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1.
Eur J Med Res ; 28(1): 60, 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36732805

RESUMO

BACKGROUND: Referring to the International Society of Nephrology/Renal Pathology Society (ISN/RPS) 2018 pathological classification, we aim to reveal the significance of cellular/fibrocellular crescents in lupus nephritis (LN) patients. METHODS: Patients with LN proven by renal biopsy at the First Affiliated Hospital of Wenzhou Medical University from December 2001 to November 2017 were identified, and eligible cases were divided into two groups according to the presence or absence of cellular/fibrocellular crescents in renal biopsy tissues. RESULTS: A total of 401 LN patients were identified from our follow-up database, and 296 eligible LN patients were enrolled in the study. Of these patients, 146 patients in the group without cellular/fibrocellular crescents (non-crescent group) and 150 patients in the group with cellular/fibrocellular crescents (Crescent group). The median follow-up time of patients was 47 months, and a total of 54 patients progressed to the composite endpoint. Crescent group had higher serum creatinine, lower serum albumin, higher systemic lupus erythematosus (SLE) disease activity index, and higher activity index of renal tissue. The interaction between cellular/fibrocellular crescents and proteinuria at baseline was associated with the prognostic risk of LN (P = 0.006). In the group with proteinuria < 3.5 g/24 h, the prognosis of crescent group was significantly worse than of non-crescent group (P < 0.001), while in the group with proteinuria ≥ 3.5 g/24 h, there was no significant relationship between crescents and prognosis (p = 0.452). By multivariable Cox hazard analysis, positive anti-dsDNA, chronic index of renal biopsy tissue, cellular/fibrocellular crescents and its interaction with 24 h proteinuria were independent risk factors for poor prognosis of LN. CONCLUSIONS: LN patients with cellular/fibrocellular crescents had more severe and active disease features, and cellular/fibrocellular crescents is a risk factor for poor prognosis of LN. There was an interaction between cellular/fibrocellular crescents and proteinuria in predicting poor prognosis, and among patients with low levels of proteinuria at the time of renal biopsy, those with crescents had a worse long-term prognosis than those without crescents.

2.
Nature ; 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36697830

RESUMO

Indirect development with an intermediate larva exists in all major animal lineages1, which makes larvae central to most scenarios of animal evolution2-11. Yet how larvae evolved remains disputed. Here we show that temporal shifts (that is, heterochronies) in trunk formation underpin the diversification of larvae and bilaterian life cycles. We performed chromosome-scale genome sequencing in the annelid Owenia fusiformis with transcriptomic and epigenomic profiling during the life cycles of this and two other annelids. We found that trunk development is deferred to pre-metamorphic stages in the feeding larva of O. fusiformis but starts after gastrulation in the non-feeding larva with gradual metamorphosis of Capitella teleta and the direct developing embryo of Dimorphilus gyrociliatus. Accordingly, the embryos of O. fusiformis develop first into an enlarged anterior domain that forms larval tissues and the adult head12. Notably, this also occurs in the so-called 'head larvae' of other bilaterians13-17, with which the O. fusiformis larva shows extensive transcriptomic similarities. Together, our findings suggest that the temporal decoupling of head and trunk formation, as maximally observed in head larvae, facilitated larval evolution in Bilateria. This diverges from prevailing scenarios that propose either co-option9,10 or innovation11 of gene regulatory programmes to explain larva and adult origins.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36646313

RESUMO

STUDY OBJECTIVE: To describe hysteroscopy-assisted transvaginal repair technique without scar defect resection for uterine niche. DESIGN: Surgical video article. Stepwise narrated video demonstration of the novel technique. A total of 15 women diagnosed with niche in the uterus were enrolled in our study. Patients provided signed consent and there are no conflicts of interest. SETTING: Niche in the uterus is defined as an indentation in the myometrium at the uterine incision due to healing defects [1]. Surgical treatment options for niche include hysteroscopic, laparoscopic and vaginal surgery [2]. Compared with hysteroscopic surgery, regular vaginal surgery may effectively increase the residual myometrium thickness (RMT), reducing the risk of subsequent pregnancy [3-5]. However, regular vaginal surgery removes the original scar defect followed by suture, which can lead to a new niche after the surgery and may postpone conception because of the new uterine incision [6]. Here we describe a new technique of hysteroscopy-assisted transvaginal repair for the niche, which does not remove the original scar defect [7]. This surgery may be beneficial for those who plan conceive as soon as possible after the operation. INTERVENTIONS: Hysteroscopy-assisted vaginal surgery without scar defect resection was selected as ideal surgical approach. Hysteroscopy was used for abnormal structures in the niche [8,9]. Opening the peritoneum through the vaginal wall confirmed the position of the niche and a 2-0 absorbable suture was used to interruptedly suture the upper and lower margins of the scar defect and close the niche (or reduce its size), rather than resection. Hysteroscopy was used again to assess the status of the niche after suture and confirm repair. If unsatisfactory, suture procedure was repeated to close the remaining part of the niche. CONCLUSION: Our novel technique, described and demonstrated in this video article, is an efficacious and viable approach to treat uterine niche. Chinese experts recommend at least one year gap between conventional scar defect resection with suturing and conception, because of the new uterine incision [6]. In contrast, this novel procedure avoids removal of the original scar defect and the surrounding scar tissue by directly repairing the lower uterine segment. Meanwhile, hysteroscopy can treat abnormal structures in the niche and improve effectiveness. Since the integrity of the uterine myometrium is retained, this technique may help shorten contraception time after operation. Further studies with larger sample size, longer follow-up time and more postoperative follow-up indicators, such as fertility outcomes, are needed to comprehensively evaluate the restorative effect of this novel technique.

4.
Entropy (Basel) ; 25(1)2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36673274

RESUMO

This study considers the change point testing problem in autoregressive moving average (ARMA) (p,q) models through the location and scale-based cumulative sum (LSCUSUM) method combined with neural network regression (NNR). We estimated the model parameters via the NNR method based on the training sample, where a long AR model was fitted to obtain the residuals. Then, we selected the optimal model orders p and q of the ARMA models using the Akaike information criterion based on a validation set. Finally, we used the forecasting errors obtained from the selected model to construct the LSCUSUM test. Extensive simulations and their application to three real datasets show that the proposed NNR-based LSCUSUM test performs well.

5.
Thorac Cancer ; 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36597175

RESUMO

BACKGROUND: MicroRNAs (miRNAs) play crucial roles in the development of various cancers. Here, we aimed to evaluate the roles of miR-138-5p in lung cancer progression and the value of miR-138-5p in lung cancer diagnosis. METHODS: Quantitative real-time PCR was performed to examine the expressions of miR-138-5p and smad nuclear interacting protein 1 (SNIP1) mRNA. The diagnostic value of miR-138-5p was analyzed using receiver operating characteristic (ROC) curve analysis, sensitivity, and specificity. We explored the effect of miR-138-5p on cell proliferation and metastasis by CCK-8, colony formation, wound healing and transwell assays. Western blot was employed to detect the protein expression of SNIP1 and related genes. Lung cancer cell growth was evaluated in vivo using xenograft tumor assay. RESULTS: MiR-138-5p was decreased in the serum of patients with non-small cell lung cancer (NSCLC) and in NSCLC cells and tissues. The area under the ROC curve of serum miR-138-5p in the diagnosis of NSCLC was 0.922. This finding indicates the high diagnostic efficiency for lung cancer. MiR-138-5p suppressed but its inhibitor promoted cell proliferation and migration compared with control treatment in vitro and in vivo. MiR-138-5p directly binds to the 3'-untranslated region of SNIP1 and negatively regulated the expression of SNIP1, thereby inhibiting the expression of cyclin D1 and c-Myc. Moreover, overexpression of SNIP1 rescues the miR-138-5p-mediated inhibition in NSCLC cells. CONCLUSIONS: The results suggested that miR-138-5p suppressed lung cancer cell proliferation and migration by targeting SNIP1. Serum miR-138-5p is a novel and valuable biomarker for NSCLC diagnosis.

6.
J Environ Manage ; 328: 116943, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36516715

RESUMO

Biochar is widely used as a soil amendment due to its environmental friendliness and convenient availability. It is believed that the presence of biochar in porous media can influence the transport of colloidal and solute contaminants. In this study, different mass ratios of biochar were added to packed sand with a rough or smooth surface to determine the significance of biochar on the retention and release of silver nanoparticles (AgNPs). The results showed that biochar reduced the transport of AgNPs in rough and smooth sands under different solution conditions. A small amount of biochar (0.1-1% in mass percentage) can significantly enhance the retention of AgNPs due to the alteration in collector surface roughness and chemical heterogeneity that potentially reduce the energy barrier for retention. Furthermore, the retention of AgNPs in rough sand was always higher than that in smooth sand under the same experimental conditions. The presence of biochar also produced nonmonotonic retention of AgNPs mainly due to the changes in collector surface roughness. Additionally, the AgNPs retention associated with biochar tended to be irreversible due to the charge heterogeneity, while the reversible retention could mainly occur on a rough sand surface via shallow primary minima. This work highlights the significance of collector surface roughness that needs to be considered in the process of biochar amendment for practical applications to effectively immobilize colloidal contaminants in soil or groundwater.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Areia , Nanopartículas Metálicas/química , Prata/análise , Porosidade , Propriedades de Superfície , Solo
7.
Nat Genet ; 55(1): 144-153, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36581701

RESUMO

Networks are powerful tools to uncover functional roles of genes in phenotypic variation at a system-wide scale. Here, we constructed a maize network map that contains the genomic, transcriptomic, translatomic and proteomic networks across maize development. This map comprises over 2.8 million edges in more than 1,400 functional subnetworks, demonstrating an extensive network divergence of duplicated genes. We applied this map to identify factors regulating flowering time and identified 2,651 genes enriched in eight subnetworks. We validated the functions of 20 genes, including 18 with previously unknown connections to flowering time in maize. Furthermore, we uncovered a flowering pathway involving histone modification. The multi-omics integrative network map illustrates the principles of how molecular networks connect different types of genes and potential pathways to map a genome-wide functional landscape in maize, which should be applicable in a wide range of species.


Assuntos
Proteômica , Zea mays , Zea mays/genética , Genômica , Genes de Plantas
8.
Int J Pharm ; 631: 122488, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36521638

RESUMO

Reduced drug uptake and elevated drug efflux are two major mechanisms in cancer multidrug resistance (MDR). In the present study, a new multistage O2-producing liposome with NAG/R8-dual-ligand and stimuli-responsive dePEGylation was developed to address the abovementioned issues simultaneously. The designed C-NAG-R8-PTXL/MnO2-lip could also achieve magnetic resonance imaging (MRI)-guided synergistic chemodynamic/chemotherapy (CDT/CT). In vitro and in vivo studies showed that C-NAG-R8-PTXL/MnO2-lip enhanced circulation time by PEG and targeted the tumor site. After tumor accumulation, endogenous l-cysteine was administered, and the PEG-attached disulfide bond was broken, resulting in the dissociation of PEG shells. The previously hidden positively charged R8 by different lengths of PEG chains was exposed and mediated efficient internalization. In addition, the oxygen (O2) generated by C-NAG-R8-PTXL/MnO2-lip relieved the hypoxic environment within the tumor, thus reducing the efflux of chemotherapeutic drug. O2 was able to burst liposomes and triggered the release of PTXL. The toxic hydroxyl radical (·OH), which was produced by H2O2 and Mn2+, strengthened CDT/CT. C-NAG-R8-PTXL/MnO2-lip was also used as MRI contrast agent, which blazed the trail to rationally design theranostic agents for tumor imaging.


Assuntos
Lipossomos , Neoplasias , Humanos , Lipossomos/química , Compostos de Manganês/química , Linhagem Celular Tumoral , Peróxido de Hidrogênio , Óxidos/química , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Resistência a Múltiplos Medicamentos , Oxigênio , Imageamento por Ressonância Magnética , Microambiente Tumoral , Nanomedicina Teranóstica
9.
Cancer Cell Int ; 22(1): 381, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36457043

RESUMO

MicroRNAs (miRNAs or miRs) are a class of noncoding single-stranded RNAs that can regulate gene expression by binding to the untranslated sequences at the 3 ' end of messenger RNAs. The microRNA-34 family is dysregulated in various human diseases. It is considered as a tumor-suppressive microRNA because of its synergistic effect with the well-known tumor suppressor p53. As a member of the miRNA-34 family, miR-34b-5p serves as a powerful regulator of a suite of cellular activities, including cell growth, multiplication, development, differentiation, and apoptosis. It promotes or represses disease occurrence and progression by participating in some important signaling pathways. This review aimed to provide an overview and update on the differential expression and function of miR-34b-5p in pathophysiologic processes, especially cancer and injury. Additionally, miR-34b-5p-mediated clinical trials have indicated promising consequences for the therapies of carcinomatosis and injury. With the application of the first tumor-targeted microRNA drug based on miR-34a mimics, it can be inferred that miR-34b-5p may become a crucial factor in the therapy of various diseases. However, further studies on miR-34b-5p should shed light on its involvement in disease pathogenesis and treatment options.

10.
Oxid Med Cell Longev ; 2022: 3415528, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36457728

RESUMO

Background: Long term high fat diets (HFD) promote skin aging pathogenesis, but detailed mechanisms remain unclear especially for inflammaging, which has recently emerged as a pathway correlating aging and age-related disease with inflammation. p16INK4a (hereafter termed p16) inhibits the cell cycle, with p16 deletion significantly inhibiting inflammaging. We observed that HFD-induced p16 overexpression in the skin. Therefore, we investigated if p16 exacerbated inflammaging in HFD-induced skin and also if p16 deletion exerted protective effects against this process. Methods: Eight-week-old double knockout (KO) ApoE-/-p16-/- mice and ApoE-/- littermates were fed HFD for 12 weeks and their skin phenotypes were analyzed. We measured skin fibrosis, senescence-associated secretory phenotype (SASP) levels, and integrin-inflammasome pathway activation using histopathological, RNA-sequencing (RNA-seq), bioinformatics analysis, and molecular techniques. Results: We found that HFD contributed to inflammaging in the skin by activating the NLRP3 inflammasome pathway, increasing inflammatory infiltration, and promoting apoptosis by balancing expression between proapoptotic and antiapoptotic molecules. p16 knockout, when compared with the ApoE-/- phenotype, inhibited skin fibrosis by ameliorating inflammatory infiltration and proinflammatory factor expression (Interleukin-1ß (IL-1ß), Interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)), and also alleviated inflammaging skin progress induced by HFD in the ApoE-/- mouse model. RNA-seq showed that p16 KO mice inhibited both integrin-inflammasome and NF-κB proinflammatory pathway activation. Conclusions: p16 deletion or p16 positive cell clearance could be a novel strategy preventing long term HFD-induced skin aging.


Assuntos
Inibidor p16 de Quinase Dependente de Ciclina , Dieta Hiperlipídica , Dermatopatias , Animais , Camundongos , Inibidor p16 de Quinase Dependente de Ciclina/genética , Dieta Hiperlipídica/efeitos adversos , Fibrose , Inflamassomos , Interleucina-6 , Camundongos Knockout para ApoE , Dermatopatias/genética , Pele/patologia
11.
J Voice ; 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36470823

RESUMO

OBJECTIVES: Describing pronunciation features from multiple perspectives can help doctors accurately diagnose the pathological type of a patient's voice. According to the two modal information of sound signal and electroglottography (EGG) signal, this paper proposes a pathological voice detection and classification algorithm based on multimodal transmission network. METHODS: Firstly, we used the short-time Fourier transform (STFT) to map the features of the two signals, and designed the Mel filter to obtain the Mel spectogram. Then, the constructed multimodal transmission network extracted features from Mel spectogram and applied Multimodal Transfer Module (MMTM) module. Finally, the fusion layer can integrate multimodal information, and the full connection layer diagnoses and classifies voice pathology according to the fused features. RESULTS: The experiment was based on 1179 subjects in Saarbrücken voice database (SVD), and the average accuracy, recall, specificity and F1 score of pathological voice classification reached 98.02%, 98.23%, 97.82% and 97.95% respectively. Compared with other algorithms, the classification accuracy is significantly improved. CONCLUSIONS: The proposed model can integrate multiple modal information to obtain more comprehensive and stable voice features and improve the accuracy of pathological voice classification. Future research will further explore in reducing the time-consuming and complexity of the model.

12.
Indian J Tuberc ; 69(4): 482-495, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36460380

RESUMO

BACKGROUND: Tuberculosis (TB) is a global infectious disease, but there is no ideal vaccine against TB except the Bacille Calmette-Guérin (BCG) vaccine. METHODS: Herein, 25 candidate peptides were predicted from four antigens of Mycobacterium tuberculosis based on their high-affinity binding capacity for the human leukocyte antigen (HLA) DRB1∗0101. Three T-helper 1 (Th1) immunodominant peptides (Ag85B12-26, CFP2112-26, and PPE18149-163) were identified by ELISPOT assays in the humanized C57BL/6 mice. They resulted in a novel Th1 peptide-based vaccine ACP named by the first letter of the three peptides. In addition, the protective efficacy was evaluated in humanized or wild-type C57BL/6 mice and the humoral and cellular immune responses were confirmed in vitro. RESULTS: Compared with the PBS group, the ACP vaccinated mice showed slight decreases in colony-forming units (CFUs) and pathological lesions. However, when using it as a booster, the ACP vaccine did not significantly enhance the protective efficacy of BCG in humanized or wild-type mice. Interestingly, we found that ACP vaccination significantly increased the number of interferon-γ positive (IFN-γ+) T lymphocytes and the levels of IFN-γ cytokines as well as antibodies. Furthermore, the IL-2 level was significantly higher in humanized mice prime-boosted with BCG and ACP. CONCLUSIONS: Our results suggested that ACP vaccination could stimulate higher levels of cytokines and antibodies but failed to improve the protective efficacy of BCG in mice, indicating that the secretion level of IFN-γ may not be positively correlated with the protection efficiency of the vaccine. These findings provided important information on the feasibility of a peptide vaccine as a booster for enhancing the protective efficacy of BCG.


Assuntos
Mycobacterium tuberculosis , Tuberculose dos Linfonodos , Humanos , Camundongos , Animais , Camundongos Endogâmicos C57BL , Vacina BCG , Vacinas de Subunidades , Interferon gama , Citocinas
13.
Plant Physiol ; 2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36461917

RESUMO

Biphasic production of reactive oxygen species (ROS) has been observed in plants treated with avirulent bacterial strains. The first transient peak corresponds to PTI (pattern-triggered immunity)-ROS, whereas the second long-lasting peak corresponds to ETI (effector-triggered immunity)-ROS. PTI-ROS are produced in the apoplast by plasma membrane-localized NADPH oxidases, and the recognition of an avirulent effector increases the PTI-ROS regulatory module, leading to ETI-ROS accumulation in the apoplast. However, how apoplastic ETI-ROS signaling is relayed to the cytosol is still unknown. Here, we found that in the absence of cytosolic ascorbate peroxidase 1 (APX1), the second phase of ETI-ROS accumulation was undetectable in Arabidopsis (Arabidopsis thaliana) using luminol-based assays. In addition to being a scavenger of cytosolic H2O2, we discovered that APX1 served as a catalyst in this chemiluminescence ROS assay by employing luminol as an electron donor. A horseradish peroxidase (HRP)-mimicking APX1 mutation (APX1W41F) further enhanced its catalytic activity toward luminol, whereas an HRP-dead APX1 mutation (APX1R38H) reduced its luminol oxidation activity. The cytosolic localization of APX1 implies that ETI-ROS might accumulate in the cytosol. When ROS were detected using a fluorescent dye, green fluorescence was observed in the cytosol 6 h after infiltration with an avirulent bacterial strain. Collectively, these results indicate that ETI-ROS eventually accumulate in the cytosol, and cytosolic APX1 catalyzes luminol oxidation and allows monitoring of the kinetics of ETI-ROS in the cytosol. Our study provides important insights into the spatial dynamics of ROS accumulation in plant immunity.

14.
Hortic Res ; 9: uhac207, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467273

RESUMO

Production of reactive oxygen species (ROS) is an important immune response in plant multilayer defense mechanisms; however, direct modification of ROS homeostasis to breed plants with broad-spectrum resistance to disease has not yet been successful. In Arabidopsis, the receptor-like cytosolic kinase AtRIPK regulates broad-spectrum ROS signaling in multiple layers of the plant immune system. Upon treatment with immune elicitors, AtRIPK is activated and phosphorylates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which leads to ROS production. In this study, we identified an AtRIPK ortholog in tomatoes and generated knockdown mutants using CRISPR/Cas9 technology. Slripk mutants displayed reduced ROS production in response to representative immune elicitors and were susceptible to pathogenic bacteria and fungi from different genera, including Ralstonia solanacearum, Pectobacterium carotovorum, Botrytis cinerea, and Fusarium oxysporum, which are leaf and root pathogens with hemibiotrophic and necrotrophic infection strategies. In contrast, transgenic tomato plants overexpressing SlRIPK are more resistant to these pathogens. Remarkably, the slripk mutants and SlRIPK-overexpressing transgenic plants did not exhibit significant growth retardation or yield loss. These results suggest that overexpression of SlRIPK confers broad-spectrum disease resistance without a yield penalty in tomato plants. Our findings suggest that modifying ROS homeostasis by altering the regulatory components of ROS production in plant immunity could contribute to engineering or breeding broad-spectrum disease-resistant crops without yield penalty.

15.
Hortic Res ; 9: uhac198, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467272

RESUMO

Drought stress caused by water deficit reduces plant productivity in many regions of the world. In plants, basic helix-loop-helix (bHLH) transcription factors regulate a wide range of cellular activities related to growth, development and stress response; however, the role of tomato SlbHLHs in drought stress responses remains elusive. Here, we used reverse genetics approaches to reveal the function of SlbHLH96, which is induced by drought and abscisic acid (ABA) treatment. We found that SlbHLH96 functions as a positive regulator of drought tolerance in tomato. Overexpression of SlbHLH96 in tomato improves drought tolerance by stimulating the expression of genes encoding antioxidants, ABA signaling molecules and stress-related proteins. In contrast, silencing of SlbHLH96 in tomato reduces drought tolerance. SlbHLH96 physically interacts with an ethylene-responsive factor, SlERF4, and silencing of SlERF4 in tomato also decreases drought tolerance. Furthermore, SlbHLH96 can repress the expression of the ABA catabolic gene, SlCYP707A2, through direct binding to its promoter. Our results uncover a novel mechanism of SlbHLH96-mediated drought tolerance in tomato plants, which can be exploited for breeding drought-resilient crops.

16.
Sensors (Basel) ; 22(23)2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36501961

RESUMO

The two-stage suspension system (TSSS) is designed for the fiber optic vector hydrophone (FOVH) to isolate the vibration from the mooring rope. The acceleration transmissibility of the TSSS is studied theoretically and experimentally. The results show that the TSSS has a major advantage over the traditional one-stage suspension system (OSSS). Typically, the vibration isolation of the TSSS is demonstrated to be over 25 dB higher than that of the OSSS at 100 Hz. Meanwhile, it is demonstrated that the TSSS has little negative influence on the in-band acceleration response of the FOVH. The TSSS has the prospect of reducing the mechanical noise of the FOVH, which is conducive to suppressing the self-noise and enhancing the ability of weak signal detection.

17.
J Chem Phys ; 157(23): 234109, 2022 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-36550029

RESUMO

In this study, based on the theory developed in Paper I, we explore the combined effects of molecular fluorescence and excitation energy transfer in a minimal model-a pair of single-vibration-mode chromophores coupled to surface plasmon polaritons. For the chromophores with zero Huang-Rhys factors and strong couplings to surface plasmon polaritons, we find that the frequencies of Rabi oscillations (the strengths of strong light-matter couplings) are associated with the initial excitation conditions. On the other hand, for the chromophores weakly coupled to surface plasmon polaritons, our numerical calculations together with analytical analysis elaborate on the conditions for the superradiant and subradiant decay behaviors. Moreover, we show that the modified decay rate constants can be explicitly expressed in terms of generalized spectral densities (or dyadic Green's functions), revealing a relationship between photonic environments and the collective effects such as superradiance and subradiance. For the chromophores with nonzero Huang-Rhys factors and strong coupling to surface plasmon polaritons, the effects of molecular vibrations emerge. We demonstrate that the low-frequency vibrational modes do not affect the excited state population dynamics, while the high-frequency vibrational modes can modify either the period of Rabi oscillation (Franck-Condon Rabi oscillation) or the amplitude of excited state population. Our study shows that the collective effects, including superradiance and subradiance, can be controlled via dielectric environments and initial excitation conditions, providing new insights into polariton chemistry and the design of quantum optical devices.

18.
Front Microbiol ; 13: 1071530, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36560956

RESUMO

Burkholderia arboris, which belongs to the Burkholderia cepacia complex, has been shown to possess antifungal activity against several plant fungal pathogens; however, the antifungal compounds are yet to be identified. Here, we identified the antifungal compounds produced by B. arboris using genetic and metabolomic approaches. We generated a Tn5 transposon mutation library of 3,000 B. arboris mutants and isolated three mutants with reduced antifungal activity against the plant fungal pathogen Fusarium oxysporum. Among the mutants, the M464 mutant exhibited the weakest antifungal activity. In the M464 genome, the transposon was inserted into the cobA gene, encoding uroporphyrin-III methyltransferase. Deletion of the cobA gene also resulted in reduced antifungal activity, indicating that the cobA gene contributed to the antifungal activity of B. arboris. Furthermore, a comparison of the differential metabolites between wild type B. arboris and the ∆cobA mutant showed a significantly decreased level of tetrapeptide His-Ala-Phe-Lys (Hafk) in the ∆cobA mutant. Therefore, a Hafk peptide with D-amino acid residues was synthesized and its antifungal activity was evaluated. Notably, the Hafk peptide displayed significant antifungal activity against F. oxysporum and Botrytis cinerea, two plant pathogens that cause destructive fungal diseases. Overall, a novel antifungal compound (Hafk) that can be used for the biocontrol of fungal diseases in plants was identified in B. arboris.

19.
BMC Endocr Disord ; 22(1): 317, 2022 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-36514041

RESUMO

IFN-γ is critical for both thyroid and ovarian function, while thyroxine, secreted from the thyroid gland, regulates the ovarian function via the hypothalamus-pituitary -ovary axis. However, the effect of thyroxine on INF-γ involved in the regulation of hypothalamic pituitary ovarian axis ovarian function is hitherto unknown. Therefore, we set up three groups including a sham-operated group, an experimental thyroidectomized group, and an experimental thyroidectomized group treated with T4 to reveal the IFN-γ expression levels in the in the hypothalamus, pituitary gland, and ovary by immunohistochemical staining, RT-PCR, and Western blotting. IFN-γ-like immunoreactive-positive substances were visualized in the hypothalamus, pituitary gland, and ovary, which were located mainly in the cytoplasm of the hypothalamic neurons anterior pituitary cells, luteal cells, and theca cells in the ovary of hypothyroidism rats, respectively. RT-PCR and Western blotting showed that the rats in the experimental thyroidectomized group treated with T4 had significantly elevated expression of IFN-γ at both the mRNA and protein levels. Thyroxine affects the expression of IFN-γ in the thalamus-pituitary-ovarian axis, which may influence the secretion of IFN-γ to regulate ovarian function during hypothyroidism. This work highlights the potential effect of thyroxine on the involvement of INF-γ in the modulation of the ovarian function in the hypothalamic-pituitary-ovarian axis.


Assuntos
Hipotireoidismo , Tiroxina , Feminino , Animais , Ratos , Tiroxina/farmacologia , Ovário/metabolismo , Hipófise/metabolismo , Hipotireoidismo/metabolismo , Hipotálamo/metabolismo
20.
Front Med (Lausanne) ; 9: 1055330, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36523777

RESUMO

Background: Pulmonary hypertension (PH) is a common complication of end-stage renal disease which is associated with adverse outcomes including all-cause mortality and cardiovascular events. Recent studies have demonstrated that Sacubitril/Valsartan (Sac/Val) as an enkephalinase inhibitor and angiotensin II receptor blocker could reduce pulmonary artery systolic pressure (PASP) and improve the prognosis of patients with heart failure. However, whether Sac/Val is effective in hemodialysis (HD) patients with PH is essentially unknown. In this retrospective study, we aimed to evaluate the efficacy and safety of Sac/Val in the treatment of PH in HD patients. Methods: A total of 122 HD patients with PH were divided into Sac/Val group (n = 71) and ARBs group (n = 51) based on the treatment regimen. The PASP, other cardiac parameters measured by echocardiography, and cardiac biomarkers including N-terminal fragment of BNP (NT-proBNP) and cardiac troponin I (cTnI) were observed at baseline and 3 months after treatment. Results: There were no significant differences in the baseline characteristics between the two groups. PASP decreased significantly from 45(38, 54) to 28(21, 40) mmHg in Sac/Val group (p < 0.001). PASP reduced from 41(37, 51) to 34(27, 44) mmHg in ARBs group (p < 0.001), and the decrease was more pronounced in the Sac/Val group (p < 0.001). In addition, improvements in the right atrial diameter (RAD), left ventricular diameter (LVD), left ventricular posterior wall thickness (LVPWT), left atrial diameter (LAD), pulmonary artery diameter (PAD), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular ejection fraction (LVEF), and fractional shortening (FS) were found in Sac/Val group (ps < 0.05). After 3 months, LVD, LAD, LVEDV, LVESV, LVEF, SV, and PASP were significantly improved in Sac/Val group compared with ARBs group (ps <0.05). Significant reduction in NT-proBNP [35,000 (15,000, 70,000) pg/ml vs. 7,042 (3,126, 29,060) pg/ml, p < 0.001] and cTnI [0.056(0.031, 0.085) ng/ml vs. 0.036 (0.012, 0.056) ng/ml, p < 0.001) were observed in Sac/Val group. No significant differences were observed in adverse events between the two groups (ps > 0.05). Conclusion: Sac/Val seems to be an efficacious regimen in PH with favorable safety and has huge prospects for treating PH in HD patients.

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