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1.
J Hazard Mater ; 446: 130652, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36603420

RESUMO

Per- and polyfluoroalkyl substances (PFASs) present adverse effects for human health, which result in strong needs for reliable tools monitoring personal exposure to PFASs. This study manufactured two wristbands of high density polyethylene (HDPE) and thermoplastic polyurethane (TPU), and used the wristbands to monitor PFASs personal exposure. The analytical method was developed to measure 32 PFASs in the paired HDPE and TPU wristbands worn by 60 postgraduates. Twenty-nine of 32 PFASs were detected and hexafluoropropylene oxide dimer acid (HFPO-DA) was predominant individual PFASs with median concentrations of 337 and 554 pg/g for HDPE and TPU wristbands respectively. The gender and grade of students had moderate effects on PFASs distribution in the wristbands. Higher PFASs levels were determined in the two wristbands worn by the male students compared to the females, and the greatest PFASs concentration was observed in the wristbands worn by the first-year postgraduates, follow by second- and third-year postgraduates. Additionally, significant correlations between paired HDPE and TPU wristbands were observed for perfluorobutanoic acid (PFBA), perfluorohexane sulfonic acid (PFHxS), perfluoroheptane sulfonic acid (PFHpS), perfluorooctane sulfonic acid (PFOS), and HFPO-DA. These results suggest that HDPE and TPU wristbands can be used as effective tools for monitoring personal PFAS exposure.


Assuntos
Ácidos Alcanossulfônicos , Fluorcarbonetos , Feminino , Humanos , Masculino , Polietileno , Poliuretanos , Fluorcarbonetos/análise , Estudantes , Monitoramento Ambiental
2.
Psychiatry Res ; 321: 115066, 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36716552

RESUMO

Acute insomnia is common and a substantial proportion of people with acute insomnia (i.e. 3 days to 3 months) transit into chronic insomnia (i.e. 3 months or longer). Therefore, early intervention for acute insomnia is vital to prevent chronicity. Previous trials with small sample sizes have shown that brief versions of both individual and group-based face-to-face cognitive behavioral therapy for insomnia (CBT-I) can improve insomnia symptoms among those with acute insomnia. However, it is unknown whether one-week internet-delivered cognitive behavioral therapy for insomnia (CBT-I) is effective in treating acute insomnia. This was a randomized controlled trial and 192 participants were randomly assigned to the CBT-I group (n = 95) or control group (n = 97). The primary outcome was the incidence of chronic insomnia, determined via a structured diagnostic questionnaire for insomnia disorders according to the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition. Secondary outcomes were Insomnia Severity Index (ISI), Dysfunctional Beliefs and Attitudes about Sleep (DBAS), Epworth Sleepiness Scale (ESS), Pre-sleep Arousal Scale (PSAS), Ford Insomnia Response to Stress Test (FIRST), Sleep Hygiene and Practices Scale (SHPS), Hospital Anxiety and Depression Scale (HADS), and Short-Form 12-Item Health Survey version 2 (SF-12v2). At week 12, the incidence of chronic insomnia was significantly lower in the CBT-I group compared with control group (33.3% [27/81] vs. 65.8% [52/79]). Participants in the CBT-I group achieved significantly more improvements in ISI, ESS, PSAS, FIRST, SHPS, HADS-Depression, and the mental component summary and physical component summary of SF-12v2 than control group, but not DBAS and HADS-Anxiety. This one-week internet-delivered CBT-I program is an effective tool to prevent the chronicity of acute insomnia.

3.
Ther Adv Hematol ; 14: 20406207221149245, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36654739

RESUMO

Hodgkin lymphoma is a special type of lymphoma in which tumor cells frequently undergo multiple genetic lesions that are associated with accompanying pathway abnormalities. These pathway abnormalities are dominated by active signaling pathways, such as the JAK-STAT (Janus kinase-signal transducer and activator of transcription) pathway and the NFκB (nuclear factor kappa-B) pathway, which usually result in hyperactive survival signaling. Targeted therapies often play an important role in hematologic malignancies, such as CAR-T therapy (chimeric antigen receptor T-cell immunotherapy) targeting CD19 and CD22 in diffuse large B-cell lymphoma, while in Hodgkin lymphoma, the main targets of targeted therapies are CD30 molecules and PD1 molecules. Drugs targeting other molecules are also under investigation. This review summarizes the actionable genetic lesions, current treatment options, clinical trials for Hodgkin lymphoma and the potential value of those genetic lesions in clinical applications.

4.
Science ; 379(6629): 288-294, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36656941

RESUMO

High-quality perovskite light harvesters and robust organic hole extraction layers are essential for achieving high-performing perovskite solar cells (PSCs). We introduce a phosphonic acid-functionalized fullerene derivative in mixed-cation perovskites as a grain boundary modulator to consolidate the crystal structure, which enhances the tolerance of the film against illumination, heat, and moisture. We also developed a redox-active radical polymer, poly(oxoammonium salt), that can effectively p-dope the hole-transporting material by hole injection and that also mitigates lithium ion diffusion. Power conversion efficiencies of 23.5% for 1-square-centimeter mixed-cation-anion PSCs and 21.4% for 17.1-square-centimeter minimodules were achieved. The PSCs retained 95.5% of their initial efficiencies after 3265 hours at maximum power point tracking under continuous 1-sun illumination at 70° ± 5°C.

5.
Anal Bioanal Chem ; 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36680590

RESUMO

Hexavalent chromium is a highly toxic substance, which will pose a serious threat to human life and health and the entire ecosystem. Therefore, it is crucial to establish a simple and rapid detection method for hexavalent chromium. In this work, we fabricated bovine serum albumin-stabilized silver nanocluster (BSA-Ag13 NC) which exhibited photoresponsive oxidase-like activity, catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to the blue oxidized state TMB (oxTMB) in a short time. Interestingly, 8-hydroxyquinoline (8-HQ) can significantly inhibit the color reaction of TMB oxidation while Cr(VI) can interact specifically with 8-HQ to restore this chromogenic reaction. Based on the above facts, a colorimetric sensing system for detecting Cr(VI) was developed. The sensing system shows a wide linear range, and good selectivity, with a low detection limit of 2.32 nM. Moreover, this sensing system could be successfully applied to the detection of Cr(VI) in lake water, tap water, and sewage with satisfactory results.

6.
Science ; 379(6630): 399-403, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36701445

RESUMO

Daily temperature variations induce phase transitions and lattice strains in halide perovskites, challenging their stability in solar cells. We stabilized the perovskite black phase and improved solar cell performance using the ordered dipolar structure of ß-poly(1,1-difluoroethylene) to control perovskite film crystallization and energy alignment. We demonstrated p-i-n perovskite solar cells with a record power conversion efficiency of 24.6% over 18 square millimeters and 23.1% over 1 square centimeter, which retained 96 and 88% of the efficiency after 1000 hours of 1-sun maximum power point tracking at 25° and 75°C, respectively. Devices under rapid thermal cycling between -60° and +80°C showed no sign of fatigue, demonstrating the impact of the ordered dipolar structure on the operational stability of perovskite solar cells.

7.
Int J Pharm ; : 122638, 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36702386

RESUMO

Recently, there has been an increase in the use of numerical simulation technology in pharmaceutical preparation processes. Numerical simulation can contribute to a better understanding of processes, reduce experimental costs, optimize preparation processes, and improve product quality. The intermediate material of most dosage forms is powder or granules, especially in the case of solid preparations. The macroscopic behavior of particle materials is controlled by the interactions of individual particles with each other and surrounding fluids. Therefore, it is very important to analyze and control the microscopic details of the preparation process for solid preparations. Since tablets are one of the most widely used oral solid preparations, and the preparation process is relatively complex and involves numerous units of operation, it is especially important to analyze and control the tablet production process. The present paper discusses recent advances in numerical simulation technology for the preparation of tablets, including drying, mixing, granulation, tableting, and coating. It covers computational fluid dynamics (CFD), discrete element method (DEM), population balance model (PBM), finite element method (FEM), Lattice-Boltzmann model (LBM), and Monte Carlo model (MC). The application and deficiencies of these models in tablet preparation unit operations are discussed. Furthermore, the paper provides a systematic reference for the control and analysis of the tablet preparation process and provides insight into the future direction of numerical simulation technology in the pharmaceutical industry.

8.
J Environ Manage ; 331: 117290, 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36642050

RESUMO

Waste-activated sludge (WAS) is regarded as a source of hazardous waste pollution from sewage treatment plants. To efficiently deal with WAS, vortex cavitation circulating fluidised grinding technology (VCCFGT) was proposed as a novel circulating fluidisation technology (CFT) to disintegrate WAS. To be specific, we investigated the effects of disintegration duration, pressure, and filling ratio of mill balls on sludge disintegration. The results of chemical and physical evaluation showed that the values of soluble chemical oxygen demand (SCOD), disintegration degree (DDSCOD), DNA, protein, carbohydrate, and NH4+-N increased with the increase in the filling ratio of the mill balls. Under a pressure and filling ratio of 0.30 Mpa and 1.6%, respectively, the maximum effect was achieved after 60 min of treatment. Compared to those in the treatment without mill balls, the values of SCOD, DDSCOD, DNA, protein, carbohydrate, and NH4+-N in the treatment using mill balls increased by 218, 229, 230, 177, 371, and 190%, respectively. As a result of this technology, the temperature of the sludge dramatically increased, rising approximately 42.9 °C. Compared to that of the raw sludge, the sludge particle size after treatment was reduced by 83.25% at most, and the morphology of the sludge comprised smaller flocs. Compared to that of the ball-milling method, the mill balls filling ratio of VCCFGT reduced by 93.60-98.12%. Compared to that of sludge disintegration by the vortex cavitation method, VCCFGT indicating good disintegration degree (increased by 229%) and economic feasibility. VCCFGT has good application prospects for sludge disintegration. The main mechanisms of sludge disintegration and organic release include centrifugal force, grinding, shear force, cavitation, and cyclic fatigue effects, among which grinding plays a leading role. This study concluded that CFT can effectively disintegrate sludge flocs and disrupt bacterial cell walls.

9.
Biomed Pharmacother ; 159: 114250, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36652733

RESUMO

Mucosal-associated invariant T (MAIT) cells are resident T cells that express semi-invariant TCR chains and are restricted by monomorphic major histocompatibility complex (MHC) class I-related molecules (MR1). MAIT cells can be activated by microbial-specific metabolites (MR1-dependent mode) or cytokines (MR1-independent mode). Activated MAIT cells produce chemokines, cytotoxic molecules (granzyme B and perforin), and proinflammatory cytokines (IFN-γ, TNF-α, and IL-17), to clear pathogens and target infected cells involved in the pro-inflammatory, migratory, and cytolytic properties of MAIT cells. MAIT cells produce pro-inflammatory cytokines in the target organs of autoimmune diseases and contribute to the development and progression of autoimmune diseases. This article reviews the biological characteristics, activation mechanism, dynamic migration, and dual functions of MAIT cells, and focuses on the mechanism and potential application of MAIT cells in the early diagnosis, disease activity monitoring, and therapeutic targets of autoimmune diseases, to lay a foundation for future research.

10.
JAMA Netw Open ; 6(1): e2252553, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36692877

RESUMO

Importance: Tertiary lymphoid structures (TLSs) are associated with a favorable prognosis and improved response to cancer immunotherapy. The current approach for evaluation of TLSs is limited by interobserver variability and high complexity and cost of specialized imaging techniques. Objective: To develop a machine learning model for automated and quantitative evaluation of TLSs based on routine histopathology images. Design, Setting, and Participants: In this multicenter, international diagnostic/prognostic study, an interpretable machine learning model was developed and validated for automated detection, enumeration, and classification of TLSs in hematoxylin-eosin-stained images. A quantitative scoring system for TLSs was proposed, and its association with survival was investigated in patients with 1 of 6 types of gastrointestinal cancers. Data analysis was performed between June 2021 and March 2022. Main Outcomes and Measures: The diagnostic accuracy for classification of TLSs into 3 maturation states and the association of TLS score with survival were investigated. Results: A total of 1924 patients with gastrointestinal cancer from 7 independent cohorts (median [IQR] age ranging from 57 [49-64] years to 68 [58-77] years; proportion by sex ranging from 214 of 409 patients who were male [52.3%] to 134 of 155 patients who were male [86.5%]). The machine learning model achieved high accuracies for detecting and classifying TLSs into 3 states (TLS1: 97.7%; 95% CI, 96.4%-99.0%; TLS2: 96.3%; 95% CI, 94.6%-98.0%; TLS3: 95.7%; 95% CI, 93.9%-97.5%). TLSs were detected in 62 of 155 esophageal cancers (40.0%) and up to 267 of 353 gastric cancers (75.6%). Across 6 cancer types, patients were stratified into 3 risk groups (higher and lower TLS score and no TLS) and survival outcomes compared between groups: higher vs lower TLS score (hazard ratio [HR]; 0.27; 95% CI, 0.18-0.41; P < .001) and lower TLS score vs no TLSs (HR, 0.65; 95% CI, 0.56-0.76; P < .001). TLS score remained an independent prognostic factor associated with survival after adjusting for clinicopathologic variables and tumor-infiltrating lymphocytes (eg, for colon cancer: HR, 0.11; 95% CI, 0.02-0.47; P = .003). Conclusions and Relevance: In this study, an interpretable machine learning model was developed that may allow automated and accurate detection of TLSs on routine tissue slide. This model is complementary to the cancer staging system for risk stratification in gastrointestinal cancers.


Assuntos
Neoplasias Gástricas , Estruturas Linfoides Terciárias , Humanos , Masculino , Pessoa de Meia-Idade , Feminino , Estruturas Linfoides Terciárias/patologia , Prognóstico , Estadiamento de Neoplasias , Linfócitos do Interstício Tumoral/patologia , Neoplasias Gástricas/patologia
11.
Burns Trauma ; 11: tkac052, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36694861

RESUMO

Background: Excessive scarring and fibrosis are the most severe and common complications of burn injury. Prolonged exposure to high levels of glucocorticoids detrimentally impacts on skin, leading to skin thinning and impaired wound healing. Skin can generate active glucocorticoids locally through expression and activity of the 11ß-hydroxysteroid dehydrogenase type 1 enzyme (11ß-HSD1). We hypothesised that burn injury would induce 11ß-HSD1 expression and local glucocorticoid metabolism, which would have important impacts on wound healing, fibrosis and scarring. We additionally proposed that pharmacological manipulation of this system could improve aspects of post-burn scarring. Methods: Skin 11ß-HSD1 expression in burns patients and mice was examined. The impacts of 11ß-HSD1 mediating glucocorticoid metabolism on burn wound healing, scar formation and scar elasticity and quality were additionally examined using a murine 11ß-HSD1 genetic knockout model. Slow-release scaffolds containing therapeutic agents, including active and inactive glucocorticoids, were developed and pre-clinically tested in mice with burn injury. Results: We demonstrate that 11ß-HSD1 expression levels increased substantially in both human and mouse skin after burn injury. 11ß-HSD1 knockout mice experienced faster wound healing than wild type mice but the healed wounds manifested significantly more collagen deposition, tensile strength and stiffness, features characteristic of excessive scarring. Application of slow-release prednisone, an inactive glucocorticoid, slowed the initial rate of wound closure but significantly reduced post-burn scarring via reductions in inflammation, myofibroblast generation, collagen production and scar stiffness. Conclusions: Skin 11ß-HSD1 expression is a key regulator of wound healing and scarring after burn injury. Application of an inactive glucocorticoid capable of activation by local 11ß-HSD1 in skin slows the initial rate of wound closure but significantlyimproves scar characteristics post burn injury.

12.
Int J Nanomedicine ; 18: 225-241, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36660337

RESUMO

Background: Gallium (III) metal-organic complexes have been shown to have the ability to inhibit tumor growth, but the poor water solubility of many of the complexes precludes further application. The use of materials with high biocompatibility as drug delivery carriers for metal-organic complexes to enhance the bioavailability of the drug is a feasible approach. Methods: Here, we modified the ligands of gallium 8-hydroxyquinolinate complex with good clinical anticancer activity by replacing the 8-hydroxyquinoline ligands with 5-bromo-8-hydroxyquinoline (HBrQ), and the resulting Ga(III) + HBrQ complex had poor water solubility. Two biocompatible materials, bovine serum albumin (BSA) and graphene oxide (GO), were used to synthesize the corresponding Ga(III) + HBrQ complex nanoparticles (NPs) BSA/Ga/HBrQ NPs and GO/Ga/HBrQ NPs in different ways to enhance the drug delivery of the metal complex. Results: Both of BSA/Ga/HBrQ NPs and GO/Ga/HBrQ NPs can maintain stable existence in different solution states. In vitro cytotoxicity test showed that two nanomedicines had excellent anti-proliferation effect on HCT116 cells, which shown higher level of intracellular ROS and apoptosis ratio than that of cisplatin and oxaliplatin. In addition, the superior emissive properties of BSA/Ga/HBrQ NPs and GO/Ga/HBrQ NPs allow their use for in vivo imaging showing highly effective therapy in HCT116 tumor-bearing mouse models. Conclusion: The use of biocompatible materials for the preparation of NPs against poorly biocompatible metal-organic complexes to construct drug delivery systems is a promising strategy that can further improve drug delivery and therapeutic efficacy.


Assuntos
Antineoplásicos , Portadores de Fármacos , Gálio , Grafite , Nanopartículas Metálicas , Oxiquinolina , Animais , Humanos , Camundongos , Materiais Biocompatíveis , Linhagem Celular Tumoral , Portadores de Fármacos/síntese química , Gálio/química , Grafite/química , Células HCT116 , Nanopartículas Metálicas/análise , Nanopartículas/análise , Oxiquinolina/química , Tamanho da Partícula , Soroalbumina Bovina/farmacologia , Água , Antineoplásicos/síntese química , Antineoplásicos/química
13.
Anat Rec (Hoboken) ; 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36655864

RESUMO

The effectiveness and safety of electroacupuncture (EA) for constipation have been confirmed by numerous clinical studies and experiments, and there are also studies on the efficacy of EA for Parkinson's disease (PD) motor symptoms. However, there are few researches on EA for PD constipation. Autophagy is thought to be involved in the mechanistic process of EA in the central nervous system (CNS) intervention in Parkinson's pathology. However, whether it has the same effect on the enteric nervous system (ENS) has not been elucidated. Therefore, we investigated whether EA at Tianshu (ST25) acupoint promotes the clearance of α-Syn and damaged mitochondria aggregated in the ENS in a model of rotenone-induced PD constipation. This study evaluated constipation symptoms by stool characteristics, excretion volume, and water content, and the expression levels of colonic ATG5, LC3II, and Parkin were detected by Western Blot (WB) and Real-Time Quantitative PCR (RT-qPCR). The relationship between the location of α-Syn and Parkin in the colonic ENS was observed by immunofluorescence (IF). The results showed that EA intervention significantly relieved the symptoms of rotenone-induced constipation in PD rats, reversed the rotenone-induced down-regulation of colonic ATG5, LC3II, and Parkin expression, and the positional relationship between colonic α-Syn and Parkin proved to be highly correlated. It is suggested that EA might be helpful in treating PD constipation by modulating Parkin-induced mitochondrial autophagy.

14.
J Org Chem ; 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631394

RESUMO

The treatment of 2-alkenylanilines with phenyliodine(III) diacetate (PIDA) and LiBr or KI in HFIP was found to afford the corresponding 3-haloindoles via cascade oxidative cyclization/halogenation encompassing oxidative C-N/C-X (X = Br, I) bond formations. A plausible mechanism involving the in situ formation of the reactive AcO-X (X = Br, I) from the reaction of PIDA and LiBr/KI was postulated.

15.
Soft Matter ; 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36633123

RESUMO

Water-repellent interfaces with high performance have emerged as an indispensable platform for developing advanced materials and devices. Inspired by the pitcher plant, slippery liquid-infused porous surfaces (SLIPSs) with reliable hydrophobicity have proven to possess great potential for various applications in droplet and bubble manipulation, droplet energy harvesting, condensation, fog collection, anti-icing, and anti-biofouling due to their excellent properties such as persistent surface hydrophobicity, molecular smoothness, and fluidity. This review aims to introduce the development history of interaction between SLIPSs and fluids as well as the design principles, preparation methods, and various applications of some of the more typical SLIPSs. The fluid manipulation strategies of the slippery surfaces have been proposed including the wettability pattern, oriented micro-structure, and geometric gradient. At last, the application prospects of SLIPSs in various fields and the challenges in the design and fabrication of slippery surfaces are analyzed. We envision that this review can provide an overview of the fluid manipulating processes on slippery surfaces for researchers in both academic and industrial fields.

16.
Burns ; 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36642662

RESUMO

Ablative fractional resurfacing is clinically an efficient treatment for burn scar management. The aim of this pilot study was to investigate the poorly understood mechanisms underlying ablative fractional CO2 laser (AFL-CO2) therapy in relation to biomarkers S100 and 11ß-hydroxysteroid dehydrogenase type 1 (11ß-HSD1). S100 stains for Langerhans cells and neuronal cells, potentially representing the pruritus experienced. 11ß-HSD1 catalyses the interconversion of cortisol and cortisone in cells, promoting tissue remodelling. Immunohistochemical analysis of S100 and 11ß-HSD1 protein expression in the dermis and epidermis of the skin was performed on normal skin, before and after AFL-CO2 therapy. Data assessing outcome parameters was collected concurrently with the skin biopsies. 13 patients were treated with AFL-CO2 therapy. Langerhans cells decreased by 39% after 2nd treatment. Neuronal cells were overexpressed before treatment in the scar tissue by 91% but levels returned to that resembling normal skin. 11ß-HSD1 expression in keratinocytes was significantly higher after laser treatment compared to before in scar tissue (p <0.01). No clear correlation was found in dermal fibroblast numbers throughout the treatment course. Whilst the role of the explored mechanisms and their association with clinical outcomes cannot conclusively be stated, this pilot study demonstrates promising trends that encourages investigation into this relationship.

17.
iScience ; 26(1): 105861, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36624845

RESUMO

Epithelial ovarian cancer (EOC) can originate from either fallopian tube epithelial (FTE) or ovarian surface epithelial (OSE) cells, but with different latencies and disease outcomes. To address the basis of these differences, we performed single cell RNA-sequencing of mouse cells isolated from the distal half of fallopian tube (FT) and surface layer of ovary. We find at the molecular level, FTE secretory stem/progenitor cells and OSE cells resemble mammary luminal progenitors and basal cells, respectively. An FT stromal subpopulation, enriched with Pdgfra + and Esr1 + cells, expresses multiple secreted factor (e.g., IGF1) and Hedgehog pathway genes and may serve as a niche for FTE cells. In contrast, Lgr5 + OSE cells express similar genes largely by themselves, raising a possibility that they serve as their own niche. The differences in intrinsic expression programs and niche organizations of FTE and OSE cells may contribute to their different courses toward the development of EOCs.

19.
Sci Rep ; 13(1): 1607, 2023 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-36709337

RESUMO

Since the damage of the bridge structure may cause great disasters, it is necessary to monitor its health status, especially the bridge bearing, the important connecting component of the bridge's upper and lower structures. Nowadays, manual inspection is the main method to get the information of the bridge bearings' work status. However, occasional damage of bridge bearing may not be detected in time, and sometime the installation position of the bearing makes the manual inspection on bridge bearing difficult and even impossible. Therefore, in order to know the work status of the bridge bearings timely, an intelligent remote monitoring system for the bridge bearing is developed. A 32-channel real-time acquisition system is designed by using an AD7768-1 analog-to-digital converter and Xilinx Spartan-6 Field Programmable Gate Array for interface stress continuously acquired in the bridge bearing. To assure the good linearity and low noise performance of the monitoring system, the data acquisition card is meticulously designed to reduce noise from both hardware and software and realize high-precision acquisition. Through the establishment of the monitoring server, the compressive stress data can be displayed synchronously and the overpressure situation can be alarmed in real-time. The experimental results show that the accuracy of the calibrated sensor is within 1.6%, and the detection error of the acquisition board is less than 200 µV. The acquisition system is deemed to have considerable advantages in accuracy and applicability.

20.
J Med Chem ; 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36715603

RESUMO

Exploring multi-targeting chemotherapeutants with advantages over single-targeting agents and drug combinations is of great significance in drug discovery. Herein, we employed phytogenic evodiamine (EVO) and conventional Pt(II) drugs to design and synthesize multi-target EVO-Pt(IV) anticancer prodrugs (4-14). Among them, compound 10 exhibited a 118-fold enhancement in the IC50 value compared to cisplatin and low toxicity to normal cells. Further studies proved that 10 significantly enhanced intracellular Pt accumulation and DNA damage, perturbed mitochondrial membrane potential, inhibited cell migration and invasion, upregulated reactive oxygen species levels, and induced apoptosis and autophagic cell death. Molecular docking assay revealed that 10 fits perfectly into the extracellular signal-regulated protein kinase (ERK)-1 pocket, which was verified to produce profound ERK suppression. Most strikingly, compound 10 exhibited superior in vivo antitumor efficiency and effectively attenuated systemic toxicity. Our results emphasize that functionalizing platinum drugs with the multi-target EVO could generate synergistically excellent anticancer activity with low toxicity and decreased resistance, which may represent a brand-new cancer therapy modality.

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