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1.
Artigo em Inglês | MEDLINE | ID: mdl-31954517

RESUMO

MicroRNA-25-3p (miR-25-3p) has been reported to be closely related with oxidative stress and apoptosis. Here, we aimed to detect the effects of miR-25-3p in the primarily cultured hippocampal neurons. Kainic acid (KA) was used to induce epileptic seizures in the rats. We predicted that oxidative stress responsive 1 (OXSR1) might be a potential target of miR-25-3p with TargetScan prediction and luciferase assays, and the primarily cultured hippocampal neurons were exposed to Mg2+-free solution for 3 h to induce spontaneous recurrent epileptiform discharges (SREDs). Then, the expression of miR-25-3p and OXSR1 in the rats hippocampi and primarily cultured hippocampal neurons were detected. Those SREDs neurons were treated with miR-25-3p mimic, miR-25-3p inhibitor or/and OXSR1 over-expression vector, and SREDs, oxidative stress and apoptosis were observed. We found down-regulation of miRNA-25-3p and up-regulation of OXSR1 in hippocampi of KA-treated rats and Mg2+-free-treated neurons. MiRNA-25-3p mimic could down-regulate OXSR1 expression, inhibit SREDs, reduce oxidative stress and decrease apoptosis. Additionally, over-expression of OXSR1 weakened those effects of miR-25-3p mimic. Those data indicated that miR-25-3p had anti-epileptic, anti-oxidant and anti-apoptosis effects on the primarily cultured neurons through targeting OXSR1, which provided a novel target for the treatment of epilepsy.

2.
Sensors (Basel) ; 20(2)2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31947587

RESUMO

In this paper, a range-based cooperative localization method is proposed for multiple platforms of various structures. The localization system of an independent platform might degrade or fail due to various reasons such as GPS signal-loss, inertial measurement unit (IMU) accumulative errors, or emergency reboot. It is a promising approach to solve this problem by using information from neighboring platforms, thus forming a cooperative localization network that can improve the navigational robustness of each platform. Typical ranging-based ultra-wideband (UWB) cooperative localization systems require at least three auxiliary nodes to estimate the pose of the target node, which is often hard to meet especially in outdoor environment. In this work, we propose a novel IMU/UWB-based cooperative localization solution, which requires a minimum number of auxiliary nodes that is down to 1. An Adaptive Ant Colony Optimization Particle Filter (AACOPF) algorithm is customized to integrate the dead reckoning (DR) system and auxiliary nodes information with no prior information required, resulting in accurate pose estimation, while to our knowledge the azimuth have not been estimated in cooperative localization for the insufficient observation of the system. We have given the condition when azimuth and localization are solvable by analysis and by experiment. The feasibility of the proposed approach is evaluated through two filed experiments: car-to-trolley and car-to-pedestrian cooperative localization. The comparison results also demonstrate that ACOPF-based integration is better than other filter-based methods such as Extended Kalman Filter (EKF) and traditional Particle Filter (PF).

3.
Sci China Life Sci ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31942687

RESUMO

In China, the medical guidelines recommend performing noninvasive prenatal testing (NIPT) with caution for pregnant women aged 35 years or older. However, the Mother and Child Health Care Law suggests that all primiparous women whose age is older than 35 years undergo prenatal diagnosis. These two inconsistent suggestions/recommendations have made obstetricians confused about whether to offer NIPT to these older pregnant women. To face this issue and find out the solution we performed a retrospective study of 189,809 NIPT samples collected from 28 provincial-leveled administrative units in China. Of 1,564 women with high-risk pregnancies who underwent NIPT, 459 (29.3%) did not participate in follow-up. The compound sensitivity and specificity of NIPT for trisomies 21, 18 and 13 detection was 99.1% (95% CI, 98.0%-99.6%) and 99.9% (95% CI, 98.8%-99.9%), respectively. In secundiparous women, NIPT showed high sensitivity and specificity similar to that in primiparous women. The observed risk for trisomies 21 and 18 significantly increased when the maternal age was 39 and older. After the publication of the current NIPT policy, the follow-up rate at our center was 91.9%; however, a large number of women are not in maternal and infant care networks nationwide, and that makes the follow-up rate outside our center relatively low. Our study shows that to balance the prevention of major aneuploidies and the limited resources for prenatal diagnosis, the cut-off age of 35 for invasive prenatal diagnosis might be unnecessary. Although the NIPT guidelines are well written, how to practice it effectively, especially in less industrialized areas, is worth discussing.

4.
Artif Cells Nanomed Biotechnol ; 48(1): 393-407, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31913710

RESUMO

LncRNA PTENP1 is a competitive endogenous RNA (ceRNA) involved in decoying miR-106b in multiple diseases. This study investigates the interaction of PTENP1 and miR-106b in cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) in cervical cancer. The expressions of PTENP1, miR-106b and PTEN were determined in cervical cancer tissues, adjacent normal tissues, cervical cancer cells (HeLa, SiHa, C33A and CasKi) and normal cervical epithelial H8 cells. Up-regulation of PTENP1 and down-regulation of miR-106b were conducted in HeLa and CasKi cells by transfecting cells with corresponding miRNA mimics and inhibitors. Bioinformatics analysis, luciferase reporter assay and RNA-pull down assay were performed to verify the association of miR-106b, PTEN, and PTENP1. Cell growth and cell apoptosis were determined by CCK-8 and flow cytometry analysis. It was found that the expressions of PTENP1 and PTEN were up-regulated and that of miR-106b were down-regulated in cervical cancer tissues and cells. PTENP1 localized in cytoplasm and competitively bound to miR-106b. Up-regulation of PTENP1 and down-regulation of miR-106b contributed to increased expressions of PTEN and E-cadherin. Decreased expression of miR-106b, ZEB1, Snail and Vimentin, resulted in inhibiting cell proliferation and promoting cell apoptosis. Over-expression of PTENP1 and miR-106b accelerated cell proliferation and slowed down cell apoptosis. miR-106b inhibited the expression of PTEN. Our results suggest that LncRNA PTENP1 inhibits cervical cancer progression by competitively binding to miR-106b, leading to promote PTEN expression, inhibit cell proliferation and EMT and induce cell apoptosis in cervical cancer cells.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31916744

RESUMO

Injection of phase transition from layered to rock salt phase into bulk lattice and side reactions on the interfacial usually cause structure degradation, quick capacity/voltage decay and even thermal instability. Here, self-formed interfacial protective layer coupled with lattice tuning was constructed for Ni-rich cathode by simultaneous incorporation of Zr and Al in one calcination step. The migration energy between Zr and Al from surface into bulk lattice induces dual modifications from surface into bulk lattice, which effectively decrease the formation of cation mixing, the degree of anisotropic lattice change and the generation of microcracks. With the stabilization role provided by the doped Zr-Al ions and protective function endowed by the surface layer, the modified cathode material exhibits significantly enhanced capacity and voltage retention. Specifically, the capacity retention for modified cathode material reaches 99 % after 100 cycles at 1 C at 25 ℃ in the voltage of 3.0~4.3 V, which is outperformed that of 70 % for pristine cathode. The declination of the average voltage for modified cathode is only 0.025 and 0.097 V after 100 cycles at 1 C in the voltage of 3.0~4.3 V and 2.8~4.5 V, which is much lower than that of 0.230 and 0.405 V for pristine cathode. The synchronous accomplishment of modification from surface into bulk lattice for Ni-rich materials with multiple elements in one calcination step would provide some referenced value for the preparation of other cathode materials.

6.
J Agric Food Chem ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31891505

RESUMO

Convenient, portable, and low-cost multiplex nucleic acid testing (NAT) systems are the trends in the fields of food safety, environmental microorganisms, molecular diagnosis, etc. In this study, we developed a novel system for visual monitoring of multiple nucleic acids combining a mini-disk capillary array (diameter = 17 mm, embedded with 6-10 capillaries), visual loop-mediated isothermal amplification (LAMP), and quick DNA extraction called mDC-LAMP. The performance and applicability of mDC-LAMP in testing multiple nucleic acids were evaluated and verified employing genetically modified contents analysis as an example. All of the results confirmed that mDC-LAMP has the advantages of high specificity without any cross contamination, high sensitivity with a limit of detection of 25 copies/reaction, high throughput with flexible channel sensors, easy fabrication, and low costs. We believe that mDC-LAMP is a competitive choice for on-spot monitoring of multiple nucleic acids in terms of the easy fabrication/operation, low costs, and suitable performance presented in the nucleic acids test.

7.
Annu Rev Biophys ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31928428

RESUMO

Mitochondria are essential organelles in eukaryotes. Most mitochondrial proteins are encoded by the nuclear genome and translated in the cytosol. Nuclear-encoded mitochondrial proteins need to be imported, processed, folded, and assembled into their functional states. To maintain protein homeostasis (proteostasis), mitochondria are equipped with a distinct set of quality control machineries. Deficiencies in such systems lead to mitochondrial dysfunction, which is a hallmark of aging and many human diseases, such as neurodegenerative diseases, cardiovascular diseases, and cancer. In this review, we discuss the unique challenges and solutions of proteostasis in mitochondria. The import machinery coordinates with mitochondrial proteases and chaperones to maintain the mitochondrial proteome. Moreover, mitochondrial proteostasis depends on cytosolic protein quality control mechanisms during crises. In turn, mitochondria facilitate cytosolic proteostasis. Increasing evidence suggests that enhancing mitochondrial proteostasis may hold therapeutic potential to protect against protein aggregation-associated cellular defects. Expected final online publication date for the Annual Review of Biophysics, Volume 49 is May 6, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

8.
J Environ Manage ; 258: 110014, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31929056

RESUMO

The carbon to nitrogen ratio (C/N) is well known for its importance in the composting process, however the fiber degradation and humification associated with enzymatic activity and microbial variation derived from different C/N ratios are poorly studied. Here, we designed two treatments of chicken manure with 15% (initial C/N ratio 9.61) and 50% (initial C/N ratio 17.3) rice husk to adjust the moisture of mixtures for turning feasibly by towable fertilizer turner in industrial level. Compared to the C/N ratio 9.61, the suitable C/N ratio of 17.3 significantly enhanced the composting efficiency and the final germination index (23.7%). Moreover, the suitable C/N ratio increased the relative abundance of Bacilli, which played an important role during the mesophilic and thermophilic phases. Bacilli abundance was related to cellulose and ß-glycosidase activities, thus improved fiber degradation and humification. This study not only seeks a swift method in industrial level to process chicken manure but also provides insight into the enzymatic activity of microbial community related to high-efficient composting.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31898447

RESUMO

Taking the advantages of excellent optical properties, biocompatibility, and photostability of carbon dots, herein, we developed polarity-sensitive polymer carbon dots (PCDs) for visualizing of cellular polarity to real-time monitoring autophagy changes without perturbing the cellular status. The PCDs can be prepared by simply mixing dopamine (DA), H2O2, and o-phenylenediamine (o-PDA) in a common beaker without the need for any special equipment or external energy supply, and the preparation could be completed within 3 min at room temperature. Interestingly, the polarity-sensitive PCDs could emit various types of fluorescence and are insensitive to the excitation light when dispersed in different water/dioxane systems with different polarities. Based on the polarity-sensitive emission of the PCDs, the change of polarity during autophagy has been successfully monitored in living cells. Moreover, the change of polarity detected by PCDs is autophagy-specific (does not occur during apoptosis), occurs under different autophagy-inducing situations (starvation, rapamycin, and trehalose), and requires a normal autophagic flux, showing that PCDs rapidly prepared by polymerization cross-linking at room temperature can be functionally applied in the case of autophagy-related physiological or pathological processes.

10.
Nat Commun ; 11(1): 277, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31937754

RESUMO

Migration of meiosis-I (MI) spindle from the cell center to a sub-cortical location is a critical step for mouse oocytes to undergo asymmetric meiotic cell division. In this study, we investigate the mechanism by which formin-2 (FMN2) orchestrates the initial movement of MI spindle. By defining protein domains responsible for targeting FMN2, we show that spindle-periphery localized FMN2 is required for spindle migration. The spindle-peripheral FMN2 nucleates short actin bundles from vesicles derived likely from the endoplasmic reticulum (ER) and concentrated in a layer outside the spindle. This layer is in turn surrounded by mitochondria. A model based on polymerizing actin filaments pushing against mitochondria, thus generating a counter force on the spindle, demonstrated an inherent ability of this system to break symmetry and evolve directional spindle motion. The model is further supported through experiments involving spatially biasing actin nucleation via optogenetics and disruption of mitochondrial distribution and dynamics.

11.
Psychother Psychosom ; 89(1): 38-47, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31846980

RESUMO

BACKGROUND: Not all adults with chronic insomnia respond to the recommended therapeutic options of cognitive behavioral therapy and approved hypnotic drugs. Transcranial alternating current stimulation (tACS) may offer a novel potential treatment modality for insomnia. OBJECTIVES: This study aimed to examine the efficacy and safety of tACS for treating adult patients with chronic insomnia. METHODS: Sixty-two participants with chronic primary insomnia received 20 daily 40-min, 77.5-Hz, 15-mA sessions of active or sham tACS targeting the forehead and both mastoid areas in the laboratory on weekdays for 4 consecutive weeks, followed by a 4-week follow-up period. The primary outcome was response rate measured by the Pittsburgh Sleep Quality Index (PSQI) at week 8. Secondary outcomes were remission rate, insomnia severity, sleep onset latency (SOL), total sleep time (TST), sleep efficiency, sleep quality, daily disturbances, and adverse events at the end of the 4-week intervention and at the 4-week follow-up. RESULTS: Of 62 randomized patients, 60 completed the trial. During the 4-week intervention, 1 subject per group withdrew due to loss of interest and time restriction, respectively. Based on PSQI, at 4-week follow-up, the active group had a higher response rate compared to the sham group (53.4% [16/30] vs. 16.7% [5/30], p = 0.009), but remission rates were not different between groups. At the end of the 4-week intervention, the active group had higher response and remission rates than the sham group (p < 0.001 and p = 0.026, respectively). During the trial, compared with the sham group, the active group showed a statistically significant decrease in PSQI total score, a shortened SOL, an increased TST, improved sleep efficiency, and improved sleep quality (p < 0.05 or p < 0.001). Post hoc analysis revealed that, in comparison with the sham group, the active group had improved symptoms, except for daily disturbances, at the end of the 4-week intervention, and significant improvements in all symptoms at the 4-week follow-up. No adverse events or serious adverse responses occurred during the study. CONCLUSION: The findings show that the tACS applied in the present study has potential as an effective and safe intervention for chronic insomnia within 8 weeks.

12.
J Sci Food Agric ; 100(2): 614-622, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31597198

RESUMO

BACKGROUND: Lonicera japonica Thunb is a common herb in East Asia. The flower buds are usually regarded as the traditional medicinal part, while leaves and stems are considered less valuable and receive little attention. This study compared the chemical constituents and anti-inflammatory effects of the different tissues in L. japonica Thunb for the first time. RESULTS: Thirty compounds were identified by ultra-performance liquid chromatography-photodiode detector-quadrupole / time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS/MS) analysis. Hydroxycinnamic acids, flavonoids, and iridoids were identified as the major components. The flower buds (FLJ), leaves (LLJ), and stems (SLJ) of L. japonica Thunb showed strong similarities in chemical components. The LLJ contained higher levels of hydroxycinnamic acids and flavonoids than the FLJ and SLJ. Furthermore, FLJ, LLJ, and SLJ exhibited potent anti-inflammatory activity in croton oil-induced ear edema and carrageenan-induced paw edema assays in mice. Moreover, FLJ, LLJ, and SLJ showed a cytoprotective effect on lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophages. Lipopolysaccharide-induced increases in nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and interleukin-6 (IL-6) were suppressed by treatments of FLJ, LLJ, and SLJ, respectively. The LLJ possessed a stronger anti-inflammatory effect than the FLJ. CONCLUSION: Leaves and stems of L. japonica Thunb have chemical components and anti-inflammatory properties similar to flower buds, and may become alternative or supplementary sources of flower buds. © 2019 Society of Chemical Industry.


Assuntos
Anti-Inflamatórios/química , Edema/tratamento farmacológico , Lonicera/química , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Animais , Anti-Inflamatórios/administração & dosagem , Carragenina/efeitos adversos , Cromatografia Líquida de Alta Pressão , Edema/induzido quimicamente , Edema/genética , Edema/imunologia , Flavonoides/administração & dosagem , Flavonoides/química , Flores/química , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Interleucina-6/genética , Interleucina-6/imunologia , Camundongos , Folhas de Planta/química , Caules de Planta/química , Espectrometria de Massas em Tandem , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia
13.
Sheng Wu Gong Cheng Xue Bao ; 35(11): 2133-2150, 2019 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-31814360

RESUMO

In recent years, antibiotics have been widely used in animal husbandry, aquaculture and the medication in China. Many antibiotics are discharged into the environment, resulting in dramatic increase of antibiotic residues in domestic water and soil. Residues of different antibiotics in the environment change the microbial structure, which is extremely harmful to the ecological environment and humans. Therefore, remediation of antibiotic contamination is significantly important. Studies have shown that some microorganisms can degrade and utilize antibiotics, and thus have good application prospects on bioremediation of antibiotic contamination. However, little is known about the microbial degradation mechanism of antibiotics. This article summarizes the removal of antibiotics by antibiotic-degrading strains and bacterial flora in recent ten years, and the methods of using microbial flora to treat antibiotic residues. The future prospect of using microbial remediation to reduce antibiotic residues in the environment has also been discussed.

14.
Shanghai Kou Qiang Yi Xue ; 28(4): 408-411, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31792483

RESUMO

PURPOSE: To detect the gene expression of miRNAs in patients with periodontitis and to explore their biological functions and involved signaling pathways. METHODS: Bioinformatics analysis of gene chip data from 158 periodontitis patients and 40 healthy controls of the microarray database GSE54710 were performed. The expression changes of miRNAs were analyzed. The involved biological function and signal path was predicted. SPSS 19.0 software package was used for statistical analysis. RESULTS: Five miRNAs (hsa-miR-451, hsa-miR-223, hsa-miR-486-5p, hsa-miR-3917, hsa-miR-671-5p) were significantly up-regulated, and 4 miRNAs (hsa-miR-203, hsa-miR-210, hsa-miR-1246, hsa-miR-1260) were significantly down-regulated. Among them, there were 584 target genes of hsa-miR-1260 and 139 target genes of hsa-miR-451. KEGG pathway enrichment analysis showed that hsa-miR-1260 target gene was significantly enriched into 12 signaling pathways such as TGF-beta, and hsa-miR-451 target gene was significantly enriched into 17 signaling pathways. CONCLUSIONS: miRNAs expression profiles were obtained in periodontitis tissues, periodontitis-induced hsa-miR-1260 and hsa-miR-451 may play a key role in the pathophysiology of periodontitis.

15.
Cancer Commun (Lond) ; 39(1): 81, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31796105

RESUMO

BACKGROUND: High-grade glioma (HGG) is a fatal human cancer. Bortezomib, a proteasome inhibitor, has been approved for the treatment of multiple myeloma but its use in glioma awaits further investigation. This study aimed to explore the chemotherapeutic effect and the underlying mechanism of bortezomib on gliomas. METHODS: U251 and U87 cell viability and proliferation were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, tumor cell spheroid growth, and colony formation assay. Cell apoptosis and cell cycle were detected by flow cytometry. Temozolomide (TMZ)-insensitive cell lines were induced by long-term TMZ treatment, and cells with stem cell characteristics were enriched with stem cell culture medium. The mRNA levels of interested genes were measured via reverse transcription-quantitative polymerase chain reaction, and protein levels were determined via Western blotting/immunofluorescent staining in cell lines and immunohistochemical staining in paraffin-embedded sections. Via inoculating U87 cells subcutaneously, glioma xenograft models in nude mice were established for drug experiments. Patient survival data were analyzed using the Kaplan-Meier method. RESULTS: Bortezomib inhibited the viability and proliferation of U251 and U87 cells in a dose- and time-dependent manner by inducing apoptosis and cell cycle arrest. Bortezomib also significantly inhibited the spheroid growth, colony formation, and stem-like cell proliferation of U251 and U87 cells. When administrated in combination, bortezomib showed synergistic effect with TMZ in vitro and sensitized glioma to TMZ treatment both in vitro and in vivo. Bortezomib reduced both the mRNA and protein levels of Forkhead Box M1 (FOXM1) and its target gene Survivin. The FOXM1-Survivin axis was markedly up-regulated in established TMZ-insensitive glioma cell lines and HGG patients. Expression levels of FOXM1 and Survivin were positively correlated with each other and both related to poor prognosis in glioma patients. CONCLUSIONS: Bortezomib was found to inhibit glioma growth and improved TMZ chemotherapy efficacy, probably via down-regulating the FOXM1-Survivin axis. Bortezomib might be a promising agent for treating malignant glioma, alone or in combination with TMZ.

16.
Phytother Res ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31833107

RESUMO

Berberine (BBR), a small alkaloid, is used as a hypoglycemic agent in China. Stachyose (Sta), a Rehmannia glutinosa oligosaccharide, acts as a prebiotic. This study aimed to evaluate whether BBR combined with Sta produced better glycometabolism than BBR alone, and explored the effects on gut microbiota and metabolomics. Type-2 diabetic db/db mice were administered BBR (100 mg/kg), Sta (200 mg/kg), or both by gavage once daily. Glucose metabolism, the balance of α- and ß-cells, and mucin-2 expression were ameliorated by combined treatment of BBR and Sta, with stronger effects than upon treatment with BBR alone. The microbial diversity and richness were altered after combined treatment and after treatment with BBR alone. The abundance of Akkermansia muciniphila was increased by combined treatment compared to treatment with BBR alone, while the levels of the metabolite all-trans-heptaprenyl diphosphate were decreased and the levels of fumaric acid were increased, which both showed a strong correlation with A. muciniphila. In summary, BBR combined with Sta produced better glycometabolism than BBR alone through modulating gut microbiota and fecal metabolomics, and may aid in the development of a novel pharmaceutical strategy for treating Type 2 diabetes mellitus.

17.
Org Biomol Chem ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31833525

RESUMO

The general protocol for the synthesis of isoxazolidine-fused isoquinolin-1(2H)-ones was established with the help of bench stable hypervalent iodine reagent PIDA. Polycyclic six-, seven- and eight-membered N-heterocycles can be rapidly synthesized from available amides under metal-free conditions within 1 min at room temperature through C-H/N-H functionalization. Moreover, the protocol has the merits of broad substrate scope, atom economy and operational simplicity.

18.
J Cancer ; 10(27): 6822-6828, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31839816

RESUMO

The coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) is overexpressed in several types of cancer. This study aimed to investigate the role of CHCHD2 in hepatocellular carcinoma (HCC). The expression of CHCHD2 in HCC and non-tumorous tissues was detected by immunohistochemistry and Western blot analysis, and the correlation between CHCHD2 expression and clinicopathological features of HCC was analyzed. Furthermore, the proliferation, apoptosis and migration of HepG2 cells with CHCHD2 knockdown were examined. We found that CHCHD2 was upregulated in HCC tissues, and high CHCHD2 expression was associated with poor differentiation, lymph node metastasis, local tissue invasion, high TNM grade of HCC and poor patient survival. Depletion of CHCHD2 led to significantly reduced cell proliferation, increased apoptosis and diminished migratory capacity in HepG2 cells. In addition, HCC tissues had high expression of CD105, a microvessel marker, and HepG2 cells depleted of CHCHD2 had low CD105 expression. In conclusion, CHCHD2 may play an oncogenic role in HCC via promoting tumor cell growth and migration while preventing apoptosis. CHCHD2 is a potential biomarker for poor outcome of HCC patients.

19.
Neurochem Res ; 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31823113

RESUMO

Neuroinflammation is critical in the pathogenesis of neurological diseases. Microglial pro-inflammatory (M1) and anti-inflammatory (M2) status determines the outcome of neuroinflammation. Dexmedetomidine exerts anti-inflammatory effects in many neurological conditions. Whether dexmedetomidine functions via modulation of microglia M1/M2 polarization remains to be fully elucidated. In the present study, we investigated the anti-inflammatory effects of dexmedetomidine on the neuroinflammatory cell model and explored the potential mechanism. BV2 cells were stimulated with LPS to establish a neuroinflammatory model. The cell viability was determined with MTT assay. NO levels were assessed using a NO detection kit. The protein levels of IL-10, TNF-α, iNOS, CD206, ERK1/2, and pERK1/2 were quantified using Western blotting. LPS significantly increased pro-inflammatory factors TNF-α and NO, and M1 phenotypic marker iNOS, and decreased anti-inflammatory factor IL-10 and M2 phenotypic marker CD206 in BV2 cells. Furthermore, exposure of BV2 cells to LPS significantly raised pERK1/2 expression. Pretreatment with dexmedetomidine attenuated LPS-elicited changes in p-ERK, iNOS, TNF-α, NO, CD206 and IL-10 levels in BV2 cells. However, co-treatment with dexmedetomidine and LM22B-10, an agonist of ERK, reversed dexmedetomidine-elicited changes in p-ERK, iNOS, TNF-α, NO, CD206 and IL-10 levels in LPS-exposed BV2 cells. We, for the first time, showed that dexmedetomidine increases microglial M2 polarization by inhibiting phosphorylation of ERK1/2, by which it exerts anti-inflammatory effects in BV2 cells.

20.
J Agric Food Chem ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31830780

RESUMO

Cholesterol was usually used to stabilize liposome, while there have been controversy on the relationship between dietary cholesterol and health. The present study aimed to prepare a novel multifunctional nanoliposomes stabilized by sea cucumber-derived saponins using ultrasound-assisted film dispersion method. A novel uniform liposome with mass ratio of egg yolk lecithin/sea cucumber saponins at 75:25 was successfully prepared to encapsulate saponin, and the particle size was 164.8 ± 1.70 nm with PDI value of 0.214 ± 0.022 and zeta potential of -15.97 ± 1.23 mV. The digestion and absorption results in vivo showed dietary saponins in liposome form could delay the peak time of saponins and prolong their residence time in serum. Moreover, saponins were more easily converted into their corresponding metabolites after administration with saponins in liposome form. The novel liposome as an efficient carrier with multi-functions had great potential in the development of functional food and biomedical applications.

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