Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.827
Filtrar
1.
Talanta ; 236: 122891, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34635270

RESUMO

A molecularly imprinted biosensor for lysozyme based on the polymer nanoparticles self-assembled from water-soluble and electroactive poly (γ-glutamic acid) modified with 3-aminothiophene copolymer were prepared. The water-soluble copolymer made imprinting of lysozyme in aqueous solution possible and thus facilitated improvement of the activity of LYS. Subsequent electro-polymerization not only locked the recognition site between copolymer and lysozyme but also created a conductive polymer network, which can enhance the electron transfer rate and increase the conductivity of the film. The prepared molecularly imprinted biosensor exhibited a wide linear range from 1 × 10-10 to 1 × 10-5 mg mL-1, and satisfactory selectivity, stability, repeatability for lysozyme detection.


Assuntos
Técnicas Biossensoriais , Impressão Molecular , Nanopartículas , Muramidase , Polímeros , Água
2.
Comput Human Behav ; 126: 107019, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34602727

RESUMO

Effectively engaging citizens during crises is critical for governments to disseminate timely information and help the public to adjust to the constantly changing conditions. In particular, promoting youth engagement not only enhances crisis awareness and resilience among the young generation, but also has a positive impact on youths' social participation and responsibility. With the increasing popularity of online video services, leveraging online videos to disseminate authoritative information has become a method widely adopted by government. To enhance youth awareness and engagement, two new video-based crisis communication strategies have been utilized on a popular youth-targeted video platform Bilibili in China: creating recreational videos such as animation and music videos, and collaborating with individual video-uploaders in video making. However, their impacts and results are largely unknown, which motivates our study. Guided by Entertainment Education (EE) and Collaborative Governance (CG), we report, to our best knowledge, the first systematic study on how recreational video category and government-citizen collaboration would influence youth engagement focusing on 3347 COVID-19-related government-generated videos on Bilibili. This study reveals that recreational videos successfully promote youth engagement including interaction, feedback and sharing. Collaboration with individual uploaders in video making also has a substantially positive impact on youth engagement. Through an in-depth qualitative content analysis of user-generated commentaries, we further unpacked the unique values (e.g., trust work for youth participation) as well as latent limitations (e.g., imbalanced topic distribution) of the two new strategies. We discuss how the findings enrich EE and CG theoretically, and provide practical implications to effective and engaging communication strategies during crises.

3.
Food Chem ; 368: 130865, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34428692

RESUMO

There are few studies on controlling the formation of heterocyclic amines (HAs) by adding polyphenols to roasted lamb. The aim of this study was to assess the inhibitory effect of 0.025, 0.125, and 0.625 mmol of chlorogenic acid, epicatechin, rutin, quercetin and quinic acid on the formation of HAs in charcoal roasted lamb, respectively, by UHPLC-MS/MS. The results indicated that Harman, Norharman, PhIP, IQx and 8-MeIQx were detected, but both chlorogenic acid and epicatechin greatly inhibited the formation of IQx, 8-MeIQx, Norharman, Harman and PhIP, and epicatechin had better inhibitory effect than chlorogenic acid. PLSR-VID analysis suggested the key precursors of HAs formation in roasted lamb were glucose, ribose, fructose, isoleucine, valine, and lysine. In addition, this work also implied that the potential mechanism of inhibition of HAs formation by chlorogenic acid and epicatechin in roasted lamb might attribute to competitive chemical reactions between polyphenols and key precursors of HAs.


Assuntos
Catequina , Compostos Heterocíclicos , Carne Vermelha , Aminas/análise , Animais , Carvão Vegetal , Ácido Clorogênico , Culinária , Compostos Heterocíclicos/análise , Carne/análise , Carne Vermelha/análise , Ovinos , Espectrometria de Massas em Tandem
4.
Environ Res ; 203: 111819, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34358504

RESUMO

Phenol's presence in aqueous solution due to the pollution from chemical and agricultural industries (e.g., coking tobacco leaves) causes severe environmental problems. As a result, many scientists and engineers search for catalysts to remove phenol from water by photodegradation. Thus, we synthesized Pt-doped TiO2-ZnO@ZIF-8 core@shell particles (Pt/TiO2-ZnO@ZIF-8) by a simple method involving crystallization, absorption, pyrolysis and growth steps. The resulting materials were analyzed by the powder X-ray diffraction (XRD), scanning and transmission electron microscopies (SEM and TEM, respectively), surface area measurements and UV-vis absorption spectroscopy. The photocatalytic activities of our materials were evaluated by phenol degradation in aqueous solutions. Pt-doped TiO2-ZnO particles possessed a polyhedral structure and exhibited broad absorption above 400 nm. Coating with ZIF-8 increased the specific surface area of the Pt-doped TiO2-ZnO particles. Both Pt doping and ZIF-8 coating significantly enhanced the photocatalytic performance of TiO2-ZnO. Pt/TiO2-ZnO@ZIF-8 decomposed 99.7 % of phenol after the corresponding solution was exposed to UV light for 24 min. This performance was significantly better than the phenol decomposition ability of TiO2-ZnO, Pt/TiO2-ZnO and TiO2, which degraded 76.1 %, 95.2 % and 86.9 % of phenol, respectively. Pt/TiO2-ZnO@ZIF-8 also demonstrated excellent recycling stability. All these properties, including photostability, made our novel Pt/TiO2-ZnO@ZIF-8 catalyst a promising material for practical applications in environmental remediation.

5.
Bioact Mater ; 7: 217-226, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34466728

RESUMO

With an upsurge of biodegradable metal implants, the research and application of Mg alloys in the gastrointestinal environment of the digestive tract have been of great interest. Digestive enzymes, mainly pepsin in the stomach and pancreatin in the small intestine, are widespread in the gastrointestinal tract, but their effect on the degradation of Mg alloys has not been well understood. In this study, we investigated the impacts of pepsin and pancreatin on the degradation of Mg-2Zn alloy wires. The results showed that the pepsin and pancreatin had completely different even the opposite effects on the degradation of Mg, although they both affected the degradation product layer. The degradation rate of Mg wire declined with the addition of pepsin in simulated gastric fluid (SGF) but rose with the addition of pancreatin in simulated intestinal fluid (SIF). The opposite trends in degradation rate also resulted in completely different degradation morphologies in wires surface, where the pitting corrosion in SGF was inhibited because of the physical barrier effect of pepsin adsorption. In contrast, the adsorption of pancreatin affected the integrity of magnesium hydrogen phosphate film, causing a relatively uneven degraded surface. These results may help us to understand the role of different digestive enzymes in the degradation of magnesium and facilitate the development and clinical application of magnesium alloy implanted devices for the digestive tract.

6.
Bioact Mater ; 8: 381-395, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34541408

RESUMO

This work was focused on study of anti-infection ability and its underlying mechanism of a novel dental implant made of titanium-copper (TiCu) alloy. In general, most studies on antibacterial implants have used a single pathogen to test their anti-infection ability using infectious animal models. However, dental implant-associated infections are polymicrobial diseases. We innovatively combine the classic ligature model in dogs with sucrose-rich diets to induce oral infections via the canine native oral bacteria. The anti-infection ability, biocompatibility and underlying mechanism of TiCu implant were systematically investigated in comparison with pure Ti implant via general inspection, hematology, imageology (micro-CT), microbiology (16S rDNA and metagenome), histology, and Cu ion detections. Compared with Ti implant, TiCu implant demonstrated remarkable anti-infection potentials with excellent biocompatibility. Additionally, the underlying anti-infection mechanism of TiCu implant was considered to involve maintaining the oral microbiota homeostasis. It was found that the carbohydrates in the plaques formed on the surface of TiCu implant were metabolized through the tricarboxylic acid cycle (TCA) cycles, which prevented the formation of an acidic microenvironment and inhibited the accumulation of acidogens and pathogens, thereby maintaining the microflora balance between aerobic and anaerobic bacteria.

7.
Biosens Bioelectron ; 195: 113586, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34455144

RESUMO

Enzyme-based biosensors are sensitive to temperature due to their strong temperature dependency of catalytic activity. Aiming at enhancing biosensing detection for glucose assay over a wide range of applicable temperatures, we designed a thermal self-regulatory intelligent biosensor through an innovative integration of phase change material (PCM) and bioelectrocatalytic substances. An electroactive phase-change microcapsule system was firstly fabricated by microencapsulating n-docosane as a PCM core in the SiO2 shell, followed by depositing polydopamine along with carbon nanotubes as an electroactive layer on the surface of SiO2 shell. The resultant microcapsules showed a regularly spherical morphology and well-defined core-shell microstructure. They also exhibited a satisfactory latent heat capacity of around 137 J/g for implementing temperature regulation with a good working stability. An electrochemical biosensing system was constructed with the resultant electroactive microcapsules together with glucose oxidase as a redox enzyme, achieving a thermal self-regulation capability to enhance the biosensing detection of glucose under in-situ thermal management at higher temperatures. With a high sensitivity of 5.95 µA⋅mM-1⋅cm-2 and a lower detection limit of 13.11 µM at 60 °C, the intelligent biosensor developed by this study demonstrated a superior determination capability and better detection performance toward glucose than conventional biosensors in a high temperature region thanks to effective regulation of microenvironment temperature in the electrode system. This study provides a promising strategy for the development of thermal self-regulatory smart biosensors with an enhanced identification ability to detect various chemical substances over a wide range of applicable temperatures.


Assuntos
Técnicas Biossensoriais , Nanotubos de Carbono , Cápsulas , Eletrodos , Enzimas Imobilizadas , Glucose , Glucose Oxidase , Dióxido de Silício
8.
Bioresour Technol ; 343: 126083, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34610429

RESUMO

The "Thermal-dissolution based carbon enrichment" was proven as an efficient and homogenizing treatment method in converting biomass wastes into similar high-quality carbon materials. However, their yields varied significantly with respect to the different experimental parameters employed. It is therefore imperative to establish the correlation between product yield and experimental parameters for material selection and condition optimization. In this study, Adaboost was coupled with an artificial neural network algorithm to precisely describe the abovementioned correlation. The results demonstrated the effectiveness of this model through its outstanding predicting performance for all the products, especially, the coefficient of determination in predicting the yield of Residue was as high as 0.97. Additionally, the coupling effect of temperature and time was observed. This study not only validates a close correlation between selected experimental parameters and product yields, but also provides a quick and reliable way for material selection and condition optimization.

9.
Spectrochim Acta A Mol Biomol Spectrosc ; 265: 120346, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34508928

RESUMO

Hg(II) is one of the most toxic heavy metal ions. The bioconcentration and degradation-resistant of Hg(II) bring about serious harm to the ecosystem and humans. Therefore, the establishment of an accurate and effective method for detecting mercury ions is of great significance to environmental protection, food safety and human health. In this work, a new fluorescent nanoprobe was presented using nitrogen-doped carbon quantum dots (N-CQDs) for Hg(II) sensing with high stability and selectivity. On this basis, a paper-based chip was innovatively developed for visualization detection of Hg(II). The N-CQDs were prepared through a one-step hydrothermal reaction using catechol and ethylenediamine as carbon and nitrogen sources, respectively. As-prepared N-CQDs exhibit the strong green fluorescence at the excitation/emission wavelength of 370/511 nm. In aqueous solution, a rapid and highly sensitive detection method of Hg(II) was established by the joint of dynamic and static quenching effect of Hg(II) on N-CQDs fluorescence. Under the optimized conditions, there was a stable correlation between the fluorescence intensity change of N-CQDs and the concentrations of Hg(II) in the range of 15 âˆ¼ 104 nM, and the detection limit was down to 8 nM (S/N = 3). The recoveries of water, sorghum and rice were 91.60 to 102.46%, which was consistent with ICP-MS. More importantly, the N-CQDs nanoprobe was further integrated in nitrocellulose membrane to develop paper-based chip for Hg(II) visualization detection, and the detection performance was also excellent. This strategy had significant implications for achieving low-cost, on-site real-time monitoring of mercury (II) in the environment and food.


Assuntos
Mercúrio , Pontos Quânticos , Carbono , Ecossistema , Humanos , Nitrogênio
10.
J Colloid Interface Sci ; 608(Pt 2): 1497-1513, 2021 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-34742069

RESUMO

Contamination and waste heat are major issues in water pollution. Aiming at efficient synchronous recovery wastewater and waste heat, we designed a novel CaCO3-based phase-change microcapsule system with an n-docosane core and a CaCO3/Fe3O4 composite shell. The system was fabricated through an emulsion-templated in situ precipitation approach in a structure-directing mode, resulting in a controllable morphology for the resultant microcapsules, varying from a peanut hull through ellipsoid to dumbbell shapes. The system has a significantly enlarged specific surface area of approximately 55 m2·g-1 with the CaCO3 phase transition from vaterite to calcite. As a result, the microcapsule system exhibits improved adsorption capacities of 497.6 and 79.1 mg/g for Pb2+ and Rhodamine B removal, respectively, from wastewater. Moreover, increase in the specific surface area of the microcapsule system with a sufficient latent heat capacity of approximately 130 J·g-1 also resulted in an enhanced heat energy-storage capability and thermal conductance for waste-heat recovery. The microcapsule system also exhibits a good leakage-prevention capability and good multicycle reusability owing to the tight magnetic CaCO3/Fe3O4 composite shell. This study provides a promising approach for developing CaCO3-based phase-change microcapsules with enhanced thermal energy storage and adsorption capabilities for efficient synchronous recovery of wastewater and waste heat.

11.
Rev Assoc Med Bras (1992) ; 67(9): 1317-1321, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34816927

RESUMO

OBJECTIVE: This study aimed to assess the attitudes of Chinese residents toward COVID-19 vaccines and explore the potential drivers for Chinese residents' vaccine hesitancy. METHODS: A cross-sectional survey was conducted from February 16 to March 16, 2021, by administering an online questionnaire to the Chinese residents. RESULTS: Of 5240 residents who completed the survey, 464 (8.9%) participants reported to have had one shot, and 348 (6.6%) reported to have had 2 shots. At the time the questionnaire was administered, 2298 (43.9%) participants reported they wanted to get vaccinated, while 2255 (43.0%) declared that they still did not know, and 687 (13.1%) respondents declared vaccine refusal. Overall, 2255 (43%) participants were categorized as vaccine hesitancy. Female participants (p=0.000), <20 years old (p=0.000), have low risk of COVID-19 (p=0.000) infection and strong associations of vaccine hesitancy. eHealth literacy was a protective factor. CONCLUSIONS: The results of this study show high rates of vaccine hesitancy in China. This could pose a serious threat to the preventive measures that aimed at controlling COVID-19 spread in the country. The government and different media platforms should encourage the dissemination of correct information about vaccines, the communities and medical staff to improve residents' knowledge about vaccines, and strive to improve residents' electronic health literacy.

12.
Rev Assoc Med Bras (1992) ; 67(7): 1010-1014, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34817515

RESUMO

OBJECTIVE: This study analyzes the suboptimal health status (SHS) and influencing factors of nurses in Wuhan Hospital, China during the coronavirus disease 2019 (COVID-19) outbreak. METHODS: This study was conducted through an online survey, from March 1-7, 2020, in Wuhan, China. The data collection tools, such as Suboptimal Health Status Questionnaires, Generalized Anxiety Disorder, and Chinese version of the Perceived Stress Scale, were used. RESULTS: The average value of suboptimal health status was 28.44 (standard deviation=15.15). The overall prevalence of SHS was 35.1%. Suboptimal health status of the nurses was significantly different based on their gender, age, whether they directly care for COVID-19 patients, anxiety level, and stress perception expect education. Multivariate analysis found that average sleep times per day, female, age, directly participate in the rescue of COVID-19, self-infection, and anxiety were the influencing factors of suboptimal health status. CONCLUSIONS: First-line nurses have poor suboptimal health status in Wuhan.

13.
Ann Transl Med ; 9(19): 1502, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34805364

RESUMO

Objective: This article focused on the application scenarios of three-dimensional (3D) bioprinting and gene-editing technology in various medical fields, including gene therapy, tissue engineering, tumor microenvironment simulation, tumor model construction, cancer regulation and expression, osteogenesis, and skin and vascular regeneration, and summarizing its development prospects and shortcomings. Background: 3D bioprinting is a process based on additive manufacturing that uses biological materials as the microenvironment living cells. The scaffolds and carriers manufactured by 3D bioprinting technology provide a safe, efficient, and economical platform for genes, cells, and biomolecules. Gene modification refers to replacing, splicing, silencing, editing, controlling or inactivating genes and delivering new genes. The combination of this technology that changes cell function or cell fate or corrects endogenous mutations and 3D bioprinting technology has been widely used in various medical field. Methods: We conducted a literature search for papers published up to March 2021 on the gene modification combined with 3D bioprinting in various medical fields via PubMed, Web of Science, China National Knowledge Infrastructure (CNKI). The following medical subject heading terms were included for a MEDLINE search: "3D printing/gene editing", "3D printing/genetic modification", "3D printing/seed cell", "bioprinting/gene editing", "bioprinting/genetic modification", "bioprinting/seed cell", "scaffold/gene editing", "scaffold/genetic modification", "scaffold/seed cell", "gene/scaffold", "gene/bioprinting", "gene/3D printing". Quantitative and qualitative data was extracted through interpretation of each article. Conclusions: We have reviewed the application scenarios of 3D bioprinting and gene-editing technology in various medical fields, it provides an efficient and accurate delivery system for personalized tumor therapy, enhancing the targeting effect while maintaining the integrity of the fabricated structure. It exhibits significant application potential in developing tumor drugs. In addition, scaffolds obtained via 3D bioprinting provide gene therapy applications for skin and bone healing and repair and inducing stem cell differentiation. It also considers the future development direction in this field, such as the emergence and development of gene printing, 4D printing. The combination of nanotechnology and gene printing may provide a new way for future disease research and treatment.

14.
Immun Ageing ; 18(1): 42, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34740366

RESUMO

BACKGROUND: Toll-like receptor 4 (TLR4) is a pattern recognition receptor of the innate immune system. TLR4 contributes to many aging-related chronic diseases. However, whether TLR4 is involved in cardiovascular injury during the aging process has not been investigated. METHODS: The effects of TLR4 on the cardiovascular system of aged mice were investigated in TLR4-/- mice. An intraperitoneal glucose tolerance test (IPGTT) and insulin sensitivity test (IST) were conducted to evaluate global insulin sensitivity. Echocardiography was used to measure cardiac structure and performance. An isolated artery ring assay was used to measure the vasodilator function of the thoracic aorta. The inflammatory response was reflected by the serum concentration of cytokines. RESULTS: TLR4 expression increased in the hearts and aortas of mice in an age-dependent manner. Loss of TLR4 increased insulin sensitivity in aged mice. Moreover, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. Importantly, the increases in serum inflammatory cytokines and oxidative stress in the heart and aorta were also inhibited by TLR4 deficiency. CONCLUSION: In summary, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. The reduced inflammatory responses and oxidative stress may be the reason for the protective effects of TLR4 deficiency during aging. Our study indicates that targeting TLR4 is a potential therapeutic strategy for preventing aging-related cardiovascular disease.

15.
Chemosphere ; : 132909, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34785180

RESUMO

Although previous meta-analyses have shown that prenatal PFASs exposure is associated with reduction in birth weight, effects of prenatal PFASs exposure on birth outcomes have not been fully explored. We conducted a meta-analysis of 23 eligible studies searched from Embase, PubMed, and Web of Science before March 21, 2021 to analyze the association between prenatal PFASs exposure and birth outcomes, including premature birth (PTB), low birth weight (LBW), small for gestational age (SGA) and miscarriage. Odds ratio (OR) and corresponding confidence intervals were extracted for analysis. According to the heterogeneity of the included studies, fixed-effects (I2 ≤ 50%) and random-effects (I2 > 50%) models were applied respectively. The significant associations between PFOS and PTB (pooled OR = 1.54, 95% CI: 1.20-1.98), PFOA and miscarriage (pooled OR = 1.40, 95% CI: 1.15-1.70), and PFOS and LBW (pooled OR = 1.52, 95% CI: 1.19-1.94) were obtained. There were differences between included studies with different study regions, sampling time, and samples type used for PFASs assessment. These findings may provide insight in risk assessment and decision-making in producing products that contain PFASs.

16.
Pest Manag Sci ; 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34773351

RESUMO

BACKGROUND: Bactrocera dorsalis is a devastating pest on fruits and vegetables because the adult female is the key factor that determines the population density of offspring and the degree of host damage. Unfortunately, there is still a lack of effective female attractants for behavioral control. Males of B. dorsalis fed on methyl eugenol (ME) were shown to be more sexually attracted to females and, therefore, were more successful in mating over ME-deprived males. RESULTS: In the current study, we demonstrated that (E)-coniferyl alcohol (E-CF), one of the ME metabolites in males, was highly attractive to sexually-mature females in laboratory bioassays. During the dusk courtship period, mature females showed the highest response to E-CF. However, there were no significant differences in olfactory responses to E-CF between virgin and mated mature females. Moreover, no obvious signs and symptoms of toxicity or death were observed in mice during a 14-day acute oral toxicity test. Toxicologically, no significant changes were observed in body weight, water intake, food consumption and absolute and relative organ weights between control and treated groups of healthy-looking mice, implying that E-CF could be regarded as non-toxic. Furthermore, cytotoxicity assessment revealed that E-CF was non-toxic against human fetal lung fibroblast 1 (HFL1), human breast cancer (MDA-MB-231), mouse embryonic hepatocytes (BNL-CL.2) and Spodoptera frugiperda ovary (SF-9) cell lines. CONCLUSIONS: E-CF proved to be an effective, promising and eco-friendly lure to B. dorsalis females. Therefore, this study may facilitate the development of novel control strategies against B. dorsalis in the field.

17.
Artigo em Inglês | MEDLINE | ID: mdl-34807500

RESUMO

Nanoparticle aggregation for constructing functional materials has shown enormous advantages in various applications. Most efforts focused on ordered nanoparticle aggregation for specific functions but often limited in irreversible aggregation processes due to the thermodynamic equilibrium. Herein, we report a reversible disordered aggregation of SiO 2 -PNIPAAm nanoparticles (SPNPs) through thermo-responsive jamming, obtaining smart liquid-solid switchable materials. The smart materials can display a switch between liquid-like state and solid-like state responding to temperature change. This unique macroscopic behavior originates from the reversible disordered aggregation modulated by temperature-dependent hydrophobic interactions among the SPNPs. Notably, the materials at solid-like state show anti-impact property and can withstand the impact of a steel sphere with a speed of 328 cm/s. We envision that this finding offers inspirations to design smart liquid-solid switchable materials for impact protection.

18.
BMC Psychiatry ; 21(1): 572, 2021 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-34781901

RESUMO

BACKGROUND: The COVID-19 pandemic has posed significant threats to both the physical and psychological health of healthcare workers working in the front-line combating COVID-19. However, studies regarding the medium to long term impact of COVID-19 on mental health among healthcare workers are limited. Therefore, we conducted this cross-sectional survey to investigate the prevalence, factors and impact of post-traumatic stress disorder (PTSD) in healthcare workers exposed to COVID-19 8 months after the end of the outbreak in Wuhan, China. METHODS: A web-based questionnaire was delivered as a link via the communication application WeChat to those healthcare workers who worked at several COVID-19 units during the outbreak (from December 2019 to April 2020) in Wuhan, China. The questionnaire included questions on social-demographic data, the post-traumatic stress disorder checklist-5 (PCL-5), the family care index questionnaire (Adaptation, Partnership, Growth, Affection and Resolve, APGAR), and the quality-of-life scale (QOL). The prevalence, risk and protective factors, and impact of PTSD on healthcare workers were subsequently analyzed. RESULTS: Among the 659 participants, 90 healthcare workers were still suffering from PTSD 8 months after the end of the outbreak of COVID-19 in Wuhan, in which avoidance and negative impact were the most affected dimensions. Suffering from chronic disease, experiencing social isolation, and job dissatisfaction came up as independent risk factors for PTSD, while obtaining COVID-19 related information at an appropriate frequency, good family function, and working in well-prepared mobile cabin hospitals served as protective factors. The impact of PTSD on COVID-19 exposed healthcare workers was apparent by shortened sleeping time, feeling of loneliness, poorer quality of life and intention to resign. CONCLUSIONS: Eight months after the end of the COVID-19 outbreak in Wuhan, the level of PTSD in healthcare workers exposed to COVID-19 was still high. Apart from the commonly recognized risk factors, comorbid chronic disease was identified as a new independent risk factor for developing PTSD. For countries where the pandemic is still ongoing or in case of future outbreaks of new communicable diseases, this study may contribute to preventing cases of PTSD in healthcare workers exposed to infectious diseases under such circumstances.


Assuntos
COVID-19 , Transtornos de Estresse Pós-Traumáticos , China/epidemiologia , Estudos Transversais , Pessoal de Saúde , Humanos , Pandemias , Prevalência , Qualidade de Vida , SARS-CoV-2 , Transtornos de Estresse Pós-Traumáticos/epidemiologia
19.
Am J Transl Res ; 13(10): 11107-11125, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34786046

RESUMO

The biomarker p16 plays a role in aging and is upregulated in aged organs and cells, including bone marrow mesenchymal stem cells (BM-MSCs), which play a leading role in fracture healing. Several studies have reported delayed fracture healing in geriatric mice. However, the relationship between p16 expression and fracture healing in geriatric mice remains poorly understood. In this study, we found that fracture healing was accelerated in p16 deletion (p16-/-) mice, and the number of migrated BM-MSCs from p16-/- mice increased. The expressions of SDF-1 and CXCR4 were also upregulated in p16-/- mice. Increased cell percentage at S phase in cell cycle, enhanced expressions of CDK4/6, pRB, and E2F1, decreased expression of RB, and elevated expressions of SOX9, PCNA, and COL2A1 were detected in p16-/- mice. The expressions of COL10A1, MMP13, OSTERIX, and COL1A1 were also high in p16-/- mice. Moreover, the expressions of p-AKT, p-mTOR, HIF-1α, and VEGF-A in BM-MSCs and expression of VEGF-A in callus were upregulated in p16-/- mice. The expression of VEGF in the serum of p16-/- mice was also higher than that of wild type mice. Thus, deletion of p16 enhances migration, division, and differentiation of BM-MSCs, promotes proliferation and maturation of chondrocytes, activates osteoblastogenesis, and facilitates vascularization to accelerate fracture healing, providing a novel strategy to treat fracture in the elderly.

20.
Health Qual Life Outcomes ; 19(1): 254, 2021 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-34772424

RESUMO

OBJECTIVE: Elderly patients with type 2 diabetes mellitus are highly vulnerable due to severe complications. However, there is a contradiction in the relationship between social support and quality of life, which warrants further exploration of the internal mechanism. This study assessed the quality of life and its interfering factors in this patient population. METHODS: In total, 571 patients with type 2 diabetes mellitus over 60 years old were recruited from two community clinics in Heilongjiang Province, China. We collected data on health status, quality of life, self-management behavior, fasting plasma glucose (FPG) level, and social support. Structural equation modeling and the bootstrap method were used to analyze the data. RESULTS: The average quality of life score was - 29.25 ± 24.41. Poorly scored domains of quality of life were "Psychological feeling" (- 8.67), "Activity" (- 6.36), and "Emotion" (- 6.12). Of the 571 patients, 65.32% had normal FPG, 9.8% had high-risk FPG, 15.94% had good self-management behavior, and 22.07% had poor social support. Significant correlations among social support, self-management behavior, FPG level, and quality of life were noted. A multiple mediator model revealed that social support influenced quality of life in three ways: (1) directly (c' = 0.6831); (2) indirectly through self-management behavior (a1*b1 = 0.1773); and (3) indirectly through FPG control (a2*b2 = 0.1929). Self-management behavior influenced the quality of life directly and indirectly through FPG control. CONCLUSION: Improving self-management behavior and monitoring hypoglycemia should become priority targets for future intervention. Scheduled social support to self-management projects should be put into the standardized management procedure. Physicians should provide substantial and individualized support to the elderly patients with type 2 diabetes mellitus regarding medication, blood glucose monitoring, and physical exercise.


Assuntos
Diabetes Mellitus Tipo 2 , Autogestão , Idoso , Glicemia , Automonitorização da Glicemia , Diabetes Mellitus Tipo 2/terapia , Jejum , Humanos , Pessoa de Meia-Idade , Qualidade de Vida , Apoio Social
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...