Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.569
Filtrar
1.
Food Chem ; 335: 127596, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32745840

RESUMO

The ciprofloxacin (CIP) abuse has caused many problems threatening to human health. Here, we design the quantum dot microsphere (QDM) based immunochromatographic quantitative CIP test strip: when the sample under detection contains CIP, the QDM-monoclonal antibody (mAb) probes bound with the CIP and cannot be captured by CIP-bovine serum albumin (BSA) conjugation dispersed on the T lines, reducing the fluorescence intensities. These test strips can provide a low detection limit of 0.05 ng/mL and a wide linear detection range from 0.1 ng/mL to 100 ng/mL in high sensitivity and accuracy as well as good selectivity, reproducibility and stability. Moreover, a smartphone based test strip reader with the size of 85 mm × 48 mm × 44 mm is also fabricated using 3-D printing to automatically and quantitatively detect CIP. The whole process of CIP detection can be finished within 15 min, but only cost ~1 RMB (10 cents).

3.
Methods Mol Biol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33058029

RESUMO

Mesenchymal stem/stromal cells (MSCs) show excellent therapeutic potentials in many preclinical studies and clinical trials. However, the clinical application of conventional tissue-derived MSCs faces challenges of limited scalability and high donor variations. To address these challenges, we established a protocol for deriving and characterizing MSCs from human induced pluripotent stem cells (iPSCs) with a theoretically limitless expandability. The iPSC-MSCs show biological properties comparable to or better than early passage bone marrow MSCs and can be scaled up to huge amounts with uniform properties.

4.
Head Neck ; 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33058368

RESUMO

BACKGROUND: To assess long-term donor site morbidity after radial forearm free (RFF) flap harvesting. METHODS: Enrolled patients were asked to complete the Disability of the Arm, Shoulder, and Hand (DASH) questionnaire and undergo wrist motion and hand strength examinations at different time points. The data were prospectively collected and retrospectively analyzed. RESULTS: The postoperative DASH score did not return to normal until 24 months after the operation. The mean postoperative wrist motion degree of flexion was significantly decreased compared to the preoperative level and returned to normal at 12 months after the operation. Similar trends were noted regarding extension, radial abduction, and ulnar abduction. The mean postoperative grip strength was significantly decreased compared to the preoperative level and remained dysfunctional at 24 months after the operation. A similar trend was also noted with regard to tip pinch and key pinch. CONCLUSION: The long-term negative effect on hand strength is sustained.

5.
Aging Ment Health ; : 1-7, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33021096

RESUMO

OBJECTIVES: Both cognitive reserve and modifiable-risk-factor profiles play a role in dementia incidence. We investigated whether cognitive reserve moderates the risk of dementia attributable to the modifiable-risk-factor profile. METHOD: We followed 2102 older individuals aged 65+ years recruited from the population-based longitudinal cohort CFAS Wales study, begun in 2011, and the follow-up wave completed in early 2016. Cognitive reserve was measured by combining educational level, occupation complexity, and engagement in social and cognitive activities in later life. Modifiable-risk-factor profile scores were based on depression, diabetes, smoking, physical activity, healthy diet, and drinking. The interactions between cognitive reserve indicators and modifiable-risk-factor profiles were assessed on multiplicative and additive scales. RESULTS: There is an additive interaction between the composite effect of cognitive reserve indicator and modifiable-risk-factor profile on dementia. In those with low cognitive reserve, the risk of dementia in participants with a favorable profile was significantly lower than in those with an unfavorable one (OR = 0.08, 95% CI = 0.02-0.27). CONCLUSION: Cognitive reserve significantly moderates the association between modifiable-risk-factor profiles and dementia.

6.
Artigo em Inglês | MEDLINE | ID: mdl-33046485

RESUMO

Hepatitis B virus (HBV) mRNA metabolism is dependent upon host proteins PAPD5 and PAPD7 (PAPD5/7). PAPD5/7 are cellular, non-canonical, poly(A) polymerases (PAP) whose main function is to oligoadenylate the 3' end of non-coding RNA (ncRNA) for exosome degradation. HBV seems to exploit these two ncRNA quality control factors for viral mRNA stabilization, rather than degradation. RG7834 is a small molecule compound that binds PAPD5/7 and inhibits HBV gene production in both tissue culture and animal study. We reported that RG7834 was able to destabilize multiple HBV mRNA species, ranging from the 3.5 kb pre-genomic/pre-core mRNAs to the 2.4/2.1 kb HBs mRNAs, except for the smallest 0.7 kb HBx mRNA. Compound induced HBV mRNA destabilization was initiated by a shortening of poly(A) tail followed by an accelerated degradation process in both the nucleus and cytoplasm. In cells expressing HBV mRNA, both PAPD5/7 were found to be physically associated with the viral RNA, and the polyadenylating activities of PAPD5/7 were susceptible to RG7834 repression in biochemical assay. Moreover, in PAPD5/7 double knockout cells, viral transcripts with regular length of poly(A) sequence could be initially synthesized but became shortened in hours suggesting that participation of PAPD5/7 in RNA 3' end processing, either during adenosine oligomerization or afterwards, is crucial for RNA stabilization.

7.
Cancer Res ; 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32998998

RESUMO

Irreversible hypofunction of salivary glands is a common side effect of radiotherapy for head-and-neck cancer and is difficult to remedy. Recent studies indicate that transient activation of Hedgehog signaling rescues irradiation-impaired salivary function in animal models, but the underlying mechanisms are largely unclear. Here we show in mice that activation of canonical Gli-dependent Hedgehog signaling by Gli1 gene transfer is sufficient to recover salivary function impaired by irradiation. Salivary gland cells responsive to Hedgehog/Gli signaling comprised small subsets of macrophages, epithelial cells and endothelial cells, and their progeny remained relatively rare long after irradiation and transient Hedgehog activation. Quantities and activities of salivary gland resident macrophages were substantially and rapidly impaired by irradiation and restored by Hedgehog activation. Conversely, depletion of salivary gland macrophages by clodronate liposomes compromised the restoration of irradiation-impaired salivary function by transient Hedgehog activation. Single cell RNA sequencing and qRT-PCR of sorted cells indicated that Hedgehog activation greatly enhances paracrine interactions between salivary gland resident macrophages, epithelial progenitors, and endothelial cells through Csf1, Hgf, and C1q signaling pathways. Consistently, expression of these paracrine factors and their receptors in salivary glands decreased following irradiation but were restored by transient Hedgehog activation. These findings reveal that resident macrophages and their pro-repair paracrine factors are essential for the rescue of irradiation-impaired salivary function by transient Hedgehog activation and are promising therapeutic targets of radiotherapy-induced irreversible dry mouth.

8.
Adv Healthc Mater ; : e2001289, 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33052037

RESUMO

Lipid nanoemulsions (NEs), owing to their controllable size (20 to 500 nm), stability and biocompatibility, are now frequently used in various fields, such as food, cosmetics, pharmaceuticals, drug delivery, and even as nanoreactors for chemical synthesis. Moreover, being composed of components generally recognized as safe (GRAS), they can be considered as "green" nanoparticles that mimic closely lipoproteins and intracellular lipid droplets. Therefore, they attracted attention as carriers of drugs and fluorescent dyes for both bioimaging and studying the fate of nanoemulsions in cells and small animals. In this review, the composition of dye-loaded NEs, methods for their preparation, and emerging biological applications are described. The design of bright fluorescent NEs with high dye loading and minimal aggregation-caused quenching (ACQ) is focused on. Common issues including dye leakage and NEs stability are discussed, highlighting advanced techniques for their characterization, such as Förster resonance energy transfer (FRET) and fluorescence correlation spectroscopy (FCS). Attempts to functionalize NEs surface are also discussed. Thereafter, biological applications for bioimaging and single-particle tracking in cells and small animals as well as biomedical applications for photodynamic therapy are described. Finally, challenges and future perspectives of fluorescent NEs are discussed.

9.
Zootaxa ; 4860(4): zootaxa.4860.4.6, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33055882

RESUMO

The genus Decticus Serville contains sixteen known species and subspecies, those widely distributed in the Palaearctic, among which three species and subspecies have been recorded in China. Morphological features in specimens of Decticus are variable. Stable characteristics and reliable methods for distict the species and subspcies are required. The geometric morphometrics was conducted to explore morphological variance on structure of right tegmen of Decticus specimens based on 30 landmarks, as well as qualitative analysis on variances of body size, male stridualtory apparatus and genital characters. The male calling songs structures, if available, can be compared and identified species usefully. The results indicate that the analyzed samples suggest two species, D. albifrons (Fabricius, 1775) and D. verrucivorus (Linnaeus, 1758). D. verrucivorus includes four subspecies, D. v. verrucivorus (Linnaeus, 1758), D. v. crassus Götz, 1970 (a newly recorded subspecies from China), and two new subspecies, D. v. latipennis ssp. n. and D. v. sayram ssp. n.. The songs of D. albifrons and D. v. verrucivorus, D. v. crassus are composed of numerous single chirps. One chirp of D. albifrons consists of one low-amplitude and short-duration syllable, and one high-amplitude and long-duration syllable. One chirp of D. v. verrucivorus consists of five syllables with different amplitudes. One chirp of D. v. crassus consists of five or six syllables with different amplitudes. Illustrations of necessary morphological and bioacoustical characters are presented.


Assuntos
Ortópteros , Distribuição Animal , Animais , Tamanho Corporal , China , Masculino
10.
BMC Surg ; 20(1): 243, 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33069222

RESUMO

BACKGROUND: To compare the efficacy of total and conventional laparoscopic hepaticojejunostomy (TLH and CLH) in children with choledochal cysts (CDCs). METHODS: Data from patients undergoing TLH and CLH between August 2017 and December 2018 were retrospectively analyzed. Intraoperative blood loss, time for jejunum-cojejunum anastomosis, time to oral intake, postoperative hospital stay, hospitalization expenses, and postoperative complications were compared. RESULTS: All 55 patients (TLH = 30, CLH = 25) were successfully treated without conversion to open surgery. In the TLH and CLH groups, the time to oral intake was 3.57 ± 0.19 d and 4.56 ± 0.27 d, respectively (t = 3.07, P < 0.01), the postoperative hospital stay was 5.50 ± 0.28 d and 7.00 ± 0.74 d (t = 2.03, P < 0.05), and the hospitalization expenses were CNY 40,085 ± 2447 and CNY 26,084 ± 2776 (t = 3.79, P < 0.001). There were no significant differences in intraoperative blood loss (9.57 ± 3.28 ml vs 8.2 ± 1.13 ml, t = 0.37, P = 0.72) or time for jejunum-cojejunum anastomosis (80.5 ± 2.46 min vs 75.00 ± 2.04 min, t = 1.68, P = 0.10). The median follow-up periods of the TLH and CLH groups were 17 and 16 months, respectively. Overall complication rates were comparable between the two groups (10% vs 8%, χ2 = 0.07, P = 0.79). CONCLUSIONS: TLH in children with CDCs has the advantages of rapid gastrointestinal functional recovery and a short hospitalization. However, hospitalization is relatively expensive.

11.
J Environ Sci (China) ; 98: 169-178, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33097149

RESUMO

Diclofenac (DCF), a widely used non-steroidal anti-inflammatory, reacted readily with birnessite under mild conditions, and the pseudo first order kinetic constants achieved 8.84 × 10-2 hr-1. Five products of DCF including an iminoquinone product (2,5-iminoquinone-diclofenac) and four dimer products were observed and identified by tandem mass spectrometry during the reaction. Meanwhile, 2,5-iminoquinone-diclofenac was identified to be the major product, accounting for 83.09% of the transformed DCF. According to the results of spectroscopic Mn(III) trapping experiments and X-ray Photoelectron Spectroscopy, Mn(IV) contained in birnessite solid was consumed and mainly converted into Mn(III) during reaction process, which proved that the removal of DCF by birnessite was through oxidation. Based on the identified products of DCF and the changes of Mn valence state in birnessite solid, a tentative transformation pathway of DCF was proposed.

12.
Biomed Pharmacother ; 132: 110855, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33059257

RESUMO

Ischemic diseases, such as ischemic heart diseases and ischemic stroke, are the leading cause of death worldwide. Angiogenic therapy is a wide-ranging approach to fighting ischemic diseases. However, compared with anti-angiogenesis therapy for tumors, less attention has been paid to therapeutic angiogenesis. Recently, Traditional Chinese medicine (TCM) has garnered increasing interest for its definite curative effect and low toxicity. A growing number of studies have reported that TCM formulas, extracts, and compounds from herbal medicines exert pro-angiogenic activity, which has been confirmed in a few clinical trials. For comprehensive analysis of relevant literature, global and local databases including PubMed, Web of Science, and China National Knowledge Infrastructure were searched using keywords such as "angiogenesis," "neovascularization," "traditional Chinese medicine," "formula," "extract," and "compound." Articles were chosen that are closely and directly related to pro-angiogenesis. This review summarizes the pro-angiogenic activity and the mechanism of TCM formulas, extracts, and compounds; it delivers an in-depth understanding of the relationship between TCM and pro-angiogenesis and will provide new ideas for clinical practice.

13.
J Hazard Mater ; : 124122, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33092885

RESUMO

The adsorptive removal of persistent organic pollutants (POPs) is reckoned as a simple, convenient, and practical technology, especially in decentralized systems and remote areas. For this purpose, it is important to design new adsorbents, with controllable structure and convenient shape, for the highly efficient removal of POPs. In this study, we describe a strategy for film-like water purifier, prepared by loading cyclodextrin (CD) oligomer onto the ultrafine nanofibers of 3D bacterial cellulose. The optimum product exhibits remarkable removal ability toward various target pollutants such as phenol, bisphenol A (BPA), glyphosate and 2,4-Dichlorophenol (2,4-DCP), with capacities higher than most adsorbents including porous carbon based materials reported previously. Moreover, our sample demonstrated stable adsorption ability over a broad pH range and under more complex water conditions, and more importantly excellent reusability. A rough cost analysis highlights the commercial potential of our sample. We reckon our study provides new insight for the design of adsorbent with high yet stable adsorption ability and controllable structure. Furthermore, the product can be used to treat actual sewage with its convenient film-like shape and excellent performance, which improves its potential in complex systems and large-scale applications.

14.
Plant Dis ; 2020 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-33100150

RESUMO

Blackleg, caused by Leptosphaeria maculans, is a major disease of canola in Canada, Australia, and Europe. For effective deployment of resistant varieties and disease management, it is crucial to understand the population structure of L. maculans. In this study, we analyze L. maculans isolates from commercial fields in western Canada from 2014 to 2016 for the presence and frequency of avirulence (Avr) genes. A total of 1, 584 isolates was examined for the presence of Avr genes AvrLm1, AvrLm2, AvrLm3, AvrLm4, AvrLm6, AvrLm7, AvrLm9, AvrLepR1, AvrLepR2, and AvrLmS using a set of differential host genotypes carrying known resistance genes and a PCR assay. Several Avr genes showed a higher frequency in the pathogen population, such as AvrLm6 and AvrLm7, which were present in >90% of isolates; while AvrLm3, AvrLm9 and AvrLepR2 showed frequencies of <10%. A total of 189 races (different combinations of Avr genes) were detected, with Avr-2-4-6-7-S, Avr-1-4-6-7 and Avr-2-4-6-7 as the three predominant races. When the effect of crop rotation was assessed, only a three-year rotation showed a significantly higher frequency of AvrLm2, relative to shorter rotations. This study provides the information for producers to select effective canola varieties for blackleg management, and for breeders to deploy new R genes in disease resistance breeding in the western Canada region.

15.
Mol Vis ; 26: 670-678, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33088171

RESUMO

Purpose: To identify the genetic cause in a four-generation Chinese family with Axenfeld-Rieger syndrome (ARS). Methods: The family members received clinical examinations of the eye, tooth, periumbilical skin, and heart. Sanger sequencing and whole-exome sequencing (WES) were performed to screen potential mutations. The genomic deletion region around the PITX2 gene was estimated from single nucleotide polymorphism (SNP) data from WES and then confirmed with "quantitative PCR (qPCR) using a set of primers. The DNA breakpoint was further identified with long-range PCR and Sanger sequencing. Results: Symptoms including anterior segment dysplasia of the eye (iris dysplasia, multiple pupils, and posterior embryotoxon), dental dysplasia, and periumbilical skin redundancy were present in all of the affected individuals. Three of them had glaucoma. Corneal abnormalities (inferior sclerocornea, corneal endothelial dystrophy, and central corneal scar) were seen in most of the affected individuals. Cataract, limited eye movement, electrocardiographic abnormalities, intellectual disability, and recurrent miscarriages were observed in some of the affected individuals. No mutations in the coding and exon-intron adjacent regions of the PITX2 and FOXC1 genes were identified with Sanger sequencing. According to the SNP data from WES, we suspected that there might be a deletion region (at most 1.6 Mb) around the PITX2 gene. With the use of qPCR and long-range PCR, we identified a 53,840 bp deletion (chr4: 111,535,454-111,588,933) spanning PITX2 and PANCR. The genomic deletion cosegregated with the major ARS symptoms observed in the family members. Conclusions: With the help of WES, qPCR, and long-range PCR, we identified a genomic deletion encompassing PITX2 and the adjacent noncoding gene PANCR in a Chinese family with ARS. The clinical features of the affected individuals are reported. This work may broaden understanding of the phenotypic and mutational spectrums related to ARS.

16.
Technol Health Care ; 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33074204

RESUMO

BACKGROUND: The traditional meal assistance robots use human-computer interaction such as buttons, voice, and EEG. However, most of them rely on excellent programming technology for development, in parallelism with exhibiting inconvenient interaction or unsatisfactory recognition rates in most cases. OBJECTIVE: To develop a convenient human-computer interaction mode with a high recognition rate, which allows users to make the robot show excellent adaptability in the new environment without programming ability. METHODS: A visual interaction method based on deep learning was used to develop the feeding robot: when the camera detects that the user's mouth is open for 2 seconds, the feeding command is turned on, and the feeding is temporarily conducted when the eyes are closed for 2 seconds. A programming method of learning from the demonstration, which is simple and has strong adaptability to different environments, was employed to generate a feeding trajectory. RESULTS: The user is able to eat independently through convenient visual interaction, and it only requires the caregiver to drag and teach the robotic arm once in the face of a new eating environment.

17.
Cancer Biomark ; 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-33074217

RESUMO

Gastric cancer (GC) remains poor prognosis and survival issues due to the resistance of chemotherapies, such as cisplatin. The long non-coding RNA small nucleolar RNA host gene 7 (lncRNA-SNHG7) is known as an oncogenic molecule in diverse cancers. Here, we demonstrate that SNHG7 was significantly upregulated in gastric cancer and positively correlated with cisplatin resistance of gastric cancer cells that SNHG7 was significantly upregulated in cisplatin resistant cells. Silencing SNHG7 dramatically sensitized cisplatin resistant cells. In contrast, a negative correlation between lncRNA-SNHG7 and miR-34a was found that miR-34a was downregulated in gastric cancer patient tissues and significantly sensitized cisplatin resistant gastric cancer cells. Intriguingly, bioinformatical analysis indicated miR-34a has putative biding site for SNHG7 and such negative association between SNHG7 and miR-34a was verified in gastric cancer tissues. The cisplatin resistant cells displayed increased glycolysis rate and SNHG7 promoted cellular glycolysis rate of gastric cancer cells. Luciferase assay illustrated LDHA, a glycolysis enzyme, was the direct target of miR-34a. Importantly, inhibiting SNHG7 successfully suppressed LDHA expressions and sensitized cisplatin resistant cells and such inhibitory effects could be recovered by further anti-miR-34a. These findings suggest an important regulator mechanism for the SNHG7-mediated cisplatin resistance via miR-34a/LDHA-glycolysis axis.

18.
J Zhejiang Univ Sci B ; 21(10): 779-795, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33043644

RESUMO

Verticillin A is a diketopiperazine compound which was previously isolated from Amanita flavorubescens Alk (containing parasitic fungi Hypomyces hyalines (Schw.) Tul.). Here, we initially found, by wound healing assay and Transwell assay in vitro, that verticillin A possesses an inhibitory effect against the migration and invasion of the human colon cancer cell. Subsequently, c-mesenchymal-epithelial transition factor (c-Met) was identified as a molecular target of verticillin A by screening key genes related to cell migration. Verticillin A-mediated c-Met suppression is at the transcriptional level. Further study demonstrated that verticillin A suppressed c-MET phosphorylation and decreased c-MET protein level. In addition, verticillin A inhibited the phosphorylation of c-MET downstream molecules including rat sarcoma (Ras)-associated factor (Raf), extracellular signal-regulated kinase (ERK), and protein kinase B (AKT). Overexpression of Erk partially reversed the verticillin A-mediated anti-metastasis action in the human colon cancer cell. More importantly, verticillin A also inhibited cancer cell metastasis in vivo. Thus, verticillin A can significantly inhibit the migration and invasion of colon cancer cells by targeting c-Met and inhibiting Ras/Raf/mitogen-activated extracellular signal-regulated kinase (MEK)/ERK signaling pathways. Therefore, we determined that verticillin A is a natural compound that can be further developed as an anti-metastatic drug in human cancers.

19.
Neuropeptides ; 84: 102098, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33069139

RESUMO

In the spinal nervous system, the expression of galanin (GAL) and galanin receptors (GALRs) that play important roles in the transmission and modulation of nociceptive information can be affected by nerve injury. However, in the trigeminal nervous system, the effects of trigeminal nerve injury on the expression of GAL are controversy in the previous studies. Besides, little is known about the effects of trigeminal nerve injury on the expression of GALRs. In the present study, the effects of trigeminal nerve injury on the expression of GAL and GALRs in the rat trigeminal ganglion (TG) were investigated by using quantitative real-time reverse transcription-polymerase chain reaction and immunohistochemistry. To identify the nerve-injured and nerve-uninjured TG neurons, activating transcription factor 3 (ATF3, the nerve-injured neuron marker) was stained by immunofluorescence. The levels of GAL mRNA in the rostral half and caudal half of the TG dramatically increased after transection of infraorbital nerve (ION) and inferior alveolar nerve (IAN), respectively. Immunohistochemical labeling of GAL and ATF3 revealed that GAL level was elevated in both injured and adjacent uninjured small and medium-sized TG neurons after ION/IAN transection. In addition, the levels of GAL2R-like immunoreactivity were reduced in both injured and adjacent uninjured TG neurons after ION/IAN transection, while levels of GAL1R and GAL3R-like immunoreactivity remained unchanged. Furthermore, the number of small to medium-sized TG neurons co-expressing GAL- and GAL1R/GAL2R/GAL3R-like immunoreactivity was significantly increased after ION/IAN transection. In line with previous studies in other spinal neuron systems, these results suggest that GAL and GALRs play functional roles in orofacial neuropathic pain and trigeminal nerve regeneration after trigeminal nerve injury.

20.
Medicine (Baltimore) ; 99(41): e22580, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33031307

RESUMO

RATIONALE: Mycobacterium tuberculosis (TB) remains a serious threat in developing countries. Primary isolated hepatic tuberculosis is extremely rare. Because of its non-specific imaging features, noninvasive preoperative imaging diagnosis of isolated hepatic tuberculoma remains challenging. PATIENT CONCERNS: A 48-year-old man was admitted to our hospital due for suspected liver neoplasm during health examination. DIAGNOSES: The tests for blood, liver function, and tumor markers were within normal range. Preoperative ultrasonography (US) showed a hypoechoic lesion with a longitudinal diameter of 2.5 cm in segment six of liver. It exhibited early arterial phase hyperenhancement and late arterial phase rapid washout in contrast-enhanced US. It demonstrated hyperintensity in T2-weighted magnetic resonance imaging and partly restricted diffusion in diffusion-weighted imaging. For this nodule, the preoperative diagnosis was small hepatocellular carcinoma (HCC). INTERVENTIONS: Laparoscopic hepatectomy was performed. Intraoperative extensive adhesion in the abdominal cavity and liver was found. The lesion had undergone expansive growth. OUTCOMES: Microscopically, a granuloma with some necrosis was detected. With both acid-fast staining and TB fragment polymerase chain reaction showing positive results, TB was the final histology diagnosis. After surgery, the patient declined any anti-TB medication. During the follow-up, he had no symptoms. In the sixth month after surgery, he underwent an upper abdominal US. It showed no lesions in the liver. LESSONS: Because of non-specific imaging findings and non-specific symptoms, a diagnosis of isolated hepatic TB is difficult to make, especially for small lesions. A diagnosis of HCC should be made cautiously when small isolated lesions in the liver are encountered, especially in patients without a history of hepatitis and with negative tumor markers.


Assuntos
Tuberculose Hepática/diagnóstico por imagem , Tuberculose Hepática/cirurgia , Carcinoma Hepatocelular/diagnóstico por imagem , Meios de Contraste , Diagnóstico Diferencial , Hepatectomia , Humanos , Neoplasias Hepáticas/diagnóstico por imagem , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Ultrassonografia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA