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1.
Clin Cancer Res ; 2020 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-32234760

RESUMO

PURPOSE: Adults with T-cell lymphoblastic lymphoma (T-LBL) generally benefit from treatment with acute lymphoblastic leukemia (ALL)-like regimens, but approximately 40% will relapse after such treatment. We evaluated the value of CpG methylation in predicting relapse for adults with T-LBL treated with ALL-like regimens. EXPERIMENTAL DESIGN: A total of 549 adults with T-LBL from 27 medical centers were included in the analysis. Using the Illumina Methylation 850K Beadchip, 44 relapse-related CpGs were identified from 49 T-LBL samples by two algorithms: least absolute shrinkage and selector operation (LASSO) and support vector machine-recursive feature elimination (SVM-RFE). We built a four-CpG classifier using LASSO Cox regression based on association between the methylation level of CpGs and relapse-free survival in the training cohort (n = 160). The four-CpG classifier was validated in the internal testing cohort (n = 68) and independent validation cohort (n = 321). RESULTS: The four-CpG-based classifier discriminated patients with T-LBL at high risk of relapse in the training cohort from those at low risk (P < 0.001). This classifier also showed good predictive value in the internal testing cohort (P < 0.001) and the independent validation cohort (P < 0.001). A nomogram incorporating five independent prognostic factors including the CpG-based classifier, lactate dehydrogenase levels, Eastern Cooperative Oncology Group performance status, central nervous system involvement, and NOTCH1/FBXW7 status showed a significantly higher predictive accuracy than each single variable. Stratification into different subgroups by the nomogram helped identify the subset of patients who most benefited from more intensive chemotherapy and/or sequential hematopoietic stem cell transplantation. CONCLUSIONS: Our four-CpG-based classifier could predict disease relapse in patients with T-LBL, and could be used to guide treatment decision.

2.
Water Res ; 178: 115834, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32339865

RESUMO

Bioactivity and settleability of activated sludge are essential for the operation of activated sludge systems in wastewater treatment. In this work, the fractal dimension of sludge image is proposed as a tool to evaluate these two factors. The specific endogenous respiration rate (SOURe) and the specific quasi-endogenous respiration rate (SOURq) are found to be more dependent on the 3D structure of sludge than the specific total respiration rate (SOURt). The relationship between the fractal structure and bioactivity suggests that the bioactivity governs the acceptable upper bound of the fractal dimension (Df), as at its theoretical maximum of 2.0, the non-porous compact flocs are predominant. The settleability or the biomass concentration determines the acceptable lower bound of Df, as at its theoretical minimum of 1.0, the free-swimming microbes are predominant. Our data reveal that the activated sludge has an acceptable fractal dimension Df in a range of 1.07-1.68. In practice, the fractal dimension should be controlled at a reasonable value as there is a trade-off between the bioactivity and physical structure to achieve better performance. A decrease or increase in the fractal dimension can serve as a signal for the change of the operational status, and this is further elucidated from the perspective of settling tanks using state point analysis. Compared with respirogram measurement, measuring fractal dimension is a complex process and its online implementation is challenging. Also, the measured value varies with the methods used. In addition, the difference in their theoretical values depends on the homogeneity of the sludge structure. Since the fractal dimension Df reflects both bioactivity and settleability of the sludge but is difficult to measure, in this work a relationship between Df and the easily measurable respirogram is established, and a method using the respirogram as a proxy of Df is proposed to control the bioactivity and settleability simultaneously. This respiration-based method is able to simultaneously control aeration and settling tanks, and could serve as an efficient tool for the management of wastewater treatment plants.

3.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(1): 69-74, 2020 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-32037769

RESUMO

OBJECTIVE: To assess the efficacy of bone anchorage and maxillary facemask protraction devices in treating skeletal class Ⅲ malocclusion in adolescents. METHODS: Articles relating to the use of bone anchorage and maxillary facemask protraction devices for treating skeletal class Ⅲ malocclusion in adolescents were searched from the databases of Cochrane Library, PubMed, EmBase, CNKI, and Wanfang database. Several inclusion and exclusion criteria were developed for the article screening. The clinical data were extracted, and the quality of the selected articles was evaluated. A Meta-analysis of SNA, SNB, ANB, ANS-Me, Wits, and U1-PP change was performed by using RevMan 5.3. RESULTS: Seven studies (264 patients) were included in the Meta-analysis. Among these studies, three were randomized controlled trials, and four were non-randomized controlled trials. Compared with the maxillary facemask protraction device group, the bone ancho-rage device group had higher SNA changes and lower ANS-Me, Wits, and U1-PP changes (P<0.05). No significant differences were observed in the SNB and ANB changes between these two groups. CONCLUSIONS: Compared with the maxillary facemask protraction device, the bone anchorage device can increase the extent of protraction of the maxilla and has better controls for the labial inclination of the maxillary anterior teeth in treating skeletal class Ⅲ malocclusion among adolescents. However, additional high-quality randomized controlled trials must be performed to verify the results.


Assuntos
Má Oclusão de Angle Classe III , Maxila , Adolescente , Cefalometria , Aparelhos de Tração Extrabucal , Humanos , Técnica de Expansão Palatina , Ensaios Clínicos Controlados Aleatórios como Assunto
4.
Leukemia ; 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32080345

RESUMO

We aimed to establish a discriminative gene-expression-based classifier to predict survival outcomes of T-cell lymphoblastic lymphoma (T-LBL) patients. After exploring global gene-expression profiles of progressive (n = 22) vs. progression-free (n = 28) T-LBL patients, 43 differentially expressed mRNAs were identified. Then an eleven-gene-based classifier was established using LASSO Cox regression based on NanoString quantification. In the training cohort (n = 169), high-risk patients stratified using the classifier had significantly lower progression-free survival (PFS: hazards ratio 4.123, 95% CI 2.565-6.628; p < 0.001), disease-free survival (DFS: HR 3.148, 95% CI 1.857-5.339; p < 0.001), and overall survival (OS: HR 3.790, 95% CI 2.237-6.423; p < 0.001) compared with low-risk patients. The prognostic accuracy of the classifier was validated in the internal testing (n = 84) and independent validation cohorts (n = 360). A prognostic nomogram consisting of five independent variables including the classifier, lactate dehydrogenase levels, ECOG-PS, central nervous system involvement, and NOTCH1/FBXW7 status showed significantly greater prognostic accuracy than each single variable alone. The addition of a five-miRNA-based signature further enhanced the accuracy of this nomogram. Furthermore, patients with a nomogram score ≥154.2 significantly benefited from the BFM protocol. In conclusion, our nomogram comprising the 11-gene-based classifier may make contributions to individual prognosis prediction and treatment decision-making.

5.
Stem Cell Rev Rep ; 16(2): 344-356, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31927699

RESUMO

Stem cell transplantation has been limited by poor survival of the engrafted cells in hostile microenvironment of the infarcted myocardium. This study investigated cytoprotective effect of rapamycin-preactivated autophagy on survival of the transplanted mesemchymal stem cells (MSCs). MSCs isolated from rat bone marrow were treated with 50 nmol/L rapamycin for 2 h, and then the cytoprotective effect of rapamycin was examined. After intramyocardial transplantation in rat ischemia/reperfusion models, the survival and differentiation of the rapamycin-pretreated calls were accessed. After treatment with rapamycin, autophagic activities and lysososme production of the cells were increased significantly. In the condition of short-term or long-term hypoxia and serum deprivation, the apoptotic cells in rapamycin-pretreated cells were less, and secretion of HGF, IGF-1, SCF, SDF-1 and VEGF was increased. After transplantation of rapamycin-pretreated cells, repair of the infarcted myocardium and restoration of cardial function were enhanced dramatically. Expression of HGF, IGF-1, SCF, SDF-1, VEGF, HIF-1α and IL-10 in the myocardium was upregulated, while expression of IL-1ß and TNF-α was downregulated. Tracing of GFP and Sry gene showed that the survival of rapamycin-pretreated cells was increased. Cardiomyogenesis and angiogenesis in the infarcted myocardium were strengthened. Some rapamycin-pretreated cells differentiated into cardiomyocytes or endothelial cells. These results demonstrate that moderate preactivation of autophagy with rapamycin enhances the survival and differentiation of the transplanted MSCs. Rapamycin-primed MSCs can promote repair of the infarcted myocardium and improvement of cardiac function effectively.

6.
Environ Pollut ; 259: 113868, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31887590

RESUMO

Although the coexistence of heavy metals and environmental hormones always occur in aquatic environment, the information of the combined impacts remains unclear. To explore the multi-generational toxicity of cadmium (Cd) and tributyltin (TBT), adult zebrafish (Danio rerio) (F0) were exposed to different treated groups (100 ng/l Cd, 100 ng/l TBT and their mixture) for 90 d, with their offspring (F1 and F2) subsequently reared in the same exposure solutions corresponding to their parents. Both developmental neurotoxicity and thyroid disturbances were examined in the three (F0, F1, and F2) generations. Our results showed that co-exposure to Cd and TBT induced the developmental neurotoxicity in F1 and F2 generations, reflected by the significant lower levels of neurotransmitters (dopamine and serotonin) and the inhibited acetylcholinesterase (AChE) activities. And the thyroid endocrine disruption were observed in the two-generations larval offspring by parental exposure to Cd and/or TBT, including the significantly decreasing levels of thyroid hormones and the down-regulated the expression of genes involved in the hypothalamus-pituitary-thyroid axis, compared to the control. Additional, the embryonic toxicity and growth inhibition were also determined in the fish larvae. Overall, this study examined the impacts of parental co-exposure to Cd and TBT, with regard to developmental inhibition, nervous system damage and endocrine disruption, which highlighted that co-exposure influences are complicated and need to be considered for accurate environmental risk assessment.


Assuntos
Cádmio , Glândula Tireoide , Compostos de Trialquitina , Poluentes Químicos da Água , Peixe-Zebra , Animais , Cádmio/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Disruptores Endócrinos/toxicidade , Glândula Tireoide/efeitos dos fármacos , Compostos de Trialquitina/toxicidade , Poluentes Químicos da Água/toxicidade
7.
Chemosphere ; 240: 124936, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31568941

RESUMO

Triphenyltin (TPT) is widely used and commonly found in a water environment, so its effects on aquatic systems are of great concern. This study aimed to reveal the effects of chronic parental exposure of TPT on thyroid disruption and growth inhibition in zebrafish. Adult zebrafish (F0 generation) were exposed to environmentally relevant concentrations (1, 10, and 100 ng/L) of TPT for 60 days, and the larvae (F1 generation) were tested without TPT treatment. Results demonstrated that parental exposure to TPT disrupts thyroid function in zebrafish offspring: serum thyroxine (T4) significantly decreased, while serum 3,5,3'-triiodothyronine (T3) increased, and several genes involved in the hypothalamic-pituitary-thyroid (HPT) axis were down-regulated. In addition, we observed developmental abnormalities in the larvae, demonstrated by a significantly altered hatching rate, malformation rate, body length, heart rate, and survival rate, as well as down-regulation of genes involved in the growth hormone/insulin-like growth factor (GH/IGF) axis. Therefore, parental exposure to TPT induces toxicity in fish offspring through perturbation of the HPT and GH/IGF axes.


Assuntos
Larva/crescimento & desenvolvimento , Compostos Orgânicos de Estanho/toxicidade , Praguicidas/toxicidade , Glândula Tireoide/patologia , Poluentes Químicos da Água/toxicidade , Animais , Feminino , Hormônio do Crescimento/genética , Hormônio do Crescimento/metabolismo , Larva/efeitos dos fármacos , Masculino , Exposição Materna/efeitos adversos , Exposição Paterna/efeitos adversos , Somatomedinas/genética , Somatomedinas/metabolismo , Glândula Tireoide/efeitos dos fármacos , Tiroxina/sangue , Tri-Iodotironina/sangue , Peixe-Zebra/embriologia
8.
Huan Jing Ke Xue ; 40(6): 2813-2820, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854675

RESUMO

Physical properties and bioactivity are two important aspects of the activated sludge process. To solve the contradiction between these two aspects in the operation control process, the physical structure and respiration processes of sludge in different activated sludge systems were analyzed. Sludge from seven different activated sludge systems was used for the experiments, and the particle size, microscopic morphology, fractal dimension, compression settling performance, adsorption performance, and respirogram were studied and analyzed. The results showed that the correlations between physical performance indicators and respiration rate were very good. Sludge particle size (d) was negatively correlated with the endogenous specific respiration rate (SOURe) and maximum specific respiration rate (SOURt) (R2>0.9); fractal dimension (Df) was linearly and negatively correlated with SOURe and SOURt (R2>0.8); sludge compression index (SCI) and sludge volume index (SVI) were positively correlated with SOURe (R2>0.9); equilibrium adsorption (Qmax) was linearly and negatively correlated with the quasi-endogenous specific respiration rate (SOURq) and SOURe (R2>0.9). In addition, the optimal physical properties and bioactivity range of activated sludge under normal operating conditions were obtained as follows:SVI of 50-120 mL·g-1, SOURe of 6.27-7.55 mg·(g·h)-1, d of 205.80-228.12 µm, Df of 1.56-1.60, Rn/t of 0.02-0.03, and Qmax of 508-636 mg·g-1.

9.
Environ Sci Pollut Res Int ; 26(35): 36048-36054, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31745787

RESUMO

The essential oils (EOs) from aerial parts of Saussurea amara (L.) DC. (SAEO) and Sigesbeckia pubescens Makino (SPEO) were analyzed for their chemical composition by GC-MS, and their repellent activities against adults of the red flour beetle, Tribolium castaneum Herbst, as well as the booklouse, Liposcelis bostrychophila Badonnel, were evaluated for the first time. Results of GC-MS analysis indicated that both SAEO and SPEO were characterized by high content of sesquiterpenoids (relative content > 70%) including oxygenated sesquiterpenoids. The two oil samples and their major component caryophyllene oxide exerted beneficial repellent effects on T. castaneum and L. bostrychophila at 2 and 4 h post-exposure. At 4 h post-exposure, the PR value of caryophyllene oxide could still reach 92% (class V) against T. castaneum at minimum testing concentration of 3.15 nL/cm2, and this compound was observed to result in the greatest repellency (PR = 100%) against L. bostrychophila at 12.63 nL/cm2. This work confirmed the potent repellent efficacy of SAEO and SPEO for controlling pest damage and suggested their potential to be developed into botanical repellents.


Assuntos
Repelentes de Insetos/toxicidade , Óleos Voláteis/toxicidade , Saussurea/fisiologia , Sesquiterpenos/toxicidade , Animais , Asteraceae , Besouros/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Insetos/efeitos dos fármacos , Inseticidas/análise , Óleos Voláteis/química , Controle de Pragas , Tribolium/efeitos dos fármacos
10.
Ecotoxicol Environ Saf ; 186: 109776, 2019 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-31606647

RESUMO

In the present study, to evaluate neurobehavioral toxicity and the thyroid-disrupting effects of environmental levels of triphenyltin (TPT), the zebrafish larvae were exposed to 1, 10 and 100 ng/l TPT. In the neurobehavioral assay, increased levels of dopamine and serotonin, decreased content of nitric oxide, inhibited activities of acetylcholinesterase and monoamine oxidase were observed in the whole body of zebrafish larvae after TPT treatment, as well as the serious abnormal non-reproductive behavior. Moreover, the whole-body the T4 levels were markedly decreased significantly, whereas T3 levels were not significantly changed under TPT stress. In addition, TPT exposure significantly changed the expression levels of genes related to thyroid system, including corticotropin-releasing hormone gene crh, thyroid-stimulating hormone gene tshß, thyroglobulin gene tg, sodium/iodide symporter gene nis, thyroid hormone nuclear receptor trα, isoform trß, types I deiodinase gene dio1and types II deiodinase gene dio2. The regulated responsiveness of thyroid hormone and related genes expression levels suggested that TPT could induce the thyroid disrupting effects in zebrafish larvae. Therefore, our results provide new aspects of TPT as an endocrine disrupting chemical.


Assuntos
Comportamento Animal/efeitos dos fármacos , Disruptores Endócrinos/toxicidade , Compostos Orgânicos de Estanho/toxicidade , Glândula Tireoide/efeitos dos fármacos , Peixe-Zebra/metabolismo , Animais , Hormônio Liberador da Corticotropina/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Iodeto Peroxidase/genética , Larva/efeitos dos fármacos , Larva/metabolismo , Glândula Tireoide/metabolismo , Hormônios Tireóideos/metabolismo , Tireotropina/genética , Proteínas de Peixe-Zebra/metabolismo
11.
Artigo em Inglês | MEDLINE | ID: mdl-31374296

RESUMO

The adverse influences of triphenyltin (TPT) on aquatic system have been of great concern due to their widespread use and ubiquity in water environment. Here, zebrafish larvae (7 days after hatching) were exposed to TPT for 14 days to study its toxicity on the antioxidant system, energy metabolism and the expression of genes related to physiological stress. Results shows that the oxidative stress was generated in fish larvae exposed to TPT with higher concentrations (10 and/or 100 ng/l), and the energy metabolic parameters (RNA/DNA ratio, Na + -K + -ATPase) were significantly inhibited in fish exposed to 100 ng/l TPT. Additional, the expression levels of genes related to physiological stress were up-regulated in a dose-dependent manner, including heat shock protein70 (hsp70) and metallothionein (mt). Moreover, the PERK-eIF2α pathway was found as the main branch activated by TPT exposure in fish larvae. Thus, TPT-induced antioxidant responses, energy metabolism disorder and physiological stress in fish larvae were reflected by the parameters measured, which could provide some useful information for full understanding the exact mechanisms of TPT toxicity.


Assuntos
Larva/metabolismo , Compostos Orgânicos de Estanho/toxicidade , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/metabolismo , Animais , Metabolismo Energético , Fator de Iniciação 2 em Eucariotos/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Metalotioneína/metabolismo , Estresse Oxidativo , eIF-2 Quinase/metabolismo
12.
Leukemia ; 33(10): 2454-2465, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-30953029

RESUMO

New prognostic factors are needed to establish indications for haematopoietic stem cell transplantation (HSCT) in first complete remission (CR1) for T-cell lymphoblastic lymphoma (T-LBL) patients. We used microarray to compare T-LBL tissue samples (n = 75) and fetal thymus tissues (n = 20), and identified 35 differentially expressed miRNAs. Using 107 subjects as the training group, we developed a five-miRNA-based classifier to predict patient survival with LASSO Cox regression: lower risk was associated with better prognosis (disease-free survival (DFS): hazard ratio (HR) 4.548, 95% CI 2.433-8.499, p < 0.001; overall survival (OS): HR 5.030, 95% CI 2.407-10.513, p < 0.001). This classifier displayed good performance in the internal testing set (n = 106) and the independent external set (n = 304). High risk was associated with more favorable response to HSCT (DFS: HR 1.675, 95% CI 1.127-2.488, p = 0.011; OS: HR 1.602, 95% CI 1.055-2.433, p = 0.027). When combined with ECOG-PS and/or NOTCH1/FBXW7 status, this classifier had even better prognostic performance in patients receiving HSCT (DFS: HR 2.088, 95% CI 1.290-3.379, p = 0.003; OS: HR 1.996, 95% CI 1.203-3.311, p = 0.007). The five-miRNA classifier may be a useful prognostic biomarker for T-LBL adults, and could identify subjects who could benefit from HSCT.


Assuntos
MicroRNAs/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Linfócitos T/metabolismo , Linfócitos T/patologia , Intervalo Livre de Doença , Feminino , Transplante de Células-Tronco Hematopoéticas/métodos , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Modelos de Riscos Proporcionais , Indução de Remissão/métodos
13.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(1): 106-108, 2019 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-30854830

RESUMO

Impacted molars are more common in maxillary and mandibular third molars, whereas impacted first molars are relatively rare. A case of horizontal impaction of mandibular first molar is reported in this study, and the relevant literature are presented.


Assuntos
Dente Serotino , Dente Impactado , Humanos , Mandíbula , Maxila , Dente Molar
14.
J Phys Condens Matter ; 31(25): 255601, 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-30870818

RESUMO

We systematically explore the ground state properties of one dimensional fermions with long-range interactions decaying in a power law ∼[Formula: see text] through the density matrix renormalization group algorithm. By comparing values of Luttinger liquid parameters precisely measured in two different ways, we show convincing evidence that Luttinger liquid theory is valid if [Formula: see text] is larger than some threshold, otherwise the theory breaks down. Combining analysis on structure factor, charge gap and charge stiffness, we determine how the metal-insulator transition point develops as the interaction range is continuously tuned. A region in the range of [Formula: see text] has small interactions and finite charge gaps, but, interestingly, it shows metallic nature at the same time. We obtain approximate phase diagrams for the entire parameter space and for band fillings equal to 1/2 and 1/3. Finally, we compare certain bosonization and field theory formulas with our quasi-exact numerical results, from which disagreements are found.

15.
J Nanosci Nanotechnol ; 19(6): 3669-3672, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30744804

RESUMO

Organic-inorganic hybrid perovskite single crystals have attracted much attention due to their superior optoelectronic properties. Herein, we report a facile vapor-solution sequential route to prepare single-crystalline nanosheets of hybrid lead triiodide perovskite. It is found that this two-step deposition is able to fabricate sizeable high-quality single-crystalline nanosheets with no need of delicate control of crystallization conditions such as concentration or temperature for normal single crystal growth. The resulting perovskite nanosheets show good reproducibility and single crystallinity with bright and uniform photoluminescence. Our study provides a promising strategy for scalable fabrication of perovskite single crystals with great potential in optoelectronic applications.

16.
Sci Total Environ ; 664: 45-52, 2019 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-30739852

RESUMO

Estimation of heterotrophic biomass concentration in activated sludge is essential to the design, operation and management of activated sludge process for wastewater treatment plants (WWTPs), and many methods have been developed for such a purpose. In this study, three respiration-based methods: the Exponential-growth-rate-based method (Exp-M), the Maximum-respiration-rate-based method (Max-M) and the Endogenous-respiration-rate-based method (End-M), which are frequently used for determining kinetic parameters in activated sludge models, were comparatively examined using experimental results from both full-scale municipal WWTPs and laboratory-scale reactors. Our study revealed the pros and cons of each method, which is valuable for method selection in different applications. The End-M can estimate all the fraction of biomass. However, the proper control of measuring condition is of great challenge. The Exp-M can only determine the exponential growth part of biomass as conditions employed during measuring may make a considerable part of biomass in a nongrowth status, resulting underestimation or even failure of calculation. The Max-M can determine the viable biomass including the nongrowth part, and it is recommended for rapid assessment of biomass. The Max-M was modified after the introduction of a coefficient SOURSRT=0 (the specific oxygen utilization rate when the sludge retention time was assumed zero) and was validated by using the experimental results reported in previous studies. Because of its simplicity and much improved accuracy, the modified Max-M method is able to provide more useful information about activated sludge compositions and has a promising application potential in wastewater treatment plants.


Assuntos
Eliminação de Resíduos Líquidos/métodos , Biomassa , Reatores Biológicos , Processos Heterotróficos , Cinética , Esgotos , Águas Residuárias
17.
World J Gastroenterol ; 25(1): 138-150, 2019 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-30643364

RESUMO

AIM: To evaluate the clinical properties of three subpopulations of circulating tumor cells (CTCs) undergoing epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PDAC) patients. METHODS: We identified CTCs for expression of the epithelial cell marker cytokeratin or epithelial cell adhesion molecule (EpCAM) (E-CTC), the mesenchymal cell markers vimentin and twist (M-CTC), or both (E/M-CTC) using the CanPatrol system. Between July 2014 and July 2016, 107 patients with PDAC were enrolled for CTC evaluation. CTC enumeration and classification were correlated with patient clinicopathological features and outcomes. RESULTS: CTCs were detected in 78.5% of PDAC patients. The number of total CTCs ranged from 0 to 26 across all 107 patients, with a median value of six. CTC status correlated with lymph node metastasis, TNM stage, distant metastasis, blood lymphocyte counts, and neutrophil-to-lymphocyte ratio (NLR). Kaplan-Meier survival analysis showed that patients with ≥ 6 total CTCs had significantly decreased overall survival and progression-free survival compared with patients with < 6 total CTCs. The presence of M-CTCs was positively correlated with TNM stage (P < 0.01) and distant metastasis (P < 0.01). Additionally, lymphocyte counts and NLR in patients without CTCs were significantly different from those in patients testing positive for each CTC subpopulation (P < 0.01). CONCLUSION: Classifying CTCs by EMT markers helps to identify the more aggressive CTC subpopulations and provides useful evidence for determining a suitable clinical approach.


Assuntos
Biomarcadores Tumorais/análise , Carcinoma Ductal Pancreático/patologia , Transição Epitelial-Mesenquimal , Células Neoplásicas Circulantes/patologia , Neoplasias Pancreáticas/patologia , Idoso , Carcinoma Ductal Pancreático/sangue , Carcinoma Ductal Pancreático/mortalidade , Molécula de Adesão da Célula Epitelial/análise , Feminino , Humanos , Estimativa de Kaplan-Meier , Biópsia Líquida/métodos , Masculino , Pessoa de Meia-Idade , Proteínas Nucleares/análise , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/mortalidade , Prognóstico , Proteína 1 Relacionada a Twist/análise , Vimentina/análise
18.
J Phys Condens Matter ; 31(12): 125402, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30650403

RESUMO

We propose a model of two-leg ladder topological insulator in which the spin-orbit couplings are presented in both intra-chain and inter-chain interactions. The topological phase supports four fractional charged edge states localized at opposite ends of the ladder, which belongs to the chiral symplectic (CII) class protected by time-reversal symmetry and chiral symmetry. In our model, the presence of time-reversal and chiral symmetry generates fourfold degeneracy for the edge states, and the two edge states with same chirality at one end of the ladder each carries half charge. In contrast to the two edge states spatially localized at one end of the ladder being not distinguished, these two edge states can be detected by the momentum density. The experimental scheme for realizing our model with cold atoms in optical lattice is discussed. By introducing a magnetic field in the x direction, the system is driven from CII class to AIII class. In AIII class, there exist two distinct topological phases that exhibit four degenerate edge states and two degenerate edge states in the gap, respectively. As same as the system in CII class, each edge state carries a half charge in AIII class.

19.
Environ Technol ; 40(27): 3622-3631, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29855222

RESUMO

Granular size plays a key role in the performance of the aerobic granular sludge (AGS). As the diameter of the granule increases, stratification may begin to appear due to the increase in mass transfer resistance. Aerobic granules harvested from a lab-scale anaerobic-aerobic sequencing batch reactor (AO-SBR) were classified into three categories according to their size: (a) 0.15-0.28 mm, (b) 0.28-0.45 mm and (c) larger than 0.45 mm. In this study, the categories were called small-size, medium-size and large-size granules, respectively. A fraction of the different forms of phosphate and denitrification efficiency was investigated in each category. Results show that small-size granules present much more easily mobile phosphorus than other granules. Moreover, the denitrification performance has been tested by using dumping and trickling patterns for COD and NO3--N feeding. The results demonstrated that the large-size granules exhibit poor denitrification rates, as opposed to the medium-size granules. Therefore, medium-size granules, with a size of 0.28-0.45 mm, are regarded as the most suitable granular size for AGS in this experiment from the perspective of denitrification and phosphorus removal.


Assuntos
Fósforo , Esgotos , Aerobiose , Reatores Biológicos , Desnitrificação , Nitrogênio , Eliminação de Resíduos Líquidos
20.
J Neurosci Rural Pract ; 9(2): 268-271, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29725182

RESUMO

Cerebral cysticercosis is common, but the possibility for repeated occurrence of peritoneal catheter blockage caused by neurocysticercosis (NCC) after two revisions following ventriculoperitoneal shunt placement for hydrocephalus is unusual. Herein, we describe one rare case in which peritoneal catheter revision was performed two times unsuccessfully. Endoscopic cysternostomy rather than peritoneal catheter adjustment was performed successfully, and histopathological examination of excised cystic samples confirmed NCC in our hospital. The present case highlights the need for awareness of NCC as a possible etiology of hydrocephalus, especially in developing countries. Uncommon findings in both lateral ventricles following low-field magnetic resonance imaging scans as well as the rarity of this infection involved in unusual location play important roles in misdiagnosis and incorrect treatment for hydrocephalus; thus, endoscopic cysternostomy, rather than multiple shunt adjustment of the peritoneal end, is recommended in the selected patient. To the best of our knowledge, this is the first report describing the misdiagnosis and inappropriate treatment of hydrocephalus caused by cerebral cysticercosis in China.

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