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1.
Clin Otolaryngol ; 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32058675

RESUMO

OBJECTIVES: To evaluate the clinical efficacy of a high-efficiency air purifier in patients with allergic rhinitis. DESIGN: We conducted a randomized, double-blind, clinical controlled trial with active and inactive versions of an air purifier. Our study included patients with allergic rhinitis who were sensitive to Artemisia pollen and treatment of the indoor environment using air filtration at night. We evaluated the clinical efficacy of indoor air filtration during the Artemisia pollen scattering season in Yulin City in Shangxi Province, China. SETTING: The First Hospital of Yulin. (Yulin City, Shanxi Province, China). PARTICIPANTS: A total of 90 patients with allergic rhinitis who were sensitive to allergens of Artemisia pollen were randomly assigned to one of two groups in equal numbers. MAIN OUTCOME MEASURES: The primary outcome measure was the difference in visual analog scale scores from baseline. Secondary outcomes were changes from baseline in nasal symptoms, allergy symptom scores, responses to the Rhinoconjunctivitis Quality of Life Questionnaire, Epworth Sleepiness Scale scores, and tolerability scores for the air purifier. RESULTS: Based on the allergy symptom score, we found significant differences in rhinitis symptoms between the groups who used the active versus the inactive air purifier. CONCLUSIONS: The results of our investigation demonstrated the health benefits of particle filtration.

2.
Eur J Pharmacol ; 874: 172990, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32057718

RESUMO

Ovarian carcinoma (OC) begins in the ovaries and remains a highly lethal malignancy. Despite great efforts have been made to fight against OC, there still remain limited therapeutic options owing to chemotherapy drug resistance and serious side effects. Osthole is a derivative of coumarin and extracted from Cnidium monnieri (L.) Cusson, which has been drawn more attention due to its high biological activity in various disease. However, the underlying mechanism of osthole in OC is still unclear. In this study, we aim to evaluate the mechanism of osthole against OC cells. Methodologically, Cell Counting Kit-8 (CCK-8) and LIVE/DEAD™ Cell Imaging experiments were employed to assess cell viability. 2',7'-Dichlorofluorescin diacetate (DCFH-DA) staining, flow cytometry, Hoechst staining, JC-1 staining assay and western blotting were performed to study apoptosis. Transmission electron microscopy, western blotting and monodansyl cadaverine (MDC) staining assay were used to study autophagy. Western blotting and microscopy image were employed to determine pyroptosis. Our results demonstrated that osthole could significantly suppress OC cells growth in a dose-dependent manner. We further proved that osthole could inhibit OC cells growth by mitochondria-mediated apoptosis. Meanwhile, we also discovered that osthole could trigger cell autophagy and lead to cell death. Furthermore, our study revealed that osthole could lead to pyroptosis through inducing the cleavage of gasdermin E (c-GSDME) level. Taken together, Osthole could significantly suppress the growth of OC cells and induce OC cells death via apoptosis, pyroptosis and autophagy, which is a promising new drug for the treatment of OC.

3.
Oncogene ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32015484

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

4.
Addict Biol ; : e12875, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031744

RESUMO

The development of opioid addiction involves DNA methylation. Accordingly, the DNA demethylation, induced by ten-eleven translocation (Tet) enzymes, may represent a novel approach to prevent opioid addiction. The present study examined the role of TET1 and TET3 in the development of morphine-seeking behavior in rats. We showed that 1 day of morphine self-administration (SA) training upregulated TET3 but not TET1 expression in the hippocampal CA1. With 7 days of morphine SA training, the expression of TET3 in the CA1 returned to the baseline level, while the TET1 expression was downregulated. No change of TET1 and TET3 in the nucleus accumbens shell was observed in morphine SA trained rats, or in the yoked morphine rats, or in rats trained for saccharin SA. Furthermore, we found that knocking down TET3 expression in the CA1 accelerated the acquisition of morphine SA, while overexpression of the catalytic domain of TET1 in the CA1 attenuated the acquisition. Together, these findings suggest that TET1 and TET3 in the CA1 are important epigenetic modulators involved in the morphine-seeking behavior and provide a new strategy in the treatment of opioid addiction.

5.
BMC Geriatr ; 20(1): 41, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32013915

RESUMO

BACKGROUND: With the fast-paced aging and increasing digitalization of society, there has been a growing interest in the effect of mobile device use on cognitive function and depression in older adults. However, research examining this issue among older adults in residential care homes (RCHs) is scant. Therefore, this study aimed to examine the impact of mobile device use on the cognitive function and depressive symptoms of older adults living in RCHs. METHODS: A cross-sectional survey was conducted using a sociodemographic questionnaire, the Montreal Cognitive Assessment (MoCA) and the 15-item Geriatric Depression Scale (GDS-15). RESULTS: A total of 235 senior residents (aged 82.58 ± 5.54) in four RCHs were surveyed. Users of mobile devices had a significantly higher total MoCA score (25.02 ± 4.14) and a significantly lower GDS-15 score (3.28 ± 2.74) than non-users (MoCA: 19.34 ± 5.21, GDS-15: 4.69 ± 2.90). Multivariate linear regression indicate that mobile device use is significantly associated with total MoCA score, six of the seven sub-scores (visuospatial abilities and execution functions, attention, language, abstraction, delayed recall, and orientation)(P < 0.05). Logistic regression showed that mobile device use was significantly associated with the level of depressive symptoms (OR = 0.458, 95%CI = 0.249-0.845). CONCLUSIONS: Use of mobile devices has a significant association with the cognitive function and depressive symptoms of older adults living in RCHs, and thus should be encouraged as a measure to maintain and improve cognition and prevent depression.

6.
Chin Med J (Engl) ; 133(3): 292-300, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31904729

RESUMO

BACKGROUND: Baicalein has been shown to have anti-inflammatory and anti-tumor activities. However, the mechanisms underlying its anti-inflammatory effect on colitis remain unclear. METHODS: A dextran sodium sulfate (DSS)-induced model of acute colitis was established in BALB/c mice (6-8 weeks old, weighing 18-22 g). Six groups of mice received: (1) water for 10 days (control), n = 6; (2) DSS 4% solution in the drinking water for 7 days, followed by normal water for 3 days, n = 7; (3), (4), and (5) as for group 2 plus baicalein (10, 20, 40 mg/kg) administered once daily starting on day 1, n = 6; and (6) as for (2) plus 5-aminosalicylic acid (50 mg/kg) administered once daily starting on day 1, n = 6. Body weights, stool consistency, and hematochezia were recorded, and the severity of colitis was evaluated using a disease activity index. On day 11, the mice were euthanized, and organs and blood were collected for analysis. Serum inflammatory factors were detected by enzyme-linked immunosorbent assay; CD11b-positive cells were analyzed by immunofluorescence microscopy; expression of retinoic-acid-receptor-related orphan nuclear receptor gamma, sphingosine kinase 1 (SPHK1), and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) was detected by immunohistochemistry; and expression of nucleotide-binding oligomerization domain 2 (NOD2), SPHK1, sphingosine 1-phosphate receptor 1 (S1PR1), total STAT3, and p-STAT3 were detected by western blotting analysis. Inter-group differences were compared using Student's t test. RESULTS: Baicalein treatment dose-dependently reduced DSS-induced weight loss (P < 0.01 or P < 0.05), splenomegaly (P < 0.01), and colonic damage, as reflected by amelioration of diarrhea, rectal bleeding, and colonic ulceration, congestion, edema (shown as colon length, P < 0.05 or P < 0.01), and inflammatory cell infiltration. Baicalein also significantly decreased the levels of inflammatory mediators in the serum (P < 0.01) and colon, and significantly inhibited expression of NOD2 SPHK1, S1PR1, and p-STAT3 in the colon (P < 0.05). CONCLUSIONS: Baicalein treatment ameliorated colitis in mice by inhibiting S1P-STAT3 signaling, suggesting that this flavonoid might be beneficial in the treatment of colitis.

7.
J Environ Manage ; 261: 110157, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31999611

RESUMO

H2S-based denitrification could achieve synchronous removal of nitrate and H2S and had been regarded as an efficient way for biogas desulfurization and wastewater denitrification. Using CO2 in biogas as carbon source had a potential of saving cost further, but the performance deteriorated due to the drop in pH. Two kinds of nature ore, medical stone and phosphate ore, were added as new pH adjustment materials in this study, and feasibility of using CO2 as sole carbon source for H2S-based denitrification was investigated. As a result, both materials could increase the pH from 4.5 to above 6.0. Compared with medical stone, higher level of pH (up to 6.39) and nitrate removal efficiency (99.1%) were obtained with phosphate ore. In addition, ATP increased more rapidly than the control, reflecting improvement on microbial activities. Therefore, phosphate ore as the pH adjustment material could improve H2S-based denitrification performance obviously.

8.
J Clin Lab Anal ; : e23192, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31981248

RESUMO

BACKGROUND: The correlation between preoperative lipid profiles and new-onset diabetes after transplantation (NODAT) remains relatively unexplored in liver transplant recipients (LTRs). Thus, we aimed to investigate the preoperative lipid profiles in Chinese LTRs and evaluate the different influences of preoperative total cholesterol, total triglycerides (TG), high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol on the development of NODAT in both sexes. METHODS: A total of 767 Chinese LTRs from Zhongshan Hospital were retrospectively evaluated. NODAT was defined according to the American Diabetes Association guidelines; the relationship between each preoperative lipid index and NODAT development was analyzed separately in men and women. RESULTS: Pretransplant hypotriglyceridemia was observed in 35.72% of the total LTRs. In men, only the preoperative TG level was significantly associated with incident NODAT after adjusting for potential confounders (hazard ratio 1.37, 95% confidence interval 1.13-1.66, P = .001). There was a nonlinear relationship between the preoperative TG level and NODAT risk. The risk of NODAT significantly increased with preoperative a TG level above 0.54 mmol/L (log-likelihood ratio test, P = .043). In women, no significant association was observed. CONCLUSION: Among male LTRs, a higher preoperative TG level, even at a low level within the normal range, was significantly and nonlinearly associated with an increased risk of NODAT.

9.
Plant Foods Hum Nutr ; 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31925636

RESUMO

Two selected walnut cultivars (Xiangling and Jizhaomian) growing in China were analyzed in terms of proximate and mineral composition, fatty acid and amino acid profile. According to the higher kernel rate (61.22%), thinner shell (1.03 mm), higher content of fat (66.93%), protein (20.97%), essential amino acids (29.31% of total amino acids) and minerals such as manganese, zinc and copper, Xiangling would be the more nutritive cultivar with more commercial value. However, Jizhaomian would be the more healthful cultivar based on the higher content of polyunsaturated fatty acids (79.39% of total fatty acids), and it would be more sensitive to rancidity. Jizhaomian also showed higher content in potassium, calcium, magnesium and iron. Genotype is the major source of variability in morphology and chemical compositions. Regardless of the cultivar, consumption of walnuts is desirably encouraged, since all of them contain compounds potentially beneficial to health.

10.
Anal Bioanal Chem ; 412(4): 905-913, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31897560

RESUMO

The ensemble system PyH-SBA-15-Cu2+ was obtained via coordination interaction of pyrene derivative-functionalized mesoporous SBA-15 and Cu2+, and applied for the selective and sensitive detection of H2S over pH 6.0-12.0 in aqueous media. The sensing strategy was designed on the basis of the H2S-induced dissolution of Cu2+ from PyH-SBA-15-Cu2+. Cu2+ has good binding affinity to N atoms in PyH-SBA-15; therefore, the organic-inorganic hybrid ensemble PyH-SBA-15-Cu2+ was formed, which is nonfluorescent in aqueous solution because of the Cu2+-promoted emission quenching of PyH-SBA-15. The addition of H2S induces the dissolution of PyH-SBA-15-Cu2+ by the formation of stable CuS, thereby producing fluorescence revival of PyH-SBA-15. The correlative "turn-on" fluorescence signals of this ensemble system are linearly proportional to [H2S] in the concentration region of 0-1.0 × 10-4 M, showing a low detection limit of 3.7 × 10-7 M. Other common anions do not induce distinct fluorescence changes. When using the fluorescence intensity signal changes of PyH-SBA-15 as outputs and Cu2+ and S2- as inputs, PyH-SBA-15 can act as an XNOR logic gate.

11.
J Enzyme Inhib Med Chem ; 35(1): 468-477, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31902266

RESUMO

C-Met plays a crucial role in the development and progression of neoplastic disease. Type II c-Met inhibitors recognise the inactive DFG-out conformation of the kinase, result in better anti-tumour effects due to synergistic effect against the other kinases. According to our previous works, an (E)-N'-benzylidene group was selected as the initial fragment. Two series of (E)-N'-benzylidene hydrazides were designed by fragment growth method. The inhibitory activities were in vitro investigated against c-Met and VEGFR-2. Compound 10b exhibited the most potent inhibitory activity against the c-Met inhibitor (IC50 = 0.37 nM). Compound 11b exhibited multi-target c-Met kinase inhibitory activity as a potential type II c-Met inhibitor (IC50 = 3.41 nM against c-Met; 25.34 nM against VEGFR-2). The two compounds also demonstrate the feasibility of fragment-based virtual screening method for drug discovery.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31990992

RESUMO

MicroRNAs (miRNAs) have emerged as critical regulators of neuronal survival during cerebral ischaemia/reperfusion injury. Accumulating evidence has shown that miR-199a-5p plays a crucial role in regulating apoptosis and survival in various cell types. However, whether miR-199a is involved in regulating neuronal survival during cerebral ischaemia/reperfusion injury remains unknown. In this study, we aimed to explore the biological role of miR-199a-5p in regulating neuronal injury induced by oxygen-glucose deprivation/reoxygenation (OGD/R), an in vitro cellular model of cerebral ischaemia and reperfusion injury. We found that miR-199a-5p expression was significantly altered in neurons in response to OGD/R treatment. Overexpression of miR-199a-5p facilitated OGD/R-induced apoptosis and reactive oxygen species (ROS) production, whereas miR-199a-5p inhibition alleviated OGD/R-induced apoptosis and ROS production. Notably, our results identified Brahma-related gene 1 (Brg1) as a target gene of miR-199a-5p. Moreover, inhibition of miR-199a-5p promoted the activation of nuclear factor-erythroid-2-related factor-2 (Nrf2)/heme oxygenase-1 (HO-1) signalling via targeting Brg1. However, silencing of Brg1 markedly reversed the miR-199a-5p inhibition-mediated neuroprotective effect. Taken together, our results suggest that downregulation of miR-199a-5p protects neurons from OGD/R-induced neuronal injury through upregulating Brg1 to activate Nrf2/HO-1 signalling. The miR-199a-5p/Brg1/Nrf2/HO-1 regulation axis may play an important role in regulating neuronal survival during cerebral ischaemic/reperfusion injury in vivo.

13.
Phytochemistry ; 170: 112212, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31785552

RESUMO

Phenylpropanoids comprise a broad spectrum of biologically active natural products. As part of our ongoing research on antiepileptic active compounds from traditional Chinese herb, Acorus calamus var. angustatus Besser, three undescribed phenylpropanoids and twenty-two known ones were isolated. All the undescribed structures were determined by a combination of 1D and 2D NMR, HRMS. In addition, γ-asaronol was identified as racemates and its absolute configuration were determined by the modified Mosher's method and ECD spectral data. Furthermore, some selected isolated compounds were evaluated for their cell viability and neuroprotective activities in H2O2-induced SH-SY5Y cells. α-Asaronol, ß-asaronol, 3-(2,4,5-trimethoxyphenyl)propan-1-ol and 1,2,4-trimethoxy-5-(3-methoxypropyl)benzene exerted potential protective activity from neuronal oxidative stress in all test concentrations ranging from 0.01 to 100 µM, in which the neuroprotective activity of ß-asaronol was the best.

14.
J Cell Biochem ; 121(2): 930-937, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31588631

RESUMO

Histone deacetylase 4 (HDAC4) is a member of the HDACs family, its expression is closely related to the cell development. The cell is an independent living entity that undergoes proliferation, differentiation, senescence, apoptosis, and pathology, and each process has a strict and complex regulatory system. With deepening of its research, the expression of HDAC4 is critical in the life process. This review focuses on the posttranslational modification of HDAC4 in cell biology, providing an important target for future disease treatment.

15.
J Hazard Mater ; 384: 121239, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31574384

RESUMO

Incorporating bioaccessibility into human health risk assessment is recognized as a valid way to reduce the conservative properties of conventional results, where the total concentration of a contaminant analysed by exhaustive chemical extraction is applied. Taking a coke production site in Beijing as an example, a mild chemical extraction technology was employed to profile the bioaccessibility of benzo[a]pyrene (BaP), indeno[1,2,3-cd]pyrene (IcP) and dibenz[ah]anthracene (DBA) in soils. The results that were regressed using two bi-phase desorption models (Karickhoff and Weibull) revealed that the rapid desorption fractions of BaP, IcP and DBA, which are taken for bioaccessible fractions, were basically less than half of the total contents in the soils. Probabilistic analysis (PA) was carried out with pre-set distributions of the exposure parameters to characterize the uncertainty in the assessment. The results incorporating bioaccessibility and PA were several times higher than the generic remediation goals which equal to national screening levels, and orders of magnitude higher than the baselines of the region and nation. The results of the Weibull fit were finally recommended as site-specific remediation goals (SSRGs) (10.59 mg/kg, 95.48 mg/kg and 9.24 mg/kg). Over-remediation was avoided while contributing to considerable economic and environmental benefits.

16.
Bioorg Chem ; 94: 103385, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31669094

RESUMO

A series of homoerythrina alkaloid derivatives containing a 1,2,3-triazole moiety as PARP-1 inhibitors were designed and synthesized. And their anti-proliferative activity was further evaluated. Compound 10n had excellent activity to inhibit proliferation of A549 cells (IC50 = 1.89 µM), which was higher than harringtonine (IC50 = 10.55 µM), pemetrexed (IC50 = 3.39 µM), and rucaparib (IC50 = 4.91 µM). Furthermore, the selectivity index of compound 10n was higher than rucaparib and pemetrexed for lung cancer cells. Flow cytometry analysis showed that compound 10n significantly arrested the cell cycle in the S phase, then induced apoptosis of A549 cells (apoptosis rate is 46%), which effectively inhibited cell proliferation. Simultaneously, western blot analysis revealed that compound 10n could prevent the biosynthesis of PAR. Further analysis results revealed that compound 10n could inhibit the expression of cyclin A, down-regulate the expression of bcl-2/bax, activate caspase-3, and ultimately induce apoptosis of A549 cells. All the results indicated that compound 10n had potential research value as a novel PARP-1 inhibitor in antitumor, and it provided a new reference for further development of PARP-1 inhibitors.

17.
J Enzyme Inhib Med Chem ; 35(1): 235-244, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31760818

RESUMO

Cyclin-dependent kinase 2 (CDK2) is the family of Ser/Thr protein kinases that has emerged as a highly selective with low toxic cancer therapy target. A multistage virtual screening method combined by SVM, protein-ligand interaction fingerprints (PLIF) pharmacophore and docking was utilised for screening the CDK2 inhibitors. The evaluation of the validation set indicated that this method can be used to screen large chemical databases because it has a high hit-rate and enrichment factor (80.1% and 332.83 respectively). Six compounds were screened out from NCI, Enamine and Pubchem database. After molecular dynamics and binding free energy calculation, two compounds had great potential as novel CDK2 inhibitors and they also showed selective inhibition against CDK2 in the kinase activity assay.


Assuntos
Antineoplásicos/análise , Antineoplásicos/farmacologia , Quinase 2 Dependente de Ciclina/antagonistas & inibidores , Simulação de Acoplamento Molecular , Inibidores de Proteínas Quinases/análise , Inibidores de Proteínas Quinases/farmacologia , Máquina de Vetores de Suporte , Células A549 , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Quinase 2 Dependente de Ciclina/metabolismo , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Ensaios de Seleção de Medicamentos Antitumorais , Células HCT116 , Humanos , Estrutura Molecular , Inibidores de Proteínas Quinases/química , Relação Estrutura-Atividade
18.
Proc Natl Acad Sci U S A ; 117(1): 221-227, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31857387

RESUMO

The recent discovery of the interfacial superconductivity (SC) of the Bi2Te3/Fe1+yTe heterostructure has attracted extensive studies due to its potential as a novel platform for trapping and controlling Majorana fermions. Here we present studies of another topological insulator (TI)/Fe1+yTe heterostructure, Sb2Te3/Fe1+yTe, which also has an interfacial 2-dimensional SC. The results of transport measurements support that reduction of the excess Fe concentration of the Fe1+yTe layer not only increases the fluctuation of its antiferromagnetic (AFM) order but also enhances the quality of the SC of this heterostructure system. On the other hand, the interfacial SC of this heterostructure was found to have a wider-ranging TI-layer thickness dependence than that of the Bi2Te3/Fe1+yTe heterostructure, which is believed to be attributed to the much higher bulk conductivity of Sb2Te3 that enhances indirect coupling between its top and bottom topological surface states (TSSs). Our results provide evidence of the interplay among the AFM order, itinerant carries from the TSSs, and the induced interfacial SC of the TI/Fe1+yTe heterostructure system.

19.
Gait Posture ; 76: 157-161, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31862663

RESUMO

BACKGROUND: Center of pressure (COP) trajectory during treadmill walking have been commonly presented using the butterfly diagram to describe gait characteristics in neurologically intact and impaired individuals. However, due to the large amount of displayed information, the butterfly diagram is not an efficient solution to visualize locomotor variability. PURPOSE: The purpose of this study was to evaluate post-stroke locomotor variability by applying Kernel density estimation (KDE) on the intersections of the butterfly diagram, and to compare KDE derived metrics with conventional metrics of gait symmetry and variability. METHODS: Bilateral toe-off (TO) and initial contact (IC) points of the butterfly diagram were determined to calculate the COP symmetry index and the intersections of bilateral TOIC. Subsequently, the intersections during the walking window were used to evaluate its density and variability by Kernel density estimation. Standard deviations of step width and step length were compared between groups. RESULTS: Using the KDE surface plots we observed 4 characteristically different patterns with individuals post-stroke, which were associated with functional status quantified using walking speed and lower extremity Fugl-Meyer scores. However, locomotor variability quantified using standard deviations of step width and lengths did not differ between groups. Significance & Novelty: This paper presents a novel approach of using KDE analysis as a better and more sensitive method to characterize locomotor COP variability in individuals with post-stroke hemiparesis, compared to conventional metrics of gait symmetry and variability.

20.
Cell Transplant ; : 963689719890597, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31822119

RESUMO

Ovarian cancer is the leading cause of death among gynecological malignancies. Cyclooxygenase 2 is widely expressed in various cancer cells and participates in the occurrence and development of tumors by regulating a variety of downstream signaling pathways. However, the function and molecular mechanisms of cyclooxygenase 2 remain unclear in ovarian cancer. Here, we demonstrated that cyclooxygenase 2 was highly expressed in ovarian cancer and the expression level was highly correlated with ovarian tumor grades. Further, ovarian cancer cells with high expression of cyclooxygenase 2 exhibit enhanced proliferation and invasion abilities. Specifically, cyclooxygenase 2 promoted the release of prostaglandin E2 upregulated the phosphorylation levels of phospho-nuclear factor-kappa B p65. Celecoxib, AH6809, and BAY11-7082 all can inhibit the promoting effect of cyclooxygenase 2 on SKOV3 and OVCAR3 cell proliferation and invasion. Besides, celecoxib inhibited SKOV3 cell growth in the xenograft tumor model. These data suggest that high expression of cyclooxygenase 2 promotes the proliferation and invasion of ovarian cancer cells through the prostaglandin E2/nuclear factor-kappa B signaling pathway. Cyclooxygenase 2 may be a potential therapeutic target for the treatment of ovarian cancer.

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