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1.
J Hazard Mater ; 443(Pt B): 130325, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36372023

RESUMO

The elimination of anion is of great importance from radioactive nuclear waste containing 99TcO4- by rationally designing anion-scavenging materials with high density of charge and more accessible adsorption sites. Herein, a tailor-made cationic organic polymer with donor-acceptor (D-A) structure, namely TrDCPN, was successfully synthesized by rationally modifying the benzimidazole unit for efficient trapping the perrhenate (ReO4-) as a 99Tc surrogate. Systematic control of the skeleton affect enables the material to integrate a variety of features, surmounting the long-term challenge of 99TcO4-/ReO4- remediation under extreme conditions of high acid/base and high ionic strength. Furthermore, the TrDCPN shows excellent affinity toward ReO4- in the existence of large excess of competitive anions (SO42-, NO3- and PO43-etc.) as well as promising reusability for trapping ReO4-. The excellent stability and separation were derived from the introduction of large conjugated modules, triazine core and hydrophobic. More importantly, the synthetic cationic organic polymer with D-A feature was first proved that the introduction of halogen can effectively enhance the backbone charge, and increase the adsorption capacity by synergy of ion exchange, electrostatic interaction and δ hole-anion interaction. The adsorption capacity of TrDCPN can be up to 420.3 mg/g and reach equilibrium within 20 min. It is noteworthy that TrDCPN successfully immobilizes ReO4- from simulated Hanford waste with a high separation efficiency of 93 %, providing a new paradigm for material design to dispose of the problem of radioactive pollutants in the environment.

2.
Food Chem ; 399: 133799, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35998490

RESUMO

Flesh quality is evaluated according to nutritional value and sensory quality. Cinnamaldehyde (CIN) improves mammalian meat quality, but research relating this to aquaculture is scarce. In this study, five doses of CIN (0, 36, 72, 108, 144 mg/kg diet) were fed to grass carp (Ctenopharyngodon idella) for 60 days. The results show that CIN supplementation increased nutritional value by increasing crude protein content. CIN also improved the sensory quality by increasing the pH and collagen content, decreasing shear force, lactate, and cooking loss. These changes may be related to changes in muscle fiber growth by increasing myofiber diameter. The increased myofiber diameter induced by CIN is associated with TOR mRNA and protein levels, and down-regulated FOXO3a mRNA levels, which might be associated with PTP1B/IGF1/PI3K/AKTs-TOR/FOXO3a signaling. Based on muscle crude protein content, optimal CIN supplementation dosage was 88.01 mg/kg.


Assuntos
Carpas , Doenças dos Peixes , Acroleína/análogos & derivados , Ração Animal/análise , Animais , Carpas/genética , Carpas/metabolismo , Dieta , Suplementos Nutricionais , Doenças dos Peixes/genética , Proteínas de Peixes/metabolismo , Imunidade Inata , Mamíferos/genética , Fibras Musculares Esqueléticas/metabolismo , Proteínas Musculares/metabolismo , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , RNA Mensageiro/genética , Transdução de Sinais
3.
Int J Endocrinol ; 2022: 4149468, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36389125

RESUMO

Objective: To investigate the correlation of psoas muscle index (PMI) with fragility vertebral fracture. Methods: A total of 184 middle-aged and elderly women were included in the study. We measured the bilateral psoas muscle area on the picture archiving and communication system (PACS) from computed tomography images and calculated PMI. We observed lateral radiographs of the thoracolumbar spine and assessed vertebral fractures using the Genant semiquantitative method. The T-score, bone mineral density (BMD) of the lumbar (L)1-4, femoral neck, and trochanter were measured by dual-energyX-ray absorptiometry (DXA). The data was collected and then statistically analyzed. Results: The PMI of the nonosteoporosis group was higher than that of the osteoporosis group (P value = 0.006). Height in the nonosteoporosis group was higher than that in the osteoporosis group (P value = 0.013). Weight, body mass index (BMI), left psoas muscle area, BMD of the L1-4, femoral neck, femoral trochanter, and T-score in the nonosteoporosis group were higher than those in the osteoporosis group (P value <0.001). The right psoas muscle area in the nonosteoporosis group was higher than that in the osteoporosis group (P value = 0.008). The incidence of combined thoracolumbar fracture was significantly higher in the osteoporosis group than that in the nonosteoporosis group (P value <0.001). For nonosteoporosis subjects, the PMI of the vertebral fracture group was lower than that of the nonvertebral fracture group (P value = 0.034). Conclusions: A decrease in height, weight, BMI, bilateral psoas muscle area, and PMI is associated with osteoporosis. Combined thoracolumbar fractures are more common in osteoporosis. Sarcopenia may be an independent risk factor for nonosteoporotic vertebral fractures.

5.
Front Chem ; 10: 1037542, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386000

RESUMO

Chemical analysis of atmospheric aerosols by conventional analytical methods is usually required to perform complicated and time-consuming sample preparation processes. In recent decades, ambient ionization mass spectrometry (AI-MS) methods have been proven to be simple, rapid, and effective analytical tools for direct analysis of various complex samples. In this work, we applied porous paper filters for direct adsorptive sampling of tobacco smoke, and then the sampled paper filters were performed the emitters of the paper spray ionization (PSI) device. An auto-sampling device was made to control the generation and collection of tobacco smoke. Nicotine, the typical compound of tobacco smoke, was used to optimize the key conditions of auto-sampling. Moreover, different types of tobacco smoke were also compared with multivariate variable analysis, and the makers of tobacco smoke from different sources of tobacco smoke were investigated. By using this method, direct sampling and analysis of a single tobacco sample can be completed within minutes. Overall, our results show that PSI-MS is a powerful tool that integrates collection, extraction, ionization, and identification analytes in smoke.

6.
World J Gastrointest Surg ; 14(10): 1169-1178, 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36386398

RESUMO

BACKGROUND: Immunoglobulin G4-related disease (IgG4-RD) is an immune-mediated condition characterized by abundant IgG4 positive plasma cells and fibrosis in the affected tissues. It affects most parts of the body; however, there are not many reports on IgG4-RD involving the colon. CASE SUMMARY: A 50-year-old man complaining of intermittent fever for more than two years was referred to our hospital. Based on various investigations before surgery, we diagnosed him with chronic perforation of the sigmoid colon caused by inflammatory change or tumor. IgG blood tests before the operation suggested IgG4-RD, and postoperative pathology confirmed this prediction. CONCLUSION: We present a patient with IgG4-RD with colon involvement, which is an uncommon site. This report will expand the understanding of IgG4-RD in unknown tissues.

7.
Front Nutr ; 9: 1012030, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36386925

RESUMO

To investigate a polysaccharide from Zingiber striolatum favorably modulates gut microbiota in mice fed a high-fat diet. Z. striolatum was utilized to extract the crude polysaccharide CZSP, which was subsequently refined using DEAE-52 cellulose and Sephadex G-150 to yield the novel polysaccharide Zingiber strioatum pure polysaccharide-1 (ZSPP-1). ZSPP-1 was an acidic heteroglycan made up of galactose, mannose, glucose, xylose, arabinose, glucuronic acid, and galacturonic acid with an average molecular weight of 1.57 × 106 Da. The structure of ZSPP-1 was investigated by FT-IR, methylation and NMR analysis, and the results denoted that the linkage structure types include T-Manp-linked, ß-Xylp-(1,2)-linked, ß-Galp-(1,4)-linked, α-GlcpA-(1,6)-linked, ß-Arap-(1,4)-linked, α-Glcp-(1,3,4,6)-linked, α-Glcp-(1,2)-linked, and ß-T-Xylp-linked, in which ß-Galp-(1,4)-linked and α-GalpA-(1,4)-linked might be the main linkage. The results of the intervention experiments showed that ZSPP-1 changed the intestinal flora structure of the Firmicutes and Bacteroidetes in obese mice, and promoted the growth of beneficial bacteria such as Akkermansia, Lactobacillus, and Bacteroides in the intestine. It also restored the imbalanced flora structure due to high-fat diet to normal. It also restored the imbalanced flora structure due to high-fat diet to normal. Z. striolatum polysaccharides presented a considerable advantage in alleviating high-fat diet induced obesity, which indicates that it can be further exploited as a natural functional food resource.

8.
J Prosthet Dent ; 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36396489

RESUMO

STATEMENT OF PROBLEM: The influence of silver nanoparticles on adhesive properties of Single Bond Universal (3M ESPE) and the antibacterial activity of silver nanoparticles-modified adhesives against Streptococcus mutans is unclear. PURPOSE: The purpose of this in vitro study was to evaluate the effect of silver nanoparticles on the dentin bond strength of modified adhesives and the antibacterial activity against the cariogenic pathogen S. mutans. MATERIAL AND METHODS: Single Bond Universal adhesive was used as the control. Silver nanoparticles were added to adhesives at 0.05% and 0.1% (by weight) (experimental groups), and scanning electron microscopy was used to observe the uniformity of the modified adhesives. The Single Bond Universal adhesive and the modified adhesives were then used to prepare dentin-composite resin blocks. The microtensile bond strength and microleakage of the prepared dentin-composite resin blocks were determined with or without thermocycling. The colony-forming units (CFU) of S. mutans cultured with the adhesives were evaluated, and the microtensile bond strength and microleakage of each group were tested after treatment with S. mutans. The differences in the microtensile bond strength or CFU were analyzed by using the 2-way analysis of variance and independent sample t test. The differences in microleakage between the groups were evaluated by using the Mann-Whitney test (α=.05). RESULTS: Silver nanoparticle-modified adhesives exhibited uniform morphologies without agglomeration and exhibited a homogeneous adhesive layer in dentin-composite resin blocks. The microtensile bond strength and microleakage of the modified adhesives were similar to those of the control group, with or without thermocycling (P>.05). However, thermocycling reduced the bond strength significantly (P<.001). Self-etch adhesives incorporated with silver nanoparticles showed significant antibacterial activities after less than 6 months of aging treatment. The modified adhesives did not exhibit a decreased bond strength after S. mutans exposure (P>.05), while the control group exhibited a markedly decreased bond strength after S. mutans exposure (P<.05). CONCLUSIONS: Silver nanoparticle-modified adhesives showed excellent antibacterial activities against S. mutans and resisted the destruction of dentin bond strength caused by S. mutans while not compromising the bonding properties of Single Bond Universal self-etch adhesives.

10.
Iran J Public Health ; 51(5): 1040-1048, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-36407737

RESUMO

Background: To analyze the effect of continuous nursing model based on WeChat public health education on self-management level and treatment compliance of stroke patients. Methods: Overall, 98 stroke patients admitted to the Second Affiliated Hospital of Chongqing Medical University, ChongQing, 400000,China from Feb 2018 to Feb 2019 were enrolled as the research objects. They were randomly and equally divided into experimental group and control group. The control group received routine nursing management model while the experimental group received continuous nursing model based on WeChat public health education to compare the self-management level, treatment compliance, life quality and other indexes between the two groups. Results: The overall self-management level in the experimental group was significantly higher than that in the control group (P<0.05). The life quality scores in both groups after nursing intervention increased, and the scores in the experimental group after nursing intervention were significantly higher than those in the control group (P<0.001). The total treatment compliance rate in the experimental group was higher than that in the control group (P=0.001). After nursing intervention, the readmission rate and complication rate in the experimental group were significantly lower than those in the control group (P<0.05). Conclusion: The implementation of continuous nursing model based on WeChat public health education for stroke patients can significantly enhance the self-management level, improve the cognitive level on stroke related-health knowledge, improve treatment compliance of patients in the nursing process, promote their physical and mental health, improve the life quality and effectively reduce the complication rate and readmission rate.

11.
Artigo em Inglês | MEDLINE | ID: mdl-36408347

RESUMO

Objective: To observe the effectiveness and safety of the Colles fracture treated with the integrated retainer pad splint and to compare the clinical and radiological outcomes of the integrated retainer pad splint and the traditional bamboo curtain splint in the treatment of the Colles fracture. Methods: A total of 100 patients with Colles fractures were randomly divided into two groups: the treatment group was fixed with the integrated retainer pad splint (IS), and the control group was fixed with the traditional bamboo curtain splint (TS).The range of wrist motion was measured at follow-up examinations, and volar inclination, ulnar deviation, and radial height were measured on radiographs. Regular follow-up wrist imaging examinations and functional examinations were performed before reduction, after reduction, and at the 1st, 3rd, 5th, and 8th weeks. The two groups were compared in terms of convenience, fracture healing time, X-ray data of volar inclination, ulnar deviation, radial height, and wrist joint function. The relevant data were analyzed with SPSS 25.0 statistical software. Results: There were no notable differences in gender, age, and injured side between IS and TS groups. In terms of operation time, IS was better than the TS group (P < 0.05), and the operation time in the IS group was shorter. On the basis of X-ray data of volar inclination, ulnar deviation, and radial height measured on radiographs, the difference between the IS and TS groups was statistically significant (P < 0.05), which showed that the IS group was more stable in fracture fixation and had less reduction loss during the treatment process. At the 8th week of treatment, the wrist Gartland-Werley score of the two groups showed that the two fixation methods are equivalent in restoring wrist joint function (P > 0.05); however, in terms of the excellent and good rate of wrist joint function, the IS group scored 96% was higher than the TS group (80%). Conclusion: Compared with the traditional bamboo curtain splint, the integrated retainer pad splint is more convenient and stable, and it has less reduction loss during the treatment. Repair of the Colles fracture using the integrated retainer pad splint with external fixation results in nearly normal return of function, which is significantly better than using the traditional bamboo curtain splint.

12.
Nat Commun ; 13(1): 6903, 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36371443

RESUMO

The current tumour-node-metastasis (TNM) staging system alone cannot provide adequate information for prognosis and adjuvant chemotherapy benefits in patients with gastric cancer (GC). Pathomics, which is based on the development of digital pathology, is an emerging field that might improve clinical management. Herein, we propose a pathomics signature (PSGC) that is derived from multiple pathomics features of haematoxylin and eosin-stained slides. We find that the PSGC is an independent predictor of prognosis. A nomogram incorporating the PSGC and TNM staging system shows significantly improved accuracy in predicting the prognosis compared to the TNM staging system alone. Moreover, in stage II and III GC patients with a low PSGC (but not in those with a high PSGC), satisfactory chemotherapy benefits are observed. Therefore, the PSGC could serve as a prognostic predictor in patients with GC and might be a potential predictive indicator for decision-making regarding adjuvant chemotherapy.


Assuntos
Neoplasias Gástricas , Humanos , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/genética , Neoplasias Gástricas/tratamento farmacológico , Prognóstico , Estadiamento de Neoplasias , Quimioterapia Adjuvante , Nomogramas , Estudos Retrospectivos
13.
Clin Case Rep ; 10(11): e6513, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36381061

RESUMO

Wilson's disease is an autosomal recessive inherited disease with congenital copper metabolism disorder, characterized by decreased ceruloplasmin and increased urine copper, which can involve multiple organs. This case was complicated by iron overload, which is of great value in differentiating hereditary hemochromatism.

15.
Comput Struct Biotechnol J ; 20: 5750-5760, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36382193

RESUMO

Although aging is an increasingly severe healthy, economic, and social global problem, it is far from well-modeling aging due to the aging process's complexity. To promote the aging modeling, here we did the quantitative measurement based on aging blood transcriptome. Specifically, the aging blood transcriptome landscape was constructed through ensemble modeling in a cohort of 505 people, and 1138 age-related genes were identified. To assess the aging rate in the linear dimension of aging, we constructed a simplified linear aging clock, which distinguished fast-aging and slow-aging populations and showed the differences in the composition of immune cells. Meanwhile, the non-linear dimension of aging revealed the transcriptome fluctuations with a crest around the age of 40 and showed that this crest came earlier and was more vigorous in the fast-aging population. Moreover, the aging clock was applied to evaluate the rejuvenation effect of molecules in vitro, such as Nicotinamide Mononucleotide (NMN) and Metformin. In sum, this study developed a de novo aging clock to evaluate age-dependent precise medicine by revealing its fluctuation nature based on comprehensively mining the aging blood transcriptome, promoting the development of personal aging monitoring and anti-aging therapies.

16.
Proc Inst Mech Eng H ; : 9544119221135627, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36394294

RESUMO

Recently, many different types of artificial discs have been introduced to persevere the biomechanical behavior of the cervical spine. This study compares the biomechanical behavior of single- and double-level cervical disc arthroplasty, that is "Prestige LP and Mobi-C" on the index and adjacent segment. A three-dimension finite element model of C2-C7 was developed and validated. In single-level prostheses, the Prestige LP or Mobi-C was implanted in the segment C5-C6, while the double-level arthroplasty was integrated at both segments C4-C5 and C5-C6 in the FE model. The intact FE and prosthesis-modified models were constrained from the inferior endplate of the vertebra C7 and applied a compressive load of 73.6 N with a moment load of 1 Nm on the odontoid process of the vertebra C2 to produce flexion/extension, lateral bending, and axial rotation. The prosthesis-modified model's range of motion and intradiscal pressure were determined and compared to the intact model. Also examined the impact of the prostheses on the stress at the bone-implant interface. The range of motion of the implanted segments in both single- and double-levels arthroplasty was increased while that of the adjacent segment of implanted segments decreased. The intradiscal pressure in both levels of arthroplasty was greater than in the intact model. In conclusion, Mobi-C's cervical prostheses could better preserve the normal range of motion and maintain intradiscal pressure than the Prestige LP.

17.
J Med Virol ; : e28302, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36369778

RESUMO

Our aim was to conduct a large epidemiologic analysis of the distribution of human papilloma virus (HPV) genotypes associated with cervical neoplasias and cancers at a major Chinese gynecologic center. The pathologic database was searched for cervical histopathologic diagnoses with prior HPV genotyping from liquid cervical cytology specimens obtained ≤6 months before biopsy. HPV testing was performed by using the Tellgenplex HPV27 or YanengBio HPV23 genotyping assays. A total of 40 352 cases meeting study criteria were identified. High risk human papillomavirus (hrHPV) was detected in 94.1% of squamous cancers compared to in only 83.3% of cervical adenocarcinomas. The prevalence of multiple HPV infections was highest in cervical intraepithelial neoplasia 1 (CIN1) (33.8%) and decreased with increasing severity of squamous lesions. The distribution of HPV genotypes was similar between CIN1 and histopathologic-negative cases. HPV16 was one of the three most common hrHPV genotypes before all histopathologic abnormalities, ranging from 72.0% for cervical cancers, 38.7% for CIN2/3/AIS, 13.1% for CIN1, and 9.1% for biopsy-negative cases. HPV16 and HPV18 accounted for over 87.2% of detected hrHPV genotypes for all glandular intraepithelial neoplastic lesions and cancers, whereas squamous lesions did not show this pattern. 80.3% of cervical cancers were associated with genotypes covered by HPV16/18 vaccines and 89.6% with genotypes covered by 9-valent vaccination.

18.
FEMS Microbiol Lett ; 369(1)2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36370448

RESUMO

Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is a serious contagious disease. MTB-encoded small regulatory RNA (sRNA) MTS2823 was reported to be upregulated in the plasma of TB patients. Nevertheless, whether MTS2823 is implicated in MTB drug resistance is unclear. Human macrophage cell line THP-1 was infected with the drug-susceptible strain H37Rv or the multidrug-resistant (MDR) strain 8462. Colony-forming unit assay was implemented for evaluating intracellular growth of the MTB strains. Enzyme-linked immunosorbent assay was used for measurement of inflammatory cytokines. Real-time quantitative polymerase chain reaction was utilized to assess MTS2823 and recombinase A (recA) expression in strains 8462 and H37Rv. Nitric oxide (NO) production in the MDR strain-infected THP-1 cells was measured. In this study, MTS2823 was found to display a low level in the MDR strain. Overexpressing MTS2823 promoted intracellular growth of the MDR strain and inhibited inflammatory cytokine and NO production in infected THP-1 cells. RecA might be a target of MTS2823 in the MDR strain. Overall, MTB-encoded sRNA MTS2823 displays a low level and regulates the growth of the MDR strain in THP-1 cells by modulating recA.


Assuntos
Mycobacterium tuberculosis , Tuberculose Resistente a Múltiplos Medicamentos , Tuberculose , Humanos , Tuberculose Resistente a Múltiplos Medicamentos/genética , Tuberculose Resistente a Múltiplos Medicamentos/microbiologia , Antituberculosos/farmacologia , Macrófagos/microbiologia , Citocinas/genética
19.
Mikrochim Acta ; 189(12): 467, 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36422716

RESUMO

A selective method for the determination of myosmine (a tobacco alkaloid) was developed using molecularly imprinted polymers (MIPs) based on Fe3O4@SiO2 and PAMAM dendrimers. Fe3O4@SiO2 with excellent magnetism and rapid separation performance was chosen as carrier for the MIPs. Moreover, the MIPs modified with PAMAM dendrimers exhibited a regular structure and abundant functional groups, which improve the efficiency of imprinting sites. The myosmine concentration was measured by HPLC with PDA detector, and the UV detection wavelength was set to 200 nm. The linear range of this assay was 13.2-400 mg/L with a correlation coefficient of 0.999, and the detection limit was 4.0 mg/L (S/N = 3). The MIPs have been used for the determination of myosmine in cigarettes, and the recovery range was 84.2-93.5%, with RSD values in the range 0.41-3.1%. In conclusion, our MIPs combine advantages of simple preparation and remarkable selectivity, which gives them excellent application prospects for the sensitive determination of trace myosmine in tobacco products.

20.
Int Orthop ; 2022 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-36422704

RESUMO

PURPOSE: Both robots and navigation are effective strategies for optimizing screw placement, as compared to freehand placement. However, few studies have compared the accuracy and efficiency of these two techniques. Thus, the purpose of this study is to compare the accuracy and efficiency of robotic and navigation-assisted screw placement in the spinal vertebrae. METHODS: The 24 spine models were divided into a robot- and navigation-assisted groups according to the left and right sides of the pedicle. The C-arm transmits image data simultaneously to the robot and navigates using only one scan. After screw placement, the accuracy of the two techniques were compared using "angular deviation" and "Gertzbein and Robbins scale" in different segments (C1-7, T1-4, T5-8, T9-12, and L1-S1). In addition, operation times were compared between robot- and navigation-assisted groups. RESULTS: Robots and navigation systems can simultaneously assist in screw placement. The robot-assisted group had significantly less angular deviation than the navigation-assisted group from C1 to S1 (p < 0.001). At the C1-7 and T1-4 segments, the robot-assisted group had a higher rate of acceptable screws than the robot-assisted group. However, at the T5-8, T9-12, and L1-S1 segments, no significant difference was found in the incidence of acceptable screws between the two groups. Moreover, robot-assisted screw placement required less operative time than navigation (p < 0.05). CONCLUSION: The robot is more accurate and efficient than navigation in aiding screw placement. In addition, robots and navigation can be combined without increasing the number of fluoroscopic views.

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