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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121942, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36209715

RESUMO

A surface-enhanced Raman spectroscopy (SERS) method for the determination of isepamicin (ISE) using silver nanoparticles (AgNPs) protected by bovine serum albumin (BSA) and modified by α-Fe2O3 as an efficient substrate was established. The synthesized substrate was characterized and verified by transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis), and fourier transform infrared spectroscopy (FT-IR). The conditions affecting the Raman signal of ISE were optimized by single factor and response surface experiments. Under optimized conditions, a standard curve ISERS = 43.08c + 63598.69 (c: nmol/L) with a linear relationship (r = 0.9976) was established between the SERS intensity and ISE concentration in the range of 20.00 - 2000.00 nmol/L. The limit of detection (LOD) for ISE was 16.58 nmol/L (S/N = 3). The recovery of ISE in the samples was 96.29 % - 104.12 %, with relative standard deviation (RSD) was 1.53 % - 3.43 % (n = 5). The SERS method was reliable and satisfactory for the quantitative analysis of ISE at low concentration.


Assuntos
Nanopartículas Metálicas , Prata , Prata/química , Nanopartículas Metálicas/química , Soroalbumina Bovina , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman/métodos
2.
Bioact Mater ; 21: 175-193, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36093328

RESUMO

Mandibular defect becomes a prevalent maxillofacial disease resulting in mandibular dysfunctions and huge psychological burdens to the patients. Considering the routine presence of oral contaminations and aesthetic restoration of facial structures, the current clinical treatments are however limited, incapable to reconstruct the structural integrity and regeneration, spurring the need for cost-effective mandibular tissue engineering. Hydrogel systems possess great merit for mandibular reconstruction with precise involvement of cells and bioactive factors. In this review, current clinical treatments and distinct mode(s) of mandible formation and pathological resorption are summarized, followed by a review of hydrogel-related mandibular tissue engineering, and an update on the advanced fabrication of hydrogels with improved mechanical property, antibacterial ability, injectable form, and 3D bioprinted hydrogel constructs. The exploration of advanced hydrogel systems will lay down a solid foundation for a bright future with more biocompatible, effective, and personalized treatment in mandibular reconstruction.

3.
Neural Regen Res ; 18(5): 1062-1066, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36254994

RESUMO

Multi-target neural circuit-magnetic stimulation has been clinically shown to improve rehabilitation of lower limb motor function after spinal cord injury. However, the precise underlying mechanism remains unclear. In this study, we performed double-target neural circuit-magnetic stimulation on the left motor cortex and bilateral L5 nerve root for 3 successive weeks in a rat model of incomplete spinal cord injury caused by compression at T10. Results showed that in the injured spinal cord, the expression of the astrocyte marker glial fibrillary acidic protein and inflammatory factors interleukin 1ß, interleukin-6, and tumor necrosis factor-α had decreased, whereas that of neuronal survival marker microtubule-associated protein 2 and synaptic plasticity markers postsynaptic densification protein 95 and synaptophysin protein had increased. Additionally, neural signaling of the descending corticospinal tract was markedly improved and rat locomotor function recovered significantly. These findings suggest that double-target neural circuit-magnetic stimulation improves rat motor function by attenuating astrocyte activation, thus providing a theoretical basis for application of double-target neural circuit-magnetic stimulation in the clinical treatment of spinal cord injury.

4.
Sci Total Environ ; 856(Pt 2): 159191, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36195150

RESUMO

Soil moisture (SM) is essential for controlling terrestrial carbon uptake, as it directly provides moisture for photosynthesis, especially in arid and semiarid regions. We selected the arid and semiarid Ili River basin (IRB) of Xinjiang as the study area, and investigated the spatial and temporal characteristics and interrelationships with SM and photosynthesis from 2000 to 2018 using the ERA5 products and solar-induced chlorophyll fluorescence (SIF). SM and photosynthesis showed a decreasing trend during the study period. Compared with those in spring and autumn, the variation of summer SM and SIF was more consistent with the interannual variation. Anomaly analysis showed that negative SM anomalies were most profound in 2012-2015, 2008, and 2014. Additionally, we quantified the effect of seasonal SM deficits on photosynthesis by performing model-based experiments. The results indicated that the gross primary productivity (GPP) simulated by the P-model could capture the characteristics of photosynthesis in the IRB, which had a high correlation with SIF (R2 = 0.82, p < 0.001). In 2012-2015, 2008, and 2014, SM deficits caused more GPP reduction in the summers than in the springs or the autumns. The trends were mainly visible in the northern IRB, where GPP was below 40 % of the multi-year mean, and SM was below 23 %. GPP decreased more significantly in grassland than in the forest under the influence of SM deficit. This study reveals seasonal differences in the effects of SM deficit on photosynthesis and emphasizes that the summer SM deficit was the main factor responsible for decreases in GPP in the IRB during the study period. These findings contribute to a better understanding of the relationships between photosynthesis and environmental factors, and provide a reference for an accurate assessment of the regional carbon cycle.


Assuntos
Clorofila , Solo , Estações do Ano , Rios , Ecossistema , Fluorescência , Fotossíntese
5.
J Ment Health ; : 1-8, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36322512

RESUMO

BACKGROUND: Few studies have been conducted to explore medication non-adherence in persons with schizophrenia (PWS) and its influencing factors in rural China. This study aimed to investigate the medication non-adherence and its influencing factors among PWS in rural China. METHODS: A total of 269 PWS and their family caregivers in Xinjin district, Chengdu, China were investigated on medication adherence and related factors. Logistic regression was employed to identify the influencing factors. RESULTS: The results showed that 37.6% of PWS had medication non-adherence. PWS living with family caregivers had significantly lower rate of medication non-adherence (34.7%) than those not living with family caregivers (60.0%) (p < 0.01). Family caregivers' affiliate stigma of mental illness, knowledge of mental illness and self-esteem were significantly related to patients' medication non-adherence (p < 0.05). PWS' employment status, living with family caregiver, present mental status and social support were significantly related to medication adherence. CONCLUSION: This study shows medication non-adherence is severe among PWS in rural China. Both patient- and family-related factors affect patients' medication adherence seriously. Except improving patients' treatment and mental status, development of family caregiving, social support network and intervention on reducing stigma of mental illness should be crucial for enhancing PWS' medication adherence.

6.
Front Microbiol ; 13: 1042117, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36329849

RESUMO

Lacticaseibacillus paracasei SMN-LBK, which was isolated in Xinjiang, has been shown to be a probiotic strain and used as the auxiliary starter for dairy fermentation. Comparative genomic analysis was performed to investigate the metabolic preference and ethanol tolerance mechanisms of L. paracasei SMN-LBK. The results of comparative genomics showed that L. paracasei strains had high conservation and genetic diversity. SMN-LBK encoded various genes related to carbohydrate and amino acid metabolism pathways, which endow this strain with good fermentation potential. In addition, 6 CRISPR sequences and 8 cas proteins were found in SMN-LBK, and these could play vital roles in the immune system. Furthermore, a unique cluster of potential secondary metabolism genes related to bacteriocins was detected in the genome of SMN-LBK, and this could be important for the preservation of fermented foods. Multiple genes related to alcohol tolerance were also identified. In conclusion, our study explained the traits that were previously demonstrated for SMN-LBK as phenotypes and provided a theoretical basis for the application of SMN-LBK in the food industry.

7.
J Inflamm Res ; 15: 6021-6030, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36330168

RESUMO

Aim: The development of microsurgery has greatly advanced vascularized composite allotransplantation (VCA). However, like organ transplantation, VCA is also limited by acute rejection, and concerns regarding long-term survival and function of the transplanted graft. Therefore, it is necessary to elucidate the molecular mechanisms underlying acute rejection caused by VCA, in order to improve patient survival. Methods: Firstly, we used Brown Norway rats and Lewis rats to construct animal model of VCA. Regularly record the appearance changes of all subjects. Specimens were collected for histological examination, microRNAs (miRNAs) sequencing and RT-qPCR verification when acute immune rejection occurred. Then, bioinformatics analysis was employed to predict miRNA related molecules and pathway information. Finally, differentially expressed miRNAs were tested and verified. Results: MiRNAs are small non coding RNA transcripts that regulate gene expression at the post-transcriptional level. Studies have shown that miRNAs are involved in immune regulation and several miRNAs have been identified that are potential diagnostic and prognostic biomarkers of acute rejection. In this study, we found that the expression levels of rno-miR-21-5p, rno-miR-340-5p, rno-miR-1-3p and rno-miR-195-5p are significantly associated with acute rejection following VCA. Conclusion: This miRNA signature can potentially an auxiliary diagnostic indicator of rejection, which can help clinicians adjust the immunosuppressive program in time during acute rejection.

8.
Quant Imaging Med Surg ; 12(11): 5239-5250, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36330175

RESUMO

Background: Identifying epidermal growth factor receptor (EGFR) mutations in lung adenocarcinoma (LADC) is vital for treatment decision-making. This study aimed to establish a convenient and noninvasive nomogram prediction model based on 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) imaging and clinical features to predict EGFR mutation status in patients with LADC. Methods: A total of 274 patients (male 130, female 144, median age 65 years) were enrolled in this retrospective study. Imaging data from 18F-FDG PET/CT and clinical information were analyzed, with the Mann-Whitney U test, Student's t-test, and chi-square test used to compare categorical or continuous covariates as appropriate. Logistic regression analyses were performed to identify independent variables associated with EGFR mutation status, from which the nomogram prediction model was constructed. Leave-one-out cross-validation was performed, and the discrimination ability and calibration of the nomogram were assessed by calculating the area under the curve of the receiver operating characteristic curve and the calibration curve. The clinical net benefit of the nomogram was evaluated. Results: Of the 274 patients, 143 (52.2%) had EGFR mutations. Female sex [odds ratios (OR): 2.64, 95% confidence interval (CI): 1.29-5.45, P=0.008], non-smoking status (OR: 2.78, 95% CI: 1.30-5.88, P=0.008), mean standardized uptake value ≤9.23 (OR: 2.44, 95% CI: 1.35-4.55, P=0.004), metabolic tumor volume ≤17.72 cm3 (OR: 5.00, 95% CI: 2.38-12.50, P<0.001) and the presence of pleural retraction (OR: 1.88, 95% CI: 1.05-3.40, P=0.034) were independent predictors for EGFR mutations in LADCs. The nomogram based on these risk factors showed good predictive efficacy, with an area under the curve of 0.805 (95% CI: 0.753-0.857), a sensitivity of 90.2%, a specificity of 59.5% and an accuracy of 73.0%. Conclusions: The nomogram prediction model incorporating sex, smoking status, mean standardized uptake value, metabolic tumor volume, and the presence of pleural retraction could effectively discriminate EGFR-mutant from wild-type LADCs.

9.
Med Phys ; 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36323626

RESUMO

BACKGROUND: In recent years, Cone-beam computed tomography (CBCT) has played an important role in medical imaging. However, the applications of CBCT are limited due to the severe scatter contamination. Conventional Monte Carlo (MC) simulation can provide accurate scatter estimation for scatter correction, but the expensive computational cost has always been the bottleneck of MC method in clinical application. PURPOSE: In this work, a MC simulation method combined with a variance reduction technique called correlated sampling is proposed for fast iterative scatter correction. METHODS: Correlated sampling exploits correlation between similar simulation systems to reduce the variance of interest quantities. Specifically, conventional MC simulation is first performed on the scatter-contaminated CBCT to generate the initial scatter signal. In the subsequent correction iterations, scatter estimation is then updated by applying correlated MC sampling to the latest corrected CBCT images by reusing the random number sequences of the task-related photons in conventional MC. Afterwards, the corrected projections obtained by subtracting the scatter estimation from raw projections are utilized for FDK reconstruction. These steps are repeated until an adequate scatter correction is obtained. The performance of the proposed framework is evaluated by the accuracy of the scatter estimation, the quality of corrected CBCT images and efficiency. RESULTS: Overall, the difference in MAPE between scatter estimation with and without correlated sampling is 0.25% for full-fan case and 0.34% for half-fan case, respectively. In simulation studies, scatter artifacts are substantially eliminated, where the MAE value is reduced from 15 to 2 HU in full-fan case and from 53 to 13 HU in half-fan case. SPR is reduced to 0.02 for full-fan and 0.04 for half-fan, respectively. In phantom study, the CNR is increased by a factor of 1.63 and the contrast is increased by a factor of 1.77. As for clinical studies, the CNR is improved by 11% and 14% for half-fan and full-fan, respectively. The contrast after correction is increased by 19% for half-fan and 44% for full-fan. Furthermore, RMSE is also effectively reduced, especially from 78 to 4 HU for full-fan. Experimental results demonstrate that the FOM is improved between 23 and 43 folds when using correlated sampling. The proposed method takes less than 25 seconds for the whole iterative scatter correction process. CONCLUSIONS: The proposed correlated sampling-based MC simulation method can achieve fast and accurate scatter correction for CBCT, making it suitable for real-time clinical use. This article is protected by copyright. All rights reserved.

10.
Plant Physiol ; 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377782

RESUMO

Plant flavonoids are valuable natural antioxidants. Sweet potato (Ipomoea batatas) leaves are rich in flavonoids, regenerate rapidly, and can adapt to harsh environments, making them an ideal material for flavonoid biofortification. Here, we demonstrate that the B-box (BBX) family transcription factor IbBBX29 regulates the flavonoid contents and development of sweet potato leaves. IbBBX29 was highly expressed in sweet potato leaves and significantly induced by auxin (IAA). Overexpression of IbBBX29 contributed to a 21.37-70.94% increase in leaf biomass, a 12.08-21.85% increase in IAA levels, and a 31.33-63.03% increase in flavonoid accumulation in sweet potato, whereas silencing this gene produced opposite effects. Heterologous expression of IbBBX29 in Arabidopsis (Arabidopsis thaliana) led to a dwarfed phenotype, along with enhanced IAA and flavonoid accumulation. RNA-seq analysis revealed that IbBBX29 modulates the expression of genes involved in the auxin signaling and flavonoid biosynthesis pathways. ChIP-qPCR and EMSA indicated that IbBBX29 targets key genes of auxin signaling and flavonoid biosynthesis to activate their expression by binding to specific T/G-boxes in their promoters, especially those adjacent to the transcription start site. Moreover, IbBBX29 physically interacted with developmental and phenylpropanoid biosynthesis-related proteins, such as MADS-box protein IbAGL21 and MYB308-like protein IbMYB308L. Finally, overexpressing IbBBX29 also increased flavonoid contents in sweet potato storage roots. These findings indicate that IbBBX29 plays a pivotal role in regulating IAA-mediated leaf development and flavonoid biosynthesis in sweet potato and Arabidopsis, providing a candidate gene for flavonoid biofortification in plants.

13.
Molecules ; 27(21)2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36364129

RESUMO

The goal of this work was to use the GC-MS technique to explore the chemical components of Artemisia giraldii Pamp essential oil (AgEo) and to uncover its antibacterial activity, specifically the antibacterial mechanism of this essential oil. There were a total of 63 chemical constituents in the AgEo, monoterpenes (10.2%) and sesquiterpenes (30.14%) were found to be the most common chemical components, with camphor (15.68%) coming in first, followed by germacrene D. (15.29%). AgEo displayed significant reducing power and good scavenging ability on hydroxyl radicals, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals, and 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) radicals, according to antioxidant data. The diameter of the inhibition zone (DIZ) of AgEo against S. aureus and E. coli was (14.00 ± 1.00) mm and (16.33 ± 1.53) mm, respectively; the minimum inhibitory concentration (MIC) of AgEo against E. coli and S. aureus was 3 µL/mL and 6 µL/mL, respectively; and the minimum bactericidal concentration (MBC) of AgEo against E. coli and S. aureus was 6 µL/mL and 12 µL/mL, respectively. The antibacterial curve revealed that 0.5MIC of AgEo may delay bacterial growth while 2MIC of AgEo could totally suppress bacterial growth. The relative conductivity, alkaline phosphatase (AKP) activity, and protein concentration of the bacterial suspension were all higher after the AgEo treatment than in the control group, and increased as the essential oil concentration was raised. In addition, the cell membrane ruptured and atrophy occurred. The study discovered that AgEo is high in active chemicals and can be used as an antibacterial agent against E. coli and S. aureus, which is critical for AgEo's future research and development.


Assuntos
Artemisia , Óleos Voláteis , Óleos Voláteis/química , Artemisia/química , Staphylococcus aureus , Escherichia coli , Padrões Moleculares Associados a Patógenos , Testes de Sensibilidade Microbiana , Antibacterianos/química , Antioxidantes/farmacologia , Antioxidantes/química
14.
Sensors (Basel) ; 22(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36365820

RESUMO

Impact force is the most common form of load which acts on engineering structures and presents a great hidden risk to the healthy operation of machinery. Therefore, the identification or monitoring of impact forces is a significant issue in structural health monitoring. The conventional optimisation scheme based on inversion techniques requires a significant amount of time to identify random impact forces (impact force localisation and time history reconstruction) and is not suitable for engineering applications. Recently, a pattern recognition method combined with the similarity metric, PRMCSM, has been proposed, which exhibits rapidity in practical engineering applications. This study proposes a novel scheme for identifying unknown random impact forces which hybridises two existing methods and combines the advantages of both. The experimental results indicate that the localisation accuracy of the proposed algorithm (100%) is higher than that of PRMCSM (92%), and the calculation time of the hybrid algorithm (179 s) for 25 validation cases is approximately one nineteenth of the traditional optimisation strategy (3446 s).


Assuntos
Algoritmos , Aço , Fenômenos Mecânicos
15.
RSC Adv ; 12(47): 30248-30252, 2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36349148

RESUMO

A highly efficient method for the facile access of isoquinolines and isoquinoline N-oxides via a Cu(i)-catalyzed intramolecular cyclization of (E)-2-alkynylaryl oxime derivatives in water has been developed. This protocol was performed under simple and mild conditions without organic solvent, additives or ligands. By switching on/off a hydroxyl protecting group of oximes, the selective N-O/O-H cleavage could be triggered, delivering a series of isoquinolines and isoquinoline N-oxides, respectively, in moderate to high yields with good functional group tolerance and high atom economy. Moreover, the practicality of this method was further demonstrated by the total synthesis of moxaverine in five steps.

16.
Cell Oncol (Dordr) ; 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36350496

RESUMO

PURPOSE: AKT hyperactivation drives malignant phenotypes in lung cancer via promoting tumor cell proliferation and survival. However, the relationship between dysregulation of cell cycle progression and AKT1 kinase activity is still not clear. METHODS: Following the expression level of PKMYT1 in lung cancer, we performed cell proliferation, migration, invasion, and xenograft assays to determine the function of PKMYT1. We used RNA-seq to explore the anti-tumor mechanism of PKMYT1 and examined the effect of PKMYT1 on AKT1 activity. RESULTS: In this study, we report that PKMYT1 is downregulated in lung adenocarcinoma (LUAD) tissues and its low expression predicts a poor prognosis in LUAD patients. PKMYT1 exerts potent tumor-suppressive functions in LUAD cells by inhibiting AKT1 activation and thereby repressing cell cycle progression, which depends on its tyrosine and threonine protein kinase activity. Interestingly, PKMYT1 could directly bind AKT1 to abrogate AKT1 activation. Moreover, silencing AKT1 and inhibitors targeting the AKT pathway effectively reverse the promoting effects of PKMYT1 knockdown on proliferation, migration and invasion of LUAD cells. CONCLUSION: This work reveals the anti-tumor effect of PKMYT1 in LUAD and provides evidence to clarify the dual roles of PKMYT1 in tumor progression. Moreover, our findings broaden the current understandings on AKT1 activation and identify PKMYT1 as a potential negative regulator of AKT1 kinase activity, providing further insights into targeting the AKT pathway in LUAD.

17.
Ann Palliat Med ; 11(10): 3193-3202, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36367001

RESUMO

BACKGROUND: The mortality rate is high in patients with acute kidney injury (AKI). Hyperglycemia and hypoglycemia alone can increase the morbidity and mortality of patients with AKI. Up to now, no relevant studies have analyzed the relationship between different blood glucose levels and mortality in AKI patients. Therefore, exploring the relationship between baseline blood glucose level and 30-day mortality in patients with AKI can provide early warning information for disease prognosis and provide reference basis for reasonable level of blood glucose control. METHODS: This retrospective cohort study was obtained from the Medical Information Mart for Intensive Care III (MIMIC-III) database. Patients had experienced AKI within 48 hours of admission. AKI was diagnosed according to the Kidney Disease: Improving Global Outcomes (KDIGO) guidelines. Data on patients' baseline blood glucose level on admission was retrieved, and the outcome indicator was 30-day mortality. A multivariate Cox regression analysis and smoothed curve fitting were used to assess the relationship between the baseline blood glucose level and 30-day mortality. The covariates used for adjustment were those in the patient's baseline data. RESULTS: A total of 14,449 AKI patients were screened. The overall 30-day mortality rate was 17.6%. Patients with blood glucose levels of 6.36-7.35 mmol/L on admission had the lowest 30-day mortality risk. The multivariate Cox regression model and smoothed curve fitting revealed a U-shaped relationship between the baseline blood glucose level and 30-day mortality after adjusting all the covariables of the baseline data. The inflection point occurred at 5.52 mmol/L. The effect size was 0.773 [hazards ratio (HR) =0.773; 95% confidence interval (CI): 0.614-0.975, P=0.030] on the left side of the inflection point, and 1.077 (HR =1.077; 95% CI: 1.059-1.097, P<0.001) on the right side. CONCLUSIONS: The blood glucose of patients with AKI should be controlled at a reasonable level and should not be lower than 5.52 mmol/L, and the optimal control level needs further study. The limitation of this study is that there are some confounding factors in the retrospective study.


Assuntos
Injúria Renal Aguda , Glicemia , Humanos , Estudos Retrospectivos , Fatores de Risco , Cuidados Críticos , Prognóstico
18.
Adv Sci (Weinh) ; : e2204925, 2022 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-36372543

RESUMO

Mechanoluminescence, a smart luminescence phenomenon in which light energy is directly produced by a mechanical force, has recently received significant attention because of its important applications in fields such as visible strain sensing and structural health monitoring. Up to present, hundreds of inorganic and organic mechanoluminescent smart materials have been discovered and studied. Among them, strontium-aluminate-based materials are an important class of inorganic mechanoluminescent materials for fundamental research and practical applications attributed to their extremely low force/pressure threshold of mechanoluminescence, efficient photoluminescence, persistent afterglow, and a relatively low synthesis cost. This paper presents a systematic and comprehensive review of strontium-aluminate-based luminescent materials' mechanoluminescence phenomena, mechanisms, material synthesis techniques, and related applications. Besides of summarizing the early and the latest research on this material system, an outlook is provided on its environmental, energy issue and future applications in smart wearable devices, advanced energy-saving lighting and displays.

19.
J Thorac Dis ; 14(10): 3983-3991, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36389322

RESUMO

Background: As a novel alternative to the conventional minimally invasive esophagectomy (MIE), more minimally invasive single-port laparoscopic retrograde 3-step gastric mobilization (SLRM) for esophageal reconstruction during MIE to treat esophageal cancer was attempted by our department. This study explored the preliminary clinical outcomes and feasibility of this innovative surgery. Methods: The data of 120 patients who had undergone SLRM combined with 4-port thoracoscopic McKeown esophagectomy for their esophageal cancers from March 2020 to November 2021 were reviewed. Gastric mobilization with abdominal lymph node dissection was performed via SLRM. The clinical characteristics and short-term outcomes were retrospectively analyzed. The data of operating time, blood loss, harvested lymph nodes, postoperative hospital stay and complications are presented as the mean and standard deviation. Results: A total of 120 patients underwent R0 resection without conversion to open surgery. The mean times for the thoracic procedure, abdominal procedure, and total operation were 43±6, 60±18, and 195±20 min, respectively. The numbers of mediastinal and abdominal lymph nodes harvested were 13.2±2.7, and 10.2±2.5, respectively. Postoperative pneumonia occurred in 10 patients (8.3%). Anastomotic leakage occurred in 3 patients (2.5%). Temporary vocal cord paralysis was reported in 20 patients (16.6%). The mean length of hospital stay was 8.5±4.6 days. Conclusions: SLRM is a technically feasible and safe treatment for patients with esophageal cancer. It can be considered an alternative method for patients, especially those with obesity and gastric distension.

20.
Chemistry ; 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36401589

RESUMO

The relationship between interface structure (e.g. the facet of the solid phase and the configuration of solvation) and the reactivity of corresponding electrode is a critical issue in electrochemistry. Compared to macroscopic electrode measurements, electrochemical methods established on the single-particle scale have advantages in establishing the structure-property relationship. In recent years, great achievements have been made in the electrochemical energy storage and electrocatalysis for understanding the evolution and kinetics of electrodes by employing single-particle measurements. This concept aims to provide an overview of the update of single-particle measurements in the related electrochemical processes. Furthermore, the challenges and prospects for the development and application of single-particle measurements are also discussed.

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