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1.
Talanta ; 221: 121606, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076136

RESUMO

Due to favourable efficiency and low cost, dual-response fluorescent probes play critical roles in the development of fluorescence assay system. Herein, a novel dual-response fluorescent probe (RDCN) was designed and synthesized for the detection of two environmental contaminants: hydrazine (N2H4) and cyanide (CN-). Probe RDCN exhibited discriminative sensing behaviors to N2H4 and CN- with different reaction mechanisms, allowing the high selectivity and sensitivity detection for N2H4 and CN-. The probe itself displayed red-emitting fluorescence as a result of strong intramolecular charge transfer (ICT) between diethylamino and dicyano. After mixing with hydrazine, a new corresponding hydrazone came into being with an intense yellow fluorescence. While, the probe could also largely switch to blue fluorescence in response to CN-. Furthermore, the probe RDCN was successfully employed to determine N2H4 and CN- in water samples with the detection limits of 0.08 µM and 0.33 µM, and to visualize N2H4 and CN- in live cells by means of different fluorescence channels (blue and yellow channels), revealing that probe RDCN has potential applications for discriminative detection N2H4 and CN- in biological environment.

2.
Food Chem ; 337: 127755, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32777567

RESUMO

Since the beginning of the widespread use of pesticides, their removal from food has become a serious concern. In this study, the removal of residual pesticides (malathion and carbosulfan) from pak choi via treatment with ozonated water was investigated. Under the optimal treatment conditions, i.e., 2.0 mg/L ozonated water and a treatment duration of 15 min, malathion and carbosulfan were degraded by 53.0 and 33.0%, respectively, without any significant changes in color. Even though there was a slight decrease in vitamin C content (~7.9 mg/100 g) following the treatments, a significant decrease in the microbial colonies on the vegetables was observed. Additionally, the pesticide degradation mechanism showed good fitting with a "first + first"-order kinetic model (R2 > 0.9), and the slope (k) indicated that ozone had a more prominent degradation effect on malathion than on carbosulfan. Therefore, this study provides a theoretical basis for controlling agricultural pesticide residues in household applications.

3.
Int Immunopharmacol ; 89(Pt B): 107080, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33069926

RESUMO

OBJECTIVE: Identify the prognostic value and investigate the association between programmed cell death 1 (PDCD1) gene expression and immune infiltration in pan-cancer. METHODS: We used a series of bioinformatics methods to comprehensively analyze the relationship between PDCD1 gene and prognosis, tumor mutational burden (TMB), microsatellite instability (MSI), immune cell infiltration of various cancers from the existing public database, and try to find the potential prognostic value of PDCD1 for in pan-cancer. RESULTS: High expression of PDCD1 was closely related to better overall survival (OS) and disease-specific survival (DSS) in breast invasive carcinoma, head and neck squamous cell carcinoma, skin cutaneous melanoma, and uterine corpus endometrial carcinoma; have a better disease-free interval (DFI) and progression-free interval (PFI) in several cancer types. Meanwhile, the high level of PDCD1 gene expression was associated with poorer OS, DSS, and PFI in brain lower grade glioma and uveal melanoma; poorer OS in acute myeloid leukemia and kidney renal papillary cell carcinoma; poorer OS and DSS in glioblastoma multiforme; poorer DSS in kidney renal clear cell carcinoma, by Kaplan-Meier and Cox survival analysis. PDCD1 gene expression was significantly correlated with TMB and MSI in 14 and 12 cancer types, respectively, and infiltrating levels of immune cells, especially Macrophages M0, M1, CD4-T-cells, CD8-T-cells, and T cells follicular helper, in most of eight cancer types. CONCLUSION: PDCD1 can be used as a prognostic marker in multiple cancers, owing to it is closely associated with TMB, MSI, and immune cells infiltration.

4.
Water Res ; 188: 116479, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33069949

RESUMO

Peracetic acid (PAA) has attracted growing attention as an alternative oxidant and disinfectant in wastewater treatment due to the increased demand to reduce chlorine usage and control disinfection byproducts (DBPs). These applications have stimulated new investigations on PAA-based advanced oxidation processes (AOPs), which can enhance water disinfection and remove micropollutants. The purpose of this review is to conduct a comprehensive analysis of scientific information and experimental data reported in recent years on the applications of PAA-based AOPs for the removal of chemical and microbiological micropollutants from water and wastewater. Various methods of PAA activation, including the supply of external energy and metal/metal-free catalysts, as well as their activation mechanisms are discussed. Then, a review on the usage of PAA-based AOPs for contaminant degradation is given. The degradation mechanisms of organic compounds and the influence of the controlling parameters of PAA-based treatment systems are summarized and discussed. Concurrently, the application of PAA-based AOPs for water disinfection and the related mechanisms of microorganism inactivation are also reviewed. Since combining UV light with PAA is the most commonly investigated PAA-based AOP for simultaneous pathogen inactivation and micropollutant oxidation, we have also focused on PAA microbial inactivation kinetics, together with the effects of key experimental parameters on the process. Moreover, we have discussed the advantages and disadvantages of UV/PAA as an AOP against the well-known and established UV/H2O2. Finally, the knowledge gaps, challenges, and new opportunities for research in this field are discussed. This critical review will facilitate an in-depth understanding of the PAA-based AOPs for water and wastewater treatment and provide useful perspectives for future research and development for PAA-based technologies.

5.
Colloids Surf B Biointerfaces ; 197: 111378, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33010719

RESUMO

Cancer immunotherapy is an important tumor therapy option for prevention and treatment of tumors and has attracted tremendous interests. However, the therapeutic outcomes are limited by insufficient antigen uptake and presentation by antigen-presenting cells such as dendritic cells (DCs). In this study, mannose-functionalized antigen nanoparticles with endosome escape activity were designed for targeted DCs, accelerated endosomal escape and enhanced MHC-I antigen presentation for cancer immunotherapy. Mannose was selected as DCs targeting ligand to enhance antigen uptake. Model antigen ovalbumin (OVA) was directly conjugated with mannose to obtain DCs targeting antigen, which was then complexed with polyethylenimine (PEI) through electrostatic interaction to form mannose-functionalized antigen nanoparticles (MAN-OVA/PEI NPs). Flow cytometry analysis revealed that the MAN-OVA/PEI NPs greatly increased antigen uptake by DCs compared with OVA/PEI NPs. Confocal laser scanning microscopy further demonstrated that MAN-OVA/PEI NPs enhanced cytosolic antigen release. Moreover, MAN-OVA/PEI NPs significantly promoted cytokine production and DCs maturation in vitro. More importantly, MAN-OVA/PEI NPs treated DCs exhibited enhanced cross-presentation to B3Z T cell hybridoma in vitro. This work suggests that mannose-functionalized antigen nanoparticles provide a versatile delivery vehicle for targeted DCs, accelerated endosomal escape and enhanced MHC-I antigen presentation for cancer immunotherapy.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33017289

RESUMO

For 360° video, the existing visual quality assessment (VQA) approaches are designed based on either the whole frames or the cropped patches, ignoring the fact that subjects can only access viewports. When watching 360° video, subjects select viewports through head movement (HM) and then fixate on attractive regions within the viewports through eye movement (EM). Therefore, this paper proposes a two-staged multi-task approach for viewport-based VQA on 360° video. Specifically, we first establish a large-scale VQA dataset of 360° video, called VQA-ODV, which collects the subjective quality scores and the HM and EM data on 600 video sequences. By mining our dataset, we find that the subjective quality of 360° video is related to camera motion, viewport positions and saliency within viewports. Accordingly, we propose a viewport-based convolutional neural network (V-CNN) approach for VQA on 360° video, which has a novel multi-task architecture composed of a viewport proposal network (VP-net) and viewport quality network (VQ-net). The VP-net handles the auxiliary tasks of camera motion detection and viewport proposal, while the VQ-net accomplishes the auxiliary task of viewport saliency prediction and the main task of VQA. The experiments validate that our V-CNN approach significantly advances state-of-the-art VQA performance on 360° video and it is also effective in the three auxiliary tasks.

7.
Environ Sci Technol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33064949

RESUMO

Scale formation is an important challenge in water and wastewater treatment systems. However, due to the complex nature of membrane surfaces, the effects of specific membrane surface characteristics on scale formation are poorly understood. In this study, the independent effect of surface hydrophobicity on gypsum (CaSO4·2H2O) scale formation via surface-induced nucleation and bulk homogeneous nucleation was investigated using quartz crystal microbalance with dissipation (QCM-D) on self-assembled monolayers (SAMs) terminated with -OH, -CH3, and -CF3 functional groups. Results show that higher surface hydrophobicity enhances both surface-induced nucleation of gypsum and attachment of gypsum crystals formed from homogeneous nucleation in the bulk solution. The enhanced surface-induced nucleation is attributed to the lower nucleation energy barrier on a hydrophobic surface, while the increased gypsum crystal attachment results from the favorable hydrophobic interactions between gypsum and more hydrophobic surfaces. Contrary to previous findings, the role of Ca2+ adsorption in surface-induced nucleation was found to be relatively small and similar on the different SAMs. Therefore, increasing material hydrophilicity is a potential approach to reduce gypsum scaling.

8.
J Cancer Educ ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068265

RESUMO

Patient knowledge of risk factors, signs and symptoms associated with oral cancers is crucial for increasing the likelihood of patient presentation for opportunistic screening and reducing delay in patient appraisal for early detection. This study aimed to assess the knowledge of oral cancer and to ascertain socio-demographic factors that influence knowledge amongst adult dental patients attending public clinics in Brisbane, Australia. A convenience sample of 213 adult dental patients who attended the Herston and Stafford public health clinics in Brisbane, Australia, between July and August 2019 participated in the self-administered questionnaire. Multivariate analyses were performed to identify predictors for oral cancer knowledge. Patients were well informed of smoking as a risk factor (n = 135; 84.4%), whereas only 53.8% (n = 82) of participants agreed that heavy alcohol consumption was a risk factor. A larger proportion of participants identified difficulty of moving the tongue (n = 79; 49.4%) and pain on swallowing (n = 72; 45.0%) compared to the proportion who identified fixed red patches (n = 61; 38.1%) and fixed white patches (n = 57; 35.6%) as a sign or symptom. Education level and gender were significant knowledge predictors for alcohol (p = 0.01), old age (p = 0.008) and family history (p = 0.004) as a risk factors for oral cancer. Those with a family history of cancer were more likely to identify a red patch (p = 0.02), bleeding gums (p = 0.001) and altered sensation (p = 0.023) as a sign or symptom of oral cancer. Overall, patient knowledge was greater for risk factors than for signs and symptoms for oral cancer. Symptoms associated with later stages of cancer were recognised by a greater proportion of patients compared to early stages of oral cancer. These results indicate the need for targeted public health initiatives to improve patient knowledge.

9.
J Virol ; 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028719

RESUMO

The low fidelity of foot-and-mouth disease virus (FMDV) RNA-dependent RNA polymerase allows FMDV to exhibit high genetic diversity. Previously, we have shown that the genetic diversity of FMDV plays an important role in virulence in suckling mice. Here, we mutated the amino acid residue Phe257, located in the finger domain of FMDV polymerase and conserved across FMDV serotypes, to a cysteine (F257C) to study the relationship between viral genetic diversity, virulence, and transmissibility in natural hosts. The single amino acid substitution in FMDV polymerase resulted in a high-fidelity virus variant, rF257C, with growth kinetics indistinguishable from wild-type virus in cell culture but displayed smaller plaques and impaired fitness in direct competition assays. Furthermore, we found that the rF257C was attenuated in vivo in both suckling mice and pigs, one of its natural hosts. Importantly, contact-exposure experiments showed that the rF257C virus exhibited reduced transmissibility than wild-type FMDV in the porcine model. This study provides evidence that FMDV genetic diversity is important for viral virulence and transmissibility in susceptible animals. Given that type O FMDV exhibits the highest genetic diversity among all the seven serotypes of FMDV, we propose that the lower polymerase fidelity of the type O FMDV could contribute to its dominance worldwide.IMPORTANCE Among the seven serotypes of FMDV, serotype O FMDV has the broadest distribution worldwide, which could be due to their high virulence and transmissibility induced by the high genetic diversity. In this paper, we generated a single amino acid substitution FMDV variant with a high-fidelity polymerase associated with viral fitness, virulence, and transmissibility in a natural host. The results highlight that maintenance of viral population diversity is essential for interhost viral spread. This study provides evidence that higher genetic diversity of type O FMDV could increase both virulence and transmissibility, thus leading to their dominance in the global epidemic.

10.
Zootaxa ; 4852(5): zootaxa.4852.5.7, 2020 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-33056399

RESUMO

A new species of Neocerambyx Thomson, 1860 is described, N. guangxiensis sp. nov., from Guangxi, China. The description and photographs of adult habitus and male genitalia of this new species are provided.


Assuntos
Besouros , Animais , China , Genitália Masculina , Masculino
11.
BMC Gastroenterol ; 20(1): 348, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33076834

RESUMO

BACKGROUND: Previous studies have demonstrated the benefits of thymosin alpha-1 (Tα1) in anti-virus, immunological enhancement and anti-inflammation. However, it is controversial about the efficacy and safety of entecavir (ETV) plus Tα1 combination therapy versus ETV monotherapy in cirrhotic patients with hepatitis B virus (HBV) infection. METHODS: The systematic review and meta-analysis of randomized clinical trials (RCTs) were performed to evaluate the efficacy and safety of ETV plus Tα1 combination therapy versus ETV monotherapy in HBV-related patients with cirrhosis. We performed a systematic literature search via PubMed, Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL), EMBASE, China National Knowledge Infrastructure (CNKI), Chinese Science and Technology Journals Database (VIP), and Chinese Biological Medicine database (CBM). Relative risk (RR) and standardized mean difference (SMD) with a fixed- or random- effect model were calculated. Heterogeneity was assessed through a Cochrane Q-test and I2 values. RESULTS: Seven RCTs involving 1144 subjects were included in the systematic review and meta-analysis. Compared with ETV monotherapy, ETV plus Tα1 combination therapy led to a higher complete response (RR = 1.18; 95% CI, 1.07-1.30). In post treatment for 24 weeks, the HBV DNA undetectable rate and HBeAg loss rate were higher in ETV plus Tα1 group than in ETV alone group (RR = 1.91; 95% CI, 1.56-2.35; RR = 2.05; 95% CI, 1.62-2.60). However, after 48 and 52 weeks of treatment, there was no significant difference between the combination therapy and ETV monotherapy (RR = 1.07; 95% CI, 0.96-1.18; RR = 1.17; 95% CI, 0.89-1.55). At week 52 of treatment, the HBsAg loss rate of ETV plus Tα1 group was no significance with that of ETV alone group (RR = 1.03; 95% CI, 0.15-7.26). In comparison with ETV alone, the some biochemical parameters and liver fibrosis were obviously improved by ETV plus Tα1, and there was significant heterogeneity. In addition, the number of adverse events was significantly reduced by ETV plus Tα1, compared to ETV alone (RR = 0.48; 95% CI, 0.24-0.95). CONCLUSIONS: ETV plus Tα1 might lead to a higher clinical response and a lower comprehensive adverse reaction rate in HBV-related patients with cirrhosis, compared to ETV alone. However, the whole patients included in this meta-analysis were from Chinese mainland, so that more worldwide RCTs with a larger sample size are needed to verify the current findings.

12.
Chem Commun (Camb) ; 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33079080

RESUMO

Rhamnose and sTn antigen were co-conjugated to bovine serum albumin (BSA), a weakly immunogenic carrier protein, for cancer vaccine development. The immune responses against sTn have been significantly augmented with the involvement of Rha-specific antibodies to enhance antigen uptake.

13.
Front Immunol ; 11: 2001, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013854

RESUMO

Background: X-linked agammaglobulinemia (XLA) is caused by a mutation of the Bruton's tyrosine kinase (BTK) gene and is the most common genetic mutation in patients with congenital agammaglobulinemia. The aim of this study was to analyze the clinical features, genetic defects, and/or BTK expression in patients suspected of having XLA who were referred from the Taiwan Foundation of Rare Disorders (TFRD). Methods: Patients with recurrent bacterial infections in the first 2 years of life, serum IgG/A/M below 2 standard deviations of the normal range, and ≦2% CD19+B cells were enrolled during the period of 2004-2019. The frequency of infections, pathogens, B-lymphocyte subsets, and family pedigree were recorded. Peripheral blood samples were sent to our institute for BTK expression and genetic analysis. Results: Nineteen (from 16 families) out of 29 patients had BTK mutations, including 7 missense mutations, 7 splicing mutations, 1 nonsense mutation, 2 huge deletions, and 2 nucleotide deletions. Six novel mutations were detected: c.504G>T [p.K168N], c.895-2A>G [p.Del K290 fs 23*], c.910T>G [p.F304V], c.1132T>C [p.T334H], c.1562A>T [p.D521V], and c.1957delG [Del p.D653 fs plus 45 a.a.]. All patients with BTK mutations had obviously decreased BTK expressions. Pseudomonas sepsis developed in 14 patients and led to both Shanghai fever and recurrent hemophagocytic lymphohistiocytosis (HLH). Recurrent sinopulmonary infections and bronchiectasis occurred in 11 patients. One patient died of pseudomonas sepsis and another died of hepatocellular carcinoma before receiving optimal treatment. Two patients with contiguous gene deletion syndrome (CGS) encompassing the TIMM8A/DDP1 gene presented with early-onset progressive post-lingual sensorineural Deafness, gradual Dystonia, and Optic Neuronopathy syndrome (DDON) or Mohr-Tranebjaerg syndrome (MTS). Conclusion: Pseudomonas sepsis was more common (74%) than recurrent sinopulmonary infections in Taiwanese XLA patients, and related to Shanghai fever and recurrent HLH, both of which were prevented by regular immunoglobulin infusions. Approximately 10% of patients belonged to CGS involving the TIMM8A/DDP1 gene and presented with the DDON/MTS phenotype in need of aggressive psychomotor therapy.

14.
Front Immunol ; 11: 2133, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013900

RESUMO

The small GTPase Rab5 is one of the master regulators of vesicular trafficking that participates in early stages of the endocytic pathway, such as endocytosis and endosome maturation. Three Rab5 isoforms (a, b, and c) share high sequence identity, and exhibit complex functions. However, the role of Rab5c in virus infection and cellular immune responses remains poorly understood. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected grouper spleen (GS) cells, we investigated the role of Rab5c in virus infection and host immune responses. Rab5c was cloned from the orange-spotted grouper, Epinephelus coioides, and termed EcRab5c. EcRab5c encoded a 220-amino-acid polypeptide, showing 99% and 91% identity to Anabas testudineus, and Homo sapiens, respectively. Confocal imaging showed that EcRab5c localized as punctate structures in the cytoplasm. However, a constitutively active (CA) EcRab5c mutant led to enlarged vesicles, while a dominant negative (DN) EcRab5c mutant reduced vesicle structures. EcRab5c expression levels were significantly increased after SGIV infection. EcRab5c knockdown, or CA/DN EcRab5c overexpression significantly inhibited SGIV infection. Using single-particle imaging analysis, we further observed that EcRab5c disruption impaired crucial events at the early stage of SGIV infection, including virus binding, entry, and transport from early to late endosomes, at the single virus level. Furthermore, it is the first time to investigate that EcRab5c is required in autophagy. Equally, EcRab5c positively regulated interferon-related factors and pro-inflammatory cytokines. In summary, these data showed that EcRab5c exerted a bi-functional role on iridovirus infection and host immunity in fish, which furthers our understanding of virus and host immune interactions.

15.
J Child Health Care ; : 1367493520966682, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33021394
16.
Artigo em Inglês | MEDLINE | ID: mdl-33029929

RESUMO

A catalytic asymmetric alkylation of azoles with secondary 1-arylalkyl bromides through direct C-H functionalization is reported. Under blue light photoexcitation, copper(I)/carbazole-based bisoxazoline (CbzBox) catalytic system exhibits good reactivity and high stereoselectivity, thus offering an efficient strategy for constructing chiral alkyl azoles.

17.
ACS Nano ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33034444

RESUMO

Owning triply periodic minimal surfaces and three-dimensional (3D) interconnected pores, bicontinuous porous materials have drawn enormous attention due to their great academic interest and potential applications in many fields including energy and catalysis. However, their synthesis has remained a great challenge. Here, we demonstrate the synthesis of a bicontinuous porous organic semiconductor photocatalyst, which involves the preparation of SiO2 with a shifted double diamond (DD) structure through solvent evaporation-induced self-assembly of a polystyrene-block-poly(ethylene oxide) diblock copolymer and tetraethyl orthosilicate, followed by SiO2-templated self-condensation of melamine monomers in a vacuum. Strikingly, the resultant DD-structured graphitic carbon nitride (g-CN) possesses two sets of 3D continuous mesopores with a mean diameter of 14 nm, which afford a high specific surface area of 131 m2 g-1 and an optical band gap of 2.8 eV. Being a visible-light-driven photocatalyst, the bicontinuous mesoporous g-CN exhibits high catalytic activity for water splitting to generate H2 (6831 µmol g-1 h-1) with excellent cycling stability. This study provides a protocol for the construction of ordered mesoporous materials containing 3D continuous channels, which holds promise for catalysis applications.

18.
J Stroke Cerebrovasc Dis ; 29(11): 105154, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33066884

RESUMO

OBJECTIVE: Transcranial color-duplex sonography (TCCS) is a promising method in evaluating the hemodynamics in patients with moyamoya disease (MMD). This study aimed to explore the feasibility of preoperative TCCS in predicting the outcome of revascularization surgery in MMD patients. METHODS: We retrospectively analysed 64 cases of MMD patients receiving revascularization surgery from January 2012 to January 2014. We utilized TCCS to perform comprehensive hemodynamic examination on the hemodynamics of bilateral intracranial and extracranial cerebrovascular flow and assessed the surgical outcomes and prognosis through the longitudinal comparison of the preoperative and postoperative cerebrovascular hemodynamics. Occurrence of bypass blockage was regarded as surgical failure. RESULTS: We established a prediction model for bypass blockage among MMD patients with an AUC of 0.858 (95% CI: 0.666-1). The parameters, EDV of ECA and PSV of MA obtained by the model are the main preoperative predictors for bypass blockage. CONCLUSIONS: TCCS could preoperatively determine the degree of MMD and evaluate the outcome of revascularization surgery. It also is a feasible tool to predict the curative effect by providing preoperative hemodynamic information.

19.
J Diabetes Res ; 2020: 6873891, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33029536

RESUMO

Background: An estimated 425 million people globally have diabetes, accounting for 12% of the world's health expenditures, and the number continues to grow, placing a huge burden on the healthcare system, especially in those remote, underserved areas. Methods: A total of 584,168 adult subjects who have participated in the national physical examination were enrolled in this study. The risk factors for type II diabetes mellitus (T2DM) were identified by p values and odds ratio, using logistic regression (LR) based on variables of physical measurement and a questionnaire. Combined with the risk factors selected by LR, we used a decision tree, a random forest, AdaBoost with a decision tree (AdaBoost), and an extreme gradient boosting decision tree (XGBoost) to identify individuals with T2DM, compared the performance of the four machine learning classifiers, and used the best-performing classifier to output the degree of variables' importance scores of T2DM. Results: The results indicated that XGBoost had the best performance (accuracy = 0.906, precision = 0.910, recall = 0.902, F-1 = 0.906, and AUC = 0.968). The degree of variables' importance scores in XGBoost showed that BMI was the most significant feature, followed by age, waist circumference, systolic pressure, ethnicity, smoking amount, fatty liver, hypertension, physical activity, drinking status, dietary ratio (meat to vegetables), drink amount, smoking status, and diet habit (oil loving). Conclusions: We proposed a classifier based on LR-XGBoost which used fourteen variables of patients which are easily obtained and noninvasive as predictor variables to identify potential incidents of T2DM. The classifier can accurately screen the risk of diabetes in the early phrase, and the degree of variables' importance scores gives a clue to prevent diabetes occurrence.

20.
Sci Rep ; 10(1): 18193, 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33097806

RESUMO

Various therapeutic platforms have been developed for repairing bone defects. However, scaffolds possess both cortical bone-matching mechanical properties and excellent osteoconductivity for load-bearing bone defects repair is still challenging in the clinic. In this study, inspired by the structure of the ferroconcrete, a high-strength bifunctional scaffold has been developed by combining surface-modified magnesium alloy as the internal load-bearing skeleton and bioglass-magnesium phosphate bone cement as the osteoconductive matrix. The scaffold combines the high mechanical strength and controllable biodegradability of surface-modified magnesium alloy with the excellent biocompatibility and osteoconductivity of bioglass-magnesium phosphate bone cement, thus providing support for load-bearing bone defects and subsequently bone regeneration. The scaffolds generate hydroxyapatite (HA) during the degrading in simulated body fluid (SBF), with the strength of the scaffold decreasing from 180 to 100 MPa in 6 weeks, which is still sufficient for load-bearing bone. Moreover, the scaffolds showed excellent osteoconductivity in vitro and in vivo. In a New Zealand White Rabbit radius defect model, the scaffolds degrade gradually and are replaced by highly matured new bone tissues, as assessed by image-based analyses (X-ray and Micro-CT) and histological analyses. The bone formation-related proteins such as BMP2, COL1a1 and OCN, all showed increased expression.

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