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1.
Dig Dis Sci ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32006210

RESUMO

BACKGROUND: Currently there is no consensus on the optimal management of small-for-size syndrome following liver transplantation. Here we describe a technique to alleviate portal hypertension and improve the hepatocyte reperfusion in small-for-size liver transplantation in a Lewis rat model. METHODS: The rats underwent trans-portal vein intra-hepatic portosystemic shunt using a self-developed porous conical tube (TPIPSS: Fig. 1) on small-for-size liver transplants (SFS) with right lobe graft. The treatment effect was evaluated by comparing hemodynamic parameters, morphological changes, serum parameters, ET-1 and eNOS expression, hepatocyte proliferation and apoptosis, CYP3A2 levels, postoperative complications, and survival between the two groups with SFS liver transplants. RESULTS: Porous conical prosthesis prolonged the filling time of small-for-size grafts. Moreover, grafts with TPIPSS showed a lower portal vein pressure, improved microcirculatory flow, alleviated histological changes, decreased ET-1 and increased eNOS expressions, and significantly less damage to liver function comparing to grafts without TPIPSS. Mean survival and overall 30-day survival were significantly higher in the TPIPSS group. CONCLUSIONS: These results demonstrate that porous conical tube as trans-portal vein intra-hepatic portosystemic shunt device is an effective way to alleviate portal vein hypertension and improve hepatocyte reperfusion after small-for-size liver transplantation.

2.
Neurosci Lett ; 722: 134862, 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32105766

RESUMO

Dishevelled-1(DVL-1) has been reported associated with the regulation of cell polarity and neuronal function. However, the effect of DVL-1 in cerebral ischemia-reperfusion injury of rats remains poorly understood. In this study, we give evidence that the level of DVL-1 is increased after a middle cerebral artery occlusion/reperfusion model (MCAO) in rats, with a peak at 12 h. On the side, knockdown of DVL-1 may relieve I/R damage and restrain apoptosis after MCAO model in rats. In the part of mechanism, DVL-1 could regulate apoptosis through NF-κB. These results suggest that DVL-1 may be a potential target in I/R injury in rats.

3.
J Environ Manage ; 262: 110299, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-32094105

RESUMO

Cobalt-based Zeolitic imidazolate frameworks (ZIFs) have shown a great potential for radical production by activating peroxymonosulfate (PMS). However, improve the stability of ZIFs in the reaction remains a significant challenge. In this work, ZIF-67 was synthesized and protected by coating with a layer of silica, furthermore, the yolk-shell ZIFs@SiO2 was carbonized under inner gas to obtain the Co containing carbon. When the above samples were applied for catalytic degradation of Rhodamine B (RhB) in the presence of PMS, both of them shows similar performance, with higher RhB removal efficiency and stability than that of pure ZIF-67. Additionally, factors affecting the PMS activation such as catalyst and PMS dosage and solution pH were also investigated. Radical quenching tests and electron paramagnetic resonance (EPR) revealed that 1O2 was the dominant active species involving in the degradation process. Finally, the reusability of the catalysts was studied and the spent catalysts were analyzed. Overall, the results provide insights into synthesis of yolk-shell ZIFs@SiO2 catalyst with enhanced performance for the degradation of organic pollutants from effluent.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32061788

RESUMO

Electroconvulsive therapy (ECT) can induce fast remission of depression but still retain the residual functional impairments in major depressive disorder (MDD) patients. To delineate the different functional circuits of effective antidepressant treatment and residual functional impairments is able to better guide clinical therapy for depression. Herein, voxel-level whole brain functional connectivity homogeneity (FcHo), functional connectivity, multivariate pattern classification approaches were applied to reveal the specific circuits for treatment response and residual impairments in MDD patients after ECT. Increased FcHo values in right dorsomedial prefrontal cortex (dmPFC) and left angular gyrus (AG) and their corresponding functional connectivities between dmPFC and right AG, dorsolateral prefrontal cortex (dlPFC), superior frontal gyrus, precuneus (Pcu) and between left AG with dlPFC, bilateral AG, and left ventrolateral prefrontal cortex in MDD patients after ECT. Moreover, we found decreased FcHo values in left middle occipital gyrus (MOG) and lingual gyrus (LG) and decreased functional connectivities between MOG and dorsal postcentral gyrus (PCG) and between LG and middle PCG/anterior superior parietal lobule in MDD patients before and after ECT compared to healthy controls (HCs). The increased or normalized FcHo and functional connections may be related to effective antidepressant therapy, and the decreased FcHo and functional connectivities may account for the residual functional impairments in MDD patients after ECT. The different change patterns in MDD after ECT indicated a specific brain circuit supporting fast remission of depression, which was supported by the following multivariate pattern classification analyses. Finally, we found that the changed FcHo in dmPFC was correlated with changed depression scores. These results revealed a specific functional circuit supporting antidepressant effects of ECT and neuroanatomical basis for residual functional impairments. Our findings also highlighted the key role of dmPFC in antidepressant and will provide an important reference for depression treatment.

5.
Dis Markers ; 2020: 8393075, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32076466

RESUMO

Purpose: CD89 (FcαRI), the receptor of IgA, can shed from cells to form complexes with IgA in serum and is supposed to participate in the pathogenesis of IgA nephropathy (IgAN). There are contradictory results on their utility in clinical practice. This study is aimed at investigating whether sCD89-IgA complexes can help in the diagnosis or evaluation of the disease. Methods: A sandwich ELISA was established using anti-CD89 as a capture antibody and HRP-conjugated anti-IgA as a detection antibody. This method was used to measure serum levels of sCD89-IgA complexes in IgAN patients without immunosuppressant history and healthy subjects. Correlations between serum levels of sCD89-IgA complexes and disease severity were analyzed. Results: Serum sCD89-IgA complexes increased with age (P < 0.001). IgAN patients had higher sCD89-IgA complex levels compared with age- and gender-matched normal healthy individuals (P < 0.001). IgAN patients had higher sCD89-IgA complex levels compared with age- and gender-matched normal healthy individuals (P < 0.001). IgAN patients had higher sCD89-IgA complex levels compared with age- and gender-matched normal healthy individuals (. Conclusions: Serum sCD89-IgA complexes can guide diagnosis of IgAN in patients without immunosuppressant history, but provide limited help in clinicopathologic prediction.

6.
Urology ; 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32006548

RESUMO

OBJECTIVE: To illustrate our refinement technique for robotic intracorporeal orthotopic Hautmann neobladder with adherence to open surgical principles and evaluate perioperative and functional outcomes. PATIENTS AND METHODS: Robot-assisted radical cystectomy with intracorporeal Hautmann orthotopic neobladder was performed by the same surgeon in 40 patients with bladder cancer from November 2017 to March 2019. Baseline demographics, pathologic data, 90-day complications, and functional outcomes at both 6 and 12 months were evaluated with questionnaire and urodynamic analysis. RESULTS: Median follow-up was 14 months (range 4-20). Median operative time was 320 (230-500) minutes, and the estimated blood loss was 300 (100-2000) mL. No conversion to the open technique was reported. The overall 90-day complication rate was 45%, and the high-grade complication rate was only 10%. The daytime satisfactory continence rate was 90% at both 6 months (30 patients) and 12 months (20 patients), while the night-time satisfactory continence rate was 76.7% and 80.0% at 6 months and 12 months, respectively. One patient underwent clean intermittent catheterization. The cohort had minimal postvoid residual volume, normal compliance, and a mean capacity of 328.7 cm3 (range 170-500) at 6 months postoperatively. CONCLUSION: Our preliminary data indicate that robotic intracorporeal Hautmann neobladder configuration is a feasible surgical technique and can achieve a low pressure and sufficient capacity for satisfactory early voiding patterns. Refinement of the stepwise process can effectively decrease the time of the operation. Long-term functional and oncological outcomes remain to be evaluated with longer follow-up and more cases.

7.
BMC Gastroenterol ; 20(1): 7, 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31931737

RESUMO

BACKGROUND: The anti-immunological rejection therapy for small-for-size syndrome (SFSS) after live donor liver transplantation (LDLT) play a central role in keeping graft survival. The hepatocyte number and grafts function has undergone real-time changes with the proliferation and apoptosis of the grafts after reperfusion. Lacking an accurate and effective treatment regiments or indicators to guide the use of immunosuppressive drugs in SFS liver transplantation has made immunotherapy after SFS liver transplantation an urgent problem to be solved. Herein, we established small-for-size (SFS) and normal size liver transplantation model in rats to explore the effective indicators in guiding immunotherapy, to find an effective way for overcoming SFSS. METHODS: Lewis rats (donors) and BN rats (recipients) were used to mimic allograft liver transplantation and treated with tacrolimus. Local graft immune response was analyzed through haematoxylin and eosin and immunohistochemistry. Flow cytometry was used to assess the overall immune status of recipient. The pharmacokinetics mechanism of immunosuppressive drugs was explored through detecting CYP3A2 expression at mRNA level and protein levels. RESULTS: The results showed the local immune reaction of SFS grafts and systemic immune responses of recipient were significantly increased compared with those in normal size grafts and their recipient at four days after liver transplantation. Regression equation was used to regulate the tacrolimus dose which not only controlled tacrolimus serum concentration effectively but alleviated liver damage and improved survival rate. CONCLUSIONS: This study showed that AST level and tacrolimus serum concentrations are effective indicators in guiding immunotherapy. Regression equation (TD = - 0.494TC-0.0035AST + 260.487) based on AST and tacrolimus serum concentration can be used as a reference for adjustment of immunotherapy after SFS liver transplantation, which is applicable in clinical practice.

8.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31983458

RESUMO

OBJECTIVE: Gastric cancer (GC) has been become the second leading cause for cancer-associated death. This study aimed to investigate Orexin A levels and associated receptors in tumor tissues of GC patients. PATIENTS AND METHODS: Forty-six consecutive gastric cancer patients (GC, n=46) and 13 chronic atrophic gastritis patients (CAG, n=13) were recruited. Meanwhile, 18 health individuals visiting Medical Examination Department were involved as control (N group, n=18). ELISA was used to examine Orexin A concentration. Immunohistochemistry assay was used to examine OX1R and OX2R. HE staining was applied to evaluate inflammation. qRT-PCR was employed to detect OX1R, OX2R, prepro-Orexin mRNAs. Serum Helicobacter pylori (H. pylori) infection was measured. RESULTS: Orexin A expression in GC patients was significantly up-regulated compared to N group and CAG group (p<0.05). Orexin A expression was increased in CAG group compared to N group (p<0.05). Gastric cancer tissues exhibited significantly obvious inflammation compared to N group and CAG group (p<0.05). OX1R and OX2R expressions were significantly down-regulated in GC group compared to N group and CAG group (p<0.05). OX1R and OX2R were lower significantly in GC group compared to CAG group (p<0.05). Prepro-Orexin was significantly depleted in tumor tissues of GC group compared to N group and CAG group (p<0.05). Orexin A expression was un-associated with gender, age and differential grades (p>0.05). CAG and GC patients demonstrated higher H. pylori infection rates. CONCLUSION: Orexin A was associated with inflammation by interacting with OX1R/OX2R receptor and activating prepro-Orexin in tumor tissues of gastric cancer patients.

9.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 117999, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31935655

RESUMO

Bacillus anthracis spores have a unique biomarker of calcium dipicolinate (CaDPA). In this work, we reported a composite nanostructure for the optical sensing of DPA, with Eu (III)-doped metal-organic framework (MOF) as supporting lattice, a rhodamine-derived dye as sensing probe, respectively. By means of XRD, IR, TGA and photophysical analysis, this composite structure was carefully discussed. It was found that rhodamine absorption and emission were enhanced by DPA, while Eu emission was quenched by DPA. As a consequence, two sensing skills were observed from this composite structure, which are colorimetric sensing based on absorption spectra and ratiometric fluorescent sensing based on emission spectra. Linear sensing response was observed for both sensing channels with a warning signal at DPA concentration higher than 140 µM. Good selectivity was confirmed with a low LOD value of 0.52 µM. The sensing mechanism was revealed as the combination of emission turn-on effect triggered by DPA-released protons and emission turn-off effect originated from electron-transfer from EuBTC to DPA. This composite structure showed its advantage of naked eye detection and two sensing skills with linear response.

10.
Chem Biol Interact ; 317: 108943, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31926917

RESUMO

Epidemiological studies have shown that cigarette smoking is beneficial in ulcerative colitis and that nicotine may be responsible for this effect. However, the mechanism remains unclear. In a previous study, nicotine was found to induce autophagy in intestinal cells. Here, we evaluated the effect of nicotine-induced autophagy in a dextran sodium sulfate (DSS)-induced colitis mouse model. C57BL/6 adult male mice drank DSS water solution freely for seven consecutive days, and then tap water was administered. The effect of nicotine treatment was examined in the DSS model, including colon length, disease severity, histology of the colon tissue, and inflammation levels. Moreover, autophagy levels were detected by Western blot analysis (LC3II/LC3I, p62, and beclin-1). The levels of DSS-induced colitis were significantly decreased following nicotine treatment. The disease activity score, body weight, histologic damage scores, and the level of colonic inflammatory factors of nicotine-treated mice all decreased compared to those of the control mice. Additionally, nicotine enhanced the expression of LC3II/LC3I and beclin-1 but decreased the p62 protein level. Inhibiting autophagy by 3-MA attenuated the protective effects of nicotine on colitis. Additionally, both in vitro and in vivo experiments showed changes in AMPK-mTOR-P70S6K during this process. These results suggest that nicotine improved colitis by regulating autophagy and provided a protective effect against DSS-induced colitis.


Assuntos
Adenilato Quinase/metabolismo , Autofagia/efeitos dos fármacos , Colite/prevenção & controle , Nicotina/farmacologia , Serina-Treonina Quinases TOR/metabolismo , Adenina/análogos & derivados , Adenina/farmacologia , Adenilato Quinase/genética , Animais , Colite/induzido quimicamente , Sulfato de Dextrana/toxicidade , Regulação da Expressão Gênica/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Quinases S6 Ribossômicas 70-kDa/genética , Proteínas Quinases S6 Ribossômicas 70-kDa/metabolismo , Serina-Treonina Quinases TOR/genética
11.
Vasa ; 49(2): 141-146, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31920171

RESUMO

Background: A 4G/5G polymorphism in the promoter region of the plasminogen activator inhibitor type 1 (PAI-1) gene has been reported to enhance the plasma levels of PAI-1, which plays an important role in fibrinolysis disorders and venous thromboembolism, but a large number of studies have reported inconclusive results. Therefore, we performed a meta-analysis to analysis these associations. Materials and methods: We performed a publication search for articles published before April 2019 by using the electronic databases of web of Science, Embase, PubMed, CNKI, CBM and WanFang data with the following terms "PAI-1", "polymorphism", "Venous Thromboembolism". Two investigators independently extracted data and assessed study quality. Statistical analyses were undertaken using Stata 14.0. Results: A total of 27 studies, with 3135 patients and 5346 controls were included. Overall, the variant PAI-1 4G/4G and PAI-1 4G/5G was associated with venous thromboembolism risk, compared with the PAI-1 5G/5G allele in the populations included in the analysis. Stratified analysis revealed that PAI-1 4G/4G and PAI-1 4G/5G genotypes were associated with an increased VTE risk among Asia populations in all five genetic models. Conclusions: The PAI-1 4G/5G polymorphism may be a potential biomarker of VTE risk, particularly in Asia populations. Further larger studies with multi-ethnic populations are required to further assess the association between PAI-1 4G/4G polymorphisms and VTE risk.


Assuntos
Inibidor 1 de Ativador de Plasminogênio/genética , Tromboembolia Venosa , Predisposição Genética para Doença , Humanos , Plasminogênio , Polimorfismo Genético , Regiões Promotoras Genéticas , Tromboembolia Venosa/genética
12.
ACS Synth Biol ; 9(2): 343-355, 2020 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-31891494

RESUMO

The treatment of bladder cancer has recently shown minimal progress. Gene therapy mediated by CRISPR provides a new option for bladder cancer treatment. In this study, we developed a versatile liposome system to deliver the CRISPR-Cas13a gene circuits into bladder cancer cells. After in vitro studies and intravesical perfusion studies in mice, this system showed five advantages: (1) CRISPR-Cas13a, a transcriptional targeting and cleavage tool for gene expression editing, did not affect the stability of the cell genome; (2) the prepared liposome systems were targeted to hVEGFR2, which is always highly expressed in bladder cancer cells; (3) the CRISPR-Cas13a sequence was driven by an artificial tumor specific promoter to achieve further targeting; (4) a near-infrared photosensitizer released using near-infrared light was introduced to control the delivery system; and (5) the plasmids were constructed with three crRNA tandem sequences to achieve multiple targeting and wider therapeutic results. This tumor cell targeting lipid delivery system with near-infrared laser-controlled ability provided a versatile strategy for CRISPR-Cas13a based gene therapy of bladder cancer.

13.
Brain Imaging Behav ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31898093

RESUMO

Cerebral microbleeds (CMBs) in dialysis patients have recently attracted much attention, and the different locations of CMBs indicate different pathological processes. Previous studies on the relationship between CMBs and cognitive impairment (CI) in the general population and in dialysis patients have reported controversial results. A total of 180 chronic dialysis patients were enrolled in our study. Based on brain magnetic resonance imaging (MRI) analysis of CMBs, the patients were divided into 4 groups (without-CMBs group, strictly lobar group, strictly deep group, and mixed group). A wide range of cognitive tests was administered to evaluate cognitive function. The risk factors for CMBs were explored, and the correlation between CMB distribution and CI was investigated by regression analysis. The prevalence of CMBs was 32.8% in the total study population, 36.1% in the haemodialysis (HD) subgroup and 26.2% in the peritoneal dialysis (PD) PD subgroup. Sixteen subjects (8.9%) were classified as the lobar group, 12 subjects (6.7%) as the mixed group, and 31 subjects (17.2%) as the deep group. A significant association was shown between deep CMBs and impaired cognitive function, involving overall cognitive function, memory, language ability and executive function. Deep CMBs were significantly associated with dialysis vintage, mean arterial pressure (MAP) and lacunar infarcts number, while deep CMBs showed no correlation with dialysis modality and current heparin medication. Deep CMBs are closely associated with global and specific CI in dialysis patients. Blood pressure control may prevent deep CMBs and their associated CI.

14.
J Cell Biochem ; 121(3): 2258-2267, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31693222

RESUMO

Lung cancer is famous as an aggressive malignant tumor and is the main cause of cancer-associated mortality globally. Tumor angiogenesis is a vital part in cancer, which influences cell proliferation and metastasis. Increasing studies have claimed that long noncoding RNAs (lncRNAs) were involved in the progression of several cancers. Based on previous studies, this study focused on the role and mechanism of lncRNA MCM3AP antisense RNA 1 (MCM3AP-AS1) in lung cancer. At first, MCM3AP-AS1 expression was found to be elevated in lung cancer cells. Depletion of MCM3AP-AS1 repressed cell proliferation, migration, and angiogenesis in lung cancer cells. YY1 was confirmed to mediate MCM3AP-AS1 transcription in lung cancer cells. Moreover, the molecular mechanism investigation revealed that MCM3AP-AS1 could sponge miR-340-5p and elevate KPNA4 expression. On the basis of rescue assays, we identified that the overexpression of KPNA4 partly counteracted the suppressed effect of MCM3AP-AS1 knockdown on angiogenesis and progression in lung cancer cells. Conclusively, the YY1-mediated overexpression of MCM3AP-AS1 accelerated angiogenesis and progression in lung cancer by targeting miR-340-5p/KPNA4 axis, which highlighted the possibility of MCM3AP-AS1 as a promising therapeutic target for lung cancer.

15.
Clin Exp Pharmacol Physiol ; 47(3): 439-448, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31587336

RESUMO

Dysregulation of long non-coding RNA papillary thyroid carcinoma susceptibility candidate 3 (lncRNA PTCSC3) has been found to correlate with various types of cancers. Quantitative RT-PCR showed a down-regulation of PTCSC3 in cervical cancer tissues compared with normal cervical tissues. The present study aimed to investigate the role of lncRNA PTCSC3 in cervical cancer and the underlying mechanisms. PTCSC3 was overexpressed in cervical cancer cell lines C-33A and Hela by transfection with pcDNA3.1-lncRNA PTCSC3 expressing plasmid. Overexpression of lncRNA PTCSC3 inhibited cell proliferation, induced cell cycle arrest, and suppressed cell invasion and migration using CCK8 assay, flow cytometry, Transwell assay and wound healing examination, respectively. Western blotting analysis showed that PTCSC3 overexpression decreased the expression of cyclinD1, matrix metalloproteinases 9 (MMP9), N-cadherin and ß-catenin and increased E-cadherin expression. Further, PTCSC3 negatively regulated miR-574-5p expression and dual-luciferase assay verified the binding activity between miR-574-5p and lncRNA PTCSC3. Enforced up-regulation of miR-574-5p abolished the inhibitory effect of lncRNA PTCSC3 on cervical cancer cell proliferation, invasiveness and mobility. Taken together, lncRNA PTCSC3 inhibited cell growth and metastasis via sponging miR-574-5p in cervical cancer. Therefore, we demonstrate the tumour-suppressive function of lncRNA PTCSC3 in cervical cancer and suggest that PTCSC3 is a potential therapeutic target for cervical cancer.

16.
Invest New Drugs ; 38(1): 148-159, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31399906

RESUMO

Background The 5-year survival rate for extensive-disease small-cell lung carcinoma (ED-SCLC) is only 1%. Recently, apatinib exerted promising effects on cancer patients after failure of first-line chemotherapy. Methods This study enrolled 24 ED-SCLC patients to study the efficacy and toxicity of apatinib in combination with chemotherapy and maintenance therapy. The primary endpoints were overall survival (OS) and progression-free survival (PFS). The secondary endpoints included toxicity and safety. Apatinib was given 250 mg/day during the chemotherapy interval, and as maintenance therapy after 4-6 cycles until the patient progressed, died, or was intolerant to drug toxicity. The study further evaluated the cytotoxicity, cell-cycle arrest and apoptotic induction of apatinib in A549 and H446 cells. Results There was no difference in short-term efficacy between combined and chemotherapy groups. Long-term efficacy showed that the median PFS was 7.8 months and 4.9 months in combination and chemotherapy groups, respectively [p = 0.002, HR(95%CI): 0.18(0.06-0.60)]. The median OS was 12.1 months and 8.2 months in combination and chemotherapy groups, respectively [p = 0.023, HR(95%CI): 0.38 (0.16-0.90)]. Multivariate Cox regression analysis showed that apatinib combined with chemotherapy was an independent prognostic factor for OS and PFS. The ECOG score was an independent prognostic factor affecting OS. In vitro analysis showed that apatinib inhibited cell proliferation and caused cell-cycle arrest and apoptosis. Conclusion Apatinib combination/maintenance therapy showed promising efficacy and safety to extend OS/PFS in ED-SCLC and will be a potent therapeutic option in future practice. Although the scale of this study is small, further research on large sample sizes is needed.

17.
J Cosmet Dermatol ; 19(1): 211-217, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31461211

RESUMO

BACKGROUND: The repair and reconstruction of the upper lip defects should focus on the important anatomical landmarks of the upper lip. In this investigation, double V-Y advancement flaps were used to simultaneously repair the cutaneous and mucosal defects of the upper lip. It was especially suitable for young patients with tight skin and high-risk scar hyperplasia in the donor sites. OBJECTIVE: The objective was to examine the surgical outcomes following the simultaneous repairation of upper lip mucocutaneous defects using double V-Y advancement flaps. METHODS: A retrospective review of all patients with defects near the vermilion border who underwent double V-Y flaps repair from July 2014 to November 2018 was performed. Transverse V-Y advancement flaps were used to repair the cutaneous defects and longitudinal V-Y advancement flaps to repair the mucosal defects. RESULTS: Fifteen patients (six males, nine females) were retrospectively reviewed. Defects spanning the vermilion border ranged from 0.8 × 0.5 to 2.5 × 1.5 cm2 . Follow-up was for 3 months or longer. There were no perioperative complications or visible postoperative scars, and major anatomic landmarks were preserved and reconstructed. All patients were satisfied with the aesthetic outcome. CONCLUSION: Double V-Y advancement flaps are suitable for the repair of superficial mucocutaneous defects smaller than 50% of the lateral upper lip, especially for younger patients with tight skin.

18.
Int Urol Nephrol ; 52(2): 337-342, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31820359

RESUMO

PURPOSE: The association of type 2 diabetes with proteinuria remission and renal function decline in patients with idiopathic membranous nephropathy (IMN) remains elusive. This study was designed to assess such association. METHODS: In this retrospective cohort study, we included 656 IMN patients treated with immunosuppressants or plus corticosteroids, of whom 72 were diagnosed as type 2 diabetes prior to or at diagnosis of IMN. Data on age, sex, body mass index, presence of hypertension and diabetes, laboratory tests, and therapeutic regimens were retrospectively retrieved from medical record. Cox regression was used to analyze risks of failure to achieve remission, relapse, and developing a ≥ 30% decline in estimated glomerular filtration rate (eGFR) or end-stage renal disease (ESRD) associated with baseline diabetes. RESULTS: The patients were followed for 36.6 (IQR 17.5-59.0) months, of whom 451 reached complete remission, 92 achieved partial remission, and 61 developed a ≥ 30% eGFR decline or ESRD. IMN relapse occurred in 30.6% of the 543 remitted patients. Baseline diabetes was associated with failure to achieve complete remission (HR 0.61, 95% CI 0.43-0.86, P = 0.005) in patients with IMN, independently of age, sex, hypertension, baseline serum albumin, urine protein levels, and eGFR, and therapeutic regimens. However, we failed to identify independent association between baseline diabetes and failure to achieve total remission (HR 0.85, 95% CI 0.63-1.1, P = 0.29), IMN relapse (OR 0.92, 95% CI 0.49-1.7, P = 0.80), or ≥ 30% decline in eGFR or ESRD (HR 1.4, 95% CI 0.78-2.7, P = 0.24) in patients with IMN. CONCLUSIONS: Baseline diabetes may be independently associated with failure to achieve complete remission, but not with IMN relapse and renal function decline in IMN patients.

19.
Rehabil Nurs ; 45(2): 88-96, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-29916900

RESUMO

PURPOSE: This study aims to investigate the willingness to pay (WTP) for a home-based rehabilitation service and explore the influencing factors of WTP among older adults in Shanghai, China. DESIGN: A cross-sectional design was used. METHODS: A questionnaire survey based on the contingent valuation method was conducted by face-to-face survey over 3 months. FINDINGS: Only 242 (44%) participants were willing to pay for a home-based rehabilitation service. The median amount they were willing to pay was RMB 8 (US$1.15) per visit. Older adults who had higher monthly income, had at least one partner who worked, and had medical insurance were willing to pay more for the service. CONCLUSIONS: Older adults showed low WTP for a home-based rehabilitation service. Economic status and health condition are the significant influencing factors of WTP. CLINICAL RELEVANCE: Studies on recipients' precise needs and ability to pay are required before home-based services are implemented.

20.
ACS Appl Mater Interfaces ; 12(2): 2733-2742, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31856566

RESUMO

Halogenation, for example, fluorination and chlorination, is an effective strategy to regulate the performance of organic photovoltaic materials. Although fluorination has been widely applied to polymer acceptors, systematic studies on the comparison of nonhalogenated, fluorinated, and chlorinated polymer acceptors have been a blank to now. Herein, a B ← N embedded electron-deficient unit (A), namely, BNIDT was copolymerized with three electron-rich units (D), that is, benzodithiophene (BDT), fluorinated BDT, and chlorinated BDT to obtain three D-A polymers of BN-BDT, BN-BDT-F, and BN-BDT-Cl, respectively. The three polymers exhibit similar LUMOs of ca. -3.77 eV, whereas the HOMOs are remarkably decreased from BN-BDT (-5.46 eV) to BN-BDT-F (-5.71 eV) and further slightly lowered to BN-BDT-Cl (-5.74 eV). All-polymer solar cells (all-PSCs) were fabricated using PBDB-T as the donor and the three B ← N-based polymers as the acceptors. The efficiencies of all-PSCs were significantly promoted from nonhalogenated BN-BDT (1.60%) to fluorinated BN-BDT-F (3.71%) and further elevated to chlorinated BN-BDT-Cl (4.23%). Device characterizations revealed that halogenation on the polymer acceptors leads to enhanced hole-transfer driving forces and better donor/acceptor miscibility, for example, smaller domain sizes and root-mean-square roughness (rms) values, which further gives rise to higher and more balanced hole/electron mobilities and efficient physical processes, for example, efficient exciton dissociation and collection and weaker recombination losses in halogenated devices. This work demonstrates that the photovoltaic performance of nonhalogenated polymer acceptors can be remarkably boosted by fluorination and further enhanced by chlorination. This is the first systematic study on the halogenated polymer acceptors by comprehensively comparing nonhalogenated, fluorinated, and chlorinated ones.

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