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1.
J Geriatr Cardiol ; 19(4): 265-275, 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35572218

RESUMO

BACKGROUND: The Essen risk score improves stratification of patients with acute ischemic stroke by early stroke recurrence. Recent study showed it could also predict myocardial infarction (MI). This study aimed to compare the Essen score's ability to predict cerebrovascular events with compared cardiovascular events. METHODS: We included patients with acute ischaemic stroke or transient ischaemic attack within seven days from the Third China National Stroke Registry. One-year cumulative event rates of combined vascular events (a composite of MI, stroke recurrence or vascular death) and cardiac events (a composite of MI, heart failure or cardiac death) was estimated using the Kaplan-Meier method. The predictive value of the Essen score was assessed with C-statistics. In multivariate Cox regression analyses, we assessed whether Essen score, etiological subtype and imaging parameters were associated with outcomes. RESULTS: Of 13,012 patients were included, the cumulative one-year event rates were 10.03% for combined vascular events and 0.77% for cardiac events, respectively. Compared with those with an Essen score < 3, patients with an Essen score ≥ 3 were more likely to have a subsequent combined vascular event [hazard ratio (HR) = 1.39, 95% CI: 1.24-1.55] and cardiac events (HR = 2.30, 95% CI: 1.53-3.44). The score tended to be more predictive of the risk of MI (C-statistic = 0.63, 95% CI: 0.55-0.71) and cardiac events (C-statistic = 0.62, 95% CI: 0.56-0.67) than stroke recurrence (C-statistic = 0.55, 95% CI: 0.54-0.57) and combined vascular events (C-statistic = 0.56, 95% CI: 0.54-0.57). In multivariable analysis after adjusted Essen score, patients with multiple acute infarctions or single acute infarctions and large artery atherosclerosis subtype were independently associated with an increased risk of combined vascular events. While the cardioembolism subtype was associated with an increased risk of cardiac events. CONCLUSIONS: The Essen score is potentially more suitable for risk stratification of cardiovascular events than cerebrovascular events. Moreover, future predictive tools should take brain imaging findings and cause of stroke into consideration.

2.
Ultrason Imaging ; : 1617346221099435, 2022 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-35574925

RESUMO

Echocardiography plays an important role in the clinical diagnosis of cardiovascular diseases. Cardiac function assessment by echocardiography is a crucial process in daily cardiology. However, cardiac segmentation in echocardiography is a challenging task due to shadows and speckle noise. The traditional manual segmentation method is a time-consuming process and limited by inter-observer variability. In this paper, we present a fast and accurate echocardiographic automatic segmentation framework based on Convolutional neural networks (CNN). We propose FAUet, a segmentation method serially integrated U-Net with coordinate attention mechanism and domain feature loss from VGG19 pre-trained on the ImageNet dataset. The coordinate attention mechanism can capture long-range dependencies along one spatial direction and meanwhile preserve precise positional information along the other spatial direction. And the domain feature loss is more concerned with the topology of cardiac structures by exploiting their higher-level features. In this research, we use a two-dimensional echocardiogram (2DE) of 88 patients from two devices, Philips Epiq 7C and Mindray Resona 7T, to segment the left ventricle (LV), interventricular septal (IVS), and posterior left ventricular wall (PLVW). We also draw the gradient weighted class activation mapping (Grad-CAM) to improve the interpretability of the segmentation results. Compared with the traditional U-Net, the proposed segmentation method shows better performance. The mean Dice Score Coefficient (Dice) of LV, IVS, and PLVW of FAUet can achieve 0.932, 0.848, and 0.868, and the average Dice of the three objects can achieve 0.883. Statistical analysis showed that there is no significant difference between the segmentation results of the two devices. The proposed method can realize fast and accurate segmentation of 2DE with a low time cost. Combining coordinate attention module and feature loss with the original U-Net framework can significantly increase the performance of the algorithm.

3.
Oxid Med Cell Longev ; 2022: 3335887, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35528523

RESUMO

In ischemic stroke (IS), accumulation of the misfolded proteins in the endoplasmic reticulum (ER) and mitochondria-induced oxidative stress (OS) has been identified as the indispensable inducers of secondary brain injury. With the increasing recognition of an association between ER stress and OS with ischemic stroke and with the improved understanding of the underlying molecular mechanism, novel targets for drug therapy and new strategies for therapeutic interventions are surfacing. This review discusses the molecular mechanism underlying ER stress and OS response as both causes and consequences of ischemic stroke. We also summarize the latest advances in understanding the importance of ER stress and OS in the pathogenesis of ischemic stroke and discuss potential strategies and clinical trials explicitly aiming to restore mitochondria and ER dynamics after IS.


Assuntos
AVC Isquêmico , Retículo Endoplasmático/metabolismo , Estresse do Retículo Endoplasmático/fisiologia , Humanos , Mitocôndrias/metabolismo , Transdução de Sinais/fisiologia
4.
Chem Asian J ; 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35535732

RESUMO

Electrochemical reduction of CO 2 to produce valuable multi-carbon products is a promising avenue for promoting CO 2 conversion and achieving renewable energy storage, and it has also attracted considerable attention recently. However, the synthesis of Cu electrode with a controllable electrochemical active surface area (ECSA) to understand its role in CO 2 reduction to C 2 H 4 remains challenging. Herein, a series of Cu electrodes with different ECSA is synthesized through a simple oxidation-reduction approach. We reveal that the improved selectivity of C 2 H 4 is proportional to the ECSA of Cu in the low ECSA range, and a further increase in ECSA has a negligible effect on its selectivity. The enlarged surface area could strengthen the local pH effect near the surface of Cu electrode and suppress the generation of C 1 products as well as H 2 . The study provides a feasible strategy to rationally design electrocatalysts with high electrochemical CO 2 reduction performances.

5.
J Neurol ; 2022 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-35511281

RESUMO

BACKGROUND: Patients with ischemic stroke and diabetes are classified as extreme risk for secondary prevention, with much attention and specific management. However, the up-to-date information regarding the burden of diabetes in acute ischemic stroke (AIS) patients is lacking in China, and evidence for an association between diabetes and in-hospital outcomes after AIS remains controversial. METHODS: This quality improvement study was conducted at 1,476 participating hospitals in the Chinese Stroke Center Alliance between 2015 and 2019. Prevalence of diabetes was evaluated in the overall study population and different subgroups. The association between diabetes and in-hospital outcomes in AIS patients was analyzed by using multivariable logistic regression analysis and propensity score-matched analysis. RESULTS: Of 838,229 patients with AIS, 286,252 (34.2%) had diabetes/possible diabetes. The prevalence of diabetes/possible diabetes was higher in women than in men (37.6% versus 32.1%). Patients with diabetes/possible diabetes had higher rates of adverse in-hospital outcomes than those without. Multivariable analysis revealed a significant association between diabetes/possible diabetes and adverse in-hospital outcomes (all-cause mortality: odds ratio [OR], 1.30 [95% confidence interval [CI], 1.23-1.38]; major adverse cardiovascular events (MACEs): OR, 1.08 [95% CI, 1.06-1.10]) in AIS patients. The excess risk of in-hospital outcomes still remained in AIS patients with diabetes/possible diabetes after propensity score-matching analysis (all-cause mortality: OR, 1.26 [95% CI, 1.17-1.35]; MACEs: OR, 1.07 [95% CI, 1.05-1.10]). CONCLUSION: Diabetes was highly prevalent among AIS patients in China and associated with worse in-hospital outcomes. Greater efforts to increase targeted approach to secondary prevention treatments of diabetes in AIS patients are warranted.

6.
Aging (Albany NY) ; 14(undefined)2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35537818

RESUMO

BACKGROUND: We previously identified a novel lncRNA, CRART16, that could induce cetuximab resistance in colorectal cancer cells. This study explored the relationship of CRART16 expression to gastric cancer progression and the molecular mechanisms involved. METHODS: We evaluated CRART16 expression in gastric cancer tissues and adjacent normal tissues from the TCGA database and our hospital. Besides, we assessed its relationship with the overall survival (OS) of patients with gastric cancer. The effects of CRART16 on gastric cancer angiogenesis were determined by endothelial tube formation assay, spheroid sprouting assay, HUVEC invasion assay, and chick embryo chorioallantoic membrane (CAM) assay. The involvement of the lncRNA CRART16/miR-122-5p/FOS axis was analyzed by western blotting and dual-luciferase reporter assay. The functions of CRART16 were confirmed in xenograft mouse models. RESULTS: We found that CRART16 was substantially overexpressed in gastric cancer tissues compared with normal tissues, based on the TCGA database and our clinical samples. High expression of CRART16 correlated with more advanced tumor stages and poor prognosis. Overexpression of CRART16 in gastric cancer cells promoted proliferation, colony formation, angiogenesis, and bevacizumab resistance in vitro, and it promoted tumor growth and angiogenesis in vivo, and vice versa. CRART16 was found to downregulate miR-122-5p by acting as a sponge, upregulating the target oncogene FOS. Afterward, the increased FOS expression led to the upregulation of VEGFD. CONCLUSION: Our findings demonstrate that CRART16 promotes angiogenesis in vitro and in vivo, and CRART16 is a prognostic marker and therapeutic target in gastric cancer.

7.
J Transl Med ; 20(1): 193, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35509104

RESUMO

PURPOSE: We develop a new risk score to predict patients with stroke-associated pneumonia (SAP) who have an acute intracranial hemorrhage (ICH). METHOD: We applied logistic regression to develop a new risk score called ICH-LR2S2. It was derived from examining a dataset of 70,540 ICH patients between 2015 and 2018 from the Chinese Stroke Center Alliance (CSCA). During the training of ICH-LR2S2, patients were randomly divided into two groups - 80% for the training set and 20% for model validation. A prospective test set was developed using 12,523 patients recruited in 2019. To further verify its effectiveness, we tested ICH-LR2S2 on an external dataset of 24,860 patients from the China National Stroke Registration Management System II (CNSR II). The performance of ICH-LR2S2 was measured by the area under the receiver operating characteristic curve (AUROC). RESULTS: The incidence of SAP in the dataset was 25.52%. A 24-point ICH-LR2S2 was developed from independent predictors, including age, modified Rankin Scale, fasting blood glucose, National Institutes of Health Stroke Scale admission score, Glasgow Coma Scale score, C-reactive protein, dysphagia, Chronic Obstructive Pulmonary Disease, and current smoking. The results showed that ICH-LR2S2 achieved an AUC = 0.749 [95% CI 0.739-0.759], which outperforms the best baseline ICH-APS (AUC = 0.704) [95% CI 0.694-0.714]. Compared with the previous ICH risk scores, ICH-LR2S2 incorporates fasting blood glucose and C-reactive protein, improving its discriminative ability. Machine learning methods such as XGboost (AUC = 0.772) [95% CI 0.762-0.782] can further improve our prediction performance. It also performed well when further validated by the external independent cohort of patients (n = 24,860), ICH-LR2S2 AUC = 0.784 [95% CI 0.774-0.794]. CONCLUSION: ICH-LR2S2 accurately distinguishes SAP patients based on easily available clinical features. It can help identify high-risk patients in the early stages of diseases.


Assuntos
Pneumonia , Acidente Vascular Cerebral , Glicemia , Proteína C-Reativa , Hemorragia Cerebral/complicações , Humanos , Hemorragias Intracranianas/complicações , Pneumonia/complicações , Prognóstico , Estudos Prospectivos , Fatores de Risco , Acidente Vascular Cerebral/complicações
8.
Sci Total Environ ; : 155695, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35525347

RESUMO

In the Southern Ocean, the living environment of organisms has changed due to the dramatic increase in melting sea ice and the loss of glaciers, which have consequently caused substantial changes in biodiversity. Samples of pelagic ciliates from 13 sites were collected as bioindicators to demonstrate the relationship between spatial distribution patterns and environmental heterogeneity affected by sea ice melting and to reveal the community assembly mechanisms in the Ross Sea. Univariate analyses and multivariate analyses were effective tools demonstrating clear spatial patterns and providing a sufficient explanation to interpret strong correlations between pelagic ciliate communities and environmental variations, especially the distribution pattern of nutrients and Chl a. Moreover, environmental heterogeneity might affect the co-occurrence network complexity of ciliate communities. Furthermore, our results also indicated that stochastic processes play a significant role in the community assembly of pelagic ciliates. This study examined the controlling mechanisms of environmental heterogeneity affected by sea ice melting on pelagic ciliate communities and provided explanations for the community assembly of pelagic ciliates in polar marine ecosystems.

9.
EBioMedicine ; 80: 104054, 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35576642

RESUMO

BACKGROUND: Post-stroke inflammation biomarker high-sensitivity C-reactive protein (hsCRP) increases cerebral infarct size and results in functional disability directly, it also contributes to the formation and maturation of atherosclerotic plaques, which increase the risk of stroke recurrence and results in functional disability indirectly. However, no study has quantified how much functional disability was mediated by stroke recurrence. METHODS: Patients with acute ischaemic stroke within 7 days and admitted to 169 hospitals in the Third China National Stroke Registry were analyzed. Blood samples were collected within 24 h of admission. Stroke recurrence and functional disability (defined as a modified Rankin scale score ≥ 2) were assessed via face-to-face interviews at three months. Mediation analysis under the counterfactual framework was performed to examine the potential causal chain in which stroke recurrence may mediate the relationship between hsCRP and functional outcome. Sensitivity analyses were performed across different subgroups and on different scales of hsCRP measurement. FINDINGS: Of the 7603 analyzed patients (mean [SD] age, 62.3 [11.3] years; 2392 [31.5%] women), the median (interquartile range [IQR]) of NIHSS score was 3.0 (1.0-6.0). The median (IQR) level of hsCRP was 1.73 (0.81-4.38) mg/L. A total of 496 (6.5%) cases of stroke recurrence and 1884 (24.8%) cases of functional disability were observed at the 90-day follow-up. Each SD increase in the concentration of hsCRP was associated with an increased risk of stroke recurrence (adjusted odds ratio [aOR], 1.11; 95% CI, 1.04-1.18) and disability (aOR, 1.14; 95% CI, 1.08-1.20) within 90 days. Of 1884 functionally disabled patients, only 16.0 % (n = 302) of patients experienced stroke recurrence before functional disability. Stroke recurrence during follow-up explained 16.52% (95% CI, 5.79%-27.25%) of the relationship between hsCRP and functional disability. Sensitivity analyses in different subgroups and on different scales of hsCRP measurement showed comparable results. INTERPRETATION: Stroke recurrence mediates less than 20% of the association between hsCRP and functional disability at 90 days among patients with acute ischaemic stroke. In addition to typical secondary prevention strategies for preventing stroke recurrence, more attention should be paid to novel anti-inflammatory therapy to improve functional outcomes. FUNDING: Beijing Natural Science Foundation, the National Key R&D Program of China, the National Natural Science Foundation of China, and the Beijing Municipal Science & Technology Commission.

10.
Biosens Bioelectron ; 211: 114349, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35576722

RESUMO

DNA nanomachines, a delicate type of molecular machines, have been a research hotspot in biotechnology and materials. Here a two-dimensional (2D) DNA walking nanomachine with high working efficiency and low cost was easily assembled by using graphene oxide (GO) as the working platform for precisely fluorescent bioassay through the binding of target hepatitis B virus DNA (HBV-DNA) and the driving force of Exonuclease III (Exo III). The presence of HBV-DNA made continuous Exo III digestion of the FAM-modified DNA (FAM-DNA) in double-strand DNA (dsDNA) part in a burnt-bridge mechanism to output a "one-to-more" amplified signal. Accordingly, a 2D DNA walking nanomachine with simple operation and high cost-performance ratio was constructed. The walking speed of nanomachine was found to be regulated by loading DNA density on single sheet of GO. Furthermore, this nanomachine had low background since the dual energy transfer including fluorescence resonance energy transfer (FRET) from FAM to BHQ1 and the long-range resonance energy transfer (LrRET) from FAM to GO, making the biosensing applications highly promising.

11.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 44(2): 286-289, 2022 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-35538764

RESUMO

Objective To evaluate the safety and effectiveness of laparoscopic common bile duct exploration in the treatment of common bile duct stones. Methods A retrospective analysis was conducted for 158 patients with cholecystolithiasis and choledocholithiasis admitted to the Number One Hospital of Zhangjiakou from January 2015 to December 2019.The patients were assigned into three groups according to the diameters of cystic duct and common bile duct,degrees of abdominal infection and tissue edema,and operation method.Group A(16 cases):laparoscopic cholecystectomy,transcystic choledochoscopic exploration for stone removal;Group B(94 cases):laparoscopic cholecystectomy,common bile duct incision exploration combined with choledochoscopy for stone removal,T tube drainage;Group C(48 cases):laparoscopic cholecystectomy,common bile duct incision exploration combined with choledochoscopy for stone removal,primary closure of the common bile duct.The operation time,residual rate of stones,and complication(bleeding,bile leakage,and wound infection) rate were compared between groups. Results The operation time of groups A,B,and C was(95.1±14.7),(102.2±18.1),(110.1±16.4) minutes,respectively,which showed no statistical difference between each other(F=0.020,P=0.887).One case in group A had residual stones,while no residual stone appeared in groups B and C.The overall stone clearance rate was 99.4% and the overall complication rate was 1.9%.There was no perioperative death. Conclusion It is generally safe and effective to carry out laparoscopic cholecystectomy and common bile duct exploration for stone removal in suitable populations.


Assuntos
Coledocolitíase , Cálculos Biliares , Laparoscopia , Coledocolitíase/cirurgia , Ducto Colédoco/cirurgia , Cálculos Biliares/cirurgia , Humanos , Laparoscopia/métodos , Estudos Retrospectivos
12.
Cancer Lett ; 538: 215693, 2022 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-35472437

RESUMO

Owing to the lack of early diagnosis, pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal tumours. Because acinar-to-ductal metaplasia (ADM) is a critical process to pancreatic regeneration and PDAC initiation, we applied GSE65146, a dataset composed of transcripts at different time points in wild-type and KrasG12D mutant mice upon pancreatitis induction, to obtain regeneration- and tumour initiation-related genes. By overlapping with genes differentially expressed in human PDAC, we defined the initiation- and progression-related genes, and the most prognostic gene, SULF2, was selected for further verification. By using multiple PDAC genetically engineered murine models (GEMMs), we further verified that the expression of SULF2 was increased at the ADM and PDAC stages. Functionally, SULF2 was able to promote the dedifferentiation of acinar cells as well as the metastatic ability of PDAC. Additionally, our study revealed that SULF2 could enhance TGFß-SMAD signalling via GDF15. More importantly, serum SULF2 was elevated in patients with PDAC, and in combination with CA19-9, it provided a better method for PDAC diagnosis. Herein, our study screened out key genes for the initiation and progression of PDAC, providing potential indicators for the diagnosis of the disease.

13.
BMC Cancer ; 22(1): 429, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35443644

RESUMO

BACKGROUND: The relationship between pyroptosis and cancer is complex. It is controversial that whether pyroptosis represses or promotes tumor development. This study aimed to explore prognostic molecular characteristics to predict the prognosis of breast cancer (BRCA) based on a comprehensive analysis of pyroptosis-related gene expression data. METHODS: RNA-sequcing data of BRCA were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Ominibus (GEO) datasets. First, pyroptosis-related differentially expressed genes (DEGs) between normal and tumor tissues were identified from the TCGA database. Based on the DEGs, 1053 BRCA patients were divided into two clusters. Second, DEGs between the two clusters were used to construct a signature by a least absolute shrinkage and selection operator (LASSO) Cox regression model, and the GEO cohort was used to validate the signature. Various statistical methods were applied to assess this gene signature. Finally, Single-sample gene set enrichment analysis (ssGSEA) was employed to compare the enrichment scores of 16 types of immune cells and 13 immune-related pathways between the low- and high-risk groups. We calculated the tumor mutational burden (TMB) of TCGA cohort and evaluated the correlations between the TMB and riskscores of the TCGA cohort. We also compared the TMB between the low- and high-risk groups. RESULTS: A total of 39 pyroptosis-related DEGs were identified from the TCGA-breast cancer dataset. A prognostic signature comprising 16 genes in the two clusters of DEGs was developed to divide patients into high-risk and low-risk groups, and its prognostic performance was excellent in two independent patient cohorts. The high-risk group generally had lower levels of immune cell infiltration and lower activity of immune pathway activity than did the low-risk group, and different risk groups revealed different proportions of immune subtypes. The TMB is higher in high-risk group compared with low-risk group. OS of low-TMB group is better than that of high-TMB group. CONCLUSION: A 16-gene signature comprising pyroptosis-related genes was constructed to assess the prognosis of breast cancer patients and its prognostic performance was excellent in two independent patient cohorts. The signature was found closely associated with the tumor immune microenvironment and the potential correlation could provide some clues for further studies. The signature was also correlated with TMB and the mechanisms are still warranted.


Assuntos
Neoplasias da Mama , Biomarcadores Tumorais/genética , Neoplasias da Mama/genética , Feminino , Humanos , Prognóstico , Piroptose/genética , Microambiente Tumoral/genética
14.
Artigo em Inglês | MEDLINE | ID: mdl-35449325

RESUMO

After years of governance, China's PM2.5 pollution has improved significantly, but some problems remain. PM2.5 is the carrier of many heavy metals, and it has a stronger capacity to carry heavy metals, causing more significant harm to public health. At present, most of the studies on PM2.5-bound heavy metals in China are based on individual cities, and there are few studies from China's provincial capital cities. Given the above reasons, this paper described the pollution characteristics of PM2.5-bound heavy metal (Hg, Pb, Cd, As, Cr(VI), Ni) in 29 provincial capital cities in China (except Hohhot and Lhasa). Furthermore, the human health risk assessment recommended by US EPA was used to evaluate the carcinogenic and non-carcinogenic risks of the male adults, the female adults, and the children. The results indicated that (1) the children were facing a higher risk value of PM2.5-bound heavy metals than the adults; (2) Cr, As, and Pb were the top three elements that contributed to the average non-carcinogenic risk, while Cr(VI), As, and Cd were the three elements with the highest carcinogenic risk; and (3) the concentrations of PM2.5-bound heavy metals in the provincial capital cities of northern China were worse than those in the South. In response to the above conclusions, this paper put forward: (1) the government should strengthen the management of metallurgical industry and coal-burning industrial enterprises; (2) establish a real-time monitoring system for toxic and harmful heavy metal pollutants carried in the atmosphere as soon as possible; (3) concern on the regional synergies to form pollution control metropolitan areas.

15.
Glob Chang Biol ; 2022 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-35366335

RESUMO

Temperature sensitivity (Q10 ) of soil organic matter (SOM) decomposition is an important parameter in models of the global carbon (C) cycle. Previous studies have suggested that substrate quality controls the intrinsic Q10 , whereas environmental factors can impose large constraints. For example, physical protection of SOM and its association with minerals attenuate the apparent Q10 through reducing substrate availability and accessibility ([S]). The magnitude of this dampening effect, however, has never been quantified. We simulated theoretical Q10 changes across a wide range of [S] and found that the relationship between Q10 and the log10 -transformed [S] followed a logistic rather than a linear function. Based on the unique Holocene paleosol chronosequence (7 soils from ca. 500 to 6900 years old), we demonstrated that the Q10 decreased nonlinearly with soil age up to 1150 years, beyond which Q10 remained stable. Hierarchical partitioning analysis indicated that an integrated C availability index, derived from principal component analysis of DOC content and parameters reflecting physical protection and mineral association, was the main explanatory variable for the nonlinear decrease of Q10 with soil age. Microbial inoculation and 13 C-labelled glucose addition showed that low C availability induced by physical protection and minerals association attenuated Q10 along the chronosequence. A separate soil incubation experiment indicated that Q10 increased exponentially with activation energy (Ea ) in the modern soil, suggesting that SOM chemical complexity regulates Q10 only when C availability is high. In conclusion, organic matter availability strongly decreased with soil age, whereas Michelis-Menten kinetics defines the Q10 response depending on C availability, but Arrhenius equation describes the effects of increasing substrate complexity.

16.
Chembiochem ; : e202200038, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35442561

RESUMO

Protopanaxadiol (PPD), a main ginseng metabolite, exerts powerful anticancer effects against multiple types of cancer; however, its cellular targets remain elusive. Here, we synthesized a cell-permeable PPD probe via introducing a bifunctional alkyne-containing diazirine photo-crosslinker and performed a photoaffinity labeling-based chemoproteomic study. We identified retinoblastoma binding protein 4 (RBBP4), a chromatin remodeling factor, as an essential cellular target of PPD in HCT116 colorectal cancer cells. PPD significantly decreased RBBP4-dependent trimethylation at lysine 27 of histone H3 (H3K27me3), a crucial epigenetic marker that correlates with histologic signs of colorectal cancer aggressiveness, and PPD inhibition of proliferation and migration of HCT116 cells was antagonized by RBBP4 RNA silencing. Collectively, our study highlights a previously undisclosed anti-colorectal cancer cellular target of the ginseng metabolite and advances the fundamental understanding of RBBP4 functions via a chemical biology strategy.

17.
China CDC Wkly ; 4(13): 265-270, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35433089

RESUMO

What is already known about this topic?: The prevalence of cardiovascular disease (CVD) in China is high, while effective prevention and proper management is lacking. No available indicators were found before 2016 that could comprehensively evaluate different aspects of CVD prevention and treatment. What is added by this report?: Constructed by combining data from multiple dimensions, China cardiovascular health index (CHI) has provided a practical indicator for each provincial-level administrative division (PLAD) to comprehensively understand its overall level and rankings of the specific dimensions of cardiovascular health. What are the implications for public health practice?: The CHI will be beneficial for each PLAD to identify weak aspects in CVD control and prevention and redistribute resources to the most needed areas.

18.
Adv Sci (Weinh) ; : e2104780, 2022 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-35474450

RESUMO

Carbonaceous materials are considered strong candidates as anode materials for sodium-ion batteries (SIBs), which are expected to play an indispensable role in the carbon-neutral era. Herein, novel braided porous carbon fibres (BPCFs) are prepared using the chemical vapour deposition (CVD) method. The BPCFs possess interwoven porous structures and abundant vacancies. The growth mechanism of the BPCFs can be attributed to the polycrystalline transformation of the nanoporous copper catalyst in the early stage of CVD process. Density functional theory calculations suggest that the Na+ adsorption energies of the mono-vacancy edges of the BPCFs (-1.22 and -1.09 eV) are lower than that of an ideal graphene layer (-0.68 eV), clarifying in detail the adsorption-dominated sodium storage mechanism. Hence, the BPCFs as an anode material present an outstanding discharge capacity of 401 mAh g-1 at 0.1 A g-1 after 500 cycles. Remarkably, this BPCFs anode, under high-mass-loading of 5 mg cm-2, shows excellent long-term cycling ability with a reversible capacity of 201 mAh g-1 at 10 A g-1 over 1000 cycles. This study provided a novel strategy for the development of high-performance carbonaceous materials for SIBs.

19.
Purinergic Signal ; 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35478452

RESUMO

Upregulation of P2X3 receptor (P2X3R) has been strongly implicated in nociceptive signaling including bone cancer pain (BCP). The present study, using rat bone cancer model, aimed to explore the role of P2X3R in regulating rat pain behavior under the intervention of electroacupuncture (EA). The BCP model was successfully established by injection with MRMT-1 breast cancer cell into the medullary cavity of left tibia for 3 × 104 cells/3 µL PBS in rats as revealed by obvious bone destruction, decreased paw withdrawal thresholds (PWTs), and reduced paw withdrawal latencies (PWLs). Western blot analyses showed that P2X3R expression was significantly upregulated in ipsilateral lumbar 4-6 (L4-6) dorsal root ganglia (DRG), but the difference not seen in spinal cord dorsal horn (SCDH). With the in-depth study of P2X3R activation, we observed that intrathecal injection of P2X3R agonist α,ß-meATP aggravated MRMT-1 induced BCP, while injection of P2X3R inhibitor A-317491 alleviated pain. Subsequently, we demonstrated that BCP induced mechanical allodynia and thermal hyperalgesia were attenuated after EA treatment. Under EA treatment, total P2X3R protein expression in ipsilateral DRGs was decreased, and it is worth mentioning that decreased expression of P2X3R membrane protein, which indicated that both the expression and membrane trafficking of P2X3R were inhibited by EA. The immunofluorescence assay showed that EA stimulation exerted functions by reducing the expression of P2X3R-positive cells in ipsilateral DRGs of BCP rats. Ca2+ imaging analysis revealed that the EA stimulation decreased the percentage of α,ß-meATP responsive neurons in DRGs and inhibited calcium influx. Notably, the inhibitory effect of EA on mechanical allodynia and nociceptive flinches was abolished by intrathecal injection of α,ß-meATP. These findings demonstrated EA stimulation ameliorated mechanical allodynia and thermal hyperalgesia in rat model of MRMT-1-induced BCP. EA exerts analgesic effect on BCP by reducing the overexpression and functional activity of P2X3R in ipsilateral DRGs of BCP rats. Our work first demonstrates the critical and overall role of P2X3R in EA's analgesia against peripheral sensitization of MRMT-1-induced BCP and further supports EA as a potential therapeutic option for cancer pain in clinic.

20.
J Med Virol ; 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35415847

RESUMO

The identification of circulating proteins associated with acquired immunodeficiency syndrome-related non-Hodgkin lymphoma (AIDS-NHL) may help in the development of promising biomarkers for screening, diagnosis, treatment, and prognosis. Here, we used quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins (DEPs) in plasma collected from patients with AIDS-NHL and human immunodeficiency virus (HIV)-infected patients without NHL (HIV+ ). Proteins with a log2 (fold change) in abundance >0.26 and p < 0.05 were considered differentially abundant. In total, 84 DEPs were identified, among which 20 were further validated as potential biomarkers, with immunoglobulin and complement components being the most common proteins. Some of the proteins were further verified in a retrospective analysis of the medical records of patients in a larger cohort. These markedly altered proteins were found to mediate pathophysiological pathways that likely contribute to AIDS-NHL pathogenesis, such as the humoral immune response, complement activation, and complement and coagulation cascades. Our findings provide a new molecular understanding of AIDS-NHL pathogenesis and provide new evidence supporting the identification of these proteins as possible biomarkers in AIDS-NHL.

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