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1.
J Hazard Mater ; 423(Pt A): 127088, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34482077

RESUMO

Antibiotic resistance has become a global public health problem. Recently, various environmental pollutants have been reported to induce the proliferation of antibiotic resistance. However, the impact of multiple pollutants (e.g., heavy metals and antibiotics), which more frequently occur in practical environments, is poorly understood. Herein, one widely distributed heavy metal (Ag+) and one frequently detected antibiotic (tetracycline) were chosen to investigate their coexisting effect on the proliferation of antibiotic resistance in the activated sludge system. Results show that the co-occurrence of Ag+ and tetracycline at environmentally relevant concentrations exhibited no distinct inhibition in reactor performances. However, they inhibited the respiratory activity by 42%, destroyed the membrane structure by 218%, and increased membrane permeability by 29% compared with the blank control bioreactor. Moreover, the relative abundances of target antibiotic resistance genes (ARGs) (e.g., tetA, blaTEM-1, and sulII) in effluent after exposure of coexisting Ag+ and tetracycline were increased by 92-1983% compared with those in control reactor, which were 1.1-4.3 folds higher than the sum of the sole ones. These were possibly attributed to the enrichments of antibiotic-resistant bacteria. The results would illumine the coexisting effect of heavy metals and antibiotics on the dissemination of ARGs in activated sludge system.

2.
EBioMedicine ; 71: 103558, 2021 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-34521054

RESUMO

BACKGROUND: Resistance to platinum-based chemotherapy is a major cause of therapeutic failure during the treatment of epithelial ovarian cancer (EOC) patients. Our study aims to elucidate the molecular mechanisms by which ZNF711 down regulation promotes CISPLATIN resistance in EOC. METHODS: ZNF711 expression in 150 EOC specimens was examined using immunohistochemistry. ZNF711 expression and the survival of EOC patients were assessed with a Kaplan-Meier analysis. The effects of ZNF711 expression on CDDP resistance were studied by IC50, Annexin V, and colony formation in vitro, and in an in vivo intra-peritoneal tumor model. The molecular mechanism was determined using a luciferase reporter assay, ChIP assay, CAPTURE approach, and co-IP assay. FINDINGS: ZNF711 down-regulation exerts a great impact on CDDP resistance for EOC patients by suppressing SLC31A1 and inhibiting CDDP influx. ZNF711 down-regulation promoted, while ZNF711 overexpression drastically inhibited CDDP resistance, both in vivo and in vitro. Mechanistically, the histone demethylase JHDM2A was recruited to the SLC31A1 promoter by ZNF711 and decreased the H3K9me2 level, resulting in the activation of SLC31A1 transcription and enhancement of CDDP uptake. Importantly, co-treatment with the histone methylation inhibitor, BIX-01294, increased the therapeutic efficacy of CDDP treatment in ZNF711-suppressed EOC cells. INTERPRETATION: These findings both verified the clinical importance of ZNF711 in CDDP resistance and provide novel therapeutic regimens for EOC treatment. FUNDING: This work was supported by the Natural Science Foundation of China; Guangzhou Science and Technology Plan Projects; Natural Science Foundation of Guangdong Province; The Fundamental Research Funds for the Central Universities; and China Postdoctoral Science Foundation.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34465137

RESUMO

Yunnan is the first place where HIV-1 became prevalent in China, and it is also the place with the most complicated HIV-1 genetic diversity in China. On October 23, 2019, a patient newly diagnosed with acquired immunodeficiency syndrome from a hospital in Baosan, Yunnan, was recruited for genetic analysis. Near full-length genome of HIV-1 was amplified from the plasma sample. Phylogenetic analysis revealed that this sequence (BS6F24) has a close relationship with CRF86_BC and a unique recombinant form (KY406739), which was formed by recombination of subtypes B and C. Bootscan analysis confirmed that the first part (HXB2:1022-5832) and last part (HXB2:5833-9120) genomes of BS6F24 had the same recombinant structures as KY406739 and CRF86_BC, respectively. A second-generation recombinant form that originated from CRF86_BC and a unique recombinant form were reported for the first time. This indicates the need for continuous monitoring of the genetic diversity of HIV-1 in Yunnan, China.

4.
J Adv Nurs ; 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34468043

RESUMO

AIMS: To examine the distributed characteristics and explore the research themes of Doctor of Nursing Practice (DNP) dissertations during the past two decades. DESIGN: A descriptive statistical and visualization bibliometric analysis was conducted. METHODS: Doctor of Nursing Practice dissertations submitted between January 2005 and June 2021 were collected from the ProQuest Dissertations and Theses database. A descriptive statistical analysis was conducted to calculate the distribution of the DNP dissertations by granting institution and the published year of publications. The VOSviewer 1.6.13 was used to explore the bibliometric networks and research priorities of the DNP dissertations. RESULTS: A total of 4989 DNP dissertations from 90 universities were included in this study, all from the United States. The number of DNP dissertations showed an upward trend, with steady growth from 2005 to 2014 and rapid growth after 2015. The DNP studies focused on five areas: health care management in clinical nursing, advanced practice in nursing education and health education, public health problems, mental health care for adolescents and nurses and the older people care and long-term care. CONCLUSION: Parallel to the numerical increase in DNP dissertations is a steady expansion in the range of research topics and scopes, which is aligned with specific specializations of the DNP. Many are interdisciplinary and employ techniques imported from the fields of public health, psychology and social sciences, resulting in nursing educators and practitioners continually broaden their subject perspectives. IMPACT: Knowing where, when and why DNP research trends developed will help nursing educators to further develop DNP education and optimize DNP programs in the future, such as paying more attention to the nursing practice. Moreover, this study will inspire DNP students and researchers to expand their subject perspectives and broaden the research scope to solve nursing practice problems based on interdisciplinary theories and methods.

5.
Neuro Endocrinol Lett ; 42(5): 331-338, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34506097

RESUMO

OBJECTIVE: The purpose of this study was to investigated whether pretreated with Atorvastatin be helpful in diabetic or wild-type mice, and clarify the possible mechanisms. METHODS: C57/B6 and ob/ob mice treated with atorvastatin or not were subjected to middle cerebral artery occlusion (MCAO), which were killed after 2h of occlusion following by 22h of reperfusion. We used Neurological Severity Scores (NSS) to assess the severity of brain injury, and TTC staining was used to measure the infraction volume. Protein levels of PGC-1α, vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), Bcl2, Bax and signaling pathway protein of mitogen-activated protein kinase (MAPK) were estimated by western blot. RESULTS: Atorvastatin could slake the cerebral ischemic/ reperfusion injury in ob/ob diabetic mice, but do nothing on wild-type mice. The expression of PGC-1α and related angiogenic factors such as VEGF and Ang-1 were lower in the diabetic mice after MCAO than wild-type, which could be effective reversed by atorvastatin pretreatment before MCAO. This may be one of the possible mechanisms for atorvastatin to alleviate ischemic injury. MAPK pathway and apoptosis-related proteins were also involved in this course. CONCLUSION: Impaired angiogenesis mediated by PGC-1α plays an important role in exacerbating ischemic cerebral insults in diabetic mice, and pretreatment with atorvastatin before MCAO has a protective effect through the regulation of PGC-1α and angiogenic factors.

6.
Biotechnol J ; : e2100176, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34506679

RESUMO

Viral surrogates to screen for virus inactivation (VI) can be a faster, cheaper and safer alternative to third-party testing of pathogenic BSL2 (Biosafety level 2) model viruses. Although the bacteriophage surrogate, Ø6, has been used to assess low pH BSL2 VI, it has not been used for evaluation of detergent-mediated VI. Furthermore, Ø6 is typically assayed through host cell infectivity which introduces the risk of cross-contaminating other cell lines in the facility. To circumvent contamination, we developed an in-house RT-qPCR (Reverse transcriptase quantitative polymerase chain reaction) assay for selective detection of active Ø6 from a population of live and dead phage. The RT-qPCR assay was used to evaluate Ø6 inactivation in cell culture fluid of monoclonal antibody and fusion protein. Complementary Ø6 infectivity was also conducted at a third-party testing facility. The Ø6 RT-qPCR and infectivity data was modeled against VI of three BSL2 viruses, X- MuLV, A- MuLV and HSV-1 in corresponding therapeutics. Both Ø6 methods demonstrate that any VI agent showing Ø6 clearance of a minimum of 2.5 logs would demonstrate complete BSL2 VI of ≥ 4.0 logs. Compared to BSL2 virus testing, this in-house Ø6 RT-qPCR tool can screen VI agents at 5% the cost and a turnaround time of 2 to 3 days versus 4 to 7 months. This article is protected by copyright. All rights reserved.

7.
IEEE Trans Biomed Eng ; PP2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34516370

RESUMO

OBJECTIVE: Stroke survivors are usually unable to perform activities of daily living (ADL) independently due to loss of hand functions. Soft pneumatic gloves provide a promising assistance approach for stroke survivors to conduct ADL tasks. However, few studies have explored effective control strategies for the 'human-soft robot' integrated system due to challenges in the nonlinearities of soft robots and uncertainties of human intentions. Therefore, this work aims to develop control approaches for the system to improve stroke survivors hand functions. METHODS: Firstly, a soft pneumatic glove was utilized to aid with stroke-impaired hands. Secondly, a probabilistic model-based learning control approach was proposed to overcome the challenges. Then a task-oriented intention-driven training modality was designed. Finally, the control performance was evaluated on three able-bodied subjects and three stroke survivors who attended 20-session rehabilitation training. RESULTS: The proposed approach could enable the soft pneumatic glove to provide adaptive assistance for all participants to accomplish different tasks. The tracking error and muscle co-contraction index showed decreasing trends while the hand gesture index showed an increasing tendency over training sessions. All stroke survivors showed improved hand functions and better muscle coordination after training. CONCLUSION: This work developed a learning-based soft robotic glove training system and demonstrated its potential in post-stroke hand rehabilitation. SIGNIFICANCE: This work promotes the application of soft robotic training systems in stroke rehabilitation.

8.
Nanoscale ; 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34523632

RESUMO

The transport direction of water droplets on a functionalized surface is of great significance due to its wide applications in microfluidics technology. The prevailing view is that a water droplet on a wedge-shaped groove always moves towards the wider end. In this paper, however, molecular dynamics simulations show that a water droplet can move towards the narrower end if placed at specific positions. It is found that the direction of water droplet transport on a grooved surface is related to its initial position. The water droplet moves towards the wider end only when it is placed near the wider end initially. If the water droplet is placed near the narrower end, it will move in the opposite direction. The novel phenomenon is attributed to the opposite interactions of the groove substrate and the groove upper layers with water droplets. Two effective models are proposed to exploit the physical origin of different transport directions of water droplets on a wedge-shaped groove surface. The study provides an insight into the design of nanostructured surfaces to effectively control the droplet motion.

9.
J Environ Manage ; 299: 113641, 2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34479150

RESUMO

Aerobic denitrifying bacteria have the potential to remove the co-pollutants Ni(II) and nitrate in industrial wastewater. In this study, aerobic denitrifying bacteria with significant Ni(II) removal efficiency was isolated from the biological reaction tank and named as Pseudomonas hibiscicola L1 strain after 16 S rRNA identification analysis. The removal of ever-increasing Ni(II) and NO3--N wastewater under aerobic conditions by strain L1 was discussed. The experimental results showed that strain L1 removed 84% of Ni(II) and 81% of COD, with the use of 34.8 mg L-1 of nitrogen source and without nitrite accumulation yet. Strain L1 had remarkable activity (OD600 = 0.51-0.56 (p < 0.05)) at 20 mg L-1 of Ni(II) and 100 mg L-1 of NO3--N. It was found that high Ni(II) gradients (2-10 mg L-1) had little effect on nitrate removal ratio (35-34% (p > 0.05), and the removal ratios of Ni(II) was enhanced (from 42% to 83% (p < 0.05)) by increasing nitrate (25-100 mg L-1). Also, the results indicated that strain L1 could reduce Ni(II) and nitrate under different pH (6-9); electron donor-glucose, sodium acetate, sodium succinate and trisodium citrate; C/N (5-20) and coexisting ions (Cu(II) and Zn(II)). Notably, the nitrogen balance analysis showed 32.4% of TN was lost nitrogen and 19.7% of TN was assimilated for cell growth, which indicated aerobic denitrification process of strain L1. Meanwhile, characterization technology (SEM, FTIR, and XRD) showed Ni(II) was bioadsorbed in the form of Ni(NH2)2, NiCO3, and Ni(OH)2·2H2O through surface functional groups. This research provides new microbial method for the simultaneous removal of nitrate and Ni(II) in wastewater.

10.
Gene ; : 145948, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34481002

RESUMO

BACKGROUNDS: To investigate associations of genetic factors and environmental factors with coronary artery disease (CAD), we collected medical reports, lifestyle details, and blood samples of 2113 individuals, and then used the polymerase chain reaction (PCR)-ligase detection reaction (LDR) to genotype the targeted 102 SNPs. METHODS: We adopted elastic net algorithm to build an association model that considered simultaneously genetic and lifestyle/clinical factors associated with CAD in Chinese Han population. RESULTS: In this study, we developed an all covariates-based model to explain the risk of CAD, which incorporated 8 lifestyle/clinical factors and a gene-score variable calculated from 3 significant SNPs (rs671, rs6751537 and rs11641677), attaining an area under the curve (AUC) value of 0.71. It is found that, in terms of genetic variants, the AA genotype of rs671 in the additive (adjusted odds ratio (OR)=2.51, p=0.008) and recessive (adjusted OR=2.12, p=0.021) models, the GG genotype of rs6751537 in the additive (adjusted OR=3.36, p=0.001) and recessive (adjusted OR=3.47, p=0.001) models, and GG genotype of rs11641677 in additive model (adjusted OR=0.39, p=0.044) was associated with increased risk of CAD. In terms of lifestyle/clinical factors, the history of hypertension (unadjusted OR=2.37, p<0.001) and dyslipidemia (unadjusted OR=1.82, p=0.007), age (unadjusted OR=1.07, p<0.001) and waist circumference (unadjusted OR=1.02, p=0.05) would significantly increase the risk of CAD, while height (unadjusted OR=0.97, p=0.006) and regular intake of chicken (unadjusted OR=0.78, p=0.008) reduced the risk of CAD. A significantinteraction was foundbetween rs671 and dyslipidemia (the relative excess risk due to interaction (RERI) = 3.36, p=0.05). CONCLUSION: In this study, we constructed an association mode and identified a set of SNPs and lifestyle/clinical risk factors of CAD in Chinese Han population. By considering both genetic and non-genetic risk factors, the built model may provide implications for CAD pathogenesis and clues for screening tool development in Chinese Han population.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34478216

RESUMO

A cooperative OSDA strategy is demonstrated, leading to novel high-silica FAU zeolites with a large potential for disruptive acid catalysis. In bottom-up synthesis, the symbiosis of choline ion (Ch + ) and 15-crown-5 (CE) was evidenced, in a form of full occupation of the sodalite ( sod ) cages with the trans Ch + conformer, induced by the CE presence. CE itself occupied the supercages along with additional gauche Ch + , but in synthesis without CE, no trans was found. The cooperation, and thus the fraction of trans Ch + , was closely related to the Si/Al ratio, a key measure for FAU stability and acidity. As such, a bottom-up handle for lowering the Al-content of FAU and tuning its acid site distribution is shown. A mechanistic study demonstrated that forming sod cages with trans Ch + is key to the nucleation of high-silica FAU zeolites. The materials showed superior performances to commercial FAU zeolites and those synthesized without cooperation, in the catalytic degradation of polyethylene.

13.
Oncogene ; 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34535770

RESUMO

Liver metastasis (LM) severely affects gastric cancer (GC) patients' prognosis. Small extracellular vesicles (sEVs) play key roles in intercellular communication. Specific sEV-miRNAs from several types of cancer were found to induce a premetastatic niche in target organs before tumor cell arrive. However, whether the primary GC affects hepatic microenvironment or the role of sEV-miRNAs in GC-LM is yet unclear. We report that GC-derived sEVs are primarily absorbed by Kupffer cells (KCs). sEV-miR-151a-3p is highly expressed in GC-LM patients' plasma and presents poor prognosis. Treating mice with sEVs-enriched in miR-151a-3p promotes GC-LM, whereas has no influence on the proliferation of GC cells in situ. Mechanistically, sEV-miR-151a-3p inhibits SP3 in KCs. Simultaneously, sEV-miR-151a-3p targets YTHDF3 to decrease the transcriptional inhibitory activity of SP3 by reducing SUMO1 translation in a N6-methyladenosine-dependent manner. These factors contribute to TGF-ß1 transactivation in KCs, subsequently activating the SMAD2/3 pathway and enhancing the stem cell-like properties of incoming GC cells. Furthermore, sEV-miR-151a-3p induces miR-151a-3p transcription in KCs to form a positive feedback loop. In summary, our results reveal a previously unidentified regulatory axis initiated by sEV-miR-151a-3p that establishes a hepatic stemness-permissive niche to support GC-LM. sEV-miR-151a-3p could be a promising diagnostic biomarker and preventive treatment candidate for GC-LM.

14.
Rapid Commun Mass Spectrom ; : e9200, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34532912

RESUMO

RATIONALE: Succus Bambusae is consumed as a kind of herbal medicine and natural beverage in China. However, the current quality standards of Succus Bambusae are low and lack of safety indicators, which makes it difficult to effectively guarantee its quality. Therefore, it is of great significance to study the identification and quality control technology for the product. METHODS: In this research, A set of qualitative and quantitative methods based on gas chromatography-mass spectrometry (GC-MS) was developed for the analysis of volatile components in Succus Bambusae oral liquid (SBOL). Combining GC-MS fingerprint analysis and related chemometrics algorithms, with similarity evaluation, Hotelling T2 and distance to Model X (DModX) as criteria, the quality consistency of different batches was evaluated, and the SBOL samples from different manufacturers were differentiated. RESULTS: 29 volatile components were preliminarily identified from 40 batches of SBOL samples from 6 manufacturers, and 6 Q-markers (Quality Markers) for the SBOLs was disscussed and determined using GC-MS. The products from different manufacturers were distinguished using Chemometrics. CONCLUSIONS: The results showed that the quality of the SBOL samples of different batches and different manufacturers fluctuated greatly, which suggested that the researches of raw materials and manufacturing techniques should be strengthened to improve the quality of SBOL and ensure its quality consistency.

15.
J Hepatol ; 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34509526

RESUMO

BACKGROUND & AIMS: Intrahepatic cholangiocarcinoma (ICC) is a primary liver cancer with high aggressiveness and extremely poor prognosis. The role of circular RNAs (circRNAs) in ICC carcinogenesis and progression remains to be determined. METHODS: CircRNA microarray was performed to screen significantly up-regulated circRNAs in paired ICC and non-tumor tissues. Colony formation, transwell, and xenograft models were used to examine the role of circRNAs in ICC proliferation and metastasis. RNA pulldown, mass spectrometry, chromatin immunoprecipitation, RNA binding protein immunoprecipitation, chromatin isolation by RNA purification, electrophoretic mobility shift assay, and luciferase reporter assays were used to explore the molecular sponge role of the circRNA via binding to miRNAs, and the interaction between circRNA and RNA-binding proteins. RESULTS: Hsa_circ_0050898, which originated from exon 1 to exon 20 of the ACTN4 gene (named as circACTN4), was significantly upregulated in ICC. High circACTN4 expression was associated with enhanced tumor proliferation and metastasis in vitro and in vivo, as well as a worse prognosis following ICC resection. In addition, circACTN4 upregulated Yes-associated protein1 (YAP1) expression by sponging miR-424-5p. More importantly, circACTN4 also recruited Y-box binding protein 1 (YBX1) to stimulate Frizzled-7 (FZD7) transcription. Furthermore, circACTN4 overexpression in ICC cells enhanced the interaction between YAP1 and ß-catenin, which are the core components of the Hippo and Wnt signaling pathways, respectively. CONCLUSIONS: CircACTN4 was upregulated in ICC and promoted ICC proliferation and metastasis by acting as a molecular sponge of miR-424-5p, as well as by interacting with YBX1 to transcriptionally activate FZD7. These results suggested that circACTN4 is a potential prognostic marker and therapeutic target for ICC. LAY SUMMARY: A circular RNA (circACTN4) was highly expressed in intrahepatic cholangiocarcinoma (ICC). The expression level of circACTN4 was positively associated with tumor growth and metastasis through both the Hippo and Wnt signaling pathways.

16.
J Hazard Mater ; 416: 125865, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492813

RESUMO

Anaerobic biological treatment technologies are one of the major hotspots of antibiotic resistance genes (ARGs). Previous studies have applied the electrochemical process to improve biogas production, however, it was challenged that high voltages might promote membrane permeability and reactive oxygen species overproduction to promote ARGs proliferation. Herein, the biogas production and ARGs proliferation in an anaerobic electrochemical membrane bioreactor (AnEMBR) were investigated at the gradient voltages of 0-0.9 V. Results show the reactor performances (average CH4 production and current generation) were distinctly improved with the increase of applied voltage, and reached the optimum at 0.9 V. However, long-term application (>30 day) of 0.9 V deteriorated the reactor performances. Meanwhile, the relative abundances of most target ARGs in the supernatant and effluent of AnEMBR at 0.9 V increased by 0.68-1.55 and 0.42-1.26 logs compared to those before applying voltage, respectively. After disconnecting the circuit, these ARGs abundances all decreased to the original level. Significant correlations between intlI and ARGs (e.g., tetA, tetQ, sulI, and sulII) were observed, indicating horizontal gene transfer may contribute to the increased ARGs. Moreover, the shift of microbial communities caused by the applied voltage enriched potential ARGs-hosts (e.g., Tolumonas), contributing to the proliferation of ARGs.


Assuntos
Antibacterianos , Biocombustíveis , Anaerobiose , Antibacterianos/farmacologia , Reatores Biológicos , Proliferação de Células , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos
17.
Biomed Environ Sci ; 34(9): 705-718, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34530960

RESUMO

Objective: The effects of interactions between genetic and environmental factors on the noise-induced hearing loss (NIHL) are still unclear. This study aimed to assess interactions among gene polymorphisms, noise metrics, and lifestyles on the risk of NIHL. Methods: A case-control study was conducted using 307 patients with NIHL and 307 matched healthy individuals from five manufacturing industries. General demographic data, lifestyle details, and noise exposure levels were recorded. The Kompetitive allele-specific polymerase chain reaction (KASP) was used to analyze the genotypes of 18 SNPs. Results: GMDR model demonstrated a relevant interaction between NRN1 rs3805789 and CAT rs7943316 (P = 0.0107). Subjects with T allele of rs3805789 or T allele of rs7943316 had higher risks of NIHL than those with the SNP pair of rs3805789-CC and rs7943316-AA (P < 0.05). There was an interaction among rs3805789, rs7943316, and kurtosis (P = 0.0010). Subjects exposed to complex noise and carrying both rs3805789-CT and rs7943316-TT or rs3805789-CT/TT and rs7943316-AA had higher risks of NIHL than those exposed to steady noise and carrying both rs3805789-CC and rs7943316-AA (P < 0.05). The best six-locus model involving NRN1 rs3805789, CAT rs7943316, smoking, video volume, physical exercise, and working pressure for the risk of NIHL was found to be the interaction (P = 0.0010). An interaction was also found among smoking, video volume, physical exercise, working pressure, and kurtosis (P = 0.0107). Conclusion: Concurrence of NRN1 and CAT constitutes a genetic risk factor for NIHL. Complex noise exposure significantly increases the risk of NIHL in subjects with a high genetic risk score. Interactions between genes and lifestyles as well as noise metrics and lifestyles affect the risk of NIHL.

18.
Rapid Commun Mass Spectrom ; : e9174, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34350664

RESUMO

RATIONALE: Kaixin San (KXS) is a prescription medicine of traditional Chinese medicine (TCM) with the effects of "tonifying the kidney and brain" and "improving memory". The volatile organic compounds (VOCs) in KXS could effectively improve senile dementia and depression, but rare studies focused on the overall characterization of VOCs in KXS and the quantitative study of the main active components. METHODS: In this paper, a strategy to correlate the results from headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace-gas chromatography-mass spectrometry (HS-GC-MS) method was developed for comprehensive characterization of VOCs in KXS and quantitative analysis of the main pharmacodynamic substances. RESULTS: 68 small molecular VOCs were identified in KXS by HS-GC-IMS at room temperature and atmospheric pressure; 117 VOCs were identified and 10 components (isocalamenediol, α-asarone, ß-asarone, methyl eugenol, isoeugenol methyl ether, camphor, anethol, 2,4-di-tert-butylphol, linalool, asarylaldehyde) as the quality markers of KXS based on HS-GC-MS. CONCLUSIONS: This research provides a foundation for quality control, pharmacodynamic mechanism research and further development of KXS, and gives more convincing data supporting the VOCs of other natural product.

19.
Adv Mater ; : e2103000, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34397123

RESUMO

The competing and non-equilibrium phase transitions, involving dynamic tunability of cooperative electronic and magnetic states in strongly correlated materials, show great promise in quantum sensing and information technology. To date, the stabilization of transient states is still in the preliminary stage, particularly with respect to molecular electronic solids. Here, a dynamic and cooperative phase in potassium-7,7,8,8-tetracyanoquinodimethane (K-TCNQ) with the control of pulsed electromagnetic excitation is demonstrated. Simultaneous dynamic and coherent lattice perturbation with 8 ns pulsed laser (532 nm, 15 MW cm-2 , 10 Hz) in such a molecular electronic crystal initiates a stable long-lived (over 400 days) conducting paramagnetic state (≈42 Ωcm), showing the charge-spin bistability over a broad temperature range from 2 to 360 K. Comprehensive noise spectroscopy, in situ high-pressure measurements, electron spin resonance (ESR), theoretical model, and scanning tunneling microscopy/spectroscopy (STM/STS) studies provide further evidence that such an transition is cooperative, requiring a dedicate charge-spin-lattice decoupling to activate and subsequently stabilize nonequilibrium phase. The cooperativity triggered by ultrahigh-strain-rate (above 106 s- 1 ) pulsed excitation offers a collective control toward the generation and stabilization of strongly correlated electronic and magnetic orders in molecular electronic solids and offers unique electro-magnetic phases with technological promises.

20.
Plant Dis ; 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34384251

RESUMO

Pectobacterium species cause blackleg, soft rot and stem rot in potato and many other vegetable crops (Charkowski 2015). In July 2020, potato plants showing characteristic symptoms of aerial stem rot were observed in a field (cv. Xisen 6) in Fengning Manchu Autonomous County, Chengde, Hebei Province (North China). The disease incidence in that field (5 ha in size) was more than 50%. Putative pectolytic bacteria were obtained from symptomatic stem tissues (light brown and water-soaked stem sections) by culturing on the crystal violet pectate (CVP) medium. Bacterial colonies producing pits, were restreaked and purified on Luria-Bertani (LB) agar. The isolates causing stem rot were gram negative and rod shaped, negative for oxidase, urease, indole production, gelatin liquefaction and acid production from maltose and D-sorbitol. All isolates were catalase positive, produced acid from lactose, rhamnose, saccharose, raffinose and D-arabinose, and were tolerant to 5% NaCl, and able to utilize citrate. The bacterial gDNA was extracted using the EasyPure Bacteria Genomic DNA Kit (TransGen Biotech). The 16S rDNA region was amplified by PCR using the universal primer pair 27F/1492R and sequenced. Result of the Blastn analysis of the 16S rDNA amplicons (MZ379788, MZ379789) suggested that the isolates FN20111 and FN20121 belonged to the genus Pectobacterium. To determine the species of the stem rot Pectobacterium isolates, multi-locus sequence analysis (MLSA) was performed with six housekeeping genes acnA, gapA, icdA, mdh, proA and rpoS (MZ403781-MZ403792), and phylogenetic tree was reconstructed using RAxML v8.2.12 (https://github.com/stamatak/standard-RAxML). The result of phylogenetic analysis showed that the stem rot Pectobacterium isolates FN20111 and FN20121 clustered with P. versatile (syn. 'Candidatus Pectobacterium maceratum') strains CFBP6051T (Portier et al. 2019), SCC1 (Niemi et al. 2017) and F131 (Shirshikov et al. 2018). And the isolates FN20111 and FN20121 were more closely related to the type strain CFBP6051T than to strains SCC1 and F131. Potato seedlings (cv. Xisen 6 and Favorita) were inoculated with the isolates FN20111 and FN20121 by injecting 100 µl of bacterial suspensions (108 CFU·mL-1) into the upper parts of the stems of potato plants, or injected with 100 µl of 0.9% saline solution as control. The seedlings were grown at 28°C and 50% relative humidity. Three days post-inoculation, only the bacteria-inoculated seedlings showed diseased symptoms resembling to those observed in the field. Bacterial colonies were obtained from the infected stems and were identified using the same PCR primers of housekeeping genes as described above, fulfill Koch's postulates. P. versatile causing soft rot and blackleg on potato plants has been reported in Finland (Niemi et al. 2017), Russia (Shirshikov et al. 2018), Netherlands (Portier et al. 2019), Poland (Waleron et al. 2019) and in New York State (Ma et al. 2021). To our knowledge, this is the first report of P. versatile causing aerial stem rot of potato in China.

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