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1.
Chemosphere ; 239: 124759, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31518920

RESUMO

Ammonia is an important environmental stress factor in aquaculture. Long-term ammonia stress could affect the normal growth, and also increase the risk for the occurrence of various diseases. In order to learn the mechanism that ammonia caused the outbreak of the shrimp disease, transcriptomics and metabolomics approaches were used to analyze the differential expressions of the genes in hemocytes and different metabolites in the serum of the Pacific white shrimp Litopenaeus vannamei under ammonia exposure. Transcriptional analysis showed that 17 cell apoptosis related genes, seven phagocytosis related genes, 10 immunity related genes and seven cell cycle and lipid metabolism related genes showed differential expressions after ammonia exposure. Metabolomics analysis on the serum showed that 25 differential metabolites were identified in positive and negative ion patterns. They are involved in purine metabolism, amino acids metabolism and lipid metabolism. Injection of two up-regulated metabolites triethanolamine and oxypurinol to normal shrimp could induce apoptosis in normal shrimp. The total hemocytes counts in shrimp showed a significant decrease and the apoptotic cell ratio increased significantly under ammonia exposure. These results suggested that ammonia exposure increased the apoptosis of hemocytes, which affected the immunity of shrimp, and thus caused susceptibility to pathogenic infection. These data will help us understand the mechanism of ammonia stress leading to the immunity decline of shrimp.

2.
Talanta ; 206: 120199, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514856

RESUMO

A new class of intracellular signal amplification approach, integrating biodegradable manganese dioxide (MnO2) nanosheet with target-triggered DNAzyme recycling amplification in one nanosystem, was developed for highly specific and sensitive monitoring of microRNAs (miRNAs) in living cells. Briefly, the MnO2 nanosheets were employed as carrier and quencher for the hairpin-locked-DNAzyme strands (H1). Upon entering cells, the surface-adsorbed strands (H1) can be released due to the degradation of the MnO2 nanosheets by cellular glutathione. Subsequently, the hybridization reaction between target miRNAs and H1 probe induced the conformation alteration of the hairpin probes H1, formed an "active" DNAzyme. With the assistor of Mg2+, the DNAzyme was activated and induced the release of the fluorophores labeled DNA fragment, which achieved the restoration of fluorescence signal. Meanwhile, the target molecules was released and hybridized with the other H1 strand to initiate another cycle of activation, cleavage, and turnovers, which producing enhanced fluorescence signal for sensitive analysis of intracellular miRNAs. Furthermore, fluorescence imaging experiments demonstrated that the MnO2-DNAzyme nanosystem could visually detect microRNA-21 in cancer cells. The proposed strategy provides a good platform for highly sensitive detection and imaging analysis of various intracellular miRNAs in situ.

3.
J Hazard Mater ; 382: 121120, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31487667

RESUMO

Low-cost and high-performance materials or techniques that could synergistically remove inorganic heavy metals and organic pollutants in a simple manner are highly desired. Herein, we report a simple and facile strategy by converting poisonous heavy metals into photocatalyst for the in-situ photodegradation of organic pollutants employing steel slag-derived calcium silicate hydrate (CSH). The CSH was synthesized by alkali activation method and showed hierarchical structure and amorphous phase. And, the material exhibited excellent adsorption performance towards all selected heavy metals. After adsorption, the heavy metals were converted into the corresponding amorphous metal hydroxides on the surface of CSH. The resulting CSH-supported amorphous metal hydroxides can act as visible-light photocatalysts for the photodegradation of organic pollutants. The optimal results for the whole water purification route using CSH are > 100 mg/g adsorption capacity for Cu2+ and ˜63% / 8 h photodegradation efficiency for methylene blue under visible light. The total cost for the whole route is < 0.1 $/g pollutants, much lower than traditional technologies. The strategy using steel slag derived-CSH not only meets the requirements for high-performance and low-cost materials, but also resolves the challenging issues of developing an all-in-one treatment for heavy metal ions and organic pollutants, which will be of great significance to wastewater purification.

4.
Acta Neurochir Suppl ; 127: 69-75, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31407066

RESUMO

BACKGROUND: Subarachnoid hemorrhage (SAH) is a severe and emergent cerebrovascular disease, the prognosis of which usually very poor. Microthrombi formation highlighted with inflammation occurs early after SAH. As the main cause of DCI, microthrombosis associated with the prognosis of SAH. The aim of this study was to show HSP90 inhibitor 17-AAG effect on microthrombosis after SAH in rats. METHODS: Ninety-five SD rats were used for the experiment. For time course study, the rats were randomly divided into five groups: sham group and SAH group with different time point (1d, 2d, 3d, 5d). Endovascular perforation method was conducted for SAH model. Neurological score, SAH grade, and mortality were measured after SAH. The samples of the left hemisphere brain were collected. The expression of HSP90 was detected by Western blot. The microthrombosis after SAH in rats' brain was detected by immunohistochemistry. For mechanism study, rats were randomly divided into three groups: sham, SAH + vehicle, and SAH +17-AAG (n = 6/group). 17-AAG was given by intraperitoneal injection (80 mg/kg) 1 h after SAH. Neurological function were measured at 24 h after SAH. The expression of RIP3, NLRP3, ASC, and IL-1ß was measured by Western blot. Microthrombosis was detected by immunohistochemistry. RESULTS: Our results showed that the HSP90 protein level increased and peaked at 2 days after SAH. Microthrombosis caused by SAH was increased in 1 day and peaked at 2 days after SAH. Administration HSP90 specific inhibitor 17-AAG reduced expression of RIP3, NLRP3, ASC, and IL-1ß, reduced microthrombosis after SAH, and improved neurobehavior when compared to vehicle group. CONCLUSIONS: 17-AAG can ameliorate microthrombosis via HSP90/RIP3/NLRP3 pathway and improve neurobehavior after SAH.


Assuntos
Inibidores Enzimáticos , Proteína 3 que Contém Domínio de Pirina da Família NLR , Hemorragia Subaracnóidea , Trombose , Animais , Córtex Cerebral , Inibidores Enzimáticos/farmacologia , Proteínas de Choque Térmico HSP90 , Inflamação , Ratos , Ratos Sprague-Dawley , Proteína Serina-Treonina Quinases de Interação com Receptores , Hemorragia Subaracnóidea/tratamento farmacológico , Hemorragia Subaracnóidea/metabolismo , Trombose/tratamento farmacológico
5.
Biosens Bioelectron ; 148: 111832, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31706173

RESUMO

Fluorescence-activated cell sorting (FACS) has rarely been applied to screening of microorganisms because of poor detection resolution, which is compromised by poor stability, toxicity, or interference from background fluorescence of the fluorescence sensors used. Here, a fluorescence-based rapid high-throughput cell sorting method was first developed using a fluorescence resonance energy transfer (FRET) fluorescent nanoprobe NP-RA, which was constructed by coating a silica nanoparticle with Rhodamine B and methyl-red (an azo dye). Rhodamine B (inner layer) is the FRET donor and methyl-red (outer layer) is the acceptor. This ready-to-use NP-RA is non-fluorescent, but fluoresces once the outer layer is degraded by microorganisms. In our experiment, NP-RA was ultrasensitive to model strain Shewanella decolorationis S12, showing a broad detection range from 8.0 cfu/mL to 8.7 × 108 cfu/mL under confocal laser scanning microscopy, and from 1.1 × 107 to 9.36 × 108 cfu/mL under a fluorometer. In addition, NP-RA bioimaging can clearly identify other azo-respiring cells in the microbial community, including Bosea thiooxidans DSM 9653 and Lysinibacillus pakistanensis NCCP-54. Furthermore, the fluorescent probe NP-RA is compatible with downstream FACS so that azo-respiring cells can be rapidly sorted out directly from an artificial microbial community. To our knowledge, no fluorescent nanoprobe has yet been designed for tracking and sorting azo-respiration functional microorganisms.

6.
J Cell Physiol ; 235(2): 1001-1012, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31240707

RESUMO

Emerging evidence has indicated that long noncoding RNA (lncRNAs) play crucial roles in regulating thyroid cancer (TC) development. Linc00210 is a newly identified lncRNA which plays an oncogenic role in hepatocellular carcinoma and nasopharyngeal carcinoma, but whether Linc00210 can modulate the development of TC remains elusive. Here, we found that Linc00210 expression was upregulated in TC tissues compared to the matched noncancerous tissues. Overexpression of Linc00210 augmented the proliferation, migration, and invasion of TC cells. Mechanistically, Linc00210 served as a sponge for miR-195-5p, thereby counteracting its ability in downregulating the expression of IGF1R and the activation of PI3K/Akt signaling. Moreover, inhibition of Linc00210 suppressed the growth of TC cells in nude mice. Our findings for the first time uncovered the oncogenic property of Linc00210 in TC.

7.
Anal Biochem ; 588: 113468, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31585097

RESUMO

Interleukin 6 (IL-6) is an interleukin that acts as both a proinflammatory and anti-inflammatory cytokine. It can be used as a potential diagnostic biomarker for sepsis. The aim of this study was to establish an easy-to-use detection kit for rapid, quantitative and on-site detection of IL-6. To develop the new IL-6 quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on europium nanoparticles (Eu-np) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of parallel analysis, linearity, sensitivity, precision, accuracy, specificity and clinical sample analysis. Two-hundred and fourteen serum samples were used to carry out the clinical sample analysis. The new IL-6 quantitative detecting kit exhibited a wide linear range (2-500 pg/mL) and a good sensitivity (0.37 pg/mL). The intra-assay coefficient of variation (CV) and the inter-assay CV were 5.92%-8.87% and 7.59%-9.04%, respectively. The recovery rates ranged from 102% to 106%. Furthermore, a high correlation (n = 214, r = 0.9756, p < 0.01) was obtained when compared with SIEMENS CLIA IL-6 kit. Thus, the new quantitative method for detecting IL-6 has been successfully established. The results indicated that the newly-developed strip based on Eu-np combined with LFIA was a facile, fast, highly sensitive, low-cost, reliable biosensor and suitable for rapid and point-of-care test (POCT) for IL-6 in serum.

8.
Int J Cancer ; 146(2): 475-486, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31107971

RESUMO

Long noncoding RNAs (lncRNAs) promote cell proliferation, migration, invasion and castration resistance in prostate cancer (PCa). Understanding the inherited molecular mechanisms by which lncRNAs contribute to the progression of PCa to a lethal disease could have an important impact on cancer detection, diagnosis and prognosis. In our study, PCa-associated lncRNA transcripts from RNA-seq data were identified and screened via bioinformatics analysis, NCBI annotations and literature review. We identified a novel lncRNA, lncAPP (lncRNA activated in PCa progression), which activates in PCa progression and is expressed in primary tumor tissues and urine samples of patients with localized or advanced PCa. Urinary-based lncAPP is a promising biomarker for predicting PCa progression. In vitro and in vivo studies demonstrated that lncAPP enhanced cell proliferation and promoted migration and invasion. The underlying mechanism of lncRNA was investigated by RNA immunoprecipitation, dual-luciferase reporter system assay, etc. Upregulation of lncAPP promoted cell migration and invasion via competitively binding miR218 to facilitate ZEB2/CDH2 expression. In addition, in vivo subcutaneous tumor xenograft models and tail intravenously injection metastatic models were constructed to evaluate lncRNA function. Targeting lncAPP/miR218 axis in cell lines and tumor xenografts restrained tumor progression properties both in vitro and in vivo. These results establish that lncAPP/miR218 axis plays a critical role in PCa progression, and they also suggest new strategies to prevent tumor progression for therapeutic purposes.

9.
Biomed Pharmacother ; 121: 109623, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31731187

RESUMO

BACKGROUND: Oral squamous cell carcinoma (OSCC) is a type of oral malignancy. Long non-coding RNAs (lncRNAs) have been shown to be related to the occurrence and development of many cancers. Here, we aimed to study the role and molecular mechanism of lncRNA Homeobox A11 antisense RNA (HOXA11-AS) in OSCC. METHODS: The expression levels of HOXA11-AS, miR-98-5p and Y box binding protein 2 (YBX2) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8), flow cytometry and transwell assays were utilized to determine the proliferation, apoptosis, migration and invasion of OSCC cells. Western blot (WB) analysis was conducted to measure the levels of apoptosis, epithelial-mesenchymal transition (EMT), proliferation-related proteins and YBX2 protein. Besides, Dual-luciferase reporter, RNA immunoprecipitation (RIP) and RNA pull down assays were carried out to examine the relationship among HOXA11-AS, miR-98-5p and YBX2. The mice xenograft models were constructed to further determine the effect of HOXA11-AS on OSCC tumor growth in vivo. RESULTS: HOXA11-AS was highly expressed in OSCC tissues and cells. Knockdown of HOXA11-AS significantly reduced proliferation, migration, invasion and EMT, while promoted apoptosis of OSCC cells. MiR-98-5p was a target of HOXA11-AS, and its inhibitor could revert the inhibition effect of silenced-HOXA11-AS on the progression of OSCC. Also, YBX2 was a target of miR-98-5p, and its overexpression could invert the suppression effect of miR-98-5p overexpression on the progression of OSCC. YBX2 expression was regulated by HOXA11-AS and miR-98-5p. Furthermore, HOXA11-AS silencing could reduce the tumor growth of OSCC in vivo. CONCLUSION: HOXA11-AS plays an active role in the progression of OSCC, and the discovery of HOXA11-AS/miR-98-5p/YBX2 axis provides new therapeutic targets for OSCC.

10.
Carbohydr Polym ; 228: 115358, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635727

RESUMO

Following the main idea of a two-step method, microcrystalline cellulose is firstly pretreated to prepare regenerated cellulose (RC) with low crystallinity. Then, RC is continuously hydrolyzed to sugar in aqueous system by microwave driving. With the establishment of ZrO2 contained low acid catalytic system, an advanced route driven by microwave radiation to induce highly efficient hydrolysis of RC to sugar is formed. Due to the effect of ZrO2 on the molecular chain of cellulose, the recrystallization of RC is obviously weakened, and higher hydrolysis reactivity is achieved. Under the optimal conditions, the average conversion of RC and the yield of total reducing sugar highly reach 98.4 ±â€¯0.5% and 97.9 ±â€¯0.6%, respectively. The result is extremely superior to the efficiency of hydrolysis initiated only by pure acid. As a result, a novel and simple thinking to establish an advanced two-step methodology to hydrolyze cellulose to sugar with high efficiency is achieved.

11.
J Nanosci Nanotechnol ; 20(4): 2495-2502, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492267

RESUMO

A major reduction in energy consumption and the costs of catalysts will be required in future chemical manufacturing processes. To reach this goal, the transitional metal oxides (TMOs) as photocatalysts under solar energy have been widely studied. Nb2O5, as a promising photocatalyst, has attracted increasing attention owing to their unique properties. However, the intrinsic large bandgap of Nb2O5 hinder its potential applications in a variety of fields. Herein, we report an effective and simple strategy to synthesize black mesoporous Nb2O5-x nanorods (BMNb) with abundant oxygen vacancies. The formation of oxygen vacancy reduces the bandgap of Nb2O5 which extend the photoresponse from the ultraviolet to the visible and infrared light regions. In addition, The mesoporous structure of BMNb lead to a higher surface area than the as-prepared Nb2O5 precursor (36.24 m²/g cf 8.69 m²/g). Benefitting from coordinated regulation of structure and composition, the BMNb exhibits better photocatalytic performance than Nb2O5 in aerobic oxidative coupling of amines to imines under visible light irradiation at room temperature. The yield of BMNb for benzylamine oxidation increases by 63% over the Nb2O5. This work could open new perspectives to design TMOs with enhanced photocatalytic properties.

12.
Sci Total Environ ; : 135730, 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31791761

RESUMO

In southwestern China, karst river water is the main source of water for humans. As emerging pollutants, antibiotics have contaminated karst river water in some areas for a long time. Microbiota is highly susceptible to environmental changes, and can be used in tracing the source of antibiotics in complex systems such as karst water. Ten karst river water samples were collected along the river flow. The diversity and structure of the microbial community were analyzed together with environmental factors through correlation analysis, the random forest algorithm and co-occurrence network analysis. At genus level, Arcobacter was significantly positively correlated with the antibiotics, indicating that Arcobacter and antibiotics probably came from the same source. Based on co-occurrence network analysis between microbes, the microbial community was divided into eight modules, and the relative abundance of three modules was significantly correlated with antibiotics. The co-occurrence networks between bacteria and antibiotic resistance genes (ARGs) showed that pathogenic bacteria potentially carried multiple ARGs. This could increase the disease risk to humans and disease transmission in the study area. When river water flowed underground, the concentration of antibiotics decreased for the two underground river outlet sites, but abundance of bacteria and ARGs increased. Microbial source tracking studies showed that contamination was derived from humans rather than livestock. The ranking importance of prediction for antibiotics in this study area from random forest follows: specific bacteria Arcobacter > ARGs > ecological clusters. This study will be helpful in identifying the effect of antibiotics discharge on the microbial community, improving evaluation of antibiotics' risks and contaminants source tracking.

13.
J BUON ; 24(5): 1905-1912, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31786854

RESUMO

PURPOSE: Triple negative breast cancer (TNBC) refers to breast cancer that lacks progesterone receptor (PR), estrogen receptor (ER) and human epidermal growth factor receptor 2 (HER2). MicroRNA-365 (miR-365), a new-found microRNA, has been reported to possess significant functions in a multitude of human cancers. The purpose of this study was to detect thoroughly the molecular mechanisms of miR-365 that underlie the progress of TNBC. METHODS: The mRNA levels of miR-365 and A Disintegrin and Metalloprotease 10 (ADAM10) were measured by real-time polymerase chain reaction (RT-PCR). Luciferase activity report was applied to verify that ADAM10 was a direct target gene of miR-365. Cell proliferation ability was measured by MTT assay. Transwell assay was utilized to test cell migratory and invasive abilities. RESULT: We found that miR-365 was low-expressed in breast cancer tissues and 5 TNBC cell lines compared with the paracancerous samples and a normal cell line MCF10A. Meanwhile, we discovered that the expression of ADAM10 was higher in the 5 TNBC cell lines than in the normal cell line MCF10A. The proliferation, migration and invasion abilities were suppressed by overexpression of miR-365, whereas they were enhanced by interfering miR-365 in breast cancer. The luciferase reporter assay demonstrated that miR-365 directly targeted ADAM10 through directly binding to the 3'-untranslated region (3'-UTR). And the expression of ADAM10 was reduced by exogenous overexpression of miR-365, while it was increased by transfecting of miR-365 inhibitor in MDA-MB-231 and BT483 cells. Furthermore, re-expression of ADAM10 reversed partial functions of the suppressive roles on cell proliferation, migration and invasion by miR-365 TNBC. CONCLUSIONS: MiR-365 inhibited the proliferation, migration and invasion through directly binding to the 3'-UTR of ADAM10 mRNA in TNBC. It is suggested that miR-365/ADAM10 axis may present a new target for the treatment of breast cancer.

14.
Prog Mol Biol Transl Sci ; 168: 243-256, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31699318

RESUMO

Alzheimer's disease (AD) was first described by Alois Alzheimer in 1906 but the protein composition of neurofibrillary tangles and amyloid plaques was not decoded till about seven to eight decades later, respectively. The bulk of studies during the last four decades were focused on Aß amyloid and led to many human clinical trials, none of which showed any beneficial therapeutic effects. Though the outcome of prodromal Aß immunotherapy in carriers of AD-causing gene mutations is still awaited, this has led to a shift away from Aß-based, toward tau-based, therapeutic approaches. Currently, several Phase I and Phase II human clinical trials are underway, the majority of which involve active or passive tau immunization. It is quite possible that along with inhibition of tau pathology, use of neurotrophic compounds that can enhance neurogenesis and neuronal plasticity will be required to effectively prevent and restore cognitive deficits in AD and related conditions. Investments need to be made in drug development of such compounds.

15.
Environ Monit Assess ; 191(12): 739, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31709453

RESUMO

The provision capacity of ecosystem services is affected by land-use/land-cover (LULC) change and biophysical and socio-economic characteristics. Studies related to ecosystem service values (ESVs) and their impact factors can provide a good reference for natural capital management. In this paper, the temporal and spatial dynamics of LULC and ESVs in the Nansihu Lake basin, China, since the reform and opening of China were analyzed by a combined method of spatial analysis and correlation analysis, and the factors that influence ESV changes were discussed. The results indicated that the main LULC changes were the enormous loss of cultivated land and rapid urban land expansion since the policy of reformation and opening in 1978. The ESVs in the basin decreased by 1.64% (1.70 × 108 US$) from 1975 to 2015, and the main impact factors of this temporal change in ESVs were the changes in waterbodies and cultivated land. Spatial patterns of ESVs were significantly influenced by socio-economic factors in the basin, such as population density and intensity of land use. This research provides alternatives and robust information for policy-making processes for the Nansihu Lake basin, and similar work can be conducted in other parts of the country.

16.
Thromb Haemost ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705517

RESUMO

INTRODUCTION: Impaired plasminogen activation (PA) is causally related to the development of lung fibrosis. Prior studies demonstrate that enhanced PA in the lung limits the severity of scarring following injury and in vitro studies indicate that PA promotes matrix degradation and fibroblast apoptosis. These findings led us to hypothesize that increased PA in an in vivo model would enhance the resolution of established lung fibrosis in conjunction with increased myofibroblast apoptosis. METHODS: Transgenic C57BL/6 mice with doxycycline inducible lung-specific urokinase plasminogen activator (uPA) expression or littermate controls were treated (day 0) with bleomycin or saline. Doxycycline was initiated on days 1, 9, 14, or 21. Lung fibrosis, stiffness, apoptosis, epithelial barrier integrity, and inflammation were assessed. RESULTS: Protection from fibrosis with uPA upregulation from day 1 through day 28 was associated with reduced parenchymal stiffness as determined by atomic force microscopy. Initiation of uPA expression beginning in the late inflammatory or the early fibrotic phase reduced stiffness and fibrosis at day 28. Induction of uPA activity in mice with established fibrosis decreased lung collagen and lung stiffness while increasing myofibroblast apoptosis. Upregulation of uPA did not alter lung inflammation but was associated with improved epithelial cell homeostasis. CONCLUSION: Restoring intrapulmonary PA activity diminishes lung fibrogenesis and enhances the resolution of established lung fibrosis. This PA-mediated resolution is associated with increased myofibroblast apoptosis and improved epithelial cell homeostasis. These studies support the potential capacity of the lung to resolve existing scar in murine models.

18.
Clin Exp Med ; 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31734766

RESUMO

Coronary artery abnormalities (CAAs) are prominent during the acute Kawasaki disease (KD) episode and represent the major contributors to the long-term prognosis. Several meta-analysis and published scoring systems have identified hepatic dysfunction as an independent predictor of CAA risks. The medical records of 210 KD children were reviewed. Blood samples were collected from all subjects at 24 h pre-therapy and 48 h post-therapy, respectively. Liver function test (LFT) and inflammatory mediators were detected. Multivariate logistic regression analysis was conducted to identify the reliable biomarkers predicting whether CAAs existed or not in KD patients. 90.95% of KD patients had at least 1 abnormal LFT. Hypoalbuminemia was the most prevalent type of hepatic dysfunction, followed by elevated aspartate aminotransferase, low TP, low A/G and hyperbilirubinemia, respectively. The elevated inflammatory mediators (procalcitonin and C-reactive protein) and moderate dose of aspirin played a synthetic role in hepatic dysfunction secondary to KD. However, LFT presented no significant differences between infectious and noninfectious conditions. By a multivariate analysis, a lower albumin/globulin ratio (A/G, OR 13.50, 95% CI 3.944-46.23) served as an independent predictor of CAAs and had a sensitivity of 56.25%, and a specificity of 61.11% at a cutoff value of < 1.48. In conclusion, hepatic dysfunction is a common complication during the acute KD episode, characterized by elevated serum liver enzymes, hypoalbuminemia and hyperbilirubinemia. Systemic inflammation and aspirin, rather than infectious agents, are both the major contributors of hepatic dysfunction secondary to KD. A lower A/G serves as an independent predictor of CAAs.

19.
Zootaxa ; 4608(3): zootaxa.4608.3.12, 2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31717141

RESUMO

Ceraeocercus Uvarov, 1910 of Tettigoniinae; Drymadusini is receded in China for the first time. Ceraeocercus fuscipennis fuscipennis Uvarov, 1910 is documented. Morphology and male calling songs are described and illustrations are provided.


Assuntos
Ortópteros , Distribuição Animal , Animais , China , Masculino
20.
Sci Adv ; 5(11): eaax9800, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763456

RESUMO

Satellite observations of nitrogen dioxide (NO2) provide valuable information on the location and strength of NO x emissions, but spatial resolution is limited by horizontal transport and smearing of temporal averages due to changing wind fields. In this study, we map NO x emissions on high spatial resolution from TROPOMI observations of NO2 combined with wind fields based on the continuity equation. The divergence of horizontal fluxes proves to be highly sensitive for point sources like exhaust stacks. Thus, NO x emissions from individual power plants can be resolved and quantified even on top of considerably high urban pollution from the Saudi Arabian capital city Riyadh. This allows us to catalog NO x emissions from large point sources globally, as demonstrated for South Africa and Germany, with a detection limit of about 0.11 kg/s down to 0.03 kg/s for ideal conditions.

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