among three species. We sequenced, annotated, and analyzed the plastomes of these three species. The plastomes are 153,201, 153,258, and 153,265 bps in length for A. lancea, A. chinensis, and A. macrocephaly, respectively. Similar to other Asteraceae species, their plastomes exhibit typical quadripartite structures. Each plastome consists of 119 distinct genes, including 78 protein-coding, 37 tRNA, and 4 rRNA genes. Analyses of indels, single-nucleotide polymorphisms and simple sequence repeats, and comparison of plastomes showed high degree of conservation, leading to difficulty in the discovery of differentiating markers. We identified eleven potential molecular markers using an algorithm based on interspecific and intraspecific nucleotide diversity gaps. Validation experiments with fifty-five individuals from the three species collected from the botanical garden and fields confirmed that the marker cz11 could effectively distinguish samples from the three different species. Analysis of the several nuclear sequences suggests that the species of A. macrocephala may be a hybrid of A. lancea and A. chinensis. In summary, the results from this study highlight the complex relationships among of these three medicinal plants.

11.
Mol Ther ; 28(3): 855-873, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-31991111

RESUMO

Mesenchymal stromal cells (MSCs) show potential for treating cardiovascular diseases, but their therapeutic efficacy exhibits significant heterogeneity depending on the tissue of origin. This study sought to identify an optimal source of MSCs for cardiovascular disease therapy. We demonstrated that Nestin was a suitable marker for cardiac MSCs (Nes+cMSCs), which were identified by their self-renewal ability, tri-lineage differentiation potential, and expression of MSC markers. Furthermore, compared with bone marrow-derived MSCs (Nes+bmMSCs) or saline-treated myocardial infarction (MI) controls, intramyocardial injection of Nes+cMSCs significantly improved cardiac function and decreased infarct size after acute MI (AMI) through paracrine actions, rather than transdifferentiation into cardiac cells in infarcted heart. We further revealed that Nes+cMSC treatment notably reduced pan-macrophage infiltration while inducing macrophages toward an anti-inflammatory M2 phenotype in ischemic myocardium. Interestingly, Periostin, which was highly expressed in Nes+cMSCs, could promote the polarization of M2-subtype macrophages, and knockdown or neutralization of Periostin remarkably reduced the therapeutic effects of Nes+cMSCs by decreasing M2 macrophages at lesion sites. Thus, the present work systemically shows that Nes+cMSCs have greater efficacy than do Nes+bmMSCs for cardiac healing after AMI, and that this occurs at least partly through Periostin-mediated M2 macrophage polarization.

12.
Cancer Biol Ther ; 21(4): 309-314, 2020 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-31959056

RESUMO

Acute promyelocytic leukemia (APL) is characterized by the presence of promyelocytic leukemia-retinoic acid receptor α (PML-RARα) fusion gene, which is formed following the specific chromosomal translocation t(15;17)(q22;q21). However, cases with PML-RARα generated by occult t(15;17) which are negative by both cytogenetics and fluorescence in situ hybridization (FISH), are difficult to diagnose, leading to impaired treatment effectiveness. In the present study, we reported a case of a 66-year-old male patient, and bone marrow morphology, flow cytometry and cytogenetics did not support the diagnosis of APL. Molecular techniques, such as reverse-transcription polymerase chain reaction (RT-PCR), showed the existence of a cryptic PML-RARα fusion gene, and sequence analysis revealed a new variable isoform. Hotspot gene mutation analysis showed a biallelic CEBPA mutation. He received IA chemotherapy and all-trans retinoic acid (ATRA) treatment, and finally achieved complete remission. This case report provided valuable insights into the relevance of the correct identification of atypical PML-RARα fusion gene and biallelic CEBPA mutation. Moreover, combination of IA chemotherapy and ATRA treatment suggested a good clinical effect in this atypical PML-RARα.

13.
Carbohydr Polym ; 230: 115593, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31887873

RESUMO

The aim of this study was to investigate the in vitro digestion and fermentation prebiotic properties of three released-exopolysaccharide fractions (r-EPS1, r-EPS2 and r-EPS3) from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1. There were no free oligosaccharides and/or monosaccharides for r-EPS1 before and after simulated buccal, gastric and small intestinal (GSI) digestion in vitro. In contrast, r-EPS2 (13.4 %) and r-EPS3 (10.6 %) generated a few monosaccharides after digestion. Additionally, r-EPS1 and r-EPS2 seemed to present a strong bifidogenic effect comparing to inulin, as they exhibited high values of selectivity index (13.17 and 12.84, respectively). Furthermore, the fermentation with r-EPS1 produced the highest contents of acetic acid and lactic acid (56.3 mM and 44.29 mM, respectively), which resulted in the highest amounts of total short chain fatty acid (145.51 mM) followed by r-EPS2 (135.57 mM) and inulin (99.28 mM). These results indicated that r-EPS from L. delbrueckii ssp. bulgaricus SRFM-1 could be a good potential candidate for new functional food prebiotic.

15.
G3 (Bethesda) ; 10(1): 129-141, 2020 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-31672849

RESUMO

Autophagy is a catabolic process that provides cells with energy and molecular building blocks during nutritional stress. Autophagy also removes misfolded proteins and damaged organelles, a critical mechanism for cellular repair. Earlier work demonstrated that heparan sulfate proteoglycans, an abundant class of carbohydrate-modified proteins found on cell surfaces and in the extracellular matrix, suppress basal levels of autophagy in several cell types during development in Drosophila melanogaster In studies reported here, we examined the capacity of heparan sulfate synthesis to influence events affected by autophagy, including lifespan, resistance to reactive oxygen species (ROS) stress, and accumulation of ubiquitin-modified proteins in the brain. Compromising heparan sulfate synthesis increased autophagy-dependent processes, evident by extended lifespan, increased resistance to ROS, and reduced accumulation of ubiquitin-modified proteins in the brains of ROS exposed adults. The capacity of altering heparan sulfate biosynthesis to protect cells from injury was also evaluated in two different models of neurodegeneration, overexpression of Presenilin and parkin mutants. Presenilin overexpression in the retina produces cell loss, and compromising heparan sulfate biosynthesis rescued retinal patterning and size abnormalities in these animals. parkin is the fly homolog of human PARK2, one of the genes responsible for juvenile onset Parkinson's Disease. Parkin is involved in mitochondrial surveillance and compromising parkin function results in degeneration of both flight muscle and dopaminergic neurons in Drosophila Altering heparan sulfate biosynthesis suppressed flight muscle degeneration and mitochondrial dysmorphology, indicating that activation of autophagy-mediated removal of mitochondria (mitophagy) is potentiated in these animals. These findings provide in vivo evidence that altering the levels of heparan sulfate synthesis activates autophagy and can provide protection from a variety of cellular stressors.

16.
Huan Jing Ke Xue ; 40(7): 3313-3323, 2019 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854733

RESUMO

In order to explore the effect of land-use change on soil bacteria in wetland systems, the topsoil (0-20 cm) of a natural wetland (NW), paddy field (PF), and dry land (DL) were collected in the Huixian karst wetland. The α-diversity, species composition, and abundance of soil bacterial communities were analyzed using high-throughput sequencing. The effect of environmental factors on bacterial community structure was also examined. The results showed that the soil bacteria in the Huixian karst wetland can be divided into 49 phyla and 145 classes. The Shannon index of bacteria in the PF was significantly higher, and the Simpson index of bacteria in the NW is significantly lower, than in the other two land-use types. The dominant phyla (operational taxonomic units, OTUs>1%) in the NW were Proteobacteria (52.15%), Actinobacteria (15.16%), and Acidobacteria (8.80%); the dominant phyla in the PF were Proteobacteria (45.79%), Acidobacteria (17.20%), and Chloroflexi (11.75%); the dominant phyla in the DL were Proteus (51.42%), Acidobacteria (15.51%), and Chloroflexi (7.43%). The dominant classes (OTUs>1%) in the NW were α-Proteobacteria (17.98%), ß-Proteobacteria (13.72%), and Actinobacteria (13.13%); the dominant classes in the PF were Acidobacteria (14.35%), ß-Proteobacteria (13.37%), and δ-Proteobacteria (12.02%); the dominant classes in the DL were α-Proteobacteria (19.44%), Formobacteria (13.30%), and Acidobacteria (13.03%). Among the dominant OTUs (>0.3%), the dominant genera of in the NW were Sphingomonas (OTU2, 59), Micromonospora (OTU5, 24 and 50487), Gemmatimonas (OTU1), and Tenotrophomonas (OTU8); the dominant genera in the PF were Lysobacter (OTU4 and 115) and Aquabacterium (OTU33); the dominant genera in the DL were Sphingomonas (OTU85, 157 and 2916), Rhodanobacter (OTU19 and 52), and Penlobacterium (OTU60). A heatmap showed that there were significant differences in soil bacterial community structure among the three land-use types. Redundancy analysis showed that pH, soil organic carbon (SOC), total nitrogen (TN), alkali-hydrolyzable nitrogen (AN), exchangeable Mg2+, exchangeable Ca2+, soluble organic carbon (DOC), and available phosphorus (AP) were the main factors that affected the bacterial community structure in the Huixian karst wetland. These results indicate that changes in land-use types have significantly shaped the structure of soil bacterial communities in this area.


Assuntos
Agricultura , Bactérias/classificação , Oryza , Microbiologia do Solo , Áreas Alagadas , Cálcio/análise , Carbono/análise , China , Nitrogênio/análise , Fósforo/análise
17.
Iran J Public Health ; 48(9): 1566-1576, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31700812

RESUMO

Background: We aimed to investigate the association between lung cancer and tea-drinking habits of different subgroup populations. Methods: Systematic search of the PubMed, Web of Science, China National Knowledge Infrastructure (CNKI) and Sinomed databases from database construction until January 2017 for English and Chinese language articles on association of lung cancer and tea drinking. Meta-analysis was used to calculate the combined odds ratio (OR) value and its 95% confidence interval (95% CI). The Newcastle-Ottawa scale was used to evaluate the quality of the studies and Q-test and I2 was used for heterogeneity testing. Results: Forty two papers were included, 30 case-control studies included 14578 lung cancer patients and 180574 controls, 12 cohort studies included 543825 subjects, of which the outcome was 5085 with lung cancer. Tea drinkers were found to have a decreased OR of lung cancer compared with non-tea drinkers (OR 0. 80, 95% CI: 0. 73, 0. 87). Consumption of green, black or unspecified tea has a protective effect compared with not drinking tea at all. Increased intake of green tea to 7. 5 g per day can further reduce the OR of lung cancer (OR 0. 69, 95% CI: 0. 48-0. 98). Tea consumption had a protective effect against lung cancer in non-smokers, Further analysis found that drinking of one or more cups of tea a day has a protective effect on smokers (OR 0. 79, 95% CI: 0. 64-0. 96). Conclusion: Tea drinking could be a protective factor in lung cancer.

18.
Medicine (Baltimore) ; 98(47): e17840, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31764780

RESUMO

BACKGROUND: Asthma is a common chronic disease with heavy burden. The number of asthma patients may continue to grow in the next 10 years. Existing conventional treatments have problems in which a small number of patients do not respond, often accompanied by side effects, or are too expensive. Probiotics, prebiotics, and synbiotics have been widely used in allergic and inflammatory diseases including asthma. However, their effectiveness and safety are still obscure and deserve further investigation. OBJECTIVE: To assess the effect and safety of probiotics, prebiotics, and synbiotics in treating asthma. METHODS: We will summarize and meta-analyze randomized controlled trials (RCTs) of probiotics, prebiotics, and synbiotics for the treatment of asthma. RCTs comparing probiotics, prebiotics, and synbiotics with blank control, placebo, or conventional therapies will be included. RCTs comparing probiotics, prebiotics, and synbiotics plus conventional therapies with conventional therapies alone will also be included. The following electronic databases will be searched: PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure Database, Chinese Biomedical Literature Database, VIP Chinese Science and Technology Periodical Database, and Wanfang Data. The methodological quality of RCTs will be assessed using the Cochrane risk assessment tool. All trials included will be analyzed according to the criteria of the Cochrane Handbook. Review Manager 5.3, R-3.5.1 software will be used for publication bias analysis. Grading of recommendations assessment, development, and evaluation pro GDT web solution will be used for evidence evaluation. RESULTS: This review will evaluate the effects of probiotics, prebiotics, and synbiotics on symptoms, lung function, asthma exacerbations, quality of life, and safety in patients with asthma. CONCLUSIONS: This review will provide clear evidence to assess the effectiveness and safety of probiotics, prebiotics, and synbiotics for asthma. OSF REGISTRATION NUMBER: DOI 10.17605/OSF.IO/V7DM9.


Assuntos
Asma/terapia , Prebióticos , Probióticos/uso terapêutico , Projetos de Pesquisa , Simbióticos , Revisão Sistemática como Assunto , Humanos
19.
Medicine (Baltimore) ; 98(47): e17841, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31764781

RESUMO

BACKGROUND: Low endometrial receptivity is associated with infertility in women through multiple and complex mechanisms. Existing treatments are not always effective. Symptomatic drugs such as estradiol valerate and/or aspirin do not completely solve the problem. Traditional Chinese herbs have been widely used in infertility and uterine disease including low endometrial receptivity. However, their effectiveness and safety are still obscure and deserve further investigation. OBJECTIVE: To assess the effect and safety of traditional Chinese herbs in treating low endometrial receptivity. METHODS: We will summarize and meta-analyze randomized controlled trials (RCTs) of traditional Chinese herbs for the treatment of low endometrial receptivity. RCTs comparing traditional Chinese herbs with blank control, placebo, or conventional therapies will be included. RCTs comparing traditional Chinese herbs plus conventional therapies with conventional therapies alone will also be included. The following electronic databases will be searched: PubMed, Cochrane Library, EMBASE, CNKI, CBM, VIP, and WANFANG DATA. The methodological quality of RCTs will be assessed using the Cochrane risk assessment tool. All trials included will be analyzed according to the criteria of the Cochrane Handbook. Review Manager 5.3, R-3.5.1 software will be used for publication bias analysis. GRADE pro GDT web solution will be used for evidence evaluation. RESULTS: This review will evaluate the effects of traditional Chinese herbs on estradiol, progesterone, thickness, volume, and perfusion index(PI) of the endometrium, pregnancy rate, and symptoms. CONCLUSIONS: This review will provide clear evidence to assess the effectiveness and safety of traditional Chinese herbs for low endometrial receptivity. OSF REGISTRATION NUMBER: DOI 10.17605/OSF.IO/M85VT.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Infertilidade Feminina/tratamento farmacológico , Metanálise como Assunto , Projetos de Pesquisa , Revisão Sistemática como Assunto , Endométrio/fisiopatologia , Feminino , Humanos , Infertilidade Feminina/etiologia , Fitoterapia , Gravidez , Ensaios Clínicos Controlados Aleatórios como Assunto
20.
Immunotherapy ; 11(17): 1481-1490, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31713453

RESUMO

Aim: We performed a meta-analysis to explore the efficacy of immunotherapy for patients with squamous non-small-cell lung cancer (NSCLC). Materials & methods: Randomized clinical trials comparing immunotherapy with chemotherapy for advanced NSCLC patients were included. Results: A total of 11 trials (3112 patients) were included. PD-1/PD-L1 inhibitors demonstrated significant superiority to chemotherapy in overall survival (OS) (hazard ratio [HR]: 0.74; p < 0.001) and progression-free survival (PFS) (HR: 0.66; p < 0.001) for squamous NSCLC. The OS and PFS benefits of PD-1/PD-L1 inhibitors for squamous NSCLC were similar in subgroup analyses of line settings, PD-L1 expression and different study methodologies. No advantage in OS was found in advanced squamous NSCLC patients treated with atezolizumab (HR: 0.87; p = 0.087). Conclusion: PD-1/PD-L1 inhibitors significantly improved OS and PFS in advanced squamous NSCLC patients when compared with chemotherapy.

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