Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 184
Filtrar
1.
Neoplasma ; 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33231089

RESUMO

Cancer-associated fibroblasts (CAFs) are the major constituents of the tumor microenvironment and promote cancer development via tumor-stromal interactions. The alteration of microRNA (miRNA) expression in fibroblasts can induce the phenotype conversion between normal fibroblasts and CAFs in certain tumor types. However, the mechanisms underlying phenotype conversion of fibroblasts in colorectal cancer (CRC) are largely unknown. Our study focuses on the role of miR-1246 in fibroblasts-CRC cells interaction. In this study, CCD-18Co colorectal fibroblasts were cultured in the conditioned medium (CM) derived from CRC cells to obtain the CAF phenotype. We found that the miR-1246 expression was upregulated in CAF-like fibroblasts compared with normal fibroblasts. miR-1246 secreted by cancer cells could be utilized by neighboring fibroblasts for CAF reprogramming. On the other hand, following secretion by CAF-like fibroblasts, miR-1246 was delivered into CRC cells and promoted cell migration via the activation of the Wnt/ß-catenin signaling in CRC cells. Furthermore, high miR-1246 expression in CRC tissues was negatively associated with disease-free survival (DFS) for CRC patients. Taken together, our results reveal that miR-1246 can shuttle between CRC cells and fibroblasts. This study also indicates that targeting miR-1246 or blocking its transport from CAFs to CRC cells might represent a novel therapeutic approach in CRC treatment.

2.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(8): 922-927, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32912403

RESUMO

OBJECTIVE: To evaluate the clinical effect of integrated traditional Chinese and Western medicine on common type of coronavirus disease 2019 (COVID-19) in Henan Province. METHODS: A prospective single arm clinical study was performed. Patients with common type of COVID-19 admitted to seven designated hospitals for COVID-19 in Henan Province from January 25th to February 26th, 2020 were enrolled, and treated with integrated traditional Chinese and Western medicine. The negative transformation of 2019 novel coronavirus (2019-nCoV) nucleic acid, disease outcome, hospital stay, clinical symptoms and signs scores, and chest imaging performance were observed. RESULTS: Totally 86 cases were included in the analysis, including 48 males (55.8%), aged 43.5 (35.0, 53.3) years old, 24 patients (27.9%) with previous medical history. Fifty-eight patients were primarily diagnosed COVID-19 and 28 patients were transferred. The 2019-nCoV nucleic acid of 86 cases (100%) turned negative, and the median time of turning negative was 10 (7, 14) days. Eighty-six cases (100%) were discharged from hospital, and none turned into the severe type; the average length of hospital stay was (13.8±5.6) days. The scores of fever, cough, chest tightness, shortness of breath, and fatigue decreased with the treatment time, and the scores of 7 days and 14 days after treatment were significantly lower than those before treatment [fever (points): 0 (0, 0), 0 (0, 0) vs. 1 (0, 1); cough (points): 1 (0, 1), 0 (0, 1) vs. 1 (0, 2); chest tightness (points): 0 (0, 0), 0 (0, 0) vs. 0 (0, 1); shortness of breath (points): 0 (0, 0), 0 (0, 0) vs. 0 (0, 1); fatigue (points): 0 (0, 1), 0 (0, 1) vs. 1 (0, 1); all P < 0.05]. The improvement rate of X ray and CT image was 42.9% (12/28) and 81.0% (64/79), respectively. CONCLUSIONS: The treatment with integrated traditional Chinese and Western medicine has good curative effect on common type of COVID-19 in 7 designated hospitals of Henan Province. It can improve the clinical symptoms, promote the absorption of pulmonary inflammation, and to some extent control the progress of disease and shorten the time of turning negative of virus nucleic acid and hospital stay.


Assuntos
Infecções por Coronavirus/terapia , Medicina Integrativa , Medicina Tradicional Chinesa/métodos , Pneumonia Viral/terapia , Adulto , China/epidemiologia , Terapia Combinada , Infecções por Coronavirus/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/epidemiologia , Estudos Prospectivos , Resultado do Tratamento
3.
Eur J Cancer Prev ; 29(5): 424-432, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32740168

RESUMO

OBJECTIVES: Results from epidemiologic studies on age at last birth (ALB) and the risk of developing breast cancer were inconsistent. Therefore, we conducted this meta-analysis to evaluate the association between ALB and the risk of developing breast cancer quantitatively. METHODS: Relevant articles published up to May 2019 were identified by searching systematically in PubMed, Web of Science, China National Knowledge Infrastructure, and Wan Fang Med Online. Pooled relative risks (RRs) with 95% confidence intervals (CIs) were calculated using a random-effects model. Dose-response relationship was assessed by restricted cubic spline model. RESULTS: Fourteen studies with 132 133 cases and 1 314 005 participants were eligible for this meta-analysis. The pooled RR (95% CI) of breast cancer for the highest vs. the lowest stratification of ALB was 1.22 (1.04-1.42). In the subgroup analysis, significant positive associations were also found in studies conducted in Europe (RR: 1.21, 95% CI: 1.06-1.38), studies with results adjusted for parity (RR: 1.26, 95% CI: 1.04-1.54), and studies with results adjusted for age at first birth (RR: 1.37, 95% CI: 1.08-1.74). The results of the dose-response analysis indicated that the departure from linearity was NS between ALB and the risk of breast cancer (Pnonlinearity = 0.711), but the linear associations were NS. CONCLUSION: This meta-analysis suggested that ALB was positively associated with the risk of breast cancer. The risk of developing breast cancer increased gradually with the ALB for women. Our findings may have implications for family planning.

4.
Curr Med Sci ; 40(4): 625-635, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32767264

RESUMO

Nurses' work-related fatigue has been recognized as a threat to nurse health and patient safety. The aim of this study was to assess the prevalence of fatigue among first-line nurses combating with COVID-19 in Wuhan, China, and to analyze its influencing factors on fatigue. A multi-center, descriptive, cross-sectional design with a convenience sample was used. The statistical population consisted of the first-line nurses in 7 tertiary general hospitals from March 3, 2020 to March 10, 2020 in Wuhan of China. A total of 2667 samples from 2768 contacted participants completed the investgation, with a response rate of 96.35%. Social-demographic questionnaire, work-related questionnaire, Fatigue Scale-14, Generalized Anxiety Disorder-7, Patient Health Questionnaire-9, and Chinese Perceived Stress Scale were used to conduct online survey. The descriptive statistic of nurses' social-demographic characteristics was conducted, and the related variables of work, anxiety, depression, perceived stress and fatigue were analyzed by t-tests, nonparametric test and Pearson's correlation analysis. The significant factors which resulted in nurses' fatigue were further analyzed by multiple linear regression analysis. The median score for the first-line nurses' fatigue in Wuhan was 4 (2, 8). The median score of physical and mental fatigue of them was 3 (1, 6) and 1 (0, 3) respectively. According to the scoring criteria, 35.06% nurses (n=935) of all participants were in the fatigue status, their median score of fatigue was 10 (8, 11), and the median score of physical and mental fatigue of them was 7 (5, 8) and 3 (2, 4) respectively. Multiple linear regression analysis revealed the participants in the risk groups of anxiety, depression and perceived stress had higher scores on physical and mental fatigue and the statistically significant positive correlation was observed between the variables and nurses' fatigue, the frequency of exercise and nurses' fatigue had a statistically significant negative correlation, and average daily working hours had a significantly positive correlation with nurses' fatigue, and the frequency of weekly night shift had a low positive correlation with nurses' fatigue (P<0.01). There was a moderate level of fatigue among the first-line nurses fighting against COVID-19 pandemic in Wuhan, China. Government and health authorities need to formulate and take effective intervention strategies according to the relevant risk factors, and undertake preventive measures aimed at reducing health hazards due to increased work-related fatigue among first-line nurses, and to enhance their health status and provide a safe occupational environment worldwide. Promoting both medical and nursing safety while combating with the pandemic currently is warranted.


Assuntos
Betacoronavirus , Infecções por Coronavirus/enfermagem , Fadiga/etiologia , Enfermeiras e Enfermeiros , Doenças Profissionais/etiologia , Estresse Ocupacional/etiologia , Pandemias , Pneumonia Viral/enfermagem , Adulto , China/epidemiologia , Infecções por Coronavirus/epidemiologia , Estudos Transversais , Fadiga/epidemiologia , Fadiga/psicologia , Feminino , Humanos , Modelos Lineares , Masculino , Fadiga Mental/epidemiologia , Fadiga Mental/etiologia , Fadiga Mental/psicologia , Pessoa de Meia-Idade , Enfermeiras e Enfermeiros/psicologia , Doenças Profissionais/epidemiologia , Doenças Profissionais/psicologia , Estresse Ocupacional/epidemiologia , Estresse Ocupacional/psicologia , Pneumonia Viral/epidemiologia , Prevalência , Fatores de Risco , Inquéritos e Questionários , Centros de Atenção Terciária , Carga de Trabalho/psicologia , Adulto Jovem
5.
Arch Gerontol Geriatr ; 90: 104129, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32502885

RESUMO

BACKGROUND/OBJECTIVE: Previous studies have linked lung function to cognitive performance. However, it is not clear whether baseline lung function has an effect on the trajectory of cognitive decline during normal aging. This study aimed to examine the association of baseline lung function with long-term changes in cognition among the middle-aged and older adults. METHODS: Lung function as indicated by forced expiratory volume 1 s (FEV1) and forced vital capacity (FVC), was measured at the baseline examination. Cognition, including memory, time orientation, executive function and processing speed, were tested four times over six years. Generalized estimating equation (GEE) models were used to test the associations between baseline lung function and four visits of cognition in 6080 participants aged 50 years or over from the English Longitudinal Study of Ageing (ELSA). RESULTS: Compared to participants with higher lung function, those who had lower lung function at baseline experienced a faster rate of decline in memory (joint test: χ2interaction = 12.07, df = 3, P = 0.007 for FVC), time orientation (joint test: χ2interaction = 9.49, df = 3, P = 0.023 for FVC) and executive function (joint test: χ2interaction = 9.13, df = 3, P = 0.028 for FEV1 and joint test: χ2interaction = 12.76, df = 3, P = 0.005 for FVC). No association was found between baseline lung function and the rate of decline in processing speed (joint test: χ2interaction = 1.29, df = 3, P=0.733 for FEV1 and joint test: χ2interaction = 2.35, df = 3, P = 0.503 for FVC). CONCLUSIONS: Poor lung function at baseline predicted a faster rate of cognitive decline in memory, time orientation and executive function. The mechanism for this association deserves further investigation.

6.
Clin Lab ; 66(5)2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32390398

RESUMO

BACKGROUND: Pompe disease is an inherited neuromuscular disease caused by a deficiency of lysosomal acid alphaglucosidase (GAA) leading to glycogen accumulation in muscle and motor neurons. METHODS: We retrospectively collected the clinical information on the case of a 2-year-old child admitted to the emergency department with respiratory failure and generalized hypotonia. RESULTS: Chest radiograph, cerebral and spinal magnetic resonance imaging, muscle biopsy, etc. were all examined. The 2-year-old child was later diagnosed with Pompe disease. Systematic assessment and effective sedation management were applied. CONCLUSIONS: Differential diagnosis, systematic assessment, and effective sedation management are important for Pompe disease patients.

7.
Inflammation ; 43(4): 1423-1435, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32388657

RESUMO

Ulcerative colitis (UC) is a chronic and relapsing inflammatory disorder of the gastrointestinal tract, characterized by diarrhea, hematochezia, abdominal distension, and abdominal pain. The perpetuation of inflammation and the impairment of the intestinal barrier are part of the main courses of UC, responsible for the deteriorating inflammatory condition. Patchouli alcohol (PA), extracted from Pogostemon cablin Benth., is employed to treat both inflammation and intestinal barrier damage. Its curative effect on UC was testified firstly by TNBS-induced UC, a chemically induced colitis, and further tested by DSS-induced UC, an acute attack stage of UC in which the clinical course of human UC occurs frequently. PA reduced the levels of TNF-α, IFN-γ, IL-1ß, IL-6, and IL-17 in serum and decreased the mRNA expression of pro-inflammatory cytokines (e.g., iNOS, COX-2, TNF-α, IL-1ß, and IL-6). Concurrently, PA upregulated the expression of tight junction protein (e.g., ZO-1, ZO-2, claudin-1, and occludin) and the mRNA of mucin-1 and mucin-2 in both animal models. Further, PA ameliorated both histological damage and clinical parameters. Thus, PA could credibly reduce the expression of pro-inflammatory cytokines, protect the integrity of intestinal epithelial barrier, and repair the macroscopic colon lesions in both colitis models.

8.
J Toxicol Sci ; 45(5): 281-291, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32404560

RESUMO

Despite the developmental toxicity reported in animals, few epidemiologic studies have investigated the potential effects of prenatal exposure to pyrethroid pesticides (PYRs) on fetal growth. A birth cohort study was conducted to examine the association between prenatal exposure to PYRs and birth outcomes, and a nested case-control study was conducted in this cohort to evaluate the effects of PYR on congenital defects. The assessment of PYR exposure was based on self-reported household pesticide use and urinary PYR metabolite levels. We found that pregnant women in this region were ubiquitously exposed to low-level PYRs, although few reported household pesticide use. Women who often ate bananas or cantaloupes had a higher level of urinary 3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DBCA), and the number of fruit types consumed by pregnant women was positively related to the concentrations of 3-phenoxybenzoic acid (3PBA) and total PYR metabolites (P < 0.05). Increased urinary 4-fluoro-3-phenoxybenzoic acid (4F3PBA), DBCA, and total PYR metabolites were associated with increased birth weight, length, and gestational age, and with decreased risk of small for gestational age (SGA) and/or premature birth. However, maternal household pesticides use was related to congenital anomalies. Thus, although prenatal exposure to low-dose PYRs promoted the fetal growth, the beneficial effects of fruit intake may outweigh the adverse effects of pesticide exposure. This study provided us an insight into the biological mechanisms for the effect of prenatal PYR exposure on fetal development, and suggested that further investigations in a larger study population with low-dose PYR exposure is needed.


Assuntos
Desenvolvimento Fetal/efeitos dos fármacos , Exposição Materna/efeitos adversos , Praguicidas/efeitos adversos , Piretrinas/efeitos adversos , Anormalidades Induzidas por Medicamentos/etiologia , Adulto , Estudos de Coortes , Feminino , Humanos , Troca Materno-Fetal , Praguicidas/metabolismo , Gravidez , Resultado da Gravidez , Piretrinas/metabolismo , Piretrinas/urina , Adulto Jovem
9.
Br J Nutr ; 123(11): 1290-1301, 2020 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-32077385

RESUMO

Depression is an important public health problem. The aim of the study is to explore the associations of total protein intake and protein sources with the risk of depressive symptoms. This cross-sectional study used data from the National Health and Nutrition Examination Survey for the years 2007-2014. Dietary protein intake was obtained from two 24-h dietary recall interviews. Depressive symptoms were assessed by a nine-item Patient Health Questionnaire. Logistic regression models and restricted cubic spline models were used to estimate the associations of total protein intake (g/kg per d) and protein sources with the risk of depressive symptoms. A total of 17 845 individuals aged 18 years and older were included in this study. Total protein intake was inversely associated with the risk of depressive symptoms. The full-adjusted OR of depressive symptoms was 0·34 (95 % CI 0·17, 0·68) for quartile (Q) 4 v. Q1 of total protein intake. For protein intake from milk and milk products, the association with depressive symptoms was significant both for Q2 v. Q1 (OR 0·61; 95 % CI 0·41, 0·93) and Q3 v. Q1 (OR 0·37; 95 % CI 0·24, 0·59) in the full-adjusted model. In the dose-response analysis, the shape of the associations of total protein intake and protein intake from milk and milk products with the risk of depressive symptoms was approximately L-shaped and U-shaped, respectively. The present study demonstrated that total protein intake and protein intake from milk and milk products might reduce the risk of depressive symptoms in US adults.

10.
Int J Food Sci Nutr ; 71(6): 738-749, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31986943

RESUMO

The study used the data from the National Health and Nutrition Examination Survey (NHANES) 2007-2014 to analyse the relationship of dietary vitamin B1, B2, niacin, B6, B12 and dietary folate equivalent (DEF) intakes with metabolic syndrome. In the multivariate-adjusted model 2, compared with the lowest quartile of dietary intake, the odd ratios (ORs;95% confidence intervals (CIs)) were 0.73 (0.59-0.91), 0.76 (0.61-0.95), 0.76 (0.59-0.98) and 0.77 (0.62-0.96) for the highest quartile of vitamin B1, niacin, B6 and DFE, respectively. The ORs (95%CIs) for the third and the highest quartile of vitamin B2 were 0.78 (0.61-0.99) and 0.62 (0.47-0.83). A linear inverse relationship was found between dietary vitamin B1, niacin, B6, DFE and metabolic syndrome, and a non-linear inverse relationship was found between dietary vitamin B2 and metabolic syndrome. Our results suggested that higher intake of vitamin B1, B2, niacin, B6 and DFE were all associated with reduced risk of metabolic syndrome.

11.
Clin Chim Acta ; 503: 19-34, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31923423

RESUMO

Hypertriglyceridemia is a risk factor for a series of diseases, such as cardiovascular disease (CVD), diabetes and nonalcoholic fatty liver disease (NAFLD). Angiopoietin-like proteins (ANGPTLs) family, especially ANGPTL3, ANGPTL4 and ANGPTL8, which regulate lipoprotein lipase (LPL) activity, play pivotal roles in triglyceride (TG) metabolism and related diseases/complications. There are many transcriptional and post-transcriptional factors that participate in physiological and pathological regulation of ANGPTLs to affect triglyceride metabolism. This review is intended to focus on the similarity and difference in the expression, structural features, regulation profile of the three ANGPTLs and inhibitory models for LPL. Description of the regulatory factors of ANGPTLs and the properties in regulating the lipid metabolism involved in the underlying mechanisms in pathological effects on diseases will provide potential therapeutic approaches for the treatment of dyslipidemia related diseases.


Assuntos
Proteínas Semelhantes a Angiopoietina/fisiologia , Lipase Lipoproteica/antagonistas & inibidores , Triglicerídeos/metabolismo , Proteína 4 Semelhante a Angiopoietina , Animais , Humanos , Lipase Lipoproteica/metabolismo , Hormônios Peptídicos
12.
World J Gastroenterol ; 25(43): 6416-6429, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31798278

RESUMO

BACKGROUND: Fecal metabolites are associated with gut visceral sensitivity, mucosal immune function and intestinal barrier function, all of which have critical roles in the pathogenesis of irritable bowel syndrome (IBS). However, the metabolic profile and pathophysiology of IBS are still unclear. We hypothesized that altered profiles of fecal metabolites might be involved in the pathogenesis of IBS with predominant diarrhea (IBS-D). AIM: To investigate the fecal metabolite composition and the role of metabolites in IBS-D pathophysiology. METHODS: Thirty IBS-D patients and 15 age- and sex-matched healthy controls (HCs) underwent clinical and psychological assessments, including the IBS Symptom Severity System (IBS-SSS), an Italian modified version of the Bowel Disease Questionnaire, the Bristol Stool Form Scale (BSFS), the Hospital Anxiety and Depression Scale, and the Visceral Sensitivity Index. Visceral sensitivity to rectal distension was tested using high-resolution manometry system by the same investigator. Fecal metabolites, including amino acids and organic acids, were measured by targeted metabolomics approaches. Correlation analyses between these parameters were performed. RESULTS: The patients presented with increased stool water content, more psychological symptoms and increased visceral hypersensitivity compared with the controls. In fecal metabolites, His [IBS-D: 0.0642 (0.0388, 0.1484), HC: 0.2636 (0.0780, 0.3966), P = 0.012], Ala [IBS-D: 0.5095 (0.2826, 0.9183), HC: 1.0118 (0.6135, 1.4335), P = 0.041], Tyr [IBS-D: 0.1024 (0.0173, 0.4527), HC: 0.5665 (0.2436, 1.3447), P = 0.018], Phe [IBS-D: 0.1511 (0.0775, 0.3248), HC: 0.3967 (0.1388, 0.7550), P = 0.028], and Trp [IBS-D: 0.0323 (0.0001, 0.0826), HC: 0.0834 (0.0170, 0.1759), P = 0.046] were decreased in IBS-D patients, but isohexanoate [IBS-D: 0.0127 (0.0060, 0.0246), HC: 0.0070 (0.0023, 0.0106), P = 0.028] was significantly increased. Only Tyr was mildly correlated with BSFS scores in all subjects (r = -0.347, P = 0.019). A possible potential biomarker panel was identified to correlate with IBS-SSS score (R 2 Adjusted = 0.693, P < 0.001). In this regression model, the levels of Tyr, Val, hexanoate, fumarate, and pyruvate were significantly associated with the symptom severity of IBS-D. Furthermore, visceral sensation, including abdominal pain and visceral hypersensitivity, was correlated with isovalerate, valerate and isohexanoate. CONCLUSION: Altered profiles of fecal metabolites may be one of the origins or exacerbating factors of symptoms in IBS-D via increasing visceral sensitivity.


Assuntos
Dor Abdominal/etiologia , Ácidos Carboxílicos/metabolismo , Síndrome do Intestino Irritável/metabolismo , Dor Abdominal/metabolismo , Adulto , Ácidos Carboxílicos/análise , Estudos de Casos e Controles , Fezes/química , Feminino , Humanos , Síndrome do Intestino Irritável/complicações , Masculino , Índice de Gravidade de Doença
13.
Trials ; 20(1): 735, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842969

RESUMO

BACKGROUND: The incidence, mortality, and prevalence of chronic obstructive pulmonary disease (COPD) are high in China. Acute exacerbations of COPD (AECOPD) are important events in the management of COPD because they negatively impact health status, rates of hospitalization and readmission, and disease progression. AECOPD have been effectively treated with Chinese medicine for a long time. The aim of this proposed trial is to assess the therapeutic effect of Chinese medicine (CM) on AECOPD. METHODS/DESIGN: This proposed study is a multicenter, double-blind, parallel-group randomized controlled trial (RCT). We will randomly assign 378 participants with AECOPD into two groups in a 1:1 ratio. On the basis of health education and conventional treatment, the intervention group will be treated with CM, and the control group is given CM placebo according to CM syndrome. Patients are randomized to either receive CM or placebo, 10 g/packet, twice daily. The double-blind treatment lasts for 2 weeks and is followed up for 4 weeks. The main outcome is the COPD Assessment Test; secondary outcomes are treatment failure rate, treatment success rate, length of hospital stay, AECOPD readmission rate, intubation rate, mortality, dyspnea, the 36-item Short Form Health Survey, and the COPD patient-reported outcome scale. We will document these outcomes faithfully at the beginning of the study, 2 weeks after treatment, and at the 4 weeks follow-up. DISCUSSION: This high-quality RCT with strict methodology and few design deficits will help to prove the effectiveness of CM for AECOPD. We hope this trial will provide useful evidence for developing a therapeutic schedule with CM for patients with AECOPD. TRIAL REGISTRATION: ClinicalTrials.gov, NCT03428412. Registered on 4 February 2018.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Pulmão/efeitos dos fármacos , Doença Pulmonar Obstrutiva Crônica/tratamento farmacológico , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Progressão da Doença , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Estudos de Equivalência como Asunto , Feminino , Humanos , Pulmão/fisiopatologia , Masculino , Pessoa de Meia-Idade , Estudos Multicêntricos como Assunto , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Fatores de Tempo , Resultado do Tratamento
14.
J Alzheimers Dis ; 72(4): 1145-1157, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31683474

RESUMO

BACKGROUND: The association of zinc, iron, copper, and selenium intakes with cognitive function is poorly understood so far. OBJECTIVE: To examine the associations of dietary and total zinc, iron, copper, and selenium intakes with low cognitive performance. METHODS: Cross-sectional study data from the National Health and Nutrition Examination Survey (NHANES) 2011-2014 was used. Zinc, iron, copper, and selenium intakes from foods and supplements were estimated from two non-consecutive 24-hour diet recalls. Cognitive function was measured by the Consortium to Establish a Registry for Alzheimer's disease (CERAD) Word Learning sub-test, Animal Fluency test, and Digit Symbol Substitution test (DSST). For each cognitive measurement, people whose score were lower than the age group stratified lowest quartile were defined as low cognitive performance. Logistic regression and restricted cubic spline models were applied to examine the associations of dietary and total zinc, iron, copper, and selenium intakes with different measures of low cognitive performance. RESULTS: A total of 2,332 adults aged 60 years or older were included. The association between zinc, iron, copper, and selenium intake and low cognitive performance was significant in different test. Compared with the lowest quartile of total copper intake, the weighted multivariate adjusted ORs (95% CI) of the highest quartile were 0.34 (0.16-0.75) for low cognitive performance in DSST. L-shaped associations between total copper or selenium and low cognitive performance in DSST and animal fluency were found. CONCLUSION: Dietary and total zinc, copper, and selenium intakes might be inversely associated with the prevalence of low cognitive performance.

15.
Proc Inst Mech Eng H ; 233(12): 1310-1317, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31617820

RESUMO

This study aimed to evaluate the safety and accuracy of mixed reality-based intraoperative three-dimensional navigated pedicle screws in three-dimensional printed model of fractured upper cervical spine. A total of 27 cervical model from patients of upper cervical spine fractures formed the study group. All the C1 and C2 pedicle screws were inserted under mixed reality-based intraoperative three-dimensional image-guided navigation system. The accuracy and safety of the pedicle screw placement were evaluated on the basis of postoperative computerized tomography scans. A total of 108 pedicle screws were properly inserted into the cervical three-dimensional models under mixed reality-based navigation, including 54 C1 pedicle screws and 54 C2 pedicle screws. Analysis of the dimensional parameters of each pedicle at C1/C2 level showed no statistically significant differences in the ideal and the actual entry points, inclined angles, and tailed angles. No screw was misplaced outside the pedicle of the three-dimensional printed model, and no ionizing X-ray radiation was used during screw placement under navigation. It is easy and safe to place C1/C2 pedicle screws under MR surgical navigation. Mixed reality-based navigation is feasible within upper cervical spinal fractures with improved safety and accuracy of C1/C2 pedicle screw insertion.


Assuntos
Imageamento Tridimensional , Parafusos Pediculares , Cirurgia Assistida por Computador/instrumentação , Adulto , Estudos de Viabilidade , Feminino , Humanos , Período Intraoperatório , Masculino , Pessoa de Meia-Idade , Adulto Jovem
16.
J Ethn Subst Abuse ; : 1-13, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31510870

RESUMO

Previous studies have reported controversial results about the association between rs6296 and alcoholism. Thus, a meta-analysis was performed to further explore this association. A comprehensive search was conducted to identify relevant case-control or cohort studies (up to December 1, 2017). A fixed- or random-effect model was selected as a pooling method depending on the heterogeneity among studies. The heterogeneity was measured by Q test and I2 statistic. The Harbord and Peters test was used to estimate publication bias. Fifteen English articles with 16 outcomes and 5,429 participants were included in this meta-analysis. A fixed-effect model was chosen, and the pooled result showed that rs6296 was not related to alcoholism (z = 1.93, p = .053). The Harbord and Peters test showed that there was no publication bias. This meta-analysis indicated that rs6296 may be not be significantly associated with alcoholism, which needs to be further confirmed by future research.

17.
J Am Geriatr Soc ; 67(11): 2331-2337, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31373385

RESUMO

OBJECTIVES: To evaluate the dose-response relationships between alcohol consumption and functional limitations in older European men, and explore the role of muscle strength as a mediator of these relationships. DESIGN: Cross-sectional study of older men participating in the Survey of Health, Aging and Retirement in Europe (SHARE). SETTING: Urban and rural households in 17 European countries and Israel. PARTICIPANTS: A total of 17 870 men aged 65 years and older from the SHARE (Wave 6, 2015) were included in this study. MEASUREMENTS: Outcome variables were functional limitations: mobility limitation, arm function limitation, and fine motor limitation. Main exposure variable was alcohol consumption. Mediating factor was grip strength. Basic demographics, life habits, and health status were considered as potential confounders. Dose-response analyses with restricted cubic splines and the Karlson/Holm/Breen method were conducted. RESULTS: A total of 17 870 participants were included in this study. Dose-response analyses revealed that moderate alcohol consumption was related to the lower odds of reporting mobility limitation (≤35 units/wk) and arm function limitation (≤41 units/wk), with a minimum odds ratio (OR) occurring at 10 units/week drinks for mobility limitation (OR = .71; 95% confidence interval [CI] = .62-.81) and arm function limitation (OR = .66; 95% CI = .59-.75). The odds of reporting the fine motor limitation monotonically increased with alcohol consumption when alcohol consumption was beyond 15 units/week. No significant mediating effect of grip strength on the relationships between alcohol consumption and mobility limitation and arm function limitation was found. CONCLUSION: Moderate alcohol consumption has a protective role in mobility and arm function limitation in older European men. Grip strength is not the main mediator of these associations, suggesting that the protective effect is independent of muscle strength. Alcohol consumption is associated with higher odds of reporting fine motor limitation in older European men. J Am Geriatr Soc 67:2331-2337, 2019.

18.
Front Pharmacol ; 10: 737, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31333459

RESUMO

Chronic obstructive pulmonary disease (COPD) has been a major public health problem and is still a formidable challenge for clinicians. It is urgent to find new compounds for minimizing the risk of disease progression and exacerbation especially in the early phase of COPD. A traditional Chinese medicine (TCM) formula, Chuan Bei Pi Pa dropping pills (CBPP), was tested in this study to investigate its potential mechanisms in preventing the exacerbation of COPD. Phosphoproteomics analysis for a smog stimulated early stage COPD mice model was employed to detect the underlying molecular mechanisms of CBPP. In addition, protein-protein interaction (PPI) and bioinformatics analyses were included to analyze the key proteins and predict the key bioactive compounds. The results indicated that peiminine (PEI) target epidermal growth factor receptor (EGFR) prevented the exacerbation of COPD by inhibiting the EGFR signaling pathway, and ursolic acid (UA) can alleviate inflammation disorders via inhibition of CASP3 on mitogen-activated protein kinase (MAPK) signaling pathway. After in vivo and in vitro evaluations, we revealed that PEI from CBPP, as a lead compound, can improve lung function and alleviate pulmonary fibrosis by acting on the EGFR and MLC2 signaling pathways. Furthermore, the approach described here is an effective way to analyze and identify the bioactive ingredients from a mixture by functional proteomics analysis.

19.
Gastroenterology ; 157(5): 1413-1428.e11, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31352001

RESUMO

BACKGROUND & AIMS: Obesity is a risk factor for pancreatic cancer. In mice, a high-fat diet (HFD) and expression of oncogenic KRAS lead to development of invasive pancreatic ductal adenocarcinoma (PDAC) by unknown mechanisms. We investigated how oncogenic KRAS regulates the expression of fibroblast growth factor 21, FGF21, a metabolic regulator that prevents obesity, and the effects of recombinant human FGF21 (rhFGF21) on pancreatic tumorigenesis. METHODS: We performed immunohistochemical analyses of FGF21 levels in human pancreatic tissue arrays, comprising 59 PDAC specimens and 45 nontumor tissues. We also studied mice with tamoxifen-inducible expression of oncogenic KRAS in acinar cells (KrasG12D/+ mice) and fElasCreERT mice (controls). KrasG12D/+ mice were placed on an HFD or regular chow diet (control) and given injections of rhFGF21 or vehicle; pancreata were collected and analyzed by histology, immunoblots, quantitative polymerase chain reaction, and immunohistochemistry. We measured markers of inflammation in the pancreas, liver, and adipose tissue. Activity of RAS was measured based on the amount of bound guanosine triphosphate. RESULTS: Pancreatic tissues of mice expressed high levels of FGF21 compared with liver tissues. FGF21 and its receptor proteins were expressed by acinar cells. Acinar cells that expressed KrasG12D/+ had significantly lower expression of Fgf21 messenger RNA compared with acinar cells from control mice, partly due to down-regulation of PPARG expression-a transcription factor that activates Fgf21 transcription. Pancreata from KrasG12D/+ mice on a control diet and given injections of rhFGF21 had reduced pancreatic inflammation, infiltration by immune cells, and acinar-to-ductal metaplasia compared with mice given injections of vehicle. HFD-fed KrasG12D/+ mice given injections of vehicle accumulated abdominal fat, developed extensive inflammation, pancreatic cysts, and high-grade pancreatic intraepithelial neoplasias (PanINs); half the mice developed PDAC with liver metastases. HFD-fed KrasG12D/+ mice given injections of rhFGF21 had reduced accumulation of abdominal fat and pancreatic triglycerides, fewer pancreatic cysts, reduced systemic and pancreatic markers of inflammation, fewer PanINs, and longer survival-only approximately 12% of the mice developed PDACs, and none of the mice had metastases. Pancreata from HFD-fed KrasG12D/+ mice given injections of rhFGF21 had lower levels of active RAS than from mice given vehicle. CONCLUSIONS: Normal acinar cells from mice and humans express high levels of FGF21. In mice, acinar expression of oncogenic KRAS significantly reduces FGF21 expression. When these mice are placed on an HFD, they develop extensive inflammation, pancreatic cysts, PanINs, and PDACs, which are reduced by injection of FGF21. FGF21 also reduces the guanosine triphosphate binding capacity of RAS. FGF21 might be used in the prevention or treatment of pancreatic cancer.


Assuntos
Células Acinares/metabolismo , Carcinoma Ductal Pancreático/metabolismo , Transformação Celular Neoplásica/metabolismo , Dieta Hiperlipídica , Fatores de Crescimento de Fibroblastos/metabolismo , Neoplasias Intraductais Pancreáticas/metabolismo , Neoplasias Pancreáticas/metabolismo , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Células Acinares/patologia , Animais , Carcinoma Ductal Pancreático/genética , Carcinoma Ductal Pancreático/patologia , Carcinoma Ductal Pancreático/prevenção & controle , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/patologia , Regulação para Baixo , Fatores de Crescimento de Fibroblastos/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos Transgênicos , Mutação , PPAR gama/genética , PPAR gama/metabolismo , Cisto Pancreático/genética , Cisto Pancreático/metabolismo , Cisto Pancreático/patologia , Neoplasias Intraductais Pancreáticas/genética , Neoplasias Intraductais Pancreáticas/patologia , Neoplasias Intraductais Pancreáticas/prevenção & controle , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/patologia , Neoplasias Pancreáticas/prevenção & controle , Pancreatite/genética , Pancreatite/metabolismo , Pancreatite/patologia , Proteínas Proto-Oncogênicas p21(ras)/genética , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/genética , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/metabolismo , Transdução de Sinais , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
20.
Artigo em Inglês | MEDLINE | ID: mdl-31294676

RESUMO

The purpose of this study was to investigate pesticide residues in bell peppers from Shandong Province, China. A total of 299 samples were collected from 17 cities in 2016. The concentrations of 26 pesticide residues were determined by gas chromatography with mass spectrometry (GC-MS). The results showed that there were 25 pesticides (15 OPs, 7 PYs, 3 CBs) found in 86 bell pepper samples, and the total number of positives was 120. The total frequency was 28.76%. The detection frequency for OPs, PYs and CBs was 16.39%, 12.37% and 3.01%, respectively. The most frequently detected pesticide was bifenthrin, with the frequency of 5.02%. 5.35% of samples contained pesticide residues above the maximum residue limits (MRLs) set by China. 7.36% of samples contained more than one pesticide. The values of %ADI were below 100, while the %ARfD of carbofuran and methidathion exceeded 100 for children. The cumulative risk was highest for OPs. From the public health point of view, the levels of pesticide residues in bell peppers do not pose a serious health risk to adults, but the acute health risk to children should be paid more attention.


Assuntos
Capsicum/química , Contaminação de Alimentos/análise , Resíduos de Praguicidas/análise , Piretrinas/análise , Adulto , Criança , China , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Controle de Qualidade , Medição de Risco , Extração em Fase Sólida
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA