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1.
J Colloid Interface Sci ; 638: 109-122, 2023 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-36736113

RESUMO

Metal oxide-oxide interface on supported catalyst has been rarely studied due to the complex interfacial structure and synthetic challenge. Herein, different Ag-supported CeO2/Co3O4 samples with various covered-state of CeO2 were prepared for catalytic soot oxidation. In comparison, catalytic activity was significantly improved by grafting CeO2 on Co3O4, in which the best performing Ag/CoCe-2 exhibited remarkable catalytic performance towards soot oxidation with a T50 of 290.5 â„ƒ under 10 % O2/N2. Catalyst characterization investigated by Scanning Electron Microscope (SEM), quasi in-situ X-ray Photoelectron Spectroscopy (XPS), in-situ Raman, etc. revealed that this outstanding promotion in catalytic activity can be principally ascribed to the formation of the CeO2/Co3O4 interface. An appropriate CeO2 dosage maximized the contact and interaction between Co3O4 and CeO2, resulting in the largest CeO2/Co3O4 interface featured with abundant generated superoxide species and activated surface lattice oxygen. Density functional theory (DFT) calculations were also carried out for the oxygen vacancy formation energy, Gibbs free energy, etc. In presence of the CeO2/Co3O4 interface, a charge density redistribution around the adsorbed reactants at oxygen vacancies could be formed, owing to the efficient charge transfer enhanced by the electron-appealing effect. The change in electronic structure favored reducing the oxygen vacancy formation energy and boosting the lattice oxygen activation induced by the hybridized Co-O-Ce bonds, finally lowering the adsorption and activation barriers for reactive species and accelerating the reaction kinetics.

4.
BMC Pulm Med ; 23(1): 37, 2023 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-36703125

RESUMO

BACKGROUND: Donor-derived cell-free DNA (dd-cfDNA) has been applied to monitor acute rejection (AR) in kidney and heart transplantation. This study was aimed to investigate the application of dd-cfDNA levels in the diagnosis of AR and chronic lung allograft dysfunction (CLAD) among the lung transplantation recipients (LTRs). METHODS: One hundred and seventy LTRs were enrolled at the First Affiliated Hospital of Guangzhou Medical University between 1 June 2015 and 30 March 2021. Patients were divided into 4 groups: stable group, AR group, infection group and CLAD group. The level of dd-cfDNA was analyzed using target region sequencing and the performance characteristics of dd-cfDNA for diagnosis of AR and CLAD were determined, respectively. RESULTS: Kruskal-Wallis test showed that there were some significant differences in the level of dd-cfDNA (%) among the 4 groups, with p < 0.001. Among them, the level of dd-cfDNA (%) was highest (median 2.17, IQR [1.40-3.82]) in AR group, and higher in CLAD group (median 1.07, IQR [0.98-1.31]), but lower in infection group (median 0.71, IQR [0.57-1.07]) and lowest in stable group (median 0.71, IQR [0.61-0.84]). AUC-ROC curve analysis showed that the threshold of dd-cfDNA for AR was 1.17%, with sensitivity being 89.19% and specificity being 86.47%, and the optimal threshold of 0.89% was determined of CLAD, with sensitivity being 95.00% and specificity of 76.99%. CONCLUSIONS: Plasma dd-cfDNA could be a useful tool for the assessment of lung allograft rejection, including AR and CLAD, and holds promise as a noninvasive biomarker for "allograft injury" in both acute and chronic rejection following lung transplantation.


Assuntos
Ácidos Nucleicos Livres , Transplantados , Humanos , Rejeição de Enxerto/diagnóstico , Pulmão , Aloenxertos
6.
J Exp Bot ; 2023 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-36638269

RESUMO

Sporopollenin polymer is a major component of pollen exine. Fatty acid derivatives synthesized in tapetum are among the precursors of sporopollenin. Progresses have been made to understand sporopollenin metabolism in rice, however, the underlying molecular mechanisms remain elusive. We found that OsTKPR2 and OsTKPR1 share similar expression pattern, and their coding proteins have similar subcellular localization and enzyme activities towards reduce tetraketide α-pyrone and hydroxylated tetraketide α-pyrone. Unexpectedly, OsTKPR1pro:OsTKPR2-eGFP could not rescue the phenotype of ostkpr1-4. Three independent ostkpr2 mutant lines generated by CRISPR/Cas9 displayed reduced male fertility to various extents which were correlated with the severity of gene disruptions. Notably, anther cuticle, Ubisch bodies, and pollen development were affected in ostkpr2-1 mutant, where thinner pollen exine was noticed. OsTKPR1 and OsTKPR2 were integrated into a metabolon including OsACOS12 and OsPKS2, which resulted in a significant increased enzymatic efficiency when both OsTKPR1 and OsTKPR2 were present, indicating the mutual dependence of OsTKPR2 and OsTKPR1 for their full biochemical activities. Thus, our results demonstrated that OsTKPR2 is required for anther and pollen development where an OsTKPR2-containing metabolon is functional during rice sporopollenin synthesis. Furthermore, the cooperation and possible functional divergence between OsTKPR2 and OsTKPR1 is also discussed.

7.
Artigo em Inglês | MEDLINE | ID: mdl-36702768

RESUMO

OBJECTIVE: It remains controversial on whether the choice of the daily eating window early or later in TRE intervention (early or later TRE) has different effects on weight loss and metabolic health. A network meta-analysis was performed to evaluate the efficacy between early and later TRE in adults with obesity or overweight. METHODS: We searched PubMed, Embase, Web of Science, and Cochrane Library for randomized controlled trials (RCTs) published until Oct 16, 2022. We conducted a network meta-analysis to evaluate the efficacy of early and later TRE on body weight and metabolic parameters, including glycemic metabolism, blood pressure, and lipid profiles. RESULTS: 12 RCTs with 730 obese or overweight adults were included in this meta-analysis. Both early TRE and later TRE elicited moderate reductions in body weight and insulin resistance (HOMA-IR) when compared to non-TRE. Interestingly, early TRE showed more effectiveness than later TRE in improving insulin resistance (early vs. later TRE: -0.44, 95% CI, -0.86 to -0.02; P < 0.05), whereas no significant difference was detected in weight loss (early vs. later TRE: -0.31 kg; 95% CI, -1.15 to 0.53 kg; P >0 .05). In addition, early TRE rather than later TRE showed significant benefits in glycemic metabolism and blood pressure when compared to non-TRE. No significant differences between early and later TRE were observed for fasting blood glucose, blood pressure, and lipid profiles. CONCLUSIONS: This meta-analysis suggests that people may choose early TRE for more effective weight management and metabolic benefits. Nevertheless, further large-scale RCTs are warranted to verify our findings.

8.
Magn Reson Med ; 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36705076

RESUMO

PURPOSE: This work aims to develop a novel distortion-free 3D-EPI acquisition and image reconstruction technique for fast and robust, high-resolution, whole-brain imaging as well as quantitative T 2 * $$ {\mathrm{T}}_2^{\ast } $$ mapping. METHODS: 3D Blip-up and -down acquisition (3D-BUDA) sequence is designed for both single- and multi-echo 3D gradient recalled echo (GRE)-EPI imaging using multiple shots with blip-up and -down readouts to encode B0 field map information. Complementary k-space coverage is achieved using controlled aliasing in parallel imaging (CAIPI) sampling across the shots. For image reconstruction, an iterative hard-thresholding algorithm is employed to minimize the cost function that combines field map information informed parallel imaging with the structured low-rank constraint for multi-shot 3D-BUDA data. Extending 3D-BUDA to multi-echo imaging permits T 2 * $$ {\mathrm{T}}_2^{\ast } $$ mapping. For this, we propose constructing a joint Hankel matrix along both echo and shot dimensions to improve the reconstruction. RESULTS: Experimental results on in vivo multi-echo data demonstrate that, by performing joint reconstruction along with both echo and shot dimensions, reconstruction accuracy is improved compared to standard 3D-BUDA reconstruction. CAIPI sampling is further shown to enhance image quality. For T 2 * $$ {\mathrm{T}}_2^{\ast } $$ mapping, parameter values from 3D-Joint-CAIPI-BUDA and reference multi-echo GRE are within limits of agreement as quantified by Bland-Altman analysis. CONCLUSIONS: The proposed technique enables rapid 3D distortion-free high-resolution imaging and T 2 * $$ {\mathrm{T}}_2^{\ast } $$ mapping. Specifically, 3D-BUDA enables 1-mm isotropic whole-brain imaging in 22 s at 3T and 9 s on a 7T scanner. The combination of multi-echo 3D-BUDA with CAIPI acquisition and joint reconstruction enables distortion-free whole-brain T 2 * $$ {\mathrm{T}}_2^{\ast } $$ mapping in 47 s at 1.1 × 1.1 × 1.0 mm3 resolution.

9.
Sci Total Environ ; 866: 161421, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36621491

RESUMO

Understanding the spatial variability of soil organic matter (SOM), soil total nitrogen (STN), soil total phosphorus (STP), and soil total potassium (STK) is important to support site-specific agronomic management, food production, and climate change adaptation. High-resolution remote sensing imageries have emerged as an innovative solution to investigate the spatial variation in agricultural soils with machine learning (ML) algorithms. However, the predictive power of the individual and combined effects of Sentinel-1 (S1) synthetic aperture radar (SAR) and Sentinel-2 (S2) multispectral images for mapping soil properties, especially STN, STP, and STK, have rarely been investigated. Moreover, single ML model may achieve unstable performance for predicting multiple soil properties due to strong spatial heterogeneity. This study explored the combine use of S1, S2, and DEM derivatives to map SOM, STN, STP, and STK content of a sloped cropland of northeastern China. A two-step method with a weighted sum of four ML models was proposed to improve the accuracy and robustness in predicting multiple soil properties. Our results showed that single ML model has various performance in predicting the four soil properties. The optimal ML models could explain approximately 56 %, 53 %, 56 % and 37 % of the variability of SOM, STN, STP, and STK, respectively. Using the weights estimated through a 10-fold cross-validation procedure, the two-step ensemble learning model was retrained and showed more robust performance than the four ML models, in which the prediction accuracy was improved by 2.38 %, 1.40 %, 3.52 %, and 3.29 % for SOM, STN, STP, and STK, respectively. Our results also showed that the optical S2 derived features, especially the two S2 short-wave infrared bands, enhanced vegetation index, and soil adjusted vegetation index, were more important for soil property prediction than S1 data and DEM derivatives. Compared with individual sensor, a combination of S1 and S2 data yielded more accurate predictions of STN and STP but not for SOM and STK. The results of this study highlight the potential of high-resolution S1 and S2 data and the two-step method for soil property prediction at farmland scale.

10.
Artigo em Inglês | MEDLINE | ID: mdl-36627498

RESUMO

PURPOSE: Human post mortem studies have described the topographical patterns of tau pathology in progressive supranuclear palsy (PSP). Recent advances in tau PET tracers are expected to herald the next era of PSP investigation for early detection of tau pathology in living brains. This study aimed to investigate whether 18F-Florzolotau PET imaging may capture the distribution patterns and regional vulnerability of tau pathology in PSP, and to devise a novel image-based staging system. METHODS: The study cohort consisted of 148 consecutive patients with PSP who had undergone 18F-Florzolotau PET imaging. The PSP rating scale (PSPrs) was used to measure disease severity. Similarities and differences of tau deposition among different clinical phenotypes were examined at the regional and voxel levels. An 18F-Florzolotau pathological staging system was devised according to the scheme originally developed for post mortem data. In light of conditional probabilities for the sequence of events, an 18F-Florzolotau modified staging system by integrating clusters at the regional level was further developed. The ability of 18F-Florzolotau staging systems to reflect disease severity in terms of PSPrs score was assessed by analysis of variance. RESULTS: The distribution patterns of 18F-Florzolotau accumulation in living brains of PSP showed a remarkable similarity to those reported in post mortem studies, with the binding intensity being markedly higher in Richardson's syndrome. Moreover, 18F-Florzolotau PET imaging allowed detecting regional vulnerability and tracking tau accumulation in an earlier fashion compared with post mortem immunostaining. The 18F-Florzolotau staging systems were positively correlated with clinical severity as reflected by PSPrs scores. CONCLUSIONS: 18F-Florzolotau PET imaging can effectively capture the distribution patterns and regional vulnerability of tau pathology in PSP. The 18F-Florzolotau modified staging system holds promise for early tracking of tau deposition in living brains.

11.
Small Methods ; : e2201467, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36631288

RESUMO

Post-treatment is an essential passivation step for the state-of-the-art perovskite solar cells (PSCs) but the additional role is not yet exploited. In this work, perovskite film is fabricated under ambient air with wide humidity window and identify that chloride redistribution induced by post-treatment plays an important role in high performance. The chlorine/iodine ratio on the perovskite surface increases from 0.037 to 0.439 after cyclohexylmethylammonium iodide (CHMAI) treatment and the PSCs deliver a champion power conversion efficiency (PCE) of 24.42% (certificated 23.60%). The maximum external quantum efficiency of electroluminescence (EQEEL ) reaches to 10.84% with a radiance of 170 W sr-1  m-2 , forming the reciprocity relation between EQEEL and nonradiative open-circuit voltage loss (86.0 mV). After thermal annealing, 2D component of perovskite will increase while chloride decline, leading to improved photovoltage but reduced fill factor. Hence, it distinguishes that chloride enrichment can improve charge transport/recombination simultaneously and 2D passivation can suppress the nonradiative recombination. Moreover, CHMAI can leverage their roles in charge transport/recombination for better performance than phenylethylammonium iodide (Cl/I = 0.114, PCE = 23.32%), due to the stronger binding energy of Cl- . This work provides the insight that the chloride fixation can improve the photovoltaic performance.

12.
J Coll Physicians Surg Pak ; 33(1): 47-52, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36597235

RESUMO

OBJECTIVE: To investigate the virological relapse (VR) rate and prognostic value of the HBsAg level at treatment completion for predicting sustained off-treatment response in HBeAg-negative patients after neucleos(t)ide analog (NA) therapy. STUDY DESIGN: Prospective observational cohort study. PLACE AND DURATION OF STUDY: The Second Hospital of Shandong University, Ji'nan, China, between December 2001 and January 2020. METHODOLOGY: Eighty-one HBeAg-negative chronic hepatitis B patients who stopped NA treatment were included. Factors associated with the VR were identified using univariate and multivariate Cox regression models. RESULTS: Of the 81 patients, 42 had sustained off-treatment response with a median follow-up of 60.0 months (interquartile range [IQR] 33.0-111.0 months). Thirty-nine patients relapsed and 32 relapsed within the first year. The cumulative VR rates were 34.6%, 41.0%, 42.5%, 48.1%, and 55.8% at 1, 2, 3, 5, and 10 years off-therapy, respectively. For patients with end-of-treatment (EOT) HBsAg <250 IU/mL, the 10-year cumulative VR rate was 26.0%. Time to HBV DNA negativity (median, 2 months [IQR 1.0-3.0 months]) and age at EOT were also independent predictors of sustained off-treatment response. CONCLUSION: Discontinuing long-term NA treatment is a feasible option for HBeAg-negative chronic hepatitis B patients whose HBsAg levels are low, and HBsAg <250 IU/mL may be an acceptable cut-off value. Younger age at EOT and shorter time to HBV DNA negativity are also independent factors associated with sustained off-treatment response. KEY WORDS: Hepatitis B surface antigen, Nucleos(t)ide analogs, Cessation, Relapse.


Assuntos
Antígenos de Superfície da Hepatite B , Hepatite B Crônica , Humanos , Hepatite B Crônica/tratamento farmacológico , Antígenos E da Hepatite B/uso terapêutico , Estudos Prospectivos , DNA Viral , Antivirais/uso terapêutico , Recidiva , Vírus da Hepatite B/genética , Resultado do Tratamento
13.
Neurol Res ; : 1-8, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36621968

RESUMO

BACKGROUND: Cerebral microbleeds (CMBs) are subclinical lesions of the brain parenchyma and an important marker for the clinical diagnosis of central nervous system vascular disease. However, the relationship between CMBs and cerebral infarction, cerebral hemorrhage, and cognitive impairment remains unclear. METHODS: In order to explore the cognitive function and risk factors of patients with acute cerebral infarction (ACI) complicated with cerebral microbleeds, 190 patients with ACI were collected. The patients were divided into groups with CMBs (n = 108) and groups without CMBs (n = 82) according to the presence or absence of CMBs. The general data, various examination indicators, Montreal Cognitive Assessment Scale (MoCA) scores of the two groups of patients were analyzed. Sixty healthy controls who underwent physical examination in our hospital during the same period were included as the healthy control group. RESULTS: ACI patients with CMBs had significantly higher rates of leukoaraiosis, hyperhomocysteinemia, hypercholesterolemia, and hypertension. Cognitive function was significantly lower in ACI patients with CMBs. Serum D-dimer, serum high-sensitivity C-reactive protein, serum neuron-specific enolase, and serum S100ß of ACI patients with CMBs were all negatively correlated with their MoCA scores. CONCLUSION: ACI patients with CMBs tended to have lower cognitive abilities than ACI patients without CMBs.

14.
Zhongguo Gu Shang ; 36(1): 72-8, 2023 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-36653011

RESUMO

OBJECTIVE: To provide basic data for clinical application and individualized design of lumbar disc prostheses by measuring the anatomical parameters of lumbar intervertebral discs and endplates in healthy adults with CT three-dimensional reconstruction technology. METHODS: A retrospective analysis was performed on 200 males and 200 females with normal lumbar spine who were admitted to the imaging center or outpatient department of the Second Affiliated Hospital of Xinjiang Medical University from September 2019 to December 2020. The age ranged from 20 to 60 years old, with an average of (40.61±11.22) years old. The measurement segment was L1-S1 intervertebral disc, and the measurement indicators included the axial anteroposterior diameter and transverse diameter of the intervertebral disc, sagittal anterior, middle and posterior height, coronal left and right height, intervertebral space angle, and transverse and anteroposterior diameters of the upper and lower endplates of each vertebral body. RESULTS: ①In terms of gender, the anatomical parameters of L1-S1 disc axial diameter, transverse diameter, sagittal anterior, middle and posterior height, left and right coronal height and intervertebral space angle were all higher in males than in females(P<0.05), and the anatomical parameters of upper and lower endplates of L1-S1 vertebral body were higher in males than in females(P<0.001). ②In comparison of sagittal height of anterior, middle and posterior intervertebral discs, the sagittal height of L1-L5 intervertebral discs was middle-high > anterior-high > posterior-high(P<0.001), while that of L5S1 intervertebral disc was anterior-high > middle-high > posterior-high (P<0.001). ③In the comparison of left and right coronal height, there was no statistical significance in the left and right coronal height of L1-S1 disc between male and female(P>0.05). ④The L1-S1 intervertebral spaces angle between male and female increased with the increase of vertebral body segments. ⑤The anterior and posterior diameters and transverse diameters of upper and lower of L1-S1 vertebral bodies endplates were height in males than in females(P<0.001). CONCLUSION: The results suggest that gender differences should be considered in the design of adult lumbar disc prostheses. The anatomical parameters of the lumbar intervertebral disc varied with the increase of the vertebral body sequence, suggesting that different anatomical parameters of the intervertebral disc should be considered in the design of the artificial intervertebral disc, and the changes in the height of the sagittal position suggest that the design of the intervertebral disc should be wedge-shaped.


Assuntos
Disco Intervertebral , Adulto , Humanos , Masculino , Feminino , Adulto Jovem , Pessoa de Meia-Idade , Estudos Retrospectivos , Disco Intervertebral/diagnóstico por imagem , Vértebras Lombares/diagnóstico por imagem , Região Lombossacral , Tomografia Computadorizada por Raios X
15.
Cell Death Differ ; 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36681781

RESUMO

cGAS/DncV-like nucleotidyltransferase (CD-NTase) family members are immune sensors that synthesize diverse nucleotide signals to initiate antiviral response in bacteria and animals. As a founding member of CD-NTase enzyme, cGAS has been identified as a key sensor for cytoplasmic DNA and type I interferons (IFNs) signaling in metazoan. However, the functions of other metazoan CD-NTases remain enigmatic. Here, we showed that Mab-21 domain-containing protein 2 (MB21D2), another member of the CD-NTase family, plays a positive role in modulating the cGAS-STING signaling in myeloid cells. Deficiency of MB21D2 in THP-1 cells or mice macrophages led to impaired production of type I interferon upon DNA stimulation. Consistently, Mb21d2-/- mice showed more susceptible to infection with DNA virus and faster growth of melanoma, compared to its counterparts. Mechanistically, MB21D2 specially bound with the N-terminal of cGAS, facilitated its liquid phase condensation and DNA-binding activity, leading to the enhanced production of cGAMP and subsequent IFN-ß production. Thus, our findings unveiled that the CD-NTase family member MB21D2 contributes to host antiviral and antitumor responses by enhancing cGAS activation.

16.
Artigo em Inglês | MEDLINE | ID: mdl-36696895

RESUMO

BACKGROUND: Skeletal muscle atrophy is a common condition without a pharmacologic therapy. AGGF1 encodes an angiogenic factor that regulates cell differentiation, proliferation, migration, apoptosis, autophagy and endoplasmic reticulum stress, promotes vasculogenesis and angiogenesis and successfully treats cardiovascular diseases. Here, we report the important role of AGGF1 in the pathogenesis of skeletal muscle atrophy and attenuation of muscle atrophy by AGGF1. METHODS: In vivo studies were carried out in impaired leg muscles from patients with lumbar disc herniation, two mouse models for skeletal muscle atrophy (denervation and cancer cachexia) and heterozygous Aggf1+/- mice. Mouse muscle atrophy phenotypes were characterized by body weight and myotube cross-sectional areas (CSA) using H&E staining and immunostaining for dystrophin. Molecular mechanistic studies include co-immunoprecipitation (Co-IP), western blotting, quantitative real-time PCR analysis and immunostaining analysis. RESULTS: Heterozygous Aggf1+/- mice showed exacerbated phenotypes of reduced muscle mass, myotube CSA, MyHC (myosin heavy chain) and α-actin, increased inflammation (macrophage infiltration), apoptosis and fibrosis after denervation and cachexia. Intramuscular and intraperitoneal injection of recombinant AGGF1 protein attenuates atrophy phenotypes in mice with denervation (gastrocnemius weight 81.3 ± 5.7 mg vs. 67.3 ± 5.1 mg for AGGF1 vs. buffer; P < 0.05) and cachexia (133.7 ± 4.7 vs. 124.3 ± 3.2; P < 0.05). AGGF1 expression undergoes remodelling and is up-regulated in gastrocnemius and soleus muscles from atrophy mice and impaired leg muscles from patients with lumbar disc herniation by 50-60% (P < 0.01). Mechanistically, AGGF1 interacts with TWEAK (tumour necrosis factor-like weak inducer of apoptosis), which reduces interaction between TWEAK and its receptor Fn14 (fibroblast growth factor-inducing protein 14). This leads to inhibition of Fn14-induced NF-kappa B (NF-κB) p65 phosphorylation, which reduces expression of muscle-specific E3 ubiquitin ligase MuRF1 (muscle RING finger 1), resulting in increased MyHC and α-actin and partial reversal of atrophy phenotypes. Autophagy is reduced in Aggf1+/- mice due to inhibition of JNK (c-Jun N-terminal kinase) activation in denervated and cachectic muscles, and AGGF1 treatment enhances autophagy in two atrophy models by activating JNK. In impaired leg muscles of patients with lumbar disc herniation, MuRF1 is up-regulated and MyHC and α-actin are down-regulated; these effects are reversed by AGGF1 by 50% (P < 0.01). CONCLUSIONS: These results indicate that AGGF1 is a novel regulator for the pathogenesis of skeletal muscle atrophy and attenuates skeletal muscle atrophy by promoting autophagy and inhibiting MuRF1 expression through a molecular signalling pathway of AGGF1-TWEAK/Fn14-NF-κB. More importantly, the results indicate that AGGF1 protein therapy may be a novel approach to treat patients with skeletal muscle atrophy.

17.
Cell Mol Biol Lett ; 28(1): 4, 2023 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-36658472

RESUMO

BACKGROUND: Ischemia-reperfusion injury (IRI) is an inevitable process in renal transplantation that significantly increases the risk of delayed graft function, acute rejection, and even graft loss. Formyl peptide receptor 2 (FPR2) is an important receptor in multiple septic and aseptic injuries, but its functions in kidney IRI are still unclear. This study was designed to reveal the pathological role of FPR2 in kidney IRI and its functional mechanisms. METHODS: To explore the mechanism of FPR2 in kidney IRI, the model rats were sacrificed after IRI surgery. Immunofluorescence, enzyme-linked immunosorbent assays, and western blotting were used to detect differences in the expression of FPR2 and its ligands between the IRI and control groups. WRW4 (WRWWWW-NH2), a specific antagonist of FPR2, was administered to kidney IRI rats. Kidney function and pathological damage were detected to assess kidney injury and recovery. Flow cytometry was used to quantitatively compare neutrophil infiltration among the experimental groups. Mitochondrial formyl peptides (mtFPs) were synthesized and administered to primary rat neutrophils together with the specific FPR family antagonist WRW4 to verify our hypothesis in vitro. Western blotting and cell function assays were used to examine the functions and signaling pathways that FPR2 mediates in neutrophils. RESULTS: FPR2 was activated mainly by mtFPs during the acute phase of IRI, mediating neutrophil migration and reactive oxygen species production in the rat kidney through the ERK1/2 pathway. FPR2 blockade in the early phase protected rat kidneys from IRI. CONCLUSIONS: mtFPs activated FPR2 during the acute phase of IRI and mediated rat kidney injury by activating the migration and reactive oxygen species generation of neutrophils through the ERK1/2 pathway.


Assuntos
Neutrófilos , Receptores de Formil Peptídeo , Traumatismo por Reperfusão , Animais , Ratos , Sistema de Sinalização das MAP Quinases , Neutrófilos/metabolismo , Peptídeos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptores de Formil Peptídeo/metabolismo , Traumatismo por Reperfusão/metabolismo
18.
Artigo em Inglês | MEDLINE | ID: mdl-36661875

RESUMO

Immediate implant placement and provisionalization with subepithelial connective tissue graft is considered the reference therapy for achieving a good esthetic outcome, especially in cases with a thin periodontal phenotype. Positioning sutures are usually required to maintain graft stability. This article describes the use of a modified suture technique involving vertical/horizontal double-parallel mattress sutures in immediate implant placement to achieve stable passive fixation of the graft in the appropriate position and to provide a more equal distribution of tension in the wound. A novel, simplified, reproducible technique is described in two cases of immediate implant placement and provisionalization with subepithelial connective tissue grafting and double-parallel mattress sutures.


Assuntos
Implantes Dentários para Um Único Dente , Implantes Dentários , Carga Imediata em Implante Dentário , Gengiva/transplante , Carga Imediata em Implante Dentário/métodos , Tecido Conjuntivo/transplante , Técnicas de Sutura , Resultado do Tratamento , Estética Dentária
19.
Int J Mol Sci ; 24(2)2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36675039

RESUMO

Colorectal cancer (CRC) is critically related to aging and severely threatens human lives. To better explore the effects of aging on CRC progression and therapy outcome, a reliable aging subtypes identification of CRC is urgently desired. Here, 28 aging-related genes associated with the CRC prognosis were selected by univariate Cox analyses. Based on these 28 genes, CRC patients were divided into the aging subtype and young subtype by non-negative matrix factorization clustering. Aging subtype and young subtype of CRC were identified with distinct molecular features and clinical prognosis. The aging subtype was characterized by upregulation of senescence-associated secretory phenotype, higher frequencies of TP53 and immune checkpoint molecules, and high sensitivity to protein kinase and angiogenesis inhibitors. Furthermore, 14 genes were selected by LASSO penalized Cox regression analyses for aging-related risk signature construction. The constructed aging risk signature exhibited good prediction and the nomogram showed robust discrimination power over the traditional CRC staging system. In conclusion, this study successfully established aging subtype and young subtype of CRC, which is helpful to identify patients with aging characteristics to evaluate prognosis and treatment outcomes. Introducing aging-based subtypes into clinical concern and patient prognostication provides new opportunities for personalized CRC treatment.


Assuntos
Neoplasias Colorretais , Imunoterapia , Humanos , Envelhecimento , Algoritmos , Inibidores da Angiogênese , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/genética , Neoplasias Colorretais/terapia
20.
Phytomedicine ; 110: 154635, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36587416

RESUMO

BACKGROUND: Fritillariae Bulbus (FB) is widely used as a traditional medicine for the treatment of lung meridian diseases. It has been proved that FB has good anti-non-small cell lung cancer (NSCLC) activity. However, the active components and potential mechanism are still not clear. PURPOSE: To reveal the bioactive components of FB against NSCLC and potential mechanism through spectrum-effect relationship and proteomics. METHOD: First, the FB extract was chemically profiled by UHPLC-QTOF-MS and the inhibitory effect of FB extract on A549 cell viability was evaluated by Cell Counting Kit-8 assay. Second, orthogonal-partial least squares-regression analysis was applied to screen potential active compounds through correlating the chemical profile with corresponding inhibitory effect. Third, the anti-NSCLC activities of potential active components were further investigated in terms of cell proliferation, cell cycle and cell apoptosis in vitro and tumor growth in vivo. Finally, proteomics was utilized to reveal the underlying anti-NSCLC mechanism. RESULTS: Six potential active components including verticine, verticinone, zhebeirine, ebeiedinone, yibeissine and peimisine were screened out by spectrum-effect relationship. Among them, zhebeirine showed higher inhibitory effect on A549 cell viability with IC50 value of 36.93 µM and dosage-dependent inhibition of A549 xenograft tumor growth in nude mice. Proteomics and western blotting assays indicated that zhebeirine could arrest cell cycle by down-regulating the expressions of CDK1, CDK2, Cyclin A2, Cyclin B2 and inhibiting the phosphorylation of p53. Moreover, the proteins participating in p53 signaling pathway including PCNA, 14-3-3σ, CHEK1 were significantly decreased, which suggested that zhebeirine affected cell cycle progression through p53 signaling pathway. CONCLUSION: This study not only provides scientific evidence to support the clinical application of FB against NSCLC, but also demonstrates that zhebeirine is a promising anti-NSCLC lead compound deserving further studies.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Pulmonares , Animais , Camundongos , Humanos , Neoplasias Pulmonares/patologia , Camundongos Nus , Proteína Supressora de Tumor p53/metabolismo , Proteômica , Carcinoma Pulmonar de Células não Pequenas/patologia , Proliferação de Células , Apoptose , Linhagem Celular Tumoral
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