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1.
HLA ; 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33258295

RESUMO

HLA-B*55:71 has one nucleotide change from HLA-B*55:02:01:01 where Histidine (3) is changed to Tyrosine. This article is protected by copyright. All rights reserved.

2.
Cell Rep ; 33(5): 108342, 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33147462

RESUMO

Influenza A virus (IAV) infection stimulates a type I interferon (IFN-I) response in host cells that exerts antiviral effects by inducing the expression of hundreds of IFN-stimulated genes (ISGs). However, most ISGs are poorly studied for their roles in the infection of IAV. Herein, we demonstrate that SERTA domain containing 3 (SERTAD3) has a significant inhibitory effect on IAV replication in vitro. More importantly, Sertad3-/- mice develop more severe symptoms upon IAV infection. Mechanistically, we find SERTAD3 reduces IAV replication through interacting with viral polymerase basic protein 2 (PB2), polymerase basic protein 1 (PB1), and polymerase acidic protein (PA) to disrupt the formation of the RNA-dependent RNA polymerase (RdRp) complex. We further identify an 8-amino-acid peptide of SERTAD3 as a minimum interacting motif that can disrupt RdRp complex formation and inhibit IAV replication. Thus, our studies not only identify SERTAD3 as an antiviral ISG, but also provide the mechanism of potential application of SERTAD3-derived peptide in suppressing influenza replication.

3.
Eur Radiol ; 2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33201286

RESUMO

OBJECTIVE: The purpose of this study was to develop a classification method based on support vector machine (SVM) to improve the diagnostic performance of 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography (PET/CT) to detect the lymph node (LN) metastasis in non-small cell lung cancer (NSCLC). METHOD: Two hundred nineteen lymph nodes (37 metastatic) from 71 patients were evaluated in this study. SVM models were developed with 7 LN features. The area under the curve (AUC) and accuracy of 9 models were compared to select the best model. The best SVM model was simplified on the basis of the feature weights and value distribution to further suit the clinical application. RESULTS: The maximum, minimum, and mean accuracy of the best model was 91.89% (68/74, 95% CI 83.11~96.54%), 66.22% (49/74, 95% CI 54.85~75.98%), and 80.09% (59,266/74,000, 95% CI 70.27~89.19%), respectively, with an AUC of 0.94, 0.66, and 0.81, respectively. The best SVM model was finally simplified into a score rule: LNs with scores more than 3.0 were considered as malignant ones, whereas LNs with scores less than 1.5 tended to be benign ones. For the LNs with scores within a range of 1.5-3.0, metastasis was suspected. CONCLUSION: An SVM model based on 18F-FDG PET/CT images was able to predict the metastatic LNs for patients with NSCLC. The ratio of the maximum of standard uptake value of LNs to aortic arch played a major role in the model. After simplification, the model could be transferred into a scoring method which may partly help clinicians determine the clinical staging of patients with NSCLC relatively easier. KEY POINTS: • The SVM model based on 18F-FDG PET/CT features may help clinicians to make a decision for metastatic mediastinal lymph nodes in patients with NSCLC. • The SURblood plays a major role in the SVM model. • The score rule based on the SVM model simplified the complexity of the model and may partly help clinicians determine the clinical staging of patients with NSCLC relatively easier.

4.
Life Sci ; : 118806, 2020 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-33249098

RESUMO

AIMS: Neuronal apoptosis acts as the pivotal pathogenesis of cerebral ischemia/reperfusion (I/R) injury after ischemic stroke. PAQR3 (progestin and adipoQ receptor family member 3) is a crucial player who participates in the regulation of cell death. We aim to explore the specific function and the underlying mechanism of PAQR3 in cerebral I/R induced neuronal injury. MAIN METHODS: We established a mouse middle cerebral artery occlusion/reperfusion (MCAO/R) model and rat adrenal pheochromocytoma (PC12) cell oxygen-glucose deprivation/reperfusion (OGD/R) model to detect the expression and of PAQR3 after I/R treatment in vivo and in vitro. We used lentivirus to knockdown PAQR3 and investigated the function of PAQR3 in I/R induced neuronal apoptosis. KEY FINDINGS: PAQR3 expression is markedly increased in the ischemic hemisphere of C57BL/6 mice and PC12 cells after I/R stimulation. Knockdown PAQR3 can attenuate neuronal apoptosis induced by I/R in PC12 cells and exerts neuroprotective effects. PAQR3 deficiency can significantly raise cell viability and suppress LDH leakage under I/R treatment. Silencing PAQR3 attenuates neuronal apoptosis remarkably with fewer TUNEL-positive cells and lower apoptosis rate under I/R treatment. Mechanistically, knockdown of PAQR3 can inhibit the apoptosis pathway through inducing anti-apoptotic proteins and inhibiting pro-apoptotic proteins. Besides, PI3K/AKT signaling suppression with LY294002 abolished the neuroprotective functions induced by silencing PAQR3. SIGNIFICANCE: Our results elucidate that silencing PAQR3 can protect PC12 from OGD/R injury via activating PI3K/AKT pathway. And therefore, provide a novel therapeutic target for the prevention of cerebral I/R injury.

5.
Aging Clin Exp Res ; 2020 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-33219935

RESUMO

BACKGROUND: The visceral adiposity index (VAI) is a newly developing indicator about visceral fat function and insulin resistance. This research aims to assess the association between organ damage and VAI in the community-dwelling elderly Chinese population. METHODS: In total, 3363 elderly participants were recruited between June 2014 and August 2019. VAI was used to measure visceral adipose accumulation, and organ damage was measured with standardized methods, including arterial stiffness, lower extremity atherosclerosis, carotid hypertrophy, left ventricular hypertrophy, micro-albuminuria, and chronic kidney disease. RESULTS: According to multivariable linear regression analysis, VAI was related to carotid-femoral pulse wave velocity (cf-PWV; ß = 0.047, P = 0.024), urine albumin to creatinine ratio (UACR; ß = 3.893, P = 0.008), estimated glomerular filtration rate (eGFR; ß = - 0.526, P = 0.003) and loge(ankle-to-brachial index) (ABI; ß = -0.003, P = 0.024). Using multivariable stepwise logistic regression model, higher VAI was found to be significantly related to cf-PWV > 10 m/s (OR 1.44, [95% CI 1.17-1.78]; Pfor trend < 0.001), and chronic kidney disease (CKD; OR 1.54, [95% CI 1.09-2.20]; Pfor trend = 0.015). CONCLUSIONS: Since higher VAI is related to increased risk of arterial stiffness and CKD, it may serve as a useful index for the assessment of arteriosclerosis and CKD in elderly population. TRIAL REGISTRATION: NSS, NCT02368938.

6.
Opt Lett ; 45(22): 6326-6329, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-33186981

RESUMO

Color-rendering manipulation of solar cells is drawing increasing interest, since the integration of color displaying can promote various advanced applications. However, the dual functionality of high-performance operation and easy processing remain a challenge. Here we propose a colorful perovskite solar cell (PSC) based on purely planar layers. The photonic crystal (PC), which does not interfere with the PSC processing, enables the display of high-purity colors and maintaining the number of PC layers at 4-6. The fabricated PSC with a four-layer PC successfully displays red-green-blue (RGB) colors, with the power-conversion efficiency of 10.94%, 11.01%, and 13.70%, respectively. Further study indicates that by employing a six-layer PC the PSC can obtain excellent color-displaying effect with the color gamut up to 81.8% of the standard RGB. It also shows that the design has a good tolerance to the deviation of layer thickness.

7.
Mol Med Rep ; 22(6): 4567-4578, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33173977

RESUMO

The present study aimed to explore the biological functions and molecular mechanisms of the long non­coding RNA VIM antisense RNA 1 (VIM­AS1) in gastric cancer (GC). The expression of VIM­AS1 was analyzed in tissues from patients with GC and GC cell lines by reverse transcription­quantitative (RT­q)PCR. The relationship between VIM­AS1 expression and overall survival time of patients with GC was also assessed. To determine the biological functions of VIM­AS1, Cell Counting Kit­8 assay, colony formation assay, flow cytometry, wound healing assay and Transwell assay were employed. The targeting relationship among VIM­AS1, microRNA (miR)­8052 and frizzled 1 (FZD1) was verified by the dual luciferase reporter gene assay. The underlying molecular mechanism of VIM­AS1 on GC was determined by RT­qPCR and western blotting. In addition, tumor formation was detected in nude mice. The results of the present study demonstrated that VIM­AS1 was highly expressed in GC tissues and cells. In addition, VIM­AS1 expression was demonstrated to be closely related to the prognosis of patients with GC. Notably, silencing VIM­AS1 inhibited the proliferation, migration and invasion, and enhanced apoptosis of AGS and HGC­27 cells. Silencing VIM­AS1 significantly increased the protein expression levels of cleaved caspase­3, Bax and E­cadherin, but decreased the protein expression levels of Bcl­2, N­cadherin, vimentin, matrix metalloproteinase (MMP)­2, MMP­9, ß­catenin, cyclin D1, C­myc and FZD1. Additionally, silencing VIM­AS1 inhibited tumor growth in nude mice. Cumulatively, the present study demonstrated that VIM­AS1 may promote cell proliferation, migration, invasion and epithelial­mesenchymal transition by regulating FDZ1 and activating the Wnt/ß­catenin pathway in GC.

8.
Ann Diagn Pathol ; 49: 151642, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33142195

RESUMO

BACKGROUND: Many potential biomarkers have been identified and studied for bladder cancer diagnosis. In this study, we investigated the role of a new biomarker, long noncoding RNA (lncRNA) PCAT6, in bladder cancer diagnosis and prognosis. METHODS AND RESULTS: The lncRNA PCAT6 expression profile of BC is analyzed using the Cancer Genome Atlas Urothelial Bladder Carcinoma (TCGA-BLCA) data. PCAT6 expression level in 106 pairs of BC tissues and adjacent normal tissues was detected and compared using qRT-PCR. Then, the association between PCAT6 expression and clinicopathologic indicators of BC was evaluated. Meanwhile, the prognostic value of PCAT6 was tested using Kaplan-Meier analysis. Additionally, loss-of-function assays were used to explore the effect of PCAT6 on the biological function of BC cells. We identified that the expression level of PCAT6 in BC tissue was higher than that in adjacent normal tissues. And the BC patients have higher serum PCAT6 than that in healthy volunteers. In addition, the expression level of PCAT6 was correlated with tumor size (p = 0.005), differentiation (p = 0.018), TNM stage (p = 0.04), lymph nodes metastasis (p = 0.019), and distant metastasis (p = 0.028). Kaplan-Meier analysis showed that BC patients with high PCAT6 expression had shorter overall survival (OS) and progression-free survival (PFS). The loss-of-function results revealed that the proliferation and viability of BC cells in PCAT6 knockdown groups decreased significantly, compared with the negative control groups. CONCLUSION: Our results demonstrated that PCAT6 might be a potential biomarker for diagnosis and prognosis of BC.

9.
Transfusion ; 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33175455

RESUMO

BACKGROUND: Bombay and para-Bombay phenotypes, which arise from gene mutations of α-1,2-fucosyltransferase FUT1, are very rare in Chinese population. A para-Bombay phenotype Chinese individual with two novel FUT1 mutations was reported here. STUDY DESIGN AND METHODS: The peripheral blood and saliva samples of the proband and her family members were collected after informed consent. ABO and H blood group phenotyping was performed by haemagglutination methods. ABO genotype was determined by PCR-SSP kit. A, B, and H antigens in saliva were detected by a hemagglutination inhibition test. Fragments encompassing the full coding region of FUT1 and FUT2 genes were PCR amplified and sequenced. Allelic sequences were validated by cloning and sequencing individual colonies. RESULTS: The serologic reaction results of the proband revealed that A, B, and H antigen were absent on RBCs, but B and H antigen were presented in saliva, and the serum contains anti-H. The proband was assigned as B/O1 by ABO genotyping. Two new heterozygous mutations of FUT1 gene, c.508dupT and c.787A>C, were identified through direct sequencing of PCR-amplified products. TA cloning and sequencing confirmed that two novel mutations were on different alleles. FUT2 gene sequence of the proband is consistent with standard. The other family members of the proband showed normal phenotypes of ABO blood group and their genotypes are consistent with phenotypes. CONCLUSION: Two novel FUT1 alleles, with the previously not reported mutations c.508dupT and c.787C, respectively, are responsible for the para-Bombay phenotype detected in the sample from the proband.

10.
Biomater Sci ; 8(21): 6045-6055, 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33000800

RESUMO

In this paper, a nanocomposite was constructed to achieve improved photodynamic therapy (PDT) via disrupting the redox balance in cancer cells. Firstly, Sb2Se3 nanorods were synthesized as a new photosensitizer, displaying high photothermal conversion efficiency (45.2%) and reactive oxygen species (ROS) production due to the narrow band gap (1.1 eV) and a good NIR response. Moreover, the mechanism was investigated, demonstrating that dissolved O2 and photoinduced electrons manipulated ROS generation. Then, mesoporous silica was coated outside to improve the biocompatibility and to supply abundant space for the anticancer drug (doxorubicin, DOX). The sensitive Se-Se linker was grafted outside via a silane coupling reaction to block DOX molecules in the mesopores. As we know, the Se-Se group is sensitive to GSH, which can induce Se-Se linker bond breakage and targeted drug release due to the high expression of GSH in tumor cells. What is more, the consumption of intracellular GSH can also disrupt the redox balance in cancer cells, which would promote the PDT efficiency. The high-Z element of Sb possesses a high X-ray attenuation coefficient, giving the composite high contrast in CT imaging. This is associated with thermal imaging and multi-therapy (PDT/PTT/chemotherapy) to reveal the potential application to cancer treatment.

11.
Curr Pharm Des ; 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33100194

RESUMO

MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate the expression of targets genes by binding to the 3'-untranslated regions. They play vital roles in diverse biological processes, including the development of hepatic fibrosis (HF). HF is characterized by the accumulation of extracellular matrix (ECM) and hepatic stellate cells (HSCs) are considered a major cell type for producing ECM. Alteration of the HSC phenotype plays a crucial role in the HF pathological process. miRNAs involved in various biological process, such as differentiation, apoptosis, migration, and their relevant signaling pathways, are expressed in HSCs; however, emerging evidence indicates that numerous miRNAs are abnormally expressed in activated HSCs. In this review, we summarize the categorization of miRNAs in HF and describe the relationships among them. We also discuss miRNAs recently discovered to be related to HF, and attempt to find potential miRNAs that may serve as novel biomarkers for use in HF treatment.

12.
Transfusion ; 2020 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-33098315

RESUMO

BACKGROUND: The Am phenotype, which arises from mutations of the α-1,3-N-acetylgalactosaminyltransferase gene, is rare in the Chinese population. The present study focuses on a novel mutation with the Am phenotype in a Chinese individual. STUDY DESIGN AND METHODS: The sample with ABO blood group discrepancy was analyzed by serologic techniques. The full coding and flanking regions of the ABO gene, including the Intron 1 transcription factor-binding site, were identified through direct sequencing of polymerase chain reaction (PCR)-amplified products. PCR products of Exons 6 and 7 were validated to isolate the ABO gene haplotypes by cloning and sequencing individual colonies. The impact of the novel mutation on enzyme function was predicted with Polymorphism Phenotyping algorithm V2 and bioinformatic software programs. RESULTS: The serologic characteristics of ABO blood typing showed the rare Am phenotype. The c.467C/T and c.912C/A heterozygous sites in Exon 7 were identified by direct sequencing analysis. Further TA cloning and sequencing revealed that the patient carried an ABO*O.01.01 allele and a novel ABO*A allele. The new allele sequence had one nucleotide alteration (C>A) at position 912 on the background of the ABO*A1.02 allele. The c.912C>A mutation was predicted to be "probably damaging" and "deleterious" by PolyPhen-2 and PROVEAN algorithms, respectively. CONCLUSION: A novel mutation c.912C>A in α-1,3-N-acetylgalactosaminyltransferase gene resulting in Am phenotype was identified in a Chinese individual.

13.
Mediators Inflamm ; 2020: 6108342, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33013198

RESUMO

Objective: To investigate the relationship between serum interleukin-2 (IL-2) levels and disease activity, absolute numbers of peripheral lymphocyte subsets, autoantibodies, and associated cytokines in patients with rheumatoid arthritis (RA). Methods: This study included 106 patients with RA, evaluated their disease activity (DAS28 score), and divided them into disease remission (DAS28 ≤ 2.6), low disease activity (DAS28 ≤ 3.2), and moderate-high disease activity (DAS28 > 3.2) groups. Flow cytometry was used to detect the absolute numbers of peripheral lymphocyte subpopulations and CD4+ T cell subsets in each group, and serum cytokine levels were measured using cytometric bead array. Results: Serum IL-2 levels in RA patients were positively correlated with disease activity and rheumatoid factor titers (p < 0.001 and p = 0.045, respectively), and multiple regression analysis revealed that serum IL-2 levels were an independent factor affecting disease activity. Serum IL-2 levels were positively correlated with Th17/Treg ratios (p = 0.013). Compared with the remission group, peripheral lymphocyte and CD4+ T lymphocyte subsets in patients with active RA decreased to varying degrees; however, the numbers of peripheral natural killer (NK) cells were significantly higher in the moderate-high disease activity group than in the remission (p = 0.046) and low disease activity (p = 0.020) groups; the percentages of NK cells had the same trend. In addition, the number and percentage of NK cells were positively correlated with serum IL-2 levels (p = 0.018 and p = 0.006, respectively). Conclusions: In RA patients, serum IL-2 levels were not only correlated with patients' disease activity and autoantibody levels but were also involved in their Th17/Treg immune imbalance. In addition, in patients with active RA, NK cell levels were abnormally elevated, possibly due to high serum levels of IL-2.

14.
Circulation ; 142(22): 2138-2154, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32933333

RESUMO

BACKGROUND: Concentric and eccentric cardiac hypertrophy are associated with pressure and volume overload, respectively, in cardiovascular disease both conferring an increased risk of heart failure. These contrasting forms of hypertrophy are characterized by asymmetrical growth of the cardiac myocyte in mainly width or length, respectively. The molecular mechanisms determining myocyte preferential growth in width versus length remain poorly understood. Identification of the mechanisms governing asymmetrical myocyte growth could provide new therapeutic targets for the prevention or treatment of heart failure. METHODS: Primary adult rat ventricular myocytes, adeno-associated virus (AAV)-mediated gene delivery in mice, and human tissue samples were used to define a regulatory pathway controlling pathological myocyte hypertrophy. Chromatin immunoprecipitation assays with sequencing and precision nuclear run-on sequencing were used to define a transcriptional mechanism. RESULTS: We report that asymmetrical cardiac myocyte hypertrophy is modulated by SRF (serum response factor) phosphorylation, constituting an epigenomic switch balancing the growth in width versus length of adult ventricular myocytes in vitro and in vivo. SRF Ser103 phosphorylation is bidirectionally regulated by RSK3 (p90 ribosomal S6 kinase type 3) and PP2A (protein phosphatase 2A) at signalosomes organized by the scaffold protein mAKAPß (muscle A-kinase anchoring protein ß), such that increased SRF phosphorylation activates AP-1 (activator protein-1)-dependent enhancers that direct myocyte growth in width. AAV are used to express in vivo mAKAPß-derived RSK3 and PP2A anchoring disruptor peptides that block the association of the enzymes with the mAKAPß scaffold. Inhibition of RSK3 signaling prevents concentric cardiac remodeling induced by pressure overload, while inhibition of PP2A signaling prevents eccentric cardiac remodeling induced by myocardial infarction, in each case improving cardiac function. SRF Ser103 phosphorylation is significantly decreased in dilated human hearts, supporting the notion that modulation of the mAKAPß-SRF signalosome could be a new therapeutic approach for human heart failure. CONCLUSIONS: We have identified a new molecular switch, namely mAKAPß signalosome-regulated SRF phosphorylation, that controls a transcriptional program responsible for modulating changes in cardiac myocyte morphology that occur secondary to pathological stressors. Complementary AAV-based gene therapies constitute rationally-designed strategies for a new translational modality for heart failure.

15.
J Sep Sci ; 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32990401

RESUMO

Discovering marker components of traditional Chinese medicine formulas is challenging because of the hundreds of components they inherently contain. This study first proposed a reliable and validated method for the comprehensive profiling of chemical constituents in Honghua Xiaoyao tablet by using high-performance liquid chromatography coupled with mass spectrometry. After searching within the in-house library, a total of 55 constituents were unambiguously characterized or tentatively identified through reference standards and by comparing mass spectrometry data with literature values. Quantitative analysis of 14 compounds, which were selected as the quality marker components based on a serum pharmacochemistry study, has been performed by triple-quardrupole mass spectrometry technique. Multiple chemometric methods, including principal components analysis and hierarchical cluster analysis, were subsequently used to analyze the quantitative results, classify samples from three manufacturers, and distinguish the analytical markers. In method validation results, 14 quality marker compounds have shown good linearity (R2 ≥ 0.9965) with a relative wide concentration range and acceptable recovery at 98.39-102.46%. The proposed approach provides the chemical evidence for revealing the material basis of Honghua Xiaoyao tablet, and establishes a reliable statistical analysis-based strategy of quality marker investigation for controlling its quality.

17.
Small ; 16(41): e2002435, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32954651

RESUMO

Infection with live-attenuated vaccines always inevitably induces side effects that reduce their safety. This study suggests a concept of magnetic virus produced by genetically modifying viral surfaces with Fe3 O4 nanoparticles (NPs) to control their tropisms. An iron-affinity peptide is designed to be displayed on the viral surface protein (VP1) of human enterovirus type 71 (EV71), a typical nonenveloped picornavirus, as the model. The modified EV71 can self-bind with Fe3 O4 NPs under physiological conditions, resulting in novel EV71-Fe3 O4 hybrid materials. This rationally engineered EV71 with Fe3 O4 retains its original biological infectivity, but its tropism can be precisely controlled by magnetism. Both in vitro and in vivo experiments demonstrate that EV71-Fe3 O4 can infect only a desired area within the limit of the applied magnetic field, which effectively reduces its pathological damage. More importantly, this characteristic of EV71 can be inherited due to the gene-induced coassembly of viruses and NPs. This achievement provides a proof of concept in virus vaccine improvement by a combination of gene modification and material incorporation, leading to great potential for biomedical developments.

18.
Cardiol Young ; 30(10): 1532-1534, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32959745

RESUMO

CHD is closely related to respiratory system diseases (Mok Q, Front Pediatr 2017; 5: 2296-2360). Flexible fibreoptic bronchoscopy will diagnose anatomical lesions of the trachea and perform interventions at the same time for children with indications. We report a case of pulmonary artery sling with severe tracheostenosis in a 11-month-old boy. Tracheal stents were placed with good prognosis.

19.
Int Heart J ; 61(5): 936-943, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32879265

RESUMO

On the basis of radiofrequency ablation of atrial fibrillation (AF), some studies suggested that early recurrences of atrial tachyarrhythmia (ERATs) were associated with late AF recurrence (LAFR), and some also suspected and challenged the current recommended 90 day blanking period. We aim to evaluate the impact of ERAT on long-term success and to determine the optimum blanking period after AF ablation using second-generation cryoballoon (sg-CB). From August 2016 to October 2018, 369 consecutive patients who successfully underwent initial AF ablation using sg-CB at the Fuwai Hospital were finally enrolled. All patients were followed up no less than 12 months. Receiver operating characteristic curve analysis was used to determine the optimum blanking period after AF ablation. There were 62 (16.8%) who experienced ERAT. After a median follow-up of 615 days, 74.5% were free of LAFR after the 90 day blanking period. Incidence of freedom from LAFR during the long-term follow-up was markedly lower in patients with ERAT than in those without ERAT (27.4% versus 84.0%; log-rank P < 0.001). Furthermore, only ERAT (HR 8.579; 95% CI 5.604-13.133; P < 0.001) was significantly associated with an increased risk of LAFR after adjusting for other factors. The optimum cut-off time point for the blanking period was 21.5 days (sensitivity: 71.1%, specificity: 94.1%). In conclusion, ERAT was an independent predictor of LAFR after AF ablation using sg-CB. Based on our findings, blanking period was advised to be shorten to 21.5 days or about 3 weeks instead of 90 days after CB ablation.


Assuntos
Fibrilação Atrial/cirurgia , Criocirurgia , Veias Pulmonares/cirurgia , Recidiva , Falha de Tratamento , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Tempo
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