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1.
Mol Phylogenet Evol ; 142: 106641, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31605813

RESUMO

The family Caprifoliaceae s.l. is an asterid angiosperm clade of ca. 960 species, most of which are distributed in temperate regions of the northern hemisphere. Recent studies show that the family comprises seven major clades: Linnaeoideae, Zabelia, Morinoideae, Dipsacoideae, Valerianoideae, Caprifolioideae, and Diervilloideae. However, its phylogeny at the subfamily or genus level remains controversial, and the backbone relationships among subfamilies are incompletely resolved. In this study, we utilized complete plastome sequencing to resolve the relationships among the subfamilies of the Caprifoliaceae s.l. and clarify several long-standing controversies. We generated and analyzed plastomes of 48 accessions of Caprifoliaceae s.l., representing 44 species, six subfamilies and one genus. Combined with available Caprifoliaceae s.l. plastomes on GenBank and 12 outgroups, we analyzed a final dataset of 68 accessions. Genome structure was strongly conserved in general, although the boundaries between the Inverted Repeat were found to have contracted across Caprifoliaceae s.l. to exclude rpl2, rps19, and ycf1, all or parts of which are typically present in the IR of most angiosperms. The ndhF gene was found to have been inverted in all plastomes of Adoxaceae. Phylogenomic analyses of 68 complete plastomes yielded a highly supported topology that strongly supported the monophyly of Zabelia and its sister relationship to Morinoideae. Moreover, a clade of Valerianoideae + Dipsacoideae was recovered as sister to a clade of Linnaeoideae + Zabelia + Morinoideae clade, and Heptacodium was sister to remaining Caprifolioideae. The Diervilloideae and Caprifolioideae were successively sister to all other Caprifoliaceae s.l. Major lineages of Caprifoliaceae s.l. were estimated to have diverged from the Upper Cretaceous to the Eocene (50-100 Ma), whereas within-genus diversification was dated to the Oligocene and later, concomitant with global cooling and drying. Our results demonstrate the power of plastid phylogenomics in improving estimates of phylogeny among genera and subfamilies, and provide new insights into plastome evolution across Caprifoliaceae s.l.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117409, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31357050

RESUMO

A novel colorimetric and fluorescence turn-on chemosensor TBB with benzothiazolium moiety has been explored, which exhibited the high selectivity for cyanide ion (CN-) in THF-H2O (2:8, v/v) mixture. The aqueous solution of sensor TBB was scarcely emissive. In the presence of CN- ion, the nucleophilic addition of CN- with the benzothiazolium CN bond of TBB produced the new species TBB-CN, consequently resulting in the intense orange-red emission by aggregation-induced emission (AIE) effect. Meanwhile, the color of solution was changed from orange-yellow to light yellow. The sensing mechanism was verified by Mass spectrometry, NMR analysis and DFT calculations.

3.
Nat Prod Res ; : 1-9, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31674853

RESUMO

Butyrolactone I, one of the major secondary metabolites of fungus Aspergillus terreus, is a selective cdc2 kinase inhibitor. In the present study, the metabolism of butyrolactone I in male Wistar rats was investigated by characterising metabolites excreted into faeces. Following an oral dose of 40 mg/kg butyrolactone I, two phase I metabolites were isolated from the faeces of rat. The new structure was identified on the spectroscopic data analysis. The new compound V1 and known compound V2 were tested for their cytotoxicity, antimicrobial and antioxidant activity. V1 and V2 showed significant free radical scavenging ability. V2 also showed strong inhibitory activity against Staphylococcus aureus.

4.
Life Sci ; 239: 116875, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31676235

RESUMO

Ubiquitin (Ub) is a small protein that can be attached to substrate proteins to direct their degradation via the proteasome. Deubiquitinating enzymes (DUBs) reverse this process by removing ubiquitin from its substrate protein. Over the past few decades, ubiquitin-specific protease 14 (USP14), a member of the DUBs, has emerged as an important player in various types of cancers. In this article, we review and summarize biological function of USP14 in tumorigenesis and multiple signaling pathways. To determine its role in cancer, we analyzed USP14 gene expression across a panel of tumors, and discussed that it could serve as a novel bio-marker in several types of cancer. And recent contributions indicated that USP14 has been shown to act as a tumor-promoting gene via the AKT, NF-κB, MAPK pathways etc. Besides, drugs targeting USP14 have shown potential anti-tumor effect and clinical significance. We focus on recent studies that explore the link between USP14 and cancer, and further discuss USP14 as a novel target for cancer therapy.

5.
J Genet Genomics ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31776062

RESUMO

The functional heterogeneity of hematopoietic stem cells (HSCs) has been comprehensively investigated by single-cell transplantation assay. However, the heterogeneity regarding their physiological contribution remains an open question, especially for those with life-long hematopoietic fate of rigorous self-renewing and balanced differentiation capacities. In this study, we revealed that Procr expression was detected principally in phenotypical vascular endothelium co-expressing Dll4 and CD44 in the mid-gestation mouse embryos, and could enrich all the HSCs of the embryonic day 11.5 (E11.5) aorta-gonad-mesonephros (AGM) region. We then used a temporally restricted genetic tracing strategy to irreversibly label the Procr-expressing cells at E9.5. Interestingly, most labeled mature HSCs in multiple sites (such as AGM) around E11.5 were functionally categorized as lymphomyeloid-balanced HSCs assessed by direct transplantation. Furthermore, the labeled cells contributed to an average of 7.8% of immunophenotypically defined HSCs in E14.5 fetal liver (FL) and 6.9% of leukocytes in peripheral blood (PB) during one-year follow-up. Surprisingly, in aged mice of 24 months, the embryonically tagged cells displayed constant contribution to leukocytes with no bias to myeloid or lymphoid lineages. Altogether, we demonstrated, for the first time, the existence of a subtype of physiologically long-lived balanced HSCs as hypothesized, whose precise embryonic origin and molecular identity await further characterization.

6.
Bioresour Technol ; : 122409, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31740246

RESUMO

In this study, the growth and lipid accumulation of Scenedesmus sp. using different nanoparticles and light sources were investigated. Xenon lamp can produce a broad illumination spectrum, and exhibited better performance than light-emitting diode. SiC and g-C3N4 nanoparticles improved the biomass and lipid accumulation, whereas TiO2 and TiC nanoparticles had inhibitory influence on microalgae. Lipid production can be improved by oxidative stress produced by combination of nanoparticles and xenon lamp irradiation. At the optimal SiC nanoparticles concentration of 150 mg L-1 and photoperiod of 6:18 h, the maximum biomass concentration and total lipid content reached 3.18 g L-1 and 40.26%, respectively. The addition of SiC nanoparticles could promote the substrate utilization rate and induce stress condition, thereby enhancing the activity of acetyl-CoA carboxylase and lipid biosynthesis. This research shows that SiC nanoparticles addition combined with xenon lamp illumination is a promising strategy to promote microalgal growth and lipid accumulation.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31742987

RESUMO

Porous colloidal hollow spheres have been applied to diversified fields over the past few decades. However, developing simple and efficient methods to prepare such porous hollow spheres with macro pores remains a challenge. To address this problem, we present a patchy templated synthesis route, which can be used to prepare such colloidal hollow spheres that have macro pores through the shells. This was achieved by using patchy poly(styrene-co-sodium styrene sulphonate) (poly(St-NaSS)) spheres as the template and poly(allylamine hydrochloride) as binding molecules. SiO2 can site-selectively only grow on one kind of patches, resulting in the formation of porous hollow spheres. The pore sizes can be tuned from about 50 to 400 nm. The resulting porous hollow spheres have a Janus character so that Au NPs can only be attached to the interior surfaces in situ, which can be used as catalytic microreactors and show the catalytic performance of pore size dependence.

8.
Blood ; 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31697805

RESUMO

Along with the aorta-gonad-mesonephros region, the head is a site of hematopoietic stem and progenitor cell (HS/PC) development in the mouse embryo. Macrophages are present in both these embryonic hemogenic sites and recent studies indicate a functional interaction of macrophages with hematopoietic cells as they are generated in the aorta. Whereas, brain macrophages or 'microglia' are known to affect neuronal patterning and vascular circuitry in the embryonic brain, it is unknown whether macrophages play a role in head hematopoiesis. Here we characterize head macrophages and examine whether they affect the HS/PC output of the hindbrain-branchial arch (HBA) region of the mouse embryo. We show that HBA macrophages are CD45+F4/80+CD11b+Gr1- and express the macrophage-specific Csf1r-GFP reporter. In the HBA of chemokine receptor deficient (Cx3cr1-/-) embryos, a reduction in erythropoiesis is concomitant with a decrease in HBA macrophage percentages. In cocultures, we show that head macrophages boost HPC numbers from HBA endothelial cells >2-fold, and that the pro-inflammatory factor TNFα is produced by head macrophages and influences HBA hematopoiesis in vitro. Taken together, head macrophages play a positive role in HBA erythropoiesis and HS/PC expansion and/or maturation, acting as micro-environmental cellular regulators in hematopoietic development.

9.
Math Biosci Eng ; 16(6): 7112-7125, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31698606

RESUMO

Long noncoding RNAs (lncRNA) play important roles in gene expression regulation in diverse biological contexts. Numerous studies have indicated that lncRNA-gene interactions are closely related to the occurrence and development of cancers. Thus, it is important to develop an effective method for the identification of target genes of lncRNA. Meanwhile, the high throughput sequencing data provide tremendous information about regulation correlation, by which the new target genes could be detected from known lncRNA regulated genes. In this study, we developed a method for elucidating lncRNA-gene interactions by using a biclustering approach, which allows for the identification of particular expression patterns across multiple datasets, indicating networks of lncRNA and gene interactions. A p-value strategy is followed to link co-expression patterns to certain lncRNAs. The method was applied on the breast cancer RNA-seq datasets along with a set of known lncRNA regulated genes. The evaluation indicated that the method can detect some new targets but fail to obtain higher coverage. We believe that this developed method will provide useful information for future studies on lncRNAs.

10.
Cell Res ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729468

RESUMO

Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells (PSCs) is a central aim in T cell regenerative medicine. To date, preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge. Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme (iR9-PSC) in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients. Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs, unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors. The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαß repertoire. The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice. Furthermore, gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells. This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source.

11.
Sci Total Environ ; 700: 134536, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31689651

RESUMO

Heterogeneous electro-Fenton (EF) technology has been wildly applied for the treatment of wastewater containing dyes and other organic pollutants. However, biologically treatment should be further applied after heterogeneous electro-Fenton process in order get better effluent quality. In the present study, a simple electropolymerization method using poly (3,4-ethylenedioxythiophene) (PEDOT) and graphene oxide (GO) was applied for graphite felt (GF) electrode modification as cathode in EF system, and coupling subsequently aerobic granular sludge (AGS) biological treatment for dye wastewater treatment. The modified electrode was characterized by scanning electron microscopy (SEM), Raman spectrum, and cyclic voltammetry (CV). Data implied that much higher H2O2 productivity, current response and coulomb efficiency (CE) were achieved by using GO/PEDOT:NaPSS modified GF. The results could be ascribed to the synergistic effect between PEDOT and GO that accelerated the electron transfer rate. Moreover, the H2O2 production capacity remained over 84.2% after 10-times reuses for GO/PEDOT:NaPSS modified GF, indicating that GO significantly improved the stability and life of electrode. Compared with the single system, the total organic carbon (TOC) and chemical oxygen demand (COD) removal efficiencies of the combined system degradation methylene blue (MB) wastewater were significantly improved. Therefore, this modified GF could be used as a potentially useful cathode in heterogeneous EF technology for actual wastewater treatment and the combined system have a promising engineering application value in MB wastewater degradation field.

12.
J Neurochem ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31705656

RESUMO

Sevoflurane is one of the most widely used anesthetics with recent concerns rising about its pediatric application. The synaptic toxicity and mechanisms underlying its long term cognition impairment remain unclear. In the present study, we investigated the expression and roles of homeodomain interacting protein kinase 2 (HIPK2), a stress activating kinase involved in neuronal survival and synaptic plasticity, and its downstream JNK/c-Jun signaling in the long term toxicity of neonatal Sevoflurane exposure. Our data showed that neonatal Sevoflurane exposure results in impairment of memory, enhancement of anxiety, less number of excitatory synapses and lower levels of synaptic proteins in the hippocampus of adult rats without significant changes of hippocampal neuron numbers. Up-regulation of HIPK2 and JNK/c-Jun was observed in hippocampal granular neurons shortly after Sevoflurane exposure and persisted to adult. A64, antagonist of HIPK2, could significantly rescue the cognition impairment, decrease of long term potentiation, reduction of spine density and activation of JNK/c-Jun induced by Sevoflurane. JNK antagonist SP600125 partially restored synapse development and cognitive function without affecting the expression of HIPK2. These data, in together, revealed a novel role of HIPK2-JNK/c-Jun signaling in the long term synaptic toxicity and cognition impairment of neonatal Sevoflurane exposure, indicating HIPK2-JNK/c-Jun cascade as a potential target for reducing the synaptic toxicity of Sevoflurane.

13.
Bioengineered ; 10(1): 679-688, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31679450

RESUMO

MiR-25 is a well-documented oncogenic miRNA implicated in esophageal squamous cell carcinoma (ESCC) development, progression and metastasis. However, whether and how miR-25 is involved in the development and metastasis of ESCC remain un-addressed. By using qRT-PCR analysis to compare levels of miR-25 in ESCC tissues with or without lymph node metastasis (LNM), it showed that ESCC tissues with LNM had increased levels of miR-25, which was correlated with tumor metastasis and poor prognosis. Gain- and loss-of-function assays indicated that targeting miR-25 could reverse EMT, and reduce in vitro cell migration and invasion, but not apoptosis and proliferation of ESCC. Furthermore, targeting miR-25 inhibited in vivo lung metastasis, and vice versa. And E-cadherin was a direct target of miR-25 through which affected EMT process and metastasis of ESCC. It is therefore indicated that miR-25 promotes metastasis of ESCC through E-cadherin and EMT events, thus may serves as a negative prognostic factor and possible target for treatment of ESCC patients.

14.
Zhongguo Zhen Jiu ; 39(11): 1239-43, 2019 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-31724364

RESUMO

Under the revised demand of theoretic development in clinical practice and scientific research of acupuncture and moxibustion, the reconstruction of the theory was discussed on the "three yin and three yang" subarea division in the body and acupuncture effect in line with the traditional thinking of Chinese medicine so as to explore the longitudinal foundation and rule in the human body for the "effective stimulation". ①Based on the theory of "qi-space correlation" in Internal Classic and the source of nomenclature of "three yin and three yang", the body is divided in stereo zone in accordance with the theory of "three yin and three yang". ②In view of theoretical reasoning and practical interpretation, it is expounded that the same longitudinal stereo zone subarea in the body could respond to diseases and generate therapeutic effects. ③It is pointed out that the subareas of "three yin and three yang" in the body are closely related to meridians and collaterals, which is the basis of the spatial structure and and physical effect. The importance of the "body" itself is ignored nowadays because of the conventional thinking of "qi and blood" in traditional Chinese medicine.


Assuntos
Terapia por Acupuntura , Acupuntura , Meridianos , Corpo Humano , Humanos , Medicina Tradicional Chinesa , Yin-Yang
15.
Stem Cell Res Ther ; 10(1): 344, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31753016

RESUMO

With the increasing incidence of cartilage-related diseases such as osteoarthritis (OA) and intervertebral disc degeneration (IDD), heavier financial and social burdens need to be faced. Unfortunately, there is no satisfactory clinical method to target the pathophysiology of cartilage-related diseases. Many gene expressions, signaling pathways, and biomechanical dysregulations were involved in cartilage development, degeneration, and regeneration. However, the underlying mechanism was not clearly understood. Recently, lots of long non-coding RNAs (lncRNAs) were identified in the biological processes, including cartilage development, degeneration, and regeneration. It is clear that lncRNAs were important in regulating gene expression and maintaining chondrocyte phenotypes and homeostasis. In this review, we summarize the recent researches studying lncRNAs' expression and function in cartilage development, degeneration, and regeneration and illustrate the potential mechanism of how they act in the pathologic process. With continued efforts, regulating lncRNA expression in the cartilage regeneration may be a promising biological treatment approach.

16.
J Craniofac Surg ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31764559

RESUMO

This study aimed to evaluate 2 advanced methods for presurgical calculation of bilateral alveolar cleft defect volume using 3-dimensional (3D) printing and computer-aided engineering (CAE) software. Twelve patients with bilateral alveolar cleft (4 females, 8 males, age range 8-11 years) were enrolled in the study. All patients underwent preoperative helical computed tomography scans, and the digital imaging and communications in medicine (DICOM) data of the computed tomography images were analyzed using 3D printing and CAE software. The 3D-printed model used a simulated graft, and graft volume was measured by water displacement. The CAE software used a subtractive method to calculate volume. Simulated graft volumes and CAE-software-derived volumes were compared using Pearson's correlation test and paired Student's t-test. The average volume of the simulated bone grafts obtained using 3D-printed models was 1.45 mL, which was lower than the CAE-software-derived mean volume of 1.52 mL. The mean calculation time using the 3D-printed model was 51.5 minutes, which was 32.1 minutes longer than the average 19.4 minutes when using CAE software. Paired Student's t-test revealed no statistically significant difference between the volumes derived from these methods. The results of this study further validated the use of 3D printing and CAE technique in bilateral alveolar defect repair. The CAE software is more cost-effective in presurgical volumetric assessment. Moreover, 3D models overcome limitations of 3D visualizations by providing tactile feedback and superior appreciation of visuospatial relationships among anatomical structures.

17.
Biomol NMR Assign ; 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31707562

RESUMO

Phage study draws more attention recently as the bacterial antibiotic resistances become a major threat for global health. Bacteriophage T4 is one of the most studied the phages and the representative of Tevenvirinae subfamily. Since 1950s, T4 phage has been studied more intensively than any other large lytic phages and its biological studies have provided basis for current phage biology as well as other applications. However, among approximately 300 T4 genes, 130 of them still remain uncharacterized. Coded by y04L gene in pin-nrdC intergenic region, Y04L is an example of such proteins whose biological function and mechanism are yet to be addressed. While Pin blocks bacterial Lon protease and thus inhibits bacterial toxin-antitoxin system, NrdC or Glutaredoxin is a specific reducing agent for the phage-induced ribonucleotide reductase. With two interesting neighbouring genes, this 11.9 kDa protein may be functionally related to Pin or NrdC. Here, using solution-state NMR, our near-complete resonance assignment of Y04L provides a basis for future structure determination and further mechanism study.

19.
Sci Rep ; 9(1): 15836, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31676842

RESUMO

Aphid-parasitoid interactions have been widely used as a model system in research studies on the structure and functions of arthropod food web. Research on aphid-parasitoid food webs is hindered by their micromorphological characteristics and the high amount of labor associated with their development. Species-specific primers for cotton aphids and their parasitoids were designed and integrated into two multiplex PCRs and six singleplex PCRs, and all PCRs were optimized to achieve high specificity and sensitivity (100-10,000 DNA copies). One cotton aphid (Aphis gossypii) as well as three primary parasitoid and seven hyperparasitoid species or genera were detected using this molecular approach. This group comprises all the primary parasitoids and 97.2-99.6% of the hyperparasitoids reported in cotton fields in northern China. A tritrophic aphid-primary parasitoid-hyperparasitoid food web was then established. The described method constitutes an efficient tool for quantitatively describing the aphid-primary parasitoid-hyperparasitoid food webs and assessing the efficiency of the biological control of parasitoids in cotton fields in northern China.

20.
J Exp Clin Cancer Res ; 38(1): 455, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694696

RESUMO

BACKGROUND: The regulatory non-coding RNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), emerge as pivotal markers during tumor progression. Abnormal sialylated glycoprotein often leads to the malignancy of colorectal cancer (CRC). METHODS: Differential levels of HOTAIR and ST6GAL1 are analyzed by qRT-PCR. Functionally, CRC cell proliferation, aggressiveness and apoptosis are measured through relevant experiments, including CCK8 assay, colony formation assay, transwell assay, western blot and flow cytometry. Dual-luciferase reporter gene assay and RIP assay confirm the direct interaction between HOTAIR and miR-214. The lung metastasis, liver metatstasis and xenografts nude mice models are established to show the in vivo effect of HOATIR. RESULTS: Here, differential levels of HOTAIR and ST6GAL1 are primarily observed in CRC samples and cells. Upregulated HOTAIR and ST6GAL1 are crucial predictors for poor CRC prognosis. Altered level of ST6GAL1 modulates CRC malignancy. Furthermore, ST6GAL1 and HOTAIR are confirmed as the direct targets of miR-214, and ST6GAL1 is regulated by HOTAIR via sponging miR-214. ST6GAL1 induces the elevated metabolic sialylation of c-Met, which is co-mediated by HOTAIR and miR-214. Sialylated c-Met affects the activity of JAK2/STAT3 pathway. The regulatory role of HOTAIR/miR-214/ST6GAL1 axis also impacts CRC procession. In addition, HOTAIR mediates lung metastasis, liver metastasis and tumorigenesis in vivo. ShHOTAIR and AMG-208 are combined to inhibit tumorigenesis for successful drug development. CONCLUSION: The HOTAIR/miR-214/ST6GAL1 axis commands the CRC malignancy by modifying c-Met with sialylation and activating JAK2/STAT3 pathway. Our study presents novel insights into CRC progression and provided prospective therapeutic target for CRC.

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