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1.
Neural Regen Res ; 18(4): 881-888, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36204858

RESUMO

Ferroptosis is one of the critical pathological events in spinal cord injury. Erythropoietin has been reported to improve the recovery of spinal cord injury. However, whether ferroptosis is involved in the neuroprotective effects of erythropoietin on spinal cord injury has not been examined. In this study, we established rat models of spinal cord injury by modified Allen's method and intraperitoneally administered 1000 and 5000 IU/kg erythropoietin once a week for 2 successive weeks. Both low and high doses of erythropoietin promoted recovery of hindlimb function, and the high dose of erythropoietin led to better outcome. High dose of erythropoietin exhibited a stronger suppressive effect on ferroptosis relative to the low dose of erythropoietin. The effects of erythropoietin on inhibiting ferroptosis-related protein expression and restoring mitochondrial morphology were similar to those of Fer-1 (a ferroptosis suppressor), and the effects of erythropoietin were largely diminished by RSL3 (ferroptosis activator). In vitro experiments showed that erythropoietin inhibited RSL3-induced ferroptosis in PC12 cells and increased the expression of xCT and Gpx4. This suggests that xCT and Gpx4 are involved in the neuroprotective effects of erythropoietin on spinal cord injury. Our findings reveal the underlying anti-ferroptosis role of erythropoietin and provide a potential therapeutic strategy for treating spinal cord injury.

2.
EClinicalMedicine ; 55: 101723, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36386034

RESUMO

Background: The pathophysiology and subsequent myocardial dysfunction of heart failure with preserved ejection fraction (HFpEF) with comorbid obesity has not been extensively described. This study aimed to investigate the clinical features and cardiovascular magnetic resonance (CMR) derived myocardial strain and tissue characteristics in patients with HFpEF and comorbid obesity phenotype. Methods: In this prospective cohort study, we included consecutive patients admitted to Fuwai hospital in China who underwent CMR. Patients with HFpEF or obesity were diagnosed with demographic data, clinical presentation, laboratory test, and echocardiography or CMR imaging. The key exclusion criteria were cardiomyopathy, primary valvular heart disease, and significant coronary artery disease. Participant data were obtained from the electronic medical records database or inquiry. Comparisons of clinical features and CMR derived structural and functional parameters amongst different groups were made using one-way analysis of variance, or χ2 tests, and post hoc Bonferroni analysis where appropriate. Findings: Between January 1, 2019 and July 31, 2021, 280 participants (108 patients with HFpEF and obesity, 50 patients with HFpEF and normal weight, 72 patients with obesity, and 50 healthy controls) were enrolled. Compared with patients with HFpEF and normal weight, patients with HFpEF and obesity were younger males, and had higher plasma volume, uric acid and hemoglobin levels, yet less often atrial fibrillation, and lower NT-proBNP levels, and had higher left ventricular mass index, end-diastole/systole volume index, lower left atrial volume index, and worse myocardial strains (all p ≤ 0.05), but no remarkable difference in late gadolinium enhancement (LGE) presence and extracellular volume fraction (ECV). After adjusting for age, atrial fibrillation, and coronary artery disease, only global longitudinal strain (GLS, p = 0.031) and early-diastolic global longitudinal strain rate (eGLSR, p = 0.043) were considerably worse in patients with HFpEF and obesity versus patients with HFpEF and normal weight. Furthermore, early-diastolic strain rates showed no linear association with ECV in patients with HFpEF and obesity. Moreover, GLS demonstrated the highest diagnostic ability when compared with traditional CMR structural parameters and ECV to diagnose patients with HFpEF and obesity in the setting of obesity. Interpretation: Higher systemic inflammation, and worse GLS and eGLSR may be the distinct features of obesity-related HFpEF phenotype; strains and ECV may represent different mechanisms of HFpEF with obesity, deserving further study. Funding: The Construction Research Project of Key Laboratory (Cultivation) of Chinese Academy of Medical Sciences (2019PT310025); National Natural Science Foundation of China (81971588); Capital's Funds for Health Improvement and Research (CFH 2020-2-4034); Youth Key Program of High-level Hospital Clinical Research (2022-GSP-QZ-5).

3.
Behav Brain Res ; 436: 114075, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36029892

RESUMO

Regret is a common negative emotion in daily life, and long-term immersion in regret affects mental health. Therefore, to regulate and reduce regret is of wide concern. The current fMRI study aimed to investigate whether outcome anticipation before decision-making could reduce regret and its neural correlates. In the task, participants were asked to anticipate the possible poor outcomes of subsequent decisions, such as missing rewards and meeting punishment, and then made sequential risk-taking decisions. Behavioral results showed that outcome anticipation before decision-making could decrease the intensity of regret, that is, participants felt less regret when they anticipated the outcome before decision-making (anticipation condition, Ant), compared to making sequential risk-taking decisions without any anticipation of the outcome in advance (non-anticipation condition, NAnt). Consistently, at the neural level, stronger activities of ventral striatum (VS) and dorsal medial prefrontal cortex (dmPFC), and greater VS-dmPFC functional connectivity were observed in Ant relative to NAnt. Moreover, the activity of dmPFC was negatively correlated with the intensity of regret in Ant. The current study highlighted that outcome anticipation before decision-making could regulate regret effectively, and dmPFC played a vital role in this process.


Assuntos
Emoções , Estriado Ventral , Tomada de Decisões/fisiologia , Emoções/fisiologia , Humanos , Imageamento por Ressonância Magnética , Córtex Pré-Frontal/diagnóstico por imagem , Recompensa , Estriado Ventral/diagnóstico por imagem
4.
J Environ Manage ; 325(Pt A): 116491, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36265232

RESUMO

Nonpoint source (NPS) pollution shows spatial scaling effects because it is affected by topography, river networks, and many other factors. Currently, the lack of an integrated methodology for quantifying the scaling effect has become a crucial barrier in evaluating NPS pollution. In this study, a new method was proposed for scaling NPS pollution by integrating hydrological model and hydrological alteration indicators. Nested catchments were delineated by eight-direction algorithm, and a semidistributed hydrological model was used to simulate the interannual process within the drainage area and to obtain data series of runoff, sediment, and total phosphorus (TP) at different spatial scales. In addition, the average, the extrema, the change rate and feature variables of each type of indicators were proposed to quantitatively describe the pattern of NPS pollution at different spatial scales. The results show the coefficients of variation (CVs) of most runoff and TP indicators are 0.6-0.8, while those of sediment vary greatly from 0.4 to 1.6 with the threshold of those indicators being 0.33. With the increase in drainage area, the NPS load-related indicators show an increasing trend, while load intensity indicators show a decreasing trend and their changing patterns are affected by the heterogeneity of topographic or hydrological information included. Based on logarithmic variance of the change rate, 825 km2 was identified as the turning point for scaling transformation where the slope changes dramatically. The proposed methodology comprehensively describes features of the NPS scaling effect that could be utilized for targeted monitoring and control of NPS pollution in other watersheds.


Assuntos
Poluição Difusa , Poluentes Químicos da Água , Poluição Difusa/análise , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Nitrogênio/análise , Rios , Fósforo/análise , China
5.
J Environ Manage ; 325(Pt B): 116533, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36308957

RESUMO

Urban ecological environment is the basis of citizens' survival and development. A rapid and objective urban ecological environment assessment (UEEA) plays an important role in the urban sustainable development and environment protection. This study established an improved urban ecological comfort index (UECIIMP), which is based on our previous UECI and fully composed of four remote sensing indicators: normalized difference vegetation index (NDVI), normalized difference built-up index (NDBI), land surface temperature (LST), and aerosol optical depth (AOD), representing the greenness, dryness, heat, and atmospheric turbidity, respectively. Combining the entropy method and random forest (RF) algorithm, the weights of four indicators were calculated. To improve the accuracy of UECIIMP, the gap-filled quarterly mean results of each indicator with 30m resolution were obtained using the harmonic analysis of time series (HANTS) method and spatial-temporal information fusion based on non-local means filter (STNLFFM). UECIIMP was applied to the Hefei-Nanjing-Hangzhou Region to explore its spatiotemporal changes and response characteristics. Results show that the weights of UECIIMP fluctuate slightly (within 10%) before and after sensitivity analysis, with good stability and reliability. UECIIMP in Hangzhou > Hefei ≈ Nanjing, spring ≈ autumn > summer â‰« winter. From 2009 to 2019, UECIIMP has improved in all 33 districts of the Hefei-Nanjing-Hangzhou Region. The significant improvement of UECIIMP in 2014-2019 is 4.3 times than that in 2009-2014. The correlation between UECIIMP and economic index indicates that economic development has a positive impact on the urban ecological environment. The significant degradation of UECIIMP in the urban expansion area demonstrates a negative impact on the local environment from urban expansion.


Assuntos
Monitoramento Ambiental , Tecnologia de Sensoriamento Remoto , Monitoramento Ambiental/métodos , Entropia , Inosina Monofosfato , Reprodutibilidade dos Testes , China , Aprendizado de Máquina , Cidades
6.
J Hazard Mater ; 442: 130125, 2023 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-36303337

RESUMO

Novel per- and polyfluoroalkyl substances (PFASs) raise global concerns due to their toxic effects on environment and human health. However, researches on analytical methods of novel PFASs are lacking. Here, a kind of selective cationic covalent organic framework (iCOF) was designed and loaded on the surface of cotton as an adsorbent. Then, a simple solid-phase extraction (SPE) method based on the cotton@iCOF was developed for high throughput rapid extraction of six novel PFASs in water samples, coupled with ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) determination. Several important SPE parameters, such as the amount of iCOF, sample pH, desorption conditions and salinity were systematically investigated. Under optimal conditions, the limits of detection and quantification of this SPE-UHPLC-MS/MS method were as low as 0.08-2.14 ng/L and 0.28-7.15 ng/L, respectively. The recoveries were 77.9-117.6 % for the tap water and surface water, and F-53 B in surface water were detected. Notably, this SPE process was rapid (1 h for 500 mL water sample) compared with commercial SPE (normal 2-3 h), owing to little resistance of cotton@iCOF and omission of nitrogen blowing process, and high throughput with 12 samples concurrently extracted. Additionally, various characterization means and density functional theory (DFT) calculations showed that ion-exchange effect, hydrophobic interaction, hydrogen bonding and ordered channel structure synergistically contributed to the PFASs adsorption on cotton@iCOF. The cotton@iCOF-based SPE method with simplicity, rapidity, selectivity and efficiency provided new research ideas for the analysis and control of ionic emerging pollutants in water.


Assuntos
Fluorcarbonetos , Estruturas Metalorgânicas , Humanos , Espectrometria de Massas em Tandem/métodos , Fluorcarbonetos/análise , Extração em Fase Sólida/métodos , Cromatografia Líquida de Alta Pressão/métodos , Água/química
7.
J Orthop Translat ; 38: 23-31, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36313979

RESUMO

Background: Geniposidic acid (GPA), one of the active components of Eucommia ulmoides, promote bone formation and treat osteoporosis by activating farnesoid X receptor (FXR). However, GPA has low oral availability and lack of bone targeting in the treatment of bone related diseases. With the development of modern technology, small molecules, amino acids, or aptamers are used for biological modification of drugs and target cells in bone tissue, which has become the trend of bone targeted research. Methods: In this study, SDSSD (an osteoblast-targeting peptide) were modified in GPA using Fmoc solid-phase synthesis technique to form a new SDSSD-GPA conjugate (SGPA). The bone targeting of SGPA was evaluated using in vivo imaging and cell co-culture. In vitro, the effect of SGPA on cytotoxicity, osteoblastic activity, and mineralization ability were studied in mouse primary osteoblasts (OBs). In vivo, the therapeutic effect of SGPA on osteoporosis using an ovariectomized (OVX) mouse model. The bone mass, histomorphometry, serum biochemical parameters, and the molecular mechanism were evaluated. Results: SGPA was enriched in OBs and tends to accumulate in bone tissue. In vitro, SGPA significantly enhanced the osteogenic activity and mineralization of OBs compared with GPA. In vivo, SGPA enhanced serum BALP and P1NP levels, increased the trabecular bone mass of the mice, and SGPA administration have a higher bone mineralization deposition rate than the GPA-treated mice. Moreover, SGPA significantly activated FXR and Runt-related transcription factor 2 (RUNX2). Conclusions: Collectively, SGPA is enriched into OBs, and promotes bone formation by activating FXR-RUNX2 signalling, effectively treating osteoporosis at relatively low doses. The translational potential of this article: This study demonstrates a more efficient and safe application of GPA in treating osteoporosis, provide a new concept for the bone targeted application of natural compounds.

8.
Methods Mol Biol ; 2568: 1-12, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36227558

RESUMO

Recent technological developments such as cryogenic electron microscopy (Cryo-EM) and X-ray free electron lasers (XFEL) have significantly expanded the available toolkit to visualize large, complex noncoding RNAs and their complexes. Consequently, the quality of the RNA sample, as measured by its chemical monodispersity and conformational homogeneity, has become the bottleneck that frequently precludes effective structural analyses. Here we describe a general RNA sample preparation protocol that combines cotranscriptional RNA folding and RNA-RNA complex assembly, followed by native purification of stoichiometric complexes. We illustrate and discuss the utility of this versatile method in overcoming RNA misfolding and enabling the structural and mechanistic elucidations of the T-box riboswitch-tRNA complexes.


Assuntos
Riboswitch , Conformação de Ácido Nucleico , RNA/química , RNA/genética , Dobramento de RNA , RNA de Transferência/genética
9.
Virol J ; 19(1): 184, 2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36371169

RESUMO

Rabies is a lethal zoonotic disease that is mainly caused by the rabies virus (RABV). Although effective vaccines have long existed, current vaccines take both time and cost to produce. Messenger RNA (mRNA) technology is an emergent vaccine platform that supports rapid vaccine development on a large scale. Here, an optimized mRNA vaccine construct (LVRNA001) expressing rabies virus glycoprotein (RABV-G) was developed in vitro and then evaluated in vivo for its immunogenicity and protective capacity in mice and dogs. LVRNA001 induced neutralizing antibody production and a strong Th1 cellular immune response in mice. In both mice and dogs, LVRNA001 provided protection against challenge with 50-fold lethal dose 50 (LD50) of RABV. With regards to protective efficiency, an extended dosing interval (14 days) induced greater antibody production than 3- or 7-day intervals in mice. Finally, post-exposure immunization against RABV was performed to evaluate the survival rates of dogs receiving two 25 µg doses of LVRNA001 vs. five doses of inactivated vaccine over the course of three months. Survival rate in the LVRNA001 group was 100%, whereas survival rate in the inactivated vaccine control group was only 33.33%. In conclusion, these results demonstrated that LVRNA001 induced strong protective immune responses in mice and dogs, which provides a new and promising prophylactic strategy for rabies.


Assuntos
Vacinas Antirrábicas , Vírus da Raiva , Raiva , Cães , Camundongos , Animais , Vacinas Antirrábicas/genética , RNA Mensageiro , Anticorpos Antivirais , Vírus da Raiva/genética , Vacinas de Produtos Inativados , Formação de Anticorpos
10.
Heliyon ; 8(11): e11279, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36387579

RESUMO

Objective: Spinal cord injury (SCI) often leads to severe physiological and pathological changes in patients. Erxian Decoction (EXD) is effective in the postoperative treatment of spinal cord injury, but its specific mechanism of action is poorly defined. Methods: Network pharmacology and molecular docking were used to predict the potential mechanisms of EXD in SCI. In vivo studies were used to validate the above predictions. For in vivo study, the rats were pretreated with or without EXD (5.76 g/kg, by intragastric gavage). Multiple molecular biological test methods to identify molecular mechanisms. One-way analysis of variance (ANOVA) was used with Bonferroni's post-hoc test to identify the differences between groups. Results: In vivo studies have shown that EXD improved motor function at 7dpi in SCI rats (P < 0.0001), significantly reduced spinal cord edema (P = 0.0139), upregulated 5-HT, GFAP, and TMEM119 expression. Through network pharmacology analysis, we found that Akt1 in EXD plays a role in treating SCI. The underlying mechanism may be the inhibition of apoptosis after activation of Akt1 phosphorylation. Molecular docking revealed that the key compounds could spontaneously bind to the Akt1 protein. Pharmacological inhibition of Akt1 activation by MK-2206, attenuated the anti-apoptotic effect of EXD on SCI in rats (P < 0.0001). Conclusions: EXD inhibits apoptosis by activating Akt1, reduce spinal cord edema and restore behavioral function after SCI in rats.

11.
Nurs Open ; 2022 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-36403127

RESUMO

AIM: The purpose of this study is to explore the influence of self-management intervention on four prognostic indicators of readmission rate, mortality rate, self-management ability and quality of life in patients with chronic heart failure. DESIGN: A meta-analysis. METHODS: This study was selected from the related studies published from January 1999 to January 2022, and was searched by searching five databases: PubMed, Science of Website, China National Knowledge Infrastructure (CNKI), Wan Fang and Wei Pu (VIP). All standardized randomized controlled trial studies were collected, and the quality evaluation and meta-analysis of the included literature were conducted. RESULTS: This study included 20 randomized controlled trials involving 3459 patients with chronic heart failure. Meta-analysis results showed that self-management intervention could reduce the readmission rate of patients with chronic heart failure, improved self-management ability of patients, improved quality of life, but there was no statistical significance in mortality.

12.
Small ; : e2204738, 2022 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-36403218

RESUMO

Compared to platinum catalysts, ruthenium (Ru) is disclosed as a promising alternative for alkaline water electrolysis due to its similar hydrogen adsorption energy and relatively lower water dissociation barrier. However, in the challenging alkaline media, the dissatisfied Volmer step during water dissociation of Ru metal prohibits its practical applications. Here, a new pathway to modulate the electronic environment of Ru catalysts via a local charge transfer strategy for tuning the water dissociation kinetics and accelerating the alkaline water electrolysis is proposed. The obtained catalysts are engineered by assembling and subsequently pyrolyzing the layer-stacked and 2D porphyrin-based Ru-N coordination polymers on nanocarbon supports. Benefiting from the well-defined Ru nanocluster-Nx -coordination bonds (Runc -Nx ), unique electronic environments, and local charge transfer properties, the catalysts exhibit the exceptional activity of 17 mV overpotential at 10 mA cm-2 and robust stability in water, which is more efficient than state-of-the-art Ru catalysts. The theoretical calculation suggests that the Runc -Nx sites enhance the nucleophilic attack of water and weaken the HOH bond. This study manifests that tailoring the bond environments of Ru clusters can significantly modulate their intrinsic catalytic activities and stabilities, which may open new avenues for developing high-active and durable catalysts for water electrolysis.

13.
Food Chem ; 405(Pt A): 134850, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36368100

RESUMO

Extruded glutelin/rice starch composites were prepared using twin-screw extrusion at various specific mechanical energies (SME), and the interaction mechanism of glutelin and rice starch was investigated at the molecular level. The results indicated that the structure of glutelin was destroyed, hydrophobic interactions and hydrogen bonds between rice starch and glutelin were formed and enhanced as the SME increased, and new hydrogen bonds were formed at the carbonyl (δ- and γ-carbons of glutelin) and C-1 of Tyr. Molecular docking studies confirmed that SME promoted the simultaneous occurrence of the Millard reaction and non-covalent reaction between glutelin and small molecular sugars produced by starch degradation, providing information on binding sites. Additionally, scanning electron microscopy (SEM) revealed dense and uniform flake-like structures induced by these binding interactions. Overall, insights into the interaction mechanism of rice starch and glutelin provide theoretical references for generating reconstituted rice products using extrusion processing.

14.
BMC Med ; 20(1): 437, 2022 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-36352434

RESUMO

BACKGROUND: Cervical squamous cell carcinoma (SCC) is known to arise through increasingly higher-grade squamous intraepithelial lesions (SILs) or cervical intraepithelial neoplasias (CINs). This study aimed to describe sequential molecular changes and identify biomarkers in cervical malignant transformation. METHODS: Multidimensional data from five publicly available microarray and TCGA-CESC datasets were analyzed. Immunohistochemistry was carried out on 354 cervical tissues (42 normal, 62 CIN1, 26 CIN2, 47 CIN3, and 177 SCC) to determine the potential diagnostic and prognostic value of identified biomarkers. RESULTS: We demonstrated that normal epithelium and SILs presented higher molecular homogeneity than SCC. Genes in the region (e.g., 3q, 12q13) with copy number alteration or HPV integration were more likely to lose or gain expression. The IL-17 signaling pathway was enriched throughout disease progression with downregulation of IL17C and decreased Th17 cells at late stage. Furthermore, we identified AURKA, TOP2A, RFC4, and CEP55 as potential causative genes gradually upregulated during the normal-SILs-SCC transition. For detecting high-grade SIL (HSIL), TOP2A and RFC4 showed balanced sensitivity (both 88.2%) and specificity (87.1 and 90.1%), with high AUC (0.88 and 0.89). They had equivalent diagnostic performance alone to the combination of p16INK4a and Ki-67. Meanwhile, increased expression of RFC4 significantly and independently predicted favorable outcomes in multi-institutional cohorts of SCC patients. CONCLUSIONS: Our comprehensive study of gene expression profiling has identified dysregulated genes and biological processes during cervical carcinogenesis. RFC4 is proposed as a novel surrogate biomarker for determining HSIL and HSIL+, and an independent prognostic biomarker for SCC.


Assuntos
Neoplasia Intraepitelial Cervical , Infecções por Papillomavirus , Neoplasias do Colo do Útero , Feminino , Humanos , Prognóstico , Biomarcadores Tumorais/metabolismo , Neoplasia Intraepitelial Cervical/diagnóstico , Neoplasia Intraepitelial Cervical/genética , Neoplasia Intraepitelial Cervical/patologia , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia , Expressão Gênica , Infecções por Papillomavirus/diagnóstico , Infecções por Papillomavirus/genética , Proteína de Replicação C/genética , Proteína de Replicação C/metabolismo , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo
15.
Radiat Res ; 198(5): 488-507, 2022 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-36351324

RESUMO

The intestinal compensatory proliferative potential is a key influencing factor for susceptibility to radiation-induced intestinal injury. Studies indicated that the carnitine palmitoyltransferase 1 (CPT1) mediated fatty acid ß-oxidation (FAO) plays a crucial role in promoting the survival and proliferation of tumor cells. Here, we aimed to explore the effect of 60Co gamma rays on CPT1 mediated FAO in the radiation-induced intestinal injury models, and investigate the role of CPT1 mediated FAO in the survival and proliferation of intestinal cells after irradiation. We detected the changed of FAO in the plasma and small intestine of Sprague Dawley (SD) rats at 24 h after 60Co gamma irradiation (0, 5 and 10 Gy), using target metabolomics, qRT-PCR, immunohistochemistry (IHC), western blot (WB) and related enzymatic activity kits. We then analyzed the FAO changes in radiation-induced intestinal injury models regardless of ex vivo (mice enteroids), or in vitro (normal human intestinal epithelial cell lines, HIEC-6). HIEC-6 cells were transduced with lentivirus vector GV392 and treated with puromycin for obtaining CPT1 stable knockout cell lines, named CPT1 KO. CPT1 enzymatic activities of HIEC-6 cells and mice enteroids were also inhibited by pharmaceutical inhibitor ST1326 and Etomoxir (ETO), to study the function of CPT1 in the survival and proliferation of HIEC-6 cells after 60Co gamma irradiation. We found that CPT1 mediated FAO was altered in the small intestine of the SD rats after irradiation, especially, the expression level and enzymatic activity of CPT1 were significantly increased. Similarly, the expression levels of CPT1 were also remarkably enhanced in mice enteroids and HIEC-6 cells after irradiation. CPT1 inhibition decreased the proliferation of the HIEC-6 cells and mice enteroids after irradiation partially by reducing the extracellular signal-regulated kinase (ERK1/2) and c-Jun N-terminal kinase (JNK) pathways activation, CPT1 inhibition also reduced the proliferation of mice enteroids after irradiation partially by down-regulating the Wnt/ß-catenin signaling activity. In conclusion, our study indicated that CPT1 plays a crucial role in promoting intestinal epithelial cell proliferation after irradiation.


Assuntos
Carnitina O-Palmitoiltransferase , Sistema de Sinalização das MAP Quinases , Animais , Humanos , Camundongos , Ratos , Carnitina O-Palmitoiltransferase/genética , Carnitina O-Palmitoiltransferase/metabolismo , Proliferação de Células , Raios gama , Ratos Sprague-Dawley , Oxirredução
16.
Nat Commun ; 13(1): 6817, 2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36357398

RESUMO

A four-year-old boy developed recurrent fever and severe pneumonia in April, 2022. High-throughput sequencing revealed a reassortant avian influenza A-H3N8 virus (A/Henan/ZMD-22-2/2022(H3N8) with avian-origin HA and NA genes. The six internal genes were acquired from Eurasian lineage H9N2 viruses. Molecular substitutions analysis revealed the haemagglutin retained avian-like receptor binding specificity but that PB2 genes possessed sequence changes (E627K) associated with increased virulence and transmissibility in mammalian animal models. The patient developed respiratory failure, liver, renal, coagulation dysfunction and sepsis. Endotracheal intubation and extracorporeal membrane oxygenation were administered. H3N8 RNA was detected from nasopharyngeal swab of a dog, anal swab of a cat, and environmental samples collected in the patient's house. The full-length HA sequences from the dog and cat were identical to the sequence from the patient. No influenza-like illness was developed and no H3N8 RNA was identified in family members. Serological testing revealed neutralizing antibody response against ZMD-22-2 virus in the patient and three family members. Our results suggest that a triple reassortant H3N8 caused severe human disease. There is some evidence of mammalian adaptation, possible via an intermediary mammalian species, but no evidence of person-to-person transmission. The potential threat from avian influenza viruses warrants continuous evaluation and mitigation.


Assuntos
Doenças do Gato , Doenças do Cão , Vírus da Influenza A Subtipo H3N8 , Vírus da Influenza A Subtipo H9N2 , Influenza Aviária , Influenza Humana , Infecções por Orthomyxoviridae , Masculino , Humanos , Cães , Animais , Gatos , Pré-Escolar , Vírus da Influenza A Subtipo H3N8/genética , Vírus da Influenza A Subtipo H9N2/genética , Aves/genética , RNA , Filogenia , Vírus Reordenados/genética , Influenza Humana/epidemiologia , Mamíferos/genética
17.
Foods ; 11(21)2022 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-36359935

RESUMO

This study aimed to compare tilapia fish cake drying and sterilization conditions (105, 115, and 121 °C) on the quality of the cakes. The impacts of volatile flavor substances, the chroma value, quality and structure characteristics, microscopic structure, and the types and content of volatile flavor substances were also analyzed. The results showed that after drying and sterilization, the L* value, W value and delta-E value of fish cakes decreased significantly from 77.12 to 64.77, 66.21 to 52.57, 10.46 to 24.50, respectively. However, a* value and b* value increased significantly from 0.30 to 6.97 and 24.85 to 30.89, respectively. The elasticity, hardness, and chewiness increased significantly with the drying process but decreased significantly with the increased sterilization temperature. Scanning electron microscopy results showed that the internal pores of the fish cakes became smaller, and the tissue structure was closer after drying. Gas chromatography-ion mobile spectrometry analysis identified a total of 36 volatile flavor compounds. Among these, ketones comprised the largest content, aldehydes represented the largest variety, and all volatile compounds contributed significantly to the flavor of fish cake. PCA results and nearest-neighbor fingerprint analysis showed that there were obvious differences in volatile flavor compounds between different treatments. In summary, this study conducted a detailed comparative analysis of the quality and flavor of fish cakes subjected to different processing methods. These findings contribute suggestions for sterilization temperatures in industrial production processes.

18.
Artigo em Inglês | MEDLINE | ID: mdl-36374375

RESUMO

PURPOSE: To explore the correlation between shear-wave elastography (SWE) parameters and pathological profiles of invasive breast cancer. METHODS: A total of 197 invasive breast cancers undergoing preoperative SWE and primary surgical treatment were included. Maximum elastic modulus (Emax), mean elastic modulus (Emean), and elastic modulus standard deviation (Esd) were calculated by SWE. Pathological profile was gold standard according to postoperative pathology. The relationship between SWE parameters and pathological factors were analyzed using univariate and multivariate analysis. RESULTS: In univariate analysis, large cancers showed significantly higher Emax, Emean and Esd (all P < 0.001). Emax and Esd in the group of histological grade III were higher than those in the group of grade I (both P < 0.05). Invasive lobular carcinomas (ILC) showed higher Emean than invasive ductal carcinoma (IDC) (P < 0.001). Lymphovascular invasion (LVI) group showed higher Emax values than negative group (P < 0.05). Emax, Emean and Esd of the Ki-67 positive group presented higher values than negative group (all P < 0.05). Androgen receptor (AR) positive lesions had lower Esd than AR negative lesions (P < 0.05). In multivariate analysis, invasive size independently influenced Emax (P < 0.001). Invasive size and pathological type both independently influenced Emean (both P < 0.001). Invasive size and AR status were both independently influenced Esd (both P < 0.05). CONCLUSION: SWE parameters correlated with pathological profiles of invasive breast cancer.In particular, AR positive group showed significantly low Esd than negative group.

19.
Artigo em Inglês | MEDLINE | ID: mdl-36374383

RESUMO

The urgent global climate change situation forces the steel industry to confront enormous challenges and complex tasks. This research focuses on the steel sector and incorporates data from 56 nations between 2010 and 2018. We analyze the direct and indirect effects of the steel trade on CO2 emissions using the spatial Durbin model. The results show that Moran's I ranges from 0.414 to 0.521, suggesting that carbon emissions are very spatially dependent. Developed countries such as Japan, Germany, and the USA form high-high agglomeration areas. In contrast, Brazil, Thailand, and India constitute low-low agglomeration areas. Under the three spatial weight matrices, the direct effect coefficients of steel exports are 0.045, 0.038, and 0.057, and the indirect effect coefficients are - 0.006, - 0.076, and - 0.015, indicating that steel exports increase local CO2 emissions while decreasing carbon emissions in neighboring countries. Urbanization and per capita GDP have positive spatial spillover effects, while the spatial effect of R&D intensity is insignificant. Increased industrialization and renewable energy consumption positively affect carbon emission reduction in local and surrounding nations. The study provides empirical evidence for the steel industry to develop emission reduction strategies.

20.
Bioresour Technol ; 366: 128210, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36323372

RESUMO

In this study, the metabolic fates and response strategies of microorganisms to aromatic compounds with different structures (phenol, naphthalene, phenanthrene, and pyrene) were comparatively studied. The results indicated that the phenol (90.9%), naphthalene (68.4%), phenanthrene (69.5%), and pyrene (67.1%) could be mineralized, and the biotoxicity also has been drastically reduced. The degradation characteristics and toxic effects were closely related to their chemical structure. The microorganisms showed different response strategies to aromatic compounds with different structures. Phenol had a simple structure and low toxicity, the microbial community structure was simple, and the rapid expression of key enzymes enabled it to be rapidly degraded. For the hydrophobic and complex naphthalene, phenanthrene, and pyrene, the more complex the structure, the higher the microbial diversity, the EPS showed different response for the purpose of improving their bioavailability, and the activity of key enzymes was positively correlated with their structural complexity.


Assuntos
Fenantrenos , Hidrocarbonetos Policíclicos Aromáticos , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Biodegradação Ambiental , Pirenos , Fenantrenos/metabolismo , Compostos Orgânicos , Naftalenos , Fenol
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