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1.
J Cell Physiol ; 235(1): 380-393, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31232476

RESUMO

Atherosclerosis (AS), a chronic disorder of large arteries, is the underlying pathological process of heart disease and stroke. Former researchers have found that microRNAs (miRs) are involved in the several key processes of AS. Apolipoprotein E knockout (ApoE-/- ) mice fed a high-fat-diet (HFD) to establish AS model. The expression of miR-103 was characterized in the mice model. The effects of miR-103 on inflammation and endoplasmic reticulum stress (ERS) were analyzed when the expression of miR-103 was inhibited in ApoE -/- mice fed an HFD and human aortic endothelial cells (HAECs) exposed to oxidized low-density lipoprotein (ox-LDL). The relationship between miR-103 and phosphatase and tensin homolog (PTEN) was identified by luciferase activity detection and real-time quantitative polymerase chain reaction (RT-qPCR). Gain- and loss-function approaches were further applied for investigating the regulatory effects of miR-103 and PTEN on ERS. Role of MAPK signaling was then analyzed using PD98059 to block this pathway. miR-103 was highly expressed in the ApoEApoE -/- mice fed an HFD. Downregulation of miR-103 suppressed inflammation and ERS in endothelial cells isolated from ApoE -/- mice fed a HFD and ox-LDL-exposed HAECs. In addition, miR-103 can target PTEN and downregulate its expression. Overexpression of PTEN reversed the miR-103-induced activation of MAPK signaling. Moreover, PTEN upregulation or MAPK signaling inhibition ease miR-103-induced inflammation and ERS in vivo and in vitro. Thus, miR-103 depletion restrains the progression of AS through blocking PTEN-mediated MAPK signaling.

2.
Nanotechnology ; 31(3): 035403, 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31557753

RESUMO

The development of low-cost electrocatalysts with high performance is important to provide sustainable hydrogen energy. In this work, via one-step sulfuration of [Formula: see text] intercalated NiFe-layered double hydroxide (abbr. NiFe-MoO4-LDH), hierarchical microspheres are assembled by intersecting nanoplates (15-30 nm in thickness) which are then decorated with MoS2 and (NiFe)S x nanoparticles (∼25 nm in size). The NiFe-MoO4-LDH is synthesized beforehand by a one-pot hydrothermal reaction. Under sulfuration at 300 °C, 400 °C and 600 °C, the NiFe-MoO4-LDH transforms into multi-metal sulfides of NiFeMoS-T (T is applied temperature). During sulfuration, the confinement effect of LDH limits the growth of metal sulfides, causing formation of nanoparticles of MoS2 and (NiFe)S x to expose more catalytic active sites. In an alkaline medium, NiFeMoS-400 depicts superior performance for hydrogen evolution reaction (HER), giving an overpotential of 210 mV at 10 mA cm-2. A Tafel slope of 88 mV dec-1 indicates a mixed Volmer-Heyrovsky rate-determining step. The electrode also maintains long-term electrochemical durability during 15 h electrolysis at 25 mA cm-2. The NiFe-MoO4-LDH precursor owns three metal elements (Ni, Fe and Mo), which ensure the formation of polymetallic sulfides, and maximum utilization of the LDH layer and interlayer metals contributes to the optimal electrocatalytic activity. The NiFeMoS nanoassembly is a potential low-cost and high-efficiency electrocatalyst.

3.
Ecotoxicol Environ Saf ; 187: 109821, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31677572

RESUMO

In this study, a porous framework MOF-74(Zn) (Zn2 (DHBDC)(DMF)(H2O)2, H4dondc = 1, 5-dioxido-2, 6-naphthalenedicarboxylic acid) with open metal sites was successful synthesized. MOF-74(Zn) as a template was grafted on the open metal sites with ethylenediamine (en) named MOF-74(Zn)-en to develop a highly selective and sensitive fluorescence detector for rapid determination of tetrabromobisphenol A (TBBPA). The obtained MOF-74(Zn)-en was well characterized by Fourier Transform Infrared (FT-IR), Scanning Electron Microscopy (SEM) and showed ideal properties of photoluminescence. The fluorescence enhancement showed a good linear relationship with the concentrations of TBBPA in the range of 50-400 µg/L, and its limit of detection could reach to 0.75 µg/L. Furthermore, the possible sensing mechanism of the fluorescence enhancement could be attributed to Förster resonance energy transfer (FRET). The results will provide a convenient and quick method for detection of TBBPA. To the best of my knowledge, this is the first case to detect TBBPA by fluorescence enhancement with MOF derivatives.

4.
J Hazard Mater ; 382: 121018, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31446354

RESUMO

The broad spectrum detection of veterinary drugs is very important for rapid and large-scale safe screen of animal-derived foods. Metal-organic frameworks (MOFs), as a kind of emerged functional porous materials are quite promising in the chemical sensing and molecular detection. In this work, we report the high-performance broad spectrum detection of 15 commonly-used veterinary drugs through the fluorescence quenching in a newly-designed chemically stable Al-based MOF, Al3(µ3-O)(OH)(H2O)2(PPTTA)3/2 (BUT-22). To the best of our knowledge, this is the first systematic investigation for the application of MOFs in the detection/sensing of veterinary drugs through fluorescence quenching method. The quenching efficiencies of the tested veterinary drugs on BUT-22 are all beyond 82%, and the limits of detection (LOD) are low at parts per billion (ppb) levels. Interestingly, BUT-22 also enables the selective detection of nicarbazin (NIC) through the clearly-observed red shift of its maximum fluorescence emission wavelength. Moreover, the fluorescence quenching mechanism was explored with the help of theoretical calculations. Our work indicates that MOFs are favorable materials for the detection of veterinary drugs, being potentially useful in monitoring drug residues of animal-derived foods.

5.
Cell Signal ; 65: 109431, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31654721

RESUMO

The fusion oncogene, promyelocytic leukemia (PML)-retinoic acid receptor-α (RARα), is crucial for acute promyelocytic leukemia (APL) pathogenesis. Previous studies have reported that PML-RARα is cleaved by neutrophil elastase (NE), an early myeloid-specific serine protease, leading to translocation of the nuclear localization signal (NLS) of the PML protein to the N-terminal of RARα. This study was designed to evaluate the value of NLS-RARα in the early diagnosis of APL. To investigate the potential functional role of NLS-RARα in leukemogenesis, HL-60 and U937 cell lines were transfected with NLS-RARα lentivirus and negative control (LVNC). The results showed that the induced expression of NLS-RARα down-regulated expressions of CD11b, CD11c, and CD14 compared to the LVNC group induced by 1α, 25-dihydroxyvitamin D3(1,25(OH)2D3). This suggested that NLS-RARα overexpression inhibited granulocytic and monocytic differentiation of myeloid leukemia cells. In addition, Wright-Giemsa staining, flow cytometry, respiratory burst assay, and NBT reduction assay all confirmed the importance of NLS-RARα in differentiation. The mechanistic investigations revealed that induced NLS-RARα expression inhibited 1,25(OH)2D3-induced granulocytic differentiation by regulating the cell cycle regulators p19INK4D, p21WAF1/CIP1, cyclinD1, cyclin E1, and pRB. Furthermore, the cleaved protein NLS-RARα enhanced the oncogenicity of U937 cells in NOD/SCID mice. These findings collectively demonstrated that NLS-RARα blocked granulocytic and monocytic differentiation of myeloid leukemia cells by inhibiting the downstream targets of the RARα signal pathway and the cell cycle. This may provide a promising new target and method for diagnosing and treating APL.

6.
Per Med ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31686591

RESUMO

Aim: The aim of the present study was to examine the predictive value of lipoprotein(a) (Lp[a]) levels for coronary collateral circulation (CCC) in patients with acute myocardial infarction (AMI). Method & methods: A total of 409 consecutive patients with AMI were enrolled for this study. Patients were divided into two groups according to rentrop grades assessed by coronary angiography: bad (n = 277) and good CCC group (n = 132). Result: Patients with bad CCC had a higher level of Lp(a) than that with good CCC (median Lp[a] 219.1 vs 122.0 mg/l). The area under the receiver-operating characteristic curves of Lp(a) in predicting bad CCC was 0.647 (95% CI: 0.592-0.702) with the cut-off value of 199.0 mg/l. Conclusion: Our data firstly suggested that Lp(a) might be a useful marker for CCC after AMI.

7.
Urology ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31715271

RESUMO

OBJECTIVE: To assess the risk of biochemical recurrence (BCR) in small low-grade prostate tumours following radical prostatectomy (RP), which are defined as clinically insignificant based on the existing criteria developed by Stamey and Epstein. MATERIALS AND METHODS: We identified 3784 men who underwent RP in Western Australia from September 1998 to March 2019. These patients had a Gleason sum (GS) of ≤ 6 or 3+4, prostate confined and negative margins. Pathological data analysis was performed using logistic regression modelling. RESULTS: Median follow up was 96.8 months. BCR occurred in 110 men (2.91%). There was no statistical difference in the rates of failure for patients with a tumour volume <0.5ml vs 0.5-2.0ml when comparing (i) those with Gleason 6, or (ii) those with Gleason 3+4. Furthermore, there was no statistical difference in rates of failure when comparing patients with a tumour volume of ≤2ml vs >2ml when the percentage of Gleason pattern 4 was ≤20%. However, once the percentage of Gleason pattern 4 increased to 30%, there was a significant increase in BCR in the larger tumours (> 2ml). CONCLUSION: This study did not support either Stamey's or Epstein's criteria of insignificant cancer based on volumes of less than 0.5ml, GS <7 and confined margin negative disease. No risk free cancer was identified, as all groups demonstrated some risk of BCR. This study redefines the entity of insignificant cancer as rather "low risk" cancer and expands its scope to include smaller tumours with minor Gleason pattern 4 components.

8.
Int J Mol Sci ; 20(22)2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31717686

RESUMO

Plant genome sequences are presently deciphered at a staggering speed, due to the rapid advancement of high-throughput sequencing technologies. However, functional genomics significantly lag behind due to technical obstacles related to functional redundancy and mutant lethality. Artificial microRNA (amiRNA) technology is a specific, reversible, and multiplex gene silencing tool that has been frequently used in generating constitutive or conditional mutants for gene functional interrogation. The routine approach to construct amiRNA precursors involves multiple polymerase chain reactions (PCRs) that can increase both time and labor expenses, as well as the chance to introduce sequence errors. Here, we report a simplified method to clone and express amiRNAs in Arabidopsis and rice based on the engineered Arabidopsis miR319a or rice miR528 precursor, which harbor restriction sites to facilitate one-step cloning of a single PCR product. Stem-loop reverse-transcriptase quantitative PCR (RT-qPCR) and functional assays validated that amiRNAs can be accurately processed from these modified precursors and work efficiently in plant protoplasts. In addition, Arabidopsis transgenic plants overexpressing the modified miR319a precursor or its derived amiRNA could exhibit strong gene silencing phenotypes, as expected. The simplified amiRNA cloning strategy will be broadly useful for functional genomic studies in Arabidopsis and rice, and maybe other dicotyledon and monocotyledon species as well.

9.
Respir Care ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31719191

RESUMO

BACKGROUND: We sought to investigate the effects of home-based breathing exercises on pulmonary function, respiratory muscle strength, exercise capacity, dyspnea, and health-related quality of life in patients with COPD. METHODS: All randomized, controlled trials involving the use of home-based breathing exercises as an intervention in patients with COPD were searched on PubMed, Embase, Web of Science, EBSCO, CNKI, and Wangfang Data databases from January 1, 2008, to December 31, 2018. Two researchers independently extracted data and assessed the quality of the literature that met the inclusion criteria. RESULTS: A total of 13 studies were included, with a total of 998 subjects. The intervention methods consisted of diaphragmatic breathing, yoga breathing, breathing gymnastics, and singing. Meta-analysis showed that, compared with the control group, home-based breathing exercises had significant effects on the percent of predicted FEV1 (mean difference = 3.26, 95% CI 0.52-5.99, P = .02), FEV1/FVC (mean difference = 2.84, 95% CI 1.04-4.64, P = .002), maximum inspiratory pressures (mean difference = 20.20, 95% CI 11.78-28.61, P < .001), maximum expiratory pressures (mean difference = 26.35, 95% CI 12.64 to 40.06, P < .001), 6-min walk distance (mean difference = 36.97, 95% CI 25.06-48.89, P < .001), the modified Medical Research Council dyspnea scale (mean difference= -0.80, 95% CI -1.06 to -0.55, P < .001), and the St George Respiratory Questionnaire (mean difference= -8.62, 95% CI -13.09 to -4.16, P < .001). CONCLUSIONS: As an alternative method of home-based pulmonary rehabilitation program, breathing exercises can improve pulmonary function, respiratory muscle strength, exercise capacity, dyspnea, health-related quality of life in patients with COPD.

10.
J Mater Chem B ; 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31720683

RESUMO

Transplantation of tissue-engineered neural scaffolds bears great potential for reconstructing neural circuits after spinal cord injury (SCI). In this study, a 3D porous silk fibrous scaffold (3D-SF) with biomimetic interconnected micro- to nanofibrous structure and good biocompatibility is fabricated. Then, a small-molecule combination CFLSSVY (CHIR99021, Forskolin, LDN193189, SB431542, SP600125, VPA, and Y27632) that efficiently reprograms rat dermal fibroblasts into neurons is screened, and these chemically induced neurons (CiNs) are shown to readily communicate on the 3D-SF and form neural scaffolds. After transplantation of these silk-based neural scaffolds into the stumps of transected spinal cords in rats, the damaged tissue is repaired significantly, as indicated by the reduced cavity areas, decreased GFAP expression, and improved axonal regeneration and myelination in the injury site. Moreover, the hindlimb movement and motor-nerve conductivity are greatly improved as indicated by the elevated BBB score, the alternate movement of two hindlimbs during the 45° inclined grid test, and the shortened latency and enhanced amplitude in cMEP detection. Together, these results demonstrate that transplantation of neural scaffolds consisting of 3D-SF and dermal fibroblast-reprogrammed neurons leads to significant nerve regeneration and functional recovery, providing a promising therapeutic strategy for SCI.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31720785

RESUMO

Activation of complement system in central nervous system (CNS) of the patients suffering from prion diseases or animal models infected with prion agents experimentally is reported repeatedly, but which pathways are involved in the complement system during prion infection is not well documented. Here, we evaluated the level of complement factor B (CFB), which is the key factor that triggers alterative pathway (AP) of complement in the brain tissues of scrapie-infected mice with various methodologies. We found that the levels of mRNA and protein of CFB significantly increased in the brain tissues of scrapie-infected mice. Morphologically, the increased CFB-specific signal overlapped with the elevated C3 signal in brain sections of scrapie-infected mice, meanwhile overlapped with damaged neurons and activated microglia, but not with the proliferative astrocytes. Additionally, the level of complement factor P (CFP), the key positive regulator of AP, also increased remarkably in the brain tissues of infected mice. The transcriptional levels of CD55 and CD46, two negative regulators of AP, decreased without significance in brain tissues of scrapie-infected mice at the terminal stage. However, the mRNA and protein levels of CFH, another negative regulator of AP, increased. Through the dynamic analyses of the expressions of CFB, CFP, and CFH in brain sections of 139A-infected mice, which were collected at different time-points during incubation period, illustrated time-dependent increase levels of each factor during the incubation period of scrapie infection. Taken together, our data here demonstrate that the AP of complement cascade is activated in the CNS microenvironment during prion infection.

12.
Atherosclerosis ; 291: 27-33, 2019 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-31683090

RESUMO

BACKGROUND AND AIMS: Lipoprotein(a) [Lp(a)] has been considered as a causal risk factor for cardiovascular disease (CVD) in the general population and levels vary in different ethnicities. However, no systemic analysis is currently available regarding the relation of plasma Lp(a) levels to cardiovascular events (CVEs) in Chinese patients with heterozygous familial hypercholesterolemia (HeFH). METHODS: Three hundred and ninety-three patients with HeFH undergoing Lp(a) measurement at baseline were consecutively enrolled and followed prospectively for an average of 36.5 months. Lp(a) levels were determined using an immunoturbidimetry assay. Cox regression analysis with adjusted hazard ratios (HRs) and Kaplan-Meier analysis were used to evaluate the prognostic value of Lp(a) on CVEs. RESULTS: Thirty-five events occurred during follow-up. Lp(a) was significantly higher in patients with CVEs (53.3 mg/dL versus 31.7 mg/dL, p < 0.001). In Kaplan-Meier analysis, patients with upper tertile of Lp(a) had a significant lower event-free survival (p = 0.004). After adjusting for confounding risk factors, per log unit increase in baseline Lp(a) was independently associated with CVEs [HR: 2.03(1.28-3.21), p = 0.002]. HRs remained unchanged after accounting for hard endpoints and did not vary too much in several relevant subgroups. Adding Lp(a) to the Cox model led to a significant improvement in C-statistic, net reclassification and integrated discrimination. Moreover, HR for upper versus lower tertile of change in Lp(a) was 2.68 (1.11-6.48) for CVEs after one year. CONCLUSIONS: Both baseline and on-statin treatment Lp(a) levels were associated with an increased risk of CVEs in patients with HeFH, suggesting that Lp(a) measurement might clinically help further risk stratification of FH patients.

13.
Per Med ; 16(6): 467-478, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31691639

RESUMO

Aim: To investigated the potential differences between probable and definite heterozygous familial hypercholesterolemia (HeFH) patients diagnosed by Dutch Lipid Clinic Network criteria. Methods: Clinical characteristics, lipid profile, severity of coronary artery stenosis and gene mutations were compared. Kaplan-Meier curve was performed to evaluate the cardiovascular events. Results: Overall, 325 participants were included and divided into two groups: probable (n = 233) and definite HeFH (n = 92). Definite HeFH patients had higher low-density lipoprotein cholesterol (LDL-C), oxidized-LDL and proprotein convertase subtilisin/kexin 9 levels, and higher prevalence of tendon xanthomas. The incidence of genetic mutations was statistically higher in definite HeFH than probable HeFH patients. The coronary stenosis calculated by Gensini score was statistically severer in definite HeFH patients. The best LDL-C threshold for predicting mutations was 5.14 mmol/l. Definite HeFH had lower event-free survival rates. Conclusion: Definite HeFH patients had higher severity of phenotype and genotype, and higher risk of cardiovascular events.

15.
Res Vet Sci ; 127: 65-75, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31678455

RESUMO

This study was planned to investigate TD (Tibial dyschondroplasia) on the potential MAPK signaling pathway and angiogenesis related genes. Forty-eight broilers were allotted into control (C) and treatment (T) groups of 2, 6 and 15 days as C1, C2, C3, T1, T2 and T3. The histopathology results revealed that tibiotarsus bone of chickens had more lesions on day 6 (T2 group). The chondrocytes were disordered, and the size, shape and proliferation were affected. Transcriptome results revealed that differentially expressed genes (DEGs) identified were 63, 1026, 623, 130, 141 and 146 in C1 (2 days control vs 6 days control); C2 (2 days control vs 15 days control); C3 (6 days control vs 15 days control); T1 (2 days treatment vs 6 days treatment); T2 (2 days treatment vs 15 days treatment) and T3 (6 days treatment vs 15 days treatment) groups respectively. Whereas, 10 angiogenesis related-genes RHOC, MEIS2, BAIAP2, TGFBI, KLF2, CYR61, PTPN11, PLXNC1, HSPH1 and NRP2 were downregulated on day 6 in the treatment group. The pathway which was found enriched in the control and treatment groups was MAPK signaling pathway. Therefore selected 10 MAPK signaling pathway-related genes RAC2, MAP3K1, PRKCB, FLNB, IL1R1, PTPN7, RPS6KA, MAP3K6, GNA12 and HSPA8 which were found significantly downregulated in the treatment group on day 6. It is concluded that angiogenesis and MAPK signaling pathway related genes has an essential role in TD, as those top screened genes found downregulated in the thiram fed chickens when TD observed severed on day 6.

16.
Anal Chem ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31682106

RESUMO

Surface enhanced Raman spectroscopy (SERS) is an ultrasensitive label-free analytical technique that can provide unique chemical and structural fingerprint information. However, gaining reliable quantitative analysis with SERS remains a huge challenge because of poor reproducibility and the instability of nanostructured SERS active surfaces. Herein, an effective strategy of coating Au nanoparticles (NPs) with ultrathin and uniform Prussian blue (PB) shell (Au@PB NPs) was developed for quantitative detection of dopamine (DA) concentrations in blood serum and crystal violet (CV) contaminants in lake water. The only intense PB Raman signal at 2155 cm-1 served as an ideal and interference-free internal standard (IS) for correcting fluctuations in the Raman intensities of analytes. Also, the stability of Au@PB NPs was investigated, exhibiting good functionality in strong acid solutions and thermal stability at 100 °C. This work demonstrates a convenient and fast quantitative SERS technique for detecting analyte concentrations in complex systems and has a great number of potential applications for use in analytical chemistry.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31722527

RESUMO

Creation of sustainable, cost-effective and scalable absorbents with ideal absorption properties is a worldwide challenge because many high-performance absorbents are still restricted in laboratory scope due to several critical defects (like complex and eco-unfriendly synthesis process, high cost, and difficulty in large-scale production). Herein, a facile and scalable top-to-bottom design is proposed to create a kind of novel anisotropic carbon aerogels with low tortuosity of stacked laminated structure, derived from the hierarchical cellular channels of balsa wood. In virtue of this unique structure and favorable oleophilicity, a fast passive capillary absorption with low flow resistance is achieved (as demonstrated by the theoretical modeling). As a result, the anisotropic carbon aerogels have quite sensitive selectivity to separate organic pollutants from water, broad-spectrum and high absorption capacity for different organic liquids (13277‒31597 mg g‒1), and superior recyclability (98.7% absorption capacity retention after 5 cycles). Combining these outstanding performances with cheap preparation strategy as well as good environmental friendliness, this work provides a kind of potential scalable materials for efficient reusable absorption and separation of organic leakages.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31724298

RESUMO

This contribution reports for the first time about the use of carbon dots (CDs) as sensitizer to initiate and activate free radical and controlled radical polymerization based on an ATRP protocol with blue LEDs, respectively. Consideration of diverse heteroatom-doped CDs indicated well-functioning of N-doped CDs as effective photocatalyst and photosensitizer in combination with LEDs emitting either at 405 nm or 470 nm.  Initiation of free radical polymerization occurred by combining the CDs with an iodonium or sulfonium salt in tri(propylene glycol) diacrylate.  Polymerization of methyl methacrylate (MMA) by photo-induced ATRP was achieved with CDs and ethyl a-bromophenylacetate using Cu(II) as catalyst in the ppm range.  The polymers obtained showed temporal control, narrower dispersity â‰²1.5, and chain-end fidelity. The first order obtained from kinetic slope and ON/OFF experiments additionally approved constant concentration of polymer radicals.  No remarkable cytotoxic activity was observed of the CDs underlining their biocompatibility.

19.
Food Funct ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31696186

RESUMO

Flaxseed oil (FO), enriched in n-3 polyunsaturated fatty acids (PUFAs), is an important oil source for intestinal development and health. We aimed to study the different effects of FO versus soybean oil (SO) on growth, intestinal health and immune function of neonates with intrauterine growth retardation (IUGR) using a weaned piglet model. Forty pairs of male IUGR and normal birth weight piglets, weaned at 21 ± 1 d, were fed diets containing either 4% FO or SO for 3 weeks consecutively. Growth performance, nutrient digestibility and intestinal function parameters, immunology and microbiota composition were determined. IUGR led to a poor growth rate, nutrient digestibility and abnormal immunology variables, whereas feeding FO diet improved systemic and gut immunity, as indicated by increased plasma concentration of immunoglobulin G and decreased CD3+CD8+ T lymphocytes, and down-regulated intestinal expression of genes (MyD88, NF-κB, TNF-α, IL-10). Although IUGR tended to decrease villous height, feeding FO diet tended to increase the villi-crypt ratio and up-regulated expressions of tight junction genes (Claudin-1 and ZO-1), together with increased mucosa contents of n-3 PUFAs and a lower Σn-6/Σn-3 ratio. Besides, FO diet decreased the abundance of pathogenic bacteria Spirochaetes, and increased phylum Actinobacteria, and genera Blautia and Bifidobacterium in colonic digesta. Our findings indicate that IUGR impairs growth rate, nutrient digestibility, and partly immunology variables, whereas feeding FO-supplemented diet could improve intestinal function and immunity of both IUGR and NBW pigs, associated with the altered gut microbiome and mucosal fatty acid profile.

20.
Hemoglobin ; : 1-3, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690135

RESUMO

A female of Chinese origin carried the codon 43 (G>T) (HBB: c.130G > T) and codons 71/72 (+A) (HBB: c.216_217insA) mutations of the ß-globin gene in cis, identified during prenatal thalassemia screening. The double in cis mutations were inherited from her mother. Both of the two carriers behave as a traditional heterozygote for ß-thalassemia (ß-thal) with microcytosis and a high Hb A2 level. This case report indicates that the possibility of multiple mutations in cis in a fetus with thalassemia trait has to be considered in a prenatal screening program.

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