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1.
J Dermatol Sci ; 2022 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-36075780

RESUMO

BACKGROUND: Lysosomal cell death is induced by lysosomal membrane permeabilization (LMP) and the subsequent release of lysosomal proteolytic enzymes, including cathepsins (CTSs), which results in mitochondrial dysfunction and apoptosis. Imiquimod (IMQ), a synthetic TLR7 ligand, has both antiviral and antitumor activity against various skin malignancies in clinical treatment. Previously, we demonstrated IMQ not only caused lysosomal dysfunction but also triggered lysosome biogenesis to achieve lysosomal adaptation in cancer cells. OBJECTIVE: To determine whether lysosomes are involved in IMQ-induced apoptosis. METHODS: The human skin cancer cell lines BCC, A375 and mouse melanoma cell line B16F10 were used in all experiments. Cell death was determined by the Cell Counting Kit-8 (CCK-8) assay and DNA content assay. Protein expression was determined by immunoblotting. Caspase-8 activity was assessed using a fluorescence caspase-8 kit and determined by flow cytometry and confocal microscopy. RESULTS: IMQ not only induced lysosome damage but also abrogated lysosome function in skin cancer cells. IMQ-induced caspase-8 activation contributed to the processes of lysosomal cell death. Moreover, the use of ROS scavengers significantly abolished caspase-8 activation and inhibited IMQ-induced LMP. Additionally, pharmacological inhibition of CTSD not only abrogated caspase-8 activation but also rescued IMQ-induced cell death. Finally, lysosome-alkalizing agents enhanced the cytotoxicity of IMQ in vitro and in vivo. CONCLUSIONS: IMQ-induced ROS accumulation promotes LMP, releases CTSs into the cytosol, stimulates caspase-8 activation and finally causes lysosomal cell death. Lysosomal cell death and the CTSD/caspase-8 axis may play a crucial role in IMQ-induced cell death.

2.
Pest Manag Sci ; 2022 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-36054636

RESUMO

BACKGROUND: Chlorsulfuron, metsulfuron-methyl and ethametsulfuron can damage sensitive crops in rotation pattern as a result of their long persistence in soil. To explore novel sulfonylurea (SU) herbicides with favorable soil degradation rates, four series of SUs were synthesized through a structure-based drug design (SBDD) strategy. RESULTS: The target compounds, especially Ia, Id and Ie, exhibited prospective herbicidal activity against dicotyledon oil seed rape (Brassica campestris), amaranth (Amaranthus retroflexus), monocotyledon barnyard grass (Echinochloa crusgalli) and crab grass (Digitaria sanguinalis) at a concentration of 15 a.i. g ha-1 . Additionally, Ia, Id and Ig displayed excellent inhibitory effects against AtAHAS, with Kapp i values of 59.1, 34.5 and 71.8 µm, respectively, which were much lower than that of chlorsulfuron at 149.4 µm. The π-π stack and H-bonds between the Ia conformation and AtAHAS in the molecular docking results confirmed the series of compounds to be conventional AHAS inhibitors. In alkaline soil (pH = 8.46), compounds Ia-Ig revealed various degrees of acceleration in the degradation rate compared with chlorsulfuron. Besides, compound Ia showed considerable wheat and corn safety under postemergence at the concentration of 30, 60 and even 120 a.i. g ha-1 . CONCLUSION: Overall, based on the synthetic procedure, herbicidal activity, soil degradation and crop safety, the Ia sulfonylureas series were chosen to be investigated as prospective AHAS inhibitors. The 5-dimethylamino group on SUs accelerated the degradation rate at different levels in alkaline soils which seems to be controllable in conventional cropping systems in their further application. © 2022 Society of Chemical Industry.

3.
ACS Nano ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36094880

RESUMO

Heterostructures have attracted enormous interest due to the properties arising from the coupling and synergizing between multiscale structures and the promising applications in electronics, mechanics, and optics. However, it is challenging for current technologies to precisely integrate cross-scale micro/nanomaterials in three dimensions (3D). Herein, we realize the precise spatial allocation of nanoblocks on micromatrices and programmable 3D optical heterostructure patterning via printing-assisted self-assembly. This bottom-up approach fully exploits the advantages of printing in on-demand patterning, low cost, and mass production, as well as the merits of solution-based colloidal assembly for simple structuring and high-precision regulating, which facilitates the patterned integration of multiscale materials. Importantly, the luminescent nanoparticle assembly can be accurately coupled to the dye-doped polymer matrix by regulating the interface wettability, enabling facile multicolor tuning in a single heterostructure. Thus, the heterostructure can be specially encoded for anticounterfeiting and encryption applications due to the morphology-dependent and interface-coupling-induced luminescence. Moreover, with the capability to achieve single-nanoparticle resolution, these findings have great potential for designing photonic superstructures and advanced optical devices.

4.
Adv Mater ; : e2205284, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36085249

RESUMO

Long cycling lifespan is a prerequisite for practical utilization of lithium-sulfur batteries yet is restricted by the side reactions between soluble polysulfides and lithium metal anodes. The regulation on solvation structure of polysulfides renders encapsulating-polysulfides electrolyte (EPSE) that emerges as a promising solution to suppress the parasitic reactions. The solvating power of the solvents in the outer solvent shell of lithium polysulfides is critical for the encapsulation effect of EPSE. Herein a typical hydrofluoroether, 1,1,2,2-tetrafluoroethyl-2,2,3,3-tetrafluoropropyl ether (HFE) is demonstrated as a superior outer-shell solvent to construct EPSE. On the basis of the large steric hindrance of fluorohydrocarbon chains, the electron-withdrawing -CF2 - segment further endows HFE molecule with prominently weak solvating power. The HFE-EPSE improves the lifespan from 54 to 135 cycles of lithium-sulfur batteries with ultrathin lithium metal anodes (50 µm) and high-areal-loading sulfur cathodes (4.4 mg cm-2 ). Furthermore, a 334 Wh kg-1 lithium-sulfur pouch cell (2.4 Ah level) with HFE-EPSE stably undergoes 25 cycles. This work demonstrates the vital role of weakening solvating power of the outer-shell solvent of lithium polysulfides to construct superior EPSE and inspires the significance of regulating the solvation chemistry of polysulfides to achieve long cycling lifespan of working lithium-sulfur batteries. This article is protected by copyright. All rights reserved.

5.
Neuroimage Clin ; 36: 103198, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36116163

RESUMO

Functional deficits in schizophrenia (SZ) are observed prior to the onset of psychosis and differ at different stages of SZ. However, there is a paucity of studies focused on adolescent first-episode SZ (AOS), adult first-episode SZ (AFES), and adult chronic SZ (CHSZ). In this study, we investigated regional activity and corresponding functional connectivity alterations that have aimed to compare the three disease stages simultaneously. The subjects comprised 49 patients with AOS, 57 patients with AFES, 51 patients with CHSZ, 41 adolescent healthy controls, and 138 adult healthy controls. We compared regional homogeneity (ReHo) between patients at each disease stage with matched healthy controls. We focused on the shared brain regions that showed significant differences between SZ patients at the three different disease stages and healthy controls. Further analysis was conducted to explore whether the patterns of the whole brain functional connectivity alterations were similar. The putamen and medial frontal gyrus (MFG) showed consistently abnormal patterns in AOS, AFES, and CHSZ. Commonly decreased ReHo values in the MFG and increased ReHo values in the bilateral putamen were found in AOS, AFES, and CHSZ. Functional connectivity of MFG remained common abnormality in different SZ stage. In conclusion, ReHo abnormalities in the MFG and the putamen may be common abnormal patterns of brain function in the three different stages of SZ. The vmPFC-dlPFC FC abnormality common occurs in adolescence and adulthood.. This study may provide a more comprehensive understanding of the neurodevelopmental abnormality across the AOS, AFES, and CHSZ.

6.
Sensors (Basel) ; 22(17)2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36080812

RESUMO

The 3D electrode silicon detector eliminates the limit of chip thickness, so it can reduce the electrode spacing (small area) and effectively improve the radiation hardness. In order to expand the application range of the 3D electrode detector, we first propose a 3D large-area silicon detector with a large sensitive volume, and realize multiple floating rings on the upper and lower surfaces of the detector. Due to the influence of different charge states and energy levels in the Si-SiO2 interface system, the top and bottom of the 3D P+ electrode are more prone to avalanche breakdown in the 3D large-area detector before the detector is completely depleted or the carrier saturation drift velocity is reached. Moreover, the electric field distribution becomes very uneven under the influence of the oxide charge, resulting in non-equilibrium carriers that cannot drift in the optimal path parallel to the detector surface. In this paper, the effect of floating rings on the performance of a 3D large-area silicon detector is studied by TCAD simulation. It can increase avalanche breakdown voltage by 14 times in a non-irradiated environment, and can work safely in a moderate irradiated environment. The charge collection efficiency can be effectively improved by optimizing the drift path.

7.
Clin Chem Lab Med ; 2022 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-36113983

RESUMO

OBJECTIVES: Hepatocellular carcinoma (HCC) is difficult to diagnose early and progresses rapidly, making it one of the most deadly malignancies worldwide. This study aimed to evaluate whether salivary glycopattern changes combined with machine learning algorithms could help in the accurate diagnosis of HCC. METHODS: Firstly, we detected the alteration of salivary glycopatterns by lectin microarrays in 118 saliva samples. Subsequently, we constructed diagnostic models for hepatic cirrhosis (HC) and HCC using three machine learning algorithms: Least Absolute Shrinkage and Selector Operation, Support Vector Machine (SVM), and Random Forest (RF). Finally, the performance of the diagnostic models was assessed in an independent validation cohort of 85 saliva samples by a series of evaluation metrics, including area under the receiver operator curve (AUC), accuracy, specificity, and sensitivity. RESULTS: We identified alterations in the expression levels of salivary glycopatterns in patients with HC and HCC. The results revealed that the glycopatterns recognized by 22 lectins showed significant differences in the saliva of HC and HCC patients and healthy volunteers. In addition, after Boruta feature selection, the best predictive performance was obtained with the RF algorithm for the construction of models for HC and HCC. The AUCs of the RF-HC model and RF-HCC model in the validation cohort were 0.857 (95% confidence interval [CI]: 0.780-0.935) and 0.886 (95% CI: 0.814-0.957), respectively. CONCLUSIONS: Detecting alterations in salivary protein glycopatterns with lectin microarrays combined with machine learning algorithms could be an effective strategy for diagnosing HCC in the future.

8.
Exp Brain Res ; 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36114835

RESUMO

Postoperative cognitive dysfunction (POCD) is characterized by cognitive impairments following anesthesia/surgery, but the role of obesity and the underlying mechanisms are not known. We investigated the impact of obesity on POCD. Eighty male C57BL/6 J mice were assigned randomly to two groups fed a normal chow diet (ND, n = 40) or a high-fat diet (HD, n = 40) for 20 weeks. Then, they were divided randomly into eight subgroups of 10: ND control (NDC), ND with surgery (NDS), HD control (HDC), HD with surgery (HDS); NDS + DMSO (NDS + DS), NDS + SRT1720 (NDS + SRT), HDS + DMSO (HDS + DS), and HDS + SRT1720 (HDS + SRT). Body weight, blood glucose level, and serum lipid levels were measured. Staining methods on liver tissues were used to determine hepatic steatosis. A POCD model was established by sleeve gastrectomy (SG) under isoflurane anesthesia. Cognitive function was assessed using the Morris water maze test (MWMT). Expression of sirtuin1 (SIRT1), phosphorylated cAMP-responsive element binding protein (p-CREB), CREB and brain-derived neurotrophic factor (BDNF) in the hippocampus were measured. High-fat diet-fed mice for 20 weeks could establish an obesity model with hyperlipidemia and hepatic steatosis. Cognitive impairment was significantly worse in the HDC and HDS groups than that in the NDC and NDS groups, respectively. Hippocampal expression of SIRT1, p-CREB, and BDNF in the HDS group was significantly lower than that of the HDC group. SRT1720 (SIRT1 activator) pretreatment could attenuate cognitive impairment by upregulating SIRT1 expression. These data suggest that obesity exacerbated postoperative hippocampal-dependent cognitive impairment via a SIRT1 pathway, and SRT1720 pretreatment could alleviate it.

9.
Small ; : e2203207, 2022 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-36057991

RESUMO

Triple ionic and electronic conductivity (TIEC) in cathode materials for protonic ceramic fuel cells (PCFCs) is a desirable feature that enhances the spatial expansion of active reaction sites for electrochemical oxygen reduction reaction. The realization of optimal TIEC in single-phase materials, however, is challenging. A facile route that facilitates the optimization of TIEC in PCFC cathodes is the strategic development of multiphase cathode materials. In this study, a cubic-rhombohedral TIEC nanocomposite material with the composition Ba(CeCo)0.4 (FeZr)0.1 O3- δ (BCCFZ) is designed via self-assembly engineering. The material consists of a mixed ionic and electronic conducting phase, BaCo1-( x + y + z ) Cex Fey Zrz O3- δ (M-BCCFZ), and a dominant proton-conducting phase, BaCe1-( x + y + z ) Cox Zry Fez O3- δ (H-BCCZF). The dominant cerium-rich H-BCCFZ phase enhances the material's oxygen vacancy concentration and the proton defects formation and transport with a low enthalpy of protonation of -30 ± 9 kJ mol-1 . The area-specific resistance of the BCCFZ symmetrical cell is 0.089 Ω cm2 at 650 °C in 2.5% H2 O-air. The peak power density of the anode-supported single cell based on BCCFZ cathode reaches 1054 mW cm-2 at 650 °C with good operation stability spanning over 500 h at 550 °C. These promote BCCFZ as a befitting cathode material geared toward PCFC commercialization.

10.
Phys Rev Lett ; 129(8): 084301, 2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-36053695

RESUMO

Weyl points-topological monopoles of quantized Berry flux-are predicted to spread to Weyl exceptional rings in the presence of non-Hermiticity. Here, we use a one-dimensional Aubry-Andre-Harper model to construct a Weyl semimetal in a three-dimensional parameter space comprising one reciprocal dimension and two synthetic dimensions. The inclusion of non-Hermiticity in the form of gain and loss produces a synthetic Weyl exceptional ring (SWER). The topology of the SWER is characterized by both its topological charge and non-Hermitian winding numbers. We experimentally observe the SWER and synthetic Fermi arc in a one-dimensional phononic crystal with the non-Hermiticity introduced by active acoustic components. Our findings pave the way for studying the high-dimensional non-Hermitian topological physics in acoustics.

11.
Phytochemistry ; 204: 113421, 2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36055425

RESUMO

Eight undescribed phenylpropanoid-dihydrochalcone hybrids, namely (+)- and (-)-malahupin A, (+)- and (-)-malahupin B, (±)-malahupin C, malahupinosides A and B, 7‴-epi-malahupinoside B, together with two known compounds, phloretin and phlorizin, were isolated from the leaves of the folk medicinal plant Malus hupehensis. Their structures were elucidated by extensive NMR and MS spectroscopic methods, chiral-phase analysis, and ECD calculations. Compounds (+)-malahupin B and malahupinoside B showed weak inhibition activities against the nitric oxide production in liposaccharide-induced murine RAW264.7 macrophages with IC50 values of 36.7 and 27.0 µM, respectively. Compounds (+)- and (-)-malahupin A, (+)- and (-)-malahupin B exhibited significant α-glucosidase inhibitory activity, with IC50 values of 22.5, 19.1, 19.2, and 17.4 µM, respectively. The postulated biosynthetic pathways to these hybrid compounds were proposed. This work represents the first report of the natural phenylpropanoid-dihydrochalcone hybrid compound, and lays foundation for the study on the bioactive principles of the ethnic hypoglycemic medicinal plant.

12.
Aging (Albany NY) ; 14(17): 6993-7002, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36057264

RESUMO

Overdose of acetaminophen (APAP) is currently one of the main causes of hepatoxicity and acute liver injury, which is often linked to oxidative stress. Phellinus linteus polysaccharides (Phps) have shown many hepatoprotective effects, however, the mechanism of Phps on APAP-induced acute liver injury has not been further elucidated. The aim of this study is to investigate the underlying mechanism of Phps to acute liver injury. The expression of AMPK/Nrf2 and autophagy were detected using western blot. The results indicated that Phps treatment effectively alleviated APAP-induced acute liver injury by reducing alanine transaminase (ALT) and aspartate aminotransferase (AST) levels in serum. Phps significantly attenuated myeloperoxidase (MPO) activity and glutathione (GSH) depletion. Meanwhile, Phps remarkably alleviated histopathological changes. Further research found that Phps promoted AMPK pathway and up-regulated nuclear factor erythroid-2-related factor (Nrf2) transported into nucleus, and elevated heme oxygenase 1(HO-1), glutamate-cysteine ligase catalytic (GCLC), glutamate cysteine ligase modifier (GCLM) and quinone oxidoreductase (NQO1). Additionally, Phps apparently facilitated the expression of autophagy proteins (ATG3, ATG5, ATG7, and ATG12). However, the protection of pathologic changes was nearly absent in Nrf2-/- mice. Phps have potential in preventing oxidative stress in APAP-induced acute liver injury.

13.
Int J Biol Macromol ; 221: 986-993, 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36075310

RESUMO

Maleic acid (MA) is an effective solvent for lignin extraction from raw biomass under mild conditions through acidolysis and hydrophobic interaction. To improve the valorization of lignin, the lignin extracted with MA was subjected to successive organic solvent fractionation to make the narrow Mw and Mn distribution and the homogeneity structure of lignin fragments. After successive organic solvent fractionation, the dispersity index (D) of lignin reduced from 2.86 to 1.25, and the purity and homogeneity were greatly improved. The phenolic hydroxyl content of lignin extracted by ethyl acetate was the highest (2.45 mmol/g), and the aliphatic-OH concentration was the lowest (0.65 mmol/g). While the ßO4 bonds were partially cleaved, more hydrophobic substrates with CC linkages were recovered by ethyl acetate. Lignin fragments with a variety of main structures and functional groups were obtained effectively with successive fractionation. This method supplied broad prospects for the preparation of lignin and its applications in epoxy resin, rubber, thermoplastic additives and polymer raw materials.

14.
Environ Pollut ; 313: 120154, 2022 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-36096264

RESUMO

The catalytic hydrogenolysis of a typical model compound of mulching film waste, polyethylene, was investigated as a potential way to improve economic efficiency of mulching film recycling. Nickel-based heterogeneous catalysts are proposed for polyethylene hydrogenolysis to produce liquid hydrocarbons. Among catalysts supported on various carriers, Ni/SiO2 catalyst shows the highest activity which may due to the interactions between nickel and silica with the formation of nickel phyllosilicate. As high as 81.18% total gasoline and diesel range hydrocarbon was obtained from the polyethylene hydrogenolysis at relatively mild condition of 280 °C, and 3 MPa cold hydrogen pressure. The result is comparable to what have been reported in previous studies using noble metal catalysts. The gasoline and diesel range hydrocarbon are n-alkanes with a distribution at a range of C4-C22. The gas products are primarily CH4 along with a small amount of C2H6 and C3H8. High yield of CH4 as much as 9.68% was observed for the cleavage of molecule occurs along the alkane chain.

15.
Front Nutr ; 9: 996811, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091224

RESUMO

Xylo-oligosaccharide (XOS) is a class of functional oligosaccharides that have been demonstrated with prebiotic activity over several decades. XOS has several advantages relative to other oligosaccharide molecules, such as promoting root development as a plant regulator, a sugar supplement for people, and prebiotics to promote intestinal motility utilization health. Now, the preparation and extraction process of XOS is gradually mature, which can maximize the extraction and avoid waste. To fully understand the recent preparation and application of XOS in different areas, we summarized the various technologies for obtaining XOS (including acid hydrolysis, enzymatic hydrolysis, hydrothermal pretreatment, and alkaline extraction) and current applications of XOS, including in animal feed, human food additives, and medicine. It is hoped that this review will serve as an entry point for those looking into the prebiotic field of research, and perhaps begin to dedicate their work toward this exciting classification of bio-based molecules.

16.
Reprod Sci ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36097247

RESUMO

The purpose of this study is to investigate the effect of aerobic exercise (AE) training and/or oyster peptide (OP) supplementation on the formation of late-onset hypogonadism (LOH). AE training and/or OP supplement was performed during Cytoxan (CTX)-induced LOH formation in male SD rats for 6 consecutive weeks. Low dose of CTX could decrease mating times, the levels of luteinizing hormone (LH), total testosterone (TT), free testosterone (FT) in serum and TT, androgen receptor (AR), androgen binding protein (ABP), and glutathione peroxidase (GSH-Px) in testicle, but increase capture latency, mating latency, and malondialdehyde, and downregulate the mRNA expression of steroidogenic acute regulatory (StAR), P450 cholesterol side chain cleavage enzyme (P450scc), and StAR-related lipid transfer domain 7 (StARD7) in testicle. Every change was altered by AE training combined with OP supplement significantly, except for serum LH. Moreover, the effect of AE training combined with OP supplement was better than that of AE training on serum TT, FSH, testicular TT, mating latency, capture times, and mating times. AE training combined with OP supplement during CTX-induced LOH formation can prevent the LOH development by enhancing pituitary-gonads axis's function and reducing testicular oxidative stress to promote testosterone synthesis and spermatogenesis.

17.
Int J Biol Macromol ; 2022 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-36126806

RESUMO

Volatile fatty acids (VFAs) derived from biomass are considered to be economical and environmentally friendly feedstocks for microbial fermentation. Converting VFAs to polyhydroxyalkanoate (PHA) could reduce the substrate cost and provide an economically viable route for the commercialization of PHA. The halophilic bacteria Salinivibrio spp. TGB4 and TGB19, newly isolated from salt fields, were found to accumulate poly-3-hydroxybutyrate (PHB) using acetate or butyrate as the substrate. Both strains exhibited considerable cell growth (OD600 of ~8) even at acetate concentration of 100 g/L. In shake flask cultures, TGB4 produced PHB titers of 0.90 and 1.34 g/L, while TGB19 produced PHB titers of 0.25 and 2.53 g/L with acetate and butyrate, respectively. When acetate and butyrate were both applied, PHB production was significantly increased, and the PHB titer of TGB4 and TGB19 reached 6.14 and 6.84 g/L, respectively. After optimizing the culture medium, TGB19 produced 8.42 g/L PHB, corresponding to 88.55 wt% of cell dry weight. During fed-batch cultivation, TGB19 produced a PHB titer of 53.23 g/L. This is the highest reported PHB titer using acetate and butyrate by pure microbial cultures and would provide promising hosts for the industrial production of PHA from VFAs.

18.
Front Microbiol ; 13: 922444, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118220

RESUMO

A total of eleven new dimeric chromanones, paecilins F-P (2-12), were isolated from the endophytic fungus Xylaria curta E10, along with four known analogs (1, 13-15). Their structures and absolute configurations were determined by extensive experimental spectroscopic methods, single-crystal X-ray diffraction, and equivalent circulating density (ECD) calculations. In addition, the structure of paecilin A, which was reported to be a symmetric C8-C8' dimeric pattern, was revised by analysis of the nuclear magnetic resonance (NMR) data, and single-crystal X-ray diffraction. Compound 1 showed antifungal activity against the human pathogenic fungus Candida albicans with a minimum inhibitory concentration of 16 µg/mL, and Compounds 8 and 10 showed antibacterial activity against the gram-negative bacterium Escherichia coli with the same minimum inhibitory concentration of 16 µg/mL.

19.
Zootaxa ; 5162(4): 397-409, 2022 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-36095500

RESUMO

To date, only two whip spider species have been recorded in China. We describe a new species, Sarax sinensis sp. nov., from Fujian, China. This species is morphologically similar to S. ioanniticus (Kritscher, 1959), S. israelensis (Miranda et al., 2016), and S. seychellarum (Kraepelin, 1898), but can be distinguished by the combination of the following characters: 35 segments in leg I tarsus, eight teeth on cheliceral claw, and four dorsal and ventral spines respectively on pedipalp femur. To examine the evolutionary history of S. sinensis sp. nov., we sequenced 12S, 16S, and COI gene regions of our specimens and inferred its phylogenetic position. The inferred phylogenetic trees placed the new species within Sarax, with its closest relative being distributed across the western Asia. The type specimens are deposited in the Museum of Biology, East China Normal University (ECNU).


Assuntos
Aracnídeos , Aranhas , Distribuição Animal , Animais , Tamanho Corporal , China , Humanos , Tamanho do Órgão , Filogenia , Aranhas/genética
20.
Cell Death Dis ; 13(9): 772, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071042

RESUMO

Lung adenocarcinoma (LUAD) is one of the main causes of cancer-related mortality, with a strong tendency to metastasize early. Transforming growth factor-ß (TGF-ß) signaling is a powerful regulator to promote metastasis of LUAD. Here, we screened long non-coding RNAs (lncRNAs) responsive to TGF-ß and highly expressed in LUAD cells, and finally obtained our master molecular LINC00152. We proved that the TGF-ß promoted transcription of LINC00152 through the classical TGF-ß/SMAD3 signaling pathway and maintained its stability through the RNA-binding protein HuR. Moreover, LINC00152 increased ZEB1, SNAI1 and SNAI2 expression via increasing the interactions of HuR and these transcription factors, ultimately promoting epithelial-mesenchymal transition of LUAD cell and enhancing LUAD metastasis in vivo. These data provided evidence that LINC00152 induced by TGF-ß promotes metastasis depending HuR in lung adenocarcinoma. Designing targeting LINC00152 and HuR inhibitors may therefore be an effective therapeutic strategy for LUAD treatment.


Assuntos
Adenocarcinoma de Pulmão , Proteína Semelhante a ELAV 1 , Neoplasias Pulmonares , RNA Longo não Codificante , Fator de Crescimento Transformador beta , Adenocarcinoma de Pulmão/patologia , Linhagem Celular Tumoral , Proteína Semelhante a ELAV 1/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Pulmonares/patologia , RNA Longo não Codificante/genética , Fator de Crescimento Transformador beta/farmacologia
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