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1.
J Ethnopharmacol ; 246: 112240, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31526861

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: You-Gui-Yin (YGY) is a famous Chinese traditional medicine compound that has been used to treat renal function diseases for more than 300 years. It is recorded in Jing Yue Quanshu, which was written by a famous medical scientist named Jiebing Zhang in the Ming Dynasty. AIM OF THE STUDY: Reproductive dysfunction is one of the most serious complications of chronic kidney disease (CKD). The aim of this study was to observe the effect of You-Gui-Yin (YGY) on reproductive dysfunction of male rats with adenine-induced CKD and to determine if any effects occurred via regulation of the HIF1α-STAT5 pathway. MATERIALS AND METHODS: UPLC-Q-TOF-MS was used to detect the main medicinal components and conduct quality control of YGY. A total of 60 rats were randomly divided into 2 groups: the NC group (10 rats) and the CKD model group (50 rats). The CKD model rats was established by administration of adenine 150 mg kg-1 orally for 14 days. After that, the CKD rats were randomly divided into 5 groups: the CKD group, YGY (10 g kg-1 group, 20 g kg-1 group, 40 g kg-1 group) and the GUI-LU-ER-XIAN-JIAO (GL) 10 g kg-1 group with 10 rats in each group. From the 15th day to the 45th day rats were given 150 mg kg-1 adenine orally every other day to maintain the model (except in the NC group). The YGY groups and the GL group were orally administered the relevant drug once per day for 30 days. The NC group and the CKD group were orally administered an equal volume of normal saline for 30 days. On the 45th day, the rats' sexual behavior index was tested. On the 46th day, the rats were sacrificed. Biochemical indexes, histopathological changes of the kidneys and testes, sperm morphology, sperm abnormality rate, and key proteins in the HIF1α-STAT5 pathway in the kidney and testis were detected. RESULTS: Thirteen components in the YGY extract were identified by UPLC-Q-TOF-MS for quality control of the YGY extract. The results of the biochemical and physiological tests validated the success of inducing CKD accompanied by reproductive dysfunction in rats. YGY significantly retarded the CKD progression and improved the hormone levels of male CKD rats. Sexual behavior tests showed YGY can significantly improve CKD rats' sexual function. In addition, the pathological changes of the kidney and testis, sperm abnormality rate and sperm morphological abnormalities of the CKD rats were reduced by YGY. Furthermore, decreased expression of HIF1α and EPO, and increased expression of p-EPOR (Tyr368), p-JAK2 (Tyr570) and p-STAT5 (Ser725) were observed in the kidney and the testis of the CKD rats. The YGY extract dramatically increased the expression of HIF1α and EPO, and decreased the expression of p-EPOR (Tyr368), p-JAK2 (Tyr570) and p-STAT5 (Ser725) to regulate key proteins in the HIF1α-STAT5 pathway of the kidney and testis. CONCLUSIONS: YGY has obvious reversal effects on the abnormal symptoms of adenine-induced CKD and the abnormal symptoms of rats with hypothyroidism and male reproductive hypotension. Its mechanism is related to its ability to regulate the HIF1α-STAT5 pathway.

2.
J Exp Clin Cancer Res ; 38(1): 438, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666108

RESUMO

BACKGROUND: Glutathione S-transferase zeta 1 (GSTZ1) is the penultimate enzyme in phenylalanine/tyrosine catabolism. GSTZ1 is dysregulated in cancers; however, its role in tumorigenesis and progression of hepatocellular carcinoma (HCC) is largely unknown. We aimed to assess the role of GSTZ1 in HCC and to reveal the underlying mechanisms, which may contribute to finding a potential therapeutic strategy against HCC. METHODS: We first analyzed GSTZ1 expression levels in paired human HCC and adjacent normal tissue specimens and the prognostic effect of GSTZ1 on HCC patients. Thereafter, we evaluated the role of GSTZ1 in aerobic glycolysis in HCC cells on the basis of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Furthermore, we assessed the effect of GSTZ1 on HCC proliferation, glutathione (GSH) concentration, levels of reactive oxygen species (ROS), and nuclear factor erythroid 2-related factor 2 (NRF2) signaling via gain- and loss- of GSTZ1 function in vitro. Moreover, we investigated the effect of GSTZ1 on diethylnitrosamine (DEN) and carbon tetrachloride (CCl4) induced hepatocarcinogenesis in a mouse model of HCC. RESULTS: GSTZ1 was downregulated in HCC, thus indicating a poor prognosis. GSTZ1 deficiency significantly promoted hepatoma cell proliferation and aerobic glycolysis in HCC cells. Moreover, loss of GSTZ1 function depleted GSH, increased ROS levels, and enhanced lipid peroxidation, thus activating the NRF2-mediated antioxidant pathway. Furthermore, Gstz1 knockout in mice promoted DEN/CCl4-induced hepatocarcinogenesis via activation of the NRF2 signaling pathway. Furthermore, the antioxidant agent N-acetylcysteine and NRF2 inhibitor brusatol effectively suppressed the growth of Gstz1-knockout HepG2 cells and HCC progression in Gstz1-/- mice. CONCLUSIONS: GSTZ1 serves as a tumor suppressor in HCC. GSH depletion caused by GSTZ1 deficiency elevates oxidative stress, thus constitutively activating the NRF2 antioxidant response pathway and accelerating HCC progression. Targeting the NRF2 signaling pathway may be a promising therapeutic approach for this subset of HCC.

3.
PLoS One ; 14(10): e0223169, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31609968

RESUMO

High levels of self-control are found to be associated with greater life satisfaction. To further understand this relationship, the current study examined two questions: (1) whether too much self-control reduces, rather than increases, life satisfaction, as argued by some scholars; and (2) whether engaging in prosocial behavior explains the "self-control-life satisfaction" link. To this end, we conducted survey research among adolescents (N = 1,009), university students (N = 2,620), and adult workers (N = 500). All participants answered the same self-control and life satisfaction measures, whereas prosocial behavior was assessed using different scales across samples. Results of two-line regressions failed to reveal significant inverted-U shaped association between self-control and life satisfaction across samples. Moreover, results of mediation analyses showed that across samples, high levels of self-control were related to greater life satisfaction and this association was partly mediated by prosocial behavior. In conclusion, there is no evidence showing that too much self-control impairs life satisfaction. Engaging in prosocial behavior partly explains how high self-control relates to greater well-being.

4.
J Sep Sci ; 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31610075

RESUMO

Fish oils are rich in eicosapentaenoic acid which has the wide-ranging biological activities. The rapid and efficient separation of eicosapentaenoic acid ethyl ester from fish oils ethyl ester is still regarded as a challenge. In this study, we described an effective and flexible chromatography for eicosapentaenoic acid ethyl ester preparation, named continuous batch chromatography, which combined the batch chromatography with the continuous chromatographic operation mode. After continuous batch chromatography experiment, the recovery of eicosapentaenoic acid ethyl ester was 82.01%, the average relative purity and the relative highest purity of eicosapentaenoic acid ethyl ester were 97.82% and 98.98%. The productivity of continuous batch chromatography was 5.48 times higher than that of batch chromatography, while the solvent consumption of eicosapentaenoic acid ethyl ester was 78% of the batch chromatography. This paper provided a reference for the separation of the targeted chemical component from multi-component mixtures. This article is protected by copyright. All rights reserved.

5.
Cell Signal ; 65: 109423, 2019 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-31639491

RESUMO

Osteoarthritis (OA) is the most common disease of joint tissues; unfortunately, there are currently no curative therapies available for OA. Chondrocytes, the only cell type residing in cartilage, secrete many types of collagen (the mainly one is type II collagen) and aggrecan, which are the main components of the cartilage matrix. Chondrocyte apoptosis can lead to OA degenerative progression. We previously indicated that recombinant human midkine (rhMK), as a chondrocyte growth factor has a significant reparative effect on cartilage injury animal models. However, the molecular mechanism of this restorative function remains under investigation. Herein, we focused on the molecular mechanism underlying the role of MK in promoting the proliferation of chondrocytes cultured in vitro. Chondrocytes from rats and OA patients were successfully isolated by the digestion of articular cartilage using type II collagenase, and their proliferation was evaluated by a CCK8 assay and flow cytometry. rhMK stimulated the proliferation of chondrocytes from both OA patients and rats. Furthermore, qRT-PCR, shRNA-mediated knockdown, Western blot and immunoprecipitation (IP) assays were performed to identify the receptor and key elements responsible for the role of MK in promoting chondrocyte proliferation. Low-density lipoprotein receptor-related protein 1 (LRP1) was identified as the dominant MK receptor in chondrocytes that, as a translocator, mediates the endocytosis of MK. After being transferred into chondrocytes, MK was shown to form a complex with nucleolin that interacts with the active form of K-Ras. Upon the activation of ERK1/2, cyclin D1 expression was upregulated, promoting the chondrocyte cell cycle. Our data reveal for the first time the role of the MK-LRP1-nucleolin signaling pathway in facilitating MK-induced chondrocyte proliferation, thus providing a strong theoretical foundation for the further use of MK in OA clinical therapy.

6.
Phys Chem Chem Phys ; 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31657411

RESUMO

A series of MIL-101-SO3H/Nafion composite membranes was synthesized. They show an improved proton conductivity, due to the abundance of SO3H groups, which fosters proton conduction by binding the water molecules and enabling a larger number of conducting sites. Gas (including water vapor, hydrogen, and oxygen) permeability, crystallinity, and free volumes of the MIL-101-SO3H/Nafion composite membranes were investigated, as well as their correlation. By increasing the MIL-101-SO3H content, the gas permeability of the membranes significantly decreases, since the crystalline region is larger and the water-bearing MIL-101-SO3H particles are efficient barriers for the gas molecules. The gas permeation in the composite membranes is a very complex process and the results indicate no simple linear relation between the gas permeability and the free volume size (VFV), or between the gas permeability and the crystallinity. Moreover, it is very interesting to observe that the influence of VFV on the gas permeability is closely related to the size of the particular gas molecules: the larger the size of the gas molecules, the larger the free volume needed to achieve their rapid diffusion in the membrane. The results suggest the presence of a threshold value for VFV, which depends on the size of the gas molecules: when VFV is lower than this value, the gas molecules cannot easily jump through neighboring free volumes to a neighboring site, and, as a result, the permeability drops quickly.

7.
Int J Biol Sci ; 15(11): 2350-2362, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31595153

RESUMO

The interaction between genomic DNA and protein fundamentally determines the activity and the function of DNA elements. Capturing the protein complex and identifying the proteins associated with a specific DNA locus is difficult. Herein, we employed CRISPR, the well-known gene-targeting tool in combination with the proximity-dependent labeling tool BioID to capture a specific genome locus associated proteins and to uncover the novel functions of these proteins. By applying this research tool on telomeres, we identified DSP, out of many others, as a convincing telomere binding protein validated by both biochemical and cell-biological approaches. We also provide evidence to demonstrate that the C-terminal domain of DSP is required for its binding to telomere after translocating to the nucleus mediated by NLS sequence of DSP. In addition, we found that the telomere binding of DSP is telomere length dependent as hTERT inhibition or knockdown caused a decrease of telomere length and diminished DSP binding to the telomere. Knockdown of TRF2 also negatively influenced DSP binding to the telomere. Functionally, loss of DSP resulted in the shortened telomere DNA and induced the DNA damage response and cell apoptosis. In conclusion, our studies identified DSP as a novel potential telomere binding protein and highlighted its role in protecting against telomere DNA damage and resultant cell apoptosis.

8.
Int J Biol Sci ; 15(11): 2381-2392, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31595156

RESUMO

Lung cancer is a malignancy with high morbidity and mortality worldwide. More evidences indicated that gut microbiome plays an important role in the carcinogenesis and progression of cancers by metabolism, inflammation and immune response. However, the study about the characterizations of gut microbiome in lung cancer is limited. In this study, the fecal samples were collected from 16 healthy individuals and 30 lung cancer patients who were divided into 3 groups based on different tumor biomarkers (cytokeratin 19 fragment, neuron specific enolase and carcinoembryonic antigen, respectively) and were analyzed using 16S rRNA gene amplicon sequencing. Each lung cancer group has characterized gut microbial community and presents an elimination, low-density, and loss of bacterial diversity microbial ecosystem compared to that of the healthy control. The microbiome structures in family and genera levels are more complex and significantly varied from each group presenting more different and special pathogen microbiome such as Enterobacteriaceae, Streptococcus, Prevotella, etc and fewer probiotic genera including Blautia, Coprococcus, Bifidobacterium and Lachnospiraceae. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and COG annotation demonstrated decreased abundance of some dominant metabolism-related pathways in the lung cancer. This study explores for the first time the features of gut microbiome in lung cancer patients and may provide new insight into the pathogenesis of lung cancer system, with the implication that gut microbiota may serve as a microbial marker and contribute to the derived metabolites, development and differentiation in lung cancer system.

9.
Nurs Ethics ; : 969733019876298, 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31631767

RESUMO

BACKGROUND: The three-dimensional model of nurses' moral sensitivity has typically been studied using a variable-centered rather than a person-centered approach, preventing a more complete understanding of how these forms of moral sensitivity are expressed as a whole. Latent profile analysis is a person-centered approach that classifies individuals from a heterogeneous population into homogeneous subgroups, helping identify how different subpopulations of nurses use distinct combinations of different moral sensitivities to affect their service behaviors. OBJECTIVE: Latent profile analysis was used to identify three distinct profiles of nurses' moral sensitivity. Associations of the profiles with service behaviors were then examined. METHODS: Five hundred twenty-five nurses from three tertiary hospitals in China were investigated with Moral Sensitivity Questionnaire and Nurses' Service Behavior Scale. Latent profile analysis was used to analyze the data. ETHICAL CONSIDERATIONS: Approval was obtained from the Ethics committee for biomedical research of Medical College, the Hebei University of Engineering. RESULTS: A three-profile moral sensitivity model provided the best fit to the data. The resulting profiles were low moral sensitivity, moderate moral sensitivity, and high moral sensitivity. There were significant differences in service behaviors among different profiles of moral sensitivity. CONCLUSION: The results provide a new and expanded view of nurses' moral sensitivity, which may be used to monitor nurses' service behaviors comprehensively and to evaluate nursing ethics management strategies.

10.
Chemistry ; 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31633249

RESUMO

The coordination number between copper and nitrogen in copper-nitrogen based electrocatalysts is quite important for boosting the oxygen reduction reaction (ORR). However, it is difficult to control the unsaturated copper-nitrogen constructions as well as compare their ORR performance in a similar carbon matrix by a simple but efficient way. Herein, we easily attained two types of Cu + -N 2 and Cu 2+ -N 4 constructions just by etching the pyrolyzed Cu-doped zeolitic imidazolate framework nanoleaf (Cu-ZIF-L) with sulfuric acid or nitric acid, respectively. X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) spectra were adopted to further confirm the different copper-nitrogen constructions after different acid treatment. By comparison, electrochemical studies demonstrate that Cu + -N 2 sites get higher activity for boosting the ORR performance than that of Cu 2+ -N 4 sites. Furthermore, Cu-N/C-H 2 SO 4 acted as air cathode in the Zinc-air battery exhibits an excellent performance and stability.

11.
Stem Cell Res Ther ; 10(1): 295, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547872

RESUMO

BACKGROUND: Interleukin 33 is known to have an important influence in the process of myocardial infarction, and the immunoregulatory function of MSCs could be influenced by cell factors. In this study, we evaluated the therapeutic efficacy of IL-33-overexpressing bone marrow mesenchymal stem cells (IL33-MSCs) on myocardial infarction (MI) and detected the inflammatory level and cardiac function in rats. METHODS AND RESULTS: First, we evaluated the proliferation of T cells and polarization of macrophages that had been co-cultured with Vector-MSCs or IL33-MSCs. Co-culture experiments indicated that IL33-MSCs reduced T cell proliferation and enhanced CD206+ macrophage polarization. Second, we determined the inflammation level and cardiac function of PBS-, Vector-MSC-, and IL33-MSC-injected rats. Echocardiography indicated that left ventricular ejection fraction (LVEF) was enhanced in IL33-MSC-injected rats compared with Vector-MSC-injected rats. Postmortem analysis of rat heart tissue showed reduced fibrosis and less inflammation in IL33-MSC-injected rats. CONCLUSION: These studies indicated that the IL33-MSC injection improved heart function and reduces inflammation in rats with MI compared with PBS or Vector-MSC injections. IL-33 overexpression enhances the immunomodulatory function and therapeutic effects of MSCs on acute MI via enhancing the polarization of macrophages toward M2, enhancing the differentiation of CD4+ T cells toward CD4+IL4+Th2 cells, and finally, reducing heart inflammation and enhancing heart function.

12.
Mater Sci Eng C Mater Biol Appl ; 105: 110086, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546413

RESUMO

Chitosan (CS) has been reported to have utility for various potential applications in biomedicine, tissue engineering, and cosmetics, as well as in the formulation of antibacterial agents because it exhibits a variety of desirable attributes, including low-toxicity, biodegradability, excellent biocompatibility, and broad-spectrum antibacterial activity. However, the full realization of CS's biomedical applications are practically constrained by its poor solubility. The goal of the present study is to prepare hydroxybutyl chitosan (HBCS) and investigate its impacts on immunocompetence, and its antibacterial activity. In the current study, HBCS was synthesized by modifying the hydroxybutyl group on the chitosan molecule using an etherification method. The physicochemical properties of the synthesized HBCS were characterized by various methods. Results showed that hydro-soluble HBCS exhibited excellent hygroscopicity and moisture retention. It was also found that HBCS exhibited notable cytocompatibility when cultured with mouse embryo fibroblasts. HBCS was able regulate immuno-functionality and promote immunocompetence by improving phagocytosis of macrophages and reinforcing lymphocyte activity in vivo and in vitro. Moreover, HBCS was also found to inhibit L-929 cell migration, indicating the impeded migration and metastasis behaviors of fibrosarcoma cells. Additionally, HBCS displayed favorable antimicrobial functionality against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. This study demonstrated that HBCS could in turn lock moisture, can promote immunocompetence activity, can inhibit fibrosarcoma cell migration, and exhibits anti-bacterial functionality. Taken together, these results indicate that HBCS shows substantial promise for applications in cosmetics, biomedicine, and antibacterial therapies.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31536001

RESUMO

Recent years have witnessed the wide application of hashing for large-scale image retrieval, because of its high computation efficiency and low storage cost. Particularly, benefiting from current advances in deep learning, supervised deep hashing methods have greatly boosted the retrieval performance, under the strong supervision of large amounts of manually annotated semantic labels. However, their performance is highly dependent upon the supervised labels, which significantly limits the scalability. In contrast, unsupervised deep hashing without label dependence enjoys the advantages of well scalability. Nevertheless, due to the relaxed hash optimization, and more importantly, the lack of semantic guidance, existing methods suffer from limited retrieval performance. In this paper, we propose a SCAlable Deep Hashing (SCADH) to learn enhanced hash codes for social image retrieval. We formulate a unified scalable deep hash learning framework which explores the weak but free supervision of discriminative user tags that are commonly accompanied with social images. It jointly learns image representations and hash functions with deep neural networks, and simultaneously enhances the discriminative capability of image hash codes with the refined semantics from the accompanied social tags. Further, instead of simple relaxed hash optimization, we propose a discrete hash optimization method based on Augmented Lagrangian Multiplier to directly solve the hash codes and avoid the binary quantization information loss. Experiments on two standard social image datasets demonstrate the superiority of the proposed approach compared with stateof- the-art shallow and deep hashing techniques.

14.
Biomed Pharmacother ; 120: 109476, 2019 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-31563816

RESUMO

Papillary thyroid carcinoma (PTC) is a common endocrine tumor. This study found that different iodine concentrations affected the proliferation, apoptosis, and migration of PTC. For this study, we collected clinical information from PTC patients and monitored the levels of urinary iodine, LC3-II, and caspase-3 in cancer tissue, and BRAF kinase in peripheral blood from PTC patients. We also monitored the proliferation, apoptosis and migration ability of human papillary-thyroid carcinoma (BCPAP) cells at different iodine concentrations and their association with changes in autophagy and BRAF kinase activity of BCPAP cells at high iodine levels (10-3 mol/l). We found that the proportion of tumor diameters ≥ 1 cm in the iodine excess group were lower than that in the iodine non-excess group. The proportion of PTC patients with infiltration in the iodine excess group was higher than that in the iodine non-excess group. Levels of the autophagy-related protein LC3-II and the apoptosis-related protein caspase-3 in cancer tissues, and activity of BRAF kinase in peripheral blood, were positively correlated with urinary iodine concentrations from PTC patients. At high iodine levels, the proliferation rate decreased, and apoptosis percentage and migration rates increased compared with the no-iodine group. At high iodine levels, the frequencies of autophagosomes (Aph) and autophagosome-lysosomes (Apl) in BCPAP cells increased significantly, and activities of LC3-II and BRAF kinase increased, respectively. The activity of LC3-II decreased when BRAF kinase was inhibited. The activity of LC3-II and the proliferation and migration rates of BCPAP cells decreased, and the apoptosis percentage increased when autophagy was inhibited at high iodine concentrations. Our results demonstrated that, in the presence of excessive iodine, the mean tumor size of PTC patients was smaller and easier to invade than tumors in patients not supplied with excessive iodine. The levels of autophagy and apoptosis in PTC cancer tissues, and activities of BRAF kinase in peripheral blood increased with increasing urinary iodine concentrations. High iodine levels inhibited cell proliferation and promoted apoptosis and migration of PTC cells. Autophagy induced by BRAF kinase in PTC cells was involved in anti-apoptosis, and promoted proliferation and migration at high iodine concentrations. This study provides a rationale for iodine supplementation in PTC patients.

15.
Med Sci Monit ; 25: 7042-7051, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31536481

RESUMO

BACKGROUND Late diagnosis and metastasis are leading causes of the high mortality of colorectal cancer (CRC). Long noncoding RNAs (lncRNAs) have been reported to play a critical role in the development and progression of CRC. This study aimed to explore the clinical significance of 2 novel lncRNAs - RP11-296E3.2 and LEF1-AS1 - including their expression pattern, as well as diagnostic and prognostic values, for metastatic CRC patients. MATERIAL AND METHODS lncRNAs expression was examined in tissues (91 cases) and plasma (60 cases) from CRC patients by real-time quantitative PCR (qRT-PCR), and the correlations between its expression and clinicopathological features and diagnosis values in metastasis were analyzed. TCGA datasets were further used to analyze their utility in prediction of overall survival (OS) and disease-free survival (DFS). ATP-based tumor chemosensitivity assay (ATP-TCA) was used to evaluated tumor chemoresistance. RESULTS Compared with adjacent normal tissues, RP11-296E3.2 was significantly downregulated while LEF1-AS1 was significantly upregulated in cancer tissues (p=0.0143, p=0.0322, respectively). High levels of RP11-296E3.2 and LEF1-AS1 in tissues and plasma were correlated with tumor metastasis (p=0.0488, p=0.0252 in tissues, p=0.0331, p=0.1862 in plasma, respectively). Further analysis showed that RP11-296E3.2 sensitivity and specificity in diagnosis of CRC metastasis is better than CEA in plasma (0.690 and 0.621, and 0.621 and 0.500, respectively), and the OS of metastatic CRC patients with higher LEF1-AS1 expression levels in tissues was short (log-rank p<0.05). CONCLUSIONS Our findings suggest that RP11-296E3.2 and LEF1-AS1 could separately serve as potential novel diagnosis and prognostic markers for CRC metastasis.

16.
Fish Shellfish Immunol ; 94: 580-591, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31539571

RESUMO

A STAT gene from Scylla paramamosain, named SpSTAT, was cloned and characterized. The full length of SpSTAT mRNA contains a 5'untranslated region (UTR) of 238 bp, an open reading frame (ORF) of 2388 bp and a 3' UTR of 326 bp. The SpSTAT protein contains four characteristic STAT domains and showed 84% identity (90% similarity) and 44% identity (64% similarity) to Litopenaeus vannamei STAT protein and Human STAT5a/b protein, respectively. The mRNA of SpSTAT was high expressed in the intestine and eyestalk and low expressed in the heart and muscle. Moreover, expression of SpSTAT was significantly responsive to challenge of mud crab reovirus (MCRV), Poly(I:C), LPS and Staphylococcus aureus. SpSTAT could be activated by Poly(I:C) and LPS to translocate to the nucleus of Drosophila Schneider 2 (S2) cells. SpSTAT could be phosphorylated by interaction with JAK of S. paramamosain (SpJAK) and activated to translocate to the nucleus of S2 cells. Furthermore, Silencing of SpSTAT in vivo resulted in higher mortality rate of MCRV infected mud crab and increased the viral load in tissues, suggesting that SpSTAT could play an important role in defense against MCRV in mud crab.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31518715

RESUMO

BACKGROUND & AIMS: Chromoendoscopy with iodine staining is used to identify esophageal squamous dysplasia and esophageal squamous cell carcinomas (ESCCs)-absence of staining indicates suspicious regions of dysplasia. However, screening detects precancerous lesions (mild and moderate dysplasia) that do not require immediate treatment; it is a challenge to which lesions are at risk for progression. We investigated the association between absence of iodine staining at chromoendoscopy screening and lesion progression using 6 years of follow-up data from a population-based randomized controlled trial in China. We then constructed and validated a model to calculate risk of progression to severe dysplasia, carcinoma in situ, or ESCC. METHODS: We collected data from 1468 participants (45-69 years old) who were either negative for iodine staining at a baseline chromoendoscopy or found to have mild or moderate dysplasia in histologic analysis of biopsies in the Endoscopic Screening for Esophageal Cancer study in China, from January 2012 through September 2016; 788 of these participants were re-examined by endoscopy after a median interval of 4.2 years (development cohort). We investigated the association between absence of iodine staining and progression of esophageal lesions using Cox prediction models, considering corresponding baseline pathology findings and patient answers to a comprehensive questionnaire. Patients who did not receive a follow-up examination (n = 680) was used as the validation cohort; outcome events in these patients were identified by annual door to door active interviews or linkage with local electronic registry data. The primary outcome was incident esophageal severe dysplasia, carcinoma in situ, or ESCC. RESULTS: In the development cohort, 11 lesions that did not stain with iodine but were classified as not dysplastic in the histology analysis were found to be severe dysplasia, carcinoma in situ, or ESCC at the follow-up evaluation. These lesions accounted for 39.3% of all progressed lesions (n = 28). In the validation cohort, 6 patients with lesions did not stain with iodine but were classified as not dysplastic by histology had a later diagnosis of ESCC, determined from medical records; these patients accounted for 50.0% of all patients with lesion progression (n = 12) until the closing date of this study. We developed a model based on patient age, body mass index, pathology findings, and baseline iodine staining to calculate risk for severe dysplasia, carcinoma in situ, or ESCC. It identified patients for severe dysplasia, carcinoma in situ, or ESCC in the development set with an area under the curve of 0.868 (95% CI, 0.817-0.920) and in the validation set with an area under the curve of 0.850 (95% CI, 0.748-0.952). Almost no cases would be missed if subjects determined to be high or intermediate-high risk subjects by the model were included in surveillance. CONCLUSIONS: Absence of iodine staining at baseline chromoendoscopy identifies esophageal lesions at risk of progression with a high level of sensitivity. A model that combines results of iodine chromoendoscopy with other patient features identifies patients at risk of lesion progression with greater accuracy than histologic analysis of baseline biopsies.

18.
Virol J ; 16(1): 106, 2019 08 22.
Artigo em Inglês | MEDLINE | ID: mdl-31438971

RESUMO

BACKGROUND: Plant viruses can affect vector's behaviors in order to enhance viral transmission. Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus) is an emergent RNA plant virus and is transmitted specifically by biotypes B and Q of tobacco whitefly, Bemisia tabaci (Gennadius), in a semipersistent manner. METHODS: We used the electrical penetration graph (EPG) to investigate the effect of CCYV on the feeding behaviors of B. tabaci biotypes B and Q. RESULTS: CCYV could affect, both directly and indirectly, the feeding behaviors of B. tabaci to various degrees, depending on biotypes and sexes of the insect. CCYV showed stronger direct effects on biotype Q than on biotype B in terms of increased non-phloem probing and phloem salivation. CCYV increased non-phloem probing and phloem salivation more on females than on males of biotype Q, and increased phloem salivation more on females than on males of biotype B. CCYV had stronger indirect effects, via virus-infested plants, on biotype B than on biotype Q by enhancing phloem sap ingestion and feeding bouts. CCYV increased non-phloem probing and feeding bouts more on males than on females of biotype B, and decreased phloem sap ingestion more on males than on females on biotype Q indirectly. CONCLUSIONS: The results clearly indicated that CCYV affects the feeding behaviors of B. tabaci, which may lead to increased ability of the B. tabaci for CCYV transmission.

19.
J Microbiol Methods ; 164: 105684, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31394120

RESUMO

RNA-based molecular technique (RT-qPCR) is a promising method for microcystin monitoring in lakes and reservoirs, but great lability of RNA in cyanobacterial samples limits its application. To date, no studies have investigated how to effectively preserve RNA in cyanobacterial samples. In this study, four different treatments (-80 °C freezer, -196 °C liquid nitrogen, 4 °C or 25 °C preservation after adding RNA protective fluid) were employed to preserve RNA in pure culture and field Microcystis samples, and RNA degradation in these treatments were systematically evaluated. Results showed liquid nitrogen was the most effective treatment to preserve RNA in pure culture and field Microcystis samples. RNA preservation using RNA protective fluid was temperature dependent. Low temperature (4 °C) could effectively slow down RNA degradation within a short time (1-7 d), since decay rate of mcyH mRNA (k = 0.00094 d-1) was much lower at 4 °C than that at 25 °C (0.0549 d-1) (P < 0.05). However, for field samples, RNA degradation was much faster than pure culture samples with the same treatment. Therefore, to better preserve RNA in field samples, a practical strategy for RNA preservation combining RNA protective fluid and liquid nitrogen, was proposed. Tests of field experiments showed it was more effective than individual treatment for RNA preservation in Microcystis samples during field sampling. Thus, this strategy could be employed to preserve RNA in cyanobacterial samples during field sampling, which will contribute to the application of RT-qPCR technique for microcystin monitoring in lakes and reservoirs.

20.
Chemosphere ; 238: 124432, 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31421464

RESUMO

This study investigated the adsorption of tetracycline (TC) on biochar (BC) derived from waste Auricularia auricula dregs obtained at different pyrolysis temperatures. The characterization of BC and batch experiment results showed that BC prepared at a higher temperature was more suitable for removing TC, where the maximum adsorption capacities of BC samples prepared at 300 °C, 500 °C, and 700 °C were 7.22 mg/g, 9.90 mg/g, and 11.90 mg/g, respectively. A pseudo-first order kinetics model and Freundlich, Temkin, and Dubinin-Radushkevich isotherm models fitted well to the adsorption data. Liquid film diffusion was the rate-controlling step. In addition, π-π electron donor-acceptor interactions may have played a dominant role in the adsorption mechanism between the enone structure of TC and aromatic C of BC. These results may facilitate further investigations of the adsorption mechanism and optimization of the process.

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