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1.
Front Microbiol ; 13: 841157, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35369440

RESUMO

Background/Aims: Intestinal flora, especially Fusobacterium nucleatum (Fn), can affect the development of colorectal cancer (CRC). In this study, we examined the composition of intestinal flora and their metabolites in the tissues, serum and feces of CRC patients. Materials and Methods: CRC tissues, adjacent normal colonic tissues, fecal and serum samples were collected from CRC patients who received surgical treatment between January 2018 and January 2020. Fecal and serum samples were collected from healthy individuals for comparison. In addition, fecal samples were collected from BALB/c female mice. SW480, a human CRC cell line, was utilized for in vitro studies. The experiments involved 1H-NMR-based metabolomics analysis, targeted and untargeted mass spectrometry analysis, and intestinal flora 16S rDNA V4 region sequencing. Results: The abundance of Bacteroides and propionic acid concentration were decreased and that of Lactobacillus and lactic acid concentration were increased in CRC tissues. In addition, the abundances of Ruminococcus, Prevotella, and Sutterell were decreased in CRC patients. The levels of leucine and isoleucine were decreased in the serum and tumor tissues of CRC patients. Aspartate, glutamate and glutathione levels were elevated in the tissues of CRC patients only. The serum glutamine, tyrosine, valine, alanine, and histidine levels were decreased significantly. Lactic acid inhibited and propionic acid promoted apoptosis among SW480 CRC cells. Conclusion: Fn affected the apoptosis of CRC cells and promoted the progression of CRC by affecting the distribution of intestinal flora, which altered the concentrations of metabolites such as lactic acid, propionic acid. Intestinal flora could regulate amino acid metabolism.

2.
BMC Psychiatry ; 22(1): 588, 2022 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-36064380

RESUMO

BACKGROUND: Major depressive disorder (MDD) is a common cause of disability and morbidity, affecting about 10% of the population worldwide. Subclinical depression (SD) can be understood as a precursor of MDD, and therefore provides an MDD risk indicator. The pathogenesis of MDD and SD in humans is still unclear, and the current diagnosis lacks accurate biomarkers and gold standards. METHODS: A total of 40 MDD, 34 SD, and 40 healthy control (HC) participants matched by age, gender, and education were included in this study. Resting-state functional magnetic resonance images (rs-fMRI) were used to analyze the functional connectivity (FC) of the posterior parietal thalamus (PPtha), which includes the lateral habenula, as the region of interest. Analysis of variance with the post hoc t-test test was performed to find significant differences in FC and clarify the variations in FC among the HC, SD, and MDD groups. RESULTS: Increased FC was observed between PPtha and the left inferior temporal gyrus (ITG) for MDD versus SD, and between PPtha and the right ITG for SD versus HC. Conversely, decreased FC was observed between PPtha and the right middle temporal gyrus (MTG) for MDD versus SD and MDD versus HC. The FC between PPtha and the middle frontal gyrus (MFG) in SD was higher than that in MDD and HC. Compared with the HC group, the FC of PPtha-ITG (left and right) increased in both the SD and MDD groups, PPtha-MTG (right) decreased in both the SD and MDD groups and PPtha-MFG (right) increased in the SD group and decreased in the MDD group. CONCLUSION: Through analysis of FC measured by rs-fMRI, the altered FC between PPtha and several brain regions (right and left ITG, right MTG, and right MFG) has been identified in participants with SD and MDD. Different alterations in FC between PPtha and these regions were identified for patients with depression. These findings might provide insights into the potential pathophysiological mechanisms of SD and MDD, especially related to PPtha and the lateral habenula.


Assuntos
Transtorno Depressivo Maior , Habenula , Encéfalo , Mapeamento Encefálico , Depressão , Habenula/diagnóstico por imagem , Humanos , Imageamento por Ressonância Magnética/métodos
3.
Artigo em Inglês | MEDLINE | ID: mdl-36048997

RESUMO

Prior studies suggest that emotional empathy is one of the components of intercultural sensitivity - the affective dimension under the concept of intercultural communication competence. Based on existing theories and findings, this paper reports a randomised parallel longitudinal study investigating the use of virtual reality (VR) exposure to enhance intercultural sensitivity. A total of 80 participants (36 females and 44 males) joined the study and were included in the data analysis. The participants were randomly assigned to the VR group, the video group, and the control group. Their intercultural sensitivity was measured three times: one week before the exposure (T1), right after the exposure (T2), and three weeks after the exposure (T3). The results suggested that (1) the intercultural sensitivity of the VR group was significantly enhanced in both within-subject comparisons and between-subject comparisons, (2) there were no significant differences in intercultural sensitivity between the VR group and the video group at T2, but the VR group retained the enhancement better at T3, and (3) the sense of presence and emotional empathy well predicted the change in intercultural sensitivity of the VR group. The results, together with the participants' feedback and comments, provide new insights into the practice of using VR for intercultural sensitivity training and encourage future research on exploring the contributing factors of the results.

4.
Artigo em Inglês | MEDLINE | ID: mdl-36047047

RESUMO

The purpose of this trial was to evaluate the pharmacokinetics (PK), bioequivalence (BE), and safety of 2 preparations of hydroxychloroquine (200-mg tablet) under fasting and fed conditions. A total of 180 subjects (fasting condition: n = 80; fed condition: n = 100) were randomly enrolled in this randomized, open, single-dose, single-cycle parallel phase Ⅰ clinical study. Under the 2 conditions, the subjects were randomly administered the test (T) or reference (R) tablet, both at a dose of 200 mg (1 tablet). Liquid chromatography-tandem mass spectrometry was used to determine the concentration of hydroxychloroquine in healthy subjects after oral administration of the T or R preparation to evaluate the PK characteristics. In this trial, the T and R preparations of hydroxychloroquine were bioequivalent under both conditions within the range of 80%-125%. No serious adverse events (SAEs) were found in the safety assessments for either condition, and all adverse events (AEs) were mild, except for 2 moderate AEs in the fed condition, indicating good safety.

5.
Brief Bioinform ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094083

RESUMO

Short open reading frames (sORFs) refer to the small nucleic fragments no longer than 303 nt in length that probably encode small peptides. To date, translatable sORFs have been found in both untranslated regions of messenger ribonucleic acids (RNAs; mRNAs) and long non-coding RNAs (lncRNAs), playing vital roles in a myriad of biological processes. As not all sORFs are translated or essentially translatable, it is important to develop a highly accurate computational tool for characterizing the coding potential of sORFs, thereby facilitating discovery of novel functional peptides. In light of this, we designed a series of ensemble models by integrating Efficient-CapsNet and LightGBM, collectively termed csORF-finder, to differentiate the coding sORFs (csORFs) from non-coding sORFs in Homo sapiens, Mus musculus and Drosophila melanogaster, respectively. To improve the performance of csORF-finder, we introduced a novel feature encoding scheme named trinucleotide deviation from expected mean (TDE) and computed all types of in-frame sequence-based features, such as i-framed-3mer, i-framed-CKSNAP and i-framed-TDE. Benchmarking results showed that these features could significantly boost the performance compared to the original 3-mer, CKSNAP and TDE features. Our performance comparisons showed that csORF-finder achieved a superior performance than the state-of-the-art methods for csORF prediction on multi-species and non-ATG initiation independent test datasets. Furthermore, we applied csORF-finder to screen the lncRNA datasets for identifying potential csORFs. The resulting data serve as an important computational repository for further experimental validation. We hope that csORF-finder can be exploited as a powerful platform for high-throughput identification of csORFs and functional characterization of these csORFs encoded peptides.

6.
J Clin Lab Anal ; : e24633, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36085568

RESUMO

BACKGROUND: Kinesin family member 18B (KIF18B) has been regarded as an oncogene that is abnormally overexpressed in some cancers, but its mechanism in esophageal squamous cell carcinoma (ESCC) remains unclear, which is thereby investigated in this study. METHODS: Bioinformatics analysis was performed to analyze the expression of KIF18B in esophageal carcinoma (ESCA). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect KIF18B expression in ESCC cells. After KIF18B overexpression or cell division cycle associated 8 (CDCA8) deficiency, ESCC cells were subjected to determination of qRT-PCR, Western blot, cell counting kit-8 assay, flow cytometry, wound healing, and Transwell assay. The mechanism of KIF18B in the mechanistic target of rapamycin complex 1 (mTORC1) pathway was detected by Western blot. RESULTS: KIF18B was overexpressed in ESCA samples and ESCC cells. Upregulation of KIF18B enhanced the viability, accelerated cell cycle by elevating CDK4 and Cyclin D3 levels as well as promoted the migration and invasion by decreasing E-cadherin level and increasing Vimentin and N-cadherin levels in ESCC cells, which was counteracted by CDCA8 silencing. The expression of CDCA8 in ESCC cells was upregulated by KIF18B overexpression. KIF18B overexpression activated the mTORC1 pathway by upregulating phosphorylated (p)-/p70S6K and p-/mTOR levels in the ESCC cells, which was reversed by CDCA8 silencing. CONCLUSION: KIF18B overexpression promotes the proliferation, migration, and invasion of ESCC cells via CDCA8-mediated mTORC1 signaling pathway in vitro.

7.
Chem Asian J ; 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36074542

RESUMO

Ionic thermoelectric (i-TE) materials have attracted much attention due to their ability to generate ionic Seebeck coefficient of tens of millivolts per Kelvin. In this work, we demonstrate that the ionic thermopower can be enhanced by the introduction of multiple ions. The multi-ionic hydrogel possesses a record thermal-to-electrical energy conversion factor (TtoE factor) of 89.6 mV K-1 and an ionic conductivity of 6.8 mS cm-1, which are both better than single salt contact hydrogel. Subsequently we build a model to explain thermal diffusion of the ions in multi-ionic hydrogels. Finally, the possibility of large-scale integrated applications of multi-ionic hydrogels is demonstrated. By connecting 7 i-TEs hydrogels, we obtained an open-circuit voltage of 1.86 V at ΔT = 3 K. Our work provides a new pathway for the design of i-TEs and low-grade heat harvesting.

8.
Front Plant Sci ; 13: 978531, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119581

RESUMO

Tea (Camellia sinensis L.), as an evergreen plant, needs a humid environment. Water deficit would diminish tea yield and quality. We analyzed the dynamic changes in the metabolite and lipid profiling of tea leaves under various drought conditions and re-watering to determine the metabolic changes in tea leaves responding to drought challenges. In all, 119 metabolites showed substantial alterations in drought-stressed tea plants, including sugars and sugar alcohols, amino acids, and tricarboxylic acid cycle intermediates and lipids. We detected 29 lipids and they were classified into phosphatidylglycerol (PG), phosphatidic acid (PA), sulfoquinovosyl-diacylglycerol (SQDG), phosphatidylcholine (PC), lyso-phosphatidylcholine (LysoPC), and phosphatidylinositol (PI). The levels of sugar, sugar alcohol, and sugar precursors may change as a response to drought stress. Compared with these metabolites, the membrane lipids showed more dynamic changes in tea under drought stresses. Furthermore, metabolic recovery was only partial, with the majority of the examined metabolites exhibiting significantly different levels between samples from re-watered and well-watered tea plants. The findings also showed that comprehensive metabolomic and lipidomic approaches were efficient in elucidating the impacts of drought stress on tea plant metabolism. Our findings are valuable for understanding the mechanisms behind drought tolerance in tea plants from the metabolism perspective and utilizing the compounds to improve the drought tolerance of tea plants.

9.
Front Pharmacol ; 13: 956746, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36120381

RESUMO

Background: Currently, there are many different drugs to improve Alzheimer's disease (AD) from different pathways. As a supplement and alternative medicine, traditional Chinese medicine (TCM) targets multiple pathways which may be different from classical Western medicine, which may be orchestrated with Western medicine to materialize multiplying efficacy in AD patients. Objective: To investigate the therapeutic effect and assess the available preclinical evidence and possible mechanisms of ß-asarone which was extracted from Acorus gramineus Soland (Araceae, AGS) for AD based on rat and mouse animal models. Methods: PubMed, Embase, Scopus, Cochrane Library, BIOSIS Previews, Web of Science, EBSCO, and Google Scholar were searched from inception to 5 May 2022. Rat and mouse experiments assessing the therapeutic effects of ß-asarone for AD were included. Primary outcomes were neuroethology, including escape latency and times of crossing platform. Second outcomes were cell apoptosis, including Bax and Bcl-2. The weighted mean difference (WMD) was generated for continuous variables. The relative outcomes were analyzed with the aid of Get Data Graph Digitizer 2.26 and software STATA version 16.0 MP. Results: For the primary endpoint, compared with the modeling group, ß-asarone significantly decreased the escape latency (WMD = -12.61, 95% CI: -18.66 to -6.57) and increased the times of crossing platform (WMD = 1.50, 95% CI: 0.31-2.70). For the secondary endpoint, ß-asarone remarkably reduced the relative expression of the amyloid precursor protein (APP) (WMD = -2.25, 95% CI: -2.49 to -2.01), decreased the expression of the apoptosis-related protein, associated X protein (Bax) (WMD = -2.40, 95% CI: -3.51 to -1.29), lowered the expression of apoptosis-related protein, B-cell lymphoma-2 (Bcl-2) (WMD = 0.42, 95% CI: 0.38-0.46), and decreased the signal pathway-related proteins, phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) (WMD = -0.70, 95% CI: -0.93 to -0.47) over the control group. Conclusion: ß-asarone spectacularly improved the learning ability and memory in rats and mice, which might be correlated with its potential neuroprotective effect through multiple signaling pathways.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36065917

RESUMO

BACKGROUND: Cancer is associated with metabolic changes from increased cell proliferation and growth. Compared to normal differentiated cells, MM cells use the glycolytic pathway even when adequate oxygen is present triggering "Glutamine addiction." OBJECTIVE: To investigate the single and combined effects of epigallocatechin-3-gallate (EGCG) and telaglenastat, a glutaminase inhibitor, on the proliferation and apoptosis of the multiple myeloma cell line KM3/BTZ. METHODS: KM3/BTZ cells were treated with different concentrations of telaglenastat and EGCG alone or in combination to investigate their effect on proliferation and apoptosis using the CCK8 assay, flow cytometry, and western blotting. The Chou-Talalay combination index analysis was used to explore the effect of telaglenastat combined with EGCG, while the Combination Index (CI) was calculated to analyze whether the combination of the two drugs had a synergistic effect. RESULTS: Telaglenastat and EGCG alone as well as in combination (5 µmol/L telaglenastat + 120 µmol/L EGCG) significantly inhibited the proliferation of KM3/BTZ cells compared to the inhibition effect of the control. Additionally, the combined treatment increased the proportion of KM3/BTZ cells in the G2 phase and decreased the proportion of cells in the G1 phase. The apoptosis rate of EGCG alone and the combined treatment was significantly higher than that of the control group. Bax protein expression was highest in the combined treatment group, whereas Bcl-2 expression was lowest, with the combined treatment group having the highest ratio of Bax/Bcl-2. CONCLUSION: Telaglenastat and EGCG act synergistically to inhibit cell proliferation and promote apoptosis in KM3/BTZ cells, possibly by targeting glutamine metabolism and glycolysis.

11.
Food Sci Biotechnol ; 31(11): 1451-1462, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36060571

RESUMO

Lactobacillus plantarum is an essential probiotic in the human gastrointestinal tract. L. plantarum BF_15, a functional probiotic isolated from the feces of breast-fed infants, has been reported in many in vitro and in vivo studies with strong gastrointestinal adaptability and outstanding anti-oxidative activities. Therefore, the whole genome of L. plantarum BF_15 was sequenced. Several genes, encoding the gastrointestinal adaptability-related proteins, were identified, including genes related to gastrointestinal environment-induced stress resistance, adhesive performance, and ability to transport and metabolize resistant starch and oligosaccharides. Genes related to alleviating oxidative stress were also found. Further functional verification was carried out by RT-qPCR on the 10 and 12 key adhesion and antioxidant genes. Overall, this study might provide a critical basis for L. plantarum BF_15 as a potential candidate for probiotics. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-022-01132-w.

12.
Heliyon ; 8(9): e10343, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36097478

RESUMO

In this work, we determined an approximate eigen solutions of Modified multi-parameter exponential potential using supersymmetric quantum mechanics approach (SUSY) with improved Greene-Aldrich approximation to the centrifugal term. The energy equation and its corresponding normalised radial wave function were fully obtained. The proposed potential reduces to other useful potentials like Rosen-Morse, Hellmann, Yukawa and Coulomb potential as special cases. The thermodynamic properties like the vibrational mean energy ( U ß , V ), Vibrational heat capacity ( C ß , V ), vibrational entropy ( S ß , V ) and vibrational free energy ( F ß , V ) of the interacting potential were studied via partition function ( Z ß , V ) obtained from the resulting energy equation. This study was applied to three diatomic molecules: Chromium hydride (CrH), Titanium Hydride (TiH) and Thiocynate (ScN). To ascertain the high degree of our analytical mathematical accuracy, we compared the results of special cases with an existing results. These were found to be in excellent agreement with the existing results.

13.
Int J Mol Sci ; 23(17)2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36077077

RESUMO

Plant C2-domain abscisic acid-related (CAR) protein family plays an important role in plant growth, abiotic stress responses, and defense regulation. In this study, we cloned the IbCAR1 by homologous cloning method from the transcriptomic data of Xuzishu8, which is a sweet potato cultivar with dark-purple flesh. This gene was expressed in all tissues of sweet potato, with the highest expression level in leaf tissue, and it could be induced by NaCl and ABA. Subcellular localization analyses indicated that IbCAR1 was localized in the nucleus and plasma membrane. The PI staining experiment revealed the distinctive root cell membrane integrity of overexpressed transgenic lines upon salt stress. Salt stress significantly increased the contents of proline, ABA, and the activity of superoxide dismutase (SOD), whereas the content of malondialdehyde (MDA) was decreased in overexpressed lines. On the contrary, RNA interference plants showed sensitivity to salt stress. Overexpression of IbCAR1 in sweet potatoes could improve the salt tolerance of plants, while the RNAi of IbCAR1 significantly increased sensitivity to salt stress in sweet potatoes. Meanwhile, the genes involved in ABA biosynthesis, stress response, and reactive oxygen species (ROS)-scavenging system were upregulated in overexpressed lines under salt stress. Taken together, these results demonstrated that IbCAR1 plays a positive role in salt tolerance by relying on the ABA signal transduction pathway, activating the ROS-scavenging system in sweet potatoes.


Assuntos
Ipomoea batatas , Ácido Abscísico/metabolismo , Regulação da Expressão Gênica de Plantas , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Tolerância ao Sal/genética , Estresse Fisiológico/genética
14.
BMC Oral Health ; 22(1): 395, 2022 09 12.
Artigo em Inglês | MEDLINE | ID: mdl-36096796

RESUMO

OBJECTIVES: Morphological study is a common approach in the field of anterior disc displacement (ADD) pathology; however, analysis based on three-dimensional reconstructive imaging has not been investigated. This study investigated the association between ADD and the status of the mandibular condyle and articular fossa. METHODS: Thirty-four patients were divided into three groups: normal articular disc position (NADP), anterior disc displacement with reduction (ADDwR), and anterior disc displacement without reduction (ADDwoR). Multiple grouped comparisons of three different disc statuses were performed by Kruskal-Wallis H test and variance analysis respectively. Receiver-operating characteristic curve was plotted to assess the diagnostic efficacy of the morphological parameters. Multivariate logistic regression analysis was used to investigate the interfering factors of ADD. RESULTS: The condylar volume (CV) and condylar superficial area (CSA) in the NADP, ADDwR, and ADDwoR groups exhibited obvious changes (P < 0.05). Both CV and superior joint space (SJS) presented a good diagnostic accuracy for NADP-ADDwoR [area under the curve (AUC)CV = 0.813; AUCSJS = 0.855)], and ADDwR-ADDwoR (AUCCV = 0.858; AUCSJS = 0.801). CSA presented a good diagnostic accuracy for ADDwR-ADDwoR (AUC = 0.813). A multivariate logistic ordinal regression model showed that the CV [odds ratio (OR) = 1.011; regression coefficient (RC) = 0.011, P = 0.018], SJS (OR, 8.817; RC = 2.177; P < 0.001), and medial joint space (MJS) (OR, 1.492; RC = 0.400; P = 0.047) had a significantly impact on the groups. CONCLUSION: CV, CSA, SJS, and MJS were significantly associated with the different disc status, and the condyle in ADD exhibited 3-dimensionally altered dimensions. They could be considered as promising biometric markers to assess the ADD.


Assuntos
Transtornos da Articulação Temporomandibular , Humanos , Côndilo Mandibular/diagnóstico por imagem , Côndilo Mandibular/patologia , NADP , Articulação Temporomandibular , Transtornos da Articulação Temporomandibular/patologia
15.
Arch Comput Methods Eng ; : 1-35, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36091717

RESUMO

With the acceleration of urbanization and living standards, microorganisms play an increasingly important role in industrial production, bio-technique, and food safety testing. Microorganism biovolume measurements are one of the essential parts of microbial analysis. However, traditional manual measurement methods are time-consuming and challenging to measure the characteristics precisely. With the development of digital image processing techniques, the characteristics of the microbial population can be detected and quantified. The applications of the microorganism biovolume measurement method have developed since the 1980s. More than 62 articles are reviewed in this study, and the articles are grouped by digital image analysis methods with time. This study has high research significance and application value, which can be referred to as microbial researchers to comprehensively understand microorganism biovolume measurements using digital image analysis methods and potential applications.

16.
Biomed Environ Sci ; 35(8): 681-687, 2022 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-36127780

RESUMO

Objective: This study is to obtain precise data on iron physiological requirements in Chinese children using single stable isotope tracer technique. Methods: Thirty boys (10.6 ± 0.2 years) and 27 girls (10.4 ± 0.2 years) were received oral 6 mg 57Fe each day for 5 consecutive days. Venous blood samples were subsequently drawn to examine the change of total iron concentration and 57Fe abundance at day 0, 14, 28, 60, 90, 180, 360, 450, 540, 630, 720. The iron physiological requirement was calculated by iron loss combined with iron circulation rate once 57Fe abundance stabilized in human body. Results: The iron physiological requirement was significantly lower in boys than those values in girls (16.88 ± 7.12 vs. 18.40 ± 8.81 µg/kg per day, P < 0.05). Correspondingly, the values were calculated as 722.46 ± 8.43 µg/day for boys and 708.40 ± 7.55 µg/day for girls, respectively. Considering nearly 10% iron absorption rate, the estimated average iron physiological requirement was 6.0 mg/day in boys and 6.2 mg/day in girls. Conclusion: This study indicate that iron physiological requirement could require more daily iron intake in girls as compare with the values in boys having the same body weight. These findings would be facilitate to the new revised dietary reference intakes.

17.
Manuf Lett ; 33: 835-842, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36128336

RESUMO

In a world where demand and supply can change instantaneously as demonstrated by the recent coronavirus pandemic, it is very important for our production lines to be able to handle abrupt changes very effectively. Flexible manufacturing systems (FMS) are designed to be able to ramp production up and down quickly to make this possible. In this paper, we propose a novel data-enabled method of modelling an FMS using mobile multi-skilled robots and evaluate its dynamic performance. We use permanent production loss as the performance metric and derive expressions for its evaluation and attribution and validate them using two case studies.

18.
Artigo em Inglês | MEDLINE | ID: mdl-36123300

RESUMO

Low photoluminescence quantum difference rate and weak targeting ability make the practical application of NIR-II nanoparticles challenging. Here, NIR-II chiral rare-earth doped Ag 2 Se nanoparticles (defined as R- or S-Ag 2 Se NPs) were fabricated, exhibiting circular dichroism signal peak at 850 nm and fluorescence emission peak at 1550 nm, with 145.7-fold enhanced intensity compared to the reported Ag 2 Se NPs. Compared with S-Ag 2 Se NPs, a significant improvement in imaging efficiency of R-Ag 2 Se NPs was observed in living cells, which was attributed to a higher cellular uptake rate and 927.7-fold higher affinity. Furthermore, R-Ag 2 Se NPs reached at the tumor 2-fold faster than S-Ag 2 Se NPs in vivo . We discover that chirality leads to differences in the affinity of chiral Ag 2 Se NPs for 4T1 cell surface CD44, further causing the differences of in vivo imaging efficiency. These results reveal that chiral Ag 2 Se NPs have high photoluminescence intensity and high in vivo imaging efficiency reflecting wide applications in biomedical diagnosis.

19.
Mol Genet Genomic Med ; : e1965, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36124717

RESUMO

BACKGROUND: MN1 C-terminal truncation (MCTT) is a rare syndrome; only 27 cases have been reported. We report the first case of an 8-year-old girl with MCTT syndrome complicated with moderate obstructive sleep apnea (OSA). METHODS: MCTT syndrome was diagnosed by whole-exome sequencing (WES) and validated by Sanger sequencing. The patient received 2 years of treatment with continuous positive airway pressure (CPAP) to relieve sleep apnea and hypoxia, and a reverse sector fan-shaped expander for maxillary expansion. RESULTS: WES revealed a de novo MN1 variant, c.3760C>T (p.[Q1254*]). An arachnoid cyst was found in the right occipital brain. The patient presented mild symptoms of classic MCTT syndrome. The patient did not experience hearing loss and only mild intellectual disability. Radiological examinations showed cleft secondary palate, narrow upper arch, narrow upper airway, and mandibular skeletal retrusion. Polysomnography indicated moderate OSA, with an apnea/hypopnea index of 6.8, which decreased to 1 after CPAP during the night. Two-year maxillary expansion widened the upper arch, and the cleft secondary palate became visible. The mandible moved forward spontaneously, resulting in the improvement of profile and upper airway widening. General physical conditions, such as motor delay, muscle weakness, and developmental delay, were significantly improved two years later. CONCLUSION: In conclusion, we discovered a MN1 variant [NM_002430.2: c.3760C>T, p.Q1254*] that causes mild MCTT symptoms compared to other MN1 variants. For patients with MCTT complicated with OSA, multidisciplinary combination therapy can improve maxillofacial development, widen the upper airway and relieve sleep apnea, improving the general physical condition.

20.
Clin Lab ; 68(9)2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36125146

RESUMO

BACKGROUND: Deficiency of vitamin B(12) or folate causes megaloblastic anemia (MA). The disease presents with pancytopenia due to the excessive cellular apoptosis of hematopoietic progenitor. MA is characterized by the presence of high mean corpuscular volume in the blood routine test and hyperlobulation nuclei of the granulocytes in the peripheral blood smears, and megaloblasts in the bone marrow. METHODS: We report a rare case, in which megaloblastic anemia was masked by an unrecognized hemoglobinopathy and presented with normocytic anemia and atypical morphological features of bone marrow. RESULTS: The patient was finally diagnosed with coexistence of MA and a-thalassemia minor due to determination of folate deficiency and genetic mutation for a-thalassemia. CONCLUSIONS: The case focuses on the contribution of the peripheral circulating blood smear examination in the diagnosis of anemia.

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