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1.
Biosens Bioelectron ; 220: 114893, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36423391

RESUMO

Despite the extensive investigation of the nanozymes exhibit their favorable performance compared to natural enzymes, nevertheless, the highly specific nanozyme still needs to be developed so that it can meet the requirements of exploring the mechanism as well as administration of related diseases and selective monitoring in biological system. In this study, self-assembled glutathione-Cu/Cu2O nanoparticles (GSH-Cu/Cu2O NPs) that exhibits specific ascorbic acid (AA) oxidase-like catalytic activity were constructed for AA-activated and H2O2-reinforced cancer cell proliferation inhibition and selective neurochemical monitoring. Cu/Cu2O NPs demonstrates effective AA oxidase-like activity and no common characteristics of other redox mimic enzymes often present in nanozyme. In particular, we found that the AA oxidase-like activity of GSH-Cu/Cu2O nanozyme was significantly improved by about 40% by improving the activation ability toward oxygen. The synthesized nanozyme can induce the generation of active oxygen by accelerating the oxidation of AA, which effectively suppresses the proliferation of cancer cells. We constructed an online electrochemical system (OECS) though loading nanozyme with enhanced ascorbate oxidase activity into a microreactor and setting it in the upstream of the detector. This GSH-Cu/Cu2O NPs-integrated microreactor can completely eliminate AA interference of the physical level toward 3,4-dihydroxy phenylacetic acid (DOPAC) electrochemical measurement, and the nanozyme-based OECS is able to continuously capture DOPAC alteration in rat brain acidosis model. Our findings may inspire rational design of nanozymes with high specificity as well as nanozyme-based selectivity solution for in vivo detection and show promising opportunities for their involvement in neurochemistry investigation.


Assuntos
Técnicas Biossensoriais , Neoplasias , Animais , Ratos , Ascorbato Oxidase , Ácido 3,4-Di-Hidroxifenilacético , Peróxido de Hidrogênio , Proliferação de Células , Ácido Ascórbico , Glutationa
2.
J Hazard Mater ; 443(Pt B): 130318, 2023 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-36444052

RESUMO

In this study, CuO@SiO2 core-shell catalysts were successfully synthesized and applied to efficiently remove hazardous gaseous pollutant arsine (AsH3) by catalytic oxidation under low-temperature and low-oxygen conditions for the first time. In typical experiments, the CuO@SiO2 catalysts showed excellent AsH3 removal activity and stability under low-temperature and low-oxygen conditions. The duration of the AsH3 conversion rate above 90 % for the CuO@SiO2 catalysts was 39 h, which was markedly higher than that of other catalysts previously reported in the literature. The considerable catalytic activity and stability were attributed to the protection and confinement effects of the SiO2 shell, which resulted in highly dispersed CuO nanoparticles. Meanwhile, the strong interaction between the CuO core and SiO2 shell further facilitated the formation of active species such as coordinatively unsaturated Cu2+ and chemisorbed oxygen. The accumulation of oxidation products (As2O3 and As2O5) on the interface between the CuO core and SiO2 shell and the pore channels of the SiO2 shell is the main cause of catalysts deactivation. Furthermore, through combined density functional theory (DFT) calculations and characterization methods, a reaction pathway including gradual dehydrogenation (AsH3*→AsH2*→AsH*→As*) and gradual oxidation (2As*→As*+AsO*→2AsO*→As2O3) for the catalytic oxidation of AsH3 on CuO (111) surface was constructed to clarify the detailed reaction mechanism. The CuO@SiO2 core-shell catalysts applied in this study could provide a powerful method for developing AsH3 catalysts from multiple know AsH3 removal systems.

3.
J Hazard Mater ; 442: 130029, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36166909

RESUMO

Organic sulfur gases (COS, CS2 and CH3SH) are widely present in reducing industrial off-gases, and these substances pose difficulties for the recovery of carbon monoxide and other gases. The reaction pathways and reaction mechanisms of organic sulfur on different catalyst surfaces have yet to be fully summarized. The literature shows that many factors, such as catalyst synthesis method, loaded metal composition, number of surface hydroxyl groups, number of acid-base sites and methods of surface modification, have important effects on the catalytic performance of metal catalysts. Therefore, this paper presents a comprehensive review of the research on the application of catalysts such as zeolites, metal oxides, carbon-based materials, and hydrotalcite-like derivatives in the field of organic sulfur removal. Future research prospects are summarized, more in situ characterization experiments and theoretical calculations are needed for the catalytic decomposition of methanethiol to analyze the coke generation pathways at the microscopic level, while the simultaneous removal of multiple organic sulfur gases needs to be focused on. Based on previous catalyst research, we propose possible innovations in catalyst design, desulfurization technology and organic sulfur resource utilization technology.

4.
Sci Total Environ ; 855: 158981, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36155044

RESUMO

Some personal care products (PCPs) and their chemical components showed a hormetic effect in the freshwater photobacterium Vibrio qinghaiensis sp. -Q67 (Q67) after long-term exposure. However, how hormesis transfers between chemical components and PCP mixture, and which chemical component plays a major role remain unknown. To this end, according to the seven compounds detected in one skin lotion (SK5) and their concentration ratios, many mixture rays were constructed to simulate the SK5. Of these seven compounds, three presented monotonic concentration-response curves (CRC) to Q67 at 0.25 and 12 h (called a S-shaped compound). The other four compounds showed hormetic CRCs after 12 h and monotonic CRCs at 0.25 h (called a J-shaped compound). Based on their mixture ratios, we designed one ternary mixture ray of all S-shaped compounds, one quaternary mixture ray of all J-shaped compounds, and four quaternary mixture rays of one J-shaped and three S-shaped compounds. It was shown that SK5 could be approximately simulated by the mixture ray of the seven compounds detected in SK5 and only the mixture rays containing at least one hormesis-inducing compound produced hormesis to Q67 at 12 h. Based on the concentration ratios of various compounds and comparison of four hormetic characteristic parameters to those of various mixture rays, it was found that the compound betaine (BET) is a key compound affecting the hormesis of mixtures. Additionally, we studied the hormesis mechanism of BET on Q67 via quorum sensing (QS). This preliminarily indicated that the autoinducer-2 triggered the QS pathway. This study elucidated the transfer pattern of hormesis into mixtures, which would be an efficient method to identifying the potential components that affect hormesis transfer in mixtures. We expect that this study will provide new insights into hormesis and its mixtures.


Assuntos
Cosméticos , Hormese
5.
Waste Manag ; 155: 311-319, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36410148

RESUMO

Meeting the current demand for concrete requires not only mining tons of gravel and sand, but also burning large amounts of fossil fuel resources in cement kilning. Consequently, concrete recycling is crucial to achieving a material-efficient society, especially with the application of various categories of concrete and the goal of phasing out fossil fuels. A comparative life cycle assessment (LCA) is used to assess the engineering material footprint (EMF) and the fossil fuel material footprint (FMF) in closed-loop recycling of three types of concrete: siliceous concrete, limestone concrete, and lightweight aggregate concrete. This study aims to investigate the impact of (i) concrete categories, (ii) methods to model recycling, and (iii) using renewable energy sources on the material footprint in concrete recycling. The results highlight that the concrete recycling system can reduce 99% of the EMF and 66-93% of the FMF compared with the baseline system, in which concrete waste is landfilled. All three recycling modeling approaches indicate that concrete recycling can considerably reduce EMF and FMF compared with the baseline system, primarily resulting from the displacement of virgin raw materials. Using alternative diesels is more sensitive than adopting renewable electricity in reduction of the FMF in concrete recycling. Replacing diesel with electrolysis- and coal-based synthetic diesel for concrete recycling could even increase the FMF, while using biodiesel made from rapeseed and wood-based synthetic diesel can reduce 47-51% and 84-89% of the FMF, respectively, compared to the virgin diesel-based recycling system. Finally, we discussed the multifunctionality and rebound effects of recycling, and double-counting risk in material and energy accounting.


Assuntos
Combustíveis Fósseis , Reciclagem , Animais , Biocombustíveis , Carbonato de Cálcio , Estágios do Ciclo de Vida
6.
Environ Res ; 216(Pt 1): 114472, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36209785

RESUMO

BACKGROUND: Limited evidence suggests the association of air pollutants with a series of diabetic cascades including inflammatory pathways, glucose homeostasis disorder, and prediabetes and diabetes. Subclinical strategies for preventing such pollutants-induced effects remain unknown. METHODS: We conducted a cross-sectional study in two typically air-polluted Chinese cities in 2018-2020. One-year average PM1, PM2.5, PM10, SO2, NO2, and O3 were calculated according to participants' residence. GAM multinomial logistic regression was performed to investigate the association of air pollutants with diabetes status. GAM and quantile g-computation were respectively performed to investigate individual and joint effects of air pollutants on glucose homeostasis markers (glucose, insulin, HbA1c, HOMA-IR, HOMA-B and HOMA-S). Complement C3 and hsCRP were analyzed as potential mediators. The ABCS criteria and hemoglobin glycation index (HGI) were examined for their potential in preventive strategy. RESULTS: Long-term air pollutants exposure was associated with the risk of prediabetes [Prevalence ratio for O3 (PR_O3) = 1.96 (95% CI: 1.24, 3.03)] and diabetes [PR_PM1 = 1.18 (95% CI: 1.05, 1.32); PR_PM2.5 = 1.08 (95% CI: 1.00, 1.16); PR_O3 = 1.35 (95% CI: 1.03, 1.74)]. PM1, PM10, SO2 or O3 exposure was associated with glucose-homeostasis disorder. For example, O3 exposure was associated with increased levels of glucose [7.67% (95% CI: 1.75, 13.92)], insulin [19.98% (95% CI: 4.53, 37.72)], HOMA-IR [34.88% (95% CI: 13.81, 59.84)], and decreased levels of HOMA-S [-25.88% (95% CI: -37.46, -12.16)]. Complement C3 and hsCRP played mediating roles in these relationships with proportion mediated ranging from 6.95% to 60.64%. Participants with HGI ≤ -0.53 were protected from the adverse effects of air pollutants. CONCLUSION: Our study provides comprehensive insights into air pollutant-associated diabetic cascade and suggests subclinical preventive strategies.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Diabetes Mellitus , Insulinas , Estado Pré-Diabético , Humanos , Complemento C3 , Estado Pré-Diabético/etiologia , Estado Pré-Diabético/induzido quimicamente , Estudos Transversais , Proteína C-Reativa , Poluição do Ar/efeitos adversos , Poluição do Ar/análise , Poluentes Atmosféricos/toxicidade , Poluentes Atmosféricos/análise , Diabetes Mellitus/epidemiologia , Diabetes Mellitus/induzido quimicamente , Homeostase , Glucose , Material Particulado/toxicidade , Material Particulado/análise , Exposição Ambiental/efeitos adversos , Exposição Ambiental/análise , Dióxido de Nitrogênio/toxicidade , China/epidemiologia
7.
Bioresour Technol ; 367: 128282, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36368483

RESUMO

The application of bio-manganese (Mn) redox cycling for continuous removal of contaminants provides promise for addressing coexisting contaminants in groundwater, however, the feasibility of constructing Mn redox cycling system (MCS) through community assembly remains to be elucidated. In this study, Mn-reducing strain MFG10 and Mn-oxidizing strain MFQ7 synergistically removed 94.67 % of 17ß-estradiol (E2) within 12 h. Analysis of potential variations in Mn oxides suggested that MCS accelerated the production of reactive oxygen species (ROS) and Mn(III), which interacted to promote E2 removal. After continuous operation of the Mn ore-based immobilized bioreactor for 270 days, the experimental group (EG) achieved average removal efficiencies of 89.63 % and 97.57 % for NO3--N and E2, respectively. High-throughput sequencing results revealed complex symbiotic relationships in EG. Community assembly significantly enhanced the metabolic and physiological activity of the bioreactor, which promoting the expression of core functions including nitrogen metabolism, Mn cycling and organic matter resistance.


Assuntos
Manganês , Nitratos , Reatores Biológicos , Oxirredução , Estradiol , Óxidos de Nitrogênio
8.
Adv Sci (Weinh) ; : e2204843, 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36461760

RESUMO

The toxicity of heavy-metal Pb and instability of lead-based halide perovskite nanomaterials are main factors to impede their practical applications in the fields of solar cells, LEDs and scintillators. In this paper, all inorganic lead-free cesium manganese halide nanocrystals are synthesized in glass for the first time. Red photoluminescence with broad PL band, negligible self-absorption and a high photoluminescence quantum yield of 41.8% is obtained. In addition, modulating halide component can change the Mn2+ ions coordination environment to obtain tunable photoluminescence from red to green. More importantly, cesium manganese halide nanocrystals embedded glasses exhibit outstanding long-term stabilities. Theses cesium manganese halide nanocrystals embedded glasses are also highly stable against high energy irradiation and exhibit highly efficient radioluminescence, making them promising for high-resolution X-ray imaging. These results demonstrate that cesium manganese halide nanocrystals embedded glasses are promising eco-friendly candidates for applications in light-emitting diodes and scintillators.

9.
Environ Pollut ; : 120782, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36464120

RESUMO

Alterations in the concentrations of trace elements may play a vital role in Alzheimer dementia progression. However, previous research results are inconsistent, and there is still a lack of review on the relationship between all the studied-trace elements and AD from various perspectives of population-based studies. In this study, we systematically reviewed previous population-based studies and identified the altered trace elements in AD patients. We searched the Web of Science Core Collection, PubMed, and Scopus database, and ultimately included 73 articles. A bibliometric analysis was conducted to explore the evolution of the field from an epidemiological perspective. Bibliometric data such as trace elements, biological materials, detection methods, cognitive tests, co-occurrence and co-citation statistics are all analyzed and presented in a quantitative manner. The 73 included studies analyzed 39 trace elements in total. In a further meta-analysis, standardized mean differences (SMDs) of 13 elements were calculated to evaluate their altered in AD patients, including copper, iron, zinc, selenium, manganese, lead, aluminum, cadmium, chromium, arsenic, mercury, cobalt, and manganese. We identified four trace elements-copper (serum), iron (plasma), zinc (hair), and selenium (plasma)-altered in AD patients, with SMDs of 0.37 (95% confidence interval [CI]: 0.10, 0.65), -0.68 (95% CI: -1.34, -0.02), -0.35 (95% CI: -0.62, -0.08), and -0.61 (95% CI: -0.97, -0.25), respectively. Finally, we formed a database of various trace element levels in AD patients and healthy controls. Our study can help future researchers gain a comprehensive understanding of the advancements in the field, and our results provide comprehensive population-based data for future research.

11.
Front Nutr ; 9: 1058025, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466391

RESUMO

The vegetable oil industry is limited by the high cost of the refining process, and the camellia shells (CS) are beneficial to the development of the industry as a biomass raw material for camellia oil decolorization. In this study, CS-based p-doped porous activated carbon (CSHAC) obtained after the pyrolysis of H3PO4-laden CS-hydrochar (CSH) was used for the adsorption of carotenoids in camellia oil. The results showed that the adsorption efficiency of CSHAC for carotenoids was 96.5% compared to 67-87% for commercial decolorizers, and exhibited a fast adsorption rate (20 min). The results of adsorption isotherms indicated that the adsorption of carotenoids on CSHAC occurred through a multi-layer process. Furthermore, the analysis of adsorption kinetics showed that the adsorption of carotenoids by CSHAC was a complex process involving physical and chemical reactions, and chemisorption was the dominant kinetic mechanism. This superior performance of CSHAC in adsorbing carotenoids was attributed to its micro-mesoporous structure, hydrophobicity, and numerous active sites.

12.
Front Public Health ; 10: 1015166, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36466464

RESUMO

Background: Due to tremendous academic pressure, Chinese high school students suffer from severe depression, anxiety, and sleep disturbances. Moreover, senior high school students commonly face more serious mental health problems than junior high school students. However, the co-occurrence and internal relationships of depression, anxiety, and sleep disturbances clusters are scarcely examined among high students. Therefore, the current study inspected relationships between depression, anxiety, and sleep disturbance symptoms through network analysis and identified key symptoms bolstering the correlation and intensifying the syndromes. Methods: A total of 13,999 junior high school students (M age = 13.42 years, SD age = 1.35, 50% females) and 12,550 senior high school students (M age = 16.93 years, SD age = 1.67, 47% females) were recruited in Harbin. We constructed networks for all students, junior high group, and senior high group, including data from the Youth Self-rating Insomnia Scale-3 (YSIS-3), the Generalized Anxiety Disorder-2 (GAD-2), and the Patient Health Questionnaire-2 (PHQ-2). The indices of "strength" was used to identify symptoms' centrality, and "bridge strength" was used to find specific nodes that could bridge anxiety, depression, and sleep disturbance. Results: The networks of all students, junior high and senior high students, were stable and accurate. Among all networks, "Nervousness" (GAD1) had the highest strength, and "Nervousness"-"Excessive worry" (GAD1-GAD2) had the strongest correlation. "Nervousness" (GAD1) also functioned as the bridge symptom among junior high students, while "Sad mood" (PHQ2) among senior high students. Senior high students scored higher than junior high students on all items and had a tighter network structure. Conclusions: In networks consisting of anxiety, depression, and sleep disturbance, anxiety plays a conspicuous role in comorbidity among junior high school students, which transforms into depression among senior high school students. Treatments or interventions should be focused on these critical symptoms.


Assuntos
Depressão , Transtornos do Sono-Vigília , Feminino , Adolescente , Humanos , Lactente , Masculino , Depressão/epidemiologia , Transtornos de Ansiedade/epidemiologia , Ansiedade/epidemiologia , Transtornos do Sono-Vigília/epidemiologia , Estudantes , China/epidemiologia , Sono
13.
Front Physiol ; 13: 1008869, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467680

RESUMO

Background: Serum lactate is commonly measured in the perioperative period in patients who have undergone surgery for an acute type A aortic dissection (ATAAD). However, conflicting data has been reported as to whether lactate elevation is associated with short-term prognosis. The aim of the current study was to determine the association between perioperative arterial lactate levels and postoperative 30-day mortality. Methods: Patients who underwent repair of a ATAAD at our institution were retrospectively screened and those with comprehensive measurements of serum lactate before surgery and at 0, 1, 3, 6, 12, and 24 h after surgery in the intensive care unit (ICU) were selected for the analysis. Patients' demographic features and outcomes were reviewed to determine risk factors associated with 30-day mortality using logistic regression modeling. The association between serum lactate levels at different time points and 30-day mortality were analyzed by receiver-operating characteristic curves. Results: 513 patients were identified and retrospectively analyzed for this study including 66 patients (12.9%) who died within 30 days after surgery. Patients who died within 30 days after surgery had elevated lactate levels measured before surgery and at 0, 1, 3, 6, 12, and 24 h after their ICU stay. Lactate measured at 24 h post ICU admission (odds ratio, 2.131; 95% confidence interval, 1.346-3.374; p = 0.001) was a predictor of 30-day mortality. The area under the curve (AUC) for 30-day mortality with lactate levels at 12 h and 24 h post ICU stay were 0.820 and 0.805, respectively. Conclusion: Early elevation of lactate level is correlated with increased 30-day mortality in patients who received ATAAD surgical repair.

14.
Front Neurol ; 13: 959917, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36468047

RESUMO

Background: Stroke is among the leading causes of disability of worldwide. Gait dysfunction is common in stroke survivors, and substantial advance is yet to be made in stroke rehabilitation practice to improve the clinical outcome of gait recovery. The role of the upper limb in gait recovery has been emphasized in the literature. Recent studies proposed that four limbs coordinated interventions, coined the term "interlimb-coordinated interventions," could promote gait function by increasing the neural coupling between the arms and legs. A high-quality review is essential to examine the clinical improvement and neurophysiological changes following interlimb-coordinated interventions in patients with stroke. Methods: Systematic review and meta-analysis will be conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The literature will be retrieved from the databases of OVID, MEDLINE, PubMed, Web of Science, EMBASE, and PsycINFO. Studies published in English over the past 15 years will be included. All of the clinical studies (e.g., randomized, pseudorandomized and non-randomized controlled trials, uncontrolled trials, and case series) that employed interlimb intervention and assessed gait function of patients with stroke will be included. Clinical functions of gait, balance, lower limb functions, and neurophysiologic changes are the outcome measures of interest. Statistical analyses will be performed using the Comprehensive Meta-Analysis version 3. Discussion: The findings of this study will provide insight into the clinical benefits and the neurophysiological adaptations of the nervous system induced by interlimb-coordinated intervention in patients with stroke. This would guide clinical decision-making and the future development of targeted neurorehabilitation protocol in stroke rehabilitation to improve gait and motor function in patients with stroke. Increasing neuroplasticity through four-limb intervention might complement therapeutic rehabilitation strategies in this patient group. The findings could also be insightful for other cerebral diseases.

15.
Zhongguo Zhong Yao Za Zhi ; 47(22): 6199-6206, 2022 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-36471945

RESUMO

Metabolomics based on ultra-high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS) was employed to analyze the effect of Cyathulae Radix on serum and urine in rats with knee osteoarthritis, and to decipher the mechanism of Cyathulae Radix in the treatment of knee osteoarthritis. SD rats were randomized into a normal group, a model group, a positive drug group, and a Cyathulae Radix group. The knee osteoarthritis model was established by injecting 2% pa-pain and 0.03 mol·L~(-1) cysteine, and the serum levels of tumor necrosis factor-α(TNF-α) and matrix metalloproteinase-3(MMP-3) in the model group was measured to preliminarily evaluate the therapeutic effect of Cyathulae Radix on knee osteoarthritis. UHPLC-Q-TOF-MS was employed to establish the metabolic profile of endogenous small molecule metabolites in the four groups. Potential biomarkers were screened out by multivariate analysis methods such as partial least squares discriminant analysis(PLS-DA) and orthogonal partial least squares discriminant analysis(OPLS-DA) in combination with t-test, variable importance for projection(VIP), and fold-change. The related metabolic pathways were enriched with the help of MetaboAnalyst 5.0. The results showed that Cyathulae Radix alleviated the general signs of rats with knee osteoarthritis, and reduced the levels of TNF-α and MMP-3 in the rat serum. Twenty-eight differential metabolites that might be associated with the therapeutic effect of Cyathulae Radix were screened out from serum and urine. They were mainly involved in arginine biosynthesis, nicotinate and nicotinamide metabolism, tricarboxylic acid cycle, alanine, aspartate and glutamate metabolism, riboflavin metabolism, glyoxylate and dicarboxylate metabolism, and pyrimidine metabolism. Through metabonomics analysis, this study predicted the possible mechanism of Cyathulae Radix in the treatment of knee osteoarthritis, which laid a foundation for further research.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36472467

RESUMO

Online shopping addiction has surged among today's university students. Previous studies have focused on individual and network factors, whereas neglecting family-related roles. This study examined the relationship between subjective socioeconomic status (SSES) and university students' online shopping addiction (OSA) using the life course model of consumer behavior, and explored the chain-mediating role of vanity and materialism, and the moderating role of gender. We surveyed 635 students from two universities in Henan Province, China, using self-administered scales and questionnaires assessing subjective SES (SSES), OSA, materialism, and vanity. The results showed that SSES is negatively associated with OSA. Materialism played a mediating role, whereas vanity and vanity-materialism played a suppressing role between SSES and OSA. A direct relationship between SSES and OSA was found only in men, whereas the indirect path of SSES-vanity-materialism-OSA was found only in women. These results enable better recognition and understanding of the family's role, including family economic status, in university students' gender-specific OSA. They also advance the understanding of the transmission mechanism between family economic status and university students' OSA and promote better recognition and awareness of the family's role in university students' gender-specific OSA.

17.
Front Bioeng Biotechnol ; 10: 1072393, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452209

RESUMO

Stimulating immunogenic cell death (ICD) is the key to tumor immunotherapy. However, traditional chemoradiotherapy has limited effect on stimulating immunity and often requires repeated administration, which greatly reduces the tumor-killing effect. In this article, we created a sodium alginate hydrogel sustained-release system containing low-dose doxorubicin (Dox) and immune adjuvant R837, which were injected into the interstitial space to wrap around the tumor in situ, achieving a sustained release and long-lasting immune response. Cooperating with immune checkpoint blockade, Dox induced ICD, activated dendritic cells (DCs) and converted immunosuppressive M2-type tumor-associated macrophages (TAM) to tumor-killing M1-type TAMs. Simultaneously, it greatly promoted T cell proliferation and infiltration, and reduced tumor immunosuppressive factors, triggering a robust immune response to suppress tumors in vivo. In conclusion, this anti-tumor strategy based on interstitial injection can achieve continuous local immune stimulation by low-dose chemotherapy drugs, providing a potential approach for tumor immunotherapy.

18.
Ultrasound Med Biol ; 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36456377

RESUMO

Levodopa-induced dyskinesia (LID) is a common motor complication in Parkinson disease (PD). Abnormal substantia nigra hyperechogenicity (SN+), detected by transcranial sonography (TCS), plays an important role in the differential diagnosis of PD. The purpose of this study was to investigate the predictive performance of quantitative SN+ evaluations for LID. Five hundred sixty-two individuals were included in our analysis, and 198 individuals were followed up. These individuals were divided into two groups at baseline: the PD with LID (PD+LID) group and the PD without LID (PD-LID) group. The association between total hyperechogenic area of the SN on both sides (SNT) and LID was analyzed by binary logistic analysis. A binary logistic regression model including SNT was applied to establish a model for discriminating LID. At baseline, 105 (18.7%) individuals were diagnosed with LID. The PD+LID group had a longer disease duration, shorter education duration, higher levodopa equivalent doses, greater disease severity and larger SNT. A model combining clinical features and SNT was further established with better efficiency (area under the receiver operating characteristic curve = 0.839). One hundred ninety-eight individuals were followed up; individuals with a larger SNT and a higher predicted probability were more likely to develop LID in our follow-up. Our study determined that quantitative TCS evaluation of SN echogenicity is useful in predicting LID in PD.

19.
Hypertension ; 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36330812

RESUMO

BACKGROUND: We report the creation and evaluation of a de novo assembly of the genome of the spontaneously hypertensive rat, the most widely used model of human cardiovascular disease. METHODS: The genome is assembled from long read sequencing (PacBio HiFi and CLR) and scaffolded with long-range structural information obtained from Bionano optical maps and proximity ligation sequencing proximity analysis of the genome. The genome assembly was polished with Illumina short reads. Completeness of the assembly was investigated using Benchmarking Universal Single Copy Orthologs analysis. The genome assembly was also evaluated with the rat reference gene set, using NCBI automated protocols. We also generated orthogonal single molecule transcript sequence reads (Iso-Seq) from 8 tissues and used them to validate the coding assembly, to annotate the assembly with RNA transcripts representing unique full length transcript isoforms for each gene and to determine whether divergences between RefSeq sequences and the assembly were attributable to assembly errors or polymorphisms. RESULTS: The assembly analysis indicates that this assembly is comparable in contiguity and completeness to the current rat reference assembly, while the use of HiFi sequencing yields an assembly that is more correct at the single base level. Synteny analysis was performed to uncover the extent of synteny and the presence and distribution of chromosomal rearrangements between the reference and this assembly. CONCLUSION: The resulting genome assembly is reference quality and captures significant structural variation.

20.
J Virol ; : e0157822, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36448809

RESUMO

Cyclic GMP-AMP synthase (cGAS), a key DNA sensor, detects cytosolic viral DNA and activates the adaptor protein stimulator of interferon genes (STING) to initiate interferon (IFN) production and host innate antiviral responses. Duck enteritis virus (DEV) is a duck alphaherpesvirus that causes an acute and contagious disease with high mortality in waterfowl. In the present study, we found that DEV inhibits host innate immune responses during the late phase of viral infection. Furthermore, we screened DEV proteins for their ability to inhibit the cGAS-STING DNA-sensing pathway and identified multiple viral proteins, including UL41, US3, UL28, UL53, and UL24, which block IFN-ß activation through this pathway. The DEV tegument protein UL41, which exhibited the strongest inhibitory effect, selectively downregulated the expression of interferon regulatory factor 7 (IRF7) by reducing its mRNA accumulation, thereby inhibiting the DNA-sensing pathway. Ectopic expression of UL41 markedly reduced viral DNA-triggered IFN-ß production and promoted viral replication, whereas deficiency of UL41 in the context of DEV infection increased the IFN-ß response to DEV and suppressed viral replication. In addition, ectopic expression of IRF7 inhibited the replication of the UL41-deficient virus, whereas IRF7 knockdown facilitated its replication. This study is the first report identifying multiple viral proteins encoded by a duck DNA virus, which inhibit the cGAS-STING DNA-sensing pathway. These findings expand our knowledge of DNA sensing in ducks and reveal a mechanism through which DEV antagonizes the host innate immune response. IMPORTANCE Duck enteritis virus (DEV) is a duck alphaherpesvirus that causes an acute and contagious disease with high mortality, resulting in substantial economic losses in the commercial waterfowl industry. The evasion of DNA-sensing pathway-mediated antiviral innate immunity is essential for the persistent infection and replication of many DNA viruses. However, the mechanisms used by DEV to modulate the DNA-sensing pathway remain poorly understood. In the present study, we found that DEV encodes multiple viral proteins to inhibit the cGAS-STING DNA-sensing pathway. The DEV tegument protein UL41 selectively diminished the accumulation of interferon regulatory factor 7 (IRF7) mRNA, thereby inhibiting the DNA-sensing pathway. Loss of UL41 potently enhanced the IFN-ß response to DEV and impaired viral replication in ducks. These findings provide insights into the host-virus interaction during DEV infection and help develop new live attenuated vaccines against DEV.

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