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1.
J Sci Food Agric ; 103(1): 380-388, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35894931

RESUMO

BACKGROUND: Studies have found that the addition of plant essential oils to feed had a positive effect on intestinal microflora and immunity in mice. However, the effect of different ways of ingestion of orange essential oil on mice has seldom been reported. In the present study, we investigated the effects of ingestion of orange essential oil by gavage, sniffing and feeding on intestinal microflora and immunity in mice. RESULTS: The results obtained showed that a low concentration of essential oil feeding significantly increased the spleen index of mice (P < 0.05). The effect of different ways of ingestion on the thymus index, immunoglobulin G and immunoglobulin M of mice was not significant (P > 0.05). High and medium concentrations of essential oil feeding increased the level of interleukin-2 in mice (P < 0.05). H+ K+ -ATPase activity was significantly increased in mice fed with gavage and different concentrations of essential oil feed compared to the control group (P < 0.05). The analysis of the results of the microflora in the cecum and colon of mice indicated that the medium concentration of essential oil feeding group and the sniffing group significantly changed the structure of the flora and increased the diversity of the intestinal microflora. All three essential oil ingestion methods increased the abundance of Bacteroidetes and Lactobacillus in the intestine of mice. CONCLUSION: Compared with gavage and feeding, sniffing had a significant effect on immunoglobulins in mice. All the three ingestion methods could affect the intestinal microflora of mice and increase the abundance of Lactobacillus. © 2022 Society of Chemical Industry.


Assuntos
Microbioma Gastrointestinal , Óleos Voláteis , Óleos Voláteis/farmacologia , Lactobacillus , Intestinos , Ceco
2.
J Colloid Interface Sci ; 629(Pt A): 388-398, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36087554

RESUMO

As a promising high energy density cathode, single-crystal Ni-rich cathode face poor diffusion dynamics, which leads to poor structural evolution, poor cyclic stability and unfavorable rate performance, thus impeding its wider application. Herein, the strategy of synergistic surface modification by ionic conductor coating and trace element doping is delicately designed. The surface protective Li3BO3 layer is wrapped on the single-crystal LiNi0.83Co0.11Mn0.06O2 (NCM83), which can improve the compatibility of cathode/electrolyte with reduced interface resistance. While Zr is incorporated into bulk to stabilize the crystal structure and migration channel. This synergistic strategy achieves the improvement of ionic transport and structural stability of single-crystal NCM83 (Zr-NCM83@B) from the outer surface to the inner body. As expected, the modified cathode Zr-NCM83@B demonstrates a satisfying electrochemical performance. It delivers a high reversible capacity of 169 mAh g-1 in coin-type half-cell at 4C within 3.0-4.3 V. Remarkably, it displays excellent capacity retention of 83.5 % in Zr-NCM83@B || graphite pouch-type full-cell over 1400 cycles at 1C with high voltage range of 2.8-4.4 V. This synergistic surface modification provides a reference for commercial development of advanced single-crystal Ni-rich cathode under harsh testing conditions.

3.
Sci Total Environ ; 856(Pt 1): 159071, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36179833

RESUMO

Microplastic accumulation in the lake aquatic environment has attracted worldwide attention, but the long-term deposition characteristics and influencing factors of microplastics are not clear. Hence, we attempted to analyze the factors that influence the accumulation of microplastics during the process of natural environmental change and social development through the method of the sedimentological approach. In this investigation, the chronological sequence of sediment core was set up based on lead-210 composition to study microplastic accumulation rates in a plateau lake, Jianhu Lake. For the first time, the records of microplastic deposition were analyzed combined with multiple anthropogenic and natural factors, including 4 natural and 17 social factors to reveal the influence of natural processes and social development on microplastic abundances. The results showed that sediments were highly contaminated by microplastics, and the mean abundance of microplastics in the sediment cores of Jianhu Lake was 924 ± 427 particles/kg, and showed a gradually increasing trend from the bottom (25 to 30 cm) to surface layer (0 to 5 cm). Blue and black were the main colors of microplastics, and the average proportion of wirelike microplastics was 63 %. Additionally, the polymer type of microplastic was mainly rayon, the surface morphology of different polymer types was disparate, and various elements enriched on the surface of microplastics were also detected. In the past 70 years, the deposition rate of microplastics in Jianhu Lake maintained the trend of continuous growth, and the abundance of microplastics is projected to be 448 to 3017 and 513 to 3670 particles/kg by 2035 and 2050, respectively. What is more, there are significant correlations among multiple natural and anthropogenic factors and microplastic deposition, as well as microplastic polymer species.


Assuntos
Microplásticos , Poluentes Químicos da Água , Lagos , Plásticos , Sedimentos Geológicos , Efeitos Antropogênicos , Monitoramento Ambiental , Poluentes Químicos da Água/análise
4.
J Environ Manage ; 325(Pt B): 116536, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36326523

RESUMO

Cleaner winter heating has been promoted to abate the winter air pollution in northern China. Although improvements in air quality have been observed, the effectiveness and mechanism of cleaner heating measures on air quality have not been examined on the empirical ground. In this study, we estimate the annual effects of winter heating policy on air quality from 2014 to 2017 using a regression discontinuity design (RDD) and dynamic regression model. The results show that winter heating aggravates Air Quality Index (AQI). Specifically, the AQI raised by winter heating reduce from 85.3 in 2014 to 24.1 in 2017, indicating diminishing effects of winter heating with the implementation of clean heating measures. The heterogeneous characteristics of winter heating in terms of different pollutants and city scales are further quantified. The effects of clean heating are more evident for particulate pollutants (PM2.5 and PM10) than for SO2, NO2, CO and O3. The promotion of clean heating is more effective in larger cities. These findings provided insights into the diminishing air pollution change with continuous advancement in clean heating policy and the heterogeneity among cities and pollutants should be taken into account when formulating future policies in response to energy transition and climate change.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Material Particulado/análise , Calefação , Poluição do Ar/prevenção & controle , Poluição do Ar/análise , Cidades , China , Monitoramento Ambiental/métodos
5.
Neural Regen Res ; 18(1): 141-149, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35799534

RESUMO

Neuroinflammation and the NACHT, LRR, and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury (TBI). Maraviroc, a C-C chemokine receptor type 5 antagonist, has been viewed as a new therapeutic strategy for many neuroinflammatory diseases. We studied the effect of maraviroc on TBI-induced neuroinflammation. A moderate-TBI mouse model was subjected to a controlled cortical impact device. Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days. Western blot, immunohistochemistry, and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI. Our results suggest that maraviroc administration reduced NACHT, LRR, and PYD domains-containing protein 3 inflammasome activation, modulated microglial polarization from M1 to M2, decreased neutrophil and macrophage infiltration, and inhibited the release of inflammatory factors after TBI. Moreover, maraviroc treatment decreased the activation of neurotoxic reactive astrocytes, which, in turn, exacerbated neuronal cell death. Additionally, we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score, rotarod test, Morris water maze test, and lesion volume measurements. In summary, our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI, and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.

6.
Neural Regen Res ; 18(4): 708-715, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36204826

RESUMO

Alzheimer's disease is characterized by sustained neuroinflammation leading to memory loss and cognitive decline. The past decade has witnessed tremendous efforts in Alzheimer's disease research; however, no effective treatment is available to prevent disease progression. An increasing body of evidence suggests that neuroinflammation plays an important role in Alzheimer's disease pathogenesis, alongside the classical pathological hallmarks such as misfolded and aggregated proteins (e.g., amyloid-beta and tau). Firstly, this review summarized the clinical and pathological characteristics of Alzheimer's disease. Secondly, we outlined key aspects of glial cell-associated inflammation in Alzheimer's disease pathogenesis and provided the latest evidence on the roles of microglia and astrocytes in Alzheimer's disease pathology. Then, we revealed the double-edged nature of inflammatory cytokines and inflammasomes in Alzheimer's disease. In addition, the potential therapeutic roles of innate immunity and neuroinflammation for Alzheimer's disease were also discussed through these mechanisms. In the final section, the remaining key problems according to the current research status were discussed.

7.
Food Chem ; 404(Pt A): 134526, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36265276

RESUMO

Nanoliposome encapsulation combined with carboxymethyl chitosan (CMCS) surface decoration was employed to improve physicochemical stability and oral bioavailability of alpha-linolenic acid (ALA). Different nanoliposome systems including ALA-loaded nanoliposomes (ALA-NLs) and CMCS-coated ALA-NLs (CMCS-ALA-NLs) were characterized through dynamic light scattering, transmission electron microscope, Fourier transform infrared spectroscopy and differential scanning calorimetry. The results showed that CMCS-ALA-NLs had good encapsulation efficiency of 79% and layer formation with nanosized spherical carrier. The physicochemical stability of CMCS-ALA-NLs was better than that of ALA-NLs. CMCS-ALA-NLs were able to regulate the release of ALA in a simulated gastrointestinal environment. In vivo testing found that ALA concentration of CMCS-ALA-NLs had an area under the curve of 1.32, which was 1.28 times higher than that of ALA-NLs and 2 times higher than that of ALA-emulsion. The absorption of ALA was improved by CMCS-ALA-NLs. It suggested that CMCS-coated nanoliposomes should be an available delivery strategy for transporting ALA.

8.
Sci Total Environ ; 857(Pt 2): 159452, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36265630

RESUMO

The present study investigated the temporal and spatial distributions, partition behaviors, sources, and risks of 14 perfluoroalkyl acids (PFAAs) in the aquatic environment of a fluorine industry-impacted region. The total concentrations of 14 PFAAs (ΣPFAAs) were 118.10-2235.4 ng/L, 40.00-2316.1 ng/g dw, and 6.90-180.5 ng/g dw in dissolved, suspended particle matter (SPM), and sedimentary phases, respectively. The predominant pollutants in the dissolved and SPM phases were perfluoroalkyl carboxylic acids (PFCAs) with carbon chain lengths <9, whereas C13 and C14 PFCAs accounted for a large proportion in the sedimentary phase. The dry season exhibited the highest concentration of ΣPFAAs in the dissolved phase (500.9 ± 350.2 ng/L), while the wet season showed the highest concentrations of ΣPFAAs in the SPM and sedimentary phases (591.6 ± 469.1 ng/g dw and 59.7 ± 35.5 ng/g dw, respectively). Significantly higher concentrations of PFAAs have been found in sewage plant and industrial areas. The concentration of PFAAs in the Xupu water source area (XPS) was slightly higher than that in other water source areas of the Yangtze River, which were either not affected or were less affected by the fluorine industry. The log KD-SPM (distribution coefficient between SPM and water), log KD-SED (distribution coefficient between sediment and water), and log KOC-SED (the organic carbon normalized distribution coefficient) of PFAAs showed significant differences between the wet season and dry season, which may also be affected by carbon chain length. Source identification results showed that industries, wastewater discharge, and nonpoint sources were the main sources of PFAAs in this region. The ecological risk posed by long-chain PFAAs in aquatic organisms cannot be ignored, especially in areas with intensive industrial and agricultural activities. Health risks may exist for local toddlers with long-term exposure to perfluorooctanoic acid (PFOA) through drinking water intake and dermal contact.


Assuntos
Ácidos Alcanossulfônicos , Fluorcarbonetos , Poluentes Químicos da Água , Fluorcarbonetos/análise , Flúor , Monitoramento Ambiental/métodos , Poluentes Químicos da Água/análise , Rios , Medição de Risco , Ácidos Carboxílicos , Água , Carbono , Ácidos Alcanossulfônicos/análise , China
9.
Environ Toxicol ; 2022 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-36336961

RESUMO

Laryngeal cancer (LC) is the most common aggressive malignancy of the head and neck. LncRNA ZNFX1 antisense RNA 1 (ZFAS1) displays oncogenic properties in head and neck squamous cell carcinoma, but its regulatory role in laryngeal cancer progression remains obscure. Here, we found that ZFAS1 expression in laryngeal cancer tissues and cells was higher than that in adjacent normal tissues and normal nasopharyngeal epithelial cells. Highly expressed ZFAS1 was associated with advanced lymph node metastasis stages and clinical stages. ZFAS1 overexpression promoted LC cell proliferation, invasion, and N-cadherin and Vimentin expression, and suppressed E-cadherin expression. While ZFAS1 knockdown played an opposite role. Mechanistically, ZFAS1 stabilized RNA binding fox-1 homolog 2 (RBFOX2) protein expression by binding to RBFOX2, and RBFOX2 overexpression reversed the effect of ZFAS1 silence on cell functions. Moreover, highly expressed RBFOX2 led to skipping of MENA exon 11a and generating a pro-invasive isoform (MENAINV ). MENAINV overexpression effectively abolished the inhibitory effect of RBFOX2 knockdown on cell malignant progression. Furthermore, Hep2 cells infected with lentivirus-mediated ZFAS1 shRNA or negative control shRNA were subcutaneously injected into mice to assess the role of ZFAS1 in tumor growth. And the data showed that silencing ZFAS1 in vivo hindered xenograft tumor growth. In conclusion, silencing ZFAS1 alleviated malignant progression of laryngeal cancer cells and mouse xenograft tumor growth by regulating RBFOX2-mediated alternative splicing of MENA.

10.
Mol Cell Biol ; : e0028922, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36342127

RESUMO

PURPL is a p53-induced lncRNA that suppresses basal p53 levels. Here, we investigated PURPL upon p53 activation in liver cancer cells, where it is expressed at significantly higher levels than other cell types. Using isoform sequencing, we discovered novel PURPL transcripts that have a retained intron and/or previously unannotated exons. To determine PURPL function upon p53 activation, we performed transcriptome sequencing (RNA-Seq) after depleting PURPL using CRISPR interference (CRISPRi), followed by Nutlin treatment to induce p53. Strikingly, although loss of PURPL in untreated cells altered the expression of only 7 genes, loss of PURPL resulted in altered expression of ~800 genes upon p53 activation, revealing a context-dependent function of PURPL. Pathway analysis suggested that PURPL is important for fine-tuning the expression of specific genes required for mitosis. Consistent with these results, we observed a significant decrease in the percentage of mitotic cells upon PURPL depletion. Collectively, these data identify novel transcripts from the PURPL locus and suggest that PURPL delicately moderates the expression of mitotic genes in the context of p53 activation to control cell cycle arrest.

11.
Dalton Trans ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36342404

RESUMO

The subcomponent self-assembly of 4,6-dihydrazinopyrimidine and o-vanillin/salicylaldehyde with different DyIII salts leads to the formation of Dy2 (1-3) and grid-like Dy4 (4) compounds with formulas of [Dy2L(DMF)4(NO3)4]·2DMF (1), [Dy2L2(CH3COO)4(CH3OH)2]·10H2O (2), [Dy2L'2(CH3COO)4(CH3OH)2]·2H2O·2CH3OH (3) and [Dy4Na2L2(µ2-OH)2(PhCOO)8(CH3OH)(H2O)]·CH3CN·2CH3OH·7H2O (4). In all cases, the DyIII ions are nine coordinate but the different features of coordinated solvent molecules and anions result in diverse coordination geometries in their respective structures. Notably, by introducing various axial coordinating anions with different electronegativities (NO3-, CH3COO- and C6H5COO-), the effective energy barriers were progressively enhanced (52-207 K) in this system. Detailed magnetic studies for these complexes revealed obvious magnetic interactions in complex 1 and clear single molecule magnet (SMM) behavior with anisotropy barriers of 52, 56 and 58.4 K for 1-3, respectively. Moreover, we find that the incorporation of benzoates into the axial position of the DyIII ions in complex 4 leads to the occurrence of two clear distinct relaxation processes, with energy barriers of 94.9 and 207.2 K, respectively. This result presents an example of the effects of axial coordinating anions on the SMM behavior, providing a valid route towards enhancing the slow magnetic relaxation of 4f based SMMs via incorporating diverse coordination anions into the subcomponent self-assembly system.

12.
Chin J Integr Med ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36342592

RESUMO

Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) have recently been identified to be closely related to the occurrence and development of atherosclerosis (AS). A growing body of evidence has suggested Chinese medicine takes unique advantages in preventing and treating AS. In this review, the related research progress of AS and LOX-1 has been summarized. And the anti-AS effects of 10 active components of herbal medicine through LOX-1 regulation have been further reviewed. As a potential biomarker and target for intervention in AS, LOX-1 targeted therapy might provide a promising and novel approach to atherosclerotic prevention and treatment.

13.
BMC Cardiovasc Disord ; 22(1): 458, 2022 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-36324069

RESUMO

BACKGROUND: Bilirubin is a heme catabolism product with antioxidant, anti-inflammatory, and anti-apoptotic properties and is implicated in the prognosis of several diseases. This study evaluates the prognostic role of bilirubin in coronary artery disease (CAD) patients. METHODS: After identifying studies from the literature, meta-analyses were performed to achieve a) overall estimates of serum total bilirubin levels in patients with myocardial infarction (MI), non-MI CAD and healthy individuals; b) odds ratios (OR) of adverse outcomes between higher and lower total bilirubin levels; c) standardized mean difference (SMD) in total bilirubin levels in patients with high vs low CAD severity; and d) correlation between disease severity and total bilirubin. Metaregression analyses were performed to examine the relationship between cardiovascular risk factors and increasing quantiles of total bilirubin levels. RESULTS: Forty-three studies were identified. Pooled serum total bilirubin levels were 0.72 mg/dl [95% confidence interval (CI): 0.60, 0.83] in MI patients; 0.65 mg/dl [95% CI: 0.60, 0.69] in non-MI CAD patients; and 0.66 mg/dl [95% CI: 0.56, 0.75] in healthy individuals. Higher total bilirubin levels were associated with greater odds of adverse outcomes in MI patients (OR: 1.08 [95% CI: 0.99, 1.18]) but lower odds in non-MI CAD patients (OR: 0.80 [95%CI: 0.73, 0.88]). Compared to non-severe cases, total bilirubin levels were higher in patients with severe MI (SMD 0.96 [95% CI: - 0.10, 2.01]; p = 0.074) but were lower in severe non-MI CAD patients (SMD - 0.30 [95%CI: - 0.56, - 0.03]; p = 0.02). Total bilirubin levels correlated positively with MI severity (r = 0.41 [95% CI: 0.24, 0.59]; p < 0.01) but correlated negatively with non-MI CAD severity (r = - 0.17 [95% CI: - 0.48, 0.14]; p = 0.28). Female sex was inversely associated with increasing quantiles of bilirubin (meta-regression coefficient: - 8.164 [- 14.531, - 1.769]; p = 0.016) in MI patients. CONCLUSION: Prognostic role of bilirubin for CAD appears complicated, as different odds are observed for MI and non-MI CAD patients which weakens the case of causal involvement of bilirubin in CAD etiology or prognosis.


Assuntos
Doença da Artéria Coronariana , Infarto do Miocárdio , Humanos , Feminino , Doença da Artéria Coronariana/diagnóstico , Bilirrubina , Prognóstico , Razão de Chances , Fatores de Risco
14.
BMC Cancer ; 22(1): 1129, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329397

RESUMO

PURPOSE: To analyze the clinical outcomes of patients with regional persistent/recurrent nasopharyngeal carcinoma (NPC) who received neck dissection, and to evaluate the clinical benefit of postoperative adjuvant therapy (PAT) based on patients' positive lymph node counts (PLNs), extracapsular spread (ECS) and preoperative plasma EBV DNA levels. METHODS: From 2003 to 2017, 342 patients with regional persistent/recurrent NPC were included in this study. All patients were treated with neck dissection and 76 patients received PAT. Progression-free survival (PFS), overall survival (OS), distant metastasis-free survival (DMFS) and locoregional relapse-free survival (LRFS) were compared between groups using propensity score matching (PSM). RESULTS: 152 patients without PAT treatment and 76 patients with PAT treatment were selected by the PSM. There was no significant difference in 2-year PFS (52.4% vs. 61.3%, P = 0.371), 2-year OS (91.9% vs. 90.5%, P = 0.097) or 2-year LRFS (66.3% vs. 67.9%, P = 0.872) between the two groups. However, the application of PAT brought survival benefits to patients in terms of 2-year DMFS (76.5% vs. 84.7%, P = 0.020). PLN, ECS and preoperative EBV DNA level remained independent risk factors for poorer PFS. Accordingly, patients were divided into low-risk and high-risk groups using receiver operating characteristic (ROC) curve; the 2-year PFS rates for two risk groups were 73.4% and 59.1% (P < 0.0001) respectively. The results showed that low-risk patients didn't benefit from the addition of PAT. However, the 2-year DMFS rate was significantly improved in high-risk PAT-treated patients than those treated by neck dissection alone (83.7% vs. 71.7%, P = 0.023). CONCLUSIONS: PLNs, ECS and preoperative EBV DNA level are associated with the prognosis of patients with regional persistent/recurrent NPC. High-risk patients identified by PLNs, ECS and preoperative EBV DNA level may benefit from the addition of PAT after neck dissection.


Assuntos
Infecções por Vírus Epstein-Barr , Neoplasias Nasofaríngeas , Humanos , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/cirurgia , Esvaziamento Cervical , Herpesvirus Humano 4/genética , DNA Viral , Recidiva Local de Neoplasia , Prognóstico , Estudos Retrospectivos
15.
Cancer Imaging ; 22(1): 62, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333763

RESUMO

BACKGROUND: Esophageal fistula is one of the most serious complications of chemotherapy or chemoradiotherapy (CRT) for advanced esophageal cancer. This study aimed to evaluate the performance of quantitative computed tomography (CT) analysis and to establish a practical imaging model for predicting esophageal fistula in esophageal cancer patients treated with chemotherapy or chemoradiotherapy. METHODS: This study retrospectively enrolled 204 esophageal cancer patients (54 patients with fistula, 150 patients without fistula) and all patients were allocated to the primary and validation cohorts according to the time of inclusion in a 1:1 ratio. Ulcer depth, tumor thickness and length, and minimum and maximum enhanced CT values of esophageal cancer were measured in pretreatment CT imaging. Logistic regression analysis was used to evaluate the associations of CT quantitative measurements with esophageal fistula. Receiver operating characteristic curve (ROC) analysis was also used. RESULTS: Logistic regression analysis showed that independent predictors of esophageal fistula included tumor thickness [odds ratio (OR) = 1.167; p = 0.037], the ratio of ulcer depth to adjacent tumor thickness (OR = 164.947; p < 0.001), and the ratio of minimum to maximum enhanced CT value (OR = 0.006; p = 0.039) in the primary cohort at baseline CT imaging. These predictors were used to establish a predictive model for predicting esophageal fistula, with areas under the receiver operating characteristic curves (AUCs) of 0.946 and 0.841 in the primary and validation cohorts, respectively. The quantitative analysis combined with T stage for predicting esophageal fistula had AUCs of 0.953 and 0.917 in primary and validation cohorts, respectively. CONCLUSION: Quantitative pretreatment CT analysis has excellent performance for predicting fistula formation in esophageal cancer patients who treated by chemotherapy or chemoradiotherapy.


Assuntos
Fístula Esofágica , Neoplasias Esofágicas , Humanos , Estudos Retrospectivos , Úlcera , Quimiorradioterapia/efeitos adversos , Neoplasias Esofágicas/terapia , Neoplasias Esofágicas/patologia , Tomografia Computadorizada por Raios X , Fístula Esofágica/diagnóstico por imagem , Fístula Esofágica/etiologia , Fluordesoxiglucose F18
16.
Nat Commun ; 13(1): 6689, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36335125

RESUMO

Recurrence is frequent in pediatric ependymoma (EPN). Our longitudinal integrated analysis of 30 patient-matched repeated relapses (3.67 ± 1.76 times) over 13 years (5.8 ± 3.8) reveals stable molecular subtypes (RELA and PFA) and convergent DNA methylation reprogramming during serial relapses accompanied by increased orthotopic patient derived xenograft (PDX) (13/27) formation in the late recurrences. A set of differentially methylated CpGs (DMCs) and DNA methylation regions (DMRs) are found to persist in primary and relapse tumors (potential driver DMCs) and are acquired exclusively in the relapses (potential booster DMCs). Integrating with RNAseq reveals differentially expressed genes regulated by potential driver DMRs (CACNA1H, SLC12A7, RARA in RELA and HSPB8, GMPR, ITGB4 in PFA) and potential booster DMRs (PLEKHG1 in RELA and NOTCH, EPHA2, SUFU, FOXJ1 in PFA tumors). DMCs predicators of relapse are also identified in the primary tumors. This study provides a high-resolution epigenetic roadmap of serial EPN relapses and 13 orthotopic PDX models to facilitate biological and preclinical studies.


Assuntos
Ependimoma , Simportadores , Humanos , Criança , Ependimoma/genética , Ependimoma/patologia , Metilação de DNA/genética , Recidiva , Epigênese Genética , Simportadores/genética
17.
Front Biosci (Landmark Ed) ; 27(10): 295, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36336853

RESUMO

BACKGROUND: Recently, single-cell RNA sequencing (scRNA-seq) technology was increasingly used to study transcriptomics at a single-cell resolution, scRNA-seq analysis was complicated by the "dropout", where the data only captures a small fraction of the transcriptome. This phenomenon can lead to the fact that the actual expressed transcript may not be detected. We previously performed osteoblast subtypes classification and dissection on freshly isolated human osteoblasts. MATERIALS AND METHODS: Here, we used the scImpute method to impute the missing values of dropout genes from a scRNA-seq dataset generated on freshly isolated human osteoblasts. RESULTS: Based on the imputed gene expression patterns, we discovered three new osteoblast subtypes. Specifically, these newfound osteoblast subtypes are osteoblast progenitors, and two undetermined osteoblasts. Osteoblast progenitors showed significantly high expression of proliferation related genes (FOS, JUN, JUNB and JUND). Analysis of each subtype showed that in addition to bone formation, these undetermined osteoblasts may involve osteoclast and adipocyte differentiation and have the potential function of regulate immune activation. CONCLUSIONS: Our findings provided a new perspective for studying the osteoblast heterogeneity and potential biological functions of these freshly isolated human osteoblasts at the single-cell level, which provides further insight into osteoblasts subtypes under various (pathological) physiological conditions.


Assuntos
Osteoblastos , Transcriptoma , Humanos , RNA-Seq , Osteoblastos/metabolismo , Diferenciação Celular/genética , Osteogênese/genética , Perfilação da Expressão Gênica
18.
Polymers (Basel) ; 14(21)2022 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-36365464

RESUMO

In order to investigate the effectiveness of polymer modification and fiber reinforcement on the cracking and impact resistance of concrete materials prepared for ultra-thin whitetopping (UTW), carboxyl butyl benzene latex and polyformaldehyde fibers were added to the conventional cement concrete mix. In addition, test methods that used an asphalt mixture performance tester (AMPT) and mechanical rammer were developed to evaluate concrete cracking and impact resistance, respectively. Results from the AMPT test revealed that the cracking resistance can be enhanced with polymer and fiber, especially the initial tensile load peak, which can be improved by more than 40% when fiber and polymer compound modification is applied. Meanwhile, the impact loading test revealed that the inclusion of both fiber and polymer results in a two-fold increase in the number of impacts before visible cracking occurs, and the number of blows to failure increased by 21.4%. Moreover, microstructures were investigated by scanning electron microscopy (SEM) to confirm the reinforcing mechanism of both polymer modification and fiber reinforcement.

19.
J Int Med Res ; 50(11): 3000605221135864, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36366735

RESUMO

OBJECTIVE: High-grade serous ovarian cancer (HGSOC) is a deadly malignancy. Homeobox protein A9 (HOXA9) is linked with serous papillary histotype differentiation, and inappropriate HOXA9 expression is a step in ovarian cancer that induces aberrant differentiation. This study aimed to reveal the significance of HOXA9 in HGSOC. METHODS: HOXA9 mRNA and protein expression were examined by quantitative PCR and immunohistochemistry, respectively. The chi-square test was used to evaluate associations between HOXA9 expression and clinical characteristics. The prognostic value of HOXA9 was calculated by the Kaplan-Meier method. The Kaplan-Meier Plotter database was used to assess the prognostic value of HOXA9. RESULTS: The mRNA and protein expression of HOXA9 were significantly upregulated in chemotherapy-resistant HGSOC compared with chemotherapy-sensitive HGSOC. The chi-square test showed that high HOXA9 expression was significantly related with grade, clinical stage, and residual disease. High HOXA9 expression was significantly associated with poor prognosis. The Kaplan-Meier Plotter database further confirmed these results. Cox hazard regression showed that high HOXA9 expression was an independent prognostic factor for survival in HGSOC patients. CONCLUSION: This study showed that HOXA9 expression was associated with chemotherapy resistance and poor outcomes in HGSOC patients. High HOXA9 expression might be a prognostic indicator for HGSOC.


Assuntos
Cistadenocarcinoma Seroso , Neoplasias Ovarianas , Humanos , Feminino , Cistadenocarcinoma Seroso/tratamento farmacológico , Cistadenocarcinoma Seroso/genética , Cistadenocarcinoma Seroso/patologia , Estimativa de Kaplan-Meier , Prognóstico , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/patologia , Expressão Gênica , RNA Mensageiro/genética
20.
Chem Asian J ; 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36411242

RESUMO

As a critical action plan formulated for peaking carbon dioxide emissions, polymeric electromagnetic shielding materials (EMI) based on CO2 foaming technology have recently been attracting widespread attention in both research and industry, attributable to their efficient use of CO2, high specific strength, corrosion resistance and low-cost characteristics. In the past decade, the emergence of novel design concepts and preparation techniques for CO2 foaming technology has led to the development of new high-performance electromagnetic shielding materials in this field. This review summarizes the research progress made to date on the fabrication of EMI shielding composite foams by supercritical carbon dioxide (scCO2) foaming. We also explore the structure-activity relationships between the component/distribution and EMI shielding properties. Additionally, the application prospects and development challenges of new EMI shielding composite foams are expounded.

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