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1.
Carbohydr Polym ; 300: 120273, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372495

RESUMO

The genotype of rice variety is important factor that affect grain quality during storage. In this study, japonica, indica, and indica-japonica hybrid rice were selected to reveal the changes in amylase activities, starch fine structure, and physicochemical properties after one year of storage. Amylase activities decreased after storage and starch molecules were degraded, which, respectively, decreased and increased the content of the amylopectin long chains (DP 37+) and amylose. Changes in the starch fine structure resulted in lower swelling factors and a higher gelatinization temperature. Compared with japonica and indica-japonica hybrid rice, indica rice had a more stable starch fine structure and physicochemical properties after storage. The information obtained from this study provides a better understanding of the changes that occur in starch at the molecular level during storage and will provide insight into the breeding of storable rice varieties.


Assuntos
Oryza , Amido , Amido/química , Oryza/química , Amilopectina/química , Amilose/química , Amilases/metabolismo
2.
J Environ Sci (China) ; 125: 678-690, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36375949

RESUMO

Antibiotics in soil environment are regarded as emerging pollutants and have introduced increasing risks to soil ecosystem and human health in rapid urbanization areas. Identifying the occurrence and spatial variability of antibiotics in soils is an urgent issue in sustaining soil security. In this study, antibiotics in soils were investigated and analyzed in Beijing-Tianjin-Hebei urban agglomeration. The occurrence, spatial distribution, and related affecting factors of antibiotics in soils were identified and ecological risks of antibiotics in soil environment were assessed. Results showed that (1) The mean concentration of soil antibiotics in Beijing-Tianjin-Hebei urban agglomeration was 21.79 µg/kg. Land use substantially affected the occurrence and concentration of antibiotics in soils. Concentrations of antibiotics in cropland and orchard soils were 2-3 times higher than the other land use types. (2) The concentrations of antibiotics in soils in Beijing-Tianjin-Hebei urban agglomeration presented a spatial pattern of high values in southeast, and low values in northwest. Spatial variability of antibiotics in soils was closely related to the application of organic fertilizer and wastewater irrigation as well as topographical features. Furthermore, soil properties and land management policy had substantial influences on soil antibiotics, and soil heavy metals may aggravate the accumulation of antibiotics in soils. (3) Ecological risks assessment of antibiotics in soils demonstrated that erythromycin (ERY), sulfamethoxazole (SMX), and doxycycline (DOX) may introduce high risks to soil ecosystem health, and more attention should be paid to the areas with intensive human activities that had potential high risk to soil ecosystem health. This study suggests that scientific land and soil management should be considered to prevent soil antibiotic pollution and sustain soil security in urban agglomeration.


Assuntos
Metais Pesados , Poluentes do Solo , Humanos , Solo , Poluentes do Solo/análise , Antibacterianos/análise , Ecossistema , Metais Pesados/análise , Águas Residuárias , China , Monitoramento Ambiental , Medição de Risco
3.
Talanta ; 252: 123825, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35985190

RESUMO

A fluorescence and photothermal dual-signal assay was developed for the determination of ascorbic acid (AA). Cerium/polyacrylic acid modified porphyrin metal-organic frameworks (PCN-224@PAA-Ce MOFs) exhibit oxidase-like activity and can effectively oxidize 3,3 ', 5,5 '-tetramethylbenzidine (TMB) from colorless substrates to blue substrates (oxTMB). The absorption spectrum of oxTMB overlaps with the emission spectrum of PCN-224@PAA-Ce, resulting in quenching of fluorescence emission of PCN-224@PAA-Ce. oxTMB, as an excellent photothermal agent, converts the optical signal into the thermal signal (temperature increasing) driven by 808 nm laser. In the presence of AA, the blue oxTMB was reduced to colorless TMB, which led to the fluorescence recovery of PCN-224@PAA-Ce and at the same time, the photothermal signal was changed with the temperature decreasing under the driving of 808 nm laser. The detection limits (LOD) of fluorescence/photothermal dual-mode AA sensor strategies were as low as 0.73 µM and 1.4 µM, respectively. Rapid analysis of ascorbic acid was accomplished with the present procedure, AA in vitamin C tablets and human serum samples were validated.


Assuntos
Cério , Estruturas Metalorgânicas , Porfirinas , Humanos , Colorimetria/métodos , Carbonitrila de Pregnenolona , Ácido Ascórbico/análise , Limite de Detecção
4.
J Hazard Mater ; 441: 129945, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36113345

RESUMO

Given the large amount of toxic Cr(VI) wastewater from various industries, it is urgent to take effective treatment measures. Adsorption has been regarded as highly desirable for Cr(VI) removal, but the effectiveness of most adsorbents is significantly dependent on pH value, in which precipitous performance drop and even structural collapse generally occur in strong acidic/alkaline aqueous. Thus, maintaining high adsorption performance and structural integrity over a wide pH range is challenging. To efficiently remove Cr(VI), we designed and prepared of an acid-base resistant metal-organic framework (MOF) Zr-BDPO, by introducing weak acid-base groups (-NH-, -N= and -OH) onto the ligand. Zr-BDPO achieved a maximum adsorption capacity of 555.6 mg·g-1 and retained skeletal structure at pH= 1-11. Interestingly, all these groups can generate conjugate acid-base pairs by means of H+ and OH- in the external solution and then form buffer layer. The removal of Cr(VI) at a broad range of pH values primarily via hydrogen bonds between -NH- and -OH, and the oxoanion species of Cr(VI) is unusual. This strategy that insulating high concentrations of acids and bases and relying on hydrogen bonds to capture Cr(VI) oxoanions provides a new perspective for actual Cr(VI) wastewater treatment.


Assuntos
Estruturas Metalorgânicas , Poluentes Químicos da Água , Adsorção , Pareamento de Bases , Cromo/química , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Cinética , Ligantes , Águas Residuárias , Poluentes Químicos da Água/química
5.
J Hazard Mater ; 441: 129953, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36116313

RESUMO

The neurotoxin ß-N-methylamino-L-alanine (BMAA) has been presumed as an environmental cause of human neurodegenerative disorders, such as Alzheimer's disease. Marine diatoms Thalassiosira minima are demonstrated here to produce BMAA-containing proteins in axenic culture while the isomer diaminobutyric acid was bacterially produced. In the co-culture with Cyanobacterium aponinum, diatom growth was inhibited but the biosynthesis of BMAA-containing proteins was stimulated up to seven times higher than that of the control group by cell-cell interactions. The stimulation effect was not caused by the cyanobacterial filtrate. Nitrogen deprivation also doubled the BMAA content of T. minima cells. Transcriptome analysis of the diatom in mixed culture revealed that pathways involved in T. minima metabolism and cellular functions were mainly influenced, including KEGG pathways valine and leucine/isoleucine degradation, endocytosis, pantothenate and CoA biosynthesis, and SNARE interactions in vesicular transport. Based on the expression changes of genes related to protein biosynthesis, it was hypothesized that ubiquitination and autophagy suppression, and limited COPII vesicles transport accuracy and efficiency were responsible for biosynthesis of BMAA-containing proteins in T. minima. This study represents a first application of transcriptomics to investigate the biological processes associated with BMAA biosynthesis in diatoms.


Assuntos
Diamino Aminoácidos , Diatomáceas , Diamino Aminoácidos/análise , Coenzima A/metabolismo , Toxinas de Cianobactérias , Diatomáceas/genética , Diatomáceas/metabolismo , Humanos , Isoleucina/metabolismo , Leucina/metabolismo , Neurotoxinas/análise , Nitrogênio/metabolismo , Proteínas SNARE/metabolismo , Espectrometria de Massas em Tandem , Transcriptoma , Valina/metabolismo
6.
Neural Regen Res ; 18(2): 344-349, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35900428

RESUMO

The current animal models of stroke primarily model a single intracerebral hemorrhage (ICH) attack, and there is a lack of a reliable model of recurrent ICH. In this study, we established 16-month-old C57BL/6 male mouse models of ICH by injecting collagenase VII-S into the left striatum. Twenty-one days later, we injected collagenase VII-S into the right striatum to simulate recurrent ICH. Our results showed that mice subjected to bilateral striatal hemorrhage had poorer neurological function at the early stage of hemorrhage, delayed recovery in locomotor function, motor coordination, and movement speed, and more obvious emotional and cognitive dysfunction than mice subjected to unilateral striatal hemorrhage. These findings indicate that mouse models of bilateral striatal hemorrhage can well simulate clinically common recurrent ICH. These models should be used as a novel tool for investigating the pathogenesis and treatment targets of recurrent ICH.

7.
Medicine (Baltimore) ; 101(43): e31633, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36316829

RESUMO

Handrail height and knee joint support both significantly influence sit-to-stand (STS) movement. However, research on the associations between handrail height, knee joint support, and their cumulative effect on STS kinematics and changes in plantar pressure distribution during STS under different handrail heights and knee joint support is still unclear. The main objective of this study was to examine the influence of handrail height and knee joint support on the kinematics and the distribution of plantar pressure in healthy adults during STS. Twenty-six healthy adult subjects aged 23 to 58 years participated in this experiment. The subjects carried out STS movement experiments under 7 conditions: 6 experimental conditions of 3 different heights of handrail, with and without knee joint support, and 1 control condition of standing up naturally. The motions of the markers were recorded using cameras operating at 60 Hz, and total movement time, the percentage of movement time of each phase, trunk tilt angle, joint angle, plantar pressure, and the time from hindfoot to forefoot peak pressure were analyzed and compared. Handrail height significantly influences the percentage of movement time at phase I (P = .015) and the maximum trunk tilt angle (P < .05), knee joint support significantly influences the maximum trunk tilt angle and ankle angle (P = .033), and handrail height and knee joint support have an interaction on the time from hindfoot to forefoot peak pressure (P < .001). Subjects' STS performance was improved with the use of assistant devices but showed particular improvement under the condition of with knee joint support when the handrail height was middle handrail.


Assuntos
Movimento , Postura , Adulto , Humanos , Articulação do Joelho , Fenômenos Biomecânicos , Articulação do Tornozelo
8.
Sheng Li Xue Bao ; 74(5): 737-750, 2022 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-36319097

RESUMO

This study aimed to investigate the effect of microRNA-155 (miR-155) in chronic obstructive pulmonary disease (COPD) and cigarette smoke extract (CSE)-treated airway smooth muscle cells (ASMCs) by targeting phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to regulate the PTEN/PI3K/Akt signaling pathway. The COPD mouse model was induced by lipopolysaccharide (LPS) combined with passive smoking. After modeling, miR-155 mimics and miR-155 inhibitor were used for intervention treatment. The pulmonary function of each group was detected by an EMKA detector. Hematoxylin-eosin (HE) staining was used to observe the pathological changes and scores of lung tissues. The expression of TNF-α, IL-6, and IL-1ß in bronchial alveolar lavage fluid (BALF) was detected by ELISA. Primary ASMCs were isolated and cultured in adherent tissue culture. The proliferation activity was detected by CCK-8 and EdU assays. Transwell and wound healing assays were used to measure the migration of ASMCs. The targeting relationship between miR-155 and PIK3R1 was validated by a double luciferase reporter gene assay. The expression levels of miR-155 and PIK3R1 mRNA in lung tissues of mice in each group were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Western blot was used to detect the expression levels of Ki67, PNCA, PTEN, p-PI3K, PI3K, p85α, p-Akt, and Akt in lung tissues and ASMCs. The results showed that lung function was significantly reduced in the miR-155 mimic group, and the levels of PIK3R1 were significantly increased; while lung function in the miR-155 inhibitor group was significantly improved. The results of HE staining showed that there was obvious inflammatory cell infiltration in the miR-155 mimics group compared to that of the model group. Lung histopathological injury was significantly reduced in the miR-155 inhibitor group, accompanied by decreased expression of Ki67, PNCA, PI3K, p-Akt, increased PTEN and p85α protein levels, and reduced levels of TNF-α, IL-6, and IL-1ß in BALF. The results of the double luciferase reporter gene assay showed that miRNA-155 could target bind to PIK3R1. Compared with those in the CSE+miR-155 NC group, the proliferation and migration of ASMCs were significantly increased in the CSE+miR-155 group. The proliferation and migration of ASMCs were significantly attenuated in the CSE+miR-155+pcDNA PIK3R1 group compared with those in the CSE+miR-155 group, accompanied by decreased expression of Ki67, PNCA, p-Akt and increased PTEN and p85α protein levels. These results suggest that miR-155 activates the PTEN/PI3K/Akt signaling pathway by targeting PIK3R1 to promote the occurrence and development of COPD, which provides new evidence for the use of miR-155 in the treatment of COPD.


Assuntos
MicroRNAs , Doença Pulmonar Obstrutiva Crônica , Animais , Camundongos , Interleucina-6 , Antígeno Ki-67 , Fosfatidilinositol 3-Quinases , Proteínas Proto-Oncogênicas c-akt , Fator de Necrose Tumoral alfa
9.
Soft comput ; : 1-17, 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36320405

RESUMO

This paper proposes an intelligent attendance monitoring system based on spatio-temporal human action recognition, which includes human skeleton gait recognition, multi-action body silhouette recognition and face recognition. Our system solves several problems, for example, when a mask is worn to conceal the face, which leads to a decrease in recognition accuracy performance, and when a 3D face mask is used to fake an identity. The skeleton gait feature of our intelligent attendance monitoring system uses a temporal weighted K-nearest neighbours algorithm to train the recognition model and carry out identification, while the multi-action body silhouette feature uses a multiple K-nearest neighbours algorithm to train the recognition model, identify the person and vote on the outcome. Using the proposed system, which integrates skeleton gait features, action silhouette features and face features, more effective recognition can be achieved. When the system encounters a situation with feature masking, such as when an individual is wearing a mask or has changed their clothes, or when the viewing angle is masked, it can continue to deliver good recognition ability through multi-angle skeleton synthesis gait recognition. Our experimental results show that the recognition accuracy of the system is 83.33% when a specific person wears a mask and passes through a monitored area. The intelligent attendance monitoring system uses a LINE messaging API as the access control notification function and provides a responsive web platform that allows managers to perform follow-up management and monitoring.

10.
Inflamm Res ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329130

RESUMO

BACKGROUND: Rosacea, a chronic inflammatory disorder of the facial skin, is effectively treated by intense pulsed light (IPL). OBJECTIVE: To explore the potential molecular mechanism underlying the photobiomodulation effect of IPL for rosacea treatment. METHODS: Skin samples from patients with rosacea were subjected to histological and immunohistological staining. Ten patients were followed up after IPL treatment using the VISIA® skin analysis system, and the severity was assessed. In vivo, skin changes in mice with rosacea-like inflammation induced by intradermal injection of 320 µM LL-37 with or without IPL treatment were evaluated using L*a*b colorimetry as well as histological and immunological staining. In vitro, LL-37-stimulated mast cells (MCs) with or without IPL treatment were evaluated for protein expression of matrix metalloproteinase (MMP)-9, kallikrein-related peptidase 5 (KLK5), and cathelicidin using western blotting and qRT-PCR. RESULTS: Profound infiltration of inflammatory cells and evident MC degranulation were found in rosacea skin lesions. The expression of rosacea-related biomarkers and inflammatory cytokines was higher in lesional areas than in non-lesional areas, as demonstrated via immunochemical staining. In all patients, rosacea severity reduced after IPL therapy. In vivo, IPL alleviated inflammation in mice with rosacea-like inflammation, as demonstrated by the significantly decreased MMP-9, KLK5, and cathelicidin expression and reduced percentage of degranulating MCs. In vitro, IPL decreased MMP-9, KLK5, and cathelicidin expression in P815 cells, reducing the release of inflammatory cytokines and inhibiting rosacea-like inflammatory reactions. CONCLUSION: The photobiomodulation effect of IPL for rosacea treatment may inhibit MC degranulation and alleviate inflammatory reactions.

11.
Zool Stud ; 61: e19, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36330034

RESUMO

The bark-stripping behavior of Formosan sambar, Rusa unicolor swinhoii, has become conspicuous in recent years in the Tataka area of Yushan National Park in Taiwan and a cause for concern to visitors and ecologists. We conducted a monthly survey of 537 tagged trees of 21 species and monitored the abundance of sambar using camera traps from October 2018 to January 2021, aiming to interpret possible causes of the bark-stripping behavior in Tataka. We also used a generalized linear model to evaluate factors that may affect the probability of a tree having its bark stripped. Both our observations and the model predictions showed that sambar has a strong preference for bark of Pinus armandii, Photinia niitakayamensis, and Salix fulvopubeseens and for trees with diameter at breast height around 14 cm. Bark stripping mainly occurred between July and October when major forage was most abundant. However, sambar's need for bark surged in May when sambar abundance was moderate and decreased in October when sambar abundance was high. The seasonality of bark stripping was synchronized with the peak periods of antler development, fawn nursing, and spread of gastrointestinal parasites, suggesting that sambar strips bark to ingest minerals for their physiological needs and/or to acquire plant secondary metabolites to repel gastrointestinal parasites. Sambar abundance alone was not sufficient to predict the overall intensity of bark stripping. Rather, the product of sambar abundance and the necessity index (average wound size) were strongly correlated with the overall bark-stripping intensity. Therefore, controlling sambar abundance is essential but it alone may not be the optimal strategy for controlling bark stripping. A combination of population control and relaxing of sambar's parasite loading and/or physiological needs for minerals is an important strategy to control the overall bark stripping. Future research could use the necessity index to investigate the synchronicity of the bark-stripping behavior, deer's physiological state, environmental factors and phenology to better understand the cause of this behavior.

12.
Small ; : e2205359, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333111

RESUMO

The rapid progress of information technology is accompanied by plenty of information embezzlement and forgery, but developing advanced encryption technologies to ensure information security remains challenging. Phase separation commonly leads to a dramatic change in the transmittance of hydrophilic polymer networks, which is a potential method for information security but is often neglected. Here, taking the polyacrylamide (PAAm) hydrogel system as a typical example, facilely adjustable information encryption and decryption via its regulable phase separation process in ethanol/water mixed solvent, are reported. By controlling the osmotic pressure of the external and internal environment, it is demonstrated that the diffusion coefficient during deswelling and reswelling, as well as the corresponding change of transmittance of the gel, can be well controlled. Relatively high osmotic pressure leads to rapid phase separation of the initial gel but slow phase remixing of the phase-separated gel, opening the opportunity of applying the gel as a reversible information encryption device. As proof-of-concept demonstrations, several stable and reversible information encryption and decryption systems by making use of the phase separation process of the gels are designed, which are expected to inspire the development of next-generation soft devices for information technology.

13.
Anal Bioanal Chem ; 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333614

RESUMO

Developing simple, efficient, and inexpensive method for trace amount organophosphorus pesticides' (OPs) detection with high sensitivity and specificity is of significant importance for guaranteeing food safety. Herein, an Ag/Au bimetallic nanoparticle-based acetylcholinesterase (AChE) surface-enhanced Raman scattering (SERS) biosensor was constructed for in situ simple and sensitive detection of pesticide residues in food. The principle of this biosensor exploited 4-mercaptophenylboronic acid (4-MPBA)-modified Ag/Au bimetallic nanoprobes as SERS signal probe to improve sensitivity and stability. The combination of AChE and choline oxidase (CHO) can hydrolyze acetylcholine (ATCh) to generate H2O2. The product of H2O2 selectively oxidizes the boronate ester of 4-MPBA, decreasing the Raman intensity of the B-O symmetric stretching. In the presence of OPs, it could inhibit the production of H2O2 by destroying the AChE activity, so the reduction of the SERS signal was also alleviated. Based on the principle, an Ag/Au bimetallic nanoparticle-based AChE SERS sensor was established without any complicated pretreatments. Benefiting from the synergistic effects of Ag/Au bimetallic hybrids, a linear detection range from 5×10-9 to 5×10-4 M was achieved with a limit of detection down to 1.7×10-9 M using parathion-methyl (PM) as the representative model of OPs. Moreover, the SERS biosensor uses readily available reagents and is simple to implement. Importantly, the proposed SERS biosensor was used to quantitatively analyze OP residues in apple peels. The levels of OPs detected in real samples by this method were consistent with those obtained using gas chromatography-mass spectrometry (GC-MS), suggesting the proposed assay has great potential applications for OPs in situ detection in food safety fields.

14.
Sheng Li Xue Bao ; 74(5): 726-736, 2022 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-36319096

RESUMO

The central circadian clock and feeding rhythm coordinately reset peripheral circadian clocks. Emerging evidence suggests that feeding rhythm resets peripheral circadian clocks in a tissue-specific manner. This study aimed to determine whether and how feeding rhythm regulates circadian rhythms of the circadian clock and metabolic genes in brown adipose tissue (BAT). We applied different regimens of time-restricted feeding (TRF) in wildtype and Per1/2 deficient C57BL/6 mice, and quantified the effects of sex, treatment duration, constant light, and circadian clock on circadian rhythms of the BAT circadian clock and metabolic genes by RT-qPCR; Representative circadian clock genes are Bmal1, Nr1d1, Dbp, and Per2, and representative metabolic genes are uncoupling protein 1 (Ucp1), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3) that controls the flux through glycolysis, pyruvate dehydrogenase kinase isozyme 4 (Pdk4) gating the tricarboxylic acid cycle, and carnitine palmitoyltransferase 1A (Cpt1a) that controls mitochondrial fatty acid oxidation. The results showed that, daytime-restricted feeding (DRF) moderately shifted the phase of the BAT circadian clock in female mice within 7 or 36 d, and resulted in the loss of circadian rhythm in Dbp and Per2 transcripts in males. DRF induced de novo oscillation of the Ucp1 transcript, and shifted the phase of representative metabolic genes, such as Pfkfb3, Pdk4, and Cpt1a, more than 7 h. Constant light is known to disrupt the synchrony of the central circadian clock. The results showed that constant light promoted phase entrainment of the circadian clock by DRF in BAT, but abolished the oscillation of the metabolic genes (except for Pdk4). Despite combined treatment with Per1/2 deficiency and constant darkness, DRF was sufficient to drive circadian rhythms of Bmal1 and Dbp, but not those of Nr1d1, Ucp1, Pfkfb3, and Cpt1a. Overall, the circadian clock of BAT has weak adaptation to altered feeding rhythms and sex differences. The central circadian clock antagonizes DRF in the entrainment of the BAT circadian clock, whereas DRF resets circadian rhythms of metabolic genes, such as Ucp1, Pfkfb3, and Cpt1a, in a circadian clock-dependent manner.


Assuntos
Relógios Circadianos , Feminino , Masculino , Animais , Camundongos , Camundongos Endogâmicos C57BL , Tecido Adiposo Marrom , Fatores de Transcrição ARNTL , Ritmo Circadiano
15.
Acta Cardiol ; : 1-10, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36326187

RESUMO

BACKGROUND: Growing studies show that lipoprotein (a) [Lp(a)] is related to calcified aortic valve diseases in general population, while the relationship between Lp(a) and aortic valve calcification (AVC) in patients with new-onset acute myocardial infarction (AMI) remains unclear. Therefore, this study was to evaluate the correlation between Lp(a) and AVC in patients with new-onset AMI. METHODS: This cross-sectional study included 410 patients with new-onset AMI who were hospitalised in Zhongda Hospital affiliated to Southeast University from January 1, 2020 to December 31, 2021. Multivariable logistic regression, subgroup analysis, generalised additive model, threshold and saturation effect and receiver operator characteristic (ROC) curve were used to explore the association between Lp(a) and AVC. RESULTS: Patients with AVC had higher levels of Lp(a) than those without AVC. Multivariable logistic regression analysis showed that higher Lp(a) was still associated with higher risk of AVC after adjusting for confounding factors, and this correlation was robust in most subgroups and sensitivity analyses (p < 0.05). Additionally, the generalised additive model showed that there was a nonlinear correlation between Lp(a) and AVC (P for nonlinearity = 0.037). Threshold and saturation effect analysis indicated that when Lp(a) < 840 mg/L, it was positively correlated with the prevalence of AVC (p < 0.05), but when Lp(a) ≥ 840 mg/L, this correlation no longer existed. Besides, ROC curve analysis demonstrated that Lp(a) had a good diagnostic performance for AVC. CONCLUSION: Lp(a) was independently associated with the prevalence of AVC in patients with new-onset AMI.

16.
Hepatol Int ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36327052

RESUMO

BACKGROUND: Several models have been proposed to predict acute liver failure/death in patients with drug-induced liver injury (DILI), but the predictive performances of them have not been systematically compared. We aim to compare the current models for their predictive potency of mortality at DILI onset. METHODS: DILI patients hospitalized at both Beijing Friendship Hospital and the Fifth Medical Center of PLA General Hospital were categorized into death/liver transplantation (LT) or survival without LT group. Predictive potency of 28-day, 90-day, 6-month and 12-month death/LT outcomes of Hy's Law, nHy's Law, Robles-Diaz Model, drug-induced liver toxicity (DrILTox ALF) Score, Model for End-stage Liver Disease (MELD) Score, and Ghabril Model was compared by Delong method. RESULTS: A total of 6.3% (83/1314) patients died or received LT within 12 months after DILI onset. The area under receiver operating characteristic of Hy's Law, nHy's Law, and Robles-Diaz Model was all lower than 0.750 for the prediction of within 12 months' mortality. DrILTox ALF Score, MELD Score and Ghabril Model showed better predictive potency of 28-day [0.896 (0.878-0.912), 0.934 (0.919-0.947), 0.935 (0.921-0.948), respectively], 90-day [0.883 (0.864-0.899), 0.951 (0.938-0.962), 0.952 (0.939-0.963), respectively], 6-month [0.820 (0.799-0.841), 0.905 (0.888-0.921) and 0.908 (0.891-0.923), respectively] and 12-month [0.801 (0.779-0.823), 0.882 (0.863-0.899) and 0.885 (0.866-0.902), respectively] mortality. CONCLUSION: Despite the difference of clinical characteristics and implicated-drug categories between China and industrialized countries, we demonstrate that MELD Score and Ghabril Model have the best predictive performance in the prediction of mortality within 12 months after DILI onset.

17.
Circulation ; 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36317529

RESUMO

BACKGROUND: The sinoatrial node (SAN) functions as the pacemaker of the heart, initiating rhythmic heartbeats. Despite its importance, the SAN is one of the most poorly understood cardiac entities because of its small size and complex composition and function. The Hippo signaling pathway is a molecular signaling pathway fundamental to heart development and regeneration. Although abnormalities of the Hippo pathway are associated with cardiac arrhythmias in human patients, the role of this pathway in the SAN is unknown. METHODS: We investigated key regulators of the Hippo pathway in SAN pacemaker cells by conditionally inactivating the Hippo signaling kinases Lats1 and Lats2 using the tamoxifen-inducible, cardiac conduction system-specific Cre driver Hcn4CreERT2 with Lats1 and Lats2 conditional knockout alleles. In addition, the Hippo-signaling effectors Yap and Taz were conditionally inactivated in the SAN. To determine the function of Hippo signaling in the SAN and other cardiac conduction system components, we conducted a series of physiological and molecular experiments, including telemetry ECG recording, echocardiography, Masson Trichrome staining, calcium imaging, immunostaining, RNAscope, cleavage under targets and tagmentation sequencing using antibodies against Yap1 or H3K4me3, quantitative real-time polymerase chain reaction, and Western blotting. We also performed comprehensive bioinformatics analyses of various datasets. RESULTS: We found that Lats1/2 inactivation caused severe sinus node dysfunction. Compared with the controls, Lats1/2 conditional knockout mutants exhibited dysregulated calcium handling and increased fibrosis in the SAN, indicating that Lats1/2 function through both cell-autonomous and non-cell-autonomous mechanisms. It is notable that the Lats1/2 conditional knockout phenotype was rescued by genetic deletion of Yap and Taz in the cardiac conduction system, and these rescued mice had normal sinus rhythm and reduced fibrosis of the SAN, indicating that Lats1/2 function through Yap and Taz cleavage under targets and tagmentation sequencing data showed that Yap potentially regulates genes critical for calcium homeostasis such as Ryr2 and genes encoding paracrine factors important in intercellular communication and fibrosis induction such as Tgfb1 and Tgfb3. Consistent with this, Lats1/2 conditional knockout mutants had decreased Ryr2 expression and increased Tgfb1 and Tgfb3 expression compared with control mice. CONCLUSIONS: We reveal, for the first time to our knowledge, that the canonical Hippo-Yap pathway plays a pivotal role in maintaining SAN homeostasis.

18.
Food Chem ; 405(Pt A): 134809, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36368097

RESUMO

Aging is an indispensable process to endow Huangjiu with harmonious and pleasant fragrances. To elucidate the effects of aging time on aroma characteristics of Xiecun Huangjius, 5 samples aged for different years (3 to 9 years) were investigated by sensory evaluation, gas chromatography-mass spectrometry-olfactometry/detection frequency (GC-MS-O/DF), and odor activity values (OAV) analysis. Sensory results showed significant differences existed among different aged Xiecun Huangjius. Totally, 54 compounds in Xiecun Huangjius were identified by GC-MS and further quantified. Of these, 16 were confirmed as key aroma-active compounds with high OAVs and DFs. Partial least squares regression (PLSR) indicated that the old-aged Y9 sample was greatly related to most aroma-active compounds, thus presented the coordinated overall flavor. Together with orthogonal partial least squares-discriminant analysis (OPLS-DA), 4 compounds (diethyl succinate, furfural, nonanal and isoamyl alcohol) were determined as the main markers for classification of the old-aged Y9 and the other-aged samples.

19.
Artigo em Inglês | MEDLINE | ID: mdl-36376217

RESUMO

BACKGROUND/PURPOSE: Clinical data on carbapenem-resistant Enterobacterales (CRE) bacteremia in the pediatric population are limited. This study investigated the clinical characteristics and outcomes of pediatric CRE bacteremia. METHODS: Clinical data on bacteremia caused by carbapenem-susceptible and carbapenem-resistant Enterobacterales, including Escherichia coli, Klebsiella spp., Enterobacter spp., Serratia marcescens, Proteus mirabilis, Citrobacter spp., and Morganella spp., in pediatric patients from a children's hospital in Taiwan were retrospectively retrieved and analyzed. RESULTS: From January 2013 to December 2021, 471 clinical isolates of Enterobacterales bacteremia were identified in 451 episodes from 379 pediatric patients. Among all the isolates, the predominant species were E. coli (199/471, 42.2%), Klebsiella spp. (168/471, 35.6%), and Enterobacter spp. (59/471, 12.5%), with carbapenem-resistance rates of 1.5%, 11.9%, and 25.0%, respectively. Overall, 40 (8.4%) showed a carbapenem resistance phenotype. Patients' all-cause mortality rate at 14 days was significantly higher in CRE bacteremia episodes than non-CRE ones (12.5% vs. 3.6%, p < 0.05). The predicting factor of a CRE bacteremia episode was the causative agent of Enterobacter spp. (adjusted OR of 2.551, CI 1.073-6.066, p < 0.05) and ESBL-producing phenotype (adjusted OR 14.268, CI 5.120-39.762, p < 0.001). CONCLUSION: Bloodstream infections caused by CRE are associated with a higher mortality rate in the pediatric population. Attention must be paid to preventing and managing pediatric patients with CRE infections.

20.
Infect Drug Resist ; 15: 6471-6483, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36353378

RESUMO

Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infection has proven to be difficult to control and typically presents with devastating effects. Methods: This retrospective study was conducted on the renal recipients at our institution between January 2021 to January 2022. Clinical data was collected to identify factors associated with CRKP infection and clinical outcomes. Results: There were 104 cases out of 186 total renal recipients who presented with at least one infection within 3 months after KT, and 14 cases developed unfavorable clinical outcomes. We identified 16 confirmed CRKP infected cases with the incidence of 8.60%. Possible donor derived infection (DDI) (OR = 6.743; 95% CI: 1.477-30.786; P = 0.014) were independent risk factors for the occurrence of CRKP infection of renal recipients in our analysis, CRKP infection (OR = 20.723; 95% CI: 3.448-124.547; P = 0.001) and pneumonia (OR = 28.458; 95% CI: 1.956-413.984 P = 0.014) were independent risk factors for the occurrence of unfavorable clinical outcomes following KT, and the occurrence of unfavorable clinical outcomes following KT were significantly associated with CRKP infection (r = 0.535; P < 0.001) and antibiotic regimen containing ceftazidime/avibactam (CZA) (r = -0.655; P = 0.006). The use of CZA was significantly different in the comparison of antibiotic regimens between the CRKP infected renal recipients with unfavorable outcomes and CRKP infected patients with favorable outcomes. Conclusion: It is possible that DDI can lead to CRKP infection, and CRKP infection and pneumonia were closely correlated with poor prognosis. The use of CZA may play a role in avoiding the unfavorable outcomes of CRKP infected recipients.

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