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1.
J Emerg Manag ; 20(2): 143-155, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35451050

RESUMO

In response to several mass-casualty shootings in the United States, multiple legislative bodies have promoted or adopted laws that restrict the magazine capacity of firearms. The stated intent of these laws is to reduce the number of rounds a bad actor can fire in a mass-casualty situation. However, objectors argue that the laws not only are ineffective but could also cause an undue burden on the ability of law-abiding gun owners to protect themselves. Using agent-based simulation modeling, a home-invasion model including two-armed bad actors and a one-armed homeowner was created to test this hypothesis. The homeowner engaged the bad actors with different capacity magazines, and the survival rate was recorded and compared across the spectrum of capacities. This model showed that reducing the homeowner's magazine capacity resulted in an increased casualty rate for the homeowner.


Assuntos
Armas de Fogo , Incidentes com Feridos em Massa , Ferimentos por Arma de Fogo , Humanos , Estados Unidos
2.
J Clin Sleep Med ; 18(1): 193-202, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34310277

RESUMO

STUDY OBJECTIVES: We evaluated the interrater reliabilities of manual polysomnography sleep stage scoring. We included all studies that employed Rechtschaffen and Kales rules or American Academy of Sleep Medicine standards. We sought the overall degree of agreement and those for each stage. METHODS: The keywords were "Polysomnography (PSG)," "sleep staging," "Rechtschaffen and Kales (R&K)," "American Academy of Sleep Medicine (AASM)," "interrater (interscorer) reliability," and "Cohen's kappa." We searched PubMed, OVID Medline, EMBASE, the Cochrane library, KoreaMed, KISS, and the MedRIC. The exclusion criteria included automatic scoring and pediatric patients. We collected data on scorer histories, scoring rules, numbers of epochs scored, and the underlying diseases of the patients. RESULTS: A total of 101 publications were retrieved; 11 satisfied the selection criteria. The Cohen's kappa for manual, overall sleep scoring was 0.76, indicating substantial agreement (95% confidence interval, 0.71-0.81; P < .001). By sleep stage, the figures were 0.70, 0.24, 0.57, 0.57, and 0.69 for the W, N1, N2, N3, and R stages, respectively. The interrater reliabilities for stage N2 and N3 sleep were moderate, and that for stage N1 sleep was only fair. CONCLUSIONS: We conducted a meta-analysis to generalize the variation in manual scoring of polysomnography and provide reference data for automatic sleep stage scoring systems. The reliability of manual scorers of polysomnography sleep stages was substantial. However, for certain stages, the results were poor; validity requires improvement. CITATION: Lee YJ, Lee JY, Cho JH, Choi JH. Interrater reliability of sleep stage scoring: a meta-analysis. J Clin Sleep Med. 2022;18(1):193-202.


Assuntos
Fases do Sono , Sono , Criança , Humanos , Variações Dependentes do Observador , Polissonografia , Reprodutibilidade dos Testes
3.
Bioresour Technol ; 346: 126605, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34953994

RESUMO

Gas fermentation utilizes syngas converted from biomass or waste as feedstock. A bubble column reactor for pressurizing was designed to increase the mass transfer rate between gas and liquid, and reduce energy consumption by medium agitation. Thermococcus onnurineus, a hydrogenic CO-oxidizer, was cultured initially under ambient pressure with the initial inlet gas composition; 60% CO and 40% N2. The maximum H2 productivity was 363 mmol/l/h, without pH adjustment. When additional pressure was applied, the pH rapidly declined; this may be attributed to the increased CO2 solubility under pressure. By controlling pH, H2 productivity increased up to 450 mmol/l/h; which is comparable to the previously reported H2 productivity in a continuous stirred tank reactor. The results may suggest energy saving potentials of bubble column reactors in gas fermentation. This finding may be applied to other gas fermentation processes, as syngas itself contains CO2 and many microbial processes also release CO2.


Assuntos
Reatores Biológicos , Monóxido de Carbono , Fermentação , Hidrogênio , Concentração de Íons de Hidrogênio
4.
Gastrointest Endosc ; 2022 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-35500658

RESUMO

BACKGROUND AND AIMS: WingCap is a novel distal attachment device for colonoscopy that combines a cap and an existing mucosal exposure device, such as Endocuff or AmplifEye. We aimed to investigate whether WingCap-assisted colonoscopy can improve the adenoma detection rate (ADR) and adenoma per colonoscopy (APC) and simultaneously shorten caecal intubation time compared to standard colonoscopy. METHODS: We conducted a single-center prospective, randomized controlled trial for outpatients aged <18 y for colonoscopy. The primary outcome was ADR differences with the assistance of WingCap. Secondary outcomes included APC and other colonoscopy quality indicators, such as caecal intubation and withdrawal times. RESULTS: In total, 537 patients were randomized for WingCap-assisted or standard colonoscopy. Their mean age was 59.3 y, and 48.5% were male. ADR was significantly higher in the WingCap group than in the control group (37.2% vs. 26.6%, P=0.012). APC was greater with WingCap than with the standard (0.72 ± 1.34 vs. 0.45 ± 0.97, P=0.008), prominently for non-pedunculated (0.65 ± 1.25 vs. 0.42 ± 0.95, P=0.015), and for diminutive adenoma (0.42 ± 0.94 vs. 0.20 ± 0.64, P=0.002). With WingCap, ADR and APC significantly increased for beginner endoscopists, whereas they had a modest increase for experienced endoscopists. There were no differences in caecal intubation and withdrawal times between the two arms. There was no serious adverse event associated with the use of WingCap. CONCLUSIONS: WingCap-assisted colonoscopy was tolerable and efficacious for improving ADR and APC compared to standard colonoscopy, especially for non-pedunculated, diminutive adenomas and for beginner endoscopists.

5.
Parkinsons Dis ; 2021: 2268651, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34868542

RESUMO

BACKGROUND: Autonomic dysfunctions occur in the early stage of Parkinson's disease (PD) and impact the quality of life during the progression of the disease. In this study, we evaluated the serial progression of autonomic dysfunctions between different subtypes of a prospective PD cohort. MATERIALS AND METHODS: From the Parkinson's Progression Markers Initiative (PPMI) database, 325 PD patients (age: 61.2 ± 9.7, M : F = 215 : 110) were enrolled. Patients were subgrouped into tremor-dominant (TD), indeterminate, and postural instability and gait disorder (PIGD) subtypes. The progression of autonomic dysfunctions and dopaminergic denervation from I-123 FP-CIT SPECT images of each group were analyzed and compared at baseline, 12 months, 24 months, and 48 months of follow-up periods. RESULTS: The SCOPA-AUT score of the indeterminate subtype was significantly higher than that of the TD subtype (P < 0.05) at baseline and was significantly higher than that of both TD and PIGD subtypes (P < 0.05) at 48 months. The indeterminate subtype had the most significant correlation between the aggravation of dopaminergic denervation in I-123 FP-CIT SPECT images and the increase of SCOPA-AUT scores during 48 months of follow-up (r = 0.56, P < 0.01). CONCLUSIONS: Autonomic dysfunctions were most severe in the indeterminate subtype throughout the 48 months of the follow-up period, with a significant correlation with dopaminergic denervation. We suggest a positive relationship between dopaminergic denervation and autonomic dysfunctions of the indeterminate subtype, beginning from the early stage of PD.

6.
Anim Cells Syst (Seoul) ; 25(5): 272-282, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34745434

RESUMO

Zea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0.3%) by DNCB were performed on the dorsal skin and right ear of BALB/c mice to induce AD. Following FB801 and dexamethasone administered orally, the severity of skin lesions was examined macroscopically and histologically. Serum levels of immunoglobulin E (IgE) and various cytokines were determined by enzyme-linked immunosorbent assay. Oral administration of FB801 significantly reduced typical symptoms of AD (erythema/bleeding, swelling, molting/erosion and scaling/drying), scratching frequencies, and the recruitment of inflammatory and mast cells. In addition, FB801 suppressed serum levels of IgE and T helper (Th)2 type cytokines such as interleukin (IL)-4 and IL-10 in DNCB-treated BALB/c mice. Furthermore, FB801 reduced the degradation of inhibitor of nuclear factor-κB proteins (NF-κB) in tumor necrosis factor (TNF)-α-stimulated human keratinocyte (HaCaT) cells. These results suggest that FB801 inhibited the development of the AD-like skin symptoms by regulating Th1 and Th2 responses in the skin lesions in mice and suppressing TNF-α induced NF-κB activation in HaCaT cells, suggesting that FB801 has potential application as an effective alternative therapy for the prevention and management of AD.

7.
Micromachines (Basel) ; 12(10)2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34683213

RESUMO

Powerful sunlight, a high water temperature, and stagnation in the water flow induce eutrophication in rivers and lakes, which destroys the aquatic ecosystem and threatens the downstream water supply systems. Accordingly, it is very important to perform real-time measurements of nutrients that induce algal growth, especially total phosphorus, to preserve and manage the aquatic ecosystem. To conduct quantitative analysis of the total phosphorus in the aquatic ecosystem, it is essential to perform a pretreatment process and quickly separate the phosphorus, combined with organic and inorganic materials, into a phosphate. In this study, the sandblasting process was used for the physical etching of the wafer, and photocatalytic materials were deposited on the surface with various roughness in order to improve the photocatalytic reaction surface and efficiency. The photocatalytic reaction was applied to combine the pretreated sample with the coloring agent for color development, and the absorbance of the colored sample was analyzed quantitatively to compare and evaluate the characteristics, followed by the surface increase in the photocatalytic materials. In addition, the pretreatment and measurement parts were materialized in a single chip to produce a small and light total phosphorus analysis sensor.

8.
BMC Gastroenterol ; 21(1): 390, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34670529

RESUMO

BACKGROUND: Anti-tumor necrosis factor (TNF) treatment for inflammatory bowel disease (IBD) increases the risk of tuberculosis (TB) infection. In the present study, we analyzed the clinical characteristics and risks of TB in Korean patients with IBD who received anti-TNF treatment. METHODS: The study included patients with IBD who were treated using anti-TNF agents between January 2001 and June 2018 at the Asan Medical Center. Overall, 1434 patients with ulcerative colitis or Crohn's disease were enrolled. We calculated the incidence of active TB infection after anti-TNF treatment and compared the clinical characteristics of the TB group with those of the non-TB group. RESULTS: Twenty-one patients (1.46%) developed active TB infection, and the incidence rate of active TB was 366.73 per 100,000 person-years. In total, 198 patients (14.9%) were positive for latent tuberculosis infection (LTBI), of whom only eight (4%) did not complete LTBI treatment. The age at which the anti-TNF therapy was started was significantly higher in the TB group than in the non-TB group (HR 1.041, 95% CI 1.014-1.069, p = 0.002), and as age increased, so did the incidence rate of active TB infection (linearity p < 0.001). There was no significant difference in the incidence rate of LTBI between the TB and non-TB groups (HR 0.896, 95% CI 0.262-3.066, p = 0.862). CONCLUSIONS: In patients with IBD, the incidence rate of TB increased with age at anti-TNF therapy initiation. Active treatment of LTBI may lower the incidence of TB in patients with IBD who are to undergo anti-TNF therapy.


Assuntos
Doenças Inflamatórias Intestinais , Tuberculose , Estudos de Coortes , Hospitais , Humanos , Doenças Inflamatórias Intestinais/tratamento farmacológico , Doenças Inflamatórias Intestinais/epidemiologia , Infliximab/efeitos adversos , República da Coreia/epidemiologia , Tuberculose/epidemiologia , Inibidores do Fator de Necrose Tumoral
9.
Therap Adv Gastroenterol ; 14: 17562848211043017, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34539814

RESUMO

INTRODUCTION: Immunomodulators remain fundamental for the medical treatment of Crohn's disease (CD). Methotrexate (MTX) is widely used as a second-line immunomodulator; however, there is a lack of recent data on MTX monotherapy among the Asian population with CD. Therefore, in this study, we aimed to investigate the tolerability and clinical outcomes of MTX in Korean patients with CD. METHODS: A retrospective chart review was performed for CD patients treated with MTX monotherapy or in combination with 5-aminosalicylic acid (5-ASA), at the Asan Medical Center, Seoul, South Korea. The tolerability of MTX monotherapy within 6 months was assessed and the clinical effectiveness of MTX was evaluated based on the Crohn's disease activity index (CDAI). RESULTS: In total, 85 patients were included, of which 29 (34.1%) discontinued MTX due to intolerability during the follow-up. Adverse events (AEs) were reported in 41 (48.2%) patients. The most common AE was gastrointestinal disorders (17/41) and only one patient experienced a serious AE, a systemic infection that required hospitalization. Among the 56 patients who tolerated MTX within 6 months, 44 (65.9%) showed a clinical response. Moreover, no factor was significantly associated with intolerability. The administration method was the only factor significantly associated with a response to MTX (p = 0.041). The adjusted odds ratio of parenteral injection compared to oral administration was 5.68 (95% confidence interval (CI), 1.07-30.08). CONCLUSION: In this study, one-third of patients were intolerant to MTX; nonetheless, the response rate was as high as 65.9% among tolerant patients. In addition, no significant factors affected intolerability. In terms of the clinical response, parenteral injection could be better than oral administration.

10.
Sci Rep ; 11(1): 14002, 2021 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-34234193

RESUMO

Metformin increased cellular ROS levels in AsPC-1 pancreatic cancer cells, with minimal effect in HDF, human primary dermal fibroblasts. Metformin reduced cellular ATP levels in HDF, but not in AsPC-1 cells. Metformin increased AMPK, p-AMPK (Thr172), FOXO3a, p-FOXO3a (Ser413), and MnSOD levels in HDF, but not in AsPC-1 cells. p-AMPK and p-FOXO3a also translocated from the cytosol to the nucleus by metformin in HDF, but not in AsPC-1 cells. Transfection of si-FOXO3a in HDF increased ROS levels, while wt-FOXO3a-transfected AsPC-1 cells decreased ROS levels. Metformin combined with apigenin increased ROS levels dramatically and decreased cell viability in various cancer cells including AsPC-1 cells, with each drug used singly having a minimal effect. Metformin/apigenin combination synergistically decreased mitochondrial membrane potential in AsPC-1 cells but to a lesser extent in HDF cells. Metformin/apigenin combination in AsPC-1 cells increased DNA damage-, apoptosis-, autophagy- and necroptosis-related factors, but not in HDF cells. Oral administration with metformin/apigenin caused dramatic blocks tumor size in AsPC-1-xenografted nude mice. Our results suggest that metformin in cancer cells differentially regulates cellular ROS levels via AMPK-FOXO3a-MnSOD pathway and combination of metformin/apigenin exerts anticancer activity through DNA damage-induced apoptosis, autophagy and necroptosis by cancer cell-specific ROS amplification.


Assuntos
Antineoplásicos/farmacologia , Apigenina/farmacologia , Metformina/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Proteínas Quinases Ativadas por AMP/metabolismo , Trifosfato de Adenosina/metabolismo , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Fibroblastos , Proteína Forkhead Box O3/metabolismo , Humanos , Modelos Biológicos , Transdução de Sinais
11.
Anim Cells Syst (Seoul) ; 25(3): 161-170, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34262659

RESUMO

Glioblastoma multiforme (GBM) is the most aggressive primary brain cancer and this is due to cancer cells being apoptosis-resistant and having increased cell proliferation, migration, invasion, and angiogenesis properties. Previous studies have indicated both STAT and Notch pathways being important for initiation and progression in GBM. In this work, we first studied the effects of STAT inhibitors on Notch signalling using small molecule STAT inhibitors. It was observed that STAT inhibitors surprisingly activated Notch signalling by inducing NICD and Notch target genes in GBM cells. Thus, we aimed to combine STAT inhibitor treatment with a Notch pathway inhibitor and study effects on GBM tumourigenesis. STAT5 inhibitor (Pimozide) and STAT3 inhibitor (S3I-201) were individually used in combination with γ-secretase inhibitor (DAPT), an inhibitor of Notch signalling, in a panel of GBM cells for cell proliferation and epithelial plasticity changes. Compared with single-agent treatments, combinatorial treatments with the STAT and Notch inhibitors significantly increased apoptosis in the treated cells, impairing cell proliferation, migration, and invasion. These findings suggest that concurrent blocking of STAT and Notch signalling pathways could provide added therapeutic benefit for the treatment of glioblastoma.

12.
Energy (Oxf) ; 231: 120915, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34054202

RESUMO

COVID-19 made considerable changes in the lifestyle of people, which have led to a rise in energy use in homes. So, this study investigated the relationship between COVID-19 and domestic hot water demands. For this purpose, a nondimensional and principal component analysis were conducted to find out the influencing factors using demand data before and after COVID-19 from our study site. Analysis showed that the COVID-19 outbreak affected the daily peak time and the amount of domestic hot water usage, the active case number of COVID-19 was a good indicator for correlating the changes in hot water demand and patterns. Based on this, a machine learning model with an artificial neural network was developed to predict hot water demand depending on the severity of COVID-19 and the relevant correlation was also derived. The model analysis showed that the increase in the number of active cases in the region affected the hot water demand increased at a certain rate and the maximum demand peak in morning during weekdays and weekends decreased. Furthermore, if the number of active cases reached more than 4000, the peak in morning moved to afternoon so that the energy use patterns of weekdays and weekends are assimilated.

13.
Eur Arch Otorhinolaryngol ; 278(11): 4569-4574, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34032908

RESUMO

PURPOSE: Little is known about the incidence of thyroid cancer in patients with obstructive sleep apnea (OSA). This study aimed to evaluate whether OSA is associated with the incidence of thyroid cancer based on the Korea National Health Insurance Service (KNHIS) database. METHODS: This study was designed as a retrospective cohort data analysis of the KNHIS dataset. A total of 198,574 patients who were over 20 years of age and had been newly diagnosed with OSA between 2007 and 2014 were enrolled. A control group of 992,870 individuals was selected based on propensity score matching by age and sex. The mean follow-up duration was 4.5 ± 2.3 years. The primary endpoint was the incidence of newly diagnosed thyroid cancer. RESULTS: The hazard ratio (HR) for thyroid cancer incidence among OSA patients compared to the control was 1.72 (95% confidence interval [CI] 1.60-1.84) based on Model 1 (not adjusted by any covariate) and 1.64 (95% CI 1.53-1.76) based on Model 2 (adjusted by income level, diabetes, hypertension, and dyslipidemia). Thyroid cancer incidence was significantly higher in male patients (HR = 1.93, 95% CI 1.74-2.12) than female ones (HR = 1.39, 95% CI 1.26-1.54). When compared by age, the HR of thyroid cancer was higher in middle-aged (40 ≤ age < 65 years) patients (HR = 1.68, 95% CI 1.55-1.83) than in young (20 ≤ age < 40 years, HR = 1.53, 95% CI 1.32-1.77) or old (65 ≤ age, HR = 1.28, 95% CI 0.94-1.74) patients. CONCLUSION: OSA may increase the risk of developing thyroid cancer, especially in middle-aged men.


Assuntos
Apneia Obstrutiva do Sono , Neoplasias da Glândula Tireoide , Adulto , Idoso , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Programas Nacionais de Saúde , República da Coreia/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Apneia Obstrutiva do Sono/epidemiologia , Neoplasias da Glândula Tireoide/epidemiologia
14.
Artigo em Inglês | MEDLINE | ID: mdl-33672896

RESUMO

Many studies have been conducted regarding the association between night work and diabetes, but the association between impaired fasting glucose (IFG) and night work is still unclear. The aim of this study was to evaluate this association using the Special Health Examination (SHEW) for Korean night workers. Laboratory, questionnaire, and physical examination data were collected for 80,077 manual workers between 2014 and 2016 from Korea Medical Institute, and associations of the data with IFG were evaluated using a multivariate logistic regression model. The odds ratios for IFG among those who worked night shifts for 2~5 years, 5~12 years, and 12 years or over (ref: <2 years) after adjusting for abdominal obesity were 1.14 (0.90-1.45), 1.41 (1.10-1.81), and 1.75 (1.41-2.19), respectively. A dose-response relationship was identified between the duration of night work and the prevalence of IFG (p for trend <0.05). A dose relationship remained significant when a subgroup of non-obese participants was analyzed. We identified an association and a dose-response relationship between the number of years of night work and IFG. To prevent the development of diabetes in night workers, we suggest that they should be pre-emptively screened and treated from the stage of IFG.


Assuntos
Diabetes Mellitus Tipo 2 , Jejum , Glicemia , Estudos Transversais , Humanos , Prevalência , República da Coreia/epidemiologia , Fatores de Risco
15.
Anim Cells Syst (Seoul) ; 25(1): 65-73, 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33717418

RESUMO

Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.

16.
Int J Med Sci ; 18(7): 1580-1591, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33746574

RESUMO

Melanotransferrin (CD228), firstly reported as a melanoma-associated antigen, is a membrane-bound glycoprotein of an iron-binding transferrin homolog. CD228 was found to be expressed significantly higher in human bone marrow-derived mesenchymal stem cells (hBM-MSC) than in human embryonic fibroblasts (FB) by RT-PCR, western blotting and flow cytometry. The expression of CD228 declined in aged hBM-MSC as osteogenesis-related genes did. We examined a possible role for CD228 in the regulation of osteogenesis and adipogenesis of hBM-MSC. Surprisingly, siRNA-mediated CD228 knockdown increased the expression of the transcription factor DLX5 and enhanced osteogenesis of hBM-MSC evidenced by an increased expression of the runt-related transcription factor 2 (RUNX2), osterix (Osx), and osteocalcin (OC), as well as higher alkaline phosphatase (ALP) activity and extracellular calcium deposition. Interestingly, hBM-MSC transfected with CD228 siRNA also showed an increase in intracellular lipid level during adipogenesis, indicated by oil red O staining of differentiated adipocytes. Overall, our study unveils CD228 as a cell surface molecule expressed by young hBM-MSC, but not by FB. It also provides evidence to suggest a role for CD228 as a negative regulator of osteogenesis and of lipid accumulation during adipogenesis in hBM-MSC in vitro.


Assuntos
Adipogenia/genética , Diferenciação Celular/genética , Glicoproteínas de Membrana/metabolismo , Células-Tronco Mesenquimais/fisiologia , Osteogênese/genética , Linhagem Celular , Embrião de Mamíferos , Fibroblastos , Regulação da Expressão Gênica no Desenvolvimento , Técnicas de Silenciamento de Genes , Proteínas de Homeodomínio/metabolismo , Humanos , Glicoproteínas de Membrana/genética , Osteocalcina/metabolismo , Fator de Transcrição Sp7/metabolismo , Fatores de Transcrição/metabolismo
17.
J Food Sci Technol ; 58(1): 389-396, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33505084

RESUMO

Kimchi is a traditional Korean fermented vegetable, which is also widely consumed in Japan and China. However, little is known about the kimchi proteome. In this study, Korean and Chinese kimchi proteomes were evaluated by shotgun proteomics. A total of 250 proteins were annotated, and 29 of these were expressed at > 1% of the average relative abundance. Discrimination of the geographical origins of Korean and Chinese kimchi samples was possible using multivariate analysis of the proteomic data, and 23 proteins were expressed differently between the two types (p < 0.001), and represent possible markers to discriminate between Chinese and Korean kimchi. This study provides important insights into the kimchi proteome and illustrates the proteomic differences caused by geographical origin.

18.
Anim Cells Syst (Seoul) ; 24(3): 143-150, 2020 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-33209194

RESUMO

Chrysin, a natural flavonoid, is the main ingredient of many medicinal plants, which shows potent pharmacological properties. In the present study, the antinociceptive effects of chrysin were examined in ICR mice. Chrysin orally administered at the doses of from 10 to 100 mg/kg exerted the reductions of formalin-induced pain behaviors observed during the second phase in the formalin test in a dose-dependent manner. In addition, the antinociceptive effect of chrysin was further characterized in streptozotocin-induced diabetic neuropathy model. Oral administration chrysin caused reversals of decreased pain threshold observed in diabetic-induced peripheral neuropathy model. Intraperitoneally (i.p.) pretreatment with naloxone (a classic opioid receptor antagonist), but not yohimbine (an antagonist of α2-adrenergic receptors) or methysergide (an antagonist of serotonergic receptors), effectively reversed chrysin-induced antinociceptive effect in the formalin test. Moreover, chrysin caused a reduction of formalin-induced up-regulated spinal p-CREB level, which was also reversed by i.t. pretreated naloxone. Finally, chrysin also suppressed the increase of the spinal p-CREB level induced by diabetic neuropathy. Our results suggest that chrysin shows an antinociceptive property in formalin-induced pain and diabetic neuropathy models. In addition, spinal opioid receptors and CREB protein appear to mediate chrysin-induced antinociception in the formalin-induced pain model.

19.
PLoS One ; 15(11): e0241598, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33180841

RESUMO

The association between obstructive sleep apnea (OSA) and malignant brain tumors has yet to be fully investigated. Therefore, the purpose of this study was to elucidate the effect of OSA on brain tumor incidence based on the Korea National Health Insurance Service (KNHIS) dataset. The KNHIS data between 2007 and 2014 were analyzed, and the primary endpoint was newly diagnosed malignant brain tumor. A total of 198,574 subjects aged ≥ 20 years with newly diagnosed OSA were enrolled in the study, and 992,870 individuals were selected as a control group based on propensity score matching (PSM) by gender and age. The average follow-up duration was 4.8 ± 2.3 years. The hazard ratios (HRs) for brain tumor for patients with OSA were 1.78 (95% confidence interval [CI]: 1.42-2.21) in Model 1 (not adjusted with any covariate) and 1.67 (95% CI: 1.34-2.09) in Model 2 (adjusted for income level, diabetes, hypertension, dyslipidemia, and COPD). In subgroup analysis by gender, the odds ratios (OR) of OSA were 1.82 (95% CI: 1.41-2.33) in men and 1.26 (95% CI: 0.74-2.03) in women. The ORs were 1.97 (95% CI: 1.15-3.24) in the older (age ≥ 65 years) group, 1.66 (95% CI: 1.25-2.17) in the middle-aged (40 ≤ age < 65 years) group, and 1.41 (0.78-2.44) in the young (20 ≤ age < 40 years) group. In conclusion, OSA may increase the incidence of brain tumors.


Assuntos
Neoplasias Encefálicas/epidemiologia , Apneia Obstrutiva do Sono/epidemiologia , Adulto , Idoso , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Programas Nacionais de Saúde/estatística & dados numéricos , República da Coreia , Apneia Obstrutiva do Sono/complicações , Fatores Socioeconômicos
20.
Anal Methods ; 12(48): 5767-5800, 2020 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-33241791

RESUMO

A great deal of effort has been expended to develop accurate means of determining the properties of synthetic polymers using matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS). Many studies have focused on the importance of sample pre-treatment to obtain accurate analysis results. This review discusses the history of synthetic polymer characterization and highlights several applications of MALDI-TOF MS that recognize the importance of pre-treatment technologies. The subject area is of significance in the field of analytical chemistry, especially for users of the MALDI technique. Since the 2000s, many such technologies have been developed that feature improved methods and conditions, including solvent-free systems. In addition, the recent diversification of matrix types and the development of carbon-based matrix materials are described herein together with the current status and future directions of MALDI-TOF MS hardware and software development. We provide a summary of processes used for obtaining the best analytical results with synthetic polymeric materials using MALDI-TOF MS.

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