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1.
PLoS Biol ; 19(9): e3001386, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34499638

RESUMO

Plasmodium falciparum, the deadliest causal agent of malaria, caused more than half of the 229 million malaria cases worldwide in 2019. The emergence and spreading of frontline drug-resistant Plasmodium strains are challenging to overcome in the battle against malaria and raise urgent demands for novel antimalarial agents. The P. falciparum formate-nitrite transporter (PfFNT) is a potential drug target due to its housekeeping role in lactate efflux during the intraerythrocytic stage. Targeting PfFNT, MMV007839 was identified as a lead compound that kills parasites at submicromolar concentrations. Here, we present 2 cryogenic-electron microscopy (cryo-EM) structures of PfFNT, one with the protein in its apo form and one with it in complex with MMV007839, both at 2.3 Å resolution. Benefiting from the high-resolution structures, our study provides the molecular basis for both the lactate transport of PfFNT and the inhibition mechanism of MMV007839, which facilitates further antimalarial drug design.

2.
Food Res Int ; 148: 110613, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34507757

RESUMO

Oxygen involved fermentation is generally recognized as the critical process for the formation of quality of black tea. However, the specific role of oxygen plays in taste-related metabolites' alteration has not been illustrated clearly. In the present work, a series of fermentation systems with different oxygen concentrations were used to investigate the mechanism of the effects of oxygen on the quality and nonvolatile metabolites in black tea. The results showed that oxygen-enriched fermentation significantly improved the taste of black tea. And sixty-six metabolites, including catechins, theaflavins (TFs), proanthocyanidins, amino acids, flavonoid glycosides, and phenolic acids, were significantly different in the black teas fermented by three oxygen concentrations. Meanwhile, a 10-30% decrease in catechins, flavonoid glycosides and phenolic acids and a 5% increase in TFs, glutamate and glutamine in oxygen-enriched group, when compared to the control group, reduced astringency and bitterness and enhanced umami intensity. Furthermore, increased oxygen concentrations promoted the oxidation of catechins, flavonoid glycosides and some phenolic acids. And catechins oxidation in turn could accelerate the degradation of amino acids to form volatile aldehydes and also promote phenolic acids oxidation. Our results reveal the potential role of oxygen plays in the metabolites' alteration in black tea during fermentation, which gives a new insight into understanding the quality formation of black tea.

3.
Phytochemistry ; 192: 112927, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34492546

RESUMO

Bisabolane-type sesquiterpenoids, a class of monocyclic sesquiterpenoids, are widely distributed in nature and have a variety of biological activities. To provide a reference for the further research and development of these compounds, the phytochemical and biological properties of natural bisabolane-type sesquiterpenoids (356 compounds in total) isolated between 1985 and 2020 from 24 families, primarily Compositae, Zingiberaceae, Aspergillaceae, Halichondriidae, and Aplysiidae were reviewed. In vitro and in vivo studies have indicated that antibacterial, anti-inflammatory, and cytotoxic effects are the most commonly reported pharmacological properties of bisabolane-type sesquiterpenoids. Owing to their extensive significant effects, a lot of traditional medicines containing this type of compounds have been used for a long history. Thus, bisabolane-type sesquiterpenoids are a rich source of important natural products, which show great potential for the development of new drugs.

4.
ACS Sens ; 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34529414

RESUMO

Rapid and sensitive detection of clinical biomolecules in a multiplexed fashion is of great importance for accurate diagnosis of diseases. Mass spectrometric (MS) approaches are exceptionally suitable for clinical analysis due to its high throughput, high sensitivity, and reliable qualitative and quantitative capabilities. To break through the bottleneck of MS technique for detecting high-molecular-weight substances with low ionization efficiency, the concept of mass spectrometric biosensing has been put forward by adopting mass spectrometric chips to recognize the targets and mass spectrometry to detect the signals switched by the recognition. In this review, the principle of mass spectrometric sensing, the construction of different mass tags used for biosensing, and the typical combination mode of mass spectrometric imaging (MSI) technique are summarized. Future perspectives including the design of portable matching platforms, exploitation of novel mass tags, development of effective signal amplification strategies, and standardization of MSI methodologies are proposed to promote the advancements and practical applications of mass spectrometric biosensing.

5.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120372, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34530198

RESUMO

Photo-reduction of silver oxide and light-induced Ag nanoparticle (NP) generations have been applied for Surface-enhanced Raman spectroscopy (SERS) substrate fabricated for years. In this paper, we demonstrate a general method to enhance the SERS activity of conventional Ag NPs-based SERS substrates by performing Raman scattering measurement in a nitrogen ambient after a period of laser irradiation (photoactivation). The Raman characteristic peak intensity of carbonaceous impurities adsorbed on the surfaces of Ag NPs display an additional enhancement of 93 times after photoactivation in nitrogen ambient. A 3-fold extra Raman gain enhancement is also observed in the nitrogen-protected SERS measurement of R6G molecules. The extra SERS enhancement is attributed to the sub-nanometer scale near-field coupling between the Ag NPs and the photo-generated Ag clusters in the surface oxide layer of Ag NPs. This model is verified through the finite-difference time-domain (FDTD) simulations.

6.
J Clin Pediatr Dent ; 45(4): 278-283, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34534298

RESUMO

BACKGROUND/OBJECTIVE: To retrospectively analyze the characteristics, tendencies, and success rates of dental treatments for severe early childhood caries (SECC) under general anesthesia (GA) in in northwest China. STUDY DESIGN: Children diagnosed with SECC were included in this retrospective study. From January 2015 to December 2018, they received dental treatment under GA at Affiliated Stomatology Hospital of Xi'an Jiaotong University. Demographic information, caries status and treatment characteristics were collected from electronic medical record system. Success rates of different treatments at 6-month and 12-month follow up were also analyzed. RESULTS: A total of 846 children (477 male, 369 female) received dental treatment under GA. The case number was increased from 148 in 2015 to 278 in 2018. There were 81.2% and 70.8% of the children participated the 6-month and 12-month follow up. SSC and pulpotomy was the most successful restorations and pulp therapy, with the success rate of 97.09% and 93.98% in 12-month follow up, respectively. The use of crown restorations (including composite resin crown and stainless steel crown) and pulp reservation therapies (including indirect pulp therapy and pulpotomy) were significantly increased while composite resin filling and pulpectomy decreased during 2015 to 2018. CONCLUSION: There has been an increasing demand for dental treatment under GA for children with SECC in northwest China, with a trend toward younger ages. With better clinical outcomes, crown restorations and pulp reservation therapies were the fastest-growing treatments under GA.

7.
Biomed Microdevices ; 23(4): 44, 2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34536144

RESUMO

Saccharomyces cerevisiae is well-known in the baking and brewing industries and always used for the preparation of probiotics, especially its subtype, Saccharomyces boulardii, to prevent and treat various diarrhea and intestinal diseases. However, case reports on the side effects of a wide range of serious infections for the elderly, immunocompromised and critically ill patients after treatment with the S. cerevisiae have been increasing in recent years. The existing diagnose methods of the invasive S. cerevisiae infections in clinical, especially, the key step of the method-cell isolation, is time-consuming that always miss timey diagnose and early prevention. Here, we propose a new automatic micromanipulation method to label-free rapid isolation of S. cerevisiae based on the optically-induced dielectrophoresis (ODEP) technology, combining with image processing and recognition. S. cerevisiae is firstly identified by the image recognition method and then, automatically captured and moved to the target location by designing optical patterns. The results indicate the method can flexibly and automatically manipulate multiple S. cerevisiae cells simultaneously, such as, arranging S. cerevisiae cells, moving an array of the cells at any directions, aggregating the cells, and separating S. cerevisiae from the solution mixed with impurities. This work represents a step toward the use of automatic micromanipulation of ODEP technology to automatically and rapidly isolate S. cerevisiae for the detection of the invasive S. cerevisiae infections.

8.
Orthop Surg ; 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34523790

RESUMO

OBJECTIVE: The study introduced uniportal-bichannel spinal endoscopic system (UBiSES) and explored the feasibility of applying UBiSES to conduct lumbar foraminoplasty in percutaneous endoscopic transforaminal discectomy (PETD). METHODS: This is a cohort study. 36 patients confirmed as L5/S1 lumbar disc herniation (LDH) in our hospital from March, 2019 to November, 2019 were enrolled. 36 patients were divided into two groups named the UBiSES group (n = 18, male: female = 8:10) and the TESSYS group (n = 18, male: female = 10:8). The average age of the UBiSES group and the TESSYS group were 40.94 ± 12.39 years old and 39.78 ± 13.02 years old respectively. PETD via uniportal-bichannel foraminoplasty assisted by UBiSES was adopted on the UBiSES group while PETD via conventional foraminoplasty was performed on the TESSYS group. One experienced surgeon with more than 4000 cases of lumbar surgery performed PETD on all patients. The demographic data, the duration of working cannula placement (minutes), decompression time (minutes), radiation exposure time (seconds), complications, Visual Analogue Scale (VAS), Oswestry Disability Index (ODI) scores and modified MacNab criteria were recorded and analyzed. The magnetic resonance imaging (MRI) and computed tomography (CT) were conducted to evaluate the radiographic improvement. RESULTS: PETD via lumbar foraminoplasty was successfully performed in all cases. The follow-up points were 3 months, 6 months, and 12 months. The average follow-up period of all patients was 15.78 ± 2.29 months. There was no statistic difference in age (P = 0.81), sex (P = 0.51) and follow-up (P = 0.14) between two groups. The duration of working cannula placement was 19.08 ± 2.30 min in the UBiSES group and 24.90 ± 4.71 min in the TESSYS group and there was significant difference between two groups (P < 0.05). There was no statistic difference in decompression time between the UBiSES group (44.18 ± 5.70 min) and the TESSYS group (47.46 ± 5.96 min) (P = 1.70). The radiation exposure time was 28.00 ± 4.70 s in the UBiSES group and 40.50 ± 5.73 s in the TESSYS group respectively, and has significant difference between two groups (P < 0.05). Furthermore, there was significant different in the duration of working cannula placement and radiation exposure time in male or female between the UBiSES group and the TESSYS group (P < 0.05). For male or female, no difference observed in decompression time and follow-up period between two groups. Postoperative VAS of low back and leg at every follow-up point (1 day, 3 months, 6 months, 12 months) was improved significantly in both groups compared with their preoperative VAS (P < 0.05). The postoperative ODI (3 months, 6 months, 12 months) has decreased significantly in both the UBiSES group and the TESSYS group compared with their preoperative ODI (P < 0.05). 94.44% patients received an excellent or good recovery in the UBiSES group and 88.89% for the TESSYS group. There was no poor result reported in both groups. The radiographic images showed satisfactory foraminoplasty and sufficient decompression of nerve in both groups. No postoperative complications were observed during follow-ups in the UBiSES group. Two patients in the TESSYS group experienced postoperative dysesthesia and the symptom was disappeared in 5 days and 7 days respectively with dexamethasone and neurotrophic drugs treatment. CONCLUSIONS: The original designed UBiSES could effectively and safely enlarge the foramen with an extensive surgical view and space under full-time and real-time visualization and get satisfactory efficacy.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34490489

RESUMO

Stormwater runoff samples were collected from five different land use sites (gas station, city road, campus, park, and residential) in a precipitation event on May 22nd, 2017, from a small suburban area (5 km × 2 km) of the city of Beijing, China. There were 72 types of semi-volatile organic compounds (SVOCs) found in these runoff samples, including 33 types of monocyclic aromatic hydrocarbons (MAHs), 22 types of polycyclic aromatic hydrocarbons (PAHs), 6 types of phthalate esters (PAEs), 9 types of pesticides and 2 types of polychlorinated biphenyls (PCBs). Especially, 26 types of SVOCs (7 MAHs, 9 PAHs, 5 PAEs, and 5 pesticides) were detected in all water samples. SVOCs concentrations were higher in the samples from gas station and city road, and lower in runoff from campus, park and residential sites. The change in the ratio of anthracene to anthracene plus phenanthrene (ANT/(ANT + PHE)) in this study, reflected the importance of PAH source and land use. Di-2-ethylhexyl phthalate (DEHP) and di-n-butyl phthalate, are two of the phthalate esters 100% detected in the runoff samples. The city road runoff DEHP concentrations recorded the highest values (> 6000 ng/L), however, were still less than those wastewater DEHP pollutants measured in developed countries (e.g. UK, Canada, Finland, etc.). One-way ANOVA analysis in this study, showed that land use could significantly influence 23 SVOCs in the runoff samples, whereas the runoff SVOCs in different precipitation period showed no statistical changes in the five sites, and presented a general temporal trends "high (beginning)-low (middle period)-little raising (ending)". The findings in this study could be used in municipal management of wastewater collection and treatment.

10.
BMC Plant Biol ; 21(1): 408, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34493199

RESUMO

BACKGROUND: Mung bean (Vigna radiata) is a warm-season legume crop and belongs to the papilionoid subfamily of the Fabaceae family. China is the leading producer of mung bean in the world. Mung bean has significant economic and health benefits and is a promising species with broad adaptation ability and high tolerance to environmental stresses. OSCA (hyperosmolality-gated calcium-permeable channel) gene family members play an important role in the modulation of hypertonic stress, such as drought and salinity. However, genome-wide analysis of the OSCA gene family has not been conducted in mung bean. RESULTS: We identified a total of 13 OSCA genes in the mung bean genome and named them according to their homology with AtOSCAs. All the OSCAs were phylogenetically split into four clades. Phylogenetic relationship and synteny analyses showed that the VrOSCAs in mung bean and soybean shared a relatively conserved evolutionary history. In addition, three duplicated VrOSCA gene pairs were identified, and the duplicated VrOSCAs gene pairs mainly underwent purifying selection pressure during evolution. Protein domain, motif and transmembrane analyses indicated that most of the VrOSCAs shared similar structures with their homologs. The expression pattern showed that except for VrOSCA2.1, the other 12 VrOSCAs were upregulated under treatment with ABA, PEG and NaCl, among which VrOSCA1.4 showed the largest increased expression levels. The duplicated genes VrOSCA2.1/VrOSCA2.2 showed divergent expression, which might have resulted in functionalization during subsequent evolution. The expression profiles under ABA, PEG and NaCl stress revealed a functional divergence of VrOSCA genes, which agreed with the analysis of cis-acting regulatory elements in the promoter regions of VrOSCA genes. CONCLUSIONS: Collectively, the study provided a systematic analysis of the VrOSCA gene family in mung bean. Our results establish an important foundation for functional and evolutionary analysis of VrOSCAs and identify genes for further investigation of their ability to confer abiotic stress tolerance in mung bean.

11.
J Hematol Oncol ; 14(1): 140, 2021 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-34493319

RESUMO

BACKGROUND: In the absence of randomized studies directly comparing chimeric antigen receptor T cell therapies, this study used matching-adjusted indirect comparisons (MAIC) to evaluate the comparative efficacy and safety of lisocabtagene maraleucel (liso-cel) versus axicabtagene ciloleucel (axi-cel) in patients with relapsed or refractory large B cell lymphoma (LBCL). METHODS: Primary data sources included individual patient data from the TRANSCEND NHL 001 study (TRANSCEND [NCT02631044]; N = 256 for efficacy set, N = 269 for safety set) for liso-cel and summary-level data from the ZUMA-1 study (NCT02348216; N = 101 for efficacy set, N = 108 for safety set) for axi-cel. Inter-study differences in design, eligibility criteria, baseline characteristics, and outcomes were assessed and aligned to the extent feasible. Clinically relevant prognostic factors were adjusted in a stepwise fashion by ranked order. Since bridging therapy was allowed in TRANSCEND but not ZUMA-1, the initial efficacy and safety analyses included bridging therapy use as a matching factor (TRANSCEND patients who received bridging therapy were removed). Subsequent sensitivity analyses excluded this matching factor. RESULTS: The initial analysis showed similar MAIC-weighted efficacy outcomes between TRANSCEND and ZUMA-1 for overall and complete response rates (odds ratio [95% confidence interval (CI)], 1.40 [0.56-3.49] and 1.21 [0.56-2.64], respectively) and for overall survival and progression-free survival (hazard ratio [95% CI], 0.81 [0.44-1.49] and 0.95 [0.58-1.57], respectively). MAIC-weighted safety outcomes favored liso-cel, with significantly lower odds of all-grade and grade ≥ 3 cytokine release syndrome (odds ratio [95% CI], 0.03 [0.01-0.07] and 0.08 [0.01-0.67], respectively) and study-specific neurological events (0.16 [0.08-0.33] and 0.05 [0.02-0.15], respectively). Efficacy and safety outcomes remained similar in sensitivity analyses, which did not include use of bridging therapy as a matching factor. CONCLUSIONS: After matching and adjusting for clinically relevant prognostic factors, liso-cel demonstrated comparable efficacy and a more favorable safety profile compared with axi-cel in patients with third- or later-line relapsed or refractory LBCL. TRIAL REGISTRATION: NCT02631044 and NCT02348216.

12.
Chemosphere ; 282: 131092, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34470156

RESUMO

Remediation of heavy metal contaminated soil with appropriate washing agents is crucial to the decline in the harmfulness of contaminated soil by heavy metals to the environment and human health. In this study, citric acid (CA) and water-soluble chitosan (WSCS) as natural and degradable washing agents were used to remove Zn in the soil by two-step washing method. Results indicated that the two-step washing with CA and WSCS were found to be suitable for the removal of Zn from the contaminated soils, which significantly decreased the total concentration of Zn in the soil. After the remediation process with two-step soil washing, the contents of Zn in different chemical species decreased, especially for the carbonate-bound fraction. Therefore, the two-step soil washing with CA and WSCS was advisable for the remediation of Zn-contaminated soils. The washing mechanism could include the acid dissolution, ion exchange and complexation reaction between zinc ions and functional groups such as hydroxyl, carboxyl, amine and amide groups. This study provided the theoretical support for the exploitation and application of suitable washing agents used for the remediation of contaminated soils by heavy metals.


Assuntos
Quitosana , Recuperação e Remediação Ambiental , Metais Pesados , Poluentes do Solo , Ácido Cítrico , Humanos , Metais Pesados/análise , Solo , Poluentes do Solo/análise , Água , Zinco
13.
Lasers Med Sci ; 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34480664

RESUMO

It had been suggested, after facilitating the hatching process, improved pregnancy outcomes could be attained in embryos with thick and hard zona. This study aimed to determine the effect of zona thinning on pregnancy outcomes in poor-quality frozen-thawed blastocysts. This retrospective study included 230 women (≤ 40 years) who underwent frozen embryo transfer of poor-quality blastocysts (scored < 3BB). In total, 105 patients were in the assisted hatching group in which the zona was thinned by laser before transfer and 125 patients were in the control group in which the blastocysts were non-manipulated. Patients' demographics, cycle characteristics, and pregnancy outcomes were compared between the assisted hatching group and the control group. Further, regression analysis was applied to test the correlation between assisted hatching and live birth. All parameters in the patients' demographic characteristics and the cycle's characteristics were not significantly different between two groups. As for pregnancy outcomes, the second trimester pregnancy loss was significantly higher in the assisted hatching group (P = 0.035). Other pregnancy outcomes, including implantation rate, clinical pregnancy rate, biochemical miscarriage rate, the first trimester pregnancy loss, ongoing pregnancy rate, and live birth rate were comparable between two groups. The logistic regression analysis demonstrated no association between live birth and assisted hatching (univariate, OR = 0.787, P > 0.05; multivariate, OR = 0.652, P > 0.05), and the area under the receiver operating characteristic curve of the regression model was almost 0.7. It suggested that zona thinning may not be supposed to perform on poor-quality, frozen-thawed blastocysts. The indications of assisted hatching were still needed to further investigate.

14.
Sci Rep ; 11(1): 17603, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34475468

RESUMO

Over the past 50 years, temperatures on the Qinghai-Tibet Plateau (QTP) have risen roughly twice as fast as the global average, making it the most unpredictable region of environmental change due to global warming. In this paper, an Environmental Area Index model was developed using data from the Coupled Model Intercomparison Project to assess the ecological risk faced by QTP ecosystems under the influence of climate factors. The results show that ecological risk gradually decreases from northwest to the southeast, and there are different trends in ecological risk for each class in areas with different elevation gradients. As elevation increased, the proportion of potential risk areas gradually decreased, and the proportion of high- and higher-risk areas gradually increased. We predict that in the period 2021-2100, the overall ecological risk change trend on the QTP will not be obvious, but there will be a more obvious change on the vertical gradient. In general, under the existing global climate change scenario, the ecological risk faced by the QTP show a decreasing trend under the influence of climate factors, and the decrease in ecological risks is much higher at higher elevations than at lower elevations.

15.
Microb Pathog ; : 105170, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34492305

RESUMO

Bluetongue is a viral disease transmitted by the bite of bloodsucking insects, which mainly occurs in sheep, goats, and cattle. Bluetongue is characterized by fever, leukopenia, and severe catarrhal inflammation of the oral and gastrointestinal mucosa. The present study aimed to evaluate and analyze the prevalence of bluetongue and its associated risk factors in sheep and goats in China. We collected 59 publications from 1988 to 2019 through searches at ScienceDirect, PubMed, the Chongqing VIP Chinese journal database, Wanfang database, and Chinese Web of knowledge. In these studies, a total of 123,982 sheep and goats across 7 regions of China were investigated, and the pooled prevalence of bluetongue in sheep and goats was 18.6%, as assessed using serological methods. The prevalence of bluetongue in Southern China was 30.3%, which was significantly higher than that in Northeastern China (4.7%). The prevalence of bluetongue between sheep (12.9%) and goats (28.1%) was significantly different (P < 0.05). Detection methods subgroup analysis showed that the prevalence of bluetongue was significantly higher (P < 0.05) in the others group (43.8%) than in the agar immunodiffusion (15.9%) and enzyme-linked immunosorbent assay groups (20.5%). In addition, different geographical factors (latitude range, longitude range, altitude range, average precipitation, and average temperature) could affect the prevalence. Our results suggested that bluetongue is widespread in sheep and goats, and sheep and goats in contact with insect media, such as Culicoides, or in a warm and humid environment, could have an increased prevalence of bluetongue disease. Animal disease prevention and control departments should focus on continuous monitoring of the bluetongue epidemic in sheep and goats to prevent and control outbreaks.

16.
J Med Internet Res ; 23(9): e21974, 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34499042

RESUMO

BACKGROUND: Consumer health informatics (CHI) originated in the 1990s. With the rapid development of computer and information technology for health decision making, an increasing number of consumers have obtained health-related information through the internet, and CHI has also attracted the attention of an increasing number of scholars. OBJECTIVE: The aim of this study was to analyze the research themes and evolution characteristics of different study periods and to discuss the dynamic evolution path and research theme rules in a time-series framework from the perspective of a strategy map and a data flow in CHI. METHODS: The Web of Science core collection database of the Institute for Scientific Information was used as the data source to retrieve relevant articles in the field of CHI. SciMAT was used to preprocess the literature data and construct the overlapping map, evolution map, strategic diagram, and cluster network characterized by keywords. Besides, a bibliometric analysis of the general characteristics, the evolutionary characteristics of the theme, and the evolutionary path of the theme was conducted. RESULTS: A total of 986 articles were obtained after the retrieval, and 931 articles met the document-type requirement. In the past 21 years, the number of articles increased every year, with a remarkable growth after 2015. The research content in 4 different study periods formed the following 38 themes: patient education, medicine, needs, and bibliographic database in the 1999-2003 study period; world wide web, patient education, eHealth, patients, medication, terminology, behavior, technology, and disease in the 2004-2008 study period; websites, information seeking, physicians, attitudes, technology, risk, food labeling, patient, strategies, patient education, and eHealth in the 2009-2014 study period; and electronic medical records, health information seeking, attitudes, health communication, breast cancer, health literacy, technology, natural language processing, user-centered design, pharmacy, academic libraries, costs, internet utilization, and online health information in the 2015-2019 study period. Besides, these themes formed 10 evolution paths in 3 research directions: patient education and intervention, consumer demand attitude and behavior, and internet information technology application. CONCLUSIONS: Averaging 93 publications every year since 2015, CHI research is in a rapid growth period. The research themes mainly focus on patient education, health information needs, health information search behavior, health behavior intervention, health literacy, health information technology, eHealth, and other aspects. Patient education and intervention research, consumer demand, attitude, and behavior research comprise the main theme evolution path, whose evolution process has been relatively stable. This evolution path will continue to become the research hotspot in this field. Research on the internet and information technology application is a secondary theme evolution path with development potential.

17.
Artigo em Inglês | MEDLINE | ID: mdl-34505405

RESUMO

Human mesenchymal stem cells (hMSCs) are effective in treating disorders resulting from an inflammatory or heightened immune response. The hMSCs derived from induced pluripotent stem cells (ihMSCs) share the characteristics of tissue derived hMSCs but lack challenges associated with limited tissue sources and donor variation. To meet the expected future demand for ihMSCs, there is a need to develop scalable methods for their production at clinical yields while retaining immunomodulatory efficacy. Herein, we describe a platform for the scalable expansion and rapid harvest of ihMSCs with robust immunomodulatory activity using degradable gelatin methacryloyl (GelMA) microcarriers. GelMA microcarriers were rapidly and reproducibly fabricated using a custom microfluidic step emulsification device at relatively low cost. Using vertical wheel bioreactors, 8.8 to 16.3-fold expansion of ihMSCs was achieved over 8 days. Complete recovery by 5-minute digestion of the microcarriers with standard cell dissociation reagents resulted in >95% viability. The ihMSCs matched or exceeded immunomodulatory potential in vitro when compared with ihMSCs expanded on monolayers. This is the first description of a robust, scalable, and cost-effective method for generation of immunomodulatory ihMSCs, representing a significant contribution to their translational potential.

18.
Bioorg Med Chem ; 47: 116350, 2021 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-34536651

RESUMO

The antiapoptotic protein B-cell lymphoma 2 (Bcl-2), overexpressed in many tumor cells, is an attractive target for potential small molecule anticancer drug discovery. Herein, a series of novel derivatives with acyl sulfonamide skeleton was designed, synthesized, and evaluated as Bcl-2 inhibitors by means of bioisosteric replacement. Among them, compound 24g demonstrated equal efficient inhibition activity against RS4;11 cell line compared to positive control ABT-199. Moreover, it showed improved selectivity for Bcl-2/Bcl-xL inhibitory effects, the result of which was consistent with platelet toxicity studies. In vitro and in vivo pharmacokinetic properties of compound 24g had a significantly improved profiles. Taken together, those results suggested it as a promising candidate for development of novel therapeutics targeting Bcl-2 in cancer.

19.
Artigo em Inglês | MEDLINE | ID: mdl-34476560

RESUMO

PURPOSE: The present study aimed to examine the factors related to the morphological characteristics of the femoral condyle in posterior cruciate ligament rupture in female and male populations. METHODS: One hundred and three patients (41 females, 62 males) with posterior cruciate ligament rupture from 2010 to 2020 were included in this retrospective case-control study. The sex and age of the posterior cruciate ligament rupture group were matched to those of the control group (41 females, 62 males; age range 16-69 years). Magnetic resonance imaging was used to measure the intercondylar notch width, femoral condylar width, and intercondylar notch angle in both the axial and coronal images. The 'α' angle was also measured using magnetic resonance imaging. The notch width index is the ratio of the intercondylar notch width to the femoral condylar width. Three types of intercondylar notch shapes (types A, U, and W) were evaluated in the axial magnetic resonance imaging images. RESULTS: The difference in the mean coronal notch width index between the study groups was statistically significant in the female population. The difference in the mean coronal femoral condylar width between the study groups was statistically significant in the male population. CONCLUSIONS: A larger coronal notch width index was the greatest risk factor for posterior cruciate ligament rupture in the female population. In the male population, decreased coronal condylar width was the greatest risk factor for posterior cruciate ligament rupture. The results did not indicate that patients with a PCL rupture have a stenotic intercondylar notch. Posterior cruciate ligament injury prevention strategies could be applied to females with a larger coronal notch width index and males with a decreased condylar width. LEVELS OF EVIDENCE: Level III.

20.
Artigo em Inglês | MEDLINE | ID: mdl-34476693

RESUMO

Rising human population has increased the utilization of available resources for food, clothes, medicine, and living space, thus menacing natural environment and mounting the gap between available resources, and the skills to meet human desires is necessary. Humans are satisfying their desires by depleting available natural resources. Therefore, multifunctional plants can contribute towards the livelihoods of people, to execute their life requirements without degrading natural resources. Thus, research on multipurpose industrial crops should be of high interest among scientists. Hemp, or industrial hemp, is gaining research interest because of its fastest growth and utilization in commercial products including textile, paper, medicine, food, animal feed, paint, biofuel, biodegradable plastic, and construction material. High biomass production and ability to grow under versatile conditions make hemp, a good candidate species for remediation of polluted soils also. Present review highlights the morphology, adaptability, nutritional constituents, textile use, and medicinal significance of industrial hemp. Moreover, its usage in environmental conservation, building material, and biofuel production has also been discussed.

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