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1.
Sens Actuators B Chem ; 374: 132800, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36213178

RESUMO

Rapid, convenient and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is urgently needed to timely diagnosis of coronavirus pandemic (COVID-19) and control of the epidemic. In this study, a signal-off photoelectrochemical (PEC) immunosensor was constructed for SARS-CoV-2 nucleocapsid (N) protein detection based on a magnetic all-solid-state Z-scheme heterojunction (Fe3O4@SiO2@TiO2@CdS/Au, FSTCA). Integrating the advantages of magnetic materials and all-solid-state Z-scheme heterostructures, FSTCA was implemented to ligate the capture antibody to form magnetic capture probe (FSTCA/Ab1). It can simplify the separation and washing process to improve reproducibility and stability, while allowing immune recognition to be performed in the liquid phase instead of the traditional solid-liquid interface to improve anti-interference. Besides, the heterojunction inhibited the recombination of photogenerated electron/hole (e-/h+) and promoted the light absorption to provide superior photoelectric substrate signal. The mechanism of photogenerated e-/h+ transfer of FSTCA were investigated by the electron spin resonance (ESR) spectroscopy. SiO2 spheres loaded with Au NPs utilized as an efficient signal quencher. The steric hindrance effect of SiO2@Au labeled detection antibodies (SiO2@Au-Ab2) conjugates significantly diminished light absorption and hindered the transfer of photogenerated electrons, further amplifying the signal change value. Based on the above merits, the elaborated immunosensor had a wide linear range of 10 pg mL-1-100 ng mL-1 and a low detection limit down to 2.9 pg mL-1 (S/N = 3). The fabricated PEC immunosensor demonstrated strong anti-interference, easy operation, and high sensitivity, showing enormous potential in clinical diagnosis of SARS-CoV-2.

2.
Sci Total Environ ; 856(Pt 1): 158977, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155040

RESUMO

Nitrogen and phosphorus contamination in wastewater is a serious environmental concern and poses a global threat to sustainable development. In this paper, a comprehensive review of the studies on simultaneous nitrogen and phosphorus removal (SNPR) during 1986-2022 (538 publications) was conducted using bibliometrics, which showed that simultaneous nitrification, denitrification, and phosphorus removal (SNDPR) is the most promising process. To better understand SNDPR, the dissolved oxygen, carbon to nitrogen ratio, carbon source type, sludge retention time, Cu2+ and Fe3+, pH, salinity, electron acceptor type of denitrifying phosphorus-accumulating organisms (DPAOs), temperature, and other influencing factors were analyzed. Currently, SNDPR has been successfully implemented in activated sludge systems, aerobic granular sludge systems, biofilm systems, and constructed wetlands; sequential batch mode of operation is a common means to achieve this process. SNDPR exhibits a significant potential for phosphorus recovery. Future research needs to focus on: (1) balancing the competitiveness between denitrifying glycogen-accumulating organisms (DGAOs) and DPAOs, and countermeasures to deal with the effects of adverse conditions on SNDPR performance; (2) achieving SNDPR in continuous flow operation; and (3) maximizing the recovery of P during SNDPR to achieve resource sustainability. Overall, this study provides systematic and valuable information for deeper insights into SNDPR, which can help in further research.


Assuntos
Nitrificação , Fósforo , Esgotos , Desnitrificação , Eliminação de Resíduos Líquidos , Reatores Biológicos , Nitrogênio , Carbono
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121841, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36179565

RESUMO

Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy was employed to discriminate protein samples comprising bovine serum albumin, neurotensin, ovalbumin, ricin, trypsin from bovine pancreas and trypsin from porcine pancreas. Two methods of feature extraction with and without parameterization were applied to the spectral data in order to evaluate their performance of discrimination between protein samples. The discrimination of protein samples was conducted by k-means clustering algorithm and eigenvalue extracting procedure based on principal component analysis (PCA). It was found that the method of feature extraction without parameterization performed best, correctly attributing 100% of the spectral data in the condition of two principal components (PCs) captured. Features extracted with spectral parameterization failed to separate ricin and trypsin from bovine pancreas in same condition. Without spectral parameterization, less dimensionality and unique principal components captured by PCA indicates the spectrally-resolved features of corresponding protein samples. By clustering using each spectrum at fixed excitation wavelength, excitation wavelengths matched with common intrinsic fluorophores were found to be more sensitive to the classification accuracy. Contributions of spectral features extracted from EEM to the principal components were discussed and demonstrated their feature differentiation capabilities among six protein samples. These results reveal that appropriate extraction approach of features in combination with PCA analysis could be used in discrimination of protein samples at species level as a spectroscopic diagnostic tool. Our study provides fundamental references about computational strategies when EEM are used to explore proteins in ambient environment.


Assuntos
Ricina , Espectrometria de Fluorescência/métodos , Tripsina , Análise de Componente Principal , Análise por Conglomerados
4.
Talanta ; 252: 123840, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36007295

RESUMO

Currently, many aptamers of different target molecules have been screened by systematic evolution of ligands by exponential enrichment (SELEX) method. However, since the crystal structure of aptamers is complicated to obtain, few studies focus on the quantitative structure activity relationship (QSAR) of aptamers. It is significant to develop a virtual screening method for aptamers based on the QSAR model. In this study, a piezoresistive micro-cantilever aptasensor was fabricated to quantificationally detect ricin based on a new aptamer obtained via a virtual screening method. The advanced screening method based on the established QSAR model can screen aptamers formed by site-directed mutation of bases. The results of intermolecular interactions and molecular docking displayed that the new aptamer was mainly bound to ricin A-chain, and its combination of ricin with ricin B-chain aptamer has a significant synergistic effect. Due to the aptamer with excellent performance and the good linear relationship between the relative resistance change and the vertical deformation of the micro-cantilever, the piezoresistive micro-cantilever aptasensor emerged a linear detection range from 7.5 pg mL-1 to 75 ng mL-1 (R2 = 0.949) with the limit of detection 750 fg mL-1 (S/N ≥ 3) for ricin. Furthermore, the proposed piezoresistive micro-cantilever sensor displayed excellent analytical performance with good reproducibility and high specificity.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Ricina , Técnica de Seleção de Aptâmeros/métodos , Aptâmeros de Nucleotídeos/química , Relação Quantitativa Estrutura-Atividade , Simulação de Acoplamento Molecular , Reprodutibilidade dos Testes , Técnicas Biossensoriais/métodos
5.
Sci Total Environ ; 857(Pt 1): 159311, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36216047

RESUMO

Composting is an effective alternative for recycling faecal sludge into organic fertilisers. A microflora-earthworm (Eisenia fetida) synergistic cooperation system was constructed to enhance the composting efficiency of faecal sludge. The impact of earthworms and carbon-rich wastes (rice straw (RS) and sawdust (S)) on compost properties, greenhouse gas emissions, and key microbial species of composting were evaluated. The addition of RS or S promoted earthworm growth and reproduction. The earthworm-based system reduced the volatile solid of the final substrate by 13.19-16.24 % and faecal Escherichia coli concentrations by 1.89-3.66 log10 cfu/g dry mass compared with the earthworm-free system. The earthworm-based system increased electrical conductivity by 0.322-1.402 mS/cm and reduced C/N by 56.16-64.73 %. The NH4+:NO3- ratio of the final faecal sludge and carbon-rich waste was <0.16. The seed germination index was higher than 80 %. These results indicate that earthworms contribute to faecal sludge maturation. Earthworm addition reduced CO2 production. The simultaneous addition of earthworms and RS system (FRS2) resulted in the lowest global warming potential (GWP). The microbial diversity increased significantly over time in the RS-only system, whereas it initially increased and later decreased in the FRS2 system. Cluster analysis revealed that earthworms had a more significant impact on the microbial community than the addition of carbon-rich waste. Co-occurrence networks for earthworm-based systems were simple than those for earthworm-free systems, but the major bacterial genera were more complicated. Highly abundant key species (norank_f_Chitinophagaceae and norank_f_Gemmatimonadaceae) are closely related. Microbes may be more cooperative than competitive, facilitating the conversion of carbon and nitrogen in earthworm-based systems. This work has demonstrated that using earthworms is an effective approach for promoting the efficiency of faecal sludge composting and reducing GWP.


Assuntos
Compostagem , Microbiota , Oligoquetos , Oryza , Animais , Esgotos/química , Carbono/análise , Aquecimento Global , Solo/química
6.
Bioact Mater ; 20: 663-676, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35891799

RESUMO

Cancer therapies based on energy conversion, such as photothermal therapy (PTT, light-to-thermal energy conversion) and photodynamic therapy (PDT, light-to-chemical energy conversion) have attracted extensive attention in preclinical research. However, the PTT-related hyperthermia damage to surrounding tissues and shallow penetration of PDT-applied light prevent further advanced clinical practices. Here, we developed a thermoelectric therapy (TET) based on thermoelectric materials constructed p-n heterojunction (SrTiO3/Cu2Se nanoplates) on the principle of light-thermal-electricity-chemical energy conversion. Upon irradiation and natural cooling-induced the temperature gradient (35-45 oC), a self-build-in electric field was constructed and thereby facilitated charges separation in bulk SrTiO3 and Cu2Se. Importantly, the contact between SrTiO3 (n type) and Cu2Se (p type) constructed another interfacial electric field, further guiding the separated charges to re-locate onto the surfaces of SrTiO3 and Cu2Se. The formation of two electric fields minimized probability of charges recombination. Of note, high-performance superoxide radicals and hydroxyl radicals' generation from O2 and H2O under catalyzation by separated electrons and holes, led to intracellular ROS burst and cancer cells apoptosis without apparent damage to surrounding tissues. Construction of bulk and interfacial electric fields in heterojunction for improving charges separation and transfer is also expected to provide a robust strategy for diverse applications.

7.
Small ; 18(12): e2106196, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35322558

RESUMO

Cell mechanical forces play fundamental roles in regulating cellular responses to environmental stimulations. The shortcomings of conventional methods, including force resolution and cellular throughput, make them less accessible to mechanical heterogeneity at the single-cell level. Here, a DNA tensioner platform is introduced with high throughput (>10 000 cells per chip) and pN-level resolution. A microfluidic-based cell array is trapped on "hairpin-structured" DNA tensioners that enable transformation of the mechanical information of living cells into fluorescence signals. By using the platform, one can identify enhanced mechanical forces of drug-resistant cells as compared to their drug-sensitive counterparts, and mechanical differences between metastatic tumor cells in pleural effusion and nonmetastatic histiocytes. Further genetic analysis traces two genes, VEGFA and MINK1, that may play deterministic roles in regulating mechanical heterogeneities. In view of the ubiquity of cells' mechanical forces in the extracellular microenvironment (ECM), this platform shows wide potential to establish links of cellular mechanical heterogeneity to genetic heterogeneity.


Assuntos
DNA , Microfluídica
8.
J Neurosci Res ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36345215

RESUMO

Seizures are a frequent symptom of unruptured brain arteriovenous malformations (bAVMs). However, the brain regions responsible for these seizures remain unclear. To identify the brain regions causally involved in bAVM-related seizures, we retrospectively reviewed 220 patients with unruptured bAVMs. Using voxel-based lesion-symptom mapping (VLSM) analyses, we tested whether individual brain regions were associated with unruptured bAVM-related seizures. The result revealed that unruptured bAVMs causing seizures are anatomically heterogeneous at the voxel level. Subsequently, lesion network mapping (LNM) analyses was performed to determine whether bAVMs causing seizures belonged to a distributed brain network. LNM analyses indicated that these lesions were located in a functional network characterized by connectivity to the left caudate and precuneus. Moreover, the discrimination performance of the identified seizure network was evaluated in discovery set by calculating the individualized network damage score and was tested in validation set. Based on the calculated network damage scores, patients were divided into low-, medium-, and high-risk groups. The prevalence of seizures significantly differed among the three risk categories in both discovery (p = .003) and validation set (p = .004). Finally, we calculated the percentage of voxels in the canonical resting-state networks that overlapped with the seizure-susceptible brain regions to investigate the involvement of resting-state networks. With an involvement percentage over 50%, the frontoparietal control (82.9%), limbic function (76.7%), and default mode network (69.3%) were considered to be impacted in bAVM-related seizures. Our study identified the seizure-susceptible brain regions for unruptured bAVMs, which could be a plausible neuroimaging biomarker in predicting possible seizures.

9.
IEEE Trans Cybern ; PP2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36355726

RESUMO

For the centralized optimization, it is well known that adding one momentum term (also called the heavy-ball method) can obtain a faster convergence rate than the gradient method. However, for the distributed counterpart, there is quite few results about the effect of added momentum terms on the convergence rate. This article is aimed at studying the issue in the distributed setup, where N agents minimize the sum of their individual cost functions using local communication over a network. The cost functions are twice continuously differentiable. We first study the algorithm with one momentum term and develop a distributed heavy-ball (D-HB) method by adding one momentum term on to the distributed gradient algorithm. By borrowing tools from the control theory, we provide a simple convergence proof and an explicit expression of the optimal convergence rate. Furthermore, we consider adding two momentum terms case and propose a distributed double-heavy-ball (D-DHB) method. We show that adding one momentum term allows faster convergence while adding two momentum terms does not perform any superiorities. Finally, simulation examples are given to illustrate our findings.

10.
Front Neurosci ; 16: 1045585, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425476

RESUMO

Introduction: Pseudocontinuous Arterial Spin Labeling (pCASL) perfusion imaging allows non-invasive quantification of regional cerebral blood flow (CBF) as part of a multimodal magnetic resonance imaging (MRI) protocol. This study aimed to compare regional CBF in autism spectrum disorders (ASD) individuals with their age-matched typically developing (TD) children using pCASL perfusion imaging. Materials and methods: This cross-sectional study enrolled 17 individuals with ASD and 13 TD children. All participants underwent pCASL examination on a 3.0 T MRI scanner. Children in two groups were assessed for clinical characteristics and developmental profiles using Autism Behavior Checklist (ABC) and Gesell development diagnosis scale (GDDS), respectively. We compared CBF in different cerebral regions of ASD and TD children. We also assessed the association between CBF and clinical characteristics/developmental profile. Results: Compared with TD children, individuals with ASD demonstrated a reduction in CBF in the left frontal lobe, the bilateral parietal lobes, and the bilateral temporal lobes. Within the ASD group, CBF was significantly higher in the right parietal lobe than in the left side. Correlation analysis of behavior characteristics and CBF in different regions showed a positive correlation between body and object domain scores on the ABC and CBF of the bilateral occipital lobes, and separately, between language domain scores and CBF of the left frontal lobe. The score of the social and self-help domain was negatively correlated with the CBF of the left frontal lobe, the left parietal lobe, and the left temporal lobe. Conclusion: Cerebral blood flow was found to be negatively correlated with scores in the social and self-help domain, and positively correlated with those in the body and object domain, indicating that CBF values are a potential MRI-based biomarker of disease severity in ASD patients. The findings may provide novel insight into the pathophysiological mechanisms of ASD.

11.
Dev Comp Immunol ; : 104595, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36427557

RESUMO

Toll-interacting protein (Tollip) is an important negative regulator of Toll-like receptor-mediated innate immunity by preventing excessive proinflammatory responses. The structure and function of Tollip have been well identified in mammals, but the piscine Tollip remains poorly understood. In the present study, a homologue of Tollip was identified and characterized from blunt snout bream (named MaTollip), which was composed of an 831 bp open reading frame encoding a protein of 276 amino acids. Phylogenetic analysis indicated that MaTollip is a novel member of Tollip family and possessed the highest similarity to that of grass carp (99.28%). Multiple alignment of amino acid sequence showed that MaTOLLIP shared a high degree of structural conservation, including a TBD domain, a C2 domain and a CUE domain, with its counterparts from other vertebrates. With regard to tissue-specific expression without immune challenge, MaTollip was constitutively expressed in a wide range of normal tissues, with the highest in the head-kidney and the lowest in the intestine. MaTollip expression in the head-kidney was strongly upregulated upon LPS stimulation and A. hydrophila infection. Fluorescence microscopic analysis revealed that the green fluorescent protein-TOLLIP was localized predominantly in the cytoplasm of EPC cells in a dot-like state. When MaTollip was overexpressed in HEK-293T and EPC cells, it could significantly inhibit the activity of nuclear factor-κB (NF-κB) promoter in a dose dependent manner. MaTollip overexpression in MAF cells lowered drastically the transcriptional expression level of lipopolysaccharide-induced proinflammatory cytokines (IL-1ß, IL-6 and IL-8), whereas they were dramatically promoted by MaTollip knock down with siRNA. Taken together, this study demonstrated that MaTollip played a pivotal role in mediating host innate immune response to pathogen invasion, and unveiled the involvement of MaTollip in NF-κB-mediated transcription of inflammation genes, which paved the way for further studies of immune negative regulation mechanisms mediated by Tollip in fish.

12.
Cell Stem Cell ; 29(11): 1562-1579.e7, 2022 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-36332570

RESUMO

During fetal development, human hematopoietic stem cells (HSCs) colonize the bone marrow (BM), where they self-renew and sustain hematopoiesis throughout life; however, the precise timepoint at which HSCs seed the BM is unclear. We used single-cell RNA-sequencing to map the transcriptomic landscape of human fetal BM and spleen hematopoietic stem/progenitor cells (HSPCs) and their microenvironment from 10 to 14 post-conception weeks (PCWs). We further demonstrated that functional HSCs capable of reconstituting long-term multi-lineage hematopoiesis in adult NOG mice do not emerge in the BM until 12 PCWs. In contrast, functional HSCs were not detected in the spleen by 14 PCWs. By comparing the niche-HSPC interactions between BM and spleen, we identified ligand-receptor pairs likely to be involved in fetal HSC migration and maintenance. Our work paves the way for research into the mechanisms underlying HSC colonization in human fetal BM and provides invaluable resources for future studies on HSC development.


Assuntos
Medula Óssea , Células-Tronco Hematopoéticas , Adulto , Humanos , Camundongos , Animais , Hematopoese/genética , Células da Medula Óssea , Análise de Sequência de RNA
13.
J Colloid Interface Sci ; 632(Pt A): 44-53, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36403376

RESUMO

Nickel-iron oxides are competitive electrocatalysts for oxygen evolution reaction, but their practical applications are restricted by the less-than-desirable intrinsic activity and working stability. To tackle the challenge, surface coordination chemistry is applied to the nickel-iron oxides through a complex-assisted in-situ crystal growth strategy. The ethylenediaminetetraacetate (EDTA) coordinated NixFe3-xO4 (NixFe3-xO4-EDTA) is prepared by a simple one-pot hydrothermal process. The coordinated EDTA molecules can deeply alter the surface coordination structure of the NixFe3-xO4. The NixFe3-xO4-EDTA demonstrates outstanding intrinsic activity towards oxygen evolution reaction, requiring only a small overpotential of 180 mV to reach 10 mA cm-2 in 1.0 M KOH. Moreover, the NixFe3-xO4-EDTA exhibits extremely stable long-term working stability. Density functional theory calculations show that the highly enhanced intrinsic activity is attributed to the surface coordinated EDTA-induced favorable electronic structure and coordination environment, which tunes the adsorption strength of the intermediates and optimizes the energetics of the elementary steps, while the high stability is ascribed to the strong coordination ability of EDTA.

14.
Nat Prod Res ; : 1-6, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377715

RESUMO

Momordica charantia L. (M. charantia) is an annual climbing herb in Cucurbitaceae. It is not only a food, but also a drug with a long history of application. This study aims to isolate and identify the chemical constituents form M. charantia and evaluate their inhibiting effect on Hcy-induced proliferation of VSMCs. Silica column chromatography, ODS silica column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC were used to obtain one new compound (1). The inhibition on Hcy-induced proliferation of VSMCs 1 was tested through MTT method. As a result, 1 could partially rescue Hcy-induced proliferation of VSMCs at both 5 and 25 µM.

15.
Int J Mol Sci ; 23(21)2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36361568

RESUMO

Lymph node metastasis is associated with poor prognosis of oral squamous cell carcinoma (OSCC), and few studies have explored the relevance of postoperative lymphatic drainage (PLD) in metastatic OSCC. Alpha-enolase (ENO1) is a metabolic enzyme, which is related to lymphatic metastasis of OSCC. However, the role of ENO1 in PLD in metastatic OSCC has not been elucidated. Herein, we collected lymphatic drainage after lymphadenectomy between metastatic and non-metastatic lymph nodes in OSCC patients to investigate the relationship between ENO1 expression and metastasis, and to identify the proteins which interacted with ENO1 in PLD of patients with metastatic OSCC by MS/GST pulldown assay. Results revealed that the metabolic protein apolipoprotein C-III (ApoC3) was a novel partner of ENO1. The ENO1 bound to ApoC3 in OSCC cells and elicited the production of interleukin (IL)-8, as demonstrated through a cytokine antibody assay. We also studied the function of IL-8 on Jurkat T cells co-cultured with OSCC cells in vitro. Western blot analysis was applied to quantitate STAT3 (signal transducer and activator of transcription 3) and p-STAT3 levels. Mechanistically, OSCC cells activated the STAT3 signaling pathway on Jurkat T cells through IL-8 secretion, promoted apoptosis, and inhibited the proliferation of Jurkat T cells. Collectively, these findings illuminate the molecular mechanisms underlying the function of ENO1 in metastasis OSCC and provide new strategies for targeting ENO1 for OSCC treatment.


Assuntos
Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Bucais , Humanos , Neoplasias Bucais/patologia , Carcinoma de Células Escamosas/patologia , Fator de Transcrição STAT3/metabolismo , Apolipoproteína C-III , Interleucina-8/metabolismo , Carcinoma de Células Escamosas de Cabeça e Pescoço , Linhagem Celular Tumoral , Regulação Neoplásica da Expressão Gênica , Metástase Linfática , Fosfopiruvato Hidratase/metabolismo , Proliferação de Células , Proteínas de Ligação a DNA/metabolismo , Biomarcadores Tumorais
16.
Int J Mol Sci ; 23(21)2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36362196

RESUMO

Macrophage-expressed gene 1 proteins (Mpeg1/Perforin-2 (PRF2)) are a family of pore-forming proteins (PFPs) which can form pores and destroy the cell membrane of invading pathogens. However, little information is available regarding the function of Mpeg1 in the giant triton snail Charonia tritonis. In this study, a homolog of Mpeg1 (Ct-Mpeg1) was identified in C. tritonis. The predicted protein of Ct-Mpeg1 contains several structural features known in Mpegs, including a membrane attack complex/perforin (MACPF) domain and single transmembrane region. The Ct-Mpeg1 gene was constitutively expressed in almost all tissues examined except in the proboscis, with the highest expression level observed in the mantle. As a typical pore-forming protein, Ct-Mpeg1 has antibacterial activities against Vibrio (including Vibrio alginolyticus and Vibrio parahaemolyticus). In addition, rCt-Mpeg1 challenge to V. alginolyticus represses the expression of most outer membrane protein synthesis-related genes and genes involved in the TCA cycle pathway, which will lead to reduced outer membrane protein synthesis and less energy capacity. This is the first report to characterize the macrophage-expressed gene 1 protein in C. tritonis, and these results suggest that macrophage-expressed gene 1 protein Ct-Mpeg1 is an important immune molecule of C. tritonis that is involved in the bacterial infection resistance of Vibrio, and this study may provide crucial basic data for the understanding of the innate immunity system of C. tritonis.


Assuntos
Anti-Infecciosos , Proteínas de Membrana , Animais , Perforina/metabolismo , Proteínas de Membrana/metabolismo , Caramujos/genética , Imunidade Inata/genética , Macrófagos/metabolismo , Anti-Infecciosos/metabolismo
17.
ACS Appl Mater Interfaces ; 14(45): 51307-51317, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36320188

RESUMO

Passive daytime radiative cooling (PDRC) technology provides an eco-friendly cooling strategy by reflecting sunlight reaching the surface and radiating heat underneath to the outer space through the atmospheric transparency window. However, PDRC materials face challenges in cooling performance degradation caused by outdoor contamination and requirements of easy fabrication approaches for scale-up and high cooling efficiency. Herein, a polymer composite coating of polystyrene, polydimethylsiloxane and poly(ethyl cyanoacrylate) (PS/PDMS/PECA) with superhydrophobicity and radiative cooling performance was fabricated and demonstrated to have sustained radiative cooling capability, utilizing the superhydrophobic self-cleaning property to maintain the optical properties of the coating surface. The prepared coating is hierarchically porous which exhibits an average solar reflectance of 96% with an average emissivity of 95% and superhydrophobicity with a contact angle of 160°. The coating realized a subambient radiative cooling of 12.9 °C in sealed air and 7.5 °C in open air. The self-cleaning property of the PS/PDMS/PECA coating helped sustain the cooling capacity for long-term outdoor applications. Moreover, the coating exhibited chemical resistance, UV resistance, and mechanical durability, which has promising applications in wider fields.

18.
Biochem Biophys Res Commun ; 636(Pt 1): 1-9, 2022 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-36332469

RESUMO

Dyslipidemia and inflammation have great roles in the development of diabetic nephropathy (DN). Oleanolic acid (OA) is a natural triterpenoid that possesses multiple pharmacological properties including anti-oxidation, anti-inflammatory and hypoglycemia. In the present study, the effects of OA on diabetic kidney disease (DKD) and its underlying mechanisms were investigated in DKD rats. Twenty-five of a total thirty-five male Sprague-Dawley (SD) rats were used to establish for Type 2 diabetes mellitus (T2DM) model by high-fat diet combined with streptozotocin (STZ). Then rats were randomly assigned into four group: control group (n = 10), T2DM group (n = 9), OA (50 mg/kg) group (n = 7), OA (100 mg/kg) group (n = 8). Rats were sacrificed at the end of 18 weeks after feeding by intraperitoneal injection of pentobarbital sodium. Body weight (BW), fasting blood glucose (FBG), kidney weight (KW), serum lipid, 24-h urinary microalbumin (UMA), serum creatinine (Scr) and uric acid (UA) were measured. Histopathological changes were observed by PAS staining and electron microscope. The expressions of nephrin, CD68, Collagen-IV, AMPK, p-AMPK, PGC-1α, TLR4, NF-κB and TGF-ß1 in kidney were also detected by immunohistochemistry or western blot. OA significantly decreased the levels of FBG, kidney index (KI), serum lipid levels, 24 h UMA, Scr, UA in diabetic rats. Additionally, OA obviously attenuated renal lipid accumulation and renal structure abnormalities in diabetic rats. Furthermore, the expression levels of nephrin, p-AMPK/AMPK, PGC-1α were elevated, while CD68, Collagen-IV, TLR4, NF-κB and TGF-ß1 expressions were decreased in renal tissues of OA treated diabetic rats. OA showed dose-independent. OA can alleviate renal injury in diabetic rats through improving lipid metabolism and inflammation via AMPK/PGC-1α and TLR4/NF-κB signaling pathway.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Ácido Oleanólico , Animais , Masculino , Ratos , Proteínas Quinases Ativadas por AMP/metabolismo , Colágeno/metabolismo , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Nefropatias Diabéticas/tratamento farmacológico , Nefropatias Diabéticas/prevenção & controle , Nefropatias Diabéticas/metabolismo , Inflamação/patologia , Rim/metabolismo , Lipídeos/sangue , NF-kappa B/metabolismo , Ácido Oleanólico/farmacologia , Ratos Sprague-Dawley , Receptor 4 Toll-Like/metabolismo , Fator de Crescimento Transformador beta1/metabolismo
19.
Funct Plant Biol ; 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36396124

RESUMO

Plant diseases seriously damage crop production, and most plant diseases are caused by fungi. Fungal cell walls contain chitin, a highly conserved component that is widely recognised by plants as a PAMP (pathogen-associated molecular pattern) to induce defence responses. The molecular mechanisms that function downstream of chitin-triggered intracellular phosphorylation remain largely unknown. In this study, we performed quantitative phosphoproteomics analysis to study protein phosphorylation changes in the plasma membrane after chitin treatment in Arabidopsis thaliana L. seedlings. Proteins with altered phosphorylation status after chitin treatment participated in biological processes ranging from signalling, localisation, and transport, to biogenesis, processing, and metabolism, suggesting that PAMP signalling targets multiple processes to coordinate the immune response. These results provide important insights into the molecular mechanism of chitin-induced plant immunity.

20.
Tissue Cell ; 80: 101984, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36434828

RESUMO

Determining myocardial infarction (MI) and mechanical asphyxia (MA) was one of the most challenging tasks in forensic practice. The present study aimed to investigate the potential of fatty acid (FAs) metabolism, and lipid alterations in determining MI and MA. MA and MI mouse models were constructed, and metabolic profiles were obtained by LC-MS-based untargeted metabolomics. The metabolic alterations were explored using the PCA, OPLS-DA, the Wilcoxon test, and fold change analysis. The contents of lipid droplets (LDs) were detected by the transmission scanning electron microscope and Oil red O staining. The immunohistochemical assay was performed to detect CD36 and dysferlin. The ceramide was assessed by LC-MS. PCA showed considerable differences in the metabolite profiles, and the well-fitting OPLS-DA model was developed to screen differential metabolites. Thereinto, 9 metabolites in the MA were reduced, while metabolites were up- and down-regulated in MI. The increased CD36 suggested that MI and MA could enhance the intake of FAs and disturb energy metabolism. The increased LDs, decreased dysferlin, and increased ceramide (C18:0, C22:0, and C24:0) were observed in MI groups, confirming the lipid deposition. The present study indicated significant differences in myocardial FAs metabolism and lipid alterations between MI and MA, suggesting that FAs metabolism and related proteins, certain ceramide may harbor the potential as biomarkers for discrimination of MI and MA.

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