Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.379
Filtrar
1.
Molecules ; 25(19)2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33019644

RESUMO

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

2.
BJOG ; 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33021043

RESUMO

OBJECTIVE: This study aimed to investigate the incidence and associated predictors of adverse pregnancy outcomes (APOs) among pregnant women infected with syphilis. DESIGN: Cox regression analysis. SETTING: China. POPULATION OR SAMPLE: Pregnant women who were tested and diagnosed with syphilis during the index pregnancy and delivered at a gestational age ≥28 weeks between 2016 and 2019. METHODS: Data were extracted from China's Information System of Prevention of Mother-to-Child Transmission (PMTCT) of Syphilis Management. Descriptive analysis provided profiles and pregnant outcomes of maternal syphilis, as well as the incidence of APOs. Log-rank tests and cox proportional hazard models were used to investigate factors influencing APOs in infected mothers with singleton births. MAIN OUTCOME MEASURES: The incidence of APOs; the Hazard Ratios (HRs) of associated predictors using cox proportional hazard model. RESULTS: Syphilis treatment data was available from 83.86% of diagnosed women. Including deliveries from the total study population, 13.33% experienced APOs. Cox regression indicated that APOs were more likely in women tested and diagnosed in the late trimester, delivery or postpartum. Women who accepted non-standardized treatment and who received standardized treatment had less risk of APOs . CONCLUSIONS: China has made huge progress over the last decades in PMTCT of syphilis, but the incidence of APOs among pregnant women infected with syphilis remains high. It is extremely essential to further strengthen access to early detection and standardized treatment of infected women to reduce the risk of APOs.

3.
Transbound Emerg Dis ; 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33048441

RESUMO

Anthrax is a natural foci disease in Inner Mongolia, which poses a severe threat to public health. In this study, the incidence number, rate, and constituent ratio were used to describe the epidemiological characteristics of anthrax in the region from 1956-2018. The molecular correlation and genetic characteristics of the strains were investigated using canonical single-nucleotide polymorphisms (CanSNP), multiple-locus variable-number tandem repeat analysis (MLVA-15), and whole genome sequencing (WGS). The epidemiological characteristics of anthrax in Inner Mongolia have altered significantly. The incidence of anthrax has decreased annually without vaccination, and the regional distribution of anthrax gradually transferred from central and western regions to the eastern. Moreover, the occupation distribution evolved from multiple early occupations to predominated by farmers and herdsmen. This change is closely its related to policy factors and to changes in the means of production and the living habits of the local population. This indicates that reformulate the control and prevention strategies is essential. Both A. Br. Ames and A. Br. 001/002 subgroups were the predominant CanSNP genotypes of Bacillus anthracis in Inner Mongolia. A total of 36 strains constituted six shared MLVA-15 genotypes, suggesting an epidemiological link between the strains of each shared genotype. The six shared genotypes ((GT1, 9, 11, and 15) and (GT8 and 12)) consisting of 2-7 strains confirmed the occurrence of multiple point outbreaks and cross-regional transmission caused by multiple common sources of infection. Phylogenetic analysis based on the WGS core genome showed that strains from this study formed an independent clade (C.V.), and they were positioned close to each other, suggesting a common origin. Further comparison analysis should be performed to ascertain the geographic origin of these strains.

4.
Lab Invest ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33005011

RESUMO

Odd-skipped related 1 (Osr1) is a novel tumor suppressor gene in several cancer cell lines. Non-alcoholic steatohepatitis (NASH) is considered as a high-risk factor for hepatocellular carcinoma (HCC). This study is aimed to investigate the novel role of Osr1 in promoting the progression of hepatic steatosis to NASH. Following 12 weeks of diethylnitrosamine (DEN) and high-fat diet (HFD), wildtype (WT) and Osr1 heterozygous (Osr1+/-) male mice were examined for liver injuries. Osr1+/- mice displayed worsen liver injury with higher serum alanine aminotransferase levels than the WT mice. The Osr1+/- mice also revealed early signs of collagen deposition with increased hepatic Tgfb and Fn1 expression. There was overactivation of both JNK and NF-κB signaling in the Osr1+/- liver, along with accumulation of F4/80+ cells and enhanced hepatic expression of Il-1b and Il-6. Moreover, the Osr1+/- liver displayed hyperphosphorylation of AKT/mTOR signaling, associated with overexpression of Bcl-2. In addition, Osr1+/- and WT mice displayed differences in the DNA methylome of the liver cells. Specifically, Osr1-responsible CpG islands of Ccl3 and Pcgf2, genes for inflammation and macrophage infiltration, were further identified. Taken together, Osr1 plays an important role in regulating cell inflammation and survival through multiple signaling pathways and DNA methylation modification for NAFLD progression.

5.
Front Immunol ; 11: 583274, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33072131

RESUMO

Background: A complex interplay between different cell types in the epithelium leads to activation of the luminal acidifying capacity of the epididymis, a process that is crucial for sperm maturation and storage. Basal cells sense the luminal angiotensin II (ANG II) and stimulate proton secretion in clear cells through nitric oxide (NO). Our previous study has shown the chemokine regulated upon activation normal T-cell expressed and secreted (RANTES) was expressed in the F4/80 positive macrophages of human epididymis. The objective of this study was to explore the involvement of RANTES in regulating the luminal acidification in the rat epididymis. Methods: The role of RANTES was investigated by in vivo perfusion with recombinant RANTES, Met-RANTES, and PBS of different pH values. Furthermore, rats vasectomy was performed to alter the epididymal luminal pH. RIA was used to measure the tissue homogenate ANG II concentration. Real time-PCR and western blot were employed to examine the expression levels of AGTR2, RANTES, CCR1, CCR5, and iNOS in epididymis. Results: RANTES was restricted to the basal macrophages of epididymal ducts and co-localized with its receptors CCR1 and CCR5. Both V-ATPase and iNOS were up-regulated in the cauda epididymis after perfused with recombinant RANTES, while the antagonist Met-RANTES perfusion led to a complete abrogation of the increased expression of V-ATPase in the apical membrane of clear cells and iNOS in macrophages. Upon alkaline perfusion, RANTES expression was significantly increased and the apical accumulation of V-ATPase in the clear cells was induced in the cauda epididymis. The luminal pH in the cauda epididymis increased after vasectomy. The concentration of the ANG II and the expression levels of AGTR2, RANTES, CCR1, CCR5, and iNOS dropped in the cauda epididymis following vasectomy. Conclusion: Upon the activation of basal cells, RANTES might induce the NO release from macrophages by interacting with its receptors, which increases proton secretion by adjacent clear cells. Thus, RANTES is possible to participate in the crosstalk among basal cells, macrophages and clear cells for the fine control of an optimum acidic luminal environment that is critical for male fertility.

6.
Sensors (Basel) ; 20(20)2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33080850

RESUMO

In order to reduce the vibration of mountain self-propelled electric monorail transporters (MSEMT) caused by the impact of the meshing of roller gear with toothed rail (MRGTR), and to improve the stability and safety of monorail transporters, this paper theoretically analyzed the MRGTR mechanism of toothed monorail transporters as well as established the MSEMT displacement model and its instantaneous velocity model. The vibration signals of MSEMT with four different parameters of toothed rail were collected by the acceleration sensor and signal acquisition system. The signals were analyzed by the Hilbert envelope demodulation method to investigate the influence of toothed rail parameters on meshing impact vibration. Moreover, taking the vibration acceleration amplitude of MSEMT and the vibration attenuation time of meshing impact as evaluation indexes, a test based on the three-factor and two-level orthogonal test was engaged with factors of toothed rail pressure angle, the ratio of L-the chord length of two adjacent roller centers of a roller gear-and rack pitch p (wheel-tooth ratio) and the load mass of the MSEMT. It showed that the impact of MRGTR was the main excitation source of the vibration of MSEMT. The pressure angle and wheel-tooth ratio both have a significant impact on the smooth operation of MSEMT, the latter to a greater extent. So did the interaction between wheel-tooth ratio and load mass. The amplitude of the characteristic frequency of the MSEMT decreased with the growth of the pressure angle. When the wheel-tooth ratio was cosα, the number of the characteristic frequency was less than that when it was 1, and the amplitude became smaller too. When the pressure angle was 15, the amplitude of vibration acceleration characteristic frequency decreased as a consequence of load mass increasing. At the pressure angle of 25, the amplitude of characteristic frequency decreased with the increase of load mass if the wheel-tooth ratio was 1, and the opposite result occurs in the case when the wheel-tooth ratio was cosα. This paper provides a theoretical basis and reference for improving the impact vibration of MRGTR and optimizing the design of the toothed rail.

7.
Med Eng Phys ; 85: 1-6, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33081956

RESUMO

There is an urgent clinical for small diameter vascular grafts (SDVGs) for use in the treatment of coronary artery disease. Moreover, biodegradable SDVGs are drawing increasing attention because they have the potential to restore vascular function with the degradation of vascular graft and tissue regeneration. In this study, we designed triple-layered SDVGs to mimic the native arterial structure, with each layer contributing its unique porosity to the porous structure. We evaluated triple-layered SDVGs in a sheep carotid arterial replacement model. After implantation for 12 months, two grafts were patent and indicated the feasibility of using grafts in large animals. Nevertheless, it was determined that both grafts formed aneurysms at the proximal end. The prevention of such aneurysms remains a challenge for future investigation.

8.
Anal Chem ; 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33086015

RESUMO

Unstable detection environment is one of the biggest interferences for in situ surface-enhanced Raman spectroscopy (SERS) using in real-time monitoring of toxic pollutants, leading to unreliable results. To address this problem, we have designed and prepared a cavity-based particle-in-quasicavity (PIQC) architecture composed of hierarchical ZnO/Ag nanosheets and nanoprotrusions for improving the in situ SERS performance under a liquid environment. Benefitting from the special cascaded optical field mode, the PIQC ZnO/Ag exhibits excellent in situ SERS detectability, with 10-18 M of limit of detection for rhodamine 6G and 12.8% of signal relative standard deviation value. Furthermore, by means of a microfluidic chip, this PIQC structure is proved to have the quantitative analysis feasibility and realizes real-time monitoring of the 3,3',4,4'-tetrachlorobiphenyl, a representative global environmental hazard, under the flowing environment. The strategy in this paper provides a brand new idea to promote the application of in situ SERS in contaminant monitoring and is also instructive for light control in other optical fields.

9.
J Clin Neurosci ; 80: 80-86, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33099373

RESUMO

OBJECTIVE: Postoperative fever (POF), associated with posterior cranial fossa (PCF) surgery, occurs commonly and is a potential intracranial infection indicator of perioperative antibiotics prolongation and advancement. The existing prophylactic approaches to balancing the risk between intracranial infection and antibiotics abuse are debatable. METHODS: We retrospectively assessed 100 patients subjected to PCF tumor resection between December 2015 and December 2018 at a single institution. Forty febrile patients were selected for further analysis. Of them, 16 received basic and 24 advanced antibiotics and were subjected to prophylactic antibiotic assessment. RESULTS: The total POF rate of PCF tumor resection was 49.4%. POF occurred from day 1 to day 5, along with the abnormalities of cerebrospinal fluid (CSF) profiles and the mild meningeal irritation symptom. CSF cultures of all selected patients were negative. In the comparison between the basic and advanced antibiotic therapy, we found no statistically significant differences in the results of the average and dynamic analysis of the body temperature and CSF profiles. Negative results of outcome studies were also obtained in the duration of fever, duration of hospitalization, and total hospitalization expenses. However, the expenses were substantially increased in the advanced antibiotic treatment. CONCLUSIONS: Although POF is a common symptom after PCF tumor resection, definite intracranial infection is rare. A high body temperature and significant abnormal CSF profiles at an early stage may not be a specific and sufficient indicator of intracranial infection to upgrade antibiotics therapy when standard prophylactic protocols have been accurately achieved.

10.
J Chem Theory Comput ; 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33095555

RESUMO

The ability to accurately predict the binding site, binding pose, and binding affinity for ligand-RNA binding is important for RNA-targeted drug design. Here, we describe a new computational method, RLDOCK, for predicting the binding site and binding pose for ligand-RNA binding. By developing an energy-based scoring function, we sample exhaustively all of the possible binding sites with flexible ligand conformations for a ligand-RNA pair based on the geometric and energetic scores. The model distinguishes from other approaches in three notable features. First, the model enables exhaustive scanning of all of the possible binding sites, including multiple alternative or coexisting binding sites, for a given ligand-RNA pair. Second, the model is based on a new energy-based scoring function developed here. Third, the model employs a novel multistep screening algorithm to improve computational efficiency. Specifically, first, for each binding site, we use a gird-based energy map to rank the binding sites according to the minimum Lennard-Jones potential energy for the different ligand poses. Second, for a given selected binding site, we predict the ligand pose using a two-step algorithm. In the first step, we quickly identify the probable ligand poses using a coarse-grained simplified energy function. In the second step, for each of the probable ligand poses, we predict the ligand poses using a refined energy function. Tests of the RLDOCK for a set of 230 RNA-ligand-bound structures indicate that RLDOCK can successfully predict ligand poses for 27.8, 58.3, and 69.6% of all of the test cases with the root-mean-square deviation within 1.0, 2.0, and 3.0 Å, respectively, for the top three predicted docking poses. The computational method presented here may enable the development of a new, more comprehensive framework for the prediction of ligand-RNA binding with an ensemble of RNA conformations and the metal-ion effects.

11.
Environ Res ; 192: 110260, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33069700

RESUMO

A plasmonic photocatalyst of AgBr/BiPO4/g-C3N4 was prepared. X-ray powder diffraction, Scanning electron microscope, Transmission electron microscopy, Fourier infrared spectroscopy, Ultraviolet Visible diffuse reflectance spectroscopy and photoluminescence emission spectra have been employed to determine the structure, morphology and optical property of the as-prepared AgBr/BiPO4/g-C3N4 composite and analysis the reasons for improving photocatalytic efficiency. The optimal doping ratio of AgBr was 10 wt% by degrading 20 mg/L of Reactive Blue 19 (RB19) under visible light (λ > 420 nm), and 10 wt%AgBr/BiPO4/g-C3N4 degraded 20 mg/L of RB19 to 2.59% at 40 min, which is ascribed to synergistic effects at the interface of AgBr, BiPO4 and g-C3N4. The effect of catalyst dosage, initial concentration and initial pH of RB19 solution on photocatalytic efficiency was investigated. Four cycles of experiments were conducted. Finally, through the trapping experiment, we found that the main active factor for degrading RB19 in the photocatalytic process is O2-. The possible photocatalytic mechanism of AgBr/BiPO4/g-C3N4 was discussed in connection with the synergistic effect of Ag and active substances at the AgBr/BiPO4/g-C3N4 interface.

12.
Sci Rep ; 10(1): 17855, 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33082492

RESUMO

Lifestyle-induced weight loss is regarded as an efficient therapy to reverse metabolic syndrome (MetS) and to prevent disease progression. The objective of this study was to investigate whether lifestyle-induced weight loss modulates gene expression in circulating monocytes. We analyzed and compared gene expression in monocytes (CD14+ cells) and subcutaneous adipose tissue biopsies by unbiased mRNA profiling. Samples were obtained before and after diet-induced weight loss in well-defined male individuals in a prospective controlled clinical trial (ICTRP Trial Number: U1111-1158-3672). The BMI declined significantly (- 12.6%) in the treatment arm (N = 39) during the 6-month weight loss intervention. This was associated with a significant reduction in hsCRP (- 45.84%) and circulating CD14+ cells (- 21.0%). Four genes were differentially expressed (DEG's) in CD14+ cells following weight loss (ZRANB1, RNF25, RB1CC1 and KMT2C). Comparative analyses of paired CD14+ monocytes and subcutaneous adipose tissue samples before and after weight loss did not identify common genes differentially regulated in both sample types. Lifestyle-induced weight loss is associated with specific changes in gene expression in circulating CD14+ monocytes, which may affect ubiquitination, histone methylation and autophagy.

13.
Molecules ; 25(20)2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33092290

RESUMO

Conventional treatments for chronic periodontitis are less effective in controlling inflammation and often relapse. Therefore, it is necessary to explore an immunomodulatory medication as an adjuvant. Ginsenoside Rb3 (Rb3), one of the most abundant active components of ginseng, has been found to possess anti-inflammatory and immunomodulatory properties. Here, we detected the anti-inflammatory effect of Rb3 on Porphyromonas gingivalis LPS-stimulated human periodontal ligament cells and experimental periodontitis rats for the first time. We found that the expression of pro-inflammatory mediators, including IL-1ß, IL-6 and IL-8, upregulated by lipopolysaccharide (LPS) stimulation was remarkably downregulated by Rb3 treatment in a dose-dependent manner at both transcriptional and translational levels. Network pharmacological analysis of Rb3 showed that the mitogen-activated protein kinase (MAPK) signaling pathway had the highest richness and that p38, JNK, and ERK molecules were potential targets of Rb3 in humans. Western blot analysis revealed that Rb3 significantly suppressed the phosphorylation of p38 MAPK and p65 NF-κB, as well as decreased the expression of total AKT. In experimental periodontitis rat models, reductions in alveolar bone resorption and osteoclast generation were observed in the Rb3 treatment group. Thus, we can conclude that Rb3 ameliorated Porphyromonas gingivalis LPS-induced inflammation by inhibiting the MAPK/AKT/NF-κB signaling pathways and attenuated alveolar bone resorption in experimental periodontitis rats.

14.
J Microbiol ; 2020 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-33095389

RESUMO

The Gram-positive bacterium Enterococcus faecalis is currently one of the major pathogens of nosocomial infections. The lifestyle of E. faecalis relies primarily on its remarkable capacity to face and survive in harsh environmental conditions. Toxin-antitoxin (TA) systems have been linked to the growth control of bacteria in response to adverse environments but have rarely been reported in Enterococcus. Three functional type II TA systems were identified among the 10 putative TA systems encoded by E. faecalis ATCC29212. These toxin genes have conserved domains homologous to MazF (DR75_1948) and ImmA/IrrE family metallo-endopeptidases (DR75_1673 and DR75_2160). Overexpression of toxin genes could inhibit the growth of Escherichia coli. However, the toxin DR75_1673 could not inhibit bacterial growth, and the bacteriostatic effect occurred only when it was coexpressed with the antitoxin DR75_1672. DR75_1948-DR75_1949 and DR75_160-DR75_2161 could maintain the stable inheritance of the unstable plasmid pLMO12102 in E. coli. Moreover, the transcription levels of these TAs showed significant differences when cultivated under normal conditions and with different temperatures, antibiotics, anaerobic agents and H2O2. When DR75_2161 was knocked out, the growth of the mutant strain at high temperature and oxidative stress was limited. The experimental characterization of these TAs loci might be helpful to investigate the key roles of type II TA systems in the physiology and environmental stress responses of Enterococcus.

15.
J Int Med Res ; 48(10): 300060520964340, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33100072

RESUMO

BACKGROUND: Triple-negative breast cancer (TNBC) is associated with higher aggressiveness and mortality than hormone-positive breast cancer because of the lack of approved therapeutic targets. Patients with TNBC who attain a pathological complete response (pCR) after neoadjuvant chemotherapy have improved survival. Platinum-based agents show promising activity in TNBC; however, their use remains controversial. We conducted a meta-analysis to assess the role of platinum-based agents in neoadjuvant chemotherapy in patients with TNBC. METHODS: We performed an extensive literature search of the Pubmed, Embase, and Cochrane databases. We calculated pooled odds ratios (OR) with 95% confidence intervals (CI) for the identified studies. RESULTS: Eight randomized controlled trials with 1345 patients were included in the analysis. The addition of platinum-based agents improved pCR compared with neoadjuvant therapy based on anthracyclines, cyclophosphamide, taxanes, and fluorouracil (49.1% vs. 35.9%; OR: 1.87, 95% CI: 1.23-2.86). Hematological adverse events were similar in both groups, except for more thrombocytopenia in the platinum-based group (OR: 7.96, 95% CI: 3.18-19.93). CONCLUSION: The addition of platinum-based agents to neoadjuvant chemotherapy improved pCR rates in patients with TNBC, with a slight increase in hematological toxicities. Platinum-based agents might thus be an accessible and economically viable option in patients with TNBC.

16.
Biomed Res Int ; 2020: 2892734, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33102576

RESUMO

Background: Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are widely spread across the world. Asymptomatic or inconspicuous CT/NG infections are difficult to diagnose and treat. Traditional methods have the disadvantages of low detection rate, inaccurate results, and long detection time. However, Xpert CT/NG makes up for the aforementioned shortcomings and has research value and popularization significance. Methods: PubMed, Embase, Cochrane Library, and Web of Science were systematically searched, and studies were screened using Xpert CT/NG for diagnosing CT/NG. QUADAS-2 was used to evaluate the quality of the eligible studies. Then, two groups of researchers independently extracted data from these studies. Meta-analyses of sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and the area under the curve (AUC) of the summary receiver operating characteristic (SROC) curve were conducted using Meta-DiSc 1.4. Finally, Deek's funnel plots were made using Stata 12.0 to evaluate publication bias. Results: 14 studies were identified, and 46 fourfold tables were extracted in this meta-analysis. The pooled SEN, SPE, PLR, NLR, DOR, and AUC in diagnosing CT were 0.94 (95% confidence interval (CI): 0.93-0.95), 0.99 (95% CI: 0.99-1.00), 97.17 (95% CI: 56.76-166.32), 0.07 (95% CI: 0.04-0.12), 1857.25 (95% CI: 943.78-3654.86), and 0.9960, respectively. The pooled SEN, SPE, PLR, NLR, DOR, and AUC in diagnosing NG were 0.95 (95% CI: 0.93-0.96), 1.00 (95% CI: 1.00-1.00), 278.15 (95% CI: 152.41-507.63), 0.08 (95% CI: 0.06-0.12), 4290.70 (95% CI: 2161.78-8516.16), and 0.9980, respectively. Conclusions: Xpert CT/NG had high diagnostic sensitivity and specificity for CT and NG. However, more evidence is required to confirm that Xpert CT/NG might serve as the primary method for detecting CT and NG and even the gold standard for diagnosis in the future.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33006481

RESUMO

Background: Circular RNAs (circRNAs) are regarded as important regulators in the tumorigenesis of multiple cancers. However, the characterization of circRNA exocyst complex component 6B (circEXOC6B) in ovarian cancer is barely known. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to detect the enrichment of circEXOC6B, microRNA-376c-3p (miR-376c-3p), and forkhead box O3 (FOXO3). Cell proliferation was examined by Cell Counting Kit-8 (CCK8) assay and colony formation assay. Cell metastasis was measured by transwell assays. Western blot assay was conducted to examine the expression of proliferation and metastasis-related proteins and FOXO3. The chemoresistance of ovarian cancer cells was analyzed by CCK8 assay. Flow cytometry was used to detect cell apoptosis. The activities of caspase3 and caspase9 were analyzed through using colorimetric assay kits. The direct interaction between miR-376c-3p and circEXOC6B or FOXO3 was predicted by StarBase software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Murine xenograft assay was conducted to verify the role of circEXOC6B on the paclitaxel (PTX) resistance of ovarian cancer cells in vivo. Results: The level of circEXOC6B was notably decreased in ovarian cancer tissues. Low level of circEXOC6B was associated with malignant pathological characteristics in ovarian cancer patients. CircEXOC6B suppressed the proliferation and motility and decreased the chemoresistance of ovarian cancer cells to PTX. CircEXOC6B functioned through directly targeting and downregulating miR-376c-3p. FOXO3 was a direct target of miR-376c-3p, and the abundance of FOXO3 was regulated by circEXOC6B/miR-376c-3p axis. CircEXOC6B accelerated the PTX sensitivity of ovarian cancer cells through acting as a decoy of miR-376c-3p to upregulate FOXO3 in vivo. Conclusion: CircEXOC6B suppressed the progression and PTX resistance of ovarian cancer cells through sequestering miR-376c-3p, thus enhancing FOXO3 level.

18.
Artigo em Inglês | MEDLINE | ID: mdl-33027006

RESUMO

With the rapid development of sensor technologies, multisensor signals are now readily available for health condition monitoring and remaining useful life (RUL) prediction. To fully utilize these signals for a better health condition assessment and RUL prediction, health indices are often constructed through various data fusion techniques. Nevertheless, most of the existing methods fuse signals linearly, which may not be sufficient to characterize the health status for RUL prediction. To address this issue and improve the predictability, this article proposes a novel nonlinear data fusion approach, namely, a shape-constrained neural data fusion network for health index construction. Especially, a neural network-based structure is employed, and a novel loss function is formulated by simultaneously considering the monotonicity and curvature of the constructed health index and its variability at the failure time. A tailored adaptive moment estimation algorithm (Adam) is proposed for model parameter estimation. The effectiveness of the proposed method is demonstrated and compared through a case study using the Commercial Modular Aero-Propulsion System Simulation (C-MAPSS) data set.

19.
J Neural Eng ; 17(5): 056021, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33052888

RESUMO

OBJECTIVE: The data scarcity problem in emotion recognition from electroencephalography (EEG) leads to difficulty in building an affective model with high accuracy using machine learning algorithms or deep neural networks. Inspired by emerging deep generative models, we propose three methods for augmenting EEG training data to enhance the performance of emotion recognition models. APPROACH: Our proposed methods are based on two deep generative models, variational autoencoder (VAE) and generative adversarial network (GAN), and two data augmentation ways, full and partial usage strategies. For the full usage strategy, all of the generated data are augmented to the training dataset without judging the quality of the generated data, while for the partial usage, only high-quality data are selected and appended to the training dataset. These three methods are called conditional Wasserstein GAN (cWGAN), selective VAE (sVAE), and selective WGAN (sWGAN). MAIN RESULTS: To evaluate the effectiveness of these proposed methods, we perform a systematic experimental study on two public EEG datasets for emotion recognition, namely, SEED and DEAP. We first generate realistic-like EEG training data in two forms: power spectral density and differential entropy. Then, we augment the original training datasets with a different number of generated realistic-like EEG data. Finally, we train support vector machines and deep neural networks with shortcut layers to build affective models using the original and augmented training datasets. The experimental results demonstrate that our proposed data augmentation methods based on generative models outperform the existing data augmentation approaches such as conditional VAE, Gaussian noise, and rotational data augmentation. We also observe that the number of generated data should be less than 10 times of the original training dataset to achieve the best performance. SIGNIFICANCE: The augmented training datasets produced by our proposed sWGAN method significantly enhance the performance of EEG-based emotion recognition models.

20.
Ann Palliat Med ; 2020 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-33040539

RESUMO

Benign metastasizing leiomyoma (BML) is a rare condition that occurs mainly in premenopausal women and is characterized most commonly by pulmonary metastases. Here, we report the case of a 45-yearold woman who presented with multiple bilateral pulmonary nodules on chest examination during a health checkup 13 years after myomectomy. This patient has a normal menstrual cycle, moderate anemia, and no obvious respiratory symptoms. Serum concentrations of cancer markers such as carcinoembryonic antigen, neuron specific enolase, cytokeratin 19 fragments, and pro-gastrin-releasing peptide were within normal limits. Color doppler ultrasound was also performed, several hypoechoic regions were found in uterine bodies and cavity. The computed tomography (CT)-guided lung biopsy was used for histopathological examination. Immunohistochemical staining revealed BML which were positive for smooth muscle antibody, desmin, vimentin, estrogen and progesterone receptors, and Ki-67 positive rate of about 1%. Hysterectomy and bilateral adnexectomy were performed as a part of treatment. The lung nodules were meticulously monitored at follow-up. Three months later, the repeat CT scan showed that the nodules had reduced in size, and no new nodules had appeared, 1 year later, CT scan showed no obvious changes in lung nodules. This study is of great significance as the results will be helpful in diagnosing and treating future pulmonary benign metastasizing leiomyoma (PBML) cases.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA