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1.
Food Chem ; 303: 125400, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31470275

RESUMO

Siraitia grosvenorii fruit (SGF) has been used as a natural sweetener and traditional medicine in China for more than two centuries. This study evaluated the effect of SGF extract supplementation (0.5%, 1%, and 2%) on the chemical, microbial and sensory properties of probiotic yogurt. The antioxidant, angiotensin-converting-enzyme inhibitory (ACE-I) and antibacterial bioactivities were determined. SGF extract supplementation improved some of the chemical and physicochemical characteristics. Probiotic yogurt with the fruit extract had significantly more Lactobacillus casei and Lactobacillus bulgaricus, whereas there was no significant effect on the number of Streptococcus thermophiles. The bioactivities were significantly increased by SGF extract supplementation. Probiotic yogurt with 2% SGF extract showed the highest antioxidant, ACE-I, and antibacterial activities, whereas the one with 1% SGF extract conferred the highest sensory attributes score. Overall, SGF extract offers a promising option as a dietary supplement to produce novel dairy products that have high nutritional and bioactivity values.

2.
J Cell Physiol ; 235(1): 221-231, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31187497

RESUMO

The motility of mesenchymal stem cells (MSCs) is highly related to their homing in vivo, a critical issue in regenerative medicine. Our previous study indicated copper (Cu) might promote the recruitment of endogenous MSCs in canine esophagus defect model. In this study, we investigated the effect of Cu on the motility of bone marrow mesenchymal stem cells (BMSCs) and the underlying mechanism in vitro. Cu supplementation could enhance the motility of BMSCs, and upregulate the expression of hypoxia-inducible factor 1α (Hif1α) at the protein level, and upregulate the expression of rho family GTPase 3 (Rnd3) at messenger RNA and protein level. When Hif1α was silenced by small interfering RNA (siRNA), Cu-induced Rnd3 upregulation was blocked. When Rnd3 was silenced by siRNA, the motility of BMSCs was decreased with or without Cu supplementation, and Cu-induced cytoskeleton remodeling was neutralized. Furthermore, overexpression of Rnd3 also increased the motility of BMSCs and induced cytoskeleton remodeling. Overall, our results demonstrated that Cu enhanced BMSCs migration through, at least in part, cytoskeleton remodeling via Hif1α-dependent upregulation of Rnd3. This study provided an insight into the mechanism of the effect of Cu on the motility of BMSCs, and a theoretical foundation of applying Cu to improve the recruitment of BMSCs in tissue engineering and cytotherapy.

3.
J Cell Physiol ; 235(1): 232-244, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31190343

RESUMO

Diabetes is often accompanied by dysfunction of salivary glands. However, the molecular mechanism remains unclear. The mechanisms that underlie diabetic hyposalivation were studied by db/db mice and SMG-C6 cells. We found morphological changes and decreased stimulated salivary flow rates of the submandibular gland (SMG) in diabetic mice. We observed structural changes and dysfunction of mitochondria. More mitophagosomes and higher expression of autophagy-related proteins were detected. Increased levels of proteins PINK1 and Parkin indicate that PINK1/Parkin-mediated mitophagy was activated in diabetic SMG. Consistently, high glucose (HG) induced mitochondrial dysfunction and PINK1/Parkin-mediated mitophagy in cultivated SMG-C6 cells. HG also increased reactive oxygen species (ROS) and lessened activation of antioxidants in SMG-C6 cells. In addition, HG lowered ERK1/2 phosphorylation and HG-induced mitophagy was decreased after ERK1/2 was activated by LM22B-10. Altogether, these data suggest that ROS played a crucial role in diabetes-induced mitochondrial dysfunction and PINK1/Parkin-mediated mitophagy and ERK1/2 was required in HG-induced mitophagy in SMG.

4.
Nanoscale ; 11(39): 18238-18245, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31565714

RESUMO

Designing low-cost and high-active bifunctional catalysts for overall water splitting has attracted increasing research interest. Herein, the brilliant overall water splitting performance of cobalt-vanadium bimetal-based nanocomposites is explored. Co-V based nanocomposites are synthesized through a one-step hydrothermal method, in which the cobalt species is introduced into the lepidocrocite VOOH and further cobalt vanadium oxide is formed. The additive level of cobalt is optimized and the corresponding effect on electrocatalytic activity is also investigated in this work, systematically. The targeted catalyst (denoted as Co0.2-VOOH) exhibits a unique sheet-like morphology, resulting in the high exposure of catalytically active sites. When used as the bifunctional catalyst, Co0.2-VOOH can achieve a current density of 10 mA cm-2 at the overpotentials of 210 mV for water oxidation and 130 mV for hydrogen generation, respectively. Notably, it only requires low cell voltages of 1.57 and 1.74 V to drive the catalytic current densities of 10 and 100 mA cm-2 during the water splitting process. This work significantly indicates that cobalt-vanadium based materials are promising alternatives for overall water splitting.

5.
Dent Mater J ; 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31582598

RESUMO

To explore the effects of infiltration resin, casein phosphate polypeptide-amorphous calcium phosphate (CPP-ACP) and fluoride on microhardness and micromorphology of irradiated enamel. Sixty human permanent teeth were mesiodistally sectioned, yielding 120 enamel samples, which randomly divided into 8 groups: G1: blank control; G2: irradiation control; G3: irradiation+fluoride; G4: irradiation+CPP-ACP; G5: irradiation+CPP-ACP+fluoride; G6: irradiation+infiltration resin; G7: irradiation+infiltration resin+fluoride; G8: irradiation+infiltration resin+CPP-ACP. A progressive improvement was observed on the superficial morphology of enamel treated with different anti-caries procedures. The order of microhardness values after irradiation from the highest to the lowest was as follows: G1>G8~G7>G6>G5>G4~G3>G2. CPP-ACP, infiltration resin and fluoride can effectively restore the direct destruction of enamel caused by irradiation and promote the occurrence of remineralization. Infiltration resin and its combined effects with fluoride or CPP-ACP have the most potential anti-caries agent to resist radiation-caries.

6.
Ear Hear ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31584503

RESUMO

OBJECTIVES: To gain insight into the current practice of hearing protection of Chinese workers and the value of hearing protection device (HPD) fit testing. DESIGN: HPD fit testing was conducted on workers (N = 774) in a petrochemical plant in Eastern China who were on duty during the period of this study. The 3M E-A-Rfit Dual-Ear Validation System was used to measure the personal attenuation ratings (PARs) of a premolded earplug used at the work site. Repeated fit testing was conducted at approximately 6- or 12-month intervals. Wilcoxon signed rank tests were conducted to analyze the pairwise differences between the baseline, postintervention, and follow-up visit PARs, and Mann-Whitney tests were used to compare the PARs obtained by two follow-up groups. RESULTS: The median PAR baseline was 11 dB; significant improvement was shown on the postintervention PARs (p < 0. 001). No significant difference was shown between PARs obtained during the 6- and 12-month follow-up visits (p > 0.05). Comparing PARs of follow-up visits with the baseline PAR demonstrated a significant improvement (p < 0. 001), but revealed a significant decline (p < 0. 001) comparing with the postintervention PARs. CONCLUSIONS: HPD fit testing showed value added as to verify the sufficiency of attenuation. The training along with fit testing showed contributions to improve PARs, maintained effectiveness over time, and assisted in HPD selection. Follow-up is believed to be important to ensure that the HPDs are continually used correctly. There was no significant difference in the sustained effectiveness of the follow-up when observe 6- and 12-month subsequent to intervention.

7.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3399-3405, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602901

RESUMO

Tripterygium wilfordii is widely used in the treatment of rheumatism with curative effect. However,its toxicity and adverse reactions,especially the hepatotoxicity,rank the first in the herbs induced liver injury,is the key factors hindering its clinical application. This paper reviewed the literatures related to the hepatotoxicity of T. wilfordii in recent 20 years,and summarized the characteristic of hepatotoxicity induced by T. wilfordii,the factors causing liver injury,the mechanism of toxicity,and the measures to reduce toxicity. In animal experiments,the T. wilfordii induced-hepatotoxicity in physiological state was more serious than pathological state. The T. wilfordii induced-hepatotoxicity is related to various toxic components contained in it,but alkaloids are the most toxic one.Overdose and cumulative overdose are the lead causing of hepatotoxicity induced by T. wilfordii. The theory of oxidative stress is still an important mechanism of T. wilfordii induced-hepatotoxicity,and Nrf2,as a key regulatory enzyme of oxidative stress,has become an important target for drugs to against T. wilfordii induced-hepatotoxicity. Mitochondrial autophagy and liver hypersensitivity are new mechanisms of liver injury induced by T. wilfordii. The measures such as dosage control,drug compatibility and dosage form variations can help to reduce the hepatotoxicity induced by T. wilfordii. This paper clarified the current situation and shortcomings of safety research on T. wilfordii,so as to propose new research strategies and provide ideas for rational evaluation of safety and clinical safe drug use of T. wilfordii.

8.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3441-3447, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602907

RESUMO

To observe the effect of Tripterygium Glycosides Tablets on angiogenesis of rats with type Ⅱ collagen-induced arthritis( CIA) and on the tube formation of human umbilical vein endothelial cells( HUVEC) in vitro. The HUVEC were induced by 20 µg·L-1 vascular endothelial growth factor( VEGF) in vitro,and were treated with 0. 1,1,10 mg·L-1 Tripterygium Glycosides Tablets continuously for 7 hours. The numbers of branches of tube formation were measured. SD rats were immunized to establish CIA. CIA rats were treated with 9,18,36 mg·kg-1·d-1 Tripterygium Glycosides Tablets for 42 days. Histopathological examination( HE) was performed to observe the vascular morphology and vascular density in the synovial membrane of the inflamed joints. Immunohistochemistry and immunofluorescence were performed to observe the expression of platelets-endothelial cell adhesion molecule( CD31) and αsmooth muscle actin( αSMA) in synovial membrane. Immunohistochemistry and Western blot were performed to observe the expression of hypoxia-inducible factors 1α( HIF1α) and angiotensin 1( Ang1) in the synovial tissue. The results showed that the numbers of branches of tube formation of HUVEC induced by VEGF were improved,and declined significantly after treated by Tripterygium Glycosides Tablets. Compared with the normal group,the vascular density,CD31 positive expression,CD31 +/αSMA-immature and total vascular positive expression in the synovial membrane of the model group were significantly increased,and so as HIF1α and Ang1 in the synovium. Tripterygium Glycosides Tablets reduced the synovial vascular density and inhibited the positive expression of CD31,CD31+/αSMA-immature blood vessels and total vascular,but has no effect on CD31+/αSMA+mature blood vessels. Tripterygium Glycosides Tablets also inhibited the expression of HIF1α and Ang1 in synovial membrane of inflammatory joints. Our results demonstrated that Tripterygium Glycosides Tablets could inhibit the angiogenesis of synovial tissue in CIA rats and the tube formation of HUVEC,which is related to the down-regulation of HIF1α/Ang1 signal axis.

9.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3486-3493, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602913

RESUMO

The aim of this paper was to observe the toxic effect of Tripterygium Glycosides Tablets( TG) on the reproductive system of Ⅱ type collagen induced arthritis( CIA) male rats,and to explore the toxic mechanism preliminarily. Fifty SD rats were randomly divided into normal control group( Con),model group( CIA),Tripterygium Glycosides Tablets clinical equivalent dose groups of 1,2,4 times( 9,18,36 mg·kg-1),10 rats in each group,and were given by gavage once a day for 42 days after the first immunization.The organ indexes of uterine and ovarian were calculated on days 21 and 42. Histopathological and morphological changes of uterine and ovarian were observed under optical microscope. The concentration of estradiol( E2),follicle-stimulating hormone( FSH),luteinizing hormone( LH),17α-hydroxylase( CYP17 A1) and cytochrome P450 19 A1( CYP19 A1) in serum were detected by ELISA. Immunohistochemistry was used to observe the expression of Bax and Bcl-2 related proteins in the apoptosis pathway of uterus and ovary. The results showed that compared with the Con group,CIA group could reduce the number of uterine glands( P<0.05),but no significant changes were observed in other groups. Compared with the CIA group,there were no significant changes in the coefficients of uterus and ovary in the Tripterygium Glycosides Tablets groups. The number of uterine glands,total follicles in the ovary,mature follicles and corpus luteum,the distribution of blood vessels and mitochondria had a certain inhibitory trend,and also slightly increased the number of atresia follicles,but the histopathological quantitative indicators were not statistically different. Except that 2 times clinical dose of Tripterygium Glycosides Tablets could significantly reduce the content of CYP19 A1( P<0. 05) after 42 d administration,there were no significant changes in serum estrogen E2,FSH,LH and estrogen synthesis key enzymes CYP17 A1 in each administration group. Medium and high doses of Tripterygium Glycosides Tablets could increase the expression of apoptotic protein Bax in uterine and ovarian tissues( P<0. 05,P<0. 01),and all the administration groups could inhibit the expression of apoptotic inhibiting protein Bcl-2( P <0. 05,P<0. 01,P<0.001),42 d was more obvious than 21 d. In conclusion,4 times and less than 4 times Tripterygium Glycosides Tablets did not cause obvious toxicity and histopathological changes in the reproductive organs of CIA rats,but it could reduce the level of serum estrogen synthesis key enzyme CYP19 A1 and affect the content of apoptosis-related proteins Bax and Bcl-2 in uterus and ovary tissues. The relevant mechanism needs further study.

10.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3502-3511, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602915

RESUMO

The aim of this paper was to compare the properties of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets from dose-effect-toxicity on type Ⅱ collagen-induced arthritis( CIA) in rats. SD rats were randomly divided into eight groups,including normal group,model group,Tripterygium Glycosides Tablets groups( 1 times equivalent dose 0.009 g·kg-1,4 times equivalent dose 0.036 g·kg-1,16 times equivalent dose 0.144 g·kg-1),Tripterygium wilfordii Tablets groups( 1 times equivalent dose 0.007 5 mg·kg-1,4 times equivalent dose 0.030 mg·kg-1,16 times equivalent dose 0.120 mg·kg-1). Beginning on the first immunization,Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets administered intraperitoneally once a day. After the second immunization,the symptoms such as redness and swelling of joints were observed,and the clinical score and incidence of arthritis were evaluated. HE and Masson staining were used to examine the histopathological changes of joints. The expression level of anti-type Ⅱ collagen antibody Ig G in serum was detected by ELISA,routine testing of blood components,the concentration of ALP( alkaline phosphatase),ALT( alanine aminotransferase),AST( aspartate aminotransferase),GGT( gamma-glutamyltransferase),TBi L( total bilirubin),CRE( creatinine) and UREA( urea) in serum were detected by enzymatic assay. The rate of sperm deformity in the epididymis was evaluated under light microscope. The extent of damage to the testis and ovarian tissue was assessed by HE staining. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets attenuated the inflammation,redness,swelling and deformity of joints and reduced the clinical score and incidence of arthritis in CIA rats. Meanwhile,it also exhibited obvious reduction in all pathological features such as joint synovitis,pannus,cartilage erosion and bone destruction. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets reduced Ig G in a dose-dependent manner,and Tripterygium Glycosides Tablets is better than Tripterygium wilfordii Tablets( P<0.05 or P<0.01). The high doses of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could significantly increase the organ coefficient of liver and spleen and reduced RBC and HGB in CIA rats( P<0.01),and severity leading to death. Gastric mucosal injury and morphological changes of liver and kidney were not observed in CIA rats of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets treatment group. The 4 and 16 times doses of Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could significantly increase serum ALT,GGT and decrease CRE( P<0.05 or P<0.01). Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets could increase the sperm deformity rate and damage the testicular seminiferous tubules of CIA male rats. Severity increased with dose and time increasing. The effect of Tripterygium Glycosides Tablets( 16 times) is more significant than Tripterygium wilfordii Tablets( 16 times). Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets significantly delayed onset of arthritis and inhibited the paw edema and arthritic score. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets also caused male reproductive damage,high dose affected hematopoiesis,and maximum dose leading to death. Tripterygium Glycosides Tablets and Tripterygium wilfordii Tablets all depended on dose-effect-toxicity manner. Anti-arthritis effect of Tripterygium Glycosides Tablets is better than Tripterygium wilfordii Tablets,but the toxicity of Tripterygium Glycosides Tablets maximum dose is more obvious. The relevant conclusions of our study will provide experimental references for clinical rational use of drugs,and further clinical studies are needed to confirm our conclusions.

11.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(10): 989-992, 2019 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-31598942

RESUMO

OBJECTIVE: To explore the nature and origin of chromosomal copy number variants (CNVs) in a pedigree affected with mental retardation. METHODS: Genomic CNVs of the proband were analyzed by next generation sequencing (NGS). Chromosomal karyotypes of the proband and his relatives were analyzed with high-resolution karyotyping and fluorescence in situ hybridization (FISH). RESULTS: Clinical phenotypes of the proband and other patients from the pedigree included mental retardation and mild dysmorphism. The results of NGS revealed that the proband carried a 16.24 Mb microduplication at 4p16.3-15.32 and a 2.2 Mb microdeletion at 8p23.3-23.2. Other patients of the pedigree harbored the same variants, while those without the phenotypes did not harbor the variants. The results of high-resolution karyotyping and FISH revealed that the mother of the proband carried a reciprocal translocation between 4p and 8p, and her karyotype was 46,XX,t(4;8)(p16;p23). No karyotypic abnormality was detected in his father. CONCLUSION: The abnormal phenotypes of this pedigree may be attributed to 4p microduplication in conjunct with 8p microdeletion derived from a maternal balanced translocation between 4p and 8p.

13.
Int J Gynecol Pathol ; 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31569185

RESUMO

Endometrial stromal sarcoma (ESS) is a rare malignant tumor of the uterus that has been described as the second most common malignant uterine mesenchymal tumor. The Primary extrauterine ESS (EESS) is an extremely uncommon occurrence. We hereby report a new bona fide case of low-grade EESS in a 74-yr-old woman arising in the vagina, presenting as a polypoid mass associated with irregular vaginal bleeding. On examination, a 6×2×2 cm polypoid mass was found in the left vaginal wall. Consequently, the patient underwent partial vaginectomy and repair. No ESS or endometriotic lesion was found in the endometrium and bilateral adnexa. The diagnosis of ESS performed by typical pathologic and immunohistochemical evaluation was as follows: beta-catenin (+++), estrogen receptor (+++), progesterone receptor (++), vimentin (++), and uniformly negative for CD10, EMA, CD31, CD34, CD117,CD99, SMA, desmin, h-caldesmon, S-100, MelanA, and HMB45. She has remained disease free with no signs or symptoms of recurrent or advanced disease for 46 mo. Although CD10 is the most useful immunohistochemical marker for the diagnosis of this tumor, negative CD10 staining can be encountered with underfixation. Therefore, it is important to use a panel of immunostains that includes CD10, beta-catenin, and smooth muscle markers. The present study describes the clinical and pathologic features of low-grade EESS through a case report and literature review. To the best of our knowledge, this is the eighth report of EESS arising from the vagina.

14.
J Mater Chem B ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31591619

RESUMO

Multifunctional drug carriers for simultaneous imaging and drug delivery have emerged as an important new direction for the treatment of cancer. In this study, 3d-4f heterometallic Fe/La-MOFs, with excellent fluorescence and superior positive magnetic resonance imaging with high resolution, were synthesized successfully. The feasibility of Fe/La-MOFs for use in multifunctional drug delivery was demonstrated using doxorubicin hydrochloride (DOX) as the drug model. After modification with tunable amino modified silica layers, the drug loading increased to 150.2 mg g-1 from 23.9 mg g-1, and the pH responsive drug release climbed 80% from 10% upon regulating the pH from 5.8 to 7.4. T2-Weighted magnetic resonance imaging (MRI) and fluorescence optical imaging (FOI) both showed a clear concentration-dependent contrast enhancement, indicating potential application as a MRI/FOI dual mode imaging agent. In addition, the FOI of 4T1 cells loaded with Fe/La-MOFs demonstrated their capacity for imaging living cells. The particles were tracked by MRI, and the transverse relativity (r2) ranks among the highest reported for Fe3+complexes (100.49 mM-1 s-1). In summary, this is the first report on 3d-4f MOF particles displaying pH-responsive delivery and MRI/FOI dual mode imaging.

15.
Aging (Albany NY) ; 112019 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-31586991

RESUMO

PURPOSE: The aim of this study was to determine the impact of analyzing age as a continuous variable on survival outcomes and treatment selection for extranodal nasal-type NK/T-cell lymphoma. RESULTS: The risk of mortality increased with increasing age, without an apparent cutoff point. Patients' age, as a continuous variable, was independently associated with overall survival after adjustment for covariates. Older early-stage patients were more likely to receive radiotherapy only whereas young-adult advanced-stage patients tended to receive non-anthracycline-based chemotherapy. A decreased risk of mortality with radiotherapy versus chemotherapy only in early-stage patients (HR, 0.347, P < 0.001) or non-anthracycline-based versus anthracycline-based chemotherapy in early-stage (HR, 0.690, P = 0.001) and advanced-stage patients (HR, 0.678, P = 0.045) was maintained in patients of all ages. CONCLUSIONS: These findings support making treatment decisions based on disease-related risk factors rather than dichotomized chronological age. PATIENTS AND METHODS: Data on 2640 patients with extranodal nasal-type NK/T-cell lymphoma from the China Lymphoma Collaborative Group database were analyzed retrospectively. Age as a continuous variable was entered into the Cox regression model using penalized spline analysis to determine the association of age with overall survival (OS) and treatment benefits.

16.
Elife ; 82019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478829

RESUMO

Prostate is the most frequent cancer in men. Prostate cancer progression is driven by androgen steroid hormones, and delayed by androgen deprivation therapy (ADT). Androgens control transcription by stimulating androgen receptor (AR) activity, yet also control pre-mRNA splicing through less clear mechanisms. Here we find androgens regulate splicing through AR-mediated transcriptional control of the epithelial-specific splicing regulator ESRP2. Both ESRP2 and its close paralog ESRP1 are highly expressed in primary prostate cancer. Androgen stimulation induces splicing switches in many endogenous ESRP2-controlled mRNA isoforms, including splicing switches correlating with disease progression. ESRP2 expression in clinical prostate cancer is repressed by ADT, which may thus inadvertently dampen epithelial splice programmes. Supporting this, treatment with the AR antagonist bicalutamide (Casodex) induced mesenchymal splicing patterns of genes including FLNB and CTNND1. Our data reveals a new mechanism of splicing control in prostate cancer with important implications for disease progression.

17.
Gynecol Endocrinol ; : 1-5, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478751

RESUMO

Purpose: With the wide implementation of the universal two-child policy in China, the number of pregnant women in advanced maternal age (AMA) will increase gradually. We aimed to assess the association between age at menarche (AAM) and insulin resistance (IR) before delivery in AMA. Methods: A total of 80 pregnant women in AMA were consecutively enrolled before delivery in Zhongda hospital. Pregnant women were stratified into early menarche group and late menarche group according to the age of regular menstruation (about 13 years). At delivery, serum glucose and lipid levels were measured. IR was calculated by the method of homeostasis model assessment 2(HOMA2). Results: The fasting blood insulin (17.68(9.72-36.71) and 10.35(7.76-15.10), respectively; p = .006) and HOMA-IR (2.08(1.18-4.37) and 1.24(0.89-1.78), respectively; p = .005) were higher in early menarche group than in late menarche group. AAM was inversely associated with HOMA-IR in AMA (r= -0.27, p = .014). In the multivariable analysis, AAM in late menarche group was negatively related to the level of HOMA-IR compared to those in early menarche group (ß= -2.275, p≤.0001). Conclusions: Taken together, our findings suggest that AAM was inversely associated with HOMA-IR in AMA. Furthermore, pregnant women in AMA with early menarche might have higher HOMA-IR levels than those with late menarche. Clinical trial registration: Chinese Clinical Trial Registry (No. ChiCTR-RRC-16008714), retrospectively registered.

18.
Mikrochim Acta ; 186(10): 675, 2019 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-31494752

RESUMO

Nitrogen-doped carbon dots (N-CDs) with fluorescence excitation/emission maxima at 365/450 nm were prepared by a one-step hydrothermal approach. The dots possess remarkable photostability, fluorescence blinking and good biocompatibility, and this favors utilization in stochastic optical reconstruction microscopy (STORM). A spatial resolution down to ~60 nm was achieved when imaging HeLa cells using 647-nm laser excitation. This opens new possibilities for various super-resolution techniques based on stochastic optical switching. The remarkable optical properties of the N-CDs also enable them to be applied as invisible security ink for use in patterning, information storage and anti-counterfeiting. Further, it is found that the fluorescence of the N-CDs can be quenched by curcumin with high efficiency due to a combination of inner filter effect and static quenching. Based on this, a fluorometric assay with a detection limit of 21 ng mL-1 was developed for the determination of curcumin. Graphical abstract Schematic representation of the applications of N-doped carbon dots (N-CDs). Curcumin quenches the fluorescence of N-CDs with high efficiency. The remarkable optical properties of the N-CDs enable them to be applied in fluorescent ink, cell imaging and stochastic optical reconstruction microscopy (STORM).

19.
Opt Express ; 27(17): 24693-24704, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31510354

RESUMO

We investigate the effects of geometrical and structural disorders on perfectly asymmetric diffraction (PAD) in Raman-Nath regime. The two types of disorders are realized by introducing random fluctuations in the position and width of one-dimensional (1D) driven atomic lattices. Raman-Nath diffraction is modified differently with respect to the geometrical and structural disorders. It is shown that the PAD is observed with a certain strength range of geometrical disorder, exceeding which it can be destroyed, while the PAD is rather robust against structural disorder. The different behaviors originate from the disorder-induced random variations of the spatial phase shifts of the standing-wave (SW) coupling field and atomic lattices with Gaussian profile. Furthermore, we find that, in the presence of geometrical disorder, the PAD is more susceptible to correlated disorder than to uncorrelated disorder. Our scheme may be useful for understanding the effects of disorder on the diffraction of light and matter waves in disordered potentials..

20.
Artigo em Inglês | MEDLINE | ID: mdl-31512124

RESUMO

The activation of persulfate to produce active radicals has been attracting wide attention in environmental remediation fields. Among various catalysts, non-metal carbocatalysts and carbon-based composites have shown attractive prospects given that they are environmental-friendly, highly efficient, abundant, and diverse. In this paper, the use of carbon-based magnetic nanocomposites as catalysts for persulfate activation was reviewed and discussed. The preparation methods of carbon-based magnetic nanocomposites were first briefly summarized. Subsequently, the use of activated carbon, carbon nanotubes, graphene oxide, biochar, and nanodiamond-based magnetic composites to activate persulfate was discussed, respectively. A synergetic effect between carbon materials and magnetic nanoparticles facilitated the activation process because of the increased electron transfer capacity, good dispersity of magnetic nanoparticles, and good repeatability and separability. Both radical and non-radical pathways were detected in the activation processes, but the specific mechanisms were greatly influenced by the components of the catalyst and solution conditions. And fundamental studies were needed to clarify the inner mechanisms of the process. In the end, strategies for enhancing the catalytic performances of carbon-based magnetic nanocomposites were suggested. It is expected that this review will provide some inspirations for developing highly efficient and green catalyst, as well as sulfate radical-based advanced oxidation technology for the remediation water environment.

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